CN109354521A - A kind of Amino acid liquid fertilizer mother liquor and preparation method thereof - Google Patents
A kind of Amino acid liquid fertilizer mother liquor and preparation method thereof Download PDFInfo
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- CN109354521A CN109354521A CN201811125058.3A CN201811125058A CN109354521A CN 109354521 A CN109354521 A CN 109354521A CN 201811125058 A CN201811125058 A CN 201811125058A CN 109354521 A CN109354521 A CN 109354521A
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05B—PHOSPHATIC FERTILISERS
- C05B7/00—Fertilisers based essentially on alkali or ammonium orthophosphates
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05F—ORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C, e.g. FERTILISERS FROM WASTE OR REFUSE
- C05F17/00—Preparation of fertilisers characterised by biological or biochemical treatment steps, e.g. composting or fermentation
- C05F17/50—Treatments combining two or more different biological or biochemical treatments, e.g. anaerobic and aerobic treatment or vermicomposting and aerobic treatment
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05G—MIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
- C05G5/00—Fertilisers characterised by their form
- C05G5/20—Liquid fertilisers
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02W—CLIMATE CHANGE MITIGATION TECHNOLOGIES RELATED TO WASTEWATER TREATMENT OR WASTE MANAGEMENT
- Y02W30/00—Technologies for solid waste management
- Y02W30/40—Bio-organic fraction processing; Production of fertilisers from the organic fraction of waste or refuse
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Abstract
The present invention discloses a kind of preparation method of Amino acid liquid fertilizer mother liquor comprising step: yeast bacteria agent being added into primary fermentation system, adjusts that pH is 5.0~5.5 and pH is 3.5 or so to carry out two step aerobic fermentations respectively；Filter two step aerobic fermentation objects, obtain yeast mycoprotein filter residue and saccharomycetes to make fermentation filtrate, carbohydrate, inorganic salts and auxiliary material are added into yeast mycoprotein filter residue, it is broken to mix, adjusting pH is 6.5 or so, second order fermentation system is formed, is fermented to second order fermentation Inoculation aspergillus oryzae and bacillus subtilis microbial agent；Aspergillus oryzae and fermentation of bacillus subtilis object are mixed with saccharomycetes to make fermentation filtrate, beer waste liquid is added, adjusting pH is 5.5~6.0, and aerobic fermentation obtains amino acid fermentation object；Amino acid fermentation object is filtered, obtains amino acid fermentation filter residue and amino acid fermentation filtrate, after acidolysis amino acid fermentation filter residue, filters to obtain resolution filtrate, adjusting amino acid fermentation filtrate pH with resolution filtrate is 4.0~4.5；It stands balanced to bubble-free generation.
Description
Technical field
The invention belongs to waste resource utilization technology fields, are related to the resource utilization of food processing by-product, tool
Body is related to a kind of method for preparing Amino acid liquid fertilizer mother liquor by biofermentation approach using food processing by-product.This hair
The bright Amino acid liquid fertilizer mother liquor that further relates to is preparing the application in fertilizer.
Background technique
The food enterprises such as amino acid, sugar and beer by-product or waste production are big, have " ammonia nitrogen height, COD high,
Acidity is low " the features such as, if without reasonably handling consumption, it will huge pressure is caused to environment, in the environmental protection of the new period
Huge cost pressure will be faced under policy will also restrict the capability of sustainable development of enterprise.However, these by-products or useless
Gurry organic nutrition rich in can get good liquid fertilizer by reasonably deploying, fermenting and compound again, not only may be used
To provide good organic fertilizer resource for planting industry, and it can solve the environmental protection pressure of food enterprise.
Summary of the invention
In order to which the environmental protection pressure of food enterprise is effectively relieved, the recycling benefit of food enterprise by-product or waste is realized
A kind of Amino acid liquid fertilizer mother liquor and the preparation method and application thereof is provided with, the present invention.This Amino acid liquid fertilizer mother liquor
Utilize by-product and waste such as monosodium glutamate waste liquid, molasses, beer waste liquid, the bran of oil expression, monosodium glutamate, sugaring and beer producers
Skin etc. is mainly prepared by biofermentation approach by rationally assembling.The Amino acid liquid fertilizer that the present invention is prepared into is female
Liquid contains amino acid, small organic molecule isoreactivity ingredient, and the batch for food processing by-product or waste is innoxious and provides
Sourceization processing provides innoxious and resource utilization approach.
