CN101082027B

CN101082027B - Preparation of holothurian-fertilizing bacterium and method for restoring sea cucumber cultivation pool environment

Info

Publication number: CN101082027B
Application number: CN2006100271333A
Authority: CN
Inventors: 邹国忠
Original assignee: Shanghai Hongbao Green Aquatic Product Science & Technology Development Co Ltd
Current assignee: Shanghai Hongbao Green Aquatic Product Science & Technology Development Co Ltd
Priority date: 2006-05-31
Filing date: 2006-05-31
Publication date: 2011-02-16
Anticipated expiration: 2026-05-31
Also published as: CN101082027A

Abstract

The sea cucumber fattening bacterial preparation is environment friendly composite live bacterial preparation developed based on the characteristics of water and bottom material in sea cucumber raising pond and by means of modern biotechnology and for improving water quality and bottom material quality. The sea cucumber fattening bacterial preparation has photosynthetic bacteria, bacillus, saccharomycetes and actinomycete as the main bacteria, and contains trace elements essential for culturing marine micro algae for raising sea cucumber. The microbes in the sea cucumber fattening bacterial preparation is in dormant state for easy storing, and after being thrown into water, they are revived and disintegrated fast. They propagate fast to form dominant species to decompose organic pollutant in water, eliminate harmful matter, promote the growth of beneficial algae, inhibit the growth of harmful algae, and improve water and bottom material quality.

Description

Preparation of fertile ginseng bacterium and the method for repairing the holothruian cultures pool environment

Technical field

Fertile ginseng bacterium is specially at holothurian cultivation pond water quality and substrate characteristics, use the compound viable bacteria rich water of the environmental type product of modern biotechnology exploitation, and have water quality, substrate improving effect concurrently, the photosynthetic bacterium that it goes out with the nature separation and Culture, genus bacillus, yeast, actinomycetes are taken bacterial classification as the leading factor, simultaneously composite have cultivate marine microalgae (diatom, green alga, chrysophyceae), the especially needed trace element of benthic diatom in the holothruian cultures process.

Background technology

Fertile ginseng bacteria microorganism all is in dormant state, stablize and easily preservation, after rendering to water body, the dormancy viable bacteria can very fast recovery and disintegration, and breed into dominant population with multiple, decompose the organic pollutant in the water body rapidly, eliminate objectionable impuritiess such as amino nitrogen, nitroso-group nitrogen, hydrogen sulfide in the water, and be translated into the nutrition source of marine unicellular algae class, promote the growth of diatom, green alga, the good marine alga of chrysophyceae, suppress the breeding of harmful algae, play the effect of cultivating and improveing water quality, simultaneously can increase water transparency and dissolved oxygen, purify substrate.After useful bacterial strain is preponderated in water body, also can pass through nutrient competition, competition for space and secretion class antibiotic substance, suppress field planting and the invasion and attack of other pathogenic bacterium directly or indirectly, also can produce immunologic active material in addition, thereby reduce sickness rate.Fertile ginseng bacterium is without any side effects to sea cucumber and other cultured fishes, shrimp, crab, shellfish, is that high-density breeding is cultivated ginseng pond active bait, guarantees that the sea cucumber individuality is big and fleshy, improves surviving rate.

Physicochemical character: pale powder.

Main component: photosynthetic bacterium, genus bacillus, yeast, actinomycetes, seawater algae trace element, nutrient salts.

Active constituent content: every gram contains probiotic bacterium 20 * 10 ⁸Individual.

Summary of the invention

The preparation method of the fertile ginseng of a kind of aquatic products probiotics bacterium, preparation as follows:

1. get PS positively charged ion gel starch and be dissolved in water, dilute 10 times, sterilize after being made into 10% solution;

2. the 1. solution after will dissolving fully adds in the cultured EM bacterium bacterium liquid by the 1-3% addition, stirred several minutes, treat to have in the bacterium liquid thalline throw out to produce, after supernatant liquor and the concentrated throw out solid-liquid separation, go to collect somatic cells after the most of moisture with the nylon filter cloth;

3. getting 2., hyperconcentration EM bacterium somatic cells stirred several minutes by in the 5-10% addition adding dry powder ooze;

4. 60 mesh sieves select the powdery yeast bacterium, and are standby;

5. 60 mesh sieves select powdery saltpetre, and are standby;

6. 60 mesh sieves select powdery sodium silicate, and are standby;

7. 60 mesh sieves select powdery b diammonium edta sodium, and are standby;

8. 60 mesh sieves select the powdery ironic citrate, and are standby;

9. 60 mesh sieves select the powdered acid sodium dihydrogen, and are standby;

10. get 3. 4. 5. 6. 7. 8. 9. in 30: 10: 30: 22: 2: 3: 3 ratios are composite to mix.

Specific implementation method

Use range: be applicable to all sea cucumber nursery ponds, keep a full stand of seedings pond and growing pond (comprise and raise together with the pond), especially be fit to culture sea cucumber and form for many years or by the reconstruction of shrimp pond that mud is more, the aging pond that substrate is relatively poor.

Usage: evenly splash with full pond, 10-20 times of Chi Shui dilution back.

Consumption:

1, improves water quality: join bacterium 100 with fertilizer for every mu and restrain, splashed once every 5-7 days.

2, improvement substrate: join bacterium 250 with fertilizer for every mu and restrain, splashed once every 5-7 days.

3, cultivate algae: join bacterium 250-500 with fertilizer for every mu and restrain full pool spilling head, splash at any time according to water quality girth of a garment situation later on, water quality is crossed thin multiplicable splashing.

4, grow seedlings: seedling goes into pond first three day, and every cubic metre of water body 5 grams of splashing after seedling goes into the pond, are looked the water quality situation, splashes once every 2-3 days, and consumption is every cubic metre of water body 2-3 gram of splashing.

Attention:

1, throws in " fertile ginseng bacterium " bans use of germicidal action in first three day, back five days pharmaceutical chemicals and antibiotic medicine;

Water temperature should reach more than 5 ℃ when 2, throwing in, and throws in and can change water after 3-5 days.

The present invention has strong operability, with low cost, effect is remarkable, to the water surrounding non-secondary pollution, sea cucumber is had no side effect, the holothruian cultures bottom environment there are advantages such as obvious repairing effect, be to comply with current social back to nature, treasure water and life, pursue green food, the development healthy aquaculture, the only way of realizing healthy consumption.

Claims