CN109304366A - A kind of amino acid water-soluble fertilizer is in the application for strengthening heavy metal accumulation plant extract serious pollution Cadmium in Soil - Google Patents
A kind of amino acid water-soluble fertilizer is in the application for strengthening heavy metal accumulation plant extract serious pollution Cadmium in Soil Download PDFInfo
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- CN109304366A CN109304366A CN201811105350.9A CN201811105350A CN109304366A CN 109304366 A CN109304366 A CN 109304366A CN 201811105350 A CN201811105350 A CN 201811105350A CN 109304366 A CN109304366 A CN 109304366A
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B09—DISPOSAL OF SOLID WASTE; RECLAMATION OF CONTAMINATED SOIL
- B09C—RECLAMATION OF CONTAMINATED SOIL
- B09C1/00—Reclamation of contaminated soil
- B09C1/10—Reclamation of contaminated soil microbiologically, biologically or by using enzymes
- B09C1/105—Reclamation of contaminated soil microbiologically, biologically or by using enzymes using fungi or plants
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B09—DISPOSAL OF SOLID WASTE; RECLAMATION OF CONTAMINATED SOIL
- B09C—RECLAMATION OF CONTAMINATED SOIL
- B09C2101/00—In situ
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Abstract
The present invention provides a kind of amino acid water-soluble fertilizers in the application for strengthening heavy metal accumulation plant extract serious pollution Cadmium in Soil, belongs to environmental protection and ecological restoration technical field.By planting Cd accumulation plant in cadmium serious pollution soil, after mature plant growth, amino acid water-soluble fertilizer (AA) is poured to plant root.After plant strain growth 40 ~ 60 days, by harvesting plant, especially overground part, the removal of soil heavy metal cadmium (Cd) is realized.Present invention firstly provides novel amino acid water-soluble fertilizer is applied to enhancing heavy metal accumulation plant to the high efficiency extraction of severe contaminated soil cadmium, a kind of efficient, low in cost, environmental-friendly new method is provided for the phytoremediation of severe cadmium pollution soil.
Description
Technical field
The present invention relates to environmental protections and ecological restoration technical field, and in particular to a kind of reinforcing enriching plant extraction severe
The method of contaminated soil cadmium.
Background technique
With the continuous quickening of process of industrialization, due to anthropogenic discharge's waste and waste water, a huge sum of money for soil in recent decades
Belong to pollution to sharply increase.Heavy-metal contaminated soil is not only a serious Global Environmental Problems, also has become China's soil matter
An important factor for amount decline.Forefathers are studies have shown that heavy metal pollution has the characteristics that concealment, generality and non-degradable.Weight
Metal causes greatly to destroy to the almost all of all toxic effect of biology to environment, and can be influenced by food chain dynamic
The health of the object even mankind.Therefore, oneself warp of restoration of soil polluted by heavy metal becomes the hot spot of various circles of society's research.
Common heavy metal restorative procedure includes peripheral doses, chemical remediation and biological prosthetic.Physical chemistry reparation has electricity
Chemical method, thermal desorption method, vitrification, casting landfill method, elution method, dilution method etc..It is biological prosthetic include phytoremediation, it is dynamic
Object reparation and microorganism remediation etc..Physics and chemical remediation technology can quickly repair the soil by heavy metal pollution, but price
Function and characteristic expensive and that soil can be interfered.And it is biological prosthetic can be used in combination with other technologies, and have expense low, no
Easily cause the advantages such as secondary pollution.Phytoremediation is as a kind of green environmental protection technique for repairing toxic heavy metal contamination, using most
It is extensive.
The effects of phytoremediation includes plant stability, phytovolatilization, plant extract.But phytoremediation is research hotspot master
Concentrate on the super tired plant extraction technology for the purpose of removing removing heavy metals.But when the heavy metal concentration accumulated in plant is excessively high
Plant tissue can be made to generate irreversible injury, cause plant growth ability to decline, result even in death.Therefore, if thinking
Plant remediation ability is improved, the task of top priority is to improve plant growth ability and to Accumulation of heavy metals ability.Then external source is proposed
The method of the plant combined reparation of additive-, the extraction using external source reinforced by additive plant to heavy metal, such as microbial bacterial agent and
The chelating agents such as ethylenediamine tetra-acetic acid (EDTA), ethylenediamine disuccinic acid (EDDS), nitrilotriacetic acid (NTA).However, as people grind
That studies carefully gos deep into, currently used outer source additive there is also high expensive, sequestering power is poor, effective acting time is short, itself
There are the problems such as secondary pollution, are restricted popularization and application, so as to cause enriching plant to Heavy Metals in Soil Contaminated
Extraction efficiency is not high.
