CN109370952A - Deodorization complex microorganism bacterium solution and its preparation method and application method - Google Patents
Deodorization complex microorganism bacterium solution and its preparation method and application method Download PDFInfo
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- CN109370952A CN109370952A CN201811464186.0A CN201811464186A CN109370952A CN 109370952 A CN109370952 A CN 109370952A CN 201811464186 A CN201811464186 A CN 201811464186A CN 109370952 A CN109370952 A CN 109370952A
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- bacterium solution
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- microorganism bacterium
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/20—Bacteria; Culture media therefor
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- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F11/00—Treatment of sludge; Devices therefor
- C02F11/02—Biological treatment
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- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F2303/00—Specific treatment goals
- C02F2303/02—Odour removal or prevention of malodour
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- Disinfection, Sterilisation Or Deodorisation Of Air (AREA)
Abstract
The present invention provides a kind of deodorization complex microorganism bacterium solution and its preparation method and application method, wherein, the bacterium number accounting of each strain in the deodorization complex microorganism bacterium solution are as follows: lactobacillus plantarum is 20 ~ 30%, liquid starchy bacillus is 20 ~ 30%, bacillus licheniformis is 20 ~ 30%, and bafillus natto is 20 ~ 30%.The present invention is based on provide a kind of deodorization complex microorganism bacterium solution using the symbiosis between bacterial strain, alternate, synergistic effect relationship, it is formed by separation screening efficient deodorizing function strain and optimum organization compatibility, the stench intensity that its release can be effectively reduced is inoculated in water closet septic tank, to reach water closet septic tank stench in-situ control purpose.
Description
Technical field
The present invention relates to a kind of deodorization technology fields more particularly to a kind of deodorization complex microorganism bacterium solution and preparation method thereof
And methods for using them.
Background technique
Odor pollution is universally acknowledged one of seven overall situation public hazards, and continuous with China's modern economy continues health fastly
The development of speed, but either tourist attractions, public place of entertainment, school, hospital, office building, shopping center, factory, residential quarter etc.
Lavatory and its septic tank caused by stench have been to be concerned by more and more people.It is raw especially with expanding economy and people
The flat continuous improvement of running water, the requirement to environmental quality are also increasingly strong.
Excrement odor pollution is not only brought inconvenience to people's life, and has an effect on its quality, wherein containing there are many toxic
Harmful components, wherein ammonia, trimethylamine, hydrogen sulfide, methyl mercaptan, sulfurized alcohol, methyl disulfide etc. are main pernicious gas ingredients,
Very big harm can be brought to people's lives environment, reduce its quality of life, with greater need for being carried out disinfection with chemical agent and
Cleaning, will lead to the use of a large amount of chemical agent in this way.A large amount of chemical noxious substance can also make water body and soil simultaneously
At serious harm, human health and food safety are seriously endangered.
And the discharge of foul smell gas can cause surrounding enviroment seriously to pollute, and people can be to health after sucking for a long time
It causes to seriously endanger.Therefore implement septic tank contamination control and principle, the research for carrying out removing foul gas is of great significance.
Currently, can be divided into two broad aspect of chemical deodorizing and biological deodorizing for stench removing research, chemical deodorizing principle is
Oxidative degradation is carried out to odor pollutant by external source Strong oxdiative substance, and then removes stench, it is with strong points, release is fast, but its
Application range is small, can fail when encountering complex effluvium gas componant, and when use chemical deodorizing agent, remaining chemical substance is past
It is past to cause secondary pollution.Therefore, in view of the above-mentioned problems, it is necessary to propose further solution.
Summary of the invention
The purpose of the present invention is to provide a kind of deodorization complex microorganism bacterium solution and its preparation method and application method, with gram
Take the deficiencies in the prior art.
