CN109315286A - The method that screening improves maize/soybean Wheat yield nitrogen efficiency maize genotype - Google Patents

The method that screening improves maize/soybean Wheat yield nitrogen efficiency maize genotype Download PDF

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CN109315286A
CN109315286A CN201811187490.5A CN201811187490A CN109315286A CN 109315286 A CN109315286 A CN 109315286A CN 201811187490 A CN201811187490 A CN 201811187490A CN 109315286 A CN109315286 A CN 109315286A
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soybean
maize
nitrogen
root
corn
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CN109315286B (en
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郭松
秦鱼生
陈庆瑞
陈琨
樊红柱
喻华
曾祥忠
上官宇先
周子军
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Soil and Fertilizer Research Institute SAAS
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    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01HNEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
    • A01H1/00Processes for modifying genotypes ; Plants characterised by associated natural traits
    • A01H1/04Processes of selection involving genotypic or phenotypic markers; Methods of using phenotypic markers for selection
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01GHORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
    • A01G22/00Cultivation of specific crops or plants not otherwise provided for
    • A01G22/20Cereals
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01GHORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
    • A01G22/00Cultivation of specific crops or plants not otherwise provided for
    • A01G22/40Fabaceae, e.g. beans or peas

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  • Life Sciences & Earth Sciences (AREA)
  • Botany (AREA)
  • Environmental Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • General Health & Medical Sciences (AREA)
  • Developmental Biology & Embryology (AREA)
  • Pretreatment Of Seeds And Plants (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

The invention discloses a kind of methods that screening improves maize/soybean Wheat yield nitrogen efficiency maize genotype, belong to agricultural plantation technology field, its technical solution is: by screening the maize genotypes kind that the deep root type for being suitble to combine with Soybean Intercropping, root secretion ability are strong, system yield is high, then by these corn varieties be divided into deep root, shallow root and Soybean Intercropping, the physiological mechanism of maize/soybean Intercropping System nitrogen efficiency variance is analyzed, improves maize/soybean Wheat yield nitrogen efficiency maize genotype to filter out.Invention has the corn variety of different root system configurations and root secretion by screening, study nonoculture system underground Coupling effects and pair between make soybean nitrogen-fixing efficiency influence, clearly it is suitable for the Root morphology physiological characteristic of the corn variety of maize/soybean Wheat yield, the whole nitrogen efficiency of Wheat yield can be improved.

Description

The method that screening improves maize/soybean Wheat yield nitrogen efficiency maize genotype
Technical field
The invention belongs to agricultural plantation technology fields, and in particular to a kind of screening Root Characteristics difference corn variety raising is beautiful The technology of rice/Soybean Intercropping system nitrogen efficiency.
Background technique
Corn and Soybean Intercropping are a kind of Typical Dispositions for representing and making between high short-stalked crop, standing grain bean crop, are a kind of adaptations The stronger nonirrigated farmland planting patterns of property.Jade/beans mode has all been distributed in all parts of the country before the founding of the state, and Southwest China in recent years A kind of new and effective how ripe nixed planting in nonirrigated farmland of area's fast development, is increasing both production and income, increases farmland ecosystem two packing spaces Property, nitrogen utilization efficiency is improved, the important measures of fertilizer application amount are reduced." wheat-jade-beans " cropping pattern is in Sichuan Province within 2013 Popularizing area breach 400,000 hectares.Between make the advantage of yield and depend entirely on the Interspecific interaction of underground part root system, standing grain The reasonable root dry mass of undergraduate course crop and crucial root secretion play an important role in strengthening legume nodulation and nitrogen fixation, are The main path that jade/beans system nitrogen efficiently utilizes.
Crop combination and variety selection appropriate are that intercropping and interplanting is successfully crucial in intercropping and interplanting.The nitrogen of different corn varieties There are significant differences for acquisition capability and root secretion ability, this may influence the fixed nitrogen effect of soybean in Intercropping System, final shadow The nitrogen for ringing entire production system efficiently utilizes.But it has no basis at present for the selection of corn variety in Wheat yield.
