CN109283281A - A kind of chromatographic column - Google Patents

A kind of chromatographic column Download PDF

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Publication number
CN109283281A
CN109283281A CN201811198035.5A CN201811198035A CN109283281A CN 109283281 A CN109283281 A CN 109283281A CN 201811198035 A CN201811198035 A CN 201811198035A CN 109283281 A CN109283281 A CN 109283281A
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China
Prior art keywords
chromatotube
chromatographic column
damped part
analysans
air inlet
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CN201811198035.5A
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Chinese (zh)
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CN109283281B (en
Inventor
雷晓强
鲁军虎
石地娟
高艳华
魏伟
俄晓
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Lanzhou Dongli Dragon Information Technology Co Ltd
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Lanzhou Dongli Dragon Information Technology Co Ltd
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    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N30/00Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
    • G01N30/02Column chromatography
    • G01N30/60Construction of the column
    • G01N30/6052Construction of the column body
    • G01N30/606Construction of the column body with fluid access or exit ports

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  • Physics & Mathematics (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Analytical Chemistry (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • General Physics & Mathematics (AREA)
  • Immunology (AREA)
  • Pathology (AREA)
  • Treatment Of Liquids With Adsorbents In General (AREA)
  • Sampling And Sample Adjustment (AREA)

Abstract

The present invention provides a kind of chromatographic column, and including damped part and hollow chromatotube, the overall diameter of damped part is less than the interior diameter of chromatotube, and damped part is set in the hollow pipeline of chromatotube.Compared with prior art, chromatographic column provided by the invention, solution is mostly the chromatographic column of certain specification in the prior art, accurate chromatography cannot be carried out by the analysans to small molecule structure well, and the problem of existing chromatographic column is to the analysis result badly of the analysans of small molecule structure, therefore, chromatographic column provided by the invention, by the way that damped part is arranged, so that speed when analysans passes through chromatographic column slows down, accurate chromatography is carried out so as to the analysans to small molecule structure.

