CN109393490A - The chromium-rich nutritious spirulina powder and preparation method of the extract containing natural flavone - Google Patents
The chromium-rich nutritious spirulina powder and preparation method of the extract containing natural flavone Download PDFInfo
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- CN109393490A CN109393490A CN201811336825.5A CN201811336825A CN109393490A CN 109393490 A CN109393490 A CN 109393490A CN 201811336825 A CN201811336825 A CN 201811336825A CN 109393490 A CN109393490 A CN 109393490A
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- 239000011573 trace mineral Substances 0.000 description 1
- 235000013619 trace mineral Nutrition 0.000 description 1
- 230000014616 translation Effects 0.000 description 1
- 235000019155 vitamin A Nutrition 0.000 description 1
- 239000011719 vitamin A Substances 0.000 description 1
- 229940045997 vitamin a Drugs 0.000 description 1
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
- A23L33/10—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
- A23L33/135—Bacteria or derivatives thereof, e.g. probiotics
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
- A23L33/10—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
- A23L33/105—Plant extracts, their artificial duplicates or their derivatives
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
- A23L33/10—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
- A23L33/14—Yeasts or derivatives thereof
- A23L33/145—Extracts
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
- A23L33/10—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
- A23L33/17—Amino acids, peptides or proteins
- A23L33/185—Vegetable proteins
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- A—HUMAN NECESSITIES
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- A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
- A23L33/10—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
- A23L33/17—Amino acids, peptides or proteins
- A23L33/19—Dairy proteins
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2002/00—Food compositions, function of food ingredients or processes for food or foodstuffs
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Abstract
The present invention relates to chromium-rich nutritious spirulina powder of the extract containing natural flavone and preparation method thereof, it forms as follows: 60-80 parts of spirulina extract, 20-30 parts of whey protein powder, 20-30 parts of hydrolyzed wheat protein matter powder, 10-15 parts of wolfberry fruit extract, 2-6 parts of sophora bud standard sclerutin, 6-10 parts of Cr-enriched yeast extract；The extracting method of the sophora bud standard sclerutin is as follows: S1. raw material smashes: sophora bud rutin raw material drying to moisture content being lower than 8%, is crushed, 60-80 mesh is crossed；S2. aqueous two-phase extracting solution is prepared: the weight percent composition of the aqueous two-phase extracting solution is as follows: 15%-20%N- methylimidazole, dipotassium hydrogen phosphate 5-8%, potassium carbonate 3-6%, surplus are water；S3. it extracts: the smashed raw material powder of step S1 is mixed at 40-60 DEG C with the prepared extracting solution of step S2, stir 0.5-1h, stand 30-45min, layering；S4. it separates: taking upper organic phase to be concentrated, precipitate crystal as sophora bud standard sclerutin.Nutritive value of the present invention is high, has great healthy nutritive value.
Description
Technical field
The present invention relates to chromium-rich nutritious spirulina powder of the extract containing natural flavone and preparation method thereof, belong to nutrition and health care
Product processing technique field.
Background technique
Spirulina is a kind of low photosynthetic autotrophs biology, the filamentous that they are made of unicellular or many cells, in thin
Loose or closely well-regulated spiral rotation shape bending, so gain the name.Spirulina is rich in multiple proteins, multivitamin, mineral
Matter and various bioactivators, it is considered to be the ideal food of human future.Spirulina contain multiple biological activities at
Point: wherein beta carotene, chlorophyll, lutein, polysaccharides and phycocyanin etc. have many unique biochemical properties and life
Manage function.
Beta carotene is the important sources of vitamin A, has anti-oxidant, antitumor, raising immunity of organisms isoreactivity.
Lutein has the function of anti-oxidant and protection retina, referred to as " plant gold ".Phycocyanin is that one kind is prevalent in
Biliproteins in Cells of Blue-green Algae can efficiently capture luminous energy, be a kind of special natural pigment albumen, be in dark blue powder
Shape has water solubility.Phycocyanin can be used as natural pigment additive applied to foods and cosmetics.
Flavone compound is a kind of important natural organic-compound, is that plant generates during long-term natural selection
A kind of secondary metabolite, it is widely present in higher plant and filiciform, stem, leaf, flower, fruit etc., is planting
Most of form for being combined into glycoside or carbon glycosyl with sugar exists in object, and also some exists in a free form.Flavonoid
The pharmaceutical value of object is very high, and the brittleness that can reduce blood vessel, reduces blood lipid and cholesterol at the permeability for improving blood vessel, can effectively prevent
Control the diseases such as hypertension, cerebral hemorrhage, coronary heart diseases and angina pectoris.Many flavones ingredients have cough-relieving, eliminating the phlegm, relieving asthma and antibacterial
Effect, while there is diseases and the free radical resisting oxidations such as protect liver, treatment acute, chronic hepatitis, cirrhosis.