Specifically, the invention provides the following technical scheme: a kind of preparation method of Amino acid liquid fertilizer mother liquor comprising
Step:
Yeast bacteria agent is added into primary fermentation system, adjusts that pH is 5.0~5.5 and pH is 3.5 or so to carry out respectively
Two step aerobic fermentations；
Two step aerobic fermentation objects are filtered, yeast mycoprotein filter residue and saccharomycetes to make fermentation filtrate are obtained, to the saccharomycete
Carbohydrate, inorganic salts and auxiliary material are added in body protein filter residue, are crushed and are mixed, adjusting pH is 6.5 or so, second order fermentation system is formed,
It ferments to second order fermentation Inoculation aspergillus oryzae and bacillus subtilis microbial agent；
Aspergillus oryzae and fermentation of bacillus subtilis object are mixed with the saccharomycetes to make fermentation filtrate, beer waste liquid is added, is adjusted
Saving pH is 5.5~6.0, and aerobic fermentation obtains amino acid fermentation object；
Amino acid fermentation object is filtered, amino acid fermentation filter residue and amino acid fermentation filtrate, the hair of amino acid described in acidolysis are obtained
After ferment filter residue, resolution filtrate is filtered to obtain, adjusting the amino acid fermentation filtrate pH with resolution filtrate is 4.0~4.5；
It stands balanced generate to bubble-free and obtains Amino acid liquid fertilizer mother liquor.
As one of the preferred embodiment of the Amino acid liquid fertilizer mother liquor preparation method, the primary fermentation system includes
Wheat bran suspension slurry, monosodium glutamate waste liquid, sugar waste and inorganic salts；
The wheat bran suspension slurry, monosodium glutamate waste liquid, sugar waste are according to volume ratio 1:(0.8~1.2): (0.3~0.6)
Mixing；
Inorganic salts in the primary fermentation system include ammonium salt, potassium dihydrogen phosphate and selenite, and the ammonium salt adds
Enter amount be the wheat bran suspension slurry, monosodium glutamate waste liquid, sugar waste mixed liquor quality 0.2~0.5%, the potassium dihydrogen phosphate
Additional amount be the wheat bran suspension slurry, monosodium glutamate waste liquid, sugar waste mixed liquor quality 0.2~0.5%, the selenous acid
The additional amount of salt be the wheat bran suspension slurry, monosodium glutamate waste liquid, sugar waste mixed liquor quality 1.5~2.0%.
As one of the preferred embodiment of the Amino acid liquid fertilizer mother liquor preparation method, wherein the wheat bran suspension slurry
Preparation method: by wheat bran and water 1:(1.5~2.0 in mass ratio) ratio mixing be added fermentor, hydrogen is added into fermentor
The additional amount of potassium oxide or sodium hydroxide, the potassium hydroxide or sodium hydroxide is the 2~5% of wheat bran quality, it is heated to 80~
It 85 DEG C, stirs 1~1.5 hour, forms uniform suspension slurry.
As one of the preferred embodiment of the Amino acid liquid fertilizer mother liquor preparation method, in the primary fermentation system
Ammonium salt selects sulphur ammonium or Diammonium phosphate (DAP) or both mixture, and the selenite selects sodium selenite.
As one of the preferred embodiment of the Amino acid liquid fertilizer mother liquor preparation method, the yeast bacteria agent selection gram
This Candida microbial inoculum of Shandong or saccharomyces cerevisiae microbial inoculum or both mixture, the additional amount of the yeast bacteria agent are the primary hair
The 8.0~10.0% of ferment system volume；
1.0~2.0vvm of ventilation ratio of the aerobic fermentation of pH5.0~5.5,28~30 DEG C of temperature, 48~72h of fermentation time, it
PH3.5 or so is adjusted afterwards, continues 24~48h of fermentation.