Description

A kind of chromatographic column
Technical field
The present invention relates to chromatography field more particularly to a kind of chromatographic columns.
Background technique
Chromatography is a kind of separation analysis means, and separation is core, therefore the chromatographic column for undertaking centrifugation is chromatographic system Heart.Chromatographic column is made of column tube, pressure cap, cutting ferrule (sealing ring), sieve plate (filter disc), connector, screw etc..Column tube is mostly used stainless Steel is made, and heavy-walled glass or quartz ampoule can also be used, and inside pipe wall requires have very high finish.When due to chromatography, not only The analysans of macromolecular structure can be analyzed, can also analyze the analysans of small molecule structure, macromolecular structure and small point For minor structure due to sole mass difference, the speed Jing Guo chromatographic column is also different.And currently there are chromatographic column Mostly it is the chromatographic column of certain specification, accurate chromatography cannot be carried out by the analysans to small molecule structure very well, thus Analysis result badly of the existing chromatographic column to the analysans of small molecule structure.Therefore it provides a kind of chromatographic column, it can be to small The analysans of molecular structure carries out accurate chromatography, is that existing market needs.
Summary of the invention
The main technical problem to be solved in the present invention, which is to provide one kind, can carry out essence to the analysans of small molecule structure The chromatographic column of true chromatography, cannot be well to small molecule structure to solve the chromatographic column of certain specification in the prior art Analysans carries out the problem of accurate chromatography and analysis result badly.
In order to solve the above technical problems, the present invention provides a kind of chromatographic column, including damped part and hollow chromatotube, resistance The overall diameter of Buddhist nun's component is less than the interior diameter of chromatotube, and damped part is set in the hollow pipeline of chromatotube.
Further, damped part is less than the length that chromatotube extends along its length along the length of length extending direction.
Further, damped part includes upper port, lower port;Chromatotube includes air inlet, gas outlet.
Upper port is concordant with air inlet;Or
Lower port is concordant with gas outlet;Or
Upper port is vertically lower than air inlet and lower port is positioned vertically higher than gas outlet.
Further, sealing element is provided at upper port and lower port.
Further, damped part is connected in the hollow pipeline of chromatographic column by laser welding.
Further, damped part includes damping column or hollow damper tube.
Further, the interior diameter of the overall diameter of damping column or damper tube reduces or increases along the direction far from air inlet Greatly.
Further, the outer wall of damping column or the inner wall of damper tube are provided with lines.
Further, drying device is equipped at air inlet.
Further, drying device includes dry adsorption layer, and dry adsorption layer is arranged in the hollow pipeline of chromatotube On wall.
The beneficial effects of the present invention are:
The present invention provides a kind of chromatographic column, and including damped part and hollow chromatotube, the overall diameter of damped part is less than The interior diameter of chromatotube, damped part are set in the hollow pipeline of chromatotube.Compared with prior art, color provided by the invention Column is composed, the chromatographic column of certain specification in the prior art is solved, the analysans to small molecule structure cannot be carried out accurately well Chromatography and analysis result badly the problem of.Therefore, chromatographic column provided by the invention, by the way that damped part is arranged, so that Speed when analysans passes through chromatographic column slows down, and carries out accurate chromatography point so as to the analysans to small molecule structure Analysis.
Detailed description of the invention
It, below will be to use required in embodiment in order to illustrate more clearly of the technical solution of embodiment of the present invention Attached drawing be briefly described, it should be understood that the following drawings illustrates only certain embodiments of the present invention, therefore is not to be seen as It is the restriction to range, it for those of ordinary skill in the art, without creative efforts, can be with root Other relevant attached drawings are obtained according to these attached drawings.
Fig. 1 is that a kind of structure of the chromatographic column of the first way provided in an embodiment of the present invention for being provided with damped part is shown It is intended to;
Fig. 2 is that a kind of structure of the chromatographic column of the second way provided in an embodiment of the present invention for being provided with damped part is shown It is intended to;
Fig. 3 is the structural schematic diagram of the chromatographic column provided in an embodiment of the present invention for being provided with another damped part.
Appended drawing reference:
1, chromatotube;2, damped part;10, drying device;11, air inlet;12, gas outlet;13, hollow pipeline;21, on Port;22, lower port;23, lines.
Specific embodiment
In order to make the object, technical scheme and advantages of the embodiment of the invention clearer, below in conjunction with the embodiment of the present invention In attached drawing, technical scheme in the embodiment of the invention is clearly and completely described, it is clear that described embodiment is A part of the embodiment of the present invention, instead of all the embodiments.The present invention being usually described and illustrated herein in the accompanying drawings is implemented The component of example can be arranged and be designed with a variety of different configurations.
Therefore, the detailed description of the embodiment of the present invention provided in the accompanying drawings is not intended to limit below claimed The scope of the present invention, but be merely representative of selected embodiment of the invention.Based on the embodiments of the present invention, this field is common Technical staff's every other embodiment obtained without creative efforts belongs to the model that the present invention protects It encloses.
It should also be noted that similar label and letter indicate similar terms in following attached drawing, therefore, once a certain Xiang Yi It is defined in a attached drawing, does not then need that it is further defined and explained in subsequent attached drawing.