Chromium is the essential trace elements of the human body, participates in the glycometabolism and lipid metaboli process of human body, promotes fat and protein
Synthesis, and then promote growth in humans development.Human body is extremely low to the absorption rate of inorganic chromium, and less than 1%, human body is to Organic Chromium
Utilization rate up to 10-25%.Research shows that: when human body lacks chromium, it is easy to cause diabetes, cardiovascular disease, hyperlipidemia and gallbladder
Sterol etc., in addition there are studies have shown that myopia is also lacked with human body, chromium is related, and when human body lacks chromium, insulin action reduction makes
Obstacle occurs for utilizing for sugar, so that cholesterol level increases, artery sclerosis-diabetes synthesis occurs and lacks chromium disease.
The product of spirulina producer, the country is mainly based on spirulina powder at present, deep processing for spirulina and and its
The deep processed product that its functional raw material compounds (such as natural flavonoid compound and Cr-enriched yeast extract) is less, purport of the present invention
Solving this technical problem.
Summary of the invention
It is an object of the present invention to solving the deficiencies in the prior art, the chromium-rich spiral of the extract containing natural flavone is provided
Algae nutrient powder, the instant powder are compounded with natural flavone extract, and nutritive value is high, in good taste, have great nutrition and health care valence
Value.
The second object of the present invention is to provide the preparation method of the chromium-rich nutritious spirulina powder of the extract containing natural flavone.
The technical solution adopted by the present invention to solve the technical problems is:
The chromium-rich nutritious spirulina powder of the extract containing natural flavone, the spirulina instant powder by following parts by weight Raw material processing
Form: 60-80 parts of spirulina extract, 20-30 parts of whey protein powder, 20-30 parts of hydrolyzed wheat protein matter powder, fructus lycii is extracted
10-15 parts of object, 2-6 parts of sophora bud standard sclerutin, 6-10 parts of Cr-enriched yeast extract；
The extracting method of the sophora bud standard sclerutin is as follows:
S1 raw material smashes: sophora bud rutin raw material drying to moisture content being lower than 8%, is crushed, 60-80 mesh is crossed；
S2 aqueous two-phase extracting solution is prepared: the weight percent composition of the aqueous two-phase extracting solution is as follows: 15%-20%N- methyl miaow
Azoles, dipotassium hydrogen phosphate 5-8%, potassium carbonate 3-6%, surplus are water；
S3 is extracted: the smashed raw material powder of step S1 being mixed at 40-60 DEG C with the prepared extracting solution of step S2, is stirred
0.5-1h is mixed, 30-45min, layering are stood；
S4 separation: it takes upper organic phase to be concentrated, precipitates crystal as sophora bud standard sclerutin.
The medicines and health protection value of flavone compound is very high, and the brittleness that can reduce blood vessel, reduces the permeability for improving blood vessel
Blood lipid and cholesterol can effectively prevent the diseases such as hypertension, cerebral hemorrhage, coronary heart diseases and angina pectoris.In addition, many flavones ingredients
Have the function of cough-relieving, eliminating the phlegm, relieving asthma and antibacterial, while there are the diseases such as protect liver, treatment acute, chronic hepatitis, cirrhosis, and anti-
Free-radical oxidation effect.
The method of common extracting flavonoids has the methods of alcohol extracting method, microwave loss mechanisms, supercritical extraction technique, membrane separation process at present,
But in the actual production process have step is cumbersome, energy consumption is high, yield is lower, solvent usage amount is big, solvent volatilization, extraction when
Between it is longer, can not cope with that flavones is heated easily to be decomposed, the weak disadvantage of stability.
The present invention with inorganic salts replace high polymer common organic matter double-aqueous phase system, suitable for polar substances extraction and
Purifying, split-phase is rapid, and the split-phase time is short, and extraction time is short, and extraction efficiency is high, and N- methylimidazole is conducive to flavonoids in alkalinity
Substance is selectively allocated in upper phase in double-aqueous phase system, and extraction efficiency is high, and purification effect is good.In addition, carrying out at normal temperature, item
Part is mild, and product purity is higher, high income.
Preferably, the spirulina instant powder is formed by the Raw material processing of following parts by weight: 70 parts of spirulina extract, cream
25 parts of albumin matter powder, 25 parts of hydrolyzed wheat protein matter powder, 12 parts of wolfberry fruit extract, 4 parts of sophora bud standard sclerutin, Cr-enriched yeast mentions
Take 8 parts of object；
The extracting method of the sophora bud standard sclerutin is as follows:
S1 raw material smashes: sophora bud rutin raw material drying to moisture content being lower than 8%, is crushed, 60 meshes are crossed；
S2 aqueous two-phase extracting solution is prepared: the weight percent composition of the aqueous two-phase extracting solution is as follows: 18%N- methylimidazole, phosphorus
Sour hydrogen dipotassium 6%, potassium carbonate 5%, surplus are water；
S3 is extracted: the smashed raw material powder of step S1 being mixed at 50 DEG C with the prepared extracting solution of step S2, is stirred
0.6h stands 45min, layering；
S4 separation: it takes upper organic phase to be concentrated, precipitates crystal as sophora bud standard sclerutin.