For achieving the above object, the present invention provides a kind of deodorization complex microorganism bacterium solution, the deodorization composite microbial
The bacterium number accounting of each strain in object bacterium solution are as follows:
Lactobacillus plantarum is 20 ~ 30%, and liquid starchy bacillus is 20 ~ 30%, and bacillus licheniformis is 20 ~ 30%, natto bud
Spore bacillus is 20 ~ 30%.
As the improvement of deodorization complex microorganism bacterium solution of the invention, each strain in the deodorization complex microorganism bacterium solution
Bacterium number accounting are as follows:
Lactobacillus plantarum is 25%, and liquid starchy bacillus is 25%, bacillus licheniformis 25%, and bafillus natto is
25%。
For achieving the above object, the present invention provides a kind of preparation side of deodorization complex microorganism bacterium solution as described above
Method comprising following steps:
S1, lactobacillus plantarum, liquid starchy bacillus, bacillus licheniformis, bafillus natto are divided by bacterium number accounting
It is spare that respective fluid nutrient medium activation is not accessed；
S2, the fluid nutrient medium of complex microorganism bacterium solution is subjected to sterilization treatment and is quantified spare；
S4, the fluid nutrient medium of each strain access complex microorganism bacterium solution after activation is cultivated；
S5, the bacterium solution cultivated in the fluid nutrient medium of complex microorganism bacterium solution is detected, when bacterium solution viable count be greater than etc.
It is less than or equal to 4 in 100,000,000 CFU/ml, pH, when the color of suspension is blush, obtains deodorization complex microorganism bacterium solution of the invention.
As the improvement of preparation method of the invention, the fluid nutrient medium of the complex microorganism bacterium solution is by mass percentage
Including following component:
4 ~ 6g of peptone, 12 ~ 18g of starch, 80 ~ 130g of brown sugar, 4 ~ 6g of agar, 4 ~ 6g of sodium chloride, 800 ~ 1200ml of water.
As the improvement of preparation method of the invention, the fluid nutrient medium of the complex microorganism bacterium solution is by mass percentage
Including following component: peptone 5g, starch 15g, brown sugar 100g, agar 5g, sodium chloride 5g, water 1000ml.
As the improvement of preparation method of the invention, the pH of the fluid nutrient medium of the complex microorganism bacterium solution is 6 ~ 7.
For achieving the above object, the present invention provides a kind of application side of deodorization complex microorganism bacterium solution as described above
Method comprising following steps:
Deodorization complex microorganism bacterium solution is sprayed into solarization in urinal or septic tank in proportion and repeatedly, while being ensured between adjacent time
With suitable time interval, the use allotment ratio for the deodorization complex microorganism bacterium solution sprayed are as follows: bacterium: water=2:100.
Compared with prior art, the beneficial effects of the present invention are: the present invention is based on using between bacterial strain symbiosis, alternate,
Synergistic effect relationship provides a kind of deodorization complex microorganism bacterium solution, passes through separation screening efficient deodorizing function strain and optimization group
It closes compatibility to form, the stench intensity of its release can be effectively reduced by being inoculated in water closet septic tank, to reach lavatory
Manure pit stench in-situ control purpose.
Specific embodiment
The present invention is described in detail combined with specific embodiments below, but it should be stated that, these embodiments are simultaneously
Non- limitation of the present invention, those of ordinary skill in the art are according to these embodiments in made function, method or structure
Equivalent transformation or substitution, all belong to the scope of protection of the present invention within.
The present invention provides a kind of deodorization complex microorganism bacterium solution, the bacterium number of each strain in the deodorization complex microorganism bacterium solution
Accounting are as follows:
Lactobacillus plantarum is 20 ~ 30%, and liquid starchy bacillus is 20 ~ 30%, and bacillus licheniformis is 20 ~ 30%, natto bud
Spore bacillus is 20 ~ 30%.Preferably, in the deodorization complex microorganism bacterium solution each strain bacterium number accounting are as follows: lactobacillus plantarum
It is 25%, liquid starchy bacillus is 25%, bacillus licheniformis 25%, bafillus natto 25%.