As one of the preferred embodiment of the Amino acid liquid fertilizer mother liquor preparation method, filtered to the yeast mycoprotein
The carbohydrate added in slag includes sucrose and glucose, and the additional amount of the sucrose is the 5 of the yeast mycoprotein filter residue quality
~8%, the additional amount of the glucose is the 2~5% of the yeast mycoprotein filter residue quality；
The inorganic salts added into the yeast mycoprotein filter residue are potassium nitrate or sodium nitrate, the potassium nitrate or nitric acid
The additional amount of sodium is the 0.1~0.2% of the yeast mycoprotein filter residue quality；
The additional amount of the auxiliary material is the 35~60% of the yeast mycoprotein filter residue quality, the group subpackage of the auxiliary material
Dregs of beans and tapioca are included, content accounting of the dregs of beans in the auxiliary material is 50~60%, and tapioca is in the auxiliary material
Content accounting is 40~50%.
As one of the preferred embodiment of the Amino acid liquid fertilizer mother liquor preparation method, the aspergillus oryzae and withered grass gemma
The inoculum concentration of bacillus microbial inoculum is the 6.0~10.0% of the second order fermentation system quality, the aspergillus oryzae microbial inoculum and withered grass gemma
The volume ratio of bacillus microbial inoculum is 1:1；
The moisture content of the second order fermentation system is adjusted to 60~65%, is inoculated with aspergillus oryzae and bacillus subtilis microbial agent
With a thickness of 20~25cm, fermentation temperature is 26~28 DEG C for fermentation afterwards, and fermentation time is 72~96h.
As one of the preferred embodiment of the Amino acid liquid fertilizer mother liquor preparation method, the additional amount of the beer waste liquid
For the aspergillus oryzae and fermentation of bacillus subtilis object and the 40~60% of the saccharomycetes to make fermentation filtrate mixture volume；Beer
Waste liquid be added fermentation condition be 1.0~2.0vvm of ventilation ratio, 30~33 DEG C of temperature, 48~72h of fermentation time.
As one of the preferred embodiment of the Amino acid liquid fertilizer mother liquor preparation method, the amino acid fermentation filter residue is used
The volume ratio of sulfuric acid and the resolution of phosphoric acid mixed liquor, the amino acid fermentation filter residue and the sulfuric acid and phosphoric acid mixed liquor is 1:
The mass ratio of (0.7~1.0), the sulfuric acid and phosphoric acid is 1:(1~1.5)；
At 130~150 DEG C, pressure is controlled in 1.2~1.5MPa, 1~1.5h of digestion time for acidolysis temperature control；
The standing time for balance is 2~3 days.
On the other hand, the present invention gives a kind of Amino acid liquid fertilizer mother liquor.This Amino acid liquid fertilizer mother liquor is adopted
It is prepared with the method for the invention.
Amino acid liquid fertilizer mother liquor preparation method of the present invention, at least has the following beneficial effects or advantage:
Food processing by-product or waste are utilized the present invention provides a kind of, amino acid is prepared by biofermentation approach
The method of liquid fertilizer mother liquor.This method, which will specifically include, addition monosodium glutamate to give up after wheat bran, clear water, potassium hydroxide stirring and dissolving
Yeast bacteria agent is added after liquid, sugar waste and inorganic salts including sodium selenite, adjust pH to 5.0~5.5 and pH to
3.5 or so carry out two step aerobic fermentations respectively, and filtering obtains filtrate and yeast mycoprotein；By the broken uniformly mixing of fermentation thalli
Glucose, sucrose, potassium nitrate, dregs of beans and tapioca are added after object, adjusting pH is 6.5 or so, is inoculated with aspergillus oryzae and withered grass bud
Then fermentation material crushing is added into above-mentioned yeast ferment filtrate by spore bacillus fermentation, then adding volume ratio is 40~60% beers
Wine waste liquid is adjusted between pH to 6.0~6.5, aerobic fermentation；Filtering fermentation liquor is obtained into filtrate A and filter residue, by filter residue in high temperature
It is cleared up under condition of high voltage with phosphoric acid, sulfuric acid mixture liquid, filtering obtains filtered fluid B；It is 4.0 that used liquor B, which adjusts filtered fluid A to pH,
~4.5；It is to obtain Amino acid liquid fertilizer mother liquor that mixed liquor, which is stood 2~3 days and generated to bubble-free,.