Preferably, the mass ratio of raw material powder and the double-aqueous phase system is 1:9-1:12 in the step S3.
Preferably, the spirulina extract the preparation method is as follows:
S1: by the spirulina of fresh picking, after cleaning up, organization protection's liquid is infiltrated, freezes 1-3h preservation, the organization protection
The weight percent of liquid forms are as follows: the oxidized dextran of 2.0-5.0%, antioxidant 1.0-2.0%, bacteriostatic agent 0.5-0.8%；
Oxidized dextran is a kind of macromolecule glucose polymer that natural glucan obtains after sodium periodate oxidation, contains more aldehyde
Base has good stable cross-linking properties, and biocompatibility is fabulous, with other fibre fractionations can be fabulous it is compatible.The application
Inventor practice in find, multiple aldehyde radicals that oxidized dextran contains can be with high reaction activity exposed in spirulina crude fibre
Group carry out cross-linking reaction, play the role of intermolecular bridge formation dispersing agent, improve the stability and enzymatic hydrolysis of subsequent enzymatic hydrolysis stoste
Effect improves the quality of product.
S2: enzymatic hydrolysis stoste is purchased: by the spirulina of step S1 freezing in 60-70 DEG C of hot water, being sufficiently stirred mixed
Even to form stable enzymatic hydrolysis stoste, enzymatic hydrolysis stoste temperature is adjusted to 40-50 DEG C, and pH is adjusted to 6.5-7.2；Utilize the method for freeze thawing
Broken wall；
S3: the complex enzyme zymohydrolysis 45-60min of spirulina raw material weight in wet base 2-5% is added into the enzymatic hydrolysis stoste of step S2, digested
Maintain pH constant in journey, complex enzyme is the mixture of bromelain or papain and neutral cellulase, and enzymatic hydrolysis is completed
Enzyme deactivation, centrifugation obtain centrifugal clear liquid；
S4: the centrifugal clear liquid of step S3, using ultrafiltration membrane ultrafiltration, permeate is concentrated, is spray-dried, the ultrafiltration membrane
Molecular cut off is 3000-4500Da.
Preferably, the specific rotatory power of the oxidized dextran is 180-185, average molecular weight 4100-4200, oxidizability
For 130-135%, the antioxidant is reduced glutathione or ascorbic acid, and the bacteriostatic agent is bee glue powder, and tissue is protected
The synergistic effect for protecting liquid avoids enzymolysis process enzymolysis liquid rotten, and breeds bacterium；
Preferably, the step S3 complex enzyme is by papain or bromelain and neutral cellulase 6-8 in mass ratio:
The enzyme activity of 1 composition, complex enzyme is not less than 2000U/g.
Preferably, the Cr-enriched yeast extract the preparation method is as follows:
S1: being collected by centrifugation the Cr-enriched yeast that fermentation obtains, and infiltrates organization protection's liquid, freezes 1-3h preservation, organization protection's liquid
Weight percent composition are as follows: the oxidized dextran of 2.0-5.0%, antioxidant 1.0-2.0%, bacteriostatic agent 0.5-0.8%；
S2: enzymatic hydrolysis stoste is purchased: by the Cr-enriched yeast of step S1 freezing in 70-80 DEG C of hot water, mixing is sufficiently stirred,
Stable enzymatic hydrolysis stoste is formed after homogeneous, enzymatic hydrolysis stoste temperature is adjusted to 45-55 DEG C, and pH is adjusted to 6.5-7.5；
S3: the complex enzyme zymohydrolysis 45-60min of Cr-enriched yeast raw material weight in wet base 2-5%, enzymatic hydrolysis are added into the enzymatic hydrolysis stoste of step S2
Maintain pH constant in the process, complex enzyme is the mixture of bromelain or papain and 1,4 beta-glucanase, and enzymatic hydrolysis is completed
Enzyme deactivation, centrifugation obtain centrifugal clear liquid；
S4: the centrifugal clear liquid of step S3, using ultrafiltration membrane ultrafiltration, permeate is concentrated, is spray-dried, the ultrafiltration membrane
Molecular cut off is 4000-6000Da.
Preferably, in the step S3 complex enzyme by bromelain or papain and 1,4 beta-glucanase in mass ratio
The enzyme activity of 8-10:1 composition, complex enzyme is not less than 2500U/g.
