CN109362895A - A kind of baby formula milk powder and preparation method thereof containing lactoferrin - Google Patents
A kind of baby formula milk powder and preparation method thereof containing lactoferrin Download PDFInfo
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- CN109362895A CN109362895A CN201811056272.8A CN201811056272A CN109362895A CN 109362895 A CN109362895 A CN 109362895A CN 201811056272 A CN201811056272 A CN 201811056272A CN 109362895 A CN109362895 A CN 109362895A
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23C—DAIRY PRODUCTS, e.g. MILK, BUTTER OR CHEESE; MILK OR CHEESE SUBSTITUTES; MAKING THEREOF
- A23C21/00—Whey; Whey preparations
- A23C21/06—Mixtures of whey with milk products or milk components
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23C—DAIRY PRODUCTS, e.g. MILK, BUTTER OR CHEESE; MILK OR CHEESE SUBSTITUTES; MAKING THEREOF
- A23C21/00—Whey; Whey preparations
- A23C21/04—Whey; Whey preparations containing non-milk components as source of fats or proteins
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23C—DAIRY PRODUCTS, e.g. MILK, BUTTER OR CHEESE; MILK OR CHEESE SUBSTITUTES; MAKING THEREOF
- A23C21/00—Whey; Whey preparations
- A23C21/08—Whey; Whey preparations containing other organic additives, e.g. vegetable or animal products
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23C—DAIRY PRODUCTS, e.g. MILK, BUTTER OR CHEESE; MILK OR CHEESE SUBSTITUTES; MAKING THEREOF
- A23C21/00—Whey; Whey preparations
- A23C21/10—Whey; Whey preparations containing inorganic additives
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Abstract
The invention discloses a kind of baby formula milk powder and preparation method thereof containing lactoferrin.The baby formula milk powder is by being greatly reduced the additive amount of lactoferrin, and with other each raw material rational proportions, while reducing lactoferrin additive amount, but it can pass through the reasonable cooperation of lactoferrin and other each raw materials, give full play to the bioactivity of lactoferrin, to effectively increase the efficacy of immunity of baby formula milk powder, the cost of manufacture being greatly reduced.Simultaneously by specific preparation method and corresponding raw material addition sequence, facilitate the bioactivity for playing wherein each raw material, especially lactoferrin.
Description
Technical field
The invention belongs to milk powder preparation technical fields, and in particular to a kind of baby formula milk powder containing lactoferrin and its
Preparation method.
Background technique
Lactoferrin is a kind of glycosylated protein that can combine iron ion, belongs to a member in transferrins family, mainly
It is distributed in the milk of mammal, the content highest in people's colostrum, about 5~7g/L, about 1~2g/L in mature cream
(Hennart PF, 1991), in cow's milk lactoferrin content than much less in human milk, only about 0.1g/L (Sanchez L,
1988).A large number of studies show that lactoferrin has antibacterial and antivirus action, this may be since lactoferrin and iron ion have
There is high-affinity, iron can be competed with bacterium and play bacteriostasis, the lipopolysaccharides (LPS) with bacterium surface can also be passed through
Reciprocation destroys the outer cell membrane of bacterium, and prevents the attachment of pathogenic entero becteria Escherichia coli and prevent the invasion of virus
(Valenti P,et al.2005；Susana A.GC,2009).
Thus, lactoferrin is applied in milk powder, such as Chinese patent literature CN105746713A discloses one
Kind promotes the babies ' formula milk powder of newborn's Intestinal Mucosal Immunity functional development.The newborn iron egg of 3%-5% is added in the technology
It is white, and in addition it is added to the lysozyme of 0.2%-0.3%.Enrich to a certain extent the nutrition of babies ' formula milk powder at
Point.
In above-mentioned technology, lactoferrin content is higher, is only added into as a kind of enriched nutritive component for promoting product
Class, but lactoferrin is more sensitive to heat, and thermal stability is poor, requires environment pH also more stringent, excessively cannot
The lactoferrin of effective biological action is played, not only causes product price to increase, the wasting of resources, while can also affect on baby
Effective performance of other components in youngster's formula milk finally influences the abundant of the efficacy of immunity of the babies ' formula milk powder containing it
It plays.
For this purpose, how to provide a kind of good baby formula milk powder containing lactoferrin of at low cost, efficacy of immunity and its
Preparation method is this field technical problem urgently to be resolved.
Summary of the invention
For this purpose, to be solved by this invention, to be existing babies ' formula milk powder lacked there are at high cost, efficacy of immunity is not high
Fall into, and then a kind of at low cost, the high baby formula milk powder containing lactoferrin of efficacy of immunity is provided and preparation method thereof.
In order to solve the above technical problems, The technical solution adopted by the invention is as follows:
Baby formula milk powder provided by the present invention containing lactoferrin, including following raw material: desalted whey powder
25-50 parts, 15-30 parts of vegetable oil, 10-40 parts of skimmed milk powder, 1-9 parts of lactose, 2-12 parts of galactooligosaccharide, two dodecahexaenes
0.1-1 parts sour, 0.2-2 parts of arachidonic acid, 0.02-0.2 parts of choline chloride, 0.01-0.1 parts of inositol, L-carnitine 0.01-
0.1 part, 0.01-0.1 parts of nucleotide, 0.01-0.1 parts of casein phosphopeptide, 0.1-1 parts of lactoferrin, minerals 0.05-0.5
Part, 0.03-0.3 parts of vitamin, 0.01-0.3 parts of nutrient.
