CN108531412A - A kind of preparation method of ECMD Efficient Rings control microbial deodorant - Google Patents

A kind of preparation method of ECMD Efficient Rings control microbial deodorant Download PDF

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CN108531412A
CN108531412A CN201810228869.XA CN201810228869A CN108531412A CN 108531412 A CN108531412 A CN 108531412A CN 201810228869 A CN201810228869 A CN 201810228869A CN 108531412 A CN108531412 A CN 108531412A
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peptone
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高明
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Dalian Three Net Group Co Ltd
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Abstract

The preparation method of ECMD Efficient Ring control microbial deodorants is related to a kind of preparation method of microbial deodorant.Mainly it is to solve the problems, such as existing biological deodorant deodorizing effect difference and invent.Strain forms and volume ratio is：S. cervisiae 20 30%, bacillus subtilis 20 30%, bacillus amyloliquefaciens 10 20%, lactobacillus acidophilus 10 20%, enterococcus faecalis 10 20%, streptomyces microflavus 20 30%；S. cervisiae one ring of inoculation activates spare in YPD solid mediums；Bacillus subtilis and bacillus amyloliquefaciens one ring of inoculation activate spare in beef extract-peptone solid medium；Lactobacillus acidophilus and enterococcus faecalis be inoculated with a ring activated in MRS solid mediums it is spare, streptomyces microflavus be inoculated with a ring in Gao Shi synthesize activated in No.1 solid medium it is spare；The strain of activation is respectively connected to shaken cultivation in fluid nutrient medium；Each strain is mixed.Advantage is good deodorization effect.

Description

A kind of preparation method of ECMD Efficient Rings control microbial deodorant
Technical field：
The present invention relates to a kind of preparation methods of microbial deodorant.Specifically assisted using the symbiosis alternate between multiple bacterial strains The interaction relationships such as same-action, the microbial deodorant being built into.
Background technology：
With the adjustment of China's structure of agriculture and village economy, storage herds aquaculture and obtains continuing health quickly development, is improving Living standards of the people, while ensure that town and country animal products stablizes supply, every year up to 3,000,000,000 tons of feces of livestock and poultry causes The problem of foul gas pollution environment of getting worse.In foul gas contain a large amount of poisonous and harmful elements, mainly include ammonia, The discharge of hydrogen sulfide, mercaptan and methyl mercaptan, foul gas brings very big harm to breeding environment, influences the healthy growth of animal, Injury will also result in health after people's sucking for a long time, therefore carry out control pollution, remove the research of foul gas very It is important.In addition serious pollution is also resulted in underground water and air.
Currently, feces of livestock and poultry deodorant is broadly divided into two major classes：Chemical deodorizing agent and biological deodorant.Chemical deodorizing agent has There is the features such as quick, with strong points, but its application range is small, it is single to foul gas removal, it is multiple to encounter foul gas ingredient When miscellaneous, deodorizing effect reduces, and chemical residual will cause secondary pollution to environment.
Biological deodorizing principle is directly degraded to stench by external source function strain or biological enzyme or to generating the micro- life of stench Object inhibits to remove stench.Deodorization functions microbial bacterial agent is divided into single bacterium and more bacterium property two major classes, single bacterium at present Microbial inoculum is made of simple function bacterial strain, and more bacterium property are made of two or more function stems.Many important biochemical process single plants Microorganism cannot complete or faint can only carry out, and need under the synergistic effect of two or more microorganisms could preferably it is complete At；More bacterium property microbial inoculums have that deodorizing effect is undesirable.
Invention content：
It is main for poultry house, waste storage pool of livestock and poultry farm etc. that technical problem to be solved by the invention is to provide a kind of Stench source of release uses, and can inhibit the growth and breeding for producing stench microorganism, accelerate the decomposition of foul smell, realize stench in-situ control Efficient Ring control microbial deodorant preparation method.