Preferably, the weight percent composition of organization protection's liquid are as follows: 2.0% oxidized dextran, antioxidant
1.5%, bacteriostatic agent 0.6%.
The preparation method of the chromium-rich nutritious spirulina powder of the extract containing natural flavone, it is described the preparation method is as follows: will formula
The spirulina extract of amount, whey protein powder, hydrolyzed wheat protein matter powder, wolfberry fruit extract, sophora bud standard sclerutin and chromium-rich
Yeast extract is uniformly mixed, and crosses 80-100 mesh, Vacuum Package.
The beneficial effects of the present invention are: spirulina and sophora bud rutin are carried out deep processing by the present invention, spiral is made respectively
Algae extract, Cr-enriched yeast extract and sophora bud standard sclerutin, wherein flavones content is high in sophora bud rutin, then with other components
Spirulina instant powder is made in compounding, and nutritive value is high, in good taste, has great healthy nutritive value.
In addition, the present invention replaces the common organic matter double-aqueous phase system of high polymer with inorganic salts, suitable for polar substances
It extracts and purifies, split-phase is rapid, and the split-phase time is short, and extraction time is short, and extraction efficiency is high, and N- methylimidazole is conducive in alkalinity
Flavonoid substances are selectively allocated in upper phase in double-aqueous phase system, and extraction efficiency is high, and purification effect is good.In addition, at normal temperature
It carries out, mild condition, product purity is higher, high income.
Specific embodiment
Below by specific embodiment, technical scheme of the present invention will be further explained in detail.
Embodiment 1:
The chromium-rich nutritious spirulina powder of the extract containing natural flavone, the chromium-rich nutritious spirulina powder of the extract containing natural flavone
It is formed by the Raw material processing of following parts by weight: 60 parts of spirulina extract, 20 parts of whey protein powder, hydrolyzed wheat protein matter powder
20 parts, 10 parts of wolfberry fruit extract, 2 parts of sophora bud standard sclerutin, 6 parts of Cr-enriched yeast extract；
The extracting method of the sophora bud standard sclerutin is as follows:
S1 raw material smashes: sophora bud rutin raw material drying to moisture content being lower than 8%, is crushed, 60 meshes are crossed；
S2 aqueous two-phase extracting solution is prepared: the weight percent composition of the aqueous two-phase extracting solution is as follows: N- methylimidazole 15%, phosphorus
Sour hydrogen dipotassium 5%, potassium carbonate 3%, surplus are water；
S3 is extracted: the smashed raw material powder of step S1 being mixed at 40 DEG C with the prepared extracting solution of step S2, is stirred
1h stands 45min, layering；
S4 separation: it takes upper organic phase to be concentrated, precipitates crystal as sophora bud standard sclerutin；
The mass ratio of raw material powder and the double-aqueous phase system is 1:9 in the step S3；
The spirulina extract the preparation method is as follows:
S1: by the spirulina of fresh picking, after cleaning up, organization protection's liquid is infiltrated, freezes 1h preservation, organization protection's liquid
Weight percent composition are as follows: 2.0% oxidized dextran, antioxidant 1.0%, bacteriostatic agent 0.5%；
S2: enzymatic hydrolysis stoste is purchased: by the spirulina of step S1 freezing in 60 DEG C of hot water, mixing is sufficiently stirred and is formed surely
Fixed enzymatic hydrolysis stoste, enzymatic hydrolysis stoste temperature are adjusted to 40 DEG C, and pH is adjusted to 6.5；Utilize the method broken wall of freeze thawing；
S3: the complex enzyme zymohydrolysis 45min of spirulina raw material weight in wet base 2% is added into the enzymatic hydrolysis stoste of step S2, is tieed up in enzymolysis process
Hold that pH is constant, complex enzyme is the mixture of bromelain and neutral cellulase, and enzymatic hydrolysis completes enzyme deactivation, and centrifugation is centrifuged
Clear liquid；
S4: the centrifugal clear liquid of step S3, using ultrafiltration membrane ultrafiltration, permeate is concentrated, is spray-dried, the ultrafiltration membrane
Molecular cut off is 3000Da.
The specific rotatory power of the oxidized dextran is 180-185, average molecular weight 4100-4200, oxidizability 130-
135%, the antioxidant is reduced glutathione or ascorbic acid, and the bacteriostatic agent is bee glue powder, organization protection's liquid
Synergistic effect avoids enzymolysis process enzymolysis liquid rotten, and breeds bacterium；The step S3 complex enzyme is by papain or spinach
Trailing plants protease and neutral cellulase 6:1 in mass ratio are formed, and the enzyme activity of complex enzyme is not less than 2000U/g.