Summary of the invention
For overcome it is above-mentioned there are deficiency, the present inventor attempted by long-term explorations and multiple experiment with Effort is kept reforming and is innovated, and proposes a kind of method that screening Root Characteristics difference improves maize/soybean Wheat yield nitrogen efficiency.
Its specific technical solution is: a kind of screening improves the side of maize/soybean Wheat yield nitrogen efficiency maize genotype Method, it is characterised in that: including following operation:
A, screening is suitble to the maize genotypes product that the deep root type combined with Soybean Intercropping, root secretion ability are strong, system yield is high Kind, concrete operations are as follows:
A1: selecting corn variety to be screened,
A2: being arranged the planting area with identical Nitrogen Level, and planting area is then divided into nonoculture and makees two ways kind It plants, by corn and soybean planting in corresponding region,
A3: fertilising: corn strip band is before broadcasting and the jointing stage distinguishes applied nitrogen, and soybean band applies starting fertilizer before broadcasting, and big Beans broadcast preceding Rhizobium Inoculation,
A4: uprooting sampling in corn in the spinning phase, takes a picture to sample root system, after analyzed with DIRT software, while taking root system Above ground portion sample disappears and boils survey nitrogen,
A5: maturity period harvest surveys production, and soybean samples in the maturity period, plucks visible root nodule, then counts, air-dries, weighing, certainly with 15N Right abundance method calculates biological nitrogen fixation amount,
A6: go out combine with Soybean Intercropping by indoor water planting experiment sieving most suitable deep root type, root secretion ability by force, system The high maize genotype of yield;
B, using deep root, the corn variety and Soybean Intercropping of shallow root, the physiology of maize/soybean Intercropping System nitrogen efficiency variance is analyzed Mechanism improves maize/soybean Wheat yield nitrogen efficiency maize genotype to filter out,
B1: by the corn variety of the different root systems filtered out in a6 respectively with Soybean Intercropping, and be arranged two kinds of intercropping modes,
B2: three different Nitrogen Level planting areas of height in setting, each Nitrogen Level planting area are arranged two inter-species and make Mode,
B3: in middle high nitrogen fertilizer horizontal zone, corn strip band nitrogenous fertilizer is before broadcasting and the jointing stage is administered twice, and soybean band is before broadcasting Apply starting fertilizer, and the Rhizobium Inoculation before soybean is broadcast;
B4: corn is respectively at the jointing stage, big bell mouth stage, and spinning phase and maturity period uproot sampling, while taking overground part sample, disappears Survey nitrogen is boiled, soil nitrate-N and ammonium nitrogen content are detected,
B5: finally harvest, which is surveyed, produces;It is selected between fine day morning 9:00 ~ 11:00 using LI-6400 type photosynthesizer simultaneously Take the measuring blade Net Photosynthetic Rate of corn;
B6: soybean samples in the maturity period, plucks visible root nodule, counts, air-dries, weighing, calculates biological nitrogen fixation with 15N natural abundance method Amount;To determine the maize genotype that maize/soybean Intercropping System nitrogen efficiency can be improved according to amount of nitrogen fixation.
The method that screening according to the present invention improves maize/soybean Wheat yield nitrogen efficiency maize genotype, into The optimal technical scheme of one step is that Nitrogen Level described in a2 is 150kg/hm2
The method that screening according to the present invention improves maize/soybean Wheat yield nitrogen efficiency maize genotype, into The optimal technical scheme of one step is that the corn nest spacing of nonoculture specification described in a2 is 25cm, and line-spacing is 40cm × 120cm wide-and narrow-row, Corn and Soybean Intercropping line-spacing are 30cm, soybean spacing in the rows 25cm, cave program request 6-8.
