CN109349172A - A kind of loach and Misgurnus auguillicaudatus cross breeding method - Google Patents
A kind of loach and Misgurnus auguillicaudatus cross breeding method Download PDFInfo
- Publication number
- CN109349172A CN109349172A CN201811472997.5A CN201811472997A CN109349172A CN 109349172 A CN109349172 A CN 109349172A CN 201811472997 A CN201811472997 A CN 201811472997A CN 109349172 A CN109349172 A CN 109349172A
- Authority
- CN
- China
- Prior art keywords
- loach
- water
- pond
- mus
- dosage
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
- 241000252185 Cobitidae Species 0.000 title claims abstract description 126
- 238000000034 method Methods 0.000 title claims abstract description 30
- 241001275944 Misgurnus anguillicaudatus Species 0.000 title claims abstract description 26
- 238000009402 cross-breeding Methods 0.000 title claims abstract description 10
- 230000012447 hatching Effects 0.000 claims abstract description 18
- 230000004720 fertilization Effects 0.000 claims abstract description 8
- 230000000384 rearing effect Effects 0.000 claims abstract description 7
- 230000009027 insemination Effects 0.000 claims abstract description 6
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 67
- 235000013336 milk Nutrition 0.000 claims description 29
- 239000008267 milk Substances 0.000 claims description 29
- 210000004080 milk Anatomy 0.000 claims description 29
- 239000000843 powder Substances 0.000 claims description 27
- 244000068988 Glycine max Species 0.000 claims description 23
- 235000010469 Glycine max Nutrition 0.000 claims description 23
- 102000002322 Egg Proteins Human genes 0.000 claims description 18
- 108010000912 Egg Proteins Proteins 0.000 claims description 18
- 210000004681 ovum Anatomy 0.000 claims description 18
- 239000005906 Imidacloprid Substances 0.000 claims description 15
- 235000013601 eggs Nutrition 0.000 claims description 15
- YWTYJOPNNQFBPC-UHFFFAOYSA-N imidacloprid Chemical compound [O-][N+](=O)\N=C1/NCCN1CC1=CC=C(Cl)N=C1 YWTYJOPNNQFBPC-UHFFFAOYSA-N 0.000 claims description 15
- 229940056881 imidacloprid Drugs 0.000 claims description 15
- ODINCKMPIJJUCX-UHFFFAOYSA-N Calcium oxide Chemical compound [Ca]=O ODINCKMPIJJUCX-UHFFFAOYSA-N 0.000 claims description 14
- 238000004659 sterilization and disinfection Methods 0.000 claims description 10
- 239000007924 injection Substances 0.000 claims description 9
- 238000002347 injection Methods 0.000 claims description 9
- 241001597062 Channa argus Species 0.000 claims description 7
- 239000000292 calcium oxide Substances 0.000 claims description 7
- 235000012255 calcium oxide Nutrition 0.000 claims description 7
- -1 compound amino acid Chemical class 0.000 claims description 7
- 238000000855 fermentation Methods 0.000 claims description 7
- 230000004151 fermentation Effects 0.000 claims description 7
- 239000003814 drug Substances 0.000 claims description 6
- 210000002969 egg yolk Anatomy 0.000 claims description 6
- 210000003746 feather Anatomy 0.000 claims description 6
- 210000003608 fece Anatomy 0.000 claims description 6
- 239000010871 livestock manure Substances 0.000 claims description 6
- 210000003205 muscle Anatomy 0.000 claims description 6
- 230000008569 process Effects 0.000 claims description 5
- 241000251468 Actinopterygii Species 0.000 claims description 4
- 235000019688 fish Nutrition 0.000 claims description 4
- 239000007921 spray Substances 0.000 claims description 4
- 230000001954 sterilising effect Effects 0.000 claims description 4
- CPKVUHPKYQGHMW-UHFFFAOYSA-N 1-ethenylpyrrolidin-2-one;molecular iodine Chemical compound II.C=CN1CCCC1=O CPKVUHPKYQGHMW-UHFFFAOYSA-N 0.000 claims description 3
- 229920000153 Povidone-iodine Polymers 0.000 claims description 3
- 210000001015 abdomen Anatomy 0.000 claims description 3
- 238000009395 breeding Methods 0.000 claims description 3
- 230000001488 breeding effect Effects 0.000 claims description 3
- 150000001875 compounds Chemical class 0.000 claims description 3
- 201000010099 disease Diseases 0.000 claims description 3
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 claims description 3
- 229940079593 drug Drugs 0.000 claims description 3
- 229940046085 endocrine therapy drug gonadotropin releasing hormone analogues Drugs 0.000 claims description 3
- XLXSAKCOAKORKW-AQJXLSMYSA-N gonadorelin Chemical class C([C@@H](C(=O)NCC(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](CCCNC(N)=N)C(=O)N1[C@@H](CCC1)C(=O)NCC(N)=O)NC(=O)[C@H](CO)NC(=O)[C@H](CC=1C2=CC=CC=C2NC=1)NC(=O)[C@H](CC=1N=CNC=1)NC(=O)[C@H]1NC(=O)CC1)C1=CC=C(O)C=C1 XLXSAKCOAKORKW-AQJXLSMYSA-N 0.000 claims description 3
- 230000007935 neutral effect Effects 0.000 claims description 3
- 244000045947 parasite Species 0.000 claims description 3