Wherein, the lactobacillus plantarum can inhibit rotting for excrement well, reduce ammonia nitrogen and nitrite.The liquid
Body bacillus amyloliquefaciens can secrete antibacterial material, generate antagonism.The bacillus licheniformis can adjust flora, antagonism
Pathogenic bacteria.The bafillus natto has acidproof, heat-resistant quality beneficial bacterium, while there is the pathogen of strength to inhibit energy
Power.
The preparation method of above-mentioned deodorization complex microorganism bacterium solution is introduced below, which includes following step
It is rapid:
S1, lactobacillus plantarum, liquid starchy bacillus, bacillus licheniformis, bafillus natto are divided by bacterium number accounting
It is spare that respective fluid nutrient medium activation is not accessed；
S2, the fluid nutrient medium of complex microorganism bacterium solution is subjected to sterilization treatment and is quantified spare；
S4, the fluid nutrient medium of each strain access complex microorganism bacterium solution after activation is cultivated；
S5, the bacterium solution cultivated in the fluid nutrient medium of complex microorganism bacterium solution is detected, when bacterium solution viable count be greater than etc.
It is less than or equal to 4 in 100,000,000 CFU/ml, pH, when the color of suspension is blush, obtains deodorization complex microorganism bacterium solution of the invention.
Wherein, each strain can be obtained by existing strain, source by way of purchase.The composite microbial bacteria
The fluid nutrient medium of liquid includes following component by mass percentage: 4 ~ 6g of peptone, 12 ~ 18g of starch, 80 ~ 130g of brown sugar, agar
4 ~ 6g, 4 ~ 6g of sodium chloride, 800 ~ 1200ml of water.Preferably, the fluid nutrient medium of the complex microorganism bacterium solution presses quality percentage
Than including following component: peptone 5g, starch 15g, brown sugar 100g, agar 5g, sodium chloride 5g, water 1000ml.It is described compound micro-
The pH of the fluid nutrient medium of biological bacterium solution is 6 ~ 7.
Below with reference to a preferred embodiment, illustrate to the preparation method of deodorization complex microorganism bacterium solution of the invention
Illustrate:
It is 25% by lactobacillus plantarum, liquid starchy bacillus is 25%, bacillus licheniformis 25%, bafillus natto
It is spare that respective fluid nutrient medium activation is respectively connected to for 25%.There is provided formula is peptone 5g, starch 15g, brown sugar 100g, agar
The fluid nutrient medium of the complex microorganism bacterium solution of 5g, sodium chloride 5g, water 1000ml, pH less than or equal to 4, carries out sterilization treatment to it
And it is quantitative spare.Each strain access after activation is not linked into the fluid nutrient medium of complex microorganism bacterium solution, 30 DEG C of gas explosion,
Culture 72 hours.Complex microorganism bacterium solution 1L and water 50L are stirred evenly, are then sealed at 10 DEG C ~ 15 DEG C.Work as bacterium solution
Viable count be more than or equal to 100,000,000 CFU/ml, pH is less than or equal to 4, when the color of suspension is blush, it is multiple to obtain deodorization of the invention
Close microbial inoculum.
Deodorization complex microorganism bacterium solution in application, is sprayed solarization by deodorization complex microorganism bacterium solution of the invention in proportion and repeatedly
In urinal or septic tank, while ensuring that there is suitable time interval, the deodorization composite microbial sprayed between adjacent time
The use allotment ratio of object bacterium solution are as follows: bacterium: water=2:100.