The method that screening according to the present invention improves maize/soybean Wheat yield nitrogen efficiency maize genotype, into The optimal technical scheme of one step is: the planting density in planting area is 50,000 plants/hm2
The method that screening according to the present invention improves maize/soybean Wheat yield nitrogen efficiency maize genotype, into The optimal technical scheme of one step is to distinguish applied nitrogen with the jointing stage before corn strip strip sowing in a3, and the amount of application of nitrogenous fertilizer is identical.
The method that screening according to the present invention improves maize/soybean Wheat yield nitrogen efficiency maize genotype, into The optimal technical scheme of one step is that solution culture is specifically that corn variety is carried out water planting in a6, by the training in basin after culture 3 weeks Nutrient solution crosses macroporous resin column and collects root secretion, is then eluted with 85% alcohol to the flavone compound in resin, will The eluent being collected into is concentrated by nitrogen evaporator, measures flavone compound with HPLC, measurement wavelength is 280nm.
The method that screening according to the present invention improves maize/soybean Wheat yield nitrogen efficiency maize genotype, into The optimal technical scheme of one step is that when detecting soil nitrate-N and ammonium nitrogen content in b4, every 30cm soil sampling takes native depth For 0-90cm.
The method that screening according to the present invention improves maize/soybean Wheat yield nitrogen efficiency maize genotype, into The optimal technical scheme of one step is, when the soybean mature phase samples in b6, lifts whole 5 plants of strain semen viciae fabae of shoveling taking-up with iron and is placed in ox On mulberry paper, to guarantee that all root nodules take out, gently shake off the soil on root, collection falls in the root nodule on paper and the root with root nodule, and The soil shaken off is put back at former sampling, sample is placed on flowing water in the sieve of 100 mesh after fetching and rinsed by sample.
The method that screening according to the present invention improves maize/soybean Wheat yield nitrogen efficiency maize genotype, into The optimal technical scheme of one step is that two in b1 kind intercropping mode, wherein corn and soybean line-spacing are in intercropping mode one 30cm, corn and soybean line-spacing are 40cm in intercropping mode two, and maize culture specification nest spacing is 25cm, line-spacing be 40cm × 120cm wide-and narrow-row, soybean spacing in the rows 25cm, cave program request 6-8, when final singling, every cave stayed 5 plants.
The method that screening according to the present invention improves maize/soybean Wheat yield nitrogen efficiency maize genotype, into The optimal technical scheme of one step is that planting density is 50,000 plants/hm in two in b1 kind intercropping mode2
The present invention has the corn variety of different root system configurations and root secretion by screening, studies the underground interaction of nonoculture system Mechanism and pair between make soybean nitrogen-fixing efficiency influence, be clearly suitable for the root system of the corn variety of maize/soybean Wheat yield The whole nitrogen efficiency of Wheat yield can be improved in Morphology And Physiology feature.
Detailed description of the invention
Fig. 1: one schematic diagram of cropping pattern made between maize/soybean in embodiment.
Fig. 2: two schematic diagram of cropping pattern made between maize/soybean in embodiment.
Specific embodiment
The present invention combines specific actual environment below, is done by experiment to technical solution of the present invention further detailed Description.
Embodiment
The present embodiment, which mainly passes through two big steps, to be completed.
One: most suitable deep root type, the corn-based that root secretion ability is strong, system yield is high are combined in screening with Soybean Intercropping Because of type, mainly completed by following;
Experimental design:
15-20 Sichuan is selected to promote mainly corn variety, 1 Nitrogen Level (150kg/hm of test setting in recent years2), including nonoculture Make two kinds of planting patterns (such as Fig. 1) with.Four repetitions of each processing, 16 m of plot area2(3.2m × 5m), maize culture rule Lattice nest spacing is 25cm, and line-spacing is wide-and narrow-row (40cm × 120cm), and corn and Soybean Intercropping line-spacing are 30cm.Each growth belt kind 2 rows are planted, planting density is 50,000 plants/hm2.Soybean spacing in the rows 25cm, cave program request 6-8, when final singling, every cave stayed 5 plants.Corn strip band nitrogen Fertile application in two dressings (before broadcasting: the jointing stage=1:1) applies starting fertilizer 35kg/hm before soybean strip sowing2, inoculation root nodule before soybean is broadcast Bacterium.