Summary of the invention
In order to overcome drawbacks described above, the object of the present invention is to provide a kind of reinforcing enriching plants to extract serious pollution Cadmium in Soil
Method, by heavy Cd contamination grown on soil to cadmium have strong accumulation ability black nightshade, after plant grows up, to root
It carries out implementing amino acid water-soluble fertilizer, promotes absorption of the black nightshade to heavy metal in soil, it, will when plant strain growth was to 40~60 days
Black nightshade removes from contaminated soil.This method can promote the suction of plant growth and plant to heavy metal in soil
It receives.
Amino acid water-soluble fertilizer mainly discards pig as a kind of new-type fertilizer that biomass energy recycles with slaughterhouse
Hair, livestock and poultry feather are that raw material is chelated other microelements and be made by high temperature acidolysis.About amino acid water-soluble fertilizer in agricultural
The existing many reports of application in production, have focused largely on and promote plant growth, enhancing crop photosynthesis efficiency, improve the anti-of plant
Pest and disease damage ability, the ability for resisting the environment-stress such as salt marsh and arid；The yield and quality of crop can also be effectively improved.And by ammonia
The molten fertilizer application of base sour water does not have been reported that also in the phytoremediation of heavy Cd contamination soil.Because amino acid water-soluble fertilizer greatly improves
Phytomass, can remarkably promote enriching plant to the high efficiency extraction of Cd in severe contaminated soil；Its is low in cost simultaneously,
Realize the cycling and reutilization of biomass energy；Therefore amino acid water-soluble fertilizer is applied to enriching plant repairing heavy metal pollution soil
Earth has a good application prospect.
In order to achieve the above object, the technical scheme adopted by the invention is that:
A kind of amino acid water-soluble fertilizer in the application for strengthening heavy metal accumulation plant extract serious pollution Cadmium in Soil, including under
Column step:
1) cadmium serious pollution area 0-20cm surface soil is taken, and air-dried, ground, is sieved (20 mesh)；
2) cultivate black nightshade seedling in laboratory conditions, when growth of seedling to three leaves wholeheartedly when, select growing way healthy and strong consistent
Seedling replanting enter in plastic flowerpot；
3) after black nightshade seedling stablizes 10~15 days, amino acid water-soluble fertilizer (AA) is applied in filling；
4) plant is harvested after black nightshade growth of seedling 40~60 days, measures biomass and the content of plant each section Cd.
According to above scheme step 1), the content of Cd is 8.21mgkg in the soil in cadmium pollution area-1, pH 7.21.
According to above scheme step 2), the step of cultivating black nightshade seedling are as follows: black fruit black nightshade seed is sterilized, is cleaned, drift removes
Shrivelled kernel, it is spare after natural air drying；The vermiculite of sterilizing is laid in pallet, black nightshade seed is seeded in vermiculite surface layer, above covers thin layer leech
Stone；The vermiculite pallet for containing black nightshade seed is placed in incubator, and pallet is put into culturing room after black nightshade seed germination.More specifically
Cultivate the detailed process of black nightshade seedling in ground are as follows: black fruit black nightshade seed is sterilized into 20min, distilled water punching with 0.5%NaClO solution
Wash clean floats and removes shrivelled kernel, spare after natural air drying.Then the vermiculite of sterilizing is laid in pallet, thickness 4-6cm, black nightshade seed
It is seeded in wherein.The vermiculite pallet for containing black nightshade seed is placed in incubator, and pallet is put into culturing room after black nightshade seed germination.
Natural lighting is simulated using fluorescent tube by culturing room, and day-night cycle is 16h illumination, and 8h is dark, and relative humidity control is on 80% left side
It is right.
According to above scheme step 3), amino acid fertilizer used is to utilize the discarded micro member of pig hirsutic acid solution chelating amino acids
Made of element, nitrogen, phosphorus, potassium (N, P2O5、K2O) nutrient content is respectively 29.7gkg-1、6.7g·kg-1And 22.7gkg-1.Amino acid fertilizer concentration is 50 times of dilution, and every young plant pours amino acid fertilizer after 25mL dilution.And amino acid fertilizer after diluting,
Its nitrogen, phosphorus, potassium (N, P2O5、K2O) nutrient content is respectively 29.7/50gkg-1、6.7/50g·kg-1And 22.7/50gkg-1。
According to above scheme step 4), the method for the measurement concentrated acid wet digestion of cadmium content in plant: excellent pure grade is used
Nitric acid and perchloric acid according to volume 87:13 be made into nitration mixture, cold resolution overnight, is subsequently placed on electric heating resolution instrument gradually under room temperature
It is warming up to 160-180 DEG C, until being evaporated.