Present invention utilizes numerous food processing byproduct and wastes, are effectively utilized in by-product and waste
The cooperation of carbon source, nitrogen source and thallus is fermented, and the mycoprotein degradation formed using fermentation, which is obtained, improves ammonia in fermentation liquid
The effect of base sour component and organic active substance is conducive to the fertilizer efficiency function of improving prepared Amino acid liquid fertilizer.This hair
The Fertilizer Transformed utilization of bright achievable food processing by-product, waste, realization is turned waste into wealth, while reducing the environment maintenance of enterprise
Cost.
Detailed description of the invention
Fig. 1 is Amino acid liquid fertilizer mother liquor preparation flow schematic diagram described in the present embodiment.
Specific embodiment
Purpose, technical solution and its effect to facilitate the understanding of the present invention, below with reference to embodiment to of the invention
Technical solution is elaborated further.
Embodiment 1
The present embodiment provides a kind of preparation method of Amino acid liquid fertilizer mother liquor comprising following steps:
(1) preparation of wheat bran suspension slurry
By wheat bran and clear water according to mass ratio 1:(1.0~2.0) ratio mixing addition fermentor, it is added into fermentor strong
Alkali inorganic substance, preferably potassium hydroxide or sodium hydroxide, the additive amount of strong basicity inorganic matter are the 2~5% of wheat bran weight, are added
Hot fermentation tank opens stirring to 80~85 DEG C, stirs 1~1.5 hour, uniform suspension slurry is sufficiently stirred into.
(2) mixing of primary fermentation system
Wheat bran suspension slurry, monosodium glutamate waste liquid and sugar waste are separately added into fermentor, wheat bran suspension slurry, monosodium glutamate are useless
Liquid, sugar waste are according to volume ratio 1:(0.8~1.2): (0.3~0.6) mixing stirs and evenly mixs；It is added into fermentor inorganic
Salt, the inorganic salts preferably include ammonium salt, potassium dihydrogen phosphate and selenite.The ammonium salt be preferably sulphur ammonium or Diammonium phosphate (DAP) or
The two mixture, the additional amount of ammonium salt be the wheat bran suspension slurry, monosodium glutamate waste liquid, sugar waste mixed liquor quality 0.2~
0.5%.The additional amount of the potassium dihydrogen phosphate is the wheat bran suspension slurry, monosodium glutamate waste liquid, sugar waste mixed liquor quality
0.2~0.5%.The selenite is preferably sodium selenite, the additional amount of the sodium selenite be the wheat bran suspension slurry,
The 1.5~2.0% of monosodium glutamate waste liquid, sugar waste mixed liquor quality.Above-mentioned each substance is mixed well, primary fermentation body is obtained
System.
(3) two step aerobic fermentations
By between primary fermentation system adjusting pH value to 5.0~5.5, yeast bacteria agent, the ferment are added into fermentor
Female bacteria agent selects candida krusei (Candida krusei) microbial inoculum or saccharomyces cerevisiae (Saccharomyces
Cerevisiae) microbial inoculum or both mixture, the additional amount of the yeast bacteria agent are the primary fermentation system volume
8.0~10.0%, 1.0~2.0vvm of ventilation ratio, 28~30 DEG C of temperature, 48~72h of fermentation time；Extremely with nitre acid for adjusting pH later
3.5 or so, continue 24~48h of fermentation, filtered after two-step fermentation, obtains yeast mycoprotein filter residue and saccharomycetes to make fermentation filtrate.
(4) mixing of second order fermentation system
Gained yeast mycoprotein filter residue is placed in fermentation tank, adds carbohydrate, inorganic salts and auxiliary material respectively, is crushed mixed
It is even, pH to 6.5 or so is adjusted with potassium hydroxide, forms second order fermentation system.The carbohydrate being added in fermentation tank include sucrose and
Glucose, the additional amount of the sucrose are the 5~8% of the yeast mycoprotein filter residue quality, the additional amount of the glucose
It is the 2~5% of the yeast mycoprotein filter residue quality.The inorganic salts being added in fermentation tank are potassium nitrate or sodium nitrate, institute
The additional amount for stating potassium nitrate or sodium nitrate is the 0.1~0.2% of the yeast mycoprotein filter residue quality.It is added in fermentation tank
Auxiliary material component include dregs of beans and tapioca, content accounting of the dregs of beans in the auxiliary material is 50~60%, and tapioca exists
Content accounting in the auxiliary material is 40~50%.The additional amount of auxiliary material be the yeast mycoprotein filter residue quality 35~
60%.