- 239000002504 physiological saline solution Substances 0.000 claims description 3
- 229920003023 plastic Polymers 0.000 claims description 3
- 239000004033 plastic Substances 0.000 claims description 3
- 229960001621 povidone-iodine Drugs 0.000 claims description 3
- 238000004886 process control Methods 0.000 claims description 3
- 108010044781 salmon gonadotropin Proteins 0.000 claims description 3
- 239000002689 soil Substances 0.000 claims description 3
- 238000003756 stirring Methods 0.000 claims description 3
- 238000003860 storage Methods 0.000 claims description 3
- 230000003442 weekly effect Effects 0.000 claims description 3
- 239000006185 dispersion Substances 0.000 claims description 2
- 239000010802 sludge Substances 0.000 claims description 2
- 230000037396 body weight Effects 0.000 claims 1
- 230000018109 developmental process Effects 0.000 claims 1
- 230000004083 survival effect Effects 0.000 abstract description 13
- 238000009360 aquaculture Methods 0.000 abstract description 3
- 244000144974 aquaculture Species 0.000 abstract description 3
- 238000007726 management method Methods 0.000 description 8
- 238000005516 engineering process Methods 0.000 description 3
- KAESVJOAVNADME-UHFFFAOYSA-N Pyrrole Chemical compound C=1C=CNC=1 KAESVJOAVNADME-UHFFFAOYSA-N 0.000 description 2
- SMWDFEZZVXVKRB-UHFFFAOYSA-N Quinoline Chemical compound N1=CC=CC2=CC=CC=C21 SMWDFEZZVXVKRB-UHFFFAOYSA-N 0.000 description 2
- 235000008733 Citrus aurantifolia Nutrition 0.000 description 1
- 241000252184 Misgurnus Species 0.000 description 1
- 244000131316 Panax pseudoginseng Species 0.000 description 1
- 235000005035 Panax pseudoginseng ssp. pseudoginseng Nutrition 0.000 description 1
- 235000003140 Panax quinquefolius Nutrition 0.000 description 1
- OAICVXFJPJFONN-UHFFFAOYSA-N Phosphorus Chemical compound [P] OAICVXFJPJFONN-UHFFFAOYSA-N 0.000 description 1
- 208000035199 Tetraploidy Diseases 0.000 description 1
- 235000011941 Tilia x europaea Nutrition 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 230000008901 benefit Effects 0.000 description 1
- 230000008859 change Effects 0.000 description 1
- 238000004140 cleaning Methods 0.000 description 1
- 238000007796 conventional method Methods 0.000 description 1
- 235000008434 ginseng Nutrition 0.000 description 1
- PCHJSUWPFVWCPO-UHFFFAOYSA-N gold Chemical compound [Au] PCHJSUWPFVWCPO-UHFFFAOYSA-N 0.000 description 1
- 239000010931 gold Substances 0.000 description 1
- 229910052737 gold Inorganic materials 0.000 description 1
- 230000036541 health Effects 0.000 description 1
- 238000009396 hybridization Methods 0.000 description 1
- 239000004571 lime Substances 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- 230000013011 mating Effects 0.000 description 1
- 235000013372 meat Nutrition 0.000 description 1
- 230000000050 nutritive effect Effects 0.000 description 1
- 229910052698 phosphorus Inorganic materials 0.000 description 1
- 239000011574 phosphorus Substances 0.000 description 1
- 239000000243 solution Substances 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K61/00—Culture of aquatic animals
- A01K61/10—Culture of aquatic animals of fish
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K61/00—Culture of aquatic animals
- A01K61/10—Culture of aquatic animals of fish
- A01K61/17—Hatching, e.g. incubators
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
- Y02A40/80—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in fisheries management
- Y02A40/81—Aquaculture, e.g. of fish
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Environmental Sciences (AREA)
- Marine Sciences & Fisheries (AREA)
- Zoology (AREA)
- Animal Husbandry (AREA)
- Biodiversity & Conservation Biology (AREA)
- Agricultural Chemicals And Associated Chemicals (AREA)
- Farming Of Fish And Shellfish (AREA)
Abstract
The invention discloses a kind of loach and Misgurnus auguillicaudatus cross breeding method is related to, belong to technical field of aquaculture.The method of the present invention is the following steps are included: parent culture, pond management, artificial insemination and hatching, seed rearing.Loach fry survival rate can be improved in method provided by the present invention, and platform ♂ × blueness ♀ first-filial generation rate of fertilization, hatching rate and survival rate height, the speed of growth cultivated using the method for the present invention are fast, and form body colour and mouthfeel are close to loach.
Description
Technical field
The invention belongs to technical field of aquaculture, specifically, the present invention relates to a kind of loach and Misgurnus auguillicaudatus are miscellaneous
Mating system is handed over, loach fry survival rate can be improved in the method for the present invention.
