CN109349644A - Probiotic composition, health food and its preparation method and application - Google Patents
Probiotic composition, health food and its preparation method and application Download PDFInfo
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- CN109349644A CN109349644A CN201811278009.3A CN201811278009A CN109349644A CN 109349644 A CN109349644 A CN 109349644A CN 201811278009 A CN201811278009 A CN 201811278009A CN 109349644 A CN109349644 A CN 109349644A
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- 239000006041 probiotic Substances 0.000 title claims abstract description 121
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- 238000002360 preparation method Methods 0.000 title claims abstract description 31
- 235000013402 health food Nutrition 0.000 title claims abstract description 19
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- 108090000790 Enzymes Proteins 0.000 claims abstract description 24
- 241000894006 Bacteria Species 0.000 claims abstract description 23
- 240000002605 Lactobacillus helveticus Species 0.000 claims description 29
- 235000013967 Lactobacillus helveticus Nutrition 0.000 claims description 29
- 229940054346 lactobacillus helveticus Drugs 0.000 claims description 29
- 240000006024 Lactobacillus plantarum Species 0.000 claims description 28
- 235000013965 Lactobacillus plantarum Nutrition 0.000 claims description 28
- 229940072205 lactobacillus plantarum Drugs 0.000 claims description 28
- 241001608472 Bifidobacterium longum Species 0.000 claims description 26
- 229940009291 bifidobacterium longum Drugs 0.000 claims description 26
- 229920002774 Maltodextrin Polymers 0.000 claims description 14
- 239000005913 Maltodextrin Substances 0.000 claims description 8
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- 239000002778 food additive Substances 0.000 claims description 6
- 235000013373 food additive Nutrition 0.000 claims description 6
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Classifications
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
- A23L33/10—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
- A23L33/135—Bacteria or derivatives thereof, e.g. probiotics
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
- A23L33/10—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
- A23L33/10—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
- A23L33/14—Yeasts or derivatives thereof
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
- A23L33/10—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
- A23L33/16—Inorganic salts, minerals or trace elements
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
- A23L33/20—Reducing nutritive value; Dietetic products with reduced nutritive value
- A23L33/21—Addition of substantially indigestible substances, e.g. dietary fibres
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2002/00—Food compositions, function of food ingredients or processes for food or foodstuffs
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2400/00—Lactic or propionic acid bacteria
- A23V2400/11—Lactobacillus
- A23V2400/147—Helveticus
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2400/00—Lactic or propionic acid bacteria
- A23V2400/11—Lactobacillus
- A23V2400/169—Plantarum
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2400/00—Lactic or propionic acid bacteria
- A23V2400/51—Bifidobacterium
- A23V2400/533—Longum
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Mycology (AREA)
- Chemical & Material Sciences (AREA)
- Health & Medical Sciences (AREA)
- Nutrition Science (AREA)
- Engineering & Computer Science (AREA)
- Food Science & Technology (AREA)
- Polymers & Plastics (AREA)
- Microbiology (AREA)
- Inorganic Chemistry (AREA)
- Medicines Containing Material From Animals Or Micro-Organisms (AREA)
- Coloring Foods And Improving Nutritive Qualities (AREA)
Abstract
The present invention relates to a kind of probiotic compositions, health food and its preparation method and application.A kind of probiotic composition, according to parts by weight, including 4 parts~7 parts of compound probiotics, 2 parts~5 parts of Se-enriched yeast, 5 parts~9 parts of plant enzyme and 45 parts~55 parts of oligofructose, wherein the viable count in the compound probiotic is 2.5 × 1010CFU/g~5.5 × 1010CFU/g.Above-mentioned compound probiotic can supplement beneficial bacteria of intestinal tract, and oligofructose is conducive to the growth and breeding and intestinal health of intestinal beneficial bacterium as prebiotics, and Se-enriched yeast can supplement intracorporal selenium element, and plant enzyme can be improved internal oxidation resistance.By the synergistic effect of compound probiotic, oligofructose, Se-enriched yeast and plant enzyme, and the number of viable in compound probiotic is controlled, above-mentioned probiotic composition can not only improve intestinal microbial balance, but also can significantly improve immunity.
Description
Technical field
The present invention relates to food technology fields, more particularly to a kind of probiotic composition, health food and its preparation side
Method and application.
