CN109402003B - Microbial agent for sludge reduction, carrier thereof and preparation method of carrier - Google Patents
Microbial agent for sludge reduction, carrier thereof and preparation method of carrier Download PDFInfo
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- CN109402003B CN109402003B CN201811293620.3A CN201811293620A CN109402003B CN 109402003 B CN109402003 B CN 109402003B CN 201811293620 A CN201811293620 A CN 201811293620A CN 109402003 B CN109402003 B CN 109402003B
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- 230000000813 microbial effect Effects 0.000 title claims abstract description 33
- 239000003795 chemical substances by application Substances 0.000 title claims abstract description 25
- 239000010802 sludge Substances 0.000 title claims abstract description 21
- 238000002360 preparation method Methods 0.000 title claims abstract description 16
- 230000009467 reduction Effects 0.000 title claims abstract description 12
- VNWKTOKETHGBQD-UHFFFAOYSA-N methane Chemical compound C VNWKTOKETHGBQD-UHFFFAOYSA-N 0.000 claims abstract description 20
- 241000588813 Alcaligenes faecalis Species 0.000 claims abstract description 7
- 241000193744 Bacillus amyloliquefaciens Species 0.000 claims abstract description 7
- 241000194108 Bacillus licheniformis Species 0.000 claims abstract description 7
- 244000063299 Bacillus subtilis Species 0.000 claims abstract description 7
- 235000014469 Bacillus subtilis Nutrition 0.000 claims abstract description 7
- 241000186220 Cellulomonas flavigena Species 0.000 claims abstract description 7
- 241000193401 Clostridium acetobutylicum Species 0.000 claims abstract description 7
- 240000001046 Lactobacillus acidophilus Species 0.000 claims abstract description 7
- 235000013956 Lactobacillus acidophilus Nutrition 0.000 claims abstract description 7
- 241000605122 Nitrosomonas Species 0.000 claims abstract description 7
- 241000235645 Pichia kudriavzevii Species 0.000 claims abstract description 7
- 241000190950 Rhodopseudomonas palustris Species 0.000 claims abstract description 7
- 241000192023 Sarcina Species 0.000 claims abstract description 7
- 241000194020 Streptococcus thermophilus Species 0.000 claims abstract description 7
- 241000187747 Streptomyces Species 0.000 claims abstract description 7
- 229940005347 alcaligenes faecalis Drugs 0.000 claims abstract description 7
- 229940039695 lactobacillus acidophilus Drugs 0.000 claims abstract description 7
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 46
- 239000003999 initiator Substances 0.000 claims description 13
- SMZOUWXMTYCWNB-UHFFFAOYSA-N 2-(2-methoxy-5-methylphenyl)ethanamine Chemical compound COC1=CC=C(C)C=C1CCN SMZOUWXMTYCWNB-UHFFFAOYSA-N 0.000 claims description 11
- NIXOWILDQLNWCW-UHFFFAOYSA-N 2-Propenoic acid Natural products OC(=O)C=C NIXOWILDQLNWCW-UHFFFAOYSA-N 0.000 claims description 11
- 241000894006 Bacteria Species 0.000 claims description 10
- 238000010438 heat treatment Methods 0.000 claims description 10
- 229910017059 organic montmorillonite Inorganic materials 0.000 claims description 10
- 238000005406 washing Methods 0.000 claims description 10
- LZZYPRNAOMGNLH-UHFFFAOYSA-M Cetrimonium bromide Chemical compound [Br-].CCCCCCCCCCCCCCCC[N+](C)(C)C LZZYPRNAOMGNLH-UHFFFAOYSA-M 0.000 claims description 9
- 229920002558 Curdlan Polymers 0.000 claims description 9
- 239000001879 Curdlan Substances 0.000 claims description 9
- OKKJLVBELUTLKV-UHFFFAOYSA-N Methanol Chemical compound OC OKKJLVBELUTLKV-UHFFFAOYSA-N 0.000 claims description 9
- HEMHJVSKTPXQMS-UHFFFAOYSA-M Sodium hydroxide Chemical compound [OH-].[Na+] HEMHJVSKTPXQMS-UHFFFAOYSA-M 0.000 claims description 9
- WQZGKKKJIJFFOK-VFUOTHLCSA-N beta-D-glucose Chemical compound OC[C@H]1O[C@@H](O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-VFUOTHLCSA-N 0.000 claims description 9
- 229940078035 curdlan Drugs 0.000 claims description 9
- 235000019316 curdlan Nutrition 0.000 claims description 9
- 238000001291 vacuum drying Methods 0.000 claims description 9
- 238000005303 weighing Methods 0.000 claims description 9
- 239000003431 cross linking reagent Substances 0.000 claims description 8
- 239000000126 substance Substances 0.000 claims description 8
- CSCPPACGZOOCGX-UHFFFAOYSA-N Acetone Chemical compound CC(C)=O CSCPPACGZOOCGX-UHFFFAOYSA-N 0.000 claims description 6
- VEXZGXHMUGYJMC-UHFFFAOYSA-N Hydrochloric acid Chemical compound Cl VEXZGXHMUGYJMC-UHFFFAOYSA-N 0.000 claims description 6
- 238000006243 chemical reaction Methods 0.000 claims description 6
- 239000012153 distilled water Substances 0.000 claims description 6