Further, the vegetable oil is mixed vegetable oil, and the mixed vegetable oil includes palm oil, sunflower oil, dish
Seed oil, palm-kernel oil, maize oil plus coconut oil, the palm oil, sunflower oil, rapeseed oil, palm-kernel oil, corn oil and coconut
The mass ratio of oil is (25-45): (15-40): (15-40): (2-6): (1-6): (1-5).
Further, the vitamin includes Vitamin D3, dl- α-D-α-tocopherol acetate, phytondione, thiamine hydrochloride
Element, riboflavin, puridoxine hydrochloride, cyanocobalamin, niacinamide, D-Biotin, inositol, folic acid, D-VB5 calcium and taurine；
The Vitamin D3, dl- α-D-α-tocopherol acetate, phytondione, thiamine hydrochloride, riboflavin, puridoxine hydrochloride,
Cyanocobalamin, niacinamide, D-Biotin, inositol, folic acid, D-VB5 calcium and taurine mass ratio be (1-3): (5-25): (0.4-
1.2): (0.1-1): (0.02-0.2): (0.1-0.5): (0.5-1.5): (1-5): (0.003-0.3): (10-25): (0.01-
0.2): (0.3-3): (10-30).
Further, the minerals include ferrous sulfate, zinc sulfate, sodium selenite, magnesium sulfate, copper sulphate, manganese sulfate
And citric acid, the ferrous sulfate, zinc sulfate, sodium selenite, magnesium sulfate, copper sulphate, manganese sulfate and citric acid mass ratio be
(4-40): (2-10): (0.5-3.5): (40-60): (0.1-1): (0.02-2): (0.1-1.0).
Further, the nucleotide includes 5'-CMP, 5'-monophosphate adenosine, 5 '-creatinine acid disodiums, 5 '-
Sodium guanylate and 5'-UMP disodium；
The 5'-CMP, 5'-monophosphate adenosine, 5 '-creatinine acid disodiums, 5'-GMP2Na and 5 '-uridines
The mass ratio of acid disodium is (10-25): (5-15): (5-15): (2-10): (10-25).
Further, the nutrient includes retinol acetate, L-AA sodium and lutein, and the acetic acid view is yellow
The mass ratio of ester, L-AA sodium and lutein is (1-5): (35-70): (5-20).
It further, further include oligofructose, the parts by weight of the oligofructose are not more than 1 part.
In addition, including the following steps: the present invention also provides the preparation method of above-mentioned baby formula milk powder
By desalted whey powder, skimmed milk powder, lactose, galactooligosaccharide, choline chloride, inositol, L-carnitine and nucleotide
It is soluble in water, obtain the first mixture；
Vegetable oil is added in first mixture, and carries out homogeneous, obtains the second mixture；
Vitamin and mineral is added into second mixture, and is successively mixed, sterilized, be concentrated and dried,
Obtain base-material；
Lactoferrin, casein phosphopeptide, docosahexaenoic acid, arachidonic acid and nutrition are added into the base-material
Element, progress is dry-mixed, obtains the baby formula milk powder.
Further, the temperature of the water is 40-65 DEG C, and the temperature of the vegetable oil is 40-65 DEG C；
The pressure of the homogeneous is 100-200bar, pressure is 40-65 DEG C；
The temperature of the sterilization is 75-95 DEG C, time 20-90s；
The temperature of the concentration is 70-90 DEG C.
Further, the drying successively includes spray drying and fluidized bed drying, the inlet air temperature of the spray drying
180 DEG C -215 DEG C, 70-90 DEG C of temperature of outgoing air, the fluidized bed drying successively includes the Ith dryer section, the IIth dryer section, the IIIth dry
Dry section and Section IV dryer section, the temperature of the Ith dryer section, the IIth dryer section and the IIIth dryer section is 15-95 DEG C, and described the
The temperature of IV dryer section is 15-40 DEG C；
The dry-mixed revolving speed is 10-30r/min, do time >=2min in advance, mixing time 5-10min.
Compared with prior art, the invention has the following beneficial effects:
(1) baby formula milk powder provided by the present invention containing lactoferrin, by the way that adding for lactoferrin is greatly reduced
Dosage, and with other each raw material rational proportions, while reducing lactoferrin additive amount, but can by lactoferrin with it is other
The reasonable cooperation of each raw material, gives full play to the bioactivity of lactoferrin, to effectively increase exempting from for baby formula milk powder
Epidemic disease power effect, while the cost of manufacture being greatly reduced.
(2) baby formula milk powder provided by the present invention containing lactoferrin, preferably vegetable oil, vitamin, minerals,
The proportion of corresponding ingredient in nucleotide and nutrient, while abundant baby formula milk powder nutritional ingredient, moreover it is possible to facilitate
The bioactivity of lactoferrin is given full play to, to effectively increase the efficacy of immunity of baby formula milk powder.
(3) preparation method of the baby formula milk powder provided by the present invention containing lactoferrin by desalted whey powder, takes off
Fat milk powder, lactose, galactooligosaccharide, choline chloride, inositol, L-carnitine and nucleotide are soluble in water, obtain the first mixture；
Vegetable oil is added in first mixture, and carries out homogeneous, obtains the second mixture；Add into second mixture
Enter vitamin and mineral, and successively mixed, sterilized, be concentrated and dried, obtains base-material；Newborn iron is added into the base-material
Albumen, casein phosphopeptide, docosahexaenoic acid, arachidonic acid and nutrient, progress is dry-mixed, obtains infant formula milk
Powder.The specific preparation method and corresponding raw material addition sequence facilitate the bioactivity for playing wherein each raw material, especially
Lactoferrin.