Background technique
Loach is a kind of small-sized, special economic fish, small, and thorn is few, and eating mouth feel is good.Loach nutritive and medicinal value is equal
It is higher, the laudatory titles such as " ginseng in water ", " soft gold " are enjoyed, health preserving dietotherapy function is gradually approved by masses.Loach is due to producing
The difference on ground, living environment, quality also have certain difference.China original inhabitants loach kind mainly has loach and big phosphorus pair mud
Loach, the former has the comparatively ideal bodily form and body colour, and good in taste, the very fast premunition of the latter's speed of growth is strong.Due to big squama pair mud
Loach (Chinese snake head) speed of growth is fast, feed coefficient is low, premunition is strong, does not like the characteristics such as sludge bar, it has also become China loach
Propagate principal item artificially, but Misgurnus auguillicaudatus shape, meat and mouthfeel are poor, at home most area market acceptance
It is low.Not only there is diploid loach in China, also widely distributed to have the biggish Tetraploid Loach, Misgurnus of individual.Therefore, two kinds of loach are utilized
Hybridization, is expected to the cross combination of discovery advantage.In addition, loach fry cultivation at present can occur often in actual production operation
Lower phenomenon, especially spray are survived to the survival rate between cun seedling generally between 20~30%, increases seed cost.This
The major reason that stage causes seed survival rate low is that pond management is not in place, and natural enemy is not removed thoroughly in water body.
In conclusion problem of the existing technology is:
Loach fry breeding phase management method is improper, and survival rate is low, increases aquaculture cost, while Misgurnus auguillicaudatus shape
State and mouthfeel are bad, are not able to satisfy most domestic area consumer demand.
The above-mentioned reason in basis, spy propose the application.
Summary of the invention
In view of the problems of the existing technology, the purpose of the present invention is to provide a kind of loach hybridize with Misgurnus auguillicaudatus it is numerous
Method is educated, loach fry survival rate can be improved in the method for the present invention.
In order to realize above-mentioned purpose of the invention, The technical solution adopted by the invention is as follows:
A kind of loach and Misgurnus auguillicaudatus cross breeding method, described method includes following steps:
(1) parent culture
(1) parent pond prepares
Traffic convenience is selected, is had abundant water resources, environment quiet, water quality is good, soil neutral or subacidity, loess or loam
Loach and Misgurnus auguillicaudatus parent pond are built in place, and 2~3 mu of area, pond 1.0~1.5m of depth, water level keeps 0.6~
Drainage system is arranged in 1.0m, bottom of pond portion mud 15~25cm of depth, and water inlet is higher by 20~40cm of the water surface, and drainage mouth is all provided with
Set anti-escape strainer；
(2) it cleans up the pond
Before putting parent in a suitable place to breed, using quick lime band water sterilization, after 5d, new water is infused to 0.6~1.0m of the depth of water；
(3) it launches
Loach parent's specification requirement: Yichuan loach, female loach body is long > 15cm, weight > 30g, and the male long body of loach body is long >
13cm, weight > 24g；Misgurnus auguillicaudatus, female loach body is long > 20cm, weight > 50g, and male loach body is long > 16cm, weight > 30g；Parent is put
Supporting density is male and female each 2500 and 5000 tails/mu, and loach and the Misgurnus auguillicaudatus point pool cultivate；
(2) pond manages
(1) parent Chi Guanli
Parent, which carries out in pond, patrols pool work, and observation loach situation of ingesting pays attention to according to weather, water quality, lower water temperature daily ration, feeding quantity
Anti-escape leakproof anti-vulnerability；In disease suffering season, drug disinfection desinsection is dissipated with povidone iodine, expelling parasite weekly；
(2) nursery pond management
Disinfection work is carried out before launching seed, specific as follows:
10d before launching infuses new water after 5d with quick lime band water sterilization；3d before launching, full pool spilling head imidacloprid desinsection；It throws
Preceding 3d, full pool spilling head compound amino acid and fermentation farmyard manure rich water are put, planktonic organism bait is cultivated；
After launching seed 4d, full pool spilling head imidacloprid desinsection 1 time；
After launching seed 14d, full pool spilling head imidacloprid desinsection 1 time；
Hereafter, every 20d, full pool spilling head imidacloprid desinsection 1 time；
Wherein: the dosage of the imidacloprid is every time 0.2~0.6kg/ mu, the dosage of the compound amino acid for 4~
6kg/ mus, the dosage of the fermentation farmyard manure is 80~120kg/ mus；
(3) artificial insemination and hatching
(1) early April, water temperature rise to 22~28 DEG C, catch loach and Misgurnus auguillicaudatus parent using cage, select one by one
The close loach to reach maturity develops bad close loach go back to pond and continues to cultivate, and it is 4:1 that male and female, which select ratio, entire election process control
Within 2h；