- 239000002904 solvent Substances 0.000 claims description 6
- 239000000725 suspension Substances 0.000 claims description 6
- 238000003756 stirring Methods 0.000 claims description 5
- 238000006386 neutralization reaction Methods 0.000 claims description 4
- 238000001816 cooling Methods 0.000 claims description 3
- 239000008367 deionised water Substances 0.000 claims description 3
- 229910021641 deionized water Inorganic materials 0.000 claims description 3
- GUJOJGAPFQRJSV-UHFFFAOYSA-N dialuminum;dioxosilane;oxygen(2-);hydrate Chemical compound O.[O-2].[O-2].[O-2].[Al+3].[Al+3].O=[Si]=O.O=[Si]=O.O=[Si]=O.O=[Si]=O GUJOJGAPFQRJSV-UHFFFAOYSA-N 0.000 claims description 3
- IDGUHHHQCWSQLU-UHFFFAOYSA-N ethanol;hydrate Chemical compound O.CCO IDGUHHHQCWSQLU-UHFFFAOYSA-N 0.000 claims description 3
- 229920001519 homopolymer Polymers 0.000 claims description 3
- GBMDVOWEEQVZKZ-UHFFFAOYSA-N methanol;hydrate Chemical compound O.OC GBMDVOWEEQVZKZ-UHFFFAOYSA-N 0.000 claims description 3
- 239000000178 monomer Substances 0.000 claims description 3
- 229910052901 montmorillonite Inorganic materials 0.000 claims description 3
- ZIUHHBKFKCYYJD-UHFFFAOYSA-N n,n'-methylenebisacrylamide Chemical compound C=CC(=O)NCNC(=O)C=C ZIUHHBKFKCYYJD-UHFFFAOYSA-N 0.000 claims description 3
- 239000002245 particle Substances 0.000 claims description 3
- 238000000926 separation method Methods 0.000 claims description 3
- 238000007873 sieving Methods 0.000 claims description 3
- 238000000967 suction filtration Methods 0.000 claims description 3
- 238000001514 detection method Methods 0.000 claims description 2
- 239000011837 N,N-methylenebisacrylamide Substances 0.000 claims 1
- 239000010865 sewage Substances 0.000 abstract description 31
- 230000000694 effects Effects 0.000 abstract description 9
- 241001074903 Methanobacteria Species 0.000 abstract description 5
- 230000008021 deposition Effects 0.000 abstract description 3
- 244000005700 microbiome Species 0.000 description 12
- 238000000034 method Methods 0.000 description 8
- 239000002068 microbial inoculum Substances 0.000 description 8
- 238000011144 upstream manufacturing Methods 0.000 description 6
- 238000011049 filling Methods 0.000 description 5
- 239000007789 gas Substances 0.000 description 4
- 238000004065 wastewater treatment Methods 0.000 description 4
- 241001052560 Thallis Species 0.000 description 3
- 239000000969 carrier Substances 0.000 description 3
- 230000003247 decreasing effect Effects 0.000 description 3
- 238000002474 experimental method Methods 0.000 description 3
- 238000002156 mixing Methods 0.000 description 3
- 239000000843 powder Substances 0.000 description 3
- 239000013049 sediment Substances 0.000 description 3
- QGZKDVFQNNGYKY-UHFFFAOYSA-N Ammonia Chemical compound N QGZKDVFQNNGYKY-UHFFFAOYSA-N 0.000 description 2
- RWSOTUBLDIXVET-UHFFFAOYSA-N Dihydrogen sulfide Chemical compound S RWSOTUBLDIXVET-UHFFFAOYSA-N 0.000 description 2
- XEEYBQQBJWHFJM-UHFFFAOYSA-N Iron Chemical compound [Fe] XEEYBQQBJWHFJM-UHFFFAOYSA-N 0.000 description 2
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 description 2
- 230000009286 beneficial effect Effects 0.000 description 2
- 230000007613 environmental effect Effects 0.000 description 2
- 229910000037 hydrogen sulfide Inorganic materials 0.000 description 2
- 238000013048 microbiological method Methods 0.000 description 2
- 230000003472 neutralizing effect Effects 0.000 description 2
- 229910052760 oxygen Inorganic materials 0.000 description 2
- 239000001301 oxygen Substances 0.000 description 2
- 238000012856 packing Methods 0.000 description 2
- 238000000053 physical method Methods 0.000 description 2
- 230000000087 stabilizing effect Effects 0.000 description 2
- 230000004083 survival effect Effects 0.000 description 2
- 241000186000 Bifidobacterium Species 0.000 description 1
- MBMLMWLHJBBADN-UHFFFAOYSA-N Ferrous sulfide Chemical compound [Fe]=S MBMLMWLHJBBADN-UHFFFAOYSA-N 0.000 description 1
- 206010021143 Hypoxia Diseases 0.000 description 1
- PWHULOQIROXLJO-UHFFFAOYSA-N Manganese Chemical compound [Mn] PWHULOQIROXLJO-UHFFFAOYSA-N 0.000 description 1
- 229920002125 Sokalan® Polymers 0.000 description 1
- NINIDFKCEFEMDL-UHFFFAOYSA-N Sulfur Chemical compound [S] NINIDFKCEFEMDL-UHFFFAOYSA-N 0.000 description 1
- 238000010521 absorption reaction Methods 0.000 description 1
- 150000001412 amines Chemical class 0.000 description 1
- 229910021529 ammonia Inorganic materials 0.000 description 1
- 239000012298 atmosphere Substances 0.000 description 1
