CN109287883A - A kind of laying poultry composite fermented feed additive, preparation method and application - Google Patents
A kind of laying poultry composite fermented feed additive, preparation method and application Download PDFInfo
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- CN109287883A CN109287883A CN201811117771.3A CN201811117771A CN109287883A CN 109287883 A CN109287883 A CN 109287883A CN 201811117771 A CN201811117771 A CN 201811117771A CN 109287883 A CN109287883 A CN 109287883A
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- fermented feed
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- 239000008223 sterile water Substances 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
- 239000006228 supernatant Substances 0.000 description 1
- 239000013589 supplement Substances 0.000 description 1
- 238000003786 synthesis reaction Methods 0.000 description 1
- 238000010998 test method Methods 0.000 description 1
- 235000019157 thiamine Nutrition 0.000 description 1
- KYMBYSLLVAOCFI-UHFFFAOYSA-N thiamine Chemical compound CC1=C(CCO)SCN1CC1=CN=C(C)N=C1N KYMBYSLLVAOCFI-UHFFFAOYSA-N 0.000 description 1
- 229960003495 thiamine Drugs 0.000 description 1
- 239000011721 thiamine Substances 0.000 description 1
- 235000013619 trace mineral Nutrition 0.000 description 1
- 239000011573 trace mineral Substances 0.000 description 1
- 210000003462 vein Anatomy 0.000 description 1
- 229940088594 vitamin Drugs 0.000 description 1
- 229930003231 vitamin Natural products 0.000 description 1
- 235000013343 vitamin Nutrition 0.000 description 1
- 235000019155 vitamin A Nutrition 0.000 description 1
- 239000011719 vitamin A Substances 0.000 description 1
- 235000019166 vitamin D Nutrition 0.000 description 1
- 239000011710 vitamin D Substances 0.000 description 1
- 150000003710 vitamin D derivatives Chemical class 0.000 description 1
- 235000019165 vitamin E Nutrition 0.000 description 1
- 229940046009 vitamin E Drugs 0.000 description 1
- 239000011709 vitamin E Substances 0.000 description 1
- 235000019168 vitamin K Nutrition 0.000 description 1
- 239000011712 vitamin K Substances 0.000 description 1
- 150000003721 vitamin K derivatives Chemical class 0.000 description 1
- 229940045997 vitamin a Drugs 0.000 description 1
- 229940046008 vitamin d Drugs 0.000 description 1
- 150000003722 vitamin derivatives Chemical class 0.000 description 1
- 229940046010 vitamin k Drugs 0.000 description 1
- 238000005406 washing Methods 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K50/00—Feeding-stuffs specially adapted for particular animals
- A23K50/70—Feeding-stuffs specially adapted for particular animals for birds
- A23K50/75—Feeding-stuffs specially adapted for particular animals for birds for poultry
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K10/00—Animal feeding-stuffs
- A23K10/10—Animal feeding-stuffs obtained by microbiological or biochemical processes
- A23K10/12—Animal feeding-stuffs obtained by microbiological or biochemical processes by fermentation of natural products, e.g. of vegetable material, animal waste material or biomass
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K10/00—Animal feeding-stuffs
- A23K10/10—Animal feeding-stuffs obtained by microbiological or biochemical processes
- A23K10/14—Pretreatment of feeding-stuffs with enzymes
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K10/00—Animal feeding-stuffs
- A23K10/20—Animal feeding-stuffs from material of animal origin
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K10/00—Animal feeding-stuffs
- A23K10/20—Animal feeding-stuffs from material of animal origin
- A23K10/22—Animal feeding-stuffs from material of animal origin from fish
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K10/00—Animal feeding-stuffs
- A23K10/30—Animal feeding-stuffs from material of plant origin, e.g. roots, seeds or hay; from material of fungal origin, e.g. mushrooms
- A23K10/37—Animal feeding-stuffs from material of plant origin, e.g. roots, seeds or hay; from material of fungal origin, e.g. mushrooms from waste material
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K20/00—Accessory food factors for animal feeding-stuffs
- A23K20/10—Organic substances
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K20/00—Accessory food factors for animal feeding-stuffs
- A23K20/10—Organic substances
- A23K20/158—Fatty acids; Fats; Products containing oils or fats
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K20/00—Accessory food factors for animal feeding-stuffs
- A23K20/10—Organic substances
- A23K20/163—Sugars; Polysaccharides
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2400/00—Lactic or propionic acid bacteria
- A23V2400/11—Lactobacillus
- A23V2400/175—Rhamnosus
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
- Y02P60/00—Technologies relating to agriculture, livestock or agroalimentary industries
- Y02P60/80—Food processing, e.g. use of renewable energies or variable speed drives in handling, conveying or stacking
- Y02P60/87—Re-use of by-products of food processing for fodder production
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Abstract
The invention discloses a kind of laying poultry composite fermented feed additive, preparation method and applications, belong to field of feed additive technology.Laying poultry composite fermented feed additive of the invention is prepared in parts by weight by following components: 22~25 parts of sunflower seed dregs of fermentation, fermentation 15~20 parts of algae powder, 2~4 parts of ostreae testa pulverata, 1~3 part of yellow meal worm powder, 0.05~0.1 part of isoflavones, 0.1~0.3 part of carboxymethyl chitosan, 0.05~0.1 part of perilla herb oil.Laying poultry composite fermented feed additive of the invention can not only improve the laying rate of poultry, increase egg laying performance, moreover it is possible to further improve quality of laying eggs；Composite fermented feed additive of the invention is the intestinal environment by adjusting poultry, promotes the growth and breeding of probiotics, and then promote the digestion and absorption of nutriment, improves immunity.