Detailed description of the invention
It, below will be to specific in order to illustrate more clearly of the specific embodiment of the invention or technical solution in the prior art
Embodiment or attached drawing needed to be used in the description of the prior art be briefly described, it should be apparent that, it is described below
Attached drawing is some embodiments of the present invention, for those of ordinary skill in the art, before not making the creative labor
It puts, is also possible to obtain other drawings based on these drawings.
Fig. 1 is the process flow chart of the preparation method of the baby formula milk powder in the embodiment of the present invention 1 containing lactoferrin.
Specific embodiment
Technical solution of the present invention is clearly and completely described below, it is clear that described embodiment is the present invention
A part of the embodiment, instead of all the embodiments.Based on the embodiments of the present invention, those of ordinary skill in the art are not having
Every other embodiment obtained under the premise of creative work is made, shall fall within the protection scope of the present invention.
Embodiment 1
Present embodiments provide a kind of baby formula milk powder and preparation method thereof containing lactoferrin.The baby formula
Milk powder is made of the following raw material: desalted whey powder 42.3g, vegetable oil 22.4g, skimmed milk powder 15.7g, lactose 5.96g, oligomeric half
Lactose (GOS) 7.3g, oligofructose (FOS) 0.39g, docosahexaenoic acid (DHA) 0.57g, arachidonic acid (AA) 1.1g,
Choline chloride 0.064g, inositol 0.034g, L-carnitine 0.026g, nucleotide 0.045g, casein phosphopeptide 0.035g, newborn iron
Albumen 0.5g, minerals 0.324g, vitamin 0.196g, nutrient 0.2g；
Wherein, vegetable oil be mass ratio be 35:27.5:27:4:3.5:3 palm oil, sunflower oil, rapeseed oil, palm
Benevolence oil, maize oil plus coconut oil；
Vitamin is that mass ratio is 2.2:10.4:0.82:0.56:0.11:0.32:0.95:2.9:0.01:17:0.08:
The Vitamin D3 of 1.97:23.5, dl- α-D-α-tocopherol acetate, phytondione, thiamine hydrochloride, riboflavin, puridoxine hydrochloride,
Cyanocobalamin, niacinamide, D-Biotin, inositol, folic acid, D-VB5 calcium and taurine；
Minerals be mass ratio be 5.8:4.32:0.96:56.9:0.31:0.093:0.77 ferrous sulfate, zinc sulfate,
Sodium selenite, magnesium sulfate, copper sulphate, manganese sulfate and citric acid；
Nucleotide be mass ratio be 17.56:11.56:10.44:4.67:17.56 5'-CMP, 5 '-monophosphates
Adenosine, 5 '-creatinine acid disodiums, 5'-GMP2Na and 5'-UMP disodium；
Nutrient is retinol acetate, L-AA sodium and the lutein that mass ratio is 3.65:55.55:13.7；
The preparation method of above-mentioned baby formula milk powder, as shown in Figure 1, including the following steps:
By water-soluble formulations (desalted whey powder, skimmed milk powder, lactose, galactooligosaccharide, oligofructose, choline chloride, flesh
Alcohol, L-carnitine and nucleotide) it puts into the pure water for being heated to 60 DEG C, obtain the first mixture；
In vegetable oil it is various oil by premixing after be heated to 60 DEG C, be added in the first mixture and mix, in 60 DEG C,
4 DEG C are cooled under 150bar after homogeneous, obtains the second mixture；
Vitamin and mineral is added into the second mixture, after mixing evenly, 30s is successively sterilized at 88 DEG C, in 86
Concentration at DEG C is spray-dried at 200 DEG C of inlet air temperature and 80 DEG C of temperature of outgoing air, using fluidized bed drying, the Ith dryer section,
The temperature of IIth dryer section and the IIIth dryer section is 85 DEG C, and the temperature of Section IV dryer section is 35 DEG C, obtains base-material；
After dried original washing powder vibration screening, jumbo bag packaging is carried out by powder transportation system, is stored in this, as original washing powder standby
With；
When carrying out finished product production according to scheduling of production, after original washing powder again vibration screening, entered by powder transportation system dry
Lactoferrin, casein phosphopeptide, docosahexaenoic acid, arachidonic acid and by nutrition is added in mixed tank during transportation
The mixed dry-mixed nutrient premix packet of element reaches and carries out after dry-mixed tank dry-mixed, and do time 5min in advance, mixing time 8min, mixes
Material machine revolving speed 20r/min；
Dry-mixed good milk powder is suitble to 0-6 monthly age baby by packaging, X-ray examination, qualified products vanning storage and transportation, the milk powder
Youngster.