(5) fermentation of second order fermentation system
In second order fermentation Inoculation aspergillus oryzae (Aspergillus oryzae) and bacillus subtilis (Bacillus
Subtilis it) ferments, the inoculum concentration of the aspergillus oryzae and bacillus subtilis microbial agent is the second order fermentation system quality
6.0~10.0%, the volume ratio of the aspergillus oryzae microbial inoculum and bacillus subtilis microbial agent is 1:1.Adjust second order fermentation system
Moisture content is 60~65%, and the fermentation after being inoculated with aspergillus oryzae and bacillus subtilis microbial agent is with a thickness of 20~25cm, fermentation temperature
Degree is 26~28 DEG C, and fermentation time is 72~96h.
(6) preparation of amino acid fermentation object
Aspergillus oryzae and fermentation of bacillus subtilis object (second order fermentation system product) and the saccharomycetes to make fermentation filtrate is mixed
It closes, beer waste liquid is added after mixing, the additional amount of the beer waste liquid is the aspergillus oryzae and fermentation of bacillus subtilis object
With the 40~60% of the saccharomycetes to make fermentation filtrate mixture volume；Adjusting pH with potassium hydroxide is 5.5~6.0, ventilation ratio
1.0~2.0vvm, 30~33 DEG C of temperature, 48~72h of fermentation time.
(7) acidolysis of amino acid fermentation filter residue
Amino acid fermentation object is filtered, amino acid fermentation filter residue and amino acid fermentation filtrate are obtained, by amino acid fermentation filter residue
It is placed in the tank body of high temperature high voltage resistant, acid and alkali-resistance, amino described in the sulfuric acid of addition 4~5mol/L concentration and phosphoric acid mixed liquor acidolysis
The volume ratio of acid fermentation filter residue, the amino acid fermentation filter residue and the sulfuric acid and phosphoric acid mixed liquor is 1:(0.7~1.0), institute
The mass ratio for stating sulfuric acid and phosphoric acid is 1:(1~1.5)；Pressurized, heated resolution, at 130-150 DEG C, pressure control exists digestion condition
1.2-1.5MPa, digestion time 1.5 hours, cold filtration must clear up filtrate, and filter residue next round digests reuse when producing.
(8) balance is stood
Adjusting the amino acid fermentation filtrate pH with resolution filtrate is 4.0~4.5, stands mixed liquor 2~3 days to bubble-free
Generate the Amino acid liquid fertilizer mother liquor for obtaining balance and stability.
Embodiment 2
The present embodiment provides a kind of preparation method of preferred Amino acid liquid fertilizer mother liquor comprising following steps:
(1) it weighs 100kg wheat bran to be placed in fermentor, 150kg clear water, 4kg potassium hydroxide, heating is added into fermentor
It to 85 DEG C, stirs 40 minutes, stands 1 hour, be stirred for 30 minutes, obtain uniform wheat bran suspension slurry.
(2) monosodium glutamate waste liquid 200kg, sugar waste 100kg is added to wheat bran suspension slurry, is sufficiently mixed, adds 2kg
Diammonium phosphate (DAP), 2.5kg potassium dihydrogen phosphate and 10kg sodium selenite mix well and obtain primary fermentation system.
(3) by between primary fermentation system adjusting pH value to 5.0~5.5, Cruise vacation silk ferment well prepared in advance is added
Female microbial inoculum, candida krusei microbial inoculum additional amount are 50L, ventilation ratio 1.5vvm, 28 DEG C of temperature, are cultivated 60 hours, Zhi Houyong
Nitre acid for adjusting pH value continues fermentation 48 hours to 3.5；It is filtered after two-step fermentation, it is (aqueous to obtain yeast mycoprotein filter residue 95kg
Amount is about 60%) and saccharomycetes to make fermentation filtrate about 475L.