Description
Technical field
The invention belongs to field of feed additive technology, and in particular to a kind of laying poultry is added with fermented composite feed
Agent, preparation method and application.
Background technique
Egg food such as egg, duck's egg, goose egg, quail egg, pigeon egg are the most common diet in people's daily life
One of food, wherein egg is the conventional food on people's dining table.The nutritive value of eggs food is high, wherein yolk contains
The nutritional ingredients such as protein, fat, calcium, lecithin and irony abundant, can not only promote the exclusion of cholesterol in blood vessel, have
Prevention of arterial gruel type also has the ability for promoting study, memory, reaches effect of prevention old man's dementia；Contain one in albumen
Kind is the albumen of albumin, the effect of having the function of Scavenger of ROS, can enhance human immunity, reach anti-cancer.
In order to improve poultry egg laying amount and quality of laying eggs, to meet the needs of people, the technical staff for cultivating field is used
A variety of feeding methods；For example, by the daily schedule of the improvements of terms poultry such as illumination, to improve its laying rate and matter of laying eggs
Amount, cannot but improve quality of laying eggs；For this problem, nutritional supplement is added in feed becomes popular means, such as directly
It is various micro- to connect addition calcium carbonate, potassium iodide, trace mineral supplement, amino acid additive, vitamine supplement etc. in feed
Secondary element and mineral salt, this addition can improve rapidly nutritional ingredient of laying eggs in a short time, can if things go on like this make poultry to feeding
Expect that the nutritious absorption in Central Plains reduces, poultry abilities of digestive and absorption weakens, and a variety of diseases is then caused to occur；In addition, mistake
More addition nutritional supplements not only can be such that excessive ingredient is discharged with excreta, reduce utilization rate；Can also poison be generated to poultry
Evil effect；In recent years, solution to the problem is turned to technical field of Chinese medicine by the technical staff for cultivating field, however, poultry
It is larger with the differences of Physiological of human body, in human body there is the Chinese prescription of better effects to be not necessarily suitable poultry；At present to poultry
The equilibrium between yin and yang and gas-liquid transporting of body are explained there has been no specific, under the guidance of differential diagnosis in tcm thought, a certain Chinese prescription
And do not have general applicability, traditional Chinese medicine ingredients are added in poultry diet to be possible to improve the laying rate and production of poultry in a short time
Egg Quality, it is also possible to desired effects cannot be obtained；The addition of blindness traditional Chinese medicinal components is also possible to lead to poultry physiologic derangement, in turn
There is the problems such as poultry death and quality of laying eggs decline, oviposition period is irregular, large-scale farming industry can not be applied to.
At present, the task of top priority should take based on the digestion and absorption characteristic of poultry itself from the characteristic of poultry
It is equipped with rationally natural feed or additive, complies with normal physiology course, to improve egg production and quality of laying eggs.
Summary of the invention
Aiming at the problems existing in the prior art, the purpose of the present invention is to provide a kind of reasonable nutritional arrangment, can promote
Into poultry abilities of digestive and absorption, the feed addictive, the preparation method that improve immunity and then increase laying rate and quality of laying eggs
And application.
To achieve the goals above, the present invention obtains not technical solution are as follows:
A kind of laying poultry composite fermented feed additive is prepared in parts by weight by following components:
Ferment 22~25 parts of sunflower seed dregs, 15~20 parts of algae powder of fermentation, 2~4 parts of ostreae testa pulverata, 1~3 part of yellow meal worm powder, big
0.05~0.1 part of beans isoflavones, 0.1~0.3 part of carboxymethyl chitosan, 0.05~0.1 part of perilla herb oil.
On the basis of above scheme, the laying poultry composite fermented feed additive, by weight by following components
Number is prepared:
Ferment 22 parts of sunflower seed dregs, fermentation 18 parts of algae powder, 3 parts of ostreae testa pulverata, 2 parts of yellow meal worm powder, 0.06 part of isoflavones,
0.18 part of carboxymethyl chitosan, 0.06 part of perilla herb oil.