Background technique
With the raising of living condition, people increasingly pay attention to the health status of oneself.Immunity is the anti-of human body itself
Imperial mechanism is human bioequivalence and any foreign matter (virus, bacterium etc.) for eliminating external intrusion, handles aging, damage, death, change
Property own cells and identification and handle vivo mutations cell and virus infected cell ability.The body of hypoimmunity
Easily incur the infection such as bacterium, virus, fungi, and generates and have a delicate constitution, malnutritive, apathetic, fatigue and weak, appetite
The disease symptoms such as reduction, sleep disturbance, have not only aggravated the consumption of body, and every time it is sick will be lot more time to it is extensive
It is multiple, it can if things go on like this induce major disease.In addition, diet changes, using antibiotic medicine, the variation at age and enteron aisle
Immune dysfunction etc., also will affect the health of body, and lead to enteric flora disturbance, and sensitive intestines bacterium is suppressed, and not pressed down
The bacterium of system and seize the opportunity breeding, so as to cause flora imbalance, make one the symptom for generating constipation.
Summary of the invention
Based on this, it is necessary to which intestinal microbial balance can be improved and improve the probiotic combinations of immunity by providing one kind
Object.
In addition, additionally providing a kind of preparation method and application of probiotic composition, health food and preparation method thereof.
A kind of probiotic composition, according to parts by weight, comprising:
Wherein, the viable count in the compound probiotic is 2.5 × 1010CFU/g~5.5 × 1010CFU/g。
Compound probiotic in above-mentioned probiotic composition can supplement beneficial bacteria of intestinal tract, oligofructose as prebiotics,
Be conducive to the growth and breeding and intestinal health of intestinal beneficial bacterium, Se-enriched yeast can supplement intracorporal selenium element, plant enzyme energy
Enough improve internal oxidation resistance.By the synergistic effect of compound probiotic, oligofructose, Se-enriched yeast and plant enzyme, and
The number of viable in compound probiotic is controlled, above-mentioned probiotic composition can not only improve intestinal microbial balance, but also
Immunity can be significantly improved.
The viable count in the compound probiotic is 3.5 × 10 in one of the embodiments,10CFU/g~4.5 ×
1010CFU/g。
The compound probiotic includes lactobacillus plantarum, Lactobacillus helveticus and long bifid bar in one of the embodiments,
At least one of bacterium.
The compound probiotic includes lactobacillus plantarum, Lactobacillus helveticus and long bifid bar in one of the embodiments,
Bacterium, wherein based on Colony Forming Unit, the viable bacteria of the lactobacillus plantarum and the Lactobacillus helveticus, the bifidobacterium longum
Number is than being 1:4:6~1:6:9.
It in one of the embodiments, further include 21 parts~27 parts of maltodextrin.
It in one of the embodiments, further include 7 parts~13 parts of banaina.
A kind of preparation method of probiotic composition, according to parts by weight, comprising steps of by 4 parts~7 parts of compound benefit
Raw bacterium, 2 parts~5 parts of Se-enriched yeast, 5 parts~9 parts of plant enzyme and the mixing of 45 parts~55 parts of oligofructose, obtain benefit
Raw bacteria composition, wherein the viable count in the compound probiotic is 2.5 × 1010CFU/g~5.5 × 1010CFU/g。
Probiotic composition made from the preparation method of above-mentioned probiotic composition or above-mentioned probiotic composition exists
Prepare the application in health food.
A kind of health food, the preparation method system including above-mentioned probiotic composition or above-mentioned probiotic composition
The probiotic composition and food additives obtained.
A kind of preparation method of health food, comprising steps of
By probiotic composition made from the preparation method of above-mentioned probiotic composition or above-mentioned probiotic composition
It is mixed with food additives, obtains health food.
Specific embodiment
The invention can be realized in many different forms, however it is not limited to embodiment described herein.On the contrary,
Purpose of providing these embodiments is makes the disclosure of the present invention more thorough and comprehensive.
Unless otherwise defined, all technical and scientific terms used herein and belong to technical field of the invention
The normally understood meaning of technical staff it is identical.Term as used herein in the specification of the present invention is intended merely to retouch
State the purpose of specifically embodiment, it is not intended that in the limitation present invention.