(2) close loach injects the dedicated compound salmon gonadotropin releasing hormone analogues of loach, and female loach injection volume is
0.7mL/ kilograms, male loach dosage halves, and using muscle of back injecting method, needle point and muscle of back are in 45 degree of angles, to head side
To injection of medicine, then 0.15~0.25cm of injection depth is temporarily supported the close loach for having injected oxytocins respectively in hanging with fish nest
In hatchery；
(3) it after 12h, carries out ovum artificial collection fertilization: female loach abdomen gently being pressed to squeeze into ovum in dry vessel, ovum is placed
Time should not too long, and ovum weight will inseminate as early as possible after reaching 0.8 kilogram, under < 25 DEG C of low temperature environment, by male loach
Sperm is squeezed into physiological saline, is poured into ovum after being gently mixed uniformly with feather, being gently mixed with feather fills smart ovum
Divide mixing, pollution-free yellow mud water stirring is then added and carries out unsticking processing, fertilized eggs go to hatching barrel hatching after unsticking；
(4) after fertilized eggs are transferred to hatching barrel, start increased water flow, allow fertilized eggs constantly to roll upwards with water flow, water temperature
22~28 DEG C, loach seedlings are hatched after 26~30h, are transferred to storage pond, holding pond, and after 2d, loach water flower can be transferred to ready nursery pond
In；
(5) cross combination are as follows: platform ♂ × blueness ♀, platform ♀ × blueness ♂, while platform ♂ × platform ♀, blueness ♂ × blueness ♀ inbred combinations conduct
Control, in which: platform: Chinese snake head, Misgurnus auguillicaudatus；It is green: green loach, Yichuan loach；
(4) seed rearing
Packed good loach seedlings are oxygenated using plastics, after transporting to nursery pond, dress seedling bag are floated in water, after 30min, into bag
Water is filled, loach seedlings is allowed to swim out of automatically, spray throw-in density is 600,000 tails/mu；
Put the seedling time: to be advisable when the morning 9~10；
It puts 1~7d after seedling, every morning, noon and feeds appropriate soya-bean milk, milk powder and yolk at dusk；
After putting seedling 8d, every morning, noon and dusk feed soya-bean milk, milk powder and the dedicated powder of loach, and soya-bean milk and milk powder are thrown
It feeds amount to reduce by day, powder increases by day, and powder dosage is no more than 4.0kg/ mus daily；
After putting seedling 16d, every morning, noon and feed the dedicated powder of loach at dusk, feeding volume by 4.0kg/ mus gradually on
It adjusts；
After putting seedling 25d, every morning, noon and dusk feed No. 0 loach special feed, first impregnate 3min before feeding, so
Full pond is uniformly thrown and is spread afterwards, feeds thereafter according to loach situation of ingesting；
It is above with reference to feeding volume in the case of weather, proper temperature, actually feeding situation will be according to specific weather, temperature
It is fed with loach situation of ingesting.
Further, (four) seed rearing described in above-mentioned technical proposal, put No. 0 loach fed after seedling 25d in step
The dosage of special feed is 6.0~10.0kg/ mus, preferably 8.0kg/ mus.
Further, the quick lime in (2) step, step of cleaning up the pond of (one) parent culture described in above-mentioned technical proposal is used
Preferably 100 pounds/acre of amount.
Further, (2) step of the management of (two) pond described in above-mentioned technical proposal, the life in nursery pond management process
Lime consumption is preferably 100 pounds/acre.
Further, (2) step of the management of (two) pond described in above-mentioned technical proposal, the pyrrole in nursery pond management process
Worm quinoline dosage is preferably 0.4kg/ mus, and the dosage of the compound amino acid is 5kg/ mus, and the dosage of the fermentation farmyard manure is
100kg/ mus.
Further, (4) step of (three) artificial insemination described in above-mentioned technical proposal and hatching, incubating oosperm step
In the incubating oosperm time be preferably 28h.
Further, (four) seed rearing described in above-mentioned technical proposal, put 1~7d after seedling and feed soya-bean milk tool in step
Body is fed after soya bean to be broken into soya-bean milk, and the dosage of the soya bean is 0.4~0.6kg/ mus, preferably 0.5kg/ mus, milk powder dosage
Be 0.3~0.5kg/ mus, preferably 0.4kg/ mus, yolk dosage is 8~12/mu, preferably 10/mu, wherein the milk powder and
Yolk be all made of water dispersion it is uniform after fed.
Further, (four) seed rearing described in above-mentioned technical proposal, to put the soya-bean milk that seedling 8d is fed in step specific
It is to be fed after soya bean to be broken into soya-bean milk, the dosage of the soya bean is 0.4~0.6kg/ mus, and preferably 0.5kg/ mus, milk powder dosage is
0.3~0.5kg/ mus, preferably 0.4kg/ mus, the dedicated powder dosage of loach is 0.4~0.6kg/ mus, preferably 0.5kg/ mus.
Compared with prior art, the invention has the following beneficial effects:
First filial generation seedling fertilized eggs, hatching rate and the survival rate cultivated using the method for the present invention are higher, and survival rate reaches
68%, and the speed of growth is fast, in addition, platform ♂ of the invention × first filial generation blueness ♀ form, body colour and mouthfeel are close to loach.