The Cr-enriched yeast extract the preparation method is as follows:
S1: being collected by centrifugation the Cr-enriched yeast that fermentation obtains, and infiltrates organization protection's liquid, freezes 1h preservation, organization protection's liquid
Weight percent composition are as follows: 2.0% oxidized dextran, antioxidant 1.0%, bacteriostatic agent 0.5%；
S2: enzymatic hydrolysis stoste is purchased: by the Cr-enriched yeast of step S1 freezing in 70 DEG C of hot water, mixing is sufficiently stirred,
Stable enzymatic hydrolysis stoste is formed after matter, enzymatic hydrolysis stoste temperature is adjusted to 45 DEG C, and pH is adjusted to 6.5；
S3: the complex enzyme zymohydrolysis 45-60min of Cr-enriched yeast raw material weight in wet base 2% is added into the enzymatic hydrolysis stoste of step S2, digested
Maintain pH constant in journey, complex enzyme is the mixture of papain and 1,4 beta-glucanase, and enzymatic hydrolysis completes enzyme deactivation, is centrifuged, and is obtained
Centrifugal clear liquid；
S4: the centrifugal clear liquid of step S3, using ultrafiltration membrane ultrafiltration, permeate is concentrated, is spray-dried, the ultrafiltration membrane
Molecular cut off is 4000-6000Da.
Complex enzyme is by bromelain or papain and 1,4 beta-glucanase 8:1 group in mass ratio in the step S3
At the enzyme activity of complex enzyme is not less than 2500U/g.
By the spirulina extract of formula ratio, whey protein powder, hydrolyzed wheat protein matter powder, wolfberry fruit extract, the sophora bud
Standard sclerutin and Cr-enriched yeast extract are uniformly mixed, and cross 80 meshes, Vacuum Package.
Embodiment 2
The chromium-rich nutritious spirulina powder of the extract containing natural flavone, the chromium-rich nutritious spirulina powder of the extract containing natural flavone
It is formed by the Raw material processing of following parts by weight: 80 parts of spirulina extract, 30 parts of whey protein powder, hydrolyzed wheat protein matter powder
30 parts, 15 parts of wolfberry fruit extract, 6 parts of sophora bud standard sclerutin, 10 parts of Cr-enriched yeast extract；
The extracting method of the sophora bud standard sclerutin is as follows:
S1 raw material smashes: sophora bud rutin raw material drying to moisture content being lower than 8%, is crushed, 80 meshes are crossed；
S2 aqueous two-phase extracting solution is prepared: the weight percent composition of the aqueous two-phase extracting solution is as follows: N- methylimidazole 20%, phosphorus
Sour hydrogen dipotassium 8%, potassium carbonate 6%, surplus are water；
S3 is extracted: the smashed raw material powder of step S1 being mixed at 60 DEG C with the prepared extracting solution of step S2, is stirred
0.5h stands 30min, layering；
S4 separation: it takes upper organic phase to be concentrated, precipitates crystal as sophora bud standard sclerutin.
The mass ratio of raw material powder and the double-aqueous phase system is 1:12 in the step S3；
The spirulina extract the preparation method is as follows:
S1: by the spirulina of fresh picking, after cleaning up, organization protection's liquid is infiltrated, freezes 3h preservation, organization protection's liquid
Weight percent composition are as follows: 5.0% oxidized dextran, antioxidant 2.0%, bacteriostatic agent 0.8%；
S2: enzymatic hydrolysis stoste is purchased: by the spirulina of step S1 freezing in 70 DEG C of hot water, mixing is sufficiently stirred and is formed surely
Fixed enzymatic hydrolysis stoste, enzymatic hydrolysis stoste temperature are adjusted to 50 DEG C, and pH is adjusted to 7.2；Utilize the method broken wall of freeze thawing；
S3: the complex enzyme zymohydrolysis 60min of spirulina raw material weight in wet base 5% is added into the enzymatic hydrolysis stoste of step S2, is tieed up in enzymolysis process
Hold that pH is constant, complex enzyme is the mixture of papain and neutral cellulase, and enzymatic hydrolysis completes enzyme deactivation, and centrifugation is centrifuged
Clear liquid；
S4: the centrifugal clear liquid of step S3, using ultrafiltration membrane ultrafiltration, permeate is concentrated, is spray-dried, the ultrafiltration membrane
Molecular cut off is 4500Da.
The specific rotatory power of the oxidized dextran is 180-185, average molecular weight 4100-4200, oxidizability 130-
135%, the antioxidant is reduced glutathione or ascorbic acid, and the bacteriostatic agent is bee glue powder, organization protection's liquid
Synergistic effect avoids enzymolysis process enzymolysis liquid rotten, and breeds bacterium；The step S3 complex enzyme is by papain or spinach
Trailing plants protease and neutral cellulase 8:1 in mass ratio are formed, and the enzyme activity of complex enzyme is not less than 2000U/g.