In the description of the present invention, it should be noted that the orientation of the instructions such as term " on ", "inner", "bottom" or position are closed System for be based on the orientation or positional relationship shown in the drawings or the invention product using when the orientation usually put or position close System, is merely for convenience of description of the present invention and simplification of the description, rather than the device or element of indication or suggestion meaning must have Specific orientation is constructed and operated in a specific orientation, therefore is not considered as limiting the invention, in addition, term " the One ", " second " etc. is only used for distinguishing description, is not understood to indicate or imply relative importance.
In the description of the present invention, it is also necessary to which explanation is unless specifically defined or limited otherwise, term " setting ", " connected ", " connection " shall be understood in a broad sense, such as: it may be a fixed connection, may be a detachable connection, or integrally connect It connects;It can be mechanical connection, be also possible to be electrically connected;It can be directly connected, can also indirectly connected through an intermediary, it can To be the connection inside two elements.For the ordinary skill in the art, above-mentioned term can be understood with concrete condition Concrete meaning in the present invention.
Below by specific embodiment combination attached drawing, invention is further described in detail.
To solve the chromatographic column that chromatographic column in the prior art is mostly certain specification, cannot very well to small molecule structure to Analyte carries out accurate chromatography, and have existing chromatographic column to the analysis result of the analysans of small molecule structure not Good problem.A kind of chromatographic column is present embodiments provided, including damped part and hollow chromatotube, the overall diameter of damped part Less than the interior diameter of chromatotube, damped part is set in the hollow pipeline of chromatotube.
Specifically, referring to Figure 1, Fig. 2, Fig. 3, chromatographic column provided in this embodiment includes chromatotube 1 and damped part 2. When analyzing the analysans of small molecule structure, due to the analysans lighter weight of small molecule structure, passing through chromatotube 1 When, it is bad to eventually lead to chromatography effect so that analysis is not exclusively for excessive velocities.In chromatographic column provided in this embodiment It is provided with damped part 2, speed of the analysans of small molecule structure by chromatotube 1 when can be slowed down, so that small molecule structure Analysans chromatography result it is more accurate.
Further, referring to Figure 1, Fig. 2, Fig. 3, chromatotube 1 include air inlet 11, gas outlet 12, hollow pipeline 13 Both ends are connect with air inlet 11 and gas outlet 12 respectively.Damped part 2 includes upper port 21, lower port 22, and damped part 2 is set to In the hollow pipeline 13 of chromatographic column.Specifically, damped part 2 can also can be connected by laser by welding Mode is set in the hollow pipeline 13 of chromatotube 1, and specific fixing mode can be not limited to the mode that the present embodiment provides, As long as realizing that damped part 2 can be set in chromatotube 1, the present embodiment is not especially limited this.
It is to be appreciated that being so that damped part 2 is set in chromatotube 1, by reducing setting in 1 internal resistance of chromatotube The mode of the outer diameter of Buddhist nun's component 2 reduces the cross section that the analysans of small molecule structure flows through chromatotube 1, reduces small molecule knot The analysans of structure flows through the flow of chromatotube 1, so that the speed that analysans flows through chromatotube 1 is reduced, so that small point The chromatography result of the analysans of minor structure is more accurate.In order to preferably reach such effect, damped part 2 it is outer Diameter needs to be less than the internal diameter of the hollow pipeline 13 of chromatotube 1.Specifically, in the size of the outer diameter of damped part 2, chromatotube 1 The size and the difference between them of 13 internal diameter of vacant duct, depending on the circumstances, the present embodiment does not limit this specifically It is fixed.
Further, damped part 2 is less than chromatotube 1 along the length of length extending direction along the length of length extending direction Degree.Since damped part 2 is to be arranged in chromatotube 1, thus damped part 2 flows through chromatography to the analysans of small molecule structure The speed of pipe 1 plays the role of one and slows down.
Specifically, as shown in Figure 1, Figure 2, Figure 3 shows, in the present embodiment, on the length extending direction of chromatotube 1, damping The length of component 2 is less than the length of chromatotube 1, and the outer diameter of damped part 2 needs to be less than the interior of the hollow pipeline 13 of chromatotube 1 Diameter, therefore damped part 2 has the speed for flowing through chromatotube 1 to the analysans of small molecule structure to slow down effect, it is specific to hinder The difference that the length of Buddhist nun's component 2 is less than the length of chromatotube 1 depends on the circumstances, and the present embodiment is not especially limited this.
Further, as shown in Figure 1, Figure 2, Figure 3 shows, the damped part 2 of the present embodiment includes upper port 21, lower port 22, Chromatotube 1 includes air inlet 11, gas outlet 12, and upper port 21 is concordant with air inlet 11, or, lower port 22 and gas outlet 12 are flat Together, or, upper port 21 is vertically lower than air inlet 11, lower port 22 is positioned vertically higher than gas outlet 12.
Specifically, as shown in Figure 1, the length due to damped part 2 along length extending direction should be set smaller than chromatotube 1 Along the length of length extending direction, when the upper port 21 of damped part 2 is concordant with air inlet 11, damped part 2 is arranged in color At the upper section for composing pipe 1, the upper surface of damped part 2 is concordant with the end face of chromatotube 1.As shown in Figure 1, making small molecule structure Analysans can slowly enter in the tubing string of chromatotube 1, then very fast outflow, can obtain better analytical effect.
Specifically, as shown in Fig. 2, damped part 2 is arranged when the lower port 22 of damped part 2 is concordant with gas outlet 12 It is concordant with the end face of chromatotube 1 in the bottom face of the lower section of chromatotube 1, damped part 2, so that small molecule structure is to be analyzed After object can be rapidly introduced into the tubing string of chromatotube 1, then slowly outflow, better analytical effect can be obtained.