Compared with existing restorative procedure and technology, the beneficial effects of the present invention are:
(1) the amino acid water-soluble fertilizer that the present invention utilizes strengthens the method and skill that enriching plant extracts serious pollution Cadmium in Soil
Art substantially increases plant to the absorbability of heavy metal.
(2) the amino acid water-soluble fertilizer that the present invention utilizes can promote plant growth and contaminated soil to provide nitrogen, phosphorus
Element has the function of soil infertile field.
(3) raw material for the amino acid water-soluble fertilizer that the present invention utilizes is cheap, not only saves phytoremediation heavy metal polluted soil
The cost of earth, while waste has also been handled, so that waste is reused, it is significant to promotion agricultural sustainable development.
Detailed description of the invention
Fig. 1 is control group (not applying amino acid water-soluble fertilizer) and is applied with the black nightshade of the test group of amino acid water-soluble fertilizer
Overground part and root system Cd content schematic diagram.
Specific embodiment
Below with reference to specific example, the present invention will be further described.
Embodiment 1
1, for trying soil: soil is derived from Pb-Zn deposits surrounding area, Nanjing 0-20cm surface soil, is air-dried, is ground, mistake
It is stand-by afterwards to sieve (20 mesh).The content of Cd is more than 8 times of national grade III Standard in contaminated soil, is 8.21mgkg-1, pH is
7.21。
2, the preparation of black nightshade seedling: black fruit black nightshade seed is sterilized into 20min with 0.5%NaClO solution, distilled water flushing is dry
Only, it floats and removes shrivelled kernel, it is spare after natural air drying.Then the vermiculite of sterilizing is laid in pallet, thickness 4-6cm, black nightshade seed sowing
In wherein.The vermiculite pallet for containing black nightshade seed is placed in incubator, and pallet is put into culturing room after black nightshade seed germination.Culture
Natural lighting is simulated using fluorescent tube in room, and day-night cycle is 16h illumination, and 8h is dark, and relative humidity is controlled 80% or so.To
When seedling grows to three leaves wholeheartedly, the healthy and strong consistent seedling replanting of growing way is selected to enter plastic flowerpot (bore 15cm).
3, pot experiment: it is packed into 1.0kg in every basin for examination cadmium pollution soil, transplants after black nightshade root is cleaned up to flowerpot
In, 5 plants of every pot transplanting.Culture room is put into after transplanting, natural lighting is simulated using fluorescent tube in culture room, and temperature control exists
20-25 DEG C, daily illumination 10h, relative humidity controls between 70-80%, and watering is to keep water capacity for 60% or so.
It applied fertilizer to plant strain growth 15 days, amino acid fertilizer is diluted into 50 times of backward plant roots and is poured.Control group is with the water of equivalent
It is poured, 4 repetitions are arranged in each processing.Black nightshade grows to 40 days and harvests, and surveys its biomass and each section cadmium content respectively.
4, content of beary metal detects: the measurement of cadmium (Cd) uses concentrated acid wet digestion in plant, using excellent pure grade nitric acid and
Perchloric acid is made into nitration mixture according to volume 87:13, weighs 0.2000 ± 0.0050g Plant samples and (is divided into plant on the ground before weighing
Portion and the underground part continuous drying 10h or more in 80 DEG C of baking ovens, until constant weight), 3mL nitration mixture is added, cold resolution is (big overnight under room temperature
About 10h), it is gradually then warming up to 160-180 DEG C with electric heating resolution instrument, until being evaporated, then the excellent of 10mL 2.5% is accurately added
The pure nitric acid (w/v) of grade.Be vortexed and be then vortexed again primary to precipitating dissolution and in 60 DEG C of water-bath 30min, staticly settle overnight or
After centrifugation, supernatant is transferred in 10mL centrifuge tube for use.It uses inductive coupling plasma emission spectrograph (ICP-OES)
Measure the content of cadmium in Plant samples.
5, as shown in Table 1, compared with control group (not applying amino acid water-soluble fertilizer), it is applied with amino acid water-soluble fertilizer
307.4% and 378.1% has been respectively increased in test group black nightshade overground part and root biomass (dry weight).
Influence of the 1 amino acid water-soluble fertilizer of table to black nightshade biomass
Influence of 2 amino acid fertilizer of embodiment to black nightshade Cd content
Experimental method is with embodiment 1, as a result as shown in table 2 and Fig. 1, compared with the control, is applied with amino acid water-soluble fertilizer
Test group black nightshade overground part and root system Cd content 397.1% and 283.9% has been respectively increased, greatly improve black nightshade pair
The absorption of Cd in soil.Also, by table 3 it can be concluded that amino acid water-soluble fertilizer makes the extraction of black nightshade overground part and underground part Cd
Amount all improves nearly 18 times, illustrates that it is feasible for strengthening black nightshade using the fertilizer to extract the technology of contaminated soil cadmium.