On the basis of above scheme, it is described fermentation sunflower seed dregs the preparation method comprises the following steps:
(1) it after sunflower seed dregs remove sundries, is mechanically pulverized, crosses 20 meshes, 121 DEG C of 30min of high pressure steam sterilization have sterilized
Cheng Hou is cooled to room temperature；
(2) moisture content for adjusting sunflower seed dregs after sterilizing is 55~65%, and 7~10% aspergillus oryzaes are inoculated with into peanut meal
With the spore suspension of 5~8% type neurosporas, after being sufficiently mixed, carry out solid state fermentation, 28 DEG C~32 DEG C, ferment 36h after, then
For 24 hours in 32~35 DEG C of fermentations；
(3) deionized water of 1 times of weight is added into above-mentioned material after fermentation, adjusts pH7.0,40 DEG C, digests
2h；PH4.5 is adjusted, 40 DEG C, digests 4h；After the completion of enzymatic hydrolysis, 100 DEG C, 10min is handled, is cooled to room temperature；
(4) dry to moisture content 10% by the material after above-mentioned enzymatic hydrolysis in 55~60 DEG C.
On the basis of above scheme,
The inoculum concentration of the aspergillus oryzae fermenting microbe be 8%, it is described between type neurospora spore suspension inoculum concentration be 7%.
On the basis of above scheme, it is described fermentation algae powder the preparation method comprises the following steps:
(1) bulk kelp is shone into dry grinding, crosses 40 meshes, 121 DEG C of high pressure steam sterilization, 15min are cooled to room temperature, spare；
(2) it is centrifuged the culture solution of chlorella and chrysophyceae respectively, obtains the algal gel of chlorella and chrysophyceae；By chlorella and chrysophyceae
Algal gel mixed according to 1: 1 weight ratio, and the Algemin after accounting for the sterilizing of gross weight 10% is added, is uniformly mixed, it is mixed to obtain algae
Close object；
(3) 1.5~2.0% complex micro organism fungicide is added into algae mixture, 32 DEG C, ferment 24~36h；
(4) after fermentation, into fermentation material be added gross weight 5% mannitol, after mixing, vacuum freeze drying at
Powder is to get fermentation algae powder.
On the basis of above scheme, the complex micro organism fungicide is bacillus subtilis, Lactobacillus rhamnosus by 1:2
Mass ratio composition；
On the basis of above scheme, the preparation method of the laying poultry composite fermented feed additive, step is such as
Under:
(1) it is uniformly mixed by measurement fermentation sunflower seed dregs, ostreae testa pulverata and yellow meal worm powder, Steam explosion treatment；
(2) by the material after above-mentioned Steam explosion treatment successively with fermentation algae powder, isoflavones, carboxymethyl chitosan and
Perilla herb oil be uniformly mixed to get.
On the basis of above scheme, the steam blasting is steam blasting 160s under the conditions of 2.0MPa.
The laying poultry composite fermented feed additive of above method preparation is improving poultry egg laying performance, is improving and lay eggs
Application in performance.
A kind of feed for improving poultry egg laying performance, improving egg laying performance is to add the above method in feed to prepare
Composite fermented feed additive, additive amount are weight fraction 0.2%.
Yellow meal worm powder is purchased from hundred million Jia Kang biotechnology Group Co., Ltd of Qingdao；
Ostreae testa pulverata is purchased from the honest Biotechnology Co., Ltd in Shandong richness field.
The advantages of technical solution of the present invention:
Laying poultry composite fermented feed additive of the invention can not only improve the laying rate of poultry, and increase is laid eggs
Performance, moreover it is possible to further improve quality of laying eggs；Composite fermented feed additive of the invention is by adjusting in the enteron aisle of poultry
Environment promotes the growth and breeding of probiotics, and then promotes the digestion and absorption of nutriment, improves immunity.
Fermentation sunflower seed dregs in laying poultry composite fermented feed additive of the invention contain a large amount of linoleic acid
And linolenic acid, lysine content is high, additionally contains the shell of a large amount of cellulosic, by a hair for type neurospora and aspergillus oryzae
Ferment, can degrade its be difficult to by body digest cellulosic ingredient while, moreover it is possible to macro-molecular protein therein is divided
Solution forms the small-molecular peptides for being easy to be absorbed by organisms, in addition, the fermentation process of type neurospora and aspergillus oryzae can also generate largely
Secondary metabolites and new bioactive ingredients.