- 238000010170 biological method Methods 0.000 description 1
- 238000004140 cleaning Methods 0.000 description 1
- 239000002131 composite material Substances 0.000 description 1
- 150000001875 compounds Chemical class 0.000 description 1
- 238000000354 decomposition reaction Methods 0.000 description 1
- 230000007547 defect Effects 0.000 description 1
- 238000011161 development Methods 0.000 description 1
- 230000029087 digestion Effects 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 229910001385 heavy metal Inorganic materials 0.000 description 1
- 238000011065 in-situ storage Methods 0.000 description 1
- 239000010842 industrial wastewater Substances 0.000 description 1
- 229910052742 iron Inorganic materials 0.000 description 1
- 229910052748 manganese Inorganic materials 0.000 description 1
- 239000011572 manganese Substances 0.000 description 1
- 230000004060 metabolic process Effects 0.000 description 1
- 239000002207 metabolite Substances 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 239000008239 natural water Substances 0.000 description 1
- 230000035699 permeability Effects 0.000 description 1
- 239000004584 polyacrylic acid Substances 0.000 description 1
- 239000011148 porous material Substances 0.000 description 1
- 230000008569 process Effects 0.000 description 1
- 230000035755 proliferation Effects 0.000 description 1
- 238000000746 purification Methods 0.000 description 1
- 239000008213 purified water Substances 0.000 description 1
- 230000000630 rising effect Effects 0.000 description 1
- 238000001179 sorption measurement Methods 0.000 description 1
- 229910052717 sulfur Inorganic materials 0.000 description 1
- 239000011593 sulfur Substances 0.000 description 1
- 230000002195 synergetic effect Effects 0.000 description 1
Classifications
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/20—Bacteria; Culture media therefor
-
- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F11/00—Treatment of sludge; Devices therefor
- C02F11/02—Biological treatment
-
- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F3/00—Biological treatment of water, waste water, or sewage
- C02F3/34—Biological treatment of water, waste water, or sewage characterised by the microorganisms used
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/14—Fungi; Culture media therefor
- C12N1/16—Yeasts; Culture media therefor
-
- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F2303/00—Specific treatment goals
- C02F2303/06—Sludge reduction, e.g. by lysis
-
- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F2305/00—Use of specific compounds during water treatment
- C02F2305/06—Nutrients for stimulating the growth of microorganisms
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- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Health & Medical Sciences (AREA)
- Organic Chemistry (AREA)
- Microbiology (AREA)
- Biotechnology (AREA)
- Zoology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Wood Science & Technology (AREA)
- Genetics & Genomics (AREA)
- Tropical Medicine & Parasitology (AREA)
- Water Supply & Treatment (AREA)
- Virology (AREA)
- Biochemistry (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Medicinal Chemistry (AREA)
- Mycology (AREA)
- Hydrology & Water Resources (AREA)
- Environmental & Geological Engineering (AREA)
- Biomedical Technology (AREA)
- Molecular Biology (AREA)
- Biodiversity & Conservation Biology (AREA)
- Botany (AREA)
- Biological Treatment Of Waste Water (AREA)
Abstract
The invention discloses a microbial agent for sludge reduction, which comprises the following components in parts by weight: 8-10 parts of clostridium acetobutylicum, 12-16 parts of bacillus licheniformis, 16-20 parts of bacillus subtilis, 5-7 parts of lactobacillus acidophilus, 5-7 parts of methane sarcina, 3-5 parts of methanobacteria mane, 6-8 parts of rhodopseudomonas palustris, 4-6 parts of cellulomonas flavigena, 8-10 parts of streptococcus thermophilus, 8-10 parts of alcaligenes faecalis, 3-5 parts of bacillus amyloliquefaciens, 2-4 parts of nitrosomonas nitrosification, 1-3 parts of issatchenkia orientalis and 3-5 parts of streptomyces bovis; meanwhile, the carrier of the microbial agent for sludge reduction and the preparation method thereof are disclosed, and the carrier can effectively attach the microbial agent so that the microbial agent has better activity. The strain in the microbial agent is reasonably prepared, is attached to a carrier for sewage treatment, and is simple, good in treatment effect, less in sludge deposition and worthy of popularization.