Influence of the 2 amino acid water-soluble fertilizer of table to Cd content in black nightshade
3 amino acid water-soluble fertilizer of table extracts the influence of contaminated soil Cd to black nightshade
It should be noted that the above embodiments do not limit the invention in any form, it is all to use equivalent replacement or equivalent change
The mode changed technical solution obtained, falls within the scope of protection of the present invention.
Claims (6)
1. a kind of amino acid water-soluble fertilizer is in the application for strengthening heavy metal accumulation plant extract serious pollution Cadmium in Soil, including as follows
Step:
Cadmium serious pollution area 0-20cm surface soil is taken, and is air-dried, ground, is sieved；
Cultivate black nightshade seedling, when growth of seedling to three leaves wholeheartedly when, select the healthy and strong consistent seedling replanting of growing way to enter plastic flowerpot
In；
After black nightshade seedling activates 10 ~ 15 days, amino acid water-soluble fertilizer (AA) is applied in filling；
Plant is harvested after black nightshade growth of seedling 40 ~ 60 days, measures biomass and the content of plant each section Cd.
2. amino acid water-soluble fertilizer according to claim 1 is strengthening heavy metal accumulation plant extract serious pollution Cadmium in Soil
Application, it is characterised in that: in step 1), in the soil in cadmium serious pollution area the content of Cd be 8.21 mgkg-1, pH
It is 7.21.
3. amino acid water-soluble fertilizer according to claim 1 is strengthening heavy metal accumulation plant extract serious pollution Cadmium in Soil
Application, it is characterised in that: in step 2, cultivate black nightshade seedling detailed process are as follows: to black fruit black nightshade seed sterilize, wash
Only, it floats and removes shrivelled kernel, it is spare after natural air drying；The vermiculite of sterilizing is laid in pallet, black nightshade seed is seeded in vermiculite surface layer, above covers
Thin layer vermiculite；The vermiculite pallet for containing black nightshade seed is placed in incubator, and pallet is put into culturing room after black nightshade seed germination.
4. amino acid water-soluble fertilizer according to claim 1 is strengthening heavy metal accumulation plant extract serious pollution Cadmium in Soil
Application, it is characterised in that: in step 2, with 0.5% NaClO solution by black fruit black nightshade seed sterilize 20 min, distilled water
It rinses well, floats and remove shrivelled kernel, it is spare after natural air drying；Then the vermiculite of sterilizing is laid in pallet, vermiculite layers thickness 4-6 cm,
Black nightshade seed is seeded in vermiculite surface layer, above covers thin layer vermiculite；The vermiculite pallet for containing black nightshade seed is placed in incubator, black nightshade kind
Pallet is put into culturing room after son germination；Natural lighting, day-night cycle: 16 h illumination, 8 h are simulated using fluorescent tube by culturing room
Dark, relative humidity are controlled 80% or so.
5. amino acid water-soluble fertilizer according to claim 1 is strengthening heavy metal accumulation plant extract serious pollution Cadmium in Soil
Application, it is characterised in that: in step 3), amino acid fertilizer used is micro using discarded pig hirsutic acid solution chelating amino acids
Made of element, nitrogen, phosphorus, potassium (N, P2O5、K2O) nutrient content is respectively 29.7 gkg-1、6.7 g·kg-1With
22.7g·kg-1；In use, amino acid fertilizer dilutes 50 times, every plant of black nightshade seedling pours amino acid fertilizer after 25mL dilution.
6. amino acid water-soluble fertilizer according to claim 1 is strengthening heavy metal accumulation plant extract serious pollution Cadmium in Soil
Application, it is characterised in that: in step 4), in plant cadmium content measurement use concentrated acid wet digestion method: use top grade
Pure nitric acid and perchloric acid is made into nitration mixture according to volume 87: 13, and cold resolution overnight, is subsequently placed in electric heating resolution instrument under room temperature
In be gradually warming up to 160-180 DEG C, until be evaporated.
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| CN110252801A (en) * | 2019-07-12 | 2019-09-20 | 南京农业大学 | A kind of method of cadmium pollution soil repair |
| CN114029338A (en) * | 2021-11-08 | 2022-02-11 | 上海交通大学 | Optimized restoration method for black nightshade in moderate cadmium polluted soil |
| CN114749478A (en) * | 2022-04-02 | 2022-07-15 | 河南中烟工业有限责任公司 | Method for restoring lead-polluted soil |
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Application publication date: 20190205 |