CN109354627A - 一种提高海藻糖水解酶产量的方法 - Google Patents
一种提高海藻糖水解酶产量的方法 Download PDFInfo
- Publication number
- CN109354627A CN109354627A CN201811395710.3A CN201811395710A CN109354627A CN 109354627 A CN109354627 A CN 109354627A CN 201811395710 A CN201811395710 A CN 201811395710A CN 109354627 A CN109354627 A CN 109354627A
- Authority
- CN
- China
- Prior art keywords
- lys
- enzyme
- glu
- trehalose
- gly
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
- C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
- C12N9/2405—Glucanases
- C12N9/2408—Glucanases acting on alpha -1,4-glucosidic bonds
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/70—Vectors or expression systems specially adapted for E. coli
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P19/00—Preparation of compounds containing saccharide radicals
- C12P19/12—Disaccharides
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P19/00—Preparation of compounds containing saccharide radicals
- C12P19/14—Preparation of compounds containing saccharide radicals produced by the action of a carbohydrase (EC 3.2.x), e.g. by alpha-amylase, e.g. by cellulase, hemicellulase
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Y—ENZYMES
- C12Y302/00—Hydrolases acting on glycosyl compounds, i.e. glycosylases (3.2)
- C12Y302/01—Glycosidases, i.e. enzymes hydrolysing O- and S-glycosyl compounds (3.2.1)
- C12Y302/01141—4-Alpha-D-{(1->4)-alpha-D-glucano} trehalose trehalohydrolase (3.2.1.141)
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2319/00—Fusion polypeptide
Landscapes
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Health & Medical Sciences (AREA)
- Zoology (AREA)
- Genetics & Genomics (AREA)
- Wood Science & Technology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Biotechnology (AREA)
- Biochemistry (AREA)
- Microbiology (AREA)
- Biomedical Technology (AREA)
- General Chemical & Material Sciences (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Molecular Biology (AREA)
- Physics & Mathematics (AREA)
- Biophysics (AREA)
- Plant Pathology (AREA)
- Medicinal Chemistry (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Enzymes And Modification Thereof (AREA)
Abstract
Description
Claims (10)
Priority Applications (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
CN201811395710.3A CN109354627B (zh) | 2018-11-22 | 2018-11-22 | 一种提高海藻糖水解酶产量的方法 |
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
CN201811395710.3A CN109354627B (zh) | 2018-11-22 | 2018-11-22 | 一种提高海藻糖水解酶产量的方法 |
Publications (2)
Publication Number | Publication Date |
---|---|
CN109354627A true CN109354627A (zh) | 2019-02-19 |
CN109354627B CN109354627B (zh) | 2020-12-29 |
Family
ID=65338276
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
CN201811395710.3A Active CN109354627B (zh) | 2018-11-22 | 2018-11-22 | 一种提高海藻糖水解酶产量的方法 |
Country Status (1)
Country | Link |
---|---|
CN (1) | CN109354627B (zh) |
Citations (4)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
JPH08116970A (ja) * | 1994-10-18 | 1996-05-14 | Natl Food Res Inst | 高純度糖質関連酵素および固定化糖質関連酵素の製造方法 |
EP1055731A1 (en) * | 1999-05-26 | 2000-11-29 | Kabushiki Kaisha Hayashibara Seibutsu Kagaku Kenkyujo | DNA which encodes trehalase and uses thereof |
CN105039374A (zh) * | 2015-08-10 | 2015-11-11 | 齐鲁工业大学 | 一种淀粉诱导型重组枯草芽孢杆菌及其制备方法与应用 |
CN106754601A (zh) * | 2016-12-21 | 2017-05-31 | 江南大学 | 一种应用磷脂酶c将胞内蛋白在胞外表达的方法 |
-
2018
- 2018-11-22 CN CN201811395710.3A patent/CN109354627B/zh active Active