The Cr-enriched yeast extract the preparation method is as follows:
S1: being collected by centrifugation the Cr-enriched yeast that fermentation obtains, and infiltrates organization protection's liquid, freezes 3h preservation, organization protection's liquid
Weight percent composition are as follows: 5.0% oxidized dextran, antioxidant 2.0%, bacteriostatic agent 0.8%；
S2: enzymatic hydrolysis stoste is purchased: by the Cr-enriched yeast of step S1 freezing in 80 DEG C of hot water, mixing is sufficiently stirred,
Stable enzymatic hydrolysis stoste is formed after matter, enzymatic hydrolysis stoste temperature is adjusted to 55 DEG C, and pH is adjusted to 7.5；
S3: the complex enzyme zymohydrolysis 60min of Cr-enriched yeast raw material weight in wet base 5% is added into the enzymatic hydrolysis stoste of step S2, in enzymolysis process
Maintain pH constant, complex enzyme is the mixture of bromelain and 1,4 beta-glucanase, and enzymatic hydrolysis completes enzyme deactivation, and centrifugation is centrifuged
Clear liquid；
S4: the centrifugal clear liquid of step S3, using ultrafiltration membrane ultrafiltration, permeate is concentrated, is spray-dried, the ultrafiltration membrane
Molecular cut off is 6000Da, and complex enzyme presses matter by bromelain or papain and 1,4 beta-glucanase in the step S3
Amount is formed than 10:1, and the enzyme activity of complex enzyme is not less than 2500U/g.
By the spirulina extract of formula ratio, whey protein powder, hydrolyzed wheat protein matter powder, wolfberry fruit extract, the sophora bud
Standard sclerutin and Cr-enriched yeast extract are uniformly mixed, and are sieved with 100 mesh sieve, Vacuum Package.
Embodiment 3
The chromium-rich nutritious spirulina powder of the extract containing natural flavone, the spirulina instant powder by following parts by weight Raw material processing
It forms: 70 parts of spirulina extract, 25 parts of whey protein powder, 25 parts of hydrolyzed wheat protein matter powder, 12 parts of wolfberry fruit extract, Chinese scholartree
4 parts of standard sclerutin, 8 parts of Cr-enriched yeast extract of rice；
The extracting method of the sophora bud standard sclerutin is as follows:
S1 raw material smashes: sophora bud rutin raw material drying to moisture content being lower than 8%, is crushed, 60 meshes are crossed；
S2 aqueous two-phase extracting solution is prepared: the weight percent composition of the aqueous two-phase extracting solution is as follows: 18%N- methylimidazole, phosphorus
Sour hydrogen dipotassium 6%, potassium carbonate 5%, surplus are water；
S3 is extracted: the smashed raw material powder of step S1 being mixed at 50 DEG C with the prepared extracting solution of step S2, is stirred
0.6h stands 45min, layering；
S4 separation: it takes upper organic phase to be concentrated, precipitates crystal as sophora bud standard sclerutin, the Central Plains step S3 feed powder
The last mass ratio with the double-aqueous phase system is 1:10；
The spirulina extract the preparation method is as follows:
S1: by the spirulina of fresh picking, after cleaning up, organization protection's liquid is infiltrated, freezes 2h preservation, organization protection's liquid
Weight percent composition are as follows: 2.0% oxidized dextran, antioxidant 1.5%, bacteriostatic agent 0.6%；
S2: enzymatic hydrolysis stoste is purchased: by the spirulina of step S1 freezing in 65 DEG C of hot water, mixing is sufficiently stirred and is formed surely
Fixed enzymatic hydrolysis stoste, enzymatic hydrolysis stoste temperature are adjusted to 45 DEG C, and pH is adjusted to 7.0；Utilize the method broken wall of freeze thawing；
S3: the complex enzyme zymohydrolysis 50min of spirulina raw material weight in wet base 3% is added into the enzymatic hydrolysis stoste of step S2, is tieed up in enzymolysis process
Hold that pH is constant, complex enzyme is the mixture of bromelain or papain and neutral cellulase, and enzymatic hydrolysis completes enzyme deactivation, from
The heart obtains centrifugal clear liquid；
S4: the centrifugal clear liquid of step S3, using ultrafiltration membrane ultrafiltration, permeate is concentrated, is spray-dried, the ultrafiltration membrane
Molecular cut off is 3600Da.
The specific rotatory power of the oxidized dextran is 180-185, average molecular weight 4100-4200, oxidizability 130-
135%, the antioxidant is reduced glutathione or ascorbic acid, and the bacteriostatic agent is bee glue powder, organization protection's liquid
Synergistic effect avoids enzymolysis process enzymolysis liquid rotten, and breeds bacterium, and the step S3 complex enzyme is by papain in
Property cellulase 7:1 in mass ratio composition, the enzyme activity of complex enzyme is not less than 2000U/g.