Specific embodiment
Below with reference to case study on implementation, invention is further described in detail.The implementation case before being with the technology of the present invention
It puts and is implemented, provide detailed embodiment and specific operating process now to illustrate that the present invention is creative, but this
The protection scope of invention case study on implementation not limited to the following.
The information for including according to the application, to those skilled in the art can be easily to essence of the invention
Really description carries out various changes, without departing from spirit and scope of the appended claims.It should be understood that the scope of the present invention is not
Process, property defined by being confined to or component, because these embodiments and other descriptions are just for the sake of schematic
Illustrate certain aspects of the present disclosure.In fact, this field or those skilled in the relevant art obviously can be to embodiment party of the present invention
The various changes that formula is made all cover within the scope of the appended claims.
It is not intended to limit the scope of the invention for a better understanding of the present invention, expression dosage used in this application,
All numbers of percentage and other numerical value, are understood to be modified with word " about " in all cases.Therefore,
Unless stated otherwise, otherwise digital parameters listed in specification and appended book are all approximations, may
It can be changed according to the difference for the desirable properties for attempting to obtain.Each digital parameters at least should be considered as according to being reported
Effective digital and obtained by the conventional method of rounding up.
Embodiment 1
A kind of loach of the present embodiment and Misgurnus auguillicaudatus cross breeding method, described method includes following steps:
(1) parent pond prepares
Traffic convenience is selected, is had abundant water resources, environment quiet, water quality is good, soil neutral or subacidity, loess or loam
Loach and Misgurnus auguillicaudatus parent pond are built in place, and 2~3 mu of area, pond 1.0~1.5m of depth, water level keeps 0.8m or so,
Drainage system is arranged in bottom of pond portion mud depth 20cm or so, and water inlet is higher by water surface 30cm or so, and drainage mouth is respectively provided with anti-
Escape strainer.
(2) it cleans up the pond
It before putting parent in a suitable place to breed, is sterilized with quick lime band water (15cm or so), after dosage is 100 pounds/acre, 5 days, infuses new water extremely
Depth of water 0.8m or so.
(3) it launches
Loach parent's specification requirement: Yichuan loach, female loach body is long > 15cm, weight > 30g, and the male long body of loach body is long >
13cm, weight > 24g；Loach parent's specification requirement: Chinese snake head, female loach body is long > 20cm, weight > 50g, and the male long body of loach body is long >
16cm, weight > 30g.Parent's breeding density is male and female each 2500 and 5000 tails/mu, and loach and Chinese snake head divide half congealed cultivation.
(2) pond manages
(1) parent Chi Guanli.
Parent, which carries out in pond, patrols pool work, and observation loach situation of ingesting pays attention to according to weather, water quality, lower water temperature daily ration, feeding quantity
Anti-escape leakproof anti-vulnerability.In disease suffering season, drug disinfection desinsection is dissipated with povidone iodine, expelling parasite weekly.
(2) nursery pond management
Disinfection work is carried out before launching seed, specific as follows:
10d before launching is sterilized with quick lime band water (15cm or so), and dosage is 100 pounds/acre, and new water is infused after 5d；Before dispensing
3d, full pool spilling head imidacloprid (0.4 kg/acre) desinsection.3d before launching, full pool spilling head compound amino acid (5kg/ mus) and fermentation agriculture
Fertile (100kg/ mus) rich water of family, cultivates planktonic organism bait.
After launching seed 4d, full pool spilling head imidacloprid (0.4 kg/acre) desinsection is primary.
After launching seed 14d, full pool spilling head imidacloprid (0.4 kg/acre) desinsection is primary.
Hereafter, every 20d, full pool spilling head imidacloprid (0.4 kg/acre) desinsection 1 time.
(3) artificial insemination and hatching
(1) early April, water temperature rise to 25 DEG C or so, catch loach and Chinese snake head parent using cage.Hair is selected one by one
It is bred as ripe close loach, bad close loach go back to pond is developed and continues to cultivate, it is 4:1 that male and female, which select ratio, and entire election process control exists
Within 2h.
(2) close loach injection compound salmon gonadotropin releasing hormone analogues (loach is dedicated), female loach injection volume are
0.7mL/ kilograms, male loach dosage halves.Using muscle of back injecting method, needle point and muscle of back are in 45 degree of angles, to head side
To injection of medicine, injection depth 0.2cm or so.The close loach for having injected oxytocins is temporarily supported respectively in the hatchery for hanging with fish nest
In.
(3) after 12h, ovum artificial collection fertilization is carried out.Female loach abdomen is gently pressed to squeeze into ovum in dry vessel, ovum is placed
Time should not too long, and ovum weight will inseminate as early as possible after reaching 0.8 kilogram.(< 25 DEG C) at low ambient temperatures, by male loach
Sperm is squeezed into 40mL physiological saline, is poured into ovum after being gently mixed uniformly with feather, being gently mixed with feather makes essence
Ovum is sufficiently mixed, and the pollution-free yellow mud water stirring of 50mL is then added and carries out unsticking processing, fertilized eggs go to hatching barrel and incubate after unsticking
Change.
(4) after fertilized eggs are transferred to hatching barrel, start increased water flow, allow fertilized eggs constantly to roll upwards with water flow, water temperature
25 DEG C or so, loach seedlings are hatched after 28h, are transferred to storage pond, holding pond, after 2d, loach water flower can be transferred in ready nursery pond.
(5) cross combination is platform ♂ × blueness ♀, platform ♀ × blueness ♂, while platform ♂ × platform ♀, blueness ♂ × blueness ♀ inbred combinations conduct
Compare (platform: Chinese snake head, Misgurnus auguillicaudatus；It is green: green loach, Yichuan loach).Platform ♂ × blueness ♀, platform ♂ × platform ♀, green ♂ × blueness
The rate of fertilization that ♀ combines fertilized eggs is higher, and up to 90% or so, and platform ♀ × blueness ♂ combination fertilized eggs rate of fertilization only has 58% left side
It is right；The hatching rate of platform ♂ × blueness ♀, platform ♂ × platform ♀, blueness ♂ × blueness ♀ combination fertilized eggs is higher, up to 80% or so, and platform ♀ × blueness ♂
The rate of fertilization of combination fertilized eggs only has 50% or so.Table 1 specific as follows.
1 various combination artificial propagation rate of fertilization of table, hatching rate contrast table
(4) seed rearing
Packed good loach seedlings are oxygenated using plastics, after transporting to nursery pond, dress seedling bag are floated in water, after 30min, into bag
Water is filled, loach seedlings is allowed to swim out of automatically.Spray throw-in density is 600,000 tails/mu.
Put the seedling time: to be advisable when the morning 9~10.
It puts 1~7d after seedling, every morning, noon and feeds soya-bean milk (0.5kg soya bean/mu), milk powder (0.4kg/ mus) at dusk
With yolk (10/mu, opened with aquation).
Put after seedling after 8d, every morning, noon and feed at dusk soya-bean milk (0.5kg soya bean/mu), milk powder (0.4kg/ mus) and
The dedicated powder of loach (0.5kg/ mus), soya-bean milk and milk powder feeding volume are reduced by day, and powder increases by day.
After putting seedling 16d, every morning, noon and feed the dedicated powder of loach at dusk, feeding volume by 4.0kg/ mus gradually on
It adjusts.
After putting seedling 25d, every morning, noon and dusk feed No. 0 loach special feed (8.0kg/ mus), first soak before feeding
3min is steeped, then full pond is uniformly thrown and spread, and feeds thereafter according to loach situation of ingesting.
It is above with reference to feeding volume in the case of weather, proper temperature, actually feeding situation will be according to specific weather, temperature
It is fed with loach situation of ingesting.
By culture in 4 months, various combination first filial generation seed survival rate, weight, form and feeding habits, discovery are recorded
4 kinds of combination seed survival rates all reach 60% or more, weight size be successively platform ♂ × platform ♀ > platform ♀ × blueness ♂ > platform ♂ × blueness ♀ >
Green ♂ × blueness ♀, form body colour and feeding habits are all similar to female parent, wherein the platform ♂ × first filial generation blueness ♀ form body colour and mouthfeel with
Green loach is relatively.Table 2 specific as follows.
2 various combination artificial propagation first filial generation survival rate of table, weight, form and feeding habits
The above results show that loach fry survival rate not only can be improved in this method, form, body colour and mouth are also cultivated
Sense is all close to the platform ♂ of loach × blueness ♀ first-filial generation.
Claims (5)
1. a kind of loach and Misgurnus auguillicaudatus cross breeding method, it is characterised in that: described method includes following steps:
(1) parent culture
(1) parent pond prepares
Traffic convenience is selected, is had abundant water resources, environment quiet, water quality is good, soil neutral or subacidity, the place of loess or loam
Loach and Misgurnus auguillicaudatus parent pond are built, 2~3 mu of area, pond 1.0~1.5m of depth, water level keeps 0.6~1.0m, pond
Drainage system is arranged in bottom sludge 15~25cm of depth, and water inlet is higher by 20~40cm of the water surface, and drainage mouth is respectively provided with anti-escape
Strainer；
(2) it cleans up the pond
Before putting parent in a suitable place to breed, using quick lime band water sterilization, after 5d, new water is infused to 0.6~1.0m of the depth of water；
(3) it launches
Loach parent's specification requirement: Yichuan loach, female loach body is long > 15cm, weight > 30g, and the male long body of loach body is long > 13cm, body
Weight > 24g；Misgurnus auguillicaudatus, female loach body is long > 20cm, weight > 50g, and male loach body is long > 16cm, weight > 30g；Parent's breeding density
For male and female each 2500 and 5000 tails/mu, loach and the Misgurnus auguillicaudatus point pool are cultivated；
(2) pond manages
(1) parent Chi Guanli
Parent, which carries out in pond, patrols pool work, and observation loach situation of ingesting pays attention to anti-escape according to weather, water quality, lower water temperature daily ration, feeding quantity
Leakproof anti-vulnerability；In disease suffering season, drug disinfection desinsection is dissipated with povidone iodine, expelling parasite weekly；
(2) nursery pond management
Disinfection work is carried out before launching seed, specific as follows:
10d before launching infuses new water after 5d with quick lime band water sterilization；3d before launching, full pool spilling head imidacloprid desinsection；Before dispensing
3d, full pool spilling head compound amino acid and fermentation farmyard manure rich water, cultivate planktonic organism bait；
After launching seed 4d, full pool spilling head imidacloprid desinsection 1 time；
After launching seed 14d, full pool spilling head imidacloprid desinsection 1 time；
Hereafter, every 20d, full pool spilling head imidacloprid desinsection 1 time；
Wherein: the dosage of the imidacloprid is 0.2~0.6kg/ mus every time, and the dosage of the compound amino acid is 4~6kg/ mus,
The dosage of the fermentation farmyard manure is 80~120kg/ mus；
(3) artificial insemination and hatching
(1) early April, water temperature rise to 22~28 DEG C, catch loach and Misgurnus auguillicaudatus parent using cage, select development one by one
Mature close loach develops bad close loach go back to pond and continues to cultivate, and it is 4:1 that male and female, which select ratio, and entire election process control is in 2h
Within；
(2) close loach injects the dedicated compound salmon gonadotropin releasing hormone analogues of loach, and female loach injection volume is 0.7mL/ public
Jin, male loach dosage halve, and using muscle of back injecting method, needle point and muscle of back are in 45 degree of angles, inject medicine to cephalad direction
Then object, 0.15~0.25cm of injection depth temporarily support the close loach for having injected oxytocins in the hatchery for hanging with fish nest respectively；
(3) it after 12h, carries out ovum artificial collection fertilization: gently pressing female loach abdomen to squeeze into ovum in dry vessel, ovum standing time
Should not too long, ovum weight will inseminate as early as possible after reaching 0.8 kilogram, under < 25 DEG C of low temperature environment, by the sperm of male loach
It is squeezed into physiological saline, is poured into ovum after being gently mixed uniformly with feather, being gently mixed with feather keeps smart ovum sufficiently mixed
It closes, pollution-free yellow mud water stirring is then added and carries out unsticking processing, fertilized eggs go to hatching barrel hatching after unsticking；
(4) after fertilized eggs are transferred to hatching barrel, start increased water flow, fertilized eggs is allowed constantly to roll upwards with water flow, water temperature 22~
28 DEG C, loach seedlings are hatched after 26~30h, are transferred to storage pond, holding pond, after 2d, loach water flower can be transferred in ready nursery pond；
(5) cross combination are as follows: platform ♂ × blueness ♀, platform ♀ × blueness ♂, while platform ♂ × platform ♀, blueness ♂ × blueness ♀ inbred combinations conduct pair
According to, in which: platform: Chinese snake head, Misgurnus auguillicaudatus；It is green: green loach, Yichuan loach；
(4) seed rearing
Packed good loach seedlings are oxygenated using plastics, after transporting to nursery pond, dress seedling bag is floated in water, after 30min, pond is filled into bag
Water allows loach seedlings to swim out of automatically, and spray throw-in density is 600,000 tails/mu；
Put the seedling time: to be advisable when the morning 9~10；
It puts 1~7d after seedling, every morning, noon and feeds appropriate soya-bean milk, milk powder and yolk at dusk；
After putting seedling 8d, every morning, noon and dusk feed soya-bean milk, milk powder and the dedicated powder of loach, soya-bean milk and milk powder feeding volume
It is reduced by day, powder increases by day, and powder dosage is no more than 4.0kg/ mus daily；
After putting seedling 16d, every morning, noon and dusk feed the dedicated powder of loach, and feeding volume is gradually raised by 4.0kg/ mus；
After putting seedling 25d, every morning, noon and dusk feed No. 0 loach special feed, first impregnate 3min before feeding, then entirely
Pond is uniformly thrown and is spread, and feeds thereafter according to loach situation of ingesting.
2. loach according to claim 1 and Misgurnus auguillicaudatus cross breeding method, it is characterised in that: described (two) pond
(2) step of pool management, the imidacloprid dosage in nursery pond management process are 0.4kg/ mus, and the dosage of the compound amino acid is
5kg/ mus, the dosage of the fermentation farmyard manure is 100kg/ mus.
3. loach according to claim 1 and Misgurnus auguillicaudatus cross breeding method, it is characterised in that: (three) people
The incubating oosperm time in (4) step of work insemination and hatching, incubating oosperm step is 28h.
4. loach according to claim 1 and Misgurnus auguillicaudatus cross breeding method, it is characterised in that: described (four) seedling
It is specifically to feed after soya bean is broken into soya-bean milk that 1~7d, which feeds the soya-bean milk in step, after planting cultivation, putting seedling, and the dosage of the soya bean is
0.4~0.6kg/ mus, milk powder dosage is 0.3~0.5kg/ mus, and yolk dosage is 8~12/mu, wherein the milk powder and egg
Huang be all made of water dispersion it is uniform after fed.