Measurement item and technical method:
Corn is uprooted sampling in the spinning phase, and every cell chooses representational 3 plants.Firstly, digging one centered on corn basal part of stem A diameter is 35 centimetres, and the earth pillar that depth is 30 centimetres, maize root system digs out rear slight shake, removes what part loosened with hand Root is immersed in mild detergent by soil;Finally, still sticking together a small amount of soil on root system with the flushing of low pressure hydraulic giant. After root system dries, root system photograph is carried out, root system photographic means is with reference to (Bucksch et al., 2014).Use one 80 lis The high square film studio (DEEP board, Beijing white light photographic goods company) of rice, camera (SONYILCE 5100) is placed in photography Ceiling canopy middle, root system are placed on film studio center after drying, root system is analyzed after taking pictures with DIRT software.It takes simultaneously on the ground Portion's sample disappears and boils survey nitrogen.Maturity period harvest, which is surveyed, to be produced.Soybean samples in the maturity period, digs about 0.60 m with spade and takes out whole strain silkworm deeply 5 plants of beans are placed on brown paper, to guarantee that all root nodules take out, gently shake off the soil on root, collect the root nodule and band fallen on paper The root of root nodule, and the soil shaken off is put back at former sampling.Sample is placed on flowing water in the sieve of 100 mesh after fetching and rinsed by sample, is plucked It can be seen that root nodule, counting, air-dried, weighing.Biological nitrogen fixation amount is calculated with 15N natural abundance method.
The strong genotype of indoor water planting experiment sieving root secretion ability: 15-20 corn variety is subjected to water planting, culture The culture solution in basin is crossed into macroporous resin column after 3 weeks and collects root secretion, then with 85% alcohol to the flavonoids in resin It closes object to be eluted, the eluent being collected into is concentrated by nitrogen evaporator, measure flavone compound with HPLC, measure wave A length of 280nm.
In summary test result filters out and combines that most suitable deep root type, root secretion ability be strong, body with Soybean Intercropping It is the high maize genotype of yield.
Two: using deep root, the maize genotype and Soybean Intercropping of shallow root, analyzing maize/soybean Intercropping System nitrogen inefficient Different physiological mechanism.
Experimental design:
The maize genotype (deep root 1, deep root 2, shallow root 1 and shallow root 2) of the different root system sizes of 4 that the first step is filtered out with it is big Make between beans.Test 3 Nitrogen Levels (0,150,300kg/hm of setting2), two kinds of intercropping modes are arranged in each Nitrogen Level, work Corn and soybean line-spacing are 30cm(such as Fig. 1) in mode one, and corn and soybean line-spacing are 40cm(such as Fig. 2 in intercropping mode two). Four repetitions of each processing, 16 m of plot area2(3.2m × 5m), maize culture specification nest spacing are 25cm, and line-spacing is wide-and narrow-row (40cm × 120cm), each growth belt plant 2 rows, and planting density is 50,000 plants/hm2.Soybean spacing in the rows 25cm, cave program request 6-8, Every cave stays 5 plants when final singling.In 150 and 300kg/hm2In N fertilization, corn strip band nitrogenous fertilizer application in two dressings is (before broadcasting: jointing Phase=1:1), starting fertilizer 35kg/hm is applied before soybean strip sowing2, soybean broadcasts preceding Rhizobium Inoculation.