What above-mentioned purpose was realized in：The strain of the deodorant forms and compatibility volume ratio is：S. cervisiae 20- 30%, bacillus subtilis 20-30%, bacillus amyloliquefaciens 10-20%, lactobacillus acidophilus 10-20%, enterococcus faecalis 10-20%, Streptomyces microflavus 20-30%；
Preferred volume ratio is：Saccharomyces cerevisiae 20%, bacillus subtilis 20%, bacillus amyloliquefaciens 15%, lactobacillus acidophilus 15%, excrement Enterococcus 10%, streptomyces microflavus 20%.
Prepare culture medium：
One, YPD solid medium are used for the activation of S. cervisiae, are formulated and are：20 grams of peptone, 10 grams of beef extract, glucose 20 grams, the pH value of 1000 milliliters of water, 18 grams of agar, YPD solid mediums is 6.5--7.0；
Two, MRS solid medium are used for the activation of lactobacillus acidophilus and enterococcus faecalis, are formulated and are：10 grams of peptone, beef extract 10 grams, 5 grams of yeast extract, 5 grams of glucose, 5 grams of sodium acetate, 2 grams of dibasic ammonium citrate, 1 milliliter of Tween-80,0.05 gram of manganese sulfate, The pH value of 0.58 gram of magnesium sulfate, 2 grams of dipotassium hydrogen phosphate, 18 grams of agar, 1000 milliliters of water, MRS solid mediums is 6.2；
Three, beef extract-peptone solid medium is used for bacillus subtilis, and bacillus amyloliquefaciens activation is formulated and is：Beef 5 grams of cream, 10 grams of peptone, 5 grams of sodium chloride, 18 grams of agar, 1000 milliliters of water, the pH value of beef extract-peptone solid medium 7.0-7.5；
Four, Gause I solid medium activates for streptomyces microflavus, is formulated and is：20 grams of starch, 1 gram of potassium nitrate, phosphoric acid hydrogen 0.5 gram of dipotassium, 0.5 gram of magnesium sulfate, 0.5 gram of sodium chloride, 0.01 gram of green vitriol, 18 grams of agar, 1000 milliliters of water are high The pH value 7.2-7.4 of family name's No.1 solid medium；
It is also useful for the fluid nutrient medium of microbial deodorant fermentation, is formulated and is：5 grams of peptone, beef extract 10,5 grams of yeast extract, 5 grams of sodium chloride, 20 grams of glucose, 0.58 gram of magnesium sulfate, 2 grams of dipotassium hydrogen phosphate, 1000 milliliters of water are sent out for microbial deodorant The pH value 6.0-7.0 of the fluid nutrient medium of ferment；
After the raw material mixing of each culture medium, spare, 121 DEG C of temperature after high-temperature sterilization processing, 30 minutes time, sterilizing are all carried out Equipment is autoclave；
Actication of culture：S. cervisiae is inoculated with a ring（One oese）It is activated in YPD solid mediums spare；Withered grass gemma Bacillus and bacillus amyloliquefaciens be inoculated with respectively a ring activated in beef extract-peptone solid medium it is spare；Lactobacillus acidophilus Be inoculated with respectively with enterococcus faecalis a ring activated in MRS solid mediums it is spare, streptomyces microflavus be inoculated with a ring in Gao Shi synthesize It is activated in No.1 solid medium spare；
The strain of activation is respectively connected to shaken cultivation in fluid nutrient medium（Shaken cultivation equipment is shaken cultivation case, oscillation training It is 30 DEG C to support temperature, and revolution is 160 revs/min）, wherein S. cervisiae, bacillus subtilis, bacillus amyloliquefaciens culture 24 hours, enterococcus faecalis culture 48 hours, streptomyces microflavus culture 96 hours, lactobacillus acidophilus stationary culture 48 hours；
The content of various bacterium is respectively：Niang brewer yeasts Jun≤8 × 108CFU/ milliliters, Jun≤3 × 10 Ku grass Ya spore Gan8CFU/ millis It rises, solves shallow lake powder bacillus≤3 × 108CFU/ milliliters, thermophilic acid lactobacillus≤5 × 108CFU/ milliliters, Jun≤4 Fen Chang Qiu × 108CFU/ milliliters, thin Jun≤5 × 10 Huang Lian Mei8CFU/ milliliters；
Each strain is mixed and obtains Efficient Ring control microbial deodorant by volume ratio as described above.Deodorant it is effective Viable count is 5 × 109CFU/ml。
Application method：
This Efficient Ring control microbial deodorant is suitable for livestock and poultry farm poultry house, treatment for cow manure pond etc., application method be The dosage of farm's poultry house, every 1000 meters of pig house henhouses or 10,000 chickens is 5 kilograms, with water 1 when use:1 blends, and makes for the first time With continuous spraying 3 days, sprays 2 times weekly later, can quickly handle stench, achieve the purpose that the depollution of environment.Treatment for cow manure pond, often 50 cubes for the treatment of for cow manure ponds use 1 liter of deodorant, and 1:After 1 is watered dilution, it is protected from light even spraying twice a week.