Patent Citations (4)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
JPH08116970A (ja) * | 1994-10-18 | 1996-05-14 | Natl Food Res Inst | 高純度糖質関連酵素および固定化糖質関連酵素の製造方法 |
EP1055731A1 (en) * | 1999-05-26 | 2000-11-29 | Kabushiki Kaisha Hayashibara Seibutsu Kagaku Kenkyujo | DNA which encodes trehalase and uses thereof |
CN105039374A (zh) * | 2015-08-10 | 2015-11-11 | 齐鲁工业大学 | 一种淀粉诱导型重组枯草芽孢杆菌及其制备方法与应用 |
CN106754601A (zh) * | 2016-12-21 | 2017-05-31 | 江南大学 | 一种应用磷脂酶c将胞内蛋白在胞外表达的方法 |
Non-Patent Citations (2)
Title |
---|
FEESE M D: "Crystal structure of glycosyltrehalose trehalohydrolase from the hyperthermophilic archaeum Sulfolobus solfataricus", 《J.MOL.BIOL》 * |
吴世雄: "嗜酸热硫化叶菌MTSase和MTHase的异源表达及应用", 《中国优秀硕士学位论文全文数据库 工程科技Ⅰ辑》 * |
Also Published As
Publication number | Publication date |
---|---|
CN109354627B (zh) | 2020-12-29 |
Similar Documents
Publication | Publication Date | Title |
---|---|---|
CN107236696B (zh) | 一种表达L.mesenteroides来源的蔗糖磷酸化酶重组枯草芽孢杆菌 | |
CN110628741B (zh) | 麦芽寡糖基海藻糖合成酶突变体及其应用 | |
CN107227304B (zh) | 一种麦芽寡糖基海藻糖合成酶突变体的制备及其应用 | |
CN106929462A (zh) | 一种积累n‑乙酰神经氨酸重组枯草芽孢杆菌及其应用 | |
CN107384902B (zh) | 一种麦芽糖转化率提高的海藻糖合酶及其制备方法和应用 | |
CN109679887A (zh) | 一种利用高效分泌表达的双酶融合酶耦合发酵生产海藻糖的方法 | |
CN109609530A (zh) | 一种海藻糖合成酶及其在海藻糖生产中的应用 | |
CN108753747B (zh) | 一种热稳定性和海藻糖产量提高的MTSase突变体 | |
JP2022512771A (ja) | アオサ多糖リアーゼおよびそのコード遺伝子と応用 | |
CN110055233B (zh) | 一种热稳定性提高的MTSase突变体及其应用 | |
CN113528553B (zh) | 一种密码子优化的n-乙酰氨基葡萄糖转移酶基因及其应用 | |
CN105969713B (zh) | 一种高产麦芽寡糖基海藻糖水解酶的基因工程菌及其应用 | |
CN106434715B (zh) | 麦芽寡糖基海藻糖合成酶及其表达基因与应用 | |
CN109022396A (zh) | 一种酶活提高的α-淀粉酶突变体及其应用 | |
CN108753746B (zh) | 一种热稳定性提高的麦芽寡糖基海藻糖合成酶突变体 | |
CN113308446B (zh) | 一种海藻糖转化率提高的麦芽寡糖基海藻糖合成酶突变体及其应用 | |
CN111172089A (zh) | 一种利用重组海藻糖合成酶合成海藻糖的方法 | |
CN109354627A (zh) | 一种提高海藻糖水解酶产量的方法 | |
CN106520729B (zh) | 麦芽寡糖基海藻糖水解酶及其表达基因与应用 | |
CN109456950A (zh) | 一种环糊精葡萄糖基转移酶的突变体及其应用 | |
CN106754848B (zh) | 一种热稳定性提高的碱性果胶酶突变体 | |
CN109022404A (zh) | 一种新型冷适应性褐藻胶裂解酶AlgA7及其应用 | |
CN109022406A (zh) | 一种具有冷适应特性的褐藻胶裂解酶AlgA1及其应用 | |
CN111944784B (zh) | 热稳定性提高的麦芽寡糖基海藻水解酶突变体及其应用 | |
CN112980762A (zh) | 一种黑曲霉二糖磷酸化酶及其在黑曲霉二糖制备中的应用 |
Legal Events
Date | Code | Title | Description |
---|---|---|---|
PB01 | Publication | ||
PB01 | Publication | ||
SE01 | Entry into force of request for substantive examination | ||
SE01 | Entry into force of request for substantive examination | ||
GR01 | Patent grant | ||
GR01 | Patent grant | ||
TR01 | Transfer of patent right |
Effective date of registration: 20220211 Address after: 421800 room 211-212, zijingfu (building a-4a, Mingang new town), beside National Highway 107, TIYU North Road, Xili neighborhood committee, Wulipai street, Leiyang City, Hengyang City, Hunan Province Patentee after: Hunan Jindai Technology Development Co.,Ltd. Address before: 421800 Building 1, Dongjiang Industrial Park, Leiyang Economic Development Zone, Hunan Province Patentee before: HUNAN HUISHENG BIOTECHNOLOGY Co.,Ltd. |
|
TR01 | Transfer of patent right | ||
PE01 | Entry into force of the registration of the contract for pledge of patent right |
Denomination of invention: A method to increase the production of trehalose hydrolase Effective date of registration: 20221008 Granted publication date: 20201229 Pledgee: Agricultural Bank of China Limited by Share Ltd. Leiyang branch Pledgor: Hunan Jindai Technology Development Co.,Ltd. Registration number: Y2022980017598 |
|
PE01 | Entry into force of the registration of the contract for pledge of patent right |