In a preferred embodiment, 10L deodorization complex microorganism is poured into for the first time into the water closet septic tank of 20m3
10L deodorization complex microorganism bacterium solution was poured into after 4 days in bacterium solution, interval for the second time, then was spaced after 8 days that pour into 10L deodorization for the third time multiple
Close microbial inoculum.Ammonia concentration and concentration of hydrogen sulfide are measured after 5 days, while the control of bacterium solution, measurement result is not added
It is as follows:
| Testing index | Control | Bacterium solution | Reduced rate (%) |
| Ammonia concentration (mg/m3) | 5.4 | 2.03 | 62.4% |
| Concentration of hydrogen sulfide (mg/m3) | 0.021 | Nothing | 100% |
As seen from the above table, after pouring into composite bacteria liquid in water closet septic tank, ammonia and concentration of hydrogen sulfide are substantially reduced than control,
Middle ammonia concentration reduces by 62.4%, and concentration of hydrogen sulfide is reduced to nothing.In addition, this deodorization complex microorganism bacterium solution can also inhibit big
The growth of enterobacteria, Salmonella, vibrio marinopraesens, Mycophyta, the harmful worm's ovum of bacterium class and part.Lavatory excrement can also be extended
Pond uses the time, so as to accomplish the effect of deodorization is maintained.
In conclusion deodorization complex microorganism bacterium solution of the invention can quite be grown in septic tank, advantageous ecology is occupied
Position, it is suppressed that generate stench microbial reproduction, reduce stench release strength, realize the control of stench original position source.Of the invention
Deodorization complex microorganism bacterium solution containing a variety of catabolic enzymes, protein decomposition enzyme, amylolytic enzyme, lipolytic enzyme, cellulolytic enzyme,
Xylan catabolic enzyme can effectively decompose a variety of organic dirts and pernicious gas.Deodorization complex microorganism bacterium solution of the invention is realized
The efficient combination of strain, the bacterium solution after combination are significantly improved than single bacterium deodorizing effect.Deodorization composite microbial bacteria of the invention
Liquid preservation convenience, long service life.Deodorization complex microorganism bacterium solution of the invention is pollution-free, will not be flat to ambient enviroment and ecology
Weighing apparatus causes damages.Deodorization complex microorganism bacterium solution use aspect of the invention, packaging, transport convenience, easy commercialization.
It is obvious to a person skilled in the art that invention is not limited to the details of the above exemplary embodiments, Er Qie
In the case where without departing substantially from spirit or essential attributes of the invention, the present invention can be realized in other specific forms.Therefore, no matter
From the point of view of which point, the present embodiments are to be considered as illustrative and not restrictive, and the scope of the present invention is by appended power
Benefit requires rather than above description limits, it is intended that all by what is fallen within the meaning and scope of the equivalent elements of the claims
Variation is included within the present invention.
In addition, it should be understood that although this specification is described in terms of embodiments, but not each embodiment is only wrapped
Containing an independent technical solution, this description of the specification is merely for the sake of clarity, and those skilled in the art should
It considers the specification as a whole, the technical solutions in the various embodiments may also be suitably combined, forms those skilled in the art
The other embodiments being understood that.
Claims (7)
1. a kind of deodorization complex microorganism bacterium solution, which is characterized in that the bacterium number of each strain in the deodorization complex microorganism bacterium solution
Accounting are as follows:
Lactobacillus plantarum is 20 ~ 30%, and liquid starchy bacillus is 20 ~ 30%, and bacillus licheniformis is 20 ~ 30%, natto bud
Spore bacillus is 20 ~ 30%.
2. deodorization complex microorganism bacterium solution according to claim 1, which is characterized in that the deodorization complex microorganism bacterium solution
In each strain bacterium number accounting are as follows:
Lactobacillus plantarum is 25%, and liquid starchy bacillus is 25%, bacillus licheniformis 25%, and bafillus natto is
25%。
3. a kind of preparation method of deodorization complex microorganism bacterium solution as claimed in claim 1 or 2, which is characterized in that the system
Preparation Method includes the following steps:
S1, lactobacillus plantarum, liquid starchy bacillus, bacillus licheniformis, bafillus natto are divided by bacterium number accounting
It is spare that respective fluid nutrient medium activation is not accessed；
S2, the fluid nutrient medium of complex microorganism bacterium solution is subjected to sterilization treatment and is quantified spare；
S4, the fluid nutrient medium of each strain access complex microorganism bacterium solution after activation is cultivated；
S5, the bacterium solution cultivated in the fluid nutrient medium of complex microorganism bacterium solution is detected, when bacterium solution viable count be greater than etc.