Advantage for present invention：
1：The present invention is made of multiple-microorganism, and the symbiosis alternate synergistic effect of these bacterial strains inhibits the life of production stench microorganism Long breeding, accelerates the decomposition of foul smell, realizes stench in-situ control.
2：Microbial inoculum of the present invention is by S. cervisiae, bacillus subtilis, bacillus amyloliquefaciens, lactobacillus acidophilus, excrement intestines The beneficial microbes such as coccus and streptomyces microflavus form, their raised growths in waste are bred, and fly mosquito ovum, coccidia ovum etc. are inhibited Harmful breeding of the ovum in excrement, is effectively improved excrement ecological environment.
3：The present invention realizes the efficient combination of multi-cultur es, and the deodorizing effect than single culture significantly improves, and experiment shows Deodorizing rate can reach 65% or more.
4：The present invention is microbial bacterial agent, and by fungi, actinomyces and bacterium three categories composition are vdiverse in function to complement each other, Efficient degradation foul gas while inhibiting production stench bacterium growth, continuous self-reproduction realize the benign cycle of deodorization, make to remove Smelly effect is more lasting, and does not have secondary pollution.
Specific implementation mode：
The strain of the deodorant forms and compatibility volume ratio is：S. cervisiae 20-30%, bacillus subtilis 20-30%, Xie Dian Afnyloliquefaciens 10-20%, lactobacillus acidophilus 10-20%, enterococcus faecalis 10-20%, streptomyces microflavus 20-30%；
Preferred volume ratio is：Saccharomyces cerevisiae 20%, bacillus subtilis 20%, bacillus amyloliquefaciens 15%, lactobacillus acidophilus 15%, excrement Enterococcus 10%, streptomyces microflavus 20%.
Prepare culture medium：
One, YPD solid medium are used for the activation of S. cervisiae, are formulated and are：20 grams of peptone, 10 grams of beef extract, glucose 20 grams, 1000 milliliters of water, 18 grams of agar, pH value 6.5--7.0；
Two, MRS solid medium are used for the activation of lactobacillus acidophilus and enterococcus faecalis, are formulated and are：10 grams of peptone, beef extract 10 grams, 5 grams of yeast extract, 5 grams of glucose, 5 grams of sodium acetate, 2 grams of dibasic ammonium citrate, 1 milliliter of Tween-80,0.05 gram of manganese sulfate, 0.58 gram of magnesium sulfate, 2 grams of dipotassium hydrogen phosphate, 18 grams of agar, 1000 milliliters of water, pH value 6.2；
Three, beef extract-peptone solid medium is used for bacillus subtilis, and bacillus amyloliquefaciens activation is formulated and is：Beef 5 grams of cream, 10 grams of peptone, 5 grams of sodium chloride, 18 grams of agar, 1000 milliliters of water, pH value 7.0-7.5；
Four, Gause I solid medium activates for streptomyces microflavus, is formulated and is：20 grams of starch, 1 gram of potassium nitrate, phosphoric acid hydrogen 0.5 gram of dipotassium, 0.5 gram of magnesium sulfate, 0.5 gram of sodium chloride, 0.01 gram of green vitriol, 18 grams of agar, 1000 milliliters of water, pH Value 7.2-7.4；
It is also useful for the fluid nutrient medium of microbial deodorant fermentation, is formulated and is：5 grams of peptone, beef extract 10,5 grams of yeast extract, 5 grams of sodium chloride, 20 grams of glucose, 0.58 gram of magnesium sulfate, 2 grams of dipotassium hydrogen phosphate, 1000 milliliters of water, pH value 6.0-7.0；
After the raw material of each culture medium is mixed, spare after progress high-temperature sterilization processing, 121 DEG C of temperature, 30 minutes time, sterilizing is set Standby is autoclave；