In addition, the upper port 21 when damped part 2 is vertically lower than air inlet 11, lower port 22 in vertical direction When being above higher than gas outlet 12, the middle section of chromatotube 1 is arranged in damped part 2 (the present embodiment does not provide schematic diagram).It needs It is bright, no matter damped part 2 be arranged at the upper section of chromatotube 1, lower section or middle section, do not affect damped part 2 Play the role of one to the speed that the analysans of small molecule structure flows through chromatotube 1 to slow down, therefore, can select according to actual needs The position that damped part 2 is arranged in chromatotube 1 is selected, the present embodiment is not especially limited this.
Further, in the present embodiment, sealing element (figure is provided at the upper port 21 and lower port 22 of damped part 2 In be not shown).It, in use, should also be at upper port 21 and lower port 22 to ensure result reliability when chromatography It is provided with sealing element, it is ensured that the airtightness in chromatotube 1.Specifically, sealing element can be gasket seal, it is also possible to other tools There is the component of sealing function, the present embodiment is not especially limited this.
Further, damped part 2 is by laser welding in the hollow pipeline 13 of chromatotube 1.In the prior art, it will hinder In general the mode that Buddhist nun's component 2 is set in chromatotube 1 is by laser welding, naturally it is also possible to be not limited only to this implementation The laser welding mode that example provides, specific connection type depend on the circumstances, and the present embodiment is not especially limited this.
Fig. 2 is referred to, in the present embodiment, drying device 10 is equipped at the air inlet 11 of chromatotube 1, drying device 10 wraps Dry adsorption layer is included, dry adsorption layer is arranged on the tube wall of hollow pipeline 13.To avoid having part dirty in the carrier gas after gasification Dye object is doped into chromatographic column, influences the chromatography result accuracy of analysans, needs to be equipped at air inlet 11 dry Dry device 10.
Specifically, drying device 10 can be dry adsorption layer, dry adsorption layer is arranged on the tube wall of hollow pipeline 13, To carry out a dry decontamination processing to the carrier gas after gasification, the chromatography accuracy of analysans is improved.
Further, damped part 2 includes damper tube or damping column.Fig. 3 is referred to, is that a kind of damped part 2 is in Fig. 3 The structural schematic diagram of damping column.Damped part 2 flows through the speed of chromatotube 1 for reducing the analysans of small molecule structure, makes The analysans for obtaining small molecule structure can preferably carry out chromatography, and result is also more accurate.
Specifically, damped part 2, which can be damper tube, is also possible to damping column, damped part 2 is not affected to small molecule The speed that the analysans of structure flows through chromatotube 1 plays the role of slowing down.Therefore, damped part can be selected according to actual needs 2 structure and type, the present embodiment are not especially limited this.
As shown in Fig. 2, in one embodiment of the invention, damped part 2 is damping column or damper tube.Specifically, resistance The overall diameter of Buddhist nun's column or the interior diameter of damper tube reduce along the direction far from air inlet 11, when carrying out chromatography, when need When wanting analysans with speed first quick and back slow by chromatotube 1, can be set damping column overall diameter or damper tube it is interior straight Diameter reduces along the direction far from air inlet 11, after enabling to analysans to be rapidly introduced into the tubing string of chromatotube 1, then delays Slug flow goes out, so that analytical effect is more preferable.
Further, as shown in Figure 1, in another embodiment of the present invention, damped part 2 is damping column or damping Pipe.Specifically, the overall diameter of damping column or the interior diameter of damper tube increase along the direction far from air inlet 11, chromatography is being carried out When analysis, after needing analysans first slow fastly by chromatotube 1 when, can be set damping column overall diameter or damper tube it is interior Diameter increases along the direction far from air inlet 11, and analysans is enabled to slowly little by little to enter the tubing string of chromatotube 1 In, then very fast outflow, so that analytical effect is more preferable.
As shown in Figure 1, Figure 2, in the present embodiment, 2 inner wall of damped part is provided with lines 23.Damped part 2 is used known to above The speed of chromatotube 1 is flowed through in the analysans for reducing small molecule structure, allows the analysans of small molecule structure more preferable Carry out chromatography, result is also more accurate.When the analysans for needing to further decrease small molecule structure flows through chromatography The speed of pipe 1 can also be provided with lines 23 in 2 inner wall of damped part when obtaining the chromatography result of more pinpoint accuracy, have The lines 23 of body can be frosted lines etc., and the analysans for further slowing down small molecule structure flows through the speed of chromatotube 1, makes It is higher to obtain chromatography accuracy.
The present invention provides a kind of chromatographic column, and including damped part and hollow chromatotube, the overall diameter of damped part is less than The interior diameter of chromatotube, damped part are set in the hollow pipeline of chromatotube.Compared with prior art, color provided by the invention Column is composed, be able to solve the certain chromatographic column of specification in the prior art the analysans to small molecule structure cannot carry out accurately very well Chromatography, analysis result badly the problem of.Therefore, chromatographic column provided by the invention can be made by setting damped part It obtains speed when analysans passes through chromatographic column to slow down, carries out accurate chromatography so as to the analysans to small molecule structure Analysis.
The above content is specific embodiment is combined, further detailed description of the invention, and it cannot be said that this hair Bright specific implementation is only limited to these instructions.For those of ordinary skill in the art to which the present invention belongs, it is not taking off Under the premise of from present inventive concept, a number of simple deductions or replacements can also be made, all shall be regarded as belonging to protection of the invention Range.