Embodiment 2
Present embodiments provide a kind of baby formula milk powder and preparation method thereof containing lactoferrin.The baby formula
Milk powder is made of the following raw material: desalted whey powder 33.6g, vegetable oil 17.7g, skimmed milk powder 33.5g, lactose 3.07g, oligomeric half
Lactose (GOS) 7.3g, oligofructose (FOS) 0.39g, docosahexaenoic acid (DHA) 0.50g, arachidonic acid (AA) 1.0g,
Choline chloride 0.045g, inositol 0.038g, L-carnitine 0.039g, nucleotide 0.039g, casein phosphopeptide 0.035g, newborn iron
Albumen 0.5g, minerals 0.108g, vitamin 0.197g, nutrient 0.2g；
Wherein, vegetable oil be mass ratio be 35:27.5:27:4:3.5:3 palm oil, sunflower oil, rapeseed oil, palm
Benevolence oil, maize oil plus coconut oil；
Vitamin is that mass ratio is 2.2:16.4:0.71:0.51:0.15:0.25:1.27:2.69:0.02:19.14:
Vitamin D3, dl- α-D-α-tocopherol acetate, phytondione, thiamine hydrochloride, the riboflavin, hydrochloric acid pyrrole of 0.051:1.17:23.5
It trembles alcohol, cyanocobalamin, niacinamide, D-Biotin, inositol, folic acid, D-VB5 calcium and taurine；
Minerals be mass ratio be 22.2:9.44:2.4:34.2:0.93:0.19:0.56 ferrous sulfate, zinc sulfate, Asia
Sodium selenate, magnesium sulfate, copper sulphate, manganese sulfate and citric acid；
Nucleotide be mass ratio be 17.56:11.54:10.51:4.61:17.69 5'-CMP, 5 '-monophosphates
Adenosine, 5 '-creatinine acid disodiums, 5'-GMP2Na and 5'-UMP disodium；
Nutrient is retinol acetate, L-AA sodium and the lutein that mass ratio is 3.65:55.55:13.7；
The preparation method of above-mentioned baby formula milk powder, includes the following steps:
By water-soluble formulations (desalted whey powder, skimmed milk powder, lactose, galactooligosaccharide, oligofructose, choline chloride, flesh
Alcohol, L-carnitine and nucleotide) it puts into the pure water for being heated to 60 DEG C, obtain the first mixture；
In vegetable oil it is various oil by premixing after be heated to 60 DEG C, be added in the first mixture and mix, in 60 DEG C,
4 DEG C are cooled under 150bar after homogeneous, obtains the second mixture；
Vitamin and mineral is added into the second mixture, after mixing evenly, 35s is successively sterilized at 88 DEG C, in 86
Concentration at DEG C is spray-dried at 200 DEG C of inlet air temperature and 80 DEG C of temperature of outgoing air, using fluidized bed drying, the Ith dryer section,
The temperature of IIth dryer section and the IIIth dryer section is 85 DEG C, and the temperature of Section IV dryer section is 35 DEG C, obtains base-material；
Original washing powder is sent into dry-mixed tank, lactoferrin, casein phosphopeptide, two dodecahexaenes are added during transportation
Acid, arachidonic acid and the dry-mixed nutrient premix packet mixed by nutrient reach and carry out after dry-mixed tank dry-mixed, does time in advance
5min, mixing time 8min, batch mixer revolving speed 20r/min, obtain baby formula milk powder, which is suitble to 6-12 monthly age baby
Youngster.
Embodiment 3
Present embodiments provide a kind of baby formula milk powder and preparation method thereof containing lactoferrin.The baby formula
Milk powder is made of the following raw material: desalted whey powder 29.6g, vegetable oil 17.4g, skimmed milk powder 38.6g, lactose 3.2g, oligomeric half
Lactose (GOS) 7.4g, docosahexaenoic acid (DHA) 0.20g, arachidonic acid (AA) 0.4g, choline chloride 0.073g, inositol
0.038g, L-carnitine 0.039g, nucleotide 0.039g, casein phosphopeptide 0.035g, lactoferrin 0.5g, minerals
0.247g, vitamin 0.198g, nutrient 0.2g；
Wherein, vegetable oil be mass ratio be 35:27.5:27:4:3.5:3 palm oil, sunflower oil, rapeseed oil, palm
Benevolence oil, maize oil plus coconut oil；
Vitamin is that mass ratio is 2.2:15.4:0.71:0.3:0.1:0.3:1.41:2.68:0.1:19.14:0.051:
The Vitamin D3 of 1.11:22.98, dl- α-D-α-tocopherol acetate, phytondione, thiamine hydrochloride, riboflavin, puridoxine hydrochloride,
Cyanocobalamin, niacinamide, D-Biotin, inositol, folic acid, D-VB5 calcium and taurine；
Minerals be mass ratio be 10.12:4.13:1.05:54.17:0.36:0.081:0.4 ferrous sulfate, sulfuric acid
Zinc, sodium selenite, magnesium sulfate, copper sulphate, manganese sulfate and citric acid；
Nucleotide be mass ratio be 17.46:11.39:10.63:4.81:17.47 5'-CMP, 5 '-monophosphates
Adenosine, 5 '-creatinine acid disodiums, 5'-GMP2Na and 5'-UMP disodium；
Nutrient is retinol acetate, L-AA sodium and the lutein that mass ratio is 3.65:55.55:13.7；
The preparation method of above-mentioned baby formula milk powder, includes the following steps:
By water-soluble formulations (desalted whey powder, skimmed milk powder, lactose, galactooligosaccharide, choline chloride, inositol, left-handed meat
Alkali and nucleotide) it puts into the pure water for being heated to 60 DEG C, obtain the first mixture；
In vegetable oil it is various oil by premixing after be heated to 60 DEG C, be added in the first mixture and mix, in 60 DEG C,
4 DEG C are cooled under 150bar after homogeneous, obtains the second mixture；
Vitamin and mineral is added into the second mixture, after mixing evenly, 35s is successively sterilized at 88 DEG C, in 86
Concentration at DEG C is spray-dried at 200 DEG C of inlet air temperature and 80 DEG C of temperature of outgoing air, using fluidized bed drying, the Ith dryer section,
The temperature of IIth dryer section and the IIIth dryer section is 85 DEG C, and the temperature of Section IV dryer section is 35 DEG C, obtains base-material；
Original washing powder is sent into dry-mixed tank, lactoferrin, casein phosphopeptide, two dodecahexaenes are added during transportation
Acid, arachidonic acid and the dry-mixed nutrient premix packet mixed by nutrient reach and carry out after dry-mixed tank dry-mixed, does time in advance
5min, mixing time 8min, batch mixer revolving speed 20r/min, obtain baby formula milk powder, which is suitble to 12-36 monthly age baby
Youngster.