(4) yeast mycoprotein filter residue is placed in fermentation tank, sucrose 8kg, glucose 3kg, potassium nitrate 0.2kg, beans is added
Dregs of rice 30kg, tapioca 20kg adjust pH to 6.5 with 1.5mol/L potassium hydroxide solution, are inoculated with aspergillus oryzae and bacillus subtilis
Bacterium mixed bacteria 12L adjusts moisture 60%, and fermentation material ferments 84 hours with a thickness of 25cm, 28 DEG C.
(5) after aspergillus oryzae and fermentation of bacillus subtilis object (second order fermentation system product) object being crushed again with the yeast
The mixing of bacterium ferment filtrate, adds 350L beer waste liquid, adjusts pH to 5.8, ventilation ratio 1.5vvm, temperature 31 using potassium hydroxide
DEG C, it ferments 60 hours, obtains amino acid fermentation object, filter to obtain amino acid fermentation filter residue 125kg (moisture content about 55%) and amino
Acid fermentation filtrate (filtrate A) about 865L.
(6) amino acid fermentation filter residue is placed in the tank body of high temperature high voltage resistant, acid and alkali-resistance, the sulfuric acid of 5mol/L concentration is added
With phosphoric acid mixed liquor (the pure mass ratio of sulfuric acid and phosphoric acid is 1:1) 160kg, at 130~150 DEG C, 1.2~1.4MPa of pressure disappears
Change 1 hour, cold filtration obtains liquor B about 162L and filter residue 48kg (dry about 27kg), and filter residue is returned as when digesting next time
With.
(7) liquor B is added to fermentation liquid A, deploys pH to 4.0~4.2, stand 2~3 days acquisition balance and stabilities of mixed liquor
Amino acid liquid fertilizer mother liquor.
Amino acid liquid fertilizer mother liquor 82.5g/L containing organic matter made from the present embodiment, protein 36.8g/L, free ammonia
Base acid 40.8g/L, P 19.6g/L, K 10.9g/L, N 21.5g/L, selenium 3.8g/L.
Embodiment 3
The present embodiment provides a kind of preparation method of preferred Amino acid liquid fertilizer mother liquor comprising following steps:
(1) it weighs 100kg wheat bran to be placed in fermentor, 180kg clear water, 5kg potassium hydroxide, heating is added into fermentor
It to 85 DEG C, stirs 30 minutes, stands 1 hour, be stirred for 30 minutes, obtain uniform wheat bran suspension slurry.
(2) monosodium glutamate waste liquid 300kg, sugar waste 120kg is added to wheat bran suspension slurry, is sufficiently mixed, adds 3kg
Diammonium phosphate (DAP), 3kg potassium dihydrogen phosphate and 12kg sodium selenite mix well and obtain primary fermentation system.
(3) by between primary fermentation system adjusting pH value to 5.0~5.5, saccharomyces cerevisiae microbial inoculum well prepared in advance is added,
Saccharomyces cerevisiae microbial inoculum additional amount is 60L, ventilation ratio 1.5vvm, 28 DEG C of temperature, cultivates 72 hours, uses nitre acid for adjusting pH value extremely later
3.5, continue fermentation 48 hours；It is filtered after two-step fermentation, obtains yeast mycoprotein filter residue 105kg (water content about 60%) and ferment
Female bacterium ferment filtrate about 615L.
(4) yeast mycoprotein filter residue is placed in fermentation tank, be added sucrose 8.5kg, glucose 3kg, potassium nitrate 0.2kg,
Dregs of beans 28kg, tapioca 20kg adjust pH to 6.5 with 1.5mol/L potassium hydroxide solution, are inoculated with aspergillus oryzae and withered grass gemma
Bacillus mixed bacteria 12L adjusts moisture 60%, and fermentation material ferments 84 hours with a thickness of 25cm, 28 DEG C.