Actication of culture：S. cervisiae is inoculated with a ring and activates spare, bacillus subtilis reconciliation shallow lake in YPD solid mediums Afnyloliquefaciens are inoculated with a ring ring and activate spare, lactobacillus acidophilus and excrement intestines ball in beef extract-peptone solid medium respectively Bacterium be inoculated with respectively a ring activated in MRS solid mediums it is spare, streptomyces microflavus be inoculated with a ring in Gao Shi synthesize No.1 solid It is activated in culture medium spare；
The strain of activation is respectively connected to shaken cultivation in fluid nutrient medium（Shaken cultivation equipment is shaken cultivation case, oscillation training It is 30 DEG C to support temperature, and revolution is 160 revs/min）, wherein S. cervisiae, bacillus subtilis, bacillus amyloliquefaciens culture 24 hours, enterococcus faecalis culture 48 hours, streptomyces microflavus culture 96 hours, lactobacillus acidophilus stationary culture 48 hours；
The content of various bacterium is respectively：Niang brewer yeasts Jun≤8 × 108CFU/ milliliters, Jun≤3 × 10 Ku grass Ya spore Gan8CFU/ millis It rises, solves shallow lake powder bacillus≤3 × 108CFU/ milliliters, thermophilic acid lactobacillus≤5 × 108CFU/ milliliters, Jun≤4 Fen Chang Qiu × 108CFU/ milliliters, thin Jun≤5 × 10 Huang Lian Mei8CFU/ milliliters；
Each strain is mixed and obtains Efficient Ring control microbial deodorant by volume ratio as described above.Deodorant it is effective Viable count is 5 × 109CFU/ml。
Embodiment 1,
The volume ratio of six kinds of strains is：S. cervisiae 25%, bacillus subtilis 20%, bacillus amyloliquefaciens 10%, acidophilus breast Bacillus 15%, enterococcus faecalis 10%, streptomyces microflavus 20%；
S. cervisiae is accessed into an oese and activates spare, bacillus subtilis reconciliation starch bud in YPD solid mediums Spore bacillus is respectively connected to an oese and activates spare, lactobacillus acidophilus and enterococcus faecalis in beef extract-peptone solid medium Be respectively connected to an oese activated in MRS solid mediums it is spare, streptomyces microflavus access an oese in Gao Shi synthesize one It is activated in number solid medium spare；
The strain of activation is respectively connected to shaken cultivation in fluid nutrient medium, wherein saccharomyces cerevisiae, bacillus subtilis, solution starch Bacillus is cultivated 24 hours, enterococcus faecalis culture 48 hours, streptomyces microflavus culture 96 hours, lactobacillus acidophilus stationary culture 48 hours；
Bacterium solution is mixed and obtains Efficient Ring control microbial deodorant, khaki, suspension has alcohol fragrance.
The No.1 henhouse of selection 10,000 chickens of raising is 17.8mg/m using ammonia level is measured before deodorant3, hydrogen sulfide Content is 20.8mg/m3, deodorant is pressed 1 for 5 kilograms:1 is watered, and sprays henhouse, sprays within first continuous three days, and later weekly two Secondary, the tenth day measurement henhouse ammonia level is 5.9mg/m3, the content of hydrogen sulfide is 5.2 mg/m3, ammonia degradation rate is 75%, Hydrogen sulfide degradation rate is 66.8%.
Embodiment 2,
The volume ratio of six kinds of strains is：S. cervisiae 20%, bacillus subtilis 20%, bacillus amyloliquefaciens 10%, acidophilus breast Bacillus 20%, enterococcus faecalis 10%, streptomyces microflavus 20%；Preparation method is same as Example 1.
Bacterium solution is mixed and obtains Efficient Ring control microbial deodorant, khaki, suspension has alcohol fragrance.
The No.1 pig house for selecting 1000 ㎡ is 16.9mg/m using ammonia level is measured before deodorant3, hydrogen sulfide content is 12.6mg/m3, deodorant is pressed 1 for 5 kilograms:1 is watered, and sprays pig house, sprays within first continuous three days, twice a week later, the tenth Its measurement pig house ammonia level is 4.7mg/m3, hydrogen sulfide content 3.8mg/m3The degradation rate of ammonia is 72.2%, hydrogen sulfide Degradation rate is 69.8%.
Embodiment 3,
The volume ratio of six kinds of strains is：S. cervisiae 30%, bacillus subtilis 20%, bacillus amyloliquefaciens 10%, acidophilus breast Bacillus 10%, enterococcus faecalis 10%, streptomyces microflavus 20%；Preparation method is same as Example 1.
Bacterium solution is mixed and obtains Efficient Ring control microbial deodorant, khaki, suspension has alcohol fragrance.
No. two henhouses of selection 10,000 chickens of raising are 18.2mg/m using ammonia level is measured before deodorant3, hydrogen sulfide Content is 22.4mg/m3, deodorant is pressed 1 for 5 kilograms:1 is watered, and sprays henhouse, sprays within first continuous three days, and later weekly two Secondary, the tenth day measurement henhouse ammonia level is 6.3mg/m3, the content of hydrogen sulfide is 5.5 mg/m3, ammonia degradation rate is 65.4%, hydrogen sulfide degradation rate is 75.4%.
Embodiment 4,
The volume ratio of six kinds of strains is：S. cervisiae 20%, bacillus subtilis 20%, bacillus amyloliquefaciens 15%, acidophilus breast Bacillus 15%, enterococcus faecalis 10%, streptomyces microflavus 20%；Preparation method is same as Example 1.
Bacterium solution is mixed and obtains Efficient Ring control microbial deodorant, khaki, suspension has alcohol fragrance.
No. two pig houses for selecting 1000 ㎡ are 17.4mg/m using ammonia level is measured before deodorant3, hydrogen sulfide content is 13.5mg/m3, deodorant is pressed 1 for 5 kilograms:1 is watered, and sprays pig house, sprays within first continuous three days, twice a week later, the tenth Its measurement pig house ammonia level is 4.4mg/m3, hydrogen sulfide content 3.2mg/m3, the degradation rate of ammonia is 74.7%, hydrogen sulfide Degradation rate be 76.3%.
Embodiment 5,
The volume ratio of six kinds of strains is：S. cervisiae 20%, bacillus subtilis 30%, bacillus amyloliquefaciens 10%, acidophilus breast Bacillus 10%, enterococcus faecalis 10%, streptomyces microflavus 20%；Preparation method is same as Example 1.
Bacterium solution is mixed and obtains Efficient Ring control microbial deodorant, khaki, suspension has alcohol fragrance.
No. three pig houses for selecting 1000 ㎡ are 15.4mg/m using ammonia level is measured before deodorant3, hydrogen sulfide content is 12.1mg/m3, deodorant is pressed 1 for 5 kilograms:1 is watered, and sprays pig house, sprays within first continuous three days, twice a week later, the tenth Its measurement pig house ammonia level is 4.9mg/m3, hydrogen sulfide content 3.7mg/m3, the degradation rate of ammonia is 68.2%, hydrogen sulfide Degradation rate be 69.4%.
Embodiment 6,
The volume ratio of six kinds of strains is：S. cervisiae 20%, bacillus subtilis 20%, bacillus amyloliquefaciens 10%, acidophilus breast Bacillus 10%, enterococcus faecalis 10%, streptomyces microflavus 30%；Preparation method is same as Example 1.
Bacterium solution is mixed and obtains Efficient Ring control microbial deodorant, khaki, suspension has alcohol fragrance.
No. three henhouses of selection 10,000 chickens of raising are 17.9mg/m using ammonia level is measured before deodorant3, hydrogen sulfide Content is 20.7mg/m3, deodorant is pressed 1 for 5 kilograms:1 is watered, and sprays henhouse, sprays within first continuous three days, and later weekly two Secondary, the tenth day measurement henhouse ammonia level is 5.8mg/m3, the content of hydrogen sulfide is 5.1mg/m3, ammonia degradation rate is 67.6%, hydrogen sulfide degradation rate is 75.4%.

Claims (2)

1. a kind of preparation method of ECMD Efficient Rings control microbial deodorant, it is characterized in that：Strain forms and compatibility volume ratio is： S. cervisiae 20-30%, bacillus subtilis 20-30%, bacillus amyloliquefaciens 10-20%, lactobacillus acidophilus 10-20%, excrement Enterococcus 10-20%, streptomyces microflavus 20-30%；
Prepare culture medium：
One, YPD solid medium are used for the activation of S. cervisiae, are formulated and are：20 grams of peptone, 10 grams of beef extract, glucose 20 grams, 1000 milliliters of water, 18 grams of agar, pH value 6.5--7.0；
Two, MRS solid medium are used for the activation of lactobacillus acidophilus and enterococcus faecalis, are formulated and are：10 grams of peptone, beef extract 10 grams, 5 grams of yeast extract, 5 grams of glucose, 5 grams of sodium acetate, 2 grams of dibasic ammonium citrate, 1 milliliter of Tween-80,0.05 gram of manganese sulfate, 0.58 gram of magnesium sulfate, 2 grams of dipotassium hydrogen phosphate, 18 grams of agar, 1000 milliliters of water, pH value 6.2；
Three, beef extract-peptone solid medium is used for bacillus subtilis, and bacillus amyloliquefaciens activation is formulated and is：Beef 5 grams of cream, 10 grams of peptone, 5 grams of sodium chloride, 18 grams of agar, 1000 milliliters of water, pH value 7.0-7.5；
Four, Gause I solid medium activates for streptomyces microflavus, is formulated and is：20 grams of starch, 1 gram of potassium nitrate, phosphoric acid hydrogen 0.5 gram of dipotassium, 0.5 gram of magnesium sulfate, 0.5 gram of sodium chloride, 0.01 gram of green vitriol, 18 grams of agar, 1000 milliliters of water, pH Value 7.2-7.4；
It is also useful for the fluid nutrient medium of microbial deodorant fermentation, is formulated and is：5 grams of peptone, beef extract 10,5 grams of yeast extract, 5 grams of sodium chloride, 20 grams of glucose, 0.58 gram of magnesium sulfate, 2 grams of dipotassium hydrogen phosphate, 1000 milliliters of water, pH value 6.0-7.0；
After the raw material mixing of each culture medium, spare, 121 DEG C of temperature after high-temperature sterilization processing, 30 minutes time, sterilizing are all carried out Equipment is autoclave；
Actication of culture：S. cervisiae one ring of inoculation is activated in YPD solid mediums spare；Bacillus subtilis reconciliation is formed sediment Afnyloliquefaciens be inoculated with respectively a ring activated in beef extract-peptone solid medium it is spare；Lactobacillus acidophilus and enterococcus faecalis Be inoculated with respectively a ring activated in MRS solid mediums it is spare, streptomyces microflavus be inoculated with a ring in Gao Shi synthesis No.1 solid training It is activated in foster base spare；
The strain of activation is respectively connected to shaken cultivation in fluid nutrient medium, wherein S. cervisiae, bacillus subtilis, Xie Dian Afnyloliquefaciens culture 24 hours, enterococcus faecalis culture 48 hours, streptomyces microflavus culture 96 hours, lactobacillus acidophilus stand training It supports 48 hours；
Each strain is mixed and obtains Efficient Ring control microbial deodorant by volume ratio as described above.
2. the preparation method of ECMD Efficient Rings control microbial deodorant described in accordance with the claim 1, it is characterized in that：Strain compatibility Volume ratio is：Saccharomyces cerevisiae 20%, bacillus subtilis 20%, bacillus amyloliquefaciens 15%, lactobacillus acidophilus 15%, enterococcus faecalis 10%, streptomyces microflavus 20%.
CN201810228869.XA 2018-03-20 2018-03-20 A kind of preparation method of ECMD Efficient Rings control microbial deodorant Pending CN108531412A (en)

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Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN110343649A (en) * 2019-08-23 2019-10-18 浙江中益生物环保科技有限公司 A kind of microbial deoderizer and its application
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