Embodiment 4
Present embodiments provide a kind of baby formula milk powder and preparation method thereof containing lactoferrin.The baby formula
Milk powder is made of the following raw material: desalted whey powder 25g, vegetable oil 30g, skimmed milk powder 10g, lactose 9g, galactooligosaccharide (GOS)
2g, oligofructose (FOS) 1g, docosahexaenoic acid (DHA) 0.1g, arachidonic acid (AA) 2g, choline chloride 0.02g, flesh
Alcohol 0.1g, L-carnitine 0.01g, nucleotide 0.1g, casein phosphopeptide 0.01g, lactoferrin 1g, minerals 0.05g, dimension life
Plain 0.3g, nutrient 0.01g；
Wherein, vegetable oil be mass ratio be 25:40:15:6:1:5 palm oil, sunflower oil, rapeseed oil, palm-kernel oil,
Maize oil plus coconut oil；
Vitamin is the gallbladder calcification that mass ratio is 1:25:0.4:1:0.02:0.5:0.5:5:0.003:25:0.01:3:10
Alcohol, dl- α-D-α-tocopherol acetate, phytondione, thiamine hydrochloride, riboflavin, puridoxine hydrochloride, cyanocobalamin, niacinamide, D- are raw
Object element, inositol, folic acid, D-VB5 calcium and taurine；
Minerals be mass ratio be 40:2:3.5:40:1:0.02:1.0 ferrous sulfate, zinc sulfate, sodium selenite, sulfuric acid
Magnesium, copper sulphate, manganese sulfate and citric acid；
Nucleotide be mass ratio be 10:15:5:10:10 5'-CMP, 5'-monophosphate adenosine, 5 '-creatinines
Disodium, 5'-GMP2Na and 5'-UMP disodium；
Nutrient is retinol acetate, L-AA sodium and the lutein that mass ratio is 1:70:5；
The preparation method of above-mentioned baby formula milk powder, includes the following steps:
By water-soluble formulations (desalted whey powder, skimmed milk powder, lactose, galactooligosaccharide, oligofructose, choline chloride, flesh
Alcohol, L-carnitine and nucleotide) it puts into the pure water for being heated to 40 DEG C, obtain the first mixture；
In vegetable oil it is various oil by premixing after be heated to 40 DEG C, be added in the first mixture and mix, in 40 DEG C,
4 DEG C are cooled under 200bar after homogeneous, obtains the second mixture；
Vitamin and mineral is added into the second mixture, after mixing evenly, 20s is successively sterilized at 95 DEG C, in 70
Concentration at DEG C is spray-dried at 180 DEG C of inlet air temperature and 90 DEG C of temperature of outgoing air, using fluidized bed drying, the Ith dryer section,
The temperature of IIth dryer section and the IIIth dryer section is 30 DEG C, and the temperature of Section IV dryer section is 40 DEG C, obtains base-material；
Original washing powder is sent into dry-mixed tank, lactoferrin, casein phosphopeptide, two dodecahexaenes are added during transportation
Acid, arachidonic acid and the dry-mixed nutrient premix packet mixed by nutrient reach and carry out after dry-mixed tank dry-mixed, does time in advance
8min, mixing time 10min, batch mixer revolving speed 10r/min, obtain baby formula milk powder.
Embodiment 5
Present embodiments provide a kind of baby formula milk powder and preparation method thereof containing lactoferrin.The baby formula
Milk powder is made of the following raw material: desalted whey powder 50g, vegetable oil 15g, skimmed milk powder 40g, lactose 1g, galactooligosaccharide (GOS)
12g, oligofructose (FOS) 0.1g, docosahexaenoic acid (DHA) 1g, arachidonic acid (AA) 0.2g, choline chloride 0.2g,
Inositol 0.01g, L-carnitine 0.1g, nucleotide 0.01g, casein phosphopeptide 0.1g, lactoferrin 0.1g, minerals 0.5g,
Vitamin 0.03g, nutrient 0.3g；
Wherein, vegetable oil be mass ratio be 45:15:40:2:6:1 palm oil, sunflower oil, rapeseed oil, palm-kernel oil,
Maize oil plus coconut oil；
Vitamin is the gallbladder calcification that mass ratio is 3:5:1.2:0.1:0.2:0.1:1.5:1:0.3:10:0.2:0.3:30
Alcohol, dl- α-D-α-tocopherol acetate, phytondione, thiamine hydrochloride, riboflavin, puridoxine hydrochloride, cyanocobalamin, niacinamide, D- are raw
Object element, inositol, folic acid, D-VB5 calcium and taurine；
Minerals be mass ratio be 4:10:0.5:60:0.1:2:0.1 ferrous sulfate, zinc sulfate, sodium selenite, sulfuric acid
Magnesium, copper sulphate, manganese sulfate and citric acid；
Nucleotide be mass ratio be 25:5:15:2:25 5'-CMP, 5'-monophosphate adenosine, 5 '-creatinines two
Sodium, 5'-GMP2Na and 5'-UMP disodium；
Nutrient is retinol acetate, L-AA sodium and the lutein that mass ratio is 5:35:20；
The preparation method of above-mentioned baby formula milk powder, includes the following steps:
By water-soluble formulations (desalted whey powder, skimmed milk powder, lactose, galactooligosaccharide, oligofructose, choline chloride, flesh
Alcohol, L-carnitine and nucleotide) it puts into the pure water for being heated to 65 DEG C, obtain the first mixture；
In vegetable oil it is various oil by premixing after be heated to 65 DEG C, be added in the first mixture and mix, in 65 DEG C,
4 DEG C are cooled under 100bar after homogeneous, obtains the second mixture；
Vitamin and mineral is added into the second mixture, after mixing evenly, 90s is successively sterilized at 75 DEG C, in 90
Concentration at DEG C is spray-dried at 215 DEG C of inlet air temperature and 70 DEG C of temperature of outgoing air, using fluidized bed drying, the Ith dryer section,
The temperature of IIth dryer section and the IIIth dryer section is 95 DEG C, and the temperature of Section IV dryer section is 15 DEG C, obtains base-material；
Original washing powder is sent into dry-mixed tank, lactoferrin, casein phosphopeptide, two dodecahexaenes are added during transportation
Acid, arachidonic acid and the dry-mixed nutrient premix packet mixed by nutrient reach and carry out after dry-mixed tank dry-mixed, does time in advance
10min, mixing time 5min, batch mixer revolving speed 30r/min, obtain baby formula milk powder.