Description
Technical Field
The invention belongs to the technical field of sewage treatment, and particularly relates to a microbial agent for sludge reduction, a carrier thereof and a preparation method of the carrier.
Background
Water is a source of human life and cannot be replaced, but the discharge of industrial wastewater and domestic sewage at present enters natural water bodies, so that various water bodies cause large-area and large-range pollution. The decomposition of organic matters in the polluted water consumes a large amount of dissolved oxygen, and the water is seriously anoxic or anaerobic, so that the river is blacked and smelly finally. River black odor is one of water body pollution, in an anoxic water body, anaerobic microorganisms decompose organic matters to generate a large amount of odorous gases such as methane, hydrogen sulfide, ammonia, amine and other volatile small molecular compounds with peculiar smell, and the odorous gases escape from the water surface and enter the atmosphere to cause the water body to smell; at the same time, CH is generated in the deposit4、N2、H2S and other gases which are difficult to dissolve in water carry sludge into the water phase in the rising process, so that the water body is blackened. The oxygen deficiency of the water body also causes the reduction of heavy metals such as iron, manganese and the like in the water, and forms ferrous sulfide with sulfur in the water, and the like, which has the dominant effect in the blackening effect of the water body. For the treatment of sewage, the current common methods include a physical method, a chemical method and a biological method, and the physical method and the chemical method can not fundamentally solve the problem of black and odorous river water. Therefore, with the increasing environmental requirements and the continuous development of environmental biotechnology, bioremediation is used as a low-investment, high-benefit, operational practiceAs a convenient and flexible water body pollution treatment technology, a microbiological method is usually adopted for bioremediation to treat polluted water bodies, and a plurality of microbiological methods all adopt a single biological bacterium to treat the polluted water bodies, so that the survival rate and the activity of the microbial bacterium are low, and the self-cleaning capacity of the water bodies is difficult to recover. Therefore, the composite microorganism is adopted to treat the sewage, and a better treatment effect can be achieved.
Disclosure of Invention
The first object of the present invention is: aiming at the defects in the prior art, the invention provides the microbial agent for sludge reduction, the adaptability of strains is strong, the survival rate is high, organic matters and inorganic matters in sewage are digested, absorbed or converted through the synergistic action of various microorganisms, the microbial agent can clean water bodies and river sediment without silt, the sludge can be reduced by about 70%, the color of the sludge is changed from black to grey, and the aim of sewage purification treatment is fulfilled.
The second purpose of the invention is to provide a carrier of the microbial agent for sewage treatment and a preparation method thereof, wherein the carrier is made of porous materials, and can effectively attach the microbial bacteria, so that the microbial bacteria have better activity and are relatively fixed in situ; the preparation method is simple, and the prepared carrier has uniform gaps and good adsorbability.
In order to solve the technical problems, the technical scheme of the invention is as follows:
a microbial agent for sludge reduction comprises the following components in parts by weight: 8-10 parts of clostridium acetobutylicum, 12-16 parts of bacillus licheniformis, 16-20 parts of bacillus subtilis, 5-7 parts of lactobacillus acidophilus, 5-7 parts of methane sarcina, 3-5 parts of methanobacteria mane, 6-8 parts of rhodopseudomonas palustris, 4-6 parts of cellulomonas flavigena, 8-10 parts of streptococcus thermophilus, 8-10 parts of alcaligenes faecalis, 3-5 parts of bacillus amyloliquefaciens, 2-4 parts of nitrosomonas nitrosification, 1-3 parts of issatchenkia orientalis and 3-5 parts of streptomyces bovis. Each gram of the microbial inoculum contains more than 5 million of active bacteria.