Laying poultry of the invention is by chlorella, chrysophyceae and bulk kelp with the fermentation algae powder in composite fermented feed additive
Fermentation material, chlorella and chrysophyceae belong to microalgae, itself contain a large amount of vitamin, microelement, protein, algal polysaccharides
Isoreactivity ingredient, chrysophyceae also contain more carotenoid composition, can be improved immunity of organisms；Bulk kelp cellulosic, pectic substance
With algal polysaccharides rich content, that cellulosic can be promoted to resolve into is solvable after bacillus subtilis and Lactobacillus rhamnosus fermentation
Property fiber, enriches its nutritional ingredient, reduces the content of anti-nutritional factors；In addition, bacillus subtilis can consume in enteron aisle
Oxygen provides anaerobic environment, promotes beneficial bacterium growth；Lactobacillus rhamnosus can promote absorption of the body to calcium phosphorus, it ferments
Lactic acid is generated, the content of organic acid in feed is not only improved, moreover it is possible to improve intestinal environment, inhibit harmful microbe breeding.It is withered
Viable count increases after careless bacillus and Lactobacillus rhamnosus fermentation, can not only increase the nutritive value of algae powder, moreover it is possible to as
A kind of probiotics, adjustment microbial population of animal intestinal tract balance, promote the digestion and absorption of nutriment.
The nutrition of fermentation sunflower seed dregs and both fermentation algae powder in composite fermented feed additive of the present invention complements one another,
Ostreae testa pulverata can provide calcium, P elements, and yellow meal worm powder also contains chitin, antibacterial peptide and defence in addition to providing protein component
Element, isoflavones can adjust the secretion of estrogen, delay senescence；Carboxymethyl chitosan can increase the nutriment of body
Metabolic process improves the antioxygenic property of body, and perilla herb oil is a kind of natural oil of high degree of unsaturation, and contained main ingredient is
Alpha-linolenic acid, content are up to 50%-70%, and alpha-linolenic acid is DHA synthesis material, and the addition of perilla herb oil can increase the battalion laid eggs
Component content is supported, quality of laying eggs is improved.Above-mentioned each component cooperates, and complements one another, and is provided commonly for improving the egg productivity of poultry
It can be with quality of laying eggs.
Specific embodiment
Term as used in the present invention generally has those of ordinary skill in the art usual unless otherwise specified
The meaning of understanding.
Combined with specific embodiments below, and referring to the data further detailed description present invention.Following embodiment only be
It illustrates the present invention, rather than limits the scope of the invention in any way.
Embodiment 1
A kind of preparation method for the algae powder that ferments, steps are as follows:
(1) bulk kelp is shone into dry grinding, crosses 40 meshes, 121 DEG C of high pressure steam sterilization, 15min are cooled to room temperature, spare；
(2) it is centrifuged the culture solution of chlorella and chrysophyceae respectively, obtains the algal gel of chlorella and chrysophyceae；By chlorella and chrysophyceae
Algal gel mixed according to 1: 1 weight ratio, and the Algemin after accounting for the sterilizing of gross weight 10% is added, is uniformly mixed, it is mixed to obtain algae
Close object；
(3) 1.8% complex micro organism fungicide is added into algae mixture, 32 DEG C, ferment 24~36h；
(4) after fermentation, into fermentation material be added gross weight 5% mannitol, after mixing, vacuum freeze drying at
Powder is to get fermentation algae powder.
The complex micro organism fungicide is bacillus subtilis, Lactobacillus rhamnosus is formed by the mass ratio of 1:2.
Bacillus subtilis Bacillus subtilis ACCC 11025, Chinese agriculture Microbiological Culture Collection management in
The heart；
Activation and fermentation medium: peptone 0.5-3g, glucose 1-3g, KH2PO40.02-0.08g、MgSO40.01-
0.04g, NaCl 0.2-0.4g, distilled water 100mL.
11025 strain of bacillus subtilis ACCC is inoculated on solid plate and is activated, the strain after activation is with 1%
Inoculum concentration is inoculated in liquid fermentation medium, and 37 DEG C, 200rpm cultivates 24~36h, until bacterial content >=109cfu/mL。
Lactobacillus rhamnosus is Lactobacillus rhamnosus (Lactobacillus rhamnosus) W27, and deposit number is
CGMCC NO:11387。
Activation and fermentation medium: MRS culture medium；
Lactobacillus rhamnosus W27 strain is inoculated on solid plate and is activated, the strain after activation is connect with 1% inoculum concentration
Kind is in liquid fermentation medium, and 37 DEG C, 200rpm cultivates 24~36h, until bacterial content >=109cfu/mL。
Embodiment 2
Aspergillus oryzae and the activation of type neurospora and the preparation of fermenting microbe
Aspergillus oryzae BNCC195382, Bei Na Chuan Lian Bioisystech Co., Ltd；
Between type neurospora BNCC149795, Bei Na Chuan Lian Bioisystech Co., Ltd；；
Aspergillus oryzae
It crushes beans shell and crosses 10 meshes, sterilize through 121 DEG C, 15min；Water content 50~65% is adjusted, will be trained through fermented bean drink inclined-plane
Aspergillus oryzae kind after supporting base activation is inoculated in peanut shell culture medium, 25-30 DEG C of 48~60h of culture, system by 1% inoculum concentration respectively
Obtain fermenting microbe, strain content >=109cfu·g-1。
Between type neurospora
Activation and fermentation medium: PDA culture medium；
By type neurospora spore between on slant medium under sterile washing, it is prepared into spore suspension, spore suspension is dense
Degree is 107A/mL；It is spare.