Test example
Respectively using the baby formula milk powder containing lactoferrin of embodiment 5, embodiment 1 and embodiment 4 as test article, adopt
Test influence of the formula milk to immune functions of rats for investigating the lactoferrin containing various dose with rat, specific test process and
Interpretation of result is as follows:
1, experimental animal selection and grouping:
Selection SPF grades SD rat 120, female, weight 50-70g.Rat is randomly divided into five groups, i.e. distilled water by weight
Control group, milk powder control group (formula in embodiment 1 in Chinese patent literature CN105746713A) and three basic, normal, high
Dosage group, every group each 10.In the formula milk of high, normal, basic three dosage the content of lactoferrin be respectively 100 (embodiments 5),
500 (embodiments 1) and 1000mg/100g (embodiment 4).The rat of three dosage groups is respectively according to 100,500 and 1000mg/kg
The amount of body weight/day is fed, daily same time stomach-filling 1 time of all animals, stomach-filling amount 1.0ml/100g weight, weekly basis
The weight of animals growth pattern adjusts stomach-filling amount, continuous gavage surrounding.
2, key instrument and reagent:
Ao Haosi SPN3001F, Sai Duolisi BS2000S, BS200S-WE1 electronic balance, Japanese photoelectricity MEK-6318K is certainly
Dynamic blood analyser, flow cytometer, microplate reader, vernier caliper etc., cell phenotype detect streaming antibody, cytokines measurement examination
Agent box.
3, main test index and method:
3.1 sheep red blood cell (SRBC)s (SRBC) induced rat DTH (vola pedis thickens method):
Sensitization: 2% (v/v) SRBC peritoneal immunity of rat, every mouse inject 0.2mL (about 1 × 108 SRBC).
The generation and measurement of DTH: 4 days after immune, measuring left back foot plantar thickness, is then subcutaneously injected in measuring point
20% (v/v) SRBC, every 20 μ L of mouse (about 1 × 108 SRBC), in measuring left back vola pedis thickness, same position for 24 hours after injection
Measurement three times, is averaged.
3.2 antibody-producting cell detections (Jerne improves slide method):
SRBC: sheep taking blood from jugular vein removes fiber, is put into 4 DEG C of refrigerators and saves backup.
Prepare complement: acquisition guinea pig blood isolates serum, 1mL hematocrit SRBC is added in 5mL guinea pig serum, 4 DEG C of ice
Case places 30min, often vibrates, centrifuging and taking supernatant, packing, -70 DEG C of preservations
Immune animal: taking de- fiber sheep blood, with brine 3 times, is centrifuged (2000r/min) 10min every every time
Mouse is through being injected intraperitoneally SRBC 5 × 107~2 × 108It is a.
Splenocyte suspension preparation: taking blood after the rat anesthesia after SRBC is immunized 4~5 days and put to death, and takes out spleen, is made
Cell suspension is cleaned twice with Hanks liquid, is settled to 10ml, counts cell.
The measurement of plaque: after surface layer culture medium is dissolved by heating, putting 45 DEG C of water-baths and keep the temperature, and 2 times with equivalent pH7.2
The mixing of Hanks liquid, then small test tube is dispensed, 50 μ L10%SRBC are added into pipe by every pipe 0.5ml, and it is thin to be eventually adding 10-20 μ L spleen
Born of the same parents' suspension mixes rapidly, is poured on the slide of brush agarose thin layer, is put into CO21.5h in incubator is dilute with SA buffer
The complement released is added in glass frame groove, continues after cultivating 1.5h, counts hemolysis plaque number.
3.3MTT method measures T lymphocyte proliferation:
Splenocyte suspension preparation: it is sterile to take spleen, it is placed in and fills in appropriate sterile Hanks liquid plate, with pintle gently by spleen
It grinds, individual cells suspension is made.Through 200 mesh net filtrations, is washed 2 times with Hanks liquid, be centrifuged 10min (1000r/ every time
min).Then cell is suspended in the RPMI1640 complete culture solution of 1mL, it (should be with platform phenol orchid dyeing counting viable count
95% or more), adjustment cell concentration is 3 × 106/mL.
Lymphproliferation response: being divided to two holes to be added in 24 well culture plates every a splenocyte suspension, every hole 1mL, and one
Hole adds 50 μ L ConA liquid (5.0 μ g/mL of final concentration), and 5%CO is set as control in another hole2, 37 DEG C of CO2It is cultivated in incubator
72h.Culture terminates preceding 4h, and every hole gently sucks supernatant 0.7mL, and RPMI1640 of the 0.7mL without calf serum is added and cultivates
Liquid, while 50 hole μ L/ MTT (5mg/ml) is added, continue to cultivate 4h.After culture, supernatant is removed, it is different that 1mL acidity is added in every hole
Propyl alcohol, piping and druming mix, and are completely dissolved purple crystal.Then it is dispensed into 96 well culture plates, 2 parallel holes are made in each hole, use
Microplate reader measures OD value with 570nm wavelength.