(5) after aspergillus oryzae and fermentation of bacillus subtilis object (second order fermentation system product) object being crushed again with the yeast
The mixing of bacterium ferment filtrate, adds 300L beer waste liquid, adjusts pH to 5.8, ventilation ratio 1.5vvm, temperature 31 using potassium hydroxide
DEG C, it ferments 60 hours, obtains amino acid fermentation object, filter to obtain amino acid fermentation filter residue 113kg (moisture content about 55%) and amino
Acid fermentation filtrate (filtrate A) about 915L.
(6) amino acid fermentation filter residue is placed in the tank body of high temperature high voltage resistant, acid and alkali-resistance, the sulfuric acid of 5mol/L concentration is added
With phosphoric acid mixed liquor (the pure mass ratio of sulfuric acid and phosphoric acid be 1:1.2) 150kg, at 130~150 DEG C, 1.2~1.4MPa of pressure,
Digestion 1.5 hours, cold filtration obtain liquor B about 165L and filter residue 39kg (dry about 23kg), and filter residue is used as to be digested next time
Shi Huiyong.
(7) liquor B is added to fermentation liquid A, deploys pH to 4.0~4.2, stand 2~3 days acquisition balance and stabilities of mixed liquor
Amino acid liquid fertilizer mother liquor.
Amino acid liquid fertilizer mother liquor 78.3g/L containing organic matter made from the present embodiment, protein 34.6g/L, free ammonia
Base acid 42.2g/L, P 18.3g/L, K 10.3g/L, N 20.6g/L, selenium 4.2g/L.
Further narration is done to the present invention above in conjunction with embodiment, but present invention is not limited to the embodiments described above,
Within the knowledge of one of ordinary skill in the art, it can also make without departing from the purpose of the present invention
Various change.
Claims (10)
1. a kind of preparation method of Amino acid liquid fertilizer mother liquor, which is characterized in that comprising steps of
Yeast bacteria agent is added into primary fermentation system, adjusts that pH is 5.0~5.5 and pH is 3.5 or so to carry out two steps respectively
Aerobic fermentation；
Two step aerobic fermentation objects are filtered, yeast mycoprotein filter residue and saccharomycetes to make fermentation filtrate are obtained, to the yeast thallus egg
Carbohydrate, inorganic salts and auxiliary material are added in white filter residue, is crushed and mixes, and adjusting pH is 6.5 or so, second order fermentation system is formed, to two
Aspergillus oryzae and bacillus subtilis microbial agent fermentation are inoculated in grade fermentation system；
Aspergillus oryzae and fermentation of bacillus subtilis object are mixed with the saccharomycetes to make fermentation filtrate, beer waste liquid is added, adjusts pH
It is 5.5~6.0, aerobic fermentation obtains amino acid fermentation object；
Amino acid fermentation object is filtered, amino acid fermentation filter residue and amino acid fermentation filtrate, the filter of amino acid fermentation described in acidolysis are obtained
After slag, resolution filtrate is filtered to obtain, adjusting the amino acid fermentation filtrate pH with resolution filtrate is 4.0~4.5；
It stands balanced generate to bubble-free and obtains Amino acid liquid fertilizer mother liquor.
2. the preparation method of Amino acid liquid fertilizer mother liquor according to claim 1, which is characterized in that the primary fermentation body
System includes wheat bran suspension slurry, monosodium glutamate waste liquid, sugar waste and inorganic salts；
The wheat bran suspension slurry, monosodium glutamate waste liquid, sugar waste are according to volume ratio 1:(0.8~1.2): (0.3~0.6) mixing；
Inorganic salts in the primary fermentation system include ammonium salt, potassium dihydrogen phosphate and selenite, the additional amount of the ammonium salt
For the wheat bran suspension slurry, monosodium glutamate waste liquid, sugar waste mixed liquor quality 0.2~0.5%, the potassium dihydrogen phosphate plus
Enter amount be the wheat bran suspension slurry, monosodium glutamate waste liquid, sugar waste mixed liquor quality 0.2~0.5%, the selenite
Additional amount be the wheat bran suspension slurry, monosodium glutamate waste liquid, sugar waste mixed liquor quality 1.5~2.0%.