The probiotic composition of one embodiment, according to parts by weight, comprising:
Wherein, the viable count in compound probiotic is 2.5 × 1010CFU/g~5.5 × 1010CFU/g.Further, multiple
Closing the viable count in probiotics is 3.5 × 1010CFU/g~4.5 × 1010CFU/g.Specifically, the mass parts of compound probiotic
Number is 5.67 parts.Specifically, compound probiotic is freeze-dried powder.
Further, compound probiotic includes at least one in lactobacillus plantarum, Lactobacillus helveticus and bifidobacterium longum
Kind.Wherein, lactobacillus plantarum is lactobacillus plantarum R-1012；Lactobacillus helveticus is Lactobacillus helveticus R-52；Bifidobacterium longum is
Bifidobacterium longum R-175.
Further, compound probiotic includes lactobacillus plantarum, Lactobacillus helveticus and bifidobacterium longum, wherein presses bacterium
It falls to form unit meter, the viable count ratio of lactobacillus plantarum and Lactobacillus helveticus, bifidobacterium longum is 1:4:6~1:6:9.Specifically
The viable count ratio of ground, lactobacillus plantarum and Lactobacillus helveticus, bifidobacterium longum is 1:4.7:7.3.
Wherein, Se-enriched yeast can supplement intracorporal selenium element.Further, the mass fraction of Se-enriched yeast is 3.33
Part.Specifically, Se-enriched yeast is Se-enriched yeast powder.
Wherein, plant enzyme contains various ferment, oligosaccharides and a variety of vitamins and minerals of human body needs, and can produce
Raw SOD antioxidase ingredient abundant, improves internal oxidation resistance, and then strengthen immunity.Further, plant enzyme
Mass fraction be 7 parts.Specifically, plant enzyme is vegetables and fruits plant enzyme powder.
Wherein, oligofructose is conducive to the growth and breeding and intestinal health of intestinal beneficial bacterium as prebiotics.Further
Ground, the mass fraction of oligofructose are 50 parts.
It should be noted that above-mentioned probiotic composition further includes 21 parts~27 parts of maltodextrin.Maltodextrin can
More easily absorb probiotic composition.Further, the mass fraction of maltodextrin is 24 parts.
It should be noted that above-mentioned probiotic composition further includes 7 parts~13 parts of banaina.Banaina can improve
The mouthfeel of probiotic composition.Further, the mass fraction of banaina is 10 parts.
Above-mentioned probiotic composition at least has the advantages that
1) compound probiotic in above-mentioned probiotic composition can supplement beneficial bacteria of intestinal tract, and oligofructose is as prebiotic
Member, is conducive to the growth and breeding and intestinal health of intestinal beneficial bacterium, and Se-enriched yeast can supplement intracorporal selenium element, plant ferment
Element can be improved internal oxidation resistance.Made by the collaboration of compound probiotic, oligofructose, Se-enriched yeast and plant enzyme
With, and the number of viable in compound probiotic is controlled, above-mentioned probiotic composition can not only improve intestinal microbial balance,
But also immunity can be significantly improved.
2) maltodextrin and banaina be also added in above-mentioned probiotic composition, and makes the mouthfeel of probiotic composition
Good, nutritional ingredient is easy to absorb.
The preparation method of the probiotic composition of one embodiment is one of preparation of above-mentioned probiotic composition
Method, according to parts by weight, comprising the following steps:
By 4 parts~7 parts of compound probiotic, 2 parts~5 parts of Se-enriched yeast, 5 parts~9 parts of plant enzyme and 45 parts~
55 parts of oligofructose mixing, obtains probiotic composition, wherein and viable count in the compound probiotic is 2.5 ×
1010CFU/g~5.5 × 1010CFU/g。
Wherein, compound probiotic is freeze-dried powder.Further, the viable count in compound probiotic is 3.5 × 1010 CFU/g
~4.5 × 1010CFU/g.Specifically, the mass fraction of compound probiotic is 5.67 parts.
Further, compound probiotic includes at least one in lactobacillus plantarum, Lactobacillus helveticus and bifidobacterium longum
Kind.Wherein, lactobacillus plantarum is lactobacillus plantarum R-1012；Lactobacillus helveticus is Lactobacillus helveticus R-52；Bifidobacterium longum is
Bifidobacterium longum R-175.
Further, compound probiotic includes lactobacillus plantarum, Lactobacillus helveticus and bifidobacterium longum, wherein presses bacterium
It falls to form unit meter, the viable count ratio of lactobacillus plantarum and Lactobacillus helveticus, bifidobacterium longum is 1:4:6~1:6:9.Specifically
The viable count ratio of ground, lactobacillus plantarum and Lactobacillus helveticus, bifidobacterium longum is 1:4.7:7.3.
