CN205538682U - Fluorescence flow -through cell of terminal surface photic - Google Patents
Fluorescence flow -through cell of terminal surface photic Download PDFInfo
- Publication number
- CN205538682U CN205538682U CN201620093969.2U CN201620093969U CN205538682U CN 205538682 U CN205538682 U CN 205538682U CN 201620093969 U CN201620093969 U CN 201620093969U CN 205538682 U CN205538682 U CN 205538682U
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- Prior art keywords
- drain pipe
- fluorescence flow
- flow cell
- cell
- fluorescence
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- Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)
- Optical Measuring Cells (AREA)
Abstract
The utility model discloses a fluorescence flow -through cell of terminal surface photic, be in including transparent cell body and setting inside feed liquor pipeline and the drain pipe way of cell body, feed liquor pipeline end extends to the surface of cell body forms the feed liquor end, go out liquid duct extension extremely the surface of cell body forms out the liquid end, the feed liquor pipeline with the drain pipe way becomes V -arrangement setting and intercommunication, and the intercommunication is in another surface of cell body forms and is used for kicking into the incident end that excites the light beam, the terminal surface of incident end with the axle center that the drain pipe was said is perpendicular. The utility model provides a tradition fluorescence flow -through cell problem that laterally the easy scattered light that produces disturbed under photic and side -emitting's the circumstances, this fluorescence flow -through cell advances the liquid outlet and adopts conical surface design simultaneously, can effectively reduce flow path interface dead volume, reduction solution cross contamination.
Description
Technical field
The utility model belongs to fluorescent optics detecting instrument technical field, particularly relates to the fluorescence flow cell of a kind of end face light.
Background technology
Fluorescence detector is detector common on liquid chromatogram instrument, it is possible to be provided on sepectrophotofluorometer instrument.Glimmering
In photodetector, flow cell is all important parts.In fluorescence detector, the exciting light from light source irradiates fluorescence flow cell
In sample solution, light energy is absorbed by sample Middle molecule, and sample molecule is excited and launches wavelength and differ with excitation wavelength
The fluorescence spectrum caused.In order to reduce the excitation source impact on fluorescent spectrum, as described in Figure 5, by glimmering with transmitting for exciting light
Light design has a certain degree, and is commonly designed at a right angle.Meanwhile, fluorescence detector flow cell is also required to be designed to bear necessarily
The device of sample flow pressure, to meet the pressure demand on liquid chromatogram instrument, the resistance to pressure request of usual fluorescence flow cell is 2MPa.
The fluorescence detector sample cell capacity being commonly used on liquid-phase chromatographic analysis is below 10uL, and sample cell internal diameter is all at 1mm
Left and right, in such cases, uses and excites from side sample again from the mode of side detection fluorescence, and exciting light will be by sample cell
Wall is directly reflected in the light path system that fluorescence is launched in detection, produces the faintest sample fluorescence signal greatly
Scattered light interference, affect the detection to week fluorescent material of fluorescence detector, affect detection limit index.
The quartz material fluorescence flow cell being commonly used on liquid-phase chromatographic analysis, its liquid entrance part all uses planar fashion
It is attached with external pipeline, generally uses soft rubber-plastics material, if the PTFE polytetrafluoroethylene (PTFE) commonly used is as seal washer,
By the compressive deformation of seal washer, it is achieved the pressure-resistant seal between quartz interface through hole and external pipeline.This kind of plane and pipeline
Connected mode, easily produce gap at interface, form the dead volume in pipeline, cause solution cross pollution, impact detection
Precision, and higher to installation requirement, tend to produce leakage because of Rig up error.Therefore, needing one at present badly can be effective
Reduce and excite scattered light interference effect, and be not likely to produce the fluorescence flow cell of stream dead volume.
In order to overcome drawbacks described above of the prior art, the utility model proposes the fluorescence flow cell of a kind of end face light, it solves
The problem of the scattered light interference that certainly conventional fluorescent flow cell easily produces in the case of side light and lateral emitting, this is glimmering simultaneously
Light flow cell liquid in-out mouth uses conical surface design, is effectively reduced stream interface dead volume, reduces solution cross pollution.
Utility model content
The utility model proposes the fluorescence flow cell of a kind of end face light, including transparent pond body be arranged on portion in the body of described pond
Input duct and drain pipe road；Described input duct end extends to the surface of described pond body and forms liquid feeding end, described drain pipe road
The surface extending to described pond body forms outlet end；Described input duct arranges with described drain pipe road forming V-shape and connects, connection
Another surface being in described pond body forms the incidence end for injecting excitation beam, the end face of described incidence end and described drain pipe
The axis perpendicular in road.
In the fluorescence flow cell of the described end face light that the utility model proposes, outside described incidence end, closely it is provided with light transmission piece.
In the fluorescence flow cell of the described end face light that the utility model proposes, described liquid feeding end and described outlet end are respectively equipped with recessed
The seal fallen into.
In the fluorescence flow cell of the described end face light that the utility model proposes, described seal becomes cone to cave in.
In the fluorescence flow cell of the described end face light that the utility model proposes, described liquid feeding end and described outlet end further with lead
The liquid seal of tube connects, and the periphery of the end of described catheter is provided with the seal cooperated with described seal.
In the fluorescence flow cell of the described end face light that the utility model proposes, described seal is made up of elastomeric material.
The beneficial effects of the utility model are:
The utility model fluorescence flow cell provides a kind of by end face light, and the fluorescence flow cell detection mode of fluorescence is detected in side,
Exciting light can be prevented effectively from and directly entered the light path system of fluorescence emission by the mirror-reflection at interface, reduce in fluoroscopic examination
Excite scattered light to disturb, improve the accuracy of detection.
The utility model seals in portion in the body of pond, without using other sealing device again outside flow cell end, reduces leakage
Risk, decreases interface dead volume, simultaneously facilitates cleaning, decreases the cross pollution of stream sample, improves the essence of detection
Exactness.
Accompanying drawing explanation
Fig. 1 is the top view of the utility model fluorescence flow cell.
Fig. 2 is the K-K sectional view of the utility model fluorescence flow cell.
Fig. 3 is the K-K sectional view connecting the fluorescence flow cell having catheter.
Fig. 4 is the utility model fluorescence flow cell and exciting light thereof and the stereogram of transmitting fluorescence.
Fig. 5 is flow cell and exciting light thereof and the schematic diagram of transmitting fluorescence in prior art.
Detailed description of the invention
In conjunction with specific examples below and accompanying drawing, the utility model is described in further detail.Implement mistake of the present utility model
Journey, condition, experimental technique etc., outside the lower content mentioned specially, be universal knowledege and the common knowledge of this area,
The utility model is not particularly limited content.
Seeing in Fig. 1 and Fig. 2, this detailed description of the invention, the fluorescence flow cell of this end face light includes transparent pond body 1 and sets
Put in the input duct 2 within pond body 1 and drain pipe road 3.Pond body 1 is to use suprasil body material to make.
Input duct 2 and drain pipe road 3 use cheesing techniques to give a farfetched interpretation and form, and utilize polishing technology to carry out its inner surface of pipeline
Polishing so that the bright and clean degree of tube wall reaches pellucidity.Input duct 2 end extends to the surface of pond body 1 and forms liquid feeding end 21,
Drain pipe road 3 extends to the surface of pond body 1 and forms outlet end 31.Input duct 2 arranges with drain pipe road 3 forming V-shape and connects,
Connectivity part forms the incidence end 4 for injecting excitation beam, the end face of incidence end 4 and drain pipe road 3 on another surface of pond body 1
Axis perpendicular.The outside of incidence end 4 can closely arrange a piece of light transmission piece 5, can be used for making sample liquid suitable from input duct 2
Profit enters drain pipe road 3.
Sample liquid flows in input duct 2 from liquid feeding end 21, flows into drain pipe road 3 by incidence end 4 afterwards internal, finally
Flow out from outlet end 31.When exciting light is injected drain pipe road 3 from the end face of incidence end 4, exciting light illuminates drain pipe road 3
Internal sample liquid and then therefrom launch fluorescence, the fluorescence of transmitting be perpendicular to the axle center in drain pipe road 3 from the side of pond body 1 to
External emission, arranging measurement apparatus at its transmitting terminal can detect this transmitting fluorescence.
In preferred embodiment of the present utility model, liquid feeding end 21 and outlet end 31 are respectively equipped with the seal of depression, it is to avoid
Carry out outside pond body 1 surface sealing the unnecessary dead volume caused.The 3D shape of this seal can be semicircular body, cuboid etc.
The Arbitrary 3 D structure that various those skilled in the art are readily apparent that, in this preferred embodiment, the 3D shape of this seal is inside
Depression and the cone shunk.
Seal to realize perfection with above-mentioned seal, thus corresponding seal 7 is set in the periphery of the end of catheter 6.
In the utility model preferred embodiment as shown in Figure 3, this seal 7 is made up of PTFE polytetrafluoroethylene (PTFE) elastomeric material,
A circular hole equal with the external diameter of catheter 6 is dug by central authorities at elastomeric material, is penetrated in circular hole by catheter 6 thus makes
It is provided with the catheter of seal 7, owing to catheter 6 is made up of hard material, its end and input duct 2 and/or drain pipe road
3 docking, deform by catheter 6 applies pressure making seal 7 be squeezed in seal, thus realize catheter 6
With the perfect sealing in input duct 2 and/or drain pipe road 3, and decrease and be originally positioned at the dead volume outside pond body 1.This sealing
The 3D shape of part 7 can cooperate with the 3D shape of seal, and the such as 3D shape of this seal 7 is and seal
Corresponding cone.
Protection content of the present utility model is not limited to above example.Under the spirit and scope conceived without departing substantially from utility model,
Those skilled in the art it is conceivable that change and advantage be all included in the utility model, and with appending claims
For protection domain.
Claims (6)
1. the fluorescence flow cell of an end face light, it is characterised in that include transparent pond body (1) and be arranged on the internal input duct (2) in described pond body (1) and drain pipe road (3)；Described input duct (2) end extends to the surface in described pond body (1) and forms liquid feeding end (21), and described drain pipe road (3) extends to the surface in described pond body (1) and forms outlet end (31)；Described input duct (2) arranges with described drain pipe road (3) forming V-shape and connects, connectivity part forms the incidence end (4) for injecting excitation beam, the end face of described incidence end (4) and the axis perpendicular of described drain pipe road (3) on another surface in described pond body (1).
2. the fluorescence flow cell of end face light as claimed in claim 1, it is characterised in that described incidence end (4) is the most closely provided with light transmission piece (5).
3. the fluorescence flow cell of end face light as claimed in claim 1, it is characterised in that described liquid feeding end (21) and described outlet end (31) are respectively equipped with the seal of depression.
4. the fluorescence flow cell of end face light as claimed in claim 3, it is characterised in that described seal becomes cone to cave in.
5. the fluorescence flow cell of the end face light as described in claim 3 or 4, it is characterized in that, described liquid feeding end (21) seals with catheter (6) further with described outlet end (31) and connects, and the periphery of the end of described catheter (6) is provided with the seal (7) cooperated with described seal.
6. the fluorescence flow cell of end face light as claimed in claim 5, it is characterised in that described seal (7) is made up of elastomeric material.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201620093969.2U CN205538682U (en) | 2016-01-29 | 2016-01-29 | Fluorescence flow -through cell of terminal surface photic |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201620093969.2U CN205538682U (en) | 2016-01-29 | 2016-01-29 | Fluorescence flow -through cell of terminal surface photic |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN205538682U true CN205538682U (en) | 2016-08-31 |
Family
ID=56774173
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201620093969.2U Expired - Fee Related CN205538682U (en) | 2016-01-29 | 2016-01-29 | Fluorescence flow -through cell of terminal surface photic |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN205538682U (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111855767A (en) * | 2020-07-01 | 2020-10-30 | 安徽中医药大学 | Multifunctional flowing thin-layer electrochemical cell and application thereof |
-
2016
- 2016-01-29 CN CN201620093969.2U patent/CN205538682U/en not_active Expired - Fee Related
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111855767A (en) * | 2020-07-01 | 2020-10-30 | 安徽中医药大学 | Multifunctional flowing thin-layer electