CN109321489A - A kind of rice shrimp mixed breeding field complex microbial inoculum - Google Patents

A kind of rice shrimp mixed breeding field complex microbial inoculum Download PDF

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Publication number
CN109321489A
CN109321489A CN201811141482.7A CN201811141482A CN109321489A CN 109321489 A CN109321489 A CN 109321489A CN 201811141482 A CN201811141482 A CN 201811141482A CN 109321489 A CN109321489 A CN 109321489A
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China
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mass parts
bacillus
bacteria
microbial inoculum
complex microbial
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CN201811141482.7A
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冀洪策
刘泽新
王颖
崔志慧
孙立黎
高敬伟
赵海萍
赵金锁
杨晋
吕晓丽
冀洪权
江硕
张颖
黄河清
刘洋
郝迎春
冀潜
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Dengzhou Huihai Agricultural Technology Co Ltd
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Dengzhou Huihai Agricultural Technology Co Ltd
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Priority to CN201811141482.7A priority Critical patent/CN109321489A/en
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    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/20Bacteria; Culture media therefor

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  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Biotechnology (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Medicinal Chemistry (AREA)
  • Microbiology (AREA)
  • Biomedical Technology (AREA)
  • Virology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Agricultural Chemicals And Associated Chemicals (AREA)

Abstract

A kind of rice shrimp mixed breeding field complex microbial inoculum, includes photosynthetic bacteria, bacillus subtilis, zinc sulfate, four kinds of strains of bacillus licheniformis and the beet sucrose as additive;The above strain and beet sucrose are pressed mass parts: 18 mass parts of photosynthetic bacteria, 12 mass parts of bacillus subtilis, 20 mass parts of beet sucrose, 2 mass parts of zinc sulfate, 5 mass parts of bacillus licheniformis are mixed and made into intermixture;The complex microbial inoculum further includes having 43 mass parts of pure water;The pure water is mixed to get composite bacteria agent as diluent and intermixture;Wherein, photosynthetic bacteria, bacillus subtilis, bacillus licheniformis in composite bacteria agent living bacteria count amount >=200,000,000/liter;Photosynthetic bacteria used can be Rhodopseudomonas palustris;Bacillus licheniformis used can be side bacillus, bacillus polymyxa;The photosynthetic bacteria can be Rhodospirillum rubrum, purple nonsulfur bacteria.

Description

A kind of rice shrimp mixed breeding field complex microbial inoculum
Technical field
The invention belongs to rice shrimp fields to administer microbial inoculum technical field, and in particular to a kind of rice shrimp mixed breeding field microbial composite bacteria Agent.
Background technique
Cray, also referred to as Procambius clarkii, red crayfish and freshwater crayfish;Be similar to shrimp and crust is hard, adult is about 5.6 ~ 11.9 centimetres, kermesinus, the nearly black in crust part, belly back side has a wedge-shaped striped;Juvenile prawn body is uniform grey, sometimes Has black ripple;Chela is long and narrow, is not separated by areola in the middle part of crust, obviously has particle on crust;Volume sword has gemmule or volume Sword end has indentation;It is economic freshwater shrimps, because meat flavour is delicious wide welcomed by the people;Because of its omnivorousness, the speed of growth is fast, adapts to Ability is strong and absolute competitive advantage is formed in local environment;Its range of ingesting include water plant, algae, aquatic insect, Animal carcass etc., it is a kind of new cray aquaculture model that shrimp is supported in rice field, is realized by the feeding shrimp of rice field kind rice furrow maximum Economic benefit;But rice field supports shrimp and still there is certain risk, mismanagement will lead to the cray underproduction even death, to cultivation Bring great challenge in family;Scientific and reasonable cultivation is that cultivation is successfully crucial;
Cray survival ability is strong, can survive in harsh environment, but this does not represent cray and likes such life Environment, often there are this cognition mistaken ideas in people, so the living environment of cray has been rectified and improved, for the growth and breeding of cray It is all crucial point;In existing rice shrimp mixed breeding field Treatment process now, rice shrimp raise together field still exist with Lower problem: first is that the stalk of rice will affect geological environment after rotting, geology is caused to deteriorate;Second is that existing compound on the market The single effect that microbial inoculum generates, and the effect is unsatisfactory;As long-term rice shrimp is raised together, the effect of generation can become increasingly It is small;It causes water body to deteriorate repeatedly, generates the various toxin for endangering aquatic biological, increase harmful bacteria in water body, beneficial bacterium subtracts It is few, keep microbe composition unbalance, is unfavorable for rice, shrimp growth.
Summary of the invention
The present invention provides a kind of complex microbial inoculum for rice shrimp mixed breeding field, and production is simple, and cost performance is high, and effect Significantly;Intervened for rice shrimp water in field matter and harmful components, handled, optimized, improves the environment of rice shrimp mixed breeding.
The technical solution of the present invention is as follows: a kind of rice shrimp raises together field complex microbial inoculum, it include photosynthetic bacteria, withered grass bud Spore bacillus, zinc sulfate, four kinds of strains of bacillus licheniformis and the beet sucrose as additive;
The above strain and beet sucrose are pressed into mass parts: 18 mass parts of photosynthetic bacteria, 12 mass parts of bacillus subtilis, beet sugarcane Sugared 20 mass parts, 2 mass parts of zinc sulfate, 5 mass parts of bacillus licheniformis are mixed and made into intermixture;The microorganism is compound Microbial inoculum further includes having 43 mass parts of pure water;The pure water is mixed to get composite bacteria agent as diluent and intermixture;Its In, photosynthetic bacteria, bacillus subtilis, bacillus licheniformis in composite bacteria agent living bacteria count amount >=200,000,000/liter.
Photosynthetic bacteria used can be Rhodopseudomonas palustris.
Bacillus licheniformis used can be side bacillus, bacillus polymyxa.
The photosynthetic bacteria can be Rhodospirillum rubrum, purple nonsulfur bacteria.
Beneficial effect caused by the technical program is:
Provided by the present invention for the complex microbial inoculum raw material environmental protection in rice shrimp field, cost performance is high;Exist for rice shrimp Tanaka Outstanding problem tool improve significantly governance role;This composite bacteria agent includes photosynthetic bacteria, bacillus subtilis, beet sugarcane Sugar, zinc sulfate, bacillus licheniformis;
Wherein bacillus licheniformis, bacillus subtilis the meeting extracellular proteinase, cellulase, amylase after cultivation and fermentation, It improves significantly to polluted rice shrimp water in field matter, declines turbidity;Inhibition is generated to harmful bacteria, pathogenic bacteria in water quality Effect;Further, photosynthetic bacteria utilizes luminous energy, can absorb CO2, ammoniacal nitrogen, hydrogen sulfide makes organic matter mass propagation, organic Under conditions of object, it can use organic matter again and expand numerous, provide a certain amount of oxygen for water body;Purified water can also be played Matter, the effect for increasing dissolved oxygen, improving cultivation density;Research shows that: the thallus of photosynthetic bacteria is nontoxic, full of nutrition, and protein contains Amount is up to 64.1%-66%, lipase 37 .18%, crude fibre 2.78%, carbohydrate 23.0%, ash content 4.28%, every gram of dry mycelium phase When in 21KJ heat;Not only protein abundance, and amino acid composition it is complete, be the optimum nutrition strain of aqueous bio it One;And immune, controlling disease can be enhanced, improve shrimps survival rate;Various nutritions in the balanced feed of energy, improve feed Utilization rate;
Further, the protein of zinc sulfate generates in water zinc ion and polypide cell is combined into protein salt, makes its precipitating, uses In killing infusorian, insecticidal action is played;The algae such as green alga, the diatom in water, optimizing water quality can also quickly be killed;
This complex microbial inoculum can purify water in rice shrimp Tanaka, create, improve the high-quality water environment of suitable rice shrimp mixed breeding; By the breeding of beneficial bacterium, prevent the part pest and disease damage of rice root;Inhibit the survival of harmful bacteria, pathogenic bacteria;Improve rice shrimp field soil Structure and trophic structure promote the symbiosis of rice shrimp, improve survival rate.
Specific embodiment
Technical scheme is described further combined with specific embodiments below.
Embodiment
A kind of rice shrimp mixed breeding field complex microbial inoculum, includes photosynthetic bacteria, bacillus subtilis, zinc sulfate, lichens Four kinds of strains of bacillus and the beet sucrose as additive;
The above strain and beet sucrose are pressed into mass parts: 18 mass parts of photosynthetic bacteria, 12 mass parts of bacillus subtilis, beet sugarcane Sugared 20 mass parts, 2 mass parts of zinc sulfate, 5 mass parts of bacillus licheniformis are mixed and made into intermixture;The microorganism is compound Microbial inoculum further includes having 43 mass parts of pure water;The pure water is mixed to get composite bacteria agent as diluent and intermixture;Its In, photosynthetic bacteria, bacillus subtilis, bacillus licheniformis in composite bacteria agent living bacteria count amount >=200,000,000/liter.
When in use:
Bacillus subtilis, bacillus licheniformis are placed in transparent vessel, and carry out illumination 3h expand it is numerous;Then will expand Bacillus subtilis, bacillus licheniformis after numerous are removed and placed in reaction kettle, photosynthetic bacteria are added, beet sucrose is stirred It mixes, isothermal reaction temperature in the kettle is controlled at 37 DEG C, speed of agitator 150r/min, interval 10min stirring is primary, stirs every time 5min is stirred 4 times, then static 1h;Zinc sulfate stirring 5min is added, intermixture is obtained, and take out and be down to room temperature, is added pure Water purification obtains composite bacteria agent;
Composite bacteria agent is sprayed in rice shrimp field, primary rear interval is sprayed and sprays again within one week once.
This complex microbial inoculum can purify water in rice shrimp Tanaka, create, improve the high-quality water of suitable rice shrimp mixed breeding Environment;By the breeding of beneficial bacterium, prevent the part pest and disease damage of rice root;Inhibit the survival of harmful bacteria, pathogenic bacteria;Improve rice shrimp field Soil texture and trophic structure promote the symbiosis of rice shrimp;Improve survival rate.

Claims (4)

1. a kind of rice shrimp raises together field complex microbial inoculum, it is characterised in that: include photosynthetic bacteria, bacillus subtilis, sulphur Sour zinc, four kinds of strains of bacillus licheniformis and the beet sucrose as additive;
The above strain and beet sucrose are pressed into mass parts: 18 mass parts of photosynthetic bacteria, 12 mass parts of bacillus subtilis, beet sugarcane Sugared 20 mass parts, 2 mass parts of zinc sulfate, 5 mass parts of bacillus licheniformis are mixed and made into intermixture;The microorganism is compound Microbial inoculum further includes having 43 mass parts of pure water;The pure water is mixed to get composite bacteria agent as diluent and intermixture;Its In, photosynthetic bacteria, bacillus subtilis, bacillus licheniformis in composite bacteria agent living bacteria count amount >=200,000,000/liter.
2. a kind of rice shrimp according to claim 1 raises together field complex microbial inoculum, it is characterised in that: used is photosynthetic thin Bacterium can be Rhodopseudomonas palustris.
3. a kind of rice shrimp according to claim 1 raises together field complex microbial inoculum, it is characterised in that: lichens bud used Spore bacillus can be side bacillus, bacillus polymyxa.
4. a kind of rice shrimp according to claim 1 raises together field complex microbial inoculum, it is characterised in that: described is photosynthetic thin Bacterium can be Rhodospirillum rubrum, purple nonsulfur bacteria.
CN201811141482.7A 2018-09-28 2018-09-28 A kind of rice shrimp mixed breeding field complex microbial inoculum Pending CN109321489A (en)

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Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN101269884A (en) * 2008-05-08 2008-09-24 储卫华 Microorganism renovation agent of water environment and preparation method thereof
WO2012009660A2 (en) * 2010-07-15 2012-01-19 The Procter & Gamble Company Detergent compositions comprising microbially produced fatty alcohols and derivatives thereof
CN105766745A (en) * 2016-04-25 2016-07-20 简阳市大众养殖有限责任公司 Method for culturing loaches in paddy field
CN108264150A (en) * 2018-01-16 2018-07-10 四川知和环保科技有限公司 A kind of complex micro organism fungicide applies the method in water body is administered

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN101269884A (en) * 2008-05-08 2008-09-24 储卫华 Microorganism renovation agent of water environment and preparation method thereof
WO2012009660A2 (en) * 2010-07-15 2012-01-19 The Procter & Gamble Company Detergent compositions comprising microbially produced fatty alcohols and derivatives thereof
CN105766745A (en) * 2016-04-25 2016-07-20 简阳市大众养殖有限责任公司 Method for culturing loaches in paddy field
CN108264150A (en) * 2018-01-16 2018-07-10 四川知和环保科技有限公司 A kind of complex micro organism fungicide applies the method in water body is administered

Non-Patent Citations (4)

* Cited by examiner, † Cited by third party
Title
何若天: "《农用益生菌生产与应用手册》", 29 February 2016, 金盾出版社 *
罗勇胜等: ""光合细菌与芽孢杆菌协同净化养殖水体的研究"", 《农业环境科学学报》 *
肖晶晶等: ""生物强化技术优势及环保菌剂研究应用现状"", 《环境科学导刊》 *
褚天铎等: "《简明施肥技术手册》", 31 January 2014, 金盾出版社 *

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