Preferably, the microbial agent comprises the following components in parts by weight: 9 parts of clostridium acetobutylicum, 14 parts of bacillus licheniformis, 18 parts of bacillus subtilis, 6 parts of lactobacillus acidophilus, 6 parts of methane sarcina, 4 parts of mane methane bacteria, 7 parts of rhodopseudomonas palustris, 5 parts of cellulomonas flavigena, 9 parts of streptococcus thermophilus, 9 parts of alcaligenes faecalis, 4 parts of bacillus amyloliquefaciens, 3 parts of nitrosomonas nitrosum, 2 parts of issatchenkia orientalis and 4 parts of streptomyces bullatae.
A preparation method of a carrier of a microbial agent for sludge reduction comprises the steps of grafting curdlan with polyacrylic acid and organic montmorillonite in a weight ratio of 10: 0.2, and further comprising a small amount of an initiator (1.5% of the weight of the curdlan) and a crosslinking agent (0.1% of the weight of the curdlan);
the preparation method of the carrier of the microbial agent for sludge reduction comprises the following steps:
a. preparing organic montmorillonite: weighing 20g of montmorillonite to prepare a suspension with the weight percentage of 10%, adding the suspension into a three-neck flask, adjusting the pH value to 6.0-6.5 by using 0.2mol/L hydrochloric acid, starting heating and stirring, weighing 10g of hexadecyl trimethyl ammonium bromide, dissolving the hexadecyl trimethyl ammonium bromide in water, slightly heating to completely dissolve the hexadecyl trimethyl ammonium bromide, pouring the solution into a dropping funnel, dropwise adding the solution into the three-neck flask, heating for a period of time, cooling to room temperature, performing centrifugal separation, performing suction filtration, and washing for 3 times; crushing the obtained product in a vacuum drying oven at 60 ℃ for 12h by using a crusher, and sieving the crushed product by using a 120-mesh sieve to obtain organic montmorillonite;
b. preparation of a crude carrier: weighing 2g of curdlan, adding the curdlan into a three-neck flask, adding 8g of acrylic acid into a 50ml small beaker by using a pipette, neutralizing the acrylic acid to a certain neutralization degree by using 30% NaOH solution, adding the acrylic acid into the three-neck flask, respectively dissolving 0.03g of initiator and 0.002g of cross-linking agent into 10ml of distilled water, adding the acrylic acid into three dropping funnels, firstly dropping 1/3 of initiator solution, stirring for 10min, then beginning dropping the initiator solution, controlling the dropping speed of the initiator solution (the dropping is finished after 2 h), beginning dropping the cross-linking agent solution after 1h (the dropping is finished), adding 0.2g of organic montmorillonite after the dropping is finished, continuing to react for 2h, finishing the reaction, washing the product by using 1:4 (volume ratio) water-methanol solution for 3 times, placing the product into a vacuum drying oven at 60 ℃ for 12h, and crushing the product by using a crusher for later use;
c. preparing a carrier finished product: packing the crushed product with filter paper, putting the packed product into a Soxhlet extractor, firstly extracting for 12h by using methanol as a solvent, then extracting for 12h by using acetone as a solvent to remove small molecular substances such as acrylic acid homopolymer, residual monomers and the like, repeatedly washing the extracted residue with distilled water to obtain a purified porous carrier finished product, and crushing the carrier finished product to a certain particle size for later use by a crusher after 12h in a vacuum drying oven at 60 ℃.
Preferably, the heating and stabilizing time in the step a is 80 ℃ for reaction for 20 h.
Preferably, the washing in the step a is 3 times of washing with deionized water and a 50% ethanol-water solution (volume fraction), respectively.
Preferably, the neutralization degree in the step b is 70%.
Preferably, the initiator in step b is (NH)4)2S2O8The cross-linking agent is N, N' -methylene bisacrylamide.
The working principle is as follows:
the prepared microbial inoculum is uniformly mixed into a microbial inoculum carrier, then a large amount of carriers are put into a corresponding reticular container, sewage slowly passes through the reticular container, organic matters and inorganic matters in the sewage are purified by digestion, absorption, conversion or adsorption of microorganisms on the carriers, the effluent water is the purified water after BOD and COD are reduced, and the odor, the clarity and the like of the effluent water are greatly improved. After a period of treatment, activated sludge or residues can be generated due to the growth of thalli and the like, the flow of the water body is influenced, and the microbial inoculum and the carrier can be replaced to carry out the next sewage treatment again.