Measurement item and technical method:
Corn is in the jointing stage, big bell mouth stage, and spinning phase and maturity period uproot sampling, and sampling method is the same as content one.Take ground simultaneously Upper sample disappears and boils survey nitrogen, and every 30cm soil sampling, takes 0-90cm depth, detects soil nitrate-N and ammonium nitrogen content.Finally Harvest, which is surveyed, to be produced.Simultaneously using LI-6400 type photosynthesizer (LI-COR, the U.S.) between fine day morning 9:00 ~ 11:00 Choose measuring blade Net Photosynthetic Rate (μm ol CO2/m of 5 plants of corns of label2/ s).
Soybean samples in the maturity period.It lifts about 0.50 m of digging whole 5 plants of strain semen viciae fabae of taking-up deeply with iron to be placed on brown paper, to protect It demonstrate,proves all root nodules to take out, gently shakes off the soil on root, collection falls in the root nodule on paper and the root with root nodule, and the soil shaken off is put It returns at former sampling.Sample is placed on flowing water in the sieve of 100 mesh after fetching and rinsed by sample, is plucked visible root nodule, counting, is air-dried, claims Weight.Biological nitrogen fixation amount is calculated with 15N natural abundance method.
In summary test result clearly improves the crucial corn root dry mass and root of maize/soybean Intercropping System nitrogen efficiency It is the critical period of interaction.
From above-mentioned it is big that raising corn can be filtered out reasonablely in conjunction with analysis of experiments data with test result The maize genotype of nitrogen efficiency in the system made between beans improves the income of farmer to improve agricultural planting efficiency, increases warp Ji benefit.
For test area selected by the present embodiment in Sichuan, those skilled in the art according to the actual situation, can be by area Any region is revised as in domain, in conjunction with local actual environment, test accordingly.
Based on the above embodiment in the present invention, those of ordinary skill in the art are not making creative work premise Under every other embodiment obtained shall fall within the protection scope of the present invention.Therefore, embodiments of the present invention is detailed Description is not intended to limit the range of claimed invention, but is merely representative of selected embodiment of the invention.

Claims (10)

1. a kind of method that screening improves maize/soybean Wheat yield nitrogen efficiency maize genotype, it is characterised in that: including following Operation:
A, screening is suitble to the maize genotypes product that the deep root type combined with Soybean Intercropping, root secretion ability are strong, system yield is high Kind, concrete operations are as follows:
A1: selecting corn variety to be screened,
A2: being arranged the planting area with identical Nitrogen Level, and planting area is then divided into nonoculture and makees two ways kind It plants, by corn and soybean planting in corresponding region,
A3: fertilising: corn strip band is before broadcasting and the jointing stage distinguishes applied nitrogen, and soybean band applies starting fertilizer before broadcasting, and big Beans broadcast preceding Rhizobium Inoculation,
A4: uprooting sampling in corn in the spinning phase, takes a picture to sample root system, after analyzed with DIRT software, while taking root system Above ground portion sample disappears and boils survey nitrogen,
A5: maturity period harvest surveys production, and soybean samples in the maturity period, plucks visible root nodule, then counts, air-dries, weighing, certainly with 15N Right abundance method calculates biological nitrogen fixation amount,
A6: go out combine with Soybean Intercropping by indoor water planting experiment sieving most suitable deep root type, root secretion ability by force, system The high maize genotype of yield;
Using deep root, the corn variety and Soybean Intercropping of shallow root, the physiology machine of maize/soybean Intercropping System nitrogen efficiency variance is analyzed System improves maize/soybean Wheat yield nitrogen efficiency maize genotype to filter out,
B1: by the corn variety of the different root systems filtered out in a6 respectively with Soybean Intercropping, and be arranged two kinds of intercropping modes,
B2: three different Nitrogen Level planting areas of height in setting, each Nitrogen Level planting area are arranged two inter-species and make Mode,
B3: in middle high nitrogen fertilizer horizontal zone, corn strip band nitrogenous fertilizer is before broadcasting and the jointing stage is administered twice, and soybean band is before broadcasting Apply starting fertilizer, and the Rhizobium Inoculation before soybean is broadcast;
B4: corn is respectively at the jointing stage, big bell mouth stage, and spinning phase and maturity period uproot sampling, while taking overground part sample, disappears Survey nitrogen is boiled, soil nitrate-N and ammonium nitrogen content are detected,
B5: finally harvest, which is surveyed, produces;It is selected between fine day morning 9:00 ~ 11:00 using LI-6400 type photosynthesizer simultaneously Take the measuring blade Net Photosynthetic Rate of corn;
B6: soybean samples in the maturity period, plucks visible root nodule, counts, air-dries, weighing, calculates biological nitrogen fixation with 15N natural abundance method Amount;To determine the maize genotype that maize/soybean Intercropping System nitrogen efficiency can be improved according to amount of nitrogen fixation.