3. the preparation method of Amino acid liquid fertilizer mother liquor according to claim 2, which is characterized in that the wheat bran, which suspends, to be starched
The preparation method of liquid: by wheat bran and water 1:(1.5~2.0 in mass ratio) ratio mixing addition fermentor, it is added into fermentor
The additional amount of potassium hydroxide or sodium hydroxide, the potassium hydroxide or sodium hydroxide is the 2~5% of wheat bran quality, is heated to 80
It~85 DEG C, stirs 1~1.5 hour, forms uniform suspension slurry.
4. the preparation method of Amino acid liquid fertilizer mother liquor according to claim 2, which is characterized in that the primary fermentation body
Ammonium salt in system selects sulphur ammonium or Diammonium phosphate (DAP) or both mixture, and the selenite selects sodium selenite.
5. the preparation method of Amino acid liquid fertilizer mother liquor according to claim 1, which is characterized in that the yeast bacteria agent
Candida krusei microbial inoculum or saccharomyces cerevisiae microbial inoculum or both mixture are selected, the additional amount of the yeast bacteria agent is described
The 8.0~10.0% of primary fermentation system volume；
1.0~2.0vvm of ventilation ratio of the aerobic fermentation of pH5.0~5.5,28~30 DEG C of temperature, 48~72h of fermentation time is adjusted later
PH3.5 or so is saved, 24~48h of fermentation is continued.
6. the preparation method of Amino acid liquid fertilizer mother liquor according to claim 1, which is characterized in that the yeast thallus
The carbohydrate added in albumen filter residue includes sucrose and glucose, and the additional amount of the sucrose is the yeast mycoprotein filter residue matter
The 5~8% of amount, the additional amount of the glucose are the 2~5% of the yeast mycoprotein filter residue quality；
The inorganic salts added into the yeast mycoprotein filter residue are potassium nitrate or sodium nitrate, the potassium nitrate or sodium nitrate
Additional amount is the 0.1~0.2% of the yeast mycoprotein filter residue quality；
The additional amount of the auxiliary material is the 35~60% of the yeast mycoprotein filter residue quality, and the component of the auxiliary material includes beans
The dregs of rice and tapioca, content accounting of the dregs of beans in the auxiliary material are 50~60%, content of the tapioca in the auxiliary material
Accounting is 40~50%.
7. the preparation method of Amino acid liquid fertilizer mother liquor according to claim 1, which is characterized in that the aspergillus oryzae and withered
The inoculum concentration of careless gemma bacillus agent is the 6.0~10.0% of the second order fermentation system quality, the aspergillus oryzae microbial inoculum and withered
The volume ratio of careless gemma bacillus agent is 1:1；
The moisture content of the second order fermentation system is adjusted to 60~65%, after being inoculated with aspergillus oryzae and bacillus subtilis microbial agent
With a thickness of 20~25cm, fermentation temperature is 26~28 DEG C for fermentation, and fermentation time is 72~96h.
8. the preparation method of Amino acid liquid fertilizer mother liquor according to claim 1, which is characterized in that the beer waste liquid
Additional amount be the aspergillus oryzae and fermentation of bacillus subtilis object and the saccharomycetes to make fermentation filtrate mixture volume 40~
60%；Beer waste liquid be added fermentation condition be 1.0~2.0vvm of ventilation ratio, 30~33 DEG C of temperature, 48~72h of fermentation time.
9. the preparation method of Amino acid liquid fertilizer mother liquor according to claim 1, which is characterized in that the amino acid fermentation
Filter residue sulfuric acid and the resolution of phosphoric acid mixed liquor, the volume ratio of the amino acid fermentation filter residue and the sulfuric acid and phosphoric acid mixed liquor are
1:(0.7~1.0), the mass ratio of the sulfuric acid and phosphoric acid is 1:(1~1.5)；
At 130~150 DEG C, pressure is controlled in 1.2~1.5MPa, 1~1.5h of digestion time for acidolysis temperature control；
The standing time for balance is 2~3 days.
10. a kind of Amino acid liquid fertilizer mother liquor uses claim 1 the method to prepare.
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| CN111018729A (en) * | 2019-12-03 | 2020-04-17 | 杭州三得农业科技有限公司 | Process for resolving protein and converting protein into amino acid by using sound barrier principle |
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