Due to the adoption of the technical scheme, the invention has the beneficial effects that:
1. the porous carrier contains curdlan components, and the curdlan can promote the growth of microorganisms, is particularly beneficial to the proliferation of bifidobacteria, so that thalli can be promoted to be rapidly bred, and sewage treatment can be better carried out;
2. the gaps in the carrier can better attach various microorganisms, the air permeability is strong, oxygen and the like required by the growth of the microorganisms can be provided, and gas generated by the metabolism of the microorganisms can be effectively discharged, so that the influence of metabolites on the growth of the microorganisms is avoided;
3. the microbial agent contains aerobic microorganisms and anaerobic microorganisms, has various types of microorganisms, can degrade or convert various organic matters and inorganic matters, and can be suitable for the growth of thalli in an anaerobic environment with higher sewage degree and an aerobic environment with lower sewage degree, so that sewage with different degrees can be treated.
4. The invention not only purifies the water, but also greatly influences the sludge at the bottom of the river, greatly reduces the sludge deposition amount and ensures the smooth flow of the water body.
In a word, the strain in the microbial agent is reasonably prepared, can be well attached to a carrier for sewage treatment, and is simple in method, good in treatment effect and worthy of popularization.
Detailed Description
The invention is further illustrated by the following examples.
Example onePreparation of microbial agent
The method comprises the following steps of fully mixing the following component bacteria powder in parts by weight, 8 parts of clostridium acetobutylicum, 16 parts of bacillus licheniformis, 16 parts of bacillus subtilis, 7 parts of lactobacillus acidophilus, 5 parts of methane sarcina, 5 parts of methanobacteria mane, 6 parts of rhodopseudomonas palustris, 4 parts of cellulomonas flavigena, 10 parts of streptococcus thermophilus, 8 parts of alcaligenes faecalis, 5 parts of bacillus amyloliquefaciens, 2 parts of nitrosomonas sobolifera, 1 part of issatchenkia orientalis and 3 parts of streptomyces bovis; and (5) standby.
Example twoPreparation of microbial agent II
Fully mixing the following component bacteria powder in parts by weight, namely 10 parts of clostridium acetobutylicum, 12 parts of bacillus licheniformis, 20 parts of bacillus subtilis, 5 parts of lactobacillus acidophilus, 7 parts of methane sarcina, 3 parts of methanobacteria mane, 8 parts of rhodopseudomonas palustris, 6 parts of cellulomonas flavigena, 8 parts of streptococcus thermophilus, 10 parts of alcaligenes faecalis, 3 parts of bacillus amyloliquefaciens, 4 parts of nitrosomonas sobria, 3 parts of issatchenkia orientalis and 5 parts of streptomyces bullatae; and (5) standby.
EXAMPLE IIIPreparation of microbial agent
The method comprises the following steps of fully mixing the following component bacteria powder in parts by weight, 9 parts of clostridium acetobutylicum, 14 parts of bacillus licheniformis, 18 parts of bacillus subtilis, 6 parts of lactobacillus acidophilus, 6 parts of methane sarcina, 4 parts of methanobacteria mane, 7 parts of rhodopseudomonas palustris, 5 parts of cellulomonas flavigena, 9 parts of streptococcus thermophilus, 9 parts of alcaligenes faecalis, 4 parts of bacillus amyloliquefaciens, 3 parts of nitrosomonas campestris, 2 parts of issatchenkia orientalis and 4 parts of streptomyces bovis; and (5) standby.