Embodiment 3
A kind of preparation method for the sunflower seed dregs that ferment, steps are as follows:
(1) it after sunflower seed dregs remove sundries, is mechanically pulverized, crosses 20 meshes, 121 DEG C of 30min of high pressure steam sterilization have sterilized
Cheng Hou is cooled to room temperature；
(2) adjust sterilizing after sunflower seed dregs moisture content be 55~65%, be inoculated with into peanut meal 8% aspergillus oryzae and
The spore suspension of 7% type neurospora after being sufficiently mixed, carries out solid state fermentation, and 28 DEG C~32 DEG C, after the 36h that ferments, then at 32~
35 DEG C of fermentations are for 24 hours；
(3) deionized water of 1 times of weight is added into above-mentioned material after fermentation, adjusts pH7.0,40 DEG C, digests
2h；PH4.5 is adjusted, 40 DEG C, digests 4h；After the completion of enzymatic hydrolysis, 100 DEG C, 10min is handled, is cooled to room temperature；
(4) dry to moisture content 10% by the material after above-mentioned enzymatic hydrolysis in 55~60 DEG C.
Embodiment 4
A kind of laying poultry composite fermented feed additive is prepared in parts by weight by following components:
Ferment 23 parts of sunflower seed dregs, fermentation 15 parts of algae powder, 2 parts of ostreae testa pulverata, 1 part of yellow meal worm powder, 0.05 part of isoflavones,
0.1 part of carboxymethyl chitosan, 0.05 part of perilla herb oil.
The preparation method of the laying poultry composite fermented feed additive, steps are as follows:
(1) it is uniformly mixed by measurement fermentation sunflower seed dregs, ostreae testa pulverata and yellow meal worm powder, steam blasting under the conditions of 2.0MPa
160s；
(2) by the material after above-mentioned Steam explosion treatment successively with fermentation algae powder, isoflavones, carboxymethyl chitosan and
Perilla herb oil be uniformly mixed to get.
Embodiment 5
A kind of laying poultry composite fermented feed additive is prepared in parts by weight by following components:
Ferment 22 parts of sunflower seed dregs, fermentation 18 parts of algae powder, 3 parts of ostreae testa pulverata, 2 parts of yellow meal worm powder, 0.06 part of isoflavones,
0.18 part of carboxymethyl chitosan, 0.06 part of perilla herb oil.
Preparation method is the same as embodiment 4.
Embodiment 6
A kind of laying poultry composite fermented feed additive is prepared in parts by weight by following components:
Ferment 25 parts of sunflower seed dregs, fermentation 20 parts of algae powder, 4 parts of ostreae testa pulverata, 3 parts of yellow meal worm powder, 0.1 part of isoflavones,
0.3 part of carboxymethyl chitosan, 0.1 part of perilla herb oil.
Preparation method is the same as embodiment 4.
Comparative example 1
A kind of laying poultry composite fermented feed additive is prepared in parts by weight by following components:
Digest 22 parts of sunflower seed dregs, fermentation 18 parts of algae powder, 3 parts of ostreae testa pulverata, 2 parts of yellow meal worm powder, 0.06 part of isoflavones,
0.18 part of carboxymethyl chitosan, 0.06 part of perilla herb oil.
Preparation method is the same as embodiment 4.
The wherein processing method of unleavened sunflower seed dregs are as follows: after sunflower seed dregs remove sundries, be mechanically pulverized, cross 40 mesh
Sieve, 121 DEG C of 30min of high pressure steam sterilization after the completion of sterilizing, are cooled to room temperature；The sterile water of 1 times of amount volume is added, mixing is equal
It is even, it is added 1% alkali protease (Ningxia Sunson Industrial Group Co., Ltd., enzyme activity 200000U/g), adjusts temperature 40~55
DEG C, pH7.5~9, the reaction time is 30~240min, then heats to 85~90 DEG C, keeps 15~20min；0.5% acid is added
Property protease (Ningxia Sunson Industrial Group Co., Ltd., enzyme activity 10000U/g), adjust 45~65 DEG C of temperature, pH3.5~7, instead
It is 30~240min between seasonable, then heats to 85~90 DEG C, keeps 15~20min, enzyme deactivation.By the material after above-mentioned enzymatic hydrolysis in
It is 55~60 DEG C, dry to moisture content 10%.
Comparative example 2
A kind of laying poultry composite fermented feed additive is prepared in parts by weight by following components:
Ferment 22 parts of sunflower seed dregs, fermentation 18 parts of algae powder, 3 parts of ostreae testa pulverata, 2 parts of yellow meal worm powder, 0.06 part of isoflavones,
0.18 part of carboxymethyl chitosan.
Preparation method is the same as embodiment 4.