Claims (10)

1. a kind of chromatographic column, which is characterized in that including damped part and hollow chromatotube, the overall diameter of the damped part is small In the interior diameter of the chromatotube, the damped part is set in the hollow pipeline of the chromatotube.
2. chromatographic column as described in claim 1, which is characterized in that the damped part is along the length extension side of the chromatotube To length be less than the chromatotube length.
3. chromatographic column as described in claim 1, which is characterized in that the damped part includes upper port, lower port;The color Spectrum pipe includes air inlet, gas outlet;
The upper port is concordant with the air inlet;Or
The lower port is concordant with the gas outlet;Or
On the length extending direction along the chromatotube, the upper port is lower than the air inlet, and the lower port is high In the gas outlet.
4. being provided with sealing element at chromatographic column as claimed in claim 3, the upper port and the lower port.
5. chromatographic column as described in claim 1, which is characterized in that the damped part is by laser welding in the chromatotube The hollow pipeline in.
6. chromatographic column as described in any one in claim 1-5, which is characterized in that the damped part includes damping column or hollow Damper tube.
7. chromatographic column as claimed in claim 6, which is characterized in that the overall diameter of the damping column or the damper tube it is interior straight Diameter is decreased or increased along the direction far from the air inlet.
8. chromatographic column as claimed in claim 6, which is characterized in that the inner wall of the outer wall of the damping column or the damper tube is set It is equipped with lines.
9. chromatographic column as described in claim 3 or 4, which is characterized in that be equipped with drying device at the air inlet.
10. chromatographic column as claimed in claim 9, which is characterized in that the drying device includes dry adsorption layer, the drying Adsorption layer is arranged on the inner wall of the hollow pipeline of the chromatotube.
CN201811198035.5A 2018-10-15 2018-10-15 Chromatographic column Active CN109283281B (en)

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