4 experimental results and analysis
Influence of 4.1 lactoferrins to rat body weight and immune organ weight
After nursing processing in one month, rat body weight variation is as shown in table 1, as can be seen from Table 1 various dose
Lactoferrin rat body weight growth is had no and significantly affects.And middle and high dosage lactoferrin group (500 and 1000mg/kg
Bw/d) Rats Spleen relative weight increases compared with blank control group.The relative weight of thymus gland has no between each processing group
Notable difference (is shown in Table 2).This explanation adds the lactoferrin of various dose compared with the formula milk group for not adding lactoferrin
Rat body weight and immune organ weight are without obviously increasing afterwards.
Influence of 1 lactoferrin of table to rat body weight
2 lactoferrin of table to immune organs of rats weight influence (mg/g,)
Relatively heavy=internal organs weight (mg)/weight (g)
With blank control group ratio, *: P < 0.05.
Influence of 4.2 lactoferrins to delayed allergy
Table 3 shows influence of the various dose lactoferrin to delayed allergy, as shown in table 3.It can be seen that with empty
White control group is compared, and obviously writing, which occurs, in the delayed allergy of experimental group rat (DTH) degree of three dosage to be increased；With milk
Powder control group is compared, middle and high dosage lactoferrin group (500 and 1000mg/kgbw/d) rat delayed allergy (DTH)
Degree significantly increases (table 4).
Influence of 3 lactoferrin of table to rat DTH
With blank control group ratio, * *: P < 0.01；With milk powder contrast ratio, #:P < 0.05, ##:P < 0.01
Influence of 4.3 lactoferrins to the ConA rat spleen lymphocytes transformation function stimulated
Table 4 shows the Rats Spleen Function of lymphocyte transform that various dose lactoferrin stimulates ConA in formula milk
Influence.As a result, it has been found that compared with blank control group, middle and high dosage lactoferrin group (500 and 1000mg/kgbw/d) rat
The spleen lymphocyte conversion capability of ConA induction significantly increases；(table 5) also is significantly increased compared with milk powder control group simultaneously.
Influence of 4 lactoferrin of table to the ConA rat spleen lymphocytes transformation function stimulated
With blank control group ratio, *: P < 0.05, * *: P < 0.01；With milk powder contrast ratio, #:P < 0.05, ##:P < 0.01
Influence of 4.4 lactoferrins to full spleen plaque forming cells number
Table 5 shows influence of the lactoferrin of various dose to full spleen plaque forming cells number.As a result, it has been found that and blank
Control is compared with milk powder control group, the IgM-PFC number/full spleen and IgM-PFC/ of the lactoferrin rats in test groups of three dosage
106The increase of the equal conspicuousness of splenocyte.
Influence of 5 lactoferrin of table to rat IgM-PFC/ spleen
With blank control group ratio, *: P < 0.05, * *: P < 0.01；With milk powder contrast ratio, #:P < 0.05##:P < 0.01
These results suggest that lactoferrin is added with the dosage of 500-1000mg/100g in formula milk, after feeding four weeks
Rat compared with the formula milk group of no lactoferrin immune organ weight without significantly increasing.But contain middle high dose (500-
1000mg/100g) formula milk of lactoferrin shows significantly to promote to make to DTH and the T lymphocyte proliferation of rat
With showing lactoferrin, it has effects that enhance cellular immunity；High dose (500-1000mg/100g) is empty to full spleen in simultaneously
Spot forms cell number (PFC) and shows apparent humidification, it is believed that the lactoferrin of this dosage range can promote body fluid
Immune function.
Obviously, the above embodiments are merely examples for clarifying the description, and does not limit the embodiments.It is right
For those of ordinary skill in the art, can also make on the basis of the above description it is other it is various forms of variation or
It changes.There is no necessity and possibility to exhaust all the enbodiments.And it is extended from this it is obvious variation or
It changes still within the protection scope of the invention.
Claims (10)
1. a kind of baby formula milk powder containing lactoferrin, including following raw material: 25-50 parts of desalted whey powder is planted
15-30 parts of object oil, 10-40 parts of skimmed milk powder, 1-9 parts of lactose, 2-12 parts of galactooligosaccharide, 0.1-1 parts of docosahexaenoic acid,
0.2-2 parts of arachidonic acid, 0.02-0.2 parts of choline chloride, 0.01-0.1 parts of inositol, 0.01-0.1 parts of L-carnitine, nucleotide
0.01-0.1 parts, 0.01-0.1 parts of casein phosphopeptide, 0.1-1 parts of lactoferrin, 0.05-0.5 parts of minerals, vitamin
0.03-0.3 parts, 0.01-0.3 parts of nutrient.
2. baby formula milk powder according to claim 1, which is characterized in that the vegetable oil is mixed vegetable oil, institute
Stating mixed vegetable oil includes palm oil, sunflower oil, rapeseed oil, palm-kernel oil, maize oil plus coconut oil, the palm oil, certain herbaceous plants with big flowers
Flower seed oil, rapeseed oil, palm-kernel oil, maize oil plus coconut oil mass ratio be (25-45): (15-40): (15-40): (2-6):
(1-6): (1-5).
3. baby formula milk powder according to claim 1 or 2, which is characterized in that the vitamin include Vitamin D3,
Dl- α-D-α-tocopherol acetate, phytondione, thiamine hydrochloride, riboflavin, puridoxine hydrochloride, cyanocobalamin, niacinamide, D- biology
Element, inositol, folic acid, D-VB5 calcium and taurine；
The Vitamin D3, dl- α-D-α-tocopherol acetate, phytondione, thiamine hydrochloride, riboflavin, puridoxine hydrochloride, cyanogen cobalt
Amine, niacinamide, D-Biotin, inositol, folic acid, D-VB5 calcium and taurine mass ratio be (1-3): (5-25): (0.4-
1.2): (0.1-1): (0.02-0.2): (0.1-0.5): (0.5-1.5): (1-5): (0.003-0.3): (10-25): (0.01-
0.2): (0.3-3): (10-30).