Comparative example 3
A kind of laying poultry composite fermented feed additive is prepared in parts by weight by following components:
Ferment 22 parts of sunflower seed dregs, fermentation 18 parts of algae powder, 3 parts of ostreae testa pulverata, 2 parts of yellow meal worm powder, 0.06 part of isoflavones,
0.06 part of perilla herb oil.
Preparation method is the same as embodiment 4.
Comparative example 4
A kind of laying poultry composite fermented feed additive is prepared in parts by weight by following components:
Ferment 22 parts of sunflower seed dregs, fermentation 18 parts of algae powder, 3 parts of ostreae testa pulverata, 2 parts of yellow meal worm powder, 0.06 part of isoflavones,
0.18 part of carboxymethyl chitosan, 0.06 part of perilla herb oil.
The preparation method of the laying poultry composite fermented feed additive, steps are as follows:
(1) uniformly mixed by fermentation sunflower seed dregs, ostreae testa pulverata and yellow meal worm powder is measured；
(2) uniformly mixed material is successively mixed with fermentation algae powder, isoflavones, carboxymethyl chitosan and perilla herb oil
Uniformly to get.
One, laying poultry of the present invention composite fermented feed additive effect test
1. test grouping
The laying poultry composite fermented feed additive of embodiment 4~6 and comparative example 1~4
Commercially available laying poultry feed addictive: bodybuilding (fowl is dedicated) is purchased from Shandong Wei Er bioengineering Co., Ltd；
2. test method
Selection health, the sea blue brown shell layer of 190 ages in days 270 similar in weight, are randomly divided into 9 groups, and each group 30
Only；Chicken.Wherein 1~3 group respectively in the basal diet (table 1) addition embodiment 4~6 feed additive, additive amount is
Weight fraction 0.2%；The laying poultry composite fermented feed additive of 4~7 groups of addition comparative examples 1~4 in basal diet,
Additive amount is weight fraction 0.2%；；8th group is added commercially available laying poultry feed addictive in basal diet, and additive amount is attached most importance to
Measure score 0.2%；9th group of basal diet does not add any additive, as blank control group；Using 3 layers of staged cage, often
Its illumination 16h, 5:00~21:00, morning and evening indirect labor's illumination.It is freely eaten and drinks water.Routinely program prevents laying hen
Epidemic disease and coop sterilizing.Daily 6:00 and 15:00 is fed, and 18:00 harvests egg, and other conditions are consistent.Test period 60 days.
1 Basic drawing of table composition and trophic level (air bells dry basis)
| Raw material | Content % | Trophic level | Content % |
| Corn | 69.80 | Thick protein | 16.00 |
| Dregs of beans | 14.45 | Calcium | 3.44 |
| Mountain flour | 8.50 | Phosphorus | 0.59 |
| Peru fish meal | 4.96 | Using phosphorus | 0.42 |
| Calcium monohydrogen phosphate | 1.00 | Salt | 0.33 |
| Premix | 1.00 | Lysine | 0.83 |
| Salt | 0.20 | Methionine | 0.38 |
| Methionine | 0.09 | Methionine+cysteine | 0.63 |
| It is total | 100.00 | Metabolic energy | 11.51 |
Premix provides for every kilogram of complete diet pellet: Mn 60.00mg, Se 0.30mg, Cu 8.00mg, Fe 60.00mg, I
0.35mg, Zn 80.00mg, vitamin A 8000IU, vitamin D 1600IU, vitamin E 5IU, vitamin K 0.50mg, dimension
Raw element B120.004mg, linoleic acid 1.00%, pantothenic acid 2.20mg, thiamine 0.80mg, Riboflavin Tetrabutyrate .50mg, niacin 20.00mg,
Pyridoxol 3.00mg, biotin 0.10mg, folic acid 0.25mg, choline 500.00mg.
3. index determining
The egg number and egg size of every day entry each group chicken calculate laying rate.Every 10d counts 1 feed intake, calculates average day
Feed intake.It is each at the 45th day to repeat to choose 4 pieces of eggs, measure egg Egg Quality.
3.1 production performance indexs
Average daily gain=total feed intake/test number of days；
Feedstuff-egg ratio=feed intake/egg size；
Laying rate=total the number of elements of practical egg number/chicken.
3.2 egg quality
Hangh unit, yolk color pass through multi-functional egg product analysis-e/or determining；Eggshell strength is measured using eggshell strength meter；
Shell thickness is measured using shell thickness meter.
Egg shape index=egg indulges diameter/egg transverse diameter.
3.3 sero-immunity indexs
First day 8:00 after the end of the test, as unit of repetition, each repetition randomly selects 10 chickens, and vein is adopted under wing
Blood collects 5mL with solidifying pipe is promoted, and after standing 30min, is centrifuged 10min, 0.5~1.0mL of Aspirate supernatant, injection in 3000r/min
In 1.5mL centrifuge tube, group, date are marked, -20 DEG C of freezen protective test serum immune indexes are placed in.