Further, the mass fraction of Se-enriched yeast is 3.33 parts.Specifically, Se-enriched yeast is Se-enriched yeast powder.
Further, the mass fraction of plant enzyme is 7 parts.Specifically, plant enzyme is vegetables and fruits plant enzyme powder.
Further, the mass fraction of oligofructose is 50 parts.
Further, by 4 parts~7 parts of compound probiotic, 2 parts~5 parts of Se-enriched yeast, 5 parts~9 parts of plant ferment
It further include by 4 parts~7 parts of compound probiotic, 2 parts~5 parts before the step of element and the mixing of 45 parts~55 parts of oligofructose
Se-enriched yeast, the step of oligofructose of 5 parts~9 parts of plant enzyme and 45 parts~55 parts is dry, sieving.
Specifically, dry step are as follows: under conditions of 50 DEG C~70 DEG C, by 4 parts~7 parts of compound probiotic, 2 parts
~5 parts of Se-enriched yeast, 5 parts~9 parts of plant enzyme and 45 parts~55 parts of oligofructose drying to moisture content are lower than
3%.
Specifically, in the step of sieving, the mesh number of sieve is 50 mesh~70 mesh.
It should be noted that by 4 parts~7 parts of compound probiotic, 2 parts~5 parts of Se-enriched yeast, 5 parts~9 parts of plant
It further include the maltodextrin that joined 21 parts~27 parts in the step of object ferment and the mixing of 45 parts~55 parts of oligofructose.Into
One step, the mass fraction of maltodextrin is 24 parts.
It should be noted that by 4 parts~7 parts of compound probiotic, 2 parts~5 parts of Se-enriched yeast, 5 parts~9 parts of plant
It further include the banaina that joined 7 parts~13 parts in the step of object ferment and the mixing of 45 parts~55 parts of oligofructose.Into one
Step ground, the mass fraction of banaina are 10 parts.
The preparation method of above-mentioned probiotic composition is simple to operation, is suitable for industrialized production.
Probiotic composition made from the preparation method of above-mentioned probiotic composition or above-mentioned probiotic composition exists
Prepare the application in health food.For example, preparing the application in selenium-enriched food.
The health food of one embodiment includes the preparation of above-mentioned probiotic composition or above-mentioned probiotic composition
Probiotic composition made from method and food additives.The health food can be lozenge, chewable tablets or solid beverage, no
But there is defaecation, improve the effect of immunity, and in good taste, nutritional ingredient is easy to absorb.
The preparation method of the health food of one embodiment, comprising steps of
By probiotic composition made from the preparation method of above-mentioned probiotic composition or above-mentioned probiotic composition
It is mixed with food additives, obtains health food.The preparation method of the health food is simple to operation, is suitable for industrialized production.
The following are specific embodiment parts:
Embodiment 1
The probiotic composition of the present embodiment the preparation method is as follows:
1) 0.43 part of lactobacillus plantarum R1012,2.02 parts of Lactobacillus helveticus R-52,3.22 parts according to parts by weight, are weighed
Bifidobacterium longum R-175,3.33 parts of Se-enriched yeast powder, 7 parts of vegetables and fruits plant enzyme powder, 50 parts of oligofructose, 24 portions of maltodextrins
And the raw materials such as 10 portions of banainas, wherein the work of lactobacillus plantarum R-1012, Lactobacillus helveticus R-52 and bifidobacterium longum R-175
Bacterium number is 3.5 × 1010CFU/g~4.5 × 1010CFU/g；
2) above-mentioned raw materials are dried under conditions of 60 DEG C to moisture content and is lower than 3%, be then sieved, be uniformly mixed, obtain
To probiotic composition, wherein the mesh number of sieve is 60 mesh.