4. baby formula milk powder according to any one of claim 1-3, which is characterized in that the minerals include sulphur
Sour ferrous iron, zinc sulfate, sodium selenite, magnesium sulfate, copper sulphate, manganese sulfate and citric acid, the ferrous sulfate, zinc sulfate, sub- selenium
Sour sodium, magnesium sulfate, copper sulphate, manganese sulfate and citric acid mass ratio be (4-40): (2-10): (0.5-3.5): (40-60):
(0.1-1): (0.02-2): (0.1-1.0).
5. baby formula milk powder described in any one of -4 according to claim 1, which is characterized in that the nucleotide includes
5'-CMP, 5'-monophosphate adenosine, 5 '-creatinine acid disodiums, 5'-GMP2Na and 5'-UMP disodium；
The 5'-CMP, 5'-monophosphate adenosine, 5 '-creatinine acid disodiums, 5'-GMP2Na and 5'-UMP two
The mass ratio of sodium is (10-25): (5-15): (5-15): (2-10): (10-25).
6. baby formula milk powder according to any one of claims 1-5, which is characterized in that the nutrient includes vinegar
Sour retinyl ester, L-AA sodium and lutein, the mass ratio of the retinol acetate, L-AA sodium and lutein are
(1-5): (35-70): (5-20).
7. baby formula milk powder according to claim 1 to 6, which is characterized in that it further include oligofructose,
The parts by weight of the oligofructose are not more than 1 part.
8. a kind of preparation method of baby formula milk powder of any of claims 1-7, includes the following steps:
Desalted whey powder, skimmed milk powder, lactose, galactooligosaccharide, choline chloride, inositol, L-carnitine and nucleotide are dissolved in
In water, the first mixture is obtained；
Vegetable oil is added in first mixture, and carries out homogeneous, obtains the second mixture；
Vitamin and mineral is added into second mixture, and is successively mixed, sterilized, be concentrated and dried, obtains
Base-material；
Lactoferrin, casein phosphopeptide, docosahexaenoic acid, arachidonic acid and nutrient are added into the base-material,
It carries out dry-mixed, obtains the baby formula milk powder.
9. preparation method according to claim 8, which is characterized in that the temperature of the water is 40-65 DEG C, the vegetable oil
Temperature be 40-65 DEG C；
The pressure of the homogeneous is 100-200bar, pressure is 40-65 DEG C；
The temperature of the sterilization is 75-95 DEG C, time 20-90s；
The temperature of the concentration is 70-90 DEG C.
10. preparation method according to claim 8 or claim 9, which is characterized in that the drying successively includes spray drying and stream
It is dry to change bed, 180 DEG C -215 DEG C of the inlet air temperature of the spray drying, 70-90 DEG C of temperature of outgoing air, the fluidized bed drying is successively
Including the Ith dryer section, the IIth dryer section, the IIIth dryer section and Section IV dryer section, the Ith dryer section, the IIth dryer section and
The temperature of III dryer section is 15-95 DEG C, and the temperature of the Section IV dryer section is 15-40 DEG C；
The dry-mixed revolving speed is 10-30r/min, do time >=2min in advance, mixing time 5-10min.
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN113796419A (en) * | 2021-07-05 | 2021-12-17 | 西安百跃羊乳集团有限公司 | Formula sheep milk powder for improving immunity of children and preparation method thereof |
| CN115887625A (en) * | 2022-11-10 | 2023-04-04 | 中国农业大学 | Composition for promoting intestinal repair and preparation method thereof |
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| US6099871A (en) * | 1995-06-01 | 2000-08-08 | Bristol-Myers Squibb Company | Anti-regurgitation infant formula |
| CN104012657A (en) * | 2014-05-21 | 2014-09-03 | 澳优乳业（中国）有限公司 | Amino acid humanized infant formula milk powder and preparation method thereof |
| CN108029768A (en) * | 2017-11-29 | 2018-05-15 | 上海晨冠乳业有限公司 | A kind of baby formula milk powder containing lactoferrin and probiotics and preparation method thereof |
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2018
- 2018-09-11 CN CN201811056272.8A patent/CN109362895A/en active Pending
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| Publication number | Priority date | Publication date | Assignee | Title |
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| US6099871A (en) * | 1995-06-01 | 2000-08-08 | Bristol-Myers Squibb Company | Anti-regurgitation infant formula |
| CN104012657A (en) * | 2014-05-21 | 2014-09-03 | 澳优乳业（中国）有限公司 | Amino acid humanized infant formula milk powder and preparation method thereof |
| CN108029768A (en) * | 2017-11-29 | 2018-05-15 | 上海晨冠乳业有限公司 | A kind of baby formula milk powder containing lactoferrin and probiotics and preparation method thereof |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN113796419A (en) * | 2021-07-05 | 2021-12-17 | 西安百跃羊乳集团有限公司 | Formula sheep milk powder for improving immunity of children and preparation method thereof |
| CN115887625A (en) * | 2022-11-10 | 2023-04-04 | 中国农业大学 | Composition for promoting intestinal repair and preparation method thereof |
| CN115887625B (en) * | 2022-11-10 | 2023-08-11 | 中国农业大学 | Composition for promoting intestinal repair and preparation method thereof |
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