Example fourPreparation of microbial Carriers
a. Preparing organic montmorillonite: weighing 20g of montmorillonite to prepare a suspension with the mass fraction of 10%, adding the suspension into a three-neck flask, adjusting the pH value to 6.0-6.5 by using 0.2mol/L hydrochloric acid, starting heating and stirring, weighing 10g of hexadecyl trimethyl ammonium bromide, dissolving the hexadecyl trimethyl ammonium bromide in water, slightly heating to completely dissolve the hexadecyl trimethyl ammonium bromide, pouring the solution into a dropping funnel, dropwise adding the solution into the three-neck flask, heating and stabilizing the solution for a period of time (80 ℃ for reaction for 20 hours), cooling the solution to room temperature, performing centrifugal separation, performing suction filtration, and washing the solution for 3 times by using deionized water and a 50% ethanol-water solution (volume fraction); crushing the obtained product in a vacuum drying oven at 60 ℃ for 12h by using a crusher, and sieving the crushed product by using a 120-mesh sieve to obtain organic montmorillonite;
b. preparation of a crude carrier: weighing 2g curdlan, adding into a three-neck flask, adding 8g acrylic acid into a 50ml small beaker by using a pipette, neutralizing the acrylic acid to a certain neutralization degree (70%) by using 30% NaOH solution, adding into the three-neck flask, and adding 0.03g (NH)4)2S2O8(initiator), 0.002g N, N' -methylene bisacrylamide (cross-linking agent) is respectively dissolved in 10ml of distilled water and added into three dropping funnels, 1/3 of initiator solution is firstly dropped, the initiator solution is stirred for 10min and then begins to be dropped, the dropping speed of the initiator solution is controlled (dropping is finished within 2 h), the cross-linking agent solution is dropped after 1h (dropping is finished within 1 h), 0.2g of organic montmorillonite is added after dropping is finished, the reaction is continued for 2h and then is finished, the product is washed for 3 times by 1:4 (volume ratio) water-methanol solution and then is dischargedPutting into a vacuum drying oven at 60 ℃ for 12h, and then crushing by a crusher for later use;
c. preparing a carrier finished product: packing the crushed product with filter paper, putting the packed product into a Soxhlet extractor, firstly extracting for 12h by using methanol as a solvent, then extracting for 12h by using acetone as a solvent to remove small molecular substances such as acrylic acid homopolymer, residual monomers and the like, repeatedly washing the extracted residue with distilled water to obtain a purified porous carrier finished product, and crushing the carrier finished product to a certain particle size for later use by a crusher after 12h in a vacuum drying oven at 60 ℃.
Through detection, the porosity of the prepared carrier is 85-90%.
EXAMPLE fiveExperiment for treating sewage
Selecting a sewage discharge river channel with a living area and a chemical plant area at the upstream, firstly arranging a filter screen at the upstream of the river channel to filter sewage, then arranging a treatment point in the river channel behind the filter screen at intervals of 100 meters, totally arranging 10 treatment points, arranging a net which traverses the whole river channel at each treatment point, wherein the net width is 10 meters, and the microbial carrier prepared in the fourth embodiment mixed with the microbial inoculum in the first embodiment is filled in the river channel, the carrier is relatively dense to fill (the filling amount is 30 percent of the volume fraction of the porous carrier in each cubic meter of the effective volume), and the using amount of the microbial agent is increased or decreased according to the pollution degree of the sewage.
The sewage at the upper and lower reaches of the experimental section treated by one month and three months respectively is tested, and the water quality index of the water body is greatly reduced, the color becomes light, the odor is greatly reduced, the sediment amount of the sludge at the bottom of the river channel is reduced, and table 1 shows each experimental data.
TABLE 1 comparison of data before and after wastewater treatment
EXAMPLE sixExperiment 2 of sewage treatment
Selecting a sewage discharge river channel with a living area and a chemical plant area at the upstream, firstly arranging a filter screen at the upstream of the river channel to filter sewage, then arranging a treatment point in the river channel behind the filter screen at intervals of 100 meters, totally arranging 10 treatment points, arranging a net which traverses the whole river channel at each treatment point, wherein the net width is 10 meters, and the microbial carrier prepared in the second embodiment is filled with the microbial inoculum prepared in the fourth embodiment, the carrier is relatively dense in filling (the filling amount is 30 percent of the volume fraction of the porous carrier in each cubic meter of the effective volume), and the using amount of the microbial inoculum is increased or decreased according to the pollution degree of the sewage.
The sewage at the upper and lower reaches of the experimental section treated by one month and three months respectively is tested, and the water quality index of the water body is greatly reduced, the color becomes light, the odor is greatly reduced, the sediment amount of the sludge at the bottom of the river channel is reduced, and table 2 shows each experimental data.
TABLE 2 comparison of data before and after wastewater treatment
EXAMPLE sevenExperiment III for wastewater treatment
Selecting a sewage discharge river channel with a living area and a chemical plant area at the upstream, firstly arranging a filter screen at the upstream of the river channel to filter sewage, then arranging a treatment point in the river channel behind the filter screen at intervals of 100 meters, totally arranging 10 treatment points, arranging a net which traverses the whole river channel at each treatment point, wherein the net width is 10 meters, and the microbial carrier prepared in the fourth embodiment and mixed with the microbial inoculum in the third embodiment is filled in the river channel, wherein the carrier is relatively dense in filling amount (the filling amount is 30 percent of the volume fraction of the porous carrier in each cubic meter of the effective volume), and the using amount of the microbial agent is increased and decreased according to the pollution degree of the sewage.