The Cr-enriched yeast extract the preparation method is as follows:
S1: being collected by centrifugation the Cr-enriched yeast that fermentation obtains, and infiltrates organization protection's liquid, freezes 2h preservation, organization protection's liquid
Weight percent composition are as follows: 2.0% oxidized dextran, antioxidant 1.5%, bacteriostatic agent 0.6%；
S2: enzymatic hydrolysis stoste is purchased: by the Cr-enriched yeast of step S1 freezing in 75 DEG C of hot water, mixing is sufficiently stirred,
Stable enzymatic hydrolysis stoste is formed after matter, enzymatic hydrolysis stoste temperature is adjusted to 50 DEG C, and pH is adjusted to 7.0；
S3: the complex enzyme zymohydrolysis 50min of Cr-enriched yeast raw material weight in wet base 3% is added into the enzymatic hydrolysis stoste of step S2, in enzymolysis process
Maintain pH constant, complex enzyme is the mixture of bromelain or papain and 1,4 beta-glucanase, and enzymatic hydrolysis completes enzyme deactivation, from
The heart obtains centrifugal clear liquid；
S4: the centrifugal clear liquid of step S3, using ultrafiltration membrane ultrafiltration, permeate is concentrated, is spray-dried, the ultrafiltration membrane
Molecular cut off is 5000Da.
Complex enzyme is made of papain and 1,4 beta-glucanase 9:1 in mass ratio in the step S3, the enzyme of complex enzyme
It is living to be not less than 2500U/g.
It is described the preparation method is as follows: by the spirulina extract of formula ratio, whey protein powder, hydrolyzed wheat protein matter
Powder, wolfberry fruit extract, sophora bud standard sclerutin and Cr-enriched yeast extract are uniformly mixed, and cross 90 meshes, Vacuum Package.
Above-mentioned embodiment is only a preferred solution of the present invention, not the present invention is made in any form
Limitation, there are also other variations and modifications on the premise of not exceeding the technical scheme recorded in the claims.
Claims (10)
1. the chromium-rich nutritious spirulina powder of the extract containing natural flavone, it is characterised in that: the richness of the extract containing natural flavone
Chromium nutritious spirulina powder is formed by the Raw material processing of following parts by weight: 60-80 parts of spirulina extract, whey protein powder 20-
30 parts, 20-30 parts of hydrolyzed wheat protein matter powder, 10-15 parts of wolfberry fruit extract, 2-6 parts of sophora bud standard sclerutin, Cr-enriched yeast extracts
6-10 parts of object；
The extracting method of the sophora bud standard sclerutin is as follows:
S1 raw material smashes: sophora bud raw material drying to moisture content being lower than 8%, is crushed, 60-80 mesh is crossed；
S2 aqueous two-phase extracting solution is prepared: the weight percent composition of the aqueous two-phase extracting solution is as follows: 15%-20%N- methyl miaow
Azoles, dipotassium hydrogen phosphate 5-8%, potassium carbonate 3-6%, surplus are water；
S3 is extracted: the smashed raw material powder of step S1 being mixed at 40-60 DEG C with the prepared extracting solution of step S2, is stirred
0.5-1h is mixed, 30-45min, layering are stood；
S4 separation: it takes upper organic phase to be concentrated, precipitates crystal as sophora bud standard sclerutin.
2. the chromium-rich nutritious spirulina powder of the extract according to claim 1 containing natural flavone, it is characterised in that: described to contain
The chromium-rich nutritious spirulina powder of natural flavone extract is formed by the Raw material processing of following parts by weight: 70 parts of spirulina extract,
25 parts of whey protein powder, 25 parts of hydrolyzed wheat protein matter powder, 12 parts of wolfberry fruit extract, 4 parts of sophora bud standard sclerutin, Cr-enriched yeast
8 parts of extract；
The extracting method of the sophora bud standard sclerutin is as follows:
S1 raw material smashes: sophora bud raw material drying to moisture content being lower than 8%, is crushed, 60 meshes are crossed；
S2 aqueous two-phase extracting solution is prepared: the weight percent composition of the aqueous two-phase extracting solution is as follows: 18%N- methylimidazole, phosphorus
Sour hydrogen dipotassium 6%, potassium carbonate 5%, surplus are water；
S3 is extracted: the smashed raw material powder of step S1 being mixed at 50 DEG C with the prepared extracting solution of step S2, is stirred
0.6h stands 45min, layering；
S4 separation: it takes upper organic phase to be concentrated, precipitates crystal as sophora bud standard sclerutin.
3. the chromium-rich nutritious spirulina powder of the extract according to claim 1 or 2 containing natural flavone, it is characterised in that: institute
Stating the mass ratio of raw material powder and the double-aqueous phase system in step S3 is 1:9-1:12.