3.4 apparent digestibility
4 days before off-test, 3 repetitions of every group of random selection carry out metabolic test using endogenous indicator method, continuous to receive
Collect 4 days excrement, adds 10% hydrochloric acid 10mL by every fresh excrement of 100g after mixing is repeated, to avoid the ammonia nitrogen loss in excrement, excrement sample exists
65% baking oven dries to constant weight, and pulverizer saves to be measured after crushing.Measure dry matter in feed and excrement sample, organic matter, crude protein, total
The content of energy, Ca, P.
It is calculated according to following formula:
Certain Nutrient Apparent Digestibility=100 × [100- (AIA content in AIA content/excrement in feed) × (certain nutrient in excrement
Certain nutrient content in content/feed)].
The measurement of 3.5 cecum microorganisms
On the day before off-test, every group randomly selects 3 chickens, cuts open and kills rear cecal ligation both ends, is put into centrifuge tube plus gives birth to
Salt water gradient dilution is managed, using the quantity of Escherichia coli, Bacillus acidi lactici, Bifidobacterium in spread plate measurement caecum.With this
Bacterial population contained by every gram of cecal content is calculated, as unit of lgCFU/g.
4. test result
Influence of the 4.1 laying poultry composite fermented feed additives of the present invention to egg laying performance
Influence of the laying poultry composite fermented feed additive of the present invention of table 2 to egg laying performance
As shown in Table 2, the average daily gain of embodiment 4~6 and other each groups are not much different, the production of embodiment 4~6
Egg rate and egg size are significantly higher than 1~4 group of comparative example, commercially available additive group and blank control group, the feedstuff-egg ratio of embodiment 4~6
1~4 group of comparative example, commercially available additive group and blank control group, eggshell relative mass embodiment 4~6 are substantially less than with rate of broken eggs
Have the tendency that increasing, but difference is little.It can be seen that laying poultry of the present invention can be mentioned significantly with composite fermented feed additive
The egg laying performance of high poultry.
Influence of the 4.2 laying poultry composite fermented feed additives of the present invention to quality of laying eggs
Influence of the laying poultry composite fermented feed additive of the present invention of table 3 to quality of laying eggs
As shown in Table 3, the egg shape index of embodiment 4~6 is not much different with other each groups, the shell thickness of embodiment 4~6
Slightly above 1~4 group of comparative example, commercially available additive group and blank control group, eggshell strength, yolk color, the egg of embodiment 4~6
Yellow index and hangh unit are significantly higher than 1~4 group of comparative example, commercially available additive group and blank control group, it follows that addition is originally
The nutritional ingredient laid eggs can be improved with composite fermented feed additive for invention laying poultry, improve quality of laying eggs.
Influence of the 4.3 laying poultry composite fermented feed additives of the present invention to Laying-hen Serum immune indexes
Influence of 4 additive of the present invention of table to Laying-hen Serum immune indexes
As shown in Table 4, there was no significant difference for each group serum IgA content, and the IgM content of embodiment 4~6 is significantly higher than comparison
1~4 group of example, commercially available additive group and blank control group, the content of the IgG of embodiment 4~6 be substantially less than 1~4 group of comparative example,
Commercially available additive group and blank control group, it follows that composite fermented feed additive of the invention can by adjust IgM and
The content of IgG adjusts the immunity of poultry.
Influence of the 4.4 laying poultry composite fermented feed additives of the present invention to laying hen apparent digestibility
Influence (%) of the laying poultry composite fermented feed additive of the present invention of table 5 to laying hen apparent digestibility
As shown in Table 5, the dry matter, energy, crude protein, organic matter of embodiment 4~6, Ca and P digestibility are significantly higher than pair
1~4 group of ratio, commercially available additive group and blank control group, it follows that laying poultry of the present invention is added with fermented composite feed
Agent can significantly improve the apparent digestibility of laying hen.
Influence of the 4.5 laying poultry composite fermented feed additives of the present invention to laying hen cecum microorganisms
Influence (lgcfu/g) of the laying poultry composite fermented feed additive of the present invention of table 6 to laying hen cecum microorganisms
As shown in Table 6, in 4~6 groups of embodiment of laying hen caecum the quantity of Escherichia coli be substantially less than 1~4 group of comparative example,
The quantity of commercially available additive group and blank control group, Bifidobacterium and Bacillus acidi lactici is significantly higher than 1~4 group of comparative example, commercially available adds
Add agent group and blank control group, this illustrates that composite fermented feed additive of the invention can dramatically increase caecum beneficial microbe
Growth and breeding, inhibit harmful microbe growth, ambient stable in poultry intestinal tract maintained with this, the substance that has additional nutrients disappears
Change and absorbs.