Embodiment 2
The probiotic composition of the present embodiment the preparation method is as follows:
1) 0.43 part of lactobacillus plantarum R1012,2.02 parts of Lactobacillus helveticus R-52,3.22 parts according to parts by weight, are weighed
Bifidobacterium longum R-175,2.5 parts of Se-enriched yeast powder, 7 parts of vegetables and fruits plant enzyme powder, 55 parts of oligofructose, 19.83 parts of malt pastes
Essence and the raw materials such as 10 portions of banainas, wherein lactobacillus plantarum R-1012, Lactobacillus helveticus R-52 and bifidobacterium longum R-175's
Viable count is 3.5 × 1010CFU/g~4.5 × 1010CFU/g；
2) above-mentioned raw materials are dried under conditions of 60 DEG C to moisture content and is lower than 3%, be then sieved, be uniformly mixed, obtain
To probiotic composition, wherein the mesh number of sieve is 60 mesh.
Embodiment 3
The probiotic composition of the present embodiment the preparation method is as follows:
1) 0.3 part of lactobacillus plantarum R1012,1.7 parts of Lactobacillus helveticus R-52,2 parts long couple according to parts by weight, are weighed
Discrimination bacillus R-175,2 parts of Se-enriched yeast powder, 5 parts of vegetables and fruits plant enzyme powder, 45 parts of oligofructose, 21 portions of maltodextrins and 7 parts of perfume (or spice)
The raw materials such as any of several broadleaf plants powder, wherein the viable count of lactobacillus plantarum R-1012, Lactobacillus helveticus R-52 and bifidobacterium longum R-175 is
2.5×1010CFU/g~5.5 × 1010CFU/g；
2) above-mentioned raw materials are dried under conditions of 50 DEG C to moisture content and is lower than 3%, be then sieved, be uniformly mixed, obtain
To probiotic composition, wherein the mesh number of sieve is 50 mesh.
Embodiment 4
The probiotic composition of the present embodiment the preparation method is as follows:
1) 0.5 part of lactobacillus plantarum R1012,2.5 parts of Lactobacillus helveticus R-52,4 parts long couple according to parts by weight, are weighed
Discrimination bacillus R-175,5 parts of Se-enriched yeast powder, 9 parts of vegetables and fruits plant enzyme powder, 55 parts of oligofructose, 27 portions of maltodextrins and 13 parts
The raw materials such as banaina, wherein the viable count of lactobacillus plantarum R-1012, Lactobacillus helveticus R-52 and bifidobacterium longum R-175 are equal
It is 3.5 × 1010CFU/g~4.5 × 1010CFU/g；
2) above-mentioned raw materials are dried under conditions of 70 DEG C to moisture content and is lower than 3%, be then sieved, be uniformly mixed, obtain
To probiotic composition, wherein the mesh number of sieve is 70 mesh.
Comparative example 1
The probiotic composition of this comparative example the preparation method is as follows:
1) according to parts by weight, weigh 3.33 parts of Se-enriched yeast powder, 7 parts of vegetables and fruits plant enzyme powder, 50 parts of oligofructose,
The raw materials such as 29.67 portions of maltodextrins and 10 portions of banainas；
2) above-mentioned raw materials are dried under conditions of 60 DEG C to moisture content and is lower than 3%, be then sieved, be uniformly mixed, obtain
To probiotic composition, wherein the mesh number of sieve is 60 mesh.
Comparative example 2
The probiotic composition of this comparative example the preparation method is as follows:
1) 0.43 part of lactobacillus plantarum R1012,2.02 parts of Lactobacillus helveticus R-52,3.22 parts according to parts by weight, are weighed
Bifidobacterium longum R-175,7 parts of vegetables and fruits plant enzyme powder, 50 parts of oligofructose, 27.33 portions of maltodextrins and 10 portions of banainas etc.
Raw material, wherein the viable count of lactobacillus plantarum R-1012, Lactobacillus helveticus R-52 and bifidobacterium longum R-175 is 3.5 ×
1010CFU/g~4.5 × 1010CFU/g；
2) above-mentioned raw materials are dried under conditions of 60 DEG C to moisture content and is lower than 3%, be then sieved, be uniformly mixed, obtain
To probiotic composition, wherein the mesh number of sieve is 60 mesh.
Comparative example 3
The probiotic composition of this comparative example the preparation method is as follows:
1) 0.43 part of lactobacillus plantarum R1012,2.02 parts of Lactobacillus helveticus R-52,3.22 parts according to parts by weight, are weighed
The originals such as bifidobacterium longum R-175,3.33 parts of Se-enriched yeast powder, 50 parts of oligofructose, 24 portions of maltodextrins and 10 portions of banainas
Material, wherein the viable count of lactobacillus plantarum R-1012, Lactobacillus helveticus R-52 and bifidobacterium longum R-175 is 3.5 ×
1010CFU/g~4.5 × 1010CFU/g；
2) above-mentioned raw materials are dried under conditions of 60 DEG C to moisture content and is lower than 3%, be then sieved, be uniformly mixed, obtain
To probiotic composition, wherein the mesh number of sieve is 60 mesh.