4. the chromium-rich nutritious spirulina powder of the extract according to claim 1 containing natural flavone, it is characterised in that:
The spirulina extract the preparation method is as follows:
S1: by the spirulina of fresh picking, after cleaning up, organization protection's liquid is infiltrated, freezes 1-3h preservation, the organization protection
The weight percent of liquid forms are as follows: the oxidized dextran of 2.0-5.0%, antioxidant 1.0-2.0%, bacteriostatic agent 0.5-0.8%；
S2: enzymatic hydrolysis stoste is purchased: by the spirulina of step S1 freezing in 60-70 DEG C of hot water, mixing shape is sufficiently stirred
At stable enzymatic hydrolysis stoste, digests stoste temperature and adjust to 40-50 DEG C, pH is adjusted to 6.5-7.2；
S3: the complex enzyme zymohydrolysis 45-60min of spirulina raw material weight in wet base 2-5% is added into the enzymatic hydrolysis stoste of step S2, digested
Maintain pH constant in journey, complex enzyme is the mixture of bromelain or papain and neutral cellulase, and enzymatic hydrolysis is completed
Enzyme deactivation, centrifugation obtain centrifugal clear liquid；
S4: the centrifugal clear liquid of step S3, using ultrafiltration membrane ultrafiltration, permeate is concentrated, is spray-dried, the ultrafiltration membrane
Molecular cut off is 3000-4500Da.
5. the chromium-rich nutritious spirulina powder of the extract according to claim 4 containing natural flavone, it is characterised in that: the oxygen
The specific rotatory power for changing glucan is 180-185, average molecular weight 4100-4200, oxidizability 130-135%, described anti-oxidant
Agent is reduced glutathione or ascorbic acid, and the bacteriostatic agent is bee glue powder.
6. the chromium-rich nutritious spirulina powder of the extract according to claim 4 containing natural flavone, it is characterised in that: the step
Rapid S3 complex enzyme is made of papain or bromelain with neutral cellulase 6-8:1 in mass ratio, the enzyme of complex enzyme
It is living to be not less than 2000U/g.
7. the chromium-rich nutritious spirulina powder of the extract according to claim 1 containing natural flavone, it is characterised in that:
The Cr-enriched yeast extract the preparation method is as follows:
S1: being collected by centrifugation the Cr-enriched yeast that fermentation obtains, and infiltrates organization protection's liquid, freezes 1-3h preservation, organization protection's liquid
Weight percent composition are as follows: the oxidized dextran of 2.0-5.0%, antioxidant 1.0-2.0%, bacteriostatic agent 0.5-0.8%；
S2: enzymatic hydrolysis stoste is purchased: by the Cr-enriched yeast of step S1 freezing in 70-80 DEG C of hot water, mixing is sufficiently stirred,
Stable enzymatic hydrolysis stoste is formed after homogeneous, enzymatic hydrolysis stoste temperature is adjusted to 45-55 DEG C, and pH is adjusted to 6.5-7.5；
S3: the complex enzyme zymohydrolysis 45-60min of Cr-enriched yeast raw material weight in wet base 2-5%, enzymatic hydrolysis are added into the enzymatic hydrolysis stoste of step S2
Maintain pH constant in the process, complex enzyme is the mixture of bromelain or papain and 1,4 beta-glucanase, and enzymatic hydrolysis is completed
Enzyme deactivation, centrifugation obtain centrifugal clear liquid；
S4: the centrifugal clear liquid of step S3, using ultrafiltration membrane ultrafiltration, permeate is concentrated, is spray-dried, the ultrafiltration membrane
Molecular cut off is 4000-6000Da.
8. the chromium-rich nutritious spirulina powder of the extract according to claim 7 containing natural flavone, it is characterised in that: the step
Complex enzyme is made of bromelain or papain with 1,4 beta-glucanase 8-10:1 in mass ratio in rapid S3, the enzyme of complex enzyme
It is living to be not less than 2500U/g.
9. the chromium-rich nutritious spirulina powder of the extract containing natural flavone according to claim 4 or 7, it is characterised in that: institute
State the weight percent composition of organization protection's liquid are as follows: 2.0% oxidized dextran, antioxidant 1.5%, bacteriostatic agent 0.6%.
10. a kind of preparation of the chromium-rich nutritious spirulina powder of the extract described in any one of claim 1-9 containing natural flavone
Method, which is characterized in that described the preparation method is as follows: the spirulina extract of formula ratio, whey protein powder, wheat are hydrolyzed
Protein powder, wolfberry fruit extract, sophora bud standard sclerutin and Cr-enriched yeast extract are uniformly mixed, and cross 80-100 mesh, vacuum seal
Dress.
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| CN115956675A (en) * | 2022-12-08 | 2023-04-14 | 杭州娃哈哈科技有限公司 | Composition for protecting gastric mucosa, preparation method and application thereof |
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