The above embodiments are merely illustrative of the technical solutions of the present invention, rather than is limited；Although referring to aforementioned reality
Applying example, invention is explained in detail, for those of ordinary skill in the art, still can be to aforementioned implementation
Technical solution documented by example is modified or equivalent replacement of some of the technical features；And these are modified or replace
It changes, the spirit and scope for claimed technical solution of the invention that it does not separate the essence of the corresponding technical solution.
Claims (10)
1. a kind of laying poultry composite fermented feed additive, it is characterised in that: prepared in parts by weight by following components and
At:
22~25 parts of sunflower seed dregs of fermentation, 15~20 parts of algae powder of fermentation, 2~4 parts of ostreae testa pulverata, 1~3 part of yellow meal worm powder, soybean are different
0.05~0.1 part of flavones, 0.1~0.3 part of carboxymethyl chitosan, 0.05~0.1 part of perilla herb oil.
2. laying poultry composite fermented feed additive according to claim 1, it is characterised in that: by following components by weight
Amount number is prepared:
Ferment 22 parts of sunflower seed dregs, fermentation 18 parts of algae powder, 3 parts of ostreae testa pulverata, 2 parts of yellow meal worm powder, 0.06 part of isoflavones, carboxylic first
0.18 part of base enclosure glycan, 0.06 part of perilla herb oil.
3. laying poultry composite fermented feed additive according to claim 1 or claim 2, it is characterised in that: the fermentation certain herbaceous plants with big flowers
Flower the seed dregs of rice the preparation method comprises the following steps:
(1) it after sunflower seed dregs remove sundries, is mechanically pulverized, crosses 20 meshes, 121 DEG C of 30min of high pressure steam sterilization, sterilizing is completed
Afterwards, it is cooled to room temperature；
(2) moisture content for adjusting sunflower seed dregs after sterilizing is 55~65%, and 7~10% aspergillus oryzaes and 5 are inoculated with into peanut meal
The spore suspension of~8% type neurospora, after being sufficiently mixed, carry out solid state fermentation, 28 DEG C~32 DEG C, ferment 36h after, then at 32
~35 DEG C of fermentations are for 24 hours；
(3) deionized water of 1 times of weight is added into above-mentioned material after fermentation, adjusts pH7.0,40 DEG C, digests 2h；It adjusts
PH4.5 is saved, 40 DEG C, digests 4h；After the completion of enzymatic hydrolysis, 100 DEG C, 10min is handled, is cooled to room temperature；
(4) dry to moisture content 10% by the material after above-mentioned enzymatic hydrolysis in 55~60 DEG C.
4. laying poultry composite fermented feed additive according to claim 3, it is characterised in that:
The inoculum concentration of the aspergillus oryzae fermenting microbe be 8%, it is described between type neurospora spore suspension inoculum concentration be 7%.
5. laying poultry composite fermented feed additive according to claim 1 or claim 2, it is characterised in that: the fermentation algae
Powder the preparation method comprises the following steps:
(1) bulk kelp is shone into dry grinding, crosses 40 meshes, 121 DEG C of high pressure steam sterilization, 15min are cooled to room temperature, spare；
(2) it is centrifuged the culture solution of chlorella and chrysophyceae respectively, obtains the algal gel of chlorella and chrysophyceae；By the algae of chlorella and chrysophyceae
Mud is mixed according to 1: 1 weight ratio, and the Algemin after the sterilizing for accounting for gross weight 10% is added, and is uniformly mixed, is obtained algae mixture；
(3) 1.5~2.0% complex micro organism fungicide is added into algae mixture, 32 DEG C, ferment 24~36h；
(4) after fermentation, the mannitol of gross weight 5% is added into fermentation material, after mixing, vacuum freeze drying is at powder
End is to get fermentation algae powder.
6. laying poultry composite fermented feed additive according to claim 5, it is characterised in that: the complex microorganism
Microbial inoculum is bacillus subtilis, Lactobacillus rhamnosus is formed by the mass ratio of 1:2.
7. the preparation method of any one of the claim 1~6 laying poultry composite fermented feed additive, feature exist
In: steps are as follows:
(1) it is uniformly mixed by measurement fermentation sunflower seed dregs, ostreae testa pulverata and yellow meal worm powder, Steam explosion treatment；
(2) by the material after above-mentioned Steam explosion treatment successively with fermentation algae powder, isoflavones, carboxymethyl chitosan and purple perilla
Oil be uniformly mixed to get.
8. the preparation method of laying poultry composite fermented feed additive according to claim 7, it is characterised in that: described
Steam blasting is steam blasting 160s under the conditions of 2.0MPa.
9. claim 8 the method preparation laying poultry composite fermented feed additive improve poultry egg laying performance,
Improve the application in quality of laying eggs.
10. a kind of feed for improving poultry egg laying performance, improving quality of laying eggs, it is characterised in that: add right in feed and want
The composite fermented feed additive for asking 8 the methods to prepare, additive amount are weight fraction 0.2%.
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