2. the method that screening according to claim 1 improves maize/soybean Wheat yield nitrogen efficiency maize genotype, special Sign is that Nitrogen Level described in a2 is 150kg/hm2
3. the method that screening according to claim 1 improves maize/soybean Wheat yield nitrogen efficiency maize genotype, special Sign is that the corn nest spacing of nonoculture specification described in a2 is 25cm, and line-spacing is 40cm × 120cm wide-and narrow-row, corn and Soybean Intercropping Line-spacing is 30cm, soybean spacing in the rows 25cm, cave program request 6-8.
4. the method that screening according to claim 1 improves maize/soybean Wheat yield nitrogen efficiency maize genotype, special Sign is that the planting density in planting area is 50,000 plants/hm2
5. the method that screening according to claim 1 improves maize/soybean Wheat yield nitrogen efficiency maize genotype, special Sign is, distinguishes applied nitrogen with the jointing stage before corn strip strip sowing in a3, and the amount of application of nitrogenous fertilizer is identical.
6. the method that screening according to claim 1 improves maize/soybean Wheat yield nitrogen efficiency maize genotype, special Sign is that solution culture is specifically that corn variety is carried out water planting in a6, and the culture solution in basin is crossed macroreticular resin after culture 3 weeks Column collects root secretion, is then eluted with 85% alcohol to the flavone compound in resin, the eluent that will be collected into It is concentrated by nitrogen evaporator, measures flavone compound with HPLC, measurement wavelength is 280nm.
7. the method that screening according to claim 1 improves maize/soybean Wheat yield nitrogen efficiency maize genotype, special Sign is, when detecting soil nitrate-N and ammonium nitrogen content in b4, every 30cm soil sampling, taking native depth is 0-90cm.
8. the method that screening according to claim 1 improves maize/soybean Wheat yield nitrogen efficiency maize genotype, special Sign is, when the soybean mature phase samples in b6, lifts whole 5 plants of strain semen viciae fabae of shoveling taking-up with iron and is placed on brown paper, to guarantee There is root nodule taking-up, gently shakes off the soil on root, collection falls in the root nodule on paper and the root with root nodule, and the soil shaken off is put back to original At sampling, sample is placed on flowing water in the sieve of 100 mesh after fetching and rinsed by sample.
9. the method that screening according to claim 1 improves maize/soybean Wheat yield nitrogen efficiency maize genotype, special Sign is, two in b1 kind intercropping mode, and wherein corn and soybean line-spacing are 30cm in intercropping mode one, in intercropping mode two Corn and soybean line-spacing are 40cm, and maize culture specification nest spacing is 25cm, and line-spacing is 40cm × 120cm wide-and narrow-row, soybean spacing in the rows 25cm, cave program request 6-8, when final singling, every cave stayed 5 plants.
10. the method that screening according to claim 1 improves maize/soybean Wheat yield nitrogen efficiency maize genotype, It is characterized in that, planting density is 50,000 plants/hm in two in b1 kind intercropping mode2
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