5. loach according to claim 1 and Misgurnus auguillicaudatus cross breeding method, it is characterised in that: described (four) seedling
It kind cultivates, put that feed the dedicated powder dosage of loach in step after seedling 8d be 0.4~0.6kg/ mus.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201811472997.5A CN109349172B (en) | 2018-12-04 | 2018-12-04 | Hybrid breeding method for loaches and paramisgurnus dabryanus |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201811472997.5A CN109349172B (en) | 2018-12-04 | 2018-12-04 | Hybrid breeding method for loaches and paramisgurnus dabryanus |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN109349172A true CN109349172A (en) | 2019-02-19 |
| CN109349172B CN109349172B (en) | 2020-12-11 |
Family
ID=65331258
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201811472997.5A Active CN109349172B (en) | 2018-12-04 | 2018-12-04 | Hybrid breeding method for loaches and paramisgurnus dabryanus |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN109349172B (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110663593A (en) * | 2019-10-28 | 2020-01-10 | 江西农业大学 | Construction method of hybrid loach with fast growth and good meat quality and suitable for paddy field cultivation |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPH08131014A (en) * | 1994-11-11 | 1996-05-28 | Masao Narita | Preparation of fertilized egg of loach |
| CN103300046A (en) * | 2012-03-15 | 2013-09-18 | 浙江森得保生物制品有限公司 | Preparation method of imidacloprid liquid and control on longicorn larva |
| CN107494343A (en) * | 2017-07-31 | 2017-12-22 | 兰溪市普润斯水产养殖技术有限公司 | A kind of artificial fecundation method of loach |
-
2018
- 2018-12-04 CN CN201811472997.5A patent/CN109349172B/en active Active
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPH08131014A (en) * | 1994-11-11 | 1996-05-28 | Masao Narita | Preparation of fertilized egg of loach |
| CN103300046A (en) * | 2012-03-15 | 2013-09-18 | 浙江森得保生物制品有限公司 | Preparation method of imidacloprid liquid and control on longicorn larva |
| CN107494343A (en) * | 2017-07-31 | 2017-12-22 | 兰溪市普润斯水产养殖技术有限公司 | A kind of artificial fecundation method of loach |
Non-Patent Citations (3)
| Title |
|---|
| 徐在宽等: "《泥鳅高效养殖100例》", 31 May 2015, 第256-284页 * |
| 祖国掌等: "《科学养鱼大全》", 31 March 2015, 安徽科学技术出版社 * |
| 龙良启等: "《泥鳅与大鳞副泥鳅正反交子代遗传变异的RAPD分析》", 《水生生物学报》 * |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110663593A (en) * | 2019-10-28 | 2020-01-10 | 江西农业大学 | Construction method of hybrid loach with fast growth and good meat quality and suitable for paddy field cultivation |
Also Published As
| Publication number | Publication date |
|---|---|
| CN109349172B (en) | 2020-12-11 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN103875571B (en) | The method of cultivation of a kind of mandarin fish fingerling mass-producing | |
| CN105994027B (en) | A kind of cultural method and pond for improving pond Procambius clarkii commercial specification and yield | |
| CN100423633C (en) | A high-yield method for breeding pelteobagrus fulvidraco in pond | |
| CN105746411B (en) | A kind of foster method of bottom of pond kind blueness cray fingerling company | |
| CN105393985A (en) | Frog culturing method | |
| CN103718991A (en) | Ecological breeding method of paddy field polyculture of pseudorasbora parva and procambarus clarkii | |
| CN112042570B (en) | Method for efficiently cultivating and domesticating micropterus salmoides water bloom seedlings in pond greenhouse | |
| CN106561517A (en) | Crayfish propagating and breeding method | |
| CN103563800A (en) | Net cage three-dimensional breeding method for ricefield eel and loach | |
| CN101707986B (en) | Pelteobagrus vachelli and pseudobagrus ussuriensis cross breeding method | |
| CN101836607A (en) | Method for breeding macrobrachium nipponensis | |
| CN106818438A (en) | A kind of method of the floating bed cage culture swamp eel using vegetables of sheltering from heat or light | |
| CN107494097A (en) | Rice field stereo synthesis breeding method | |
| CN107173277A (en) | The acclimation method and cultural method of a kind of sturgeon | |
| CN109089799A (en) | A kind of cultural method that rice shrimp educates altogether | |
| CN110122385A (en) | A kind of mixing zoology breeding method of high-quality rice shrimp crab | |
| CN105210983A (en) | Breeding and cultural method of rocky mountainous area wild boar and Guangxi Huanjiang Xiang pig Hybrid | |
| CN106561568A (en) | Yellow-colored cattle ecological culturing method | |
| CN109042416A (en) | A kind of early breeding method of Procambius clarkii | |
| CN103493767A (en) | Cross breeding method of odontobutis potamophila and odontobutis yaluensis | |
| CN102715115A (en) | Phoxinus lagowskii fry breeding method | |
| CN107372301A (en) | A kind of cultural method for promoting soft-shelled turtle growth | |
| CN106719393A (en) | A kind of outdoor cultural method of wild boar | |
| KR20140040336A (en) | Aquaculture method for a cynoglossus semilaevis | |
| CN105941230A (en) | Grass carp reproduction and breeding technology |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| GR01 | Patent grant | ||
| GR01 | Patent grant |