The sewage at the upper and lower reaches of the experimental section treated by one month and three months respectively is tested, and the water quality index of the water body is found to be greatly reduced, the color becomes light and the odor is greatly reduced, and table 3 shows each experimental data.
TABLE 3 comparison of data before and after wastewater treatment
The embodiment can obtain that the invention carries out sewage treatment by attaching a certain combination of microbial agents to the porous microbial carrier, the water quality index after treatment is greatly reduced, the color is lightened, the odor is greatly reduced, the deposition amount of bottom sludge is greatly reduced, the treatment effect is obvious, and the method is simple and is worthy of popularization and use.
It should be understood that these examples are for illustrative purposes only and are not intended to limit the scope of the present invention. Further, it should be understood that various changes or modifications of the present invention may be made by those skilled in the art after reading the teaching of the present invention, and such equivalents may fall within the scope of the present invention as defined in the appended claims.
Claims (1)
1. The microbial agent for sludge reduction is characterized by comprising the following components in parts by weight: 9 parts of clostridium acetobutylicum, 14 parts of bacillus licheniformis, 18 parts of bacillus subtilis, 6 parts of lactobacillus acidophilus, 6 parts of methane sarcina, 4 parts of mane methane bacteria, 7 parts of rhodopseudomonas palustris, 5 parts of cellulomonas flavigena, 9 parts of streptococcus thermophilus, 9 parts of alcaligenes faecalis, 4 parts of bacillus amyloliquefaciens, 3 parts of nitrosomonas nitrosum, 2 parts of issatchenkia orientalis and 4 parts of streptomyces bullatae;
the preparation method of the carrier of the microbial agent for sludge reduction comprises the following steps:
a. preparing organic montmorillonite: weighing 20g of montmorillonite to prepare a suspension with the mass fraction of 10%, adding the suspension into a three-neck flask, adjusting the pH value to 6.0-6.5 by using 0.2mol/L hydrochloric acid, starting heating and stirring, weighing 10g of hexadecyl trimethyl ammonium bromide, dissolving the hexadecyl trimethyl ammonium bromide in water, slightly heating to completely dissolve the hexadecyl trimethyl ammonium bromide, pouring the solution into a dropping funnel, dropwise adding the solution into the three-neck flask, heating to 80 ℃, reacting for 20 hours, cooling to room temperature, performing centrifugal separation, performing suction filtration, and washing for 3 times by using deionized water and an ethanol-water solution with the volume fraction of 50%; crushing the obtained product in a vacuum drying oven at 60 ℃ for 12h by using a crusher, and sieving the crushed product by using a 120-mesh sieve to obtain organic montmorillonite;
b. preparation of a crude carrier: weighing 2g curdlan, adding into a three-neck flask, and adding 8g acrylic acid into 50m with a pipettel in a small beaker, acrylic acid was neutralized with 30% NaOH solution to a degree of neutralization of 70%, and added to a three-necked flask, and 0.03g of an initiator (NH)4)2S2O8 0.002g of cross-linking agent N, N-methylene bisacrylamide is respectively dissolved in 10ml of distilled water and added into three dropping funnels, 1/3 of initiator solution is firstly dropped, the initiator solution is started to be dropped after stirring for 10min, the dropping speed is controlled to be 2h, the cross-linking agent solution is started to be dropped after 1h, the dropping speed is controlled to be 1h, 0.2g of organic montmorillonite is added after the dropping is finished, and the reaction is finished after 2 h; washing the product with a water-methanol solution with a volume ratio of 1:4 for 3 times, putting the product into a vacuum drying oven with the temperature of 60 ℃ for 12 hours, and crushing the product with a crusher for later use;
c. preparing a carrier finished product: wrapping the crushed standby product with filter paper, putting the wrapped product into a Soxhlet extractor, firstly extracting for 12 hours by using methanol as a solvent, then extracting for 12 hours by using acetone as a solvent to remove acrylic acid homopolymer and residual monomer micromolecule substances, repeatedly washing the extracted residues with distilled water to obtain a purified porous carrier finished product, and crushing the carrier finished product to a certain particle size for standby by using a crusher after 12 hours in a vacuum drying oven at 60 ℃;
through detection, the porosity of the prepared carrier is 85-90%.
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| CN116854325B (en) * | 2023-09-04 | 2023-11-28 | 山东创业环保科技发展有限公司 | Treatment method of sludge to be incinerated |
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