It is less than or equal to 4 in 100,000,000 CFU/ml, pH, when the color of suspension is blush, obtains deodorization complex microorganism bacterium solution of the invention.
4. the preparation method of deodorization complex microorganism bacterium solution according to claim 3, which is characterized in that the composite microbial
The fluid nutrient medium of object bacterium solution includes following component by mass percentage:
4 ~ 6g of peptone, 12 ~ 18g of starch, 80 ~ 130g of brown sugar, 4 ~ 6g of agar, 4 ~ 6g of sodium chloride, 800 ~ 1200ml of water.
5. the preparation method of deodorization complex microorganism bacterium solution according to claim 4, which is characterized in that the composite microbial
The fluid nutrient medium of object bacterium solution includes following component by mass percentage: peptone 5g, starch 15g, brown sugar 100g, agar 5g,
Sodium chloride 5g, water 1000ml.
6. the preparation method of deodorization complex microorganism bacterium solution according to claim 4, which is characterized in that the composite microbial
The pH of the fluid nutrient medium of object bacterium solution is 6 ~ 7.
7. a kind of application method of deodorization complex microorganism bacterium solution as claimed in claim 1 or 2, which is characterized in that described to answer
Included the following steps: with method
Deodorization complex microorganism bacterium solution is sprayed into solarization in urinal or septic tank in proportion and repeatedly, while being ensured between adjacent time
With suitable time interval, the use allotment ratio for the deodorization complex microorganism bacterium solution sprayed are as follows: bacterium: water=2:100.
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Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
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| CN103540539A (en) * | 2013-09-27 | 2014-01-29 | 中国科学院成都生物研究所 | Microbial deodorizing microbial inoculum as well as preparation method and application thereof |
| CN103649303A (en) * | 2011-01-12 | 2014-03-19 | 伊诺库克技术股份有限公司 | Microbial compositions and methods |
| CN105420147A (en) * | 2015-12-01 | 2016-03-23 | 胡艳晖 | Compound microbial preparation for treating black and odorous rivers through strengthened calcium nitrate |
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-
2018
- 2018-12-03 CN CN201811464186.0A patent/CN109370952A/en active Pending
Patent Citations (5)
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| CN101712937A (en) * | 2008-10-06 | 2010-05-26 | 重庆尚美环保技术有限公司 | Microbial agent used for cleaning freeness and deodorization of septic tank and preparation method thereof |
| CN103649303A (en) * | 2011-01-12 | 2014-03-19 | 伊诺库克技术股份有限公司 | Microbial compositions and methods |
| CN103540539A (en) * | 2013-09-27 | 2014-01-29 | 中国科学院成都生物研究所 | Microbial deodorizing microbial inoculum as well as preparation method and application thereof |
| CN105420147A (en) * | 2015-12-01 | 2016-03-23 | 胡艳晖 | Compound microbial preparation for treating black and odorous rivers through strengthened calcium nitrate |
| CN107836567A (en) * | 2017-11-22 | 2018-03-27 | 山东信得科技股份有限公司 | A kind of deoderizing feed addictive, preparation method, application method and evaluation method |
Non-Patent Citations (2)
| Title |
|---|
| XING YAN等: "Enhanced deodorization and sludge reduction in situ by a humus soil cooperated anaerobic/anoxic/oxic (A2O) wastewater treatment system", 《ENVIRONMENTAL SCIENCE AND POLLUTION RESEARCH》 * |
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Application publication date: 20190222 |