Immune function test:
(1) experimental animal grouping and administration
Choose a certain amount of age of mouse 2 months or so, the Kunming mouse of 20 ± 2g of weight.By mouse be randomly divided into blank group,
Test group and control group, wherein test group is divided into five groups, i.e. test group 1, test group 2, test group 3, test group 4 and test group
5, every group mouse 20.
Blank group gives 37 DEG C of warm water stomach-fillings 3 times daily；Test group 1 is daily using probiotic combinations made from embodiment 1
Object solution stomach-filling 3 times, the composition solution concentration is 100mg/kg, to be prepared in 37 DEG C of warm waters, each stomach-filling
0.5mL；Test group 2 uses made from embodiment 2 stomach-filling of probiotic composition solution 3 times daily, and the composition solution concentration is
100mg/kg, to be prepared in 37 DEG C of warm waters, each stomach-filling 0.5mL；Test group 3 is daily using benefit made from comparative example 1
Raw the stomach-filling of bacteria composition solution 3 times, the composition solution concentration is 100mg/kg, to be prepared in 37 DEG C of warm waters, every time
Stomach-filling 0.5mL；Test group 4 uses made from comparative example 2 stomach-filling of probiotic composition solution 3 times daily, the composition solution
Amount is 100mg/kg, to be prepared in 37 DEG C of warm waters, each stomach-filling 0.5mL；Test group 5 is made using comparative example 3 daily
The stomach-filling of probiotic composition solution 3 times obtained, the composition solution dosage is 100mg/kg, to prepare in 37 DEG C of warm waters
At each stomach-filling 0.5mL；According to the literature, vitamin A and its reactive derivative play a significant role in immunological regulation, both
Influence cellular immunity influences humoral immunity again, therefore control group gives mouse vitamin A 100mg/kg daily, point 3 stomach-fillings.
(2) mouse peripheral blood immunocyte and peritoneal macrophage measurement
18th day, the eyeball of mouse of blank group, test group and control group is won respectively, acquires whole peripheral bloods (with mouse
Haemorrhagic shock is standard), after the hypotonic destruction red blood cell of Tris-NH4CI solution, immunocytochemistry is carried out, as a result such as table
Shown in 1.The then mouse on clean bench after dissection blood sampling, takes peritoneal macrophage to be counted, as a result such as 1 institute of table
Show.
Table 1
As can be seen from Table 1, the quantity of the mouse peripheral blood immunocyte, peritoneal macrophage of test group 1 and 2 is than sky
White group and control group dramatically increase (P < 0.05), the mouse peripheral blood immunocyte of test group 3~5, peritoneal macrophage
Quantity increased than blank group and control group, but the mouse peripheral blood immunocyte of considerably less than test group 1 and 2, abdominal cavity
The quantity of macrophage.As it can be seen that compared with comparative example 1~3, probiotic composition made from Examples 1 and 2 can more added with
The immunity of the raising mouse of effect.
(3) mouse spleen lymphocyte conversion test
By the spleen of the mouse after above-mentioned dissection blood sampling, it is 1 × 10 that cell concentration, which is made,7The splenocyte suspension of/mL, adds
Entering proleulzin (IL-2) makes its final concentration of 200IU/mL, and concanavalin A (ConA), which is added, makes its final concentration be respectively 5 μ g/
Cell liquid is laid in 96 holes with 200 holes μ L/ amount and is cultivated by mL, after 48h, activates feelings with mtt assay detection splenic lymphocytes
Condition.
(4) mouse natural killer cells (NK cell) determination of activity
YAC-1 cell for 24 hours after taking above-mentioned splenocyte suspension (effector cell) to pass on, it is 1 × 10 that cell concentration, which is made,5/
ML solution (target cell) takes each 100 μ L of effector cell, target cell to be added in 96 well culture plates, and target cell Spontaneous release hole is added
Target cell and each 100 μ L of 1% NP40 is added in target cell and each 100 μ L of culture solution, maximum relief hole, and items are done 3 respectively and put down
Row.After cultivating 4h, 1500r/min is centrifuged 5min.100 μ L supernatants are drawn in 96 hole flat-bottomed plates in every hole, are added simultaneously
100 μ L LDH matrix, react 3min, and every hole is added the HCl of 30 μ L 1mol/L, measures optical density at 490nm with microplate reader
It is worth (OD value).
Table 2
| Group | Mouse quantity (only) | Splenic lymphocytes activate OD570 | NK cell activity OD570 |
| Blank group | 20 | 0.12±0.02 | 42±5.3 |
| Test group 1 | 20 | 0.27±0.01 | 54±3.5 |
| Test group 2 | 20 | 0.25±0.05 | 51±4.6 |
| Test group 3 | 20 | 0.20±0.03 | 48±3.7 |
| Test group 4 | 20 | 0.13±0.04 | 43±4.1 |
| Test group 5 | 20 | 0.13±0.03 | 45±4.0 |
| Control group | 20 | 0.10±0.03 | 40±3.1 |
From table 2 it can be seen that the quantity of the mouse spleen lymphocyte activation number of test group 1 and 2 is than blank group and control
Group dramatically increases (P < 0.05), and NK cell activity significantly improves (P < 0.01), the splenic lymphocytes of the mouse of test group 3~5
The quantity of activation number increased than blank group and positive controls, and NK cell activity also increases, but effect is obviously not
Such as test group 1~2.As it can be seen that probiotic composition made from Examples 1 and 2 can be more efficient compared with comparative example 1~3
Raising mouse immunity.
Each technical characteristic of embodiment described above can be combined arbitrarily, for simplicity of description, not to above-mentioned reality
It applies all possible combination of each technical characteristic in example to be all described, as long as however, the combination of these technical characteristics is not deposited
In contradiction, all should be considered as described in this specification.
The embodiments described above only express several embodiments of the present invention, and the description thereof is more specific and detailed, but simultaneously
It cannot therefore be construed as limiting the scope of the patent.It should be pointed out that coming for those of ordinary skill in the art
It says, without departing from the inventive concept of the premise, various modifications and improvements can be made, these belong to guarantor of the invention
Protect range.Therefore, the scope of protection of the patent of the invention shall be subject to the appended claims.
Claims (10)
1. a kind of probiotic composition, which is characterized in that according to parts by weight, comprising:
Wherein, the viable count in the compound probiotic is 2.5 × 1010CFU/g~5.5 × 1010CFU/g。
2. probiotic composition according to claim 1, which is characterized in that the viable count in the compound probiotic is
3.5×1010CFU/g~4.5 × 1010CFU/g。
3. probiotic composition according to claim 1, which is characterized in that the compound probiotic includes plant cream bar
At least one of bacterium, Lactobacillus helveticus and bifidobacterium longum.
4. probiotic composition according to claim 3, which is characterized in that the compound probiotic includes plant cream bar
Bacterium, Lactobacillus helveticus and bifidobacterium longum, wherein based on Colony Forming Unit, the lactobacillus plantarum and Switzerland's cream bar
Bacterium, the bifidobacterium longum viable count ratio be 1:4:6~1:6:9.
5. probiotic composition according to claim 1, which is characterized in that further include 21 parts~27 parts of maltodextrin.
6. probiotic composition according to claim 1, which is characterized in that further include 7 parts~13 parts of banaina.
7. a kind of preparation method of probiotic composition, which is characterized in that according to parts by weight, comprising steps of by 4 parts~7 parts
Compound probiotic, 2 parts~5 parts of Se-enriched yeast, 5 parts~9 parts of plant enzyme and 45 parts~55 parts oligofructose mixing,
Obtain probiotic composition, wherein the viable count in the compound probiotic is 2.5 × 1010CFU/g~5.5 × 1010CFU/
g。
8. the preparation of the described in any item probiotic compositions of claim 1~6 or probiotic composition as claimed in claim 7
Probiotic composition made from method is preparing the application in health food.
9. a kind of health food, which is characterized in that including the described in any item probiotic compositions of claim 1~6 or right
It is required that probiotic composition made from the preparation method of probiotic composition described in 7 and food additives.
10. a kind of preparation method of health food, which is characterized in that comprising steps of
By the preparation of the described in any item probiotic compositions of claim 1~6 or probiotic composition as claimed in claim 7
Probiotic composition made from method is mixed with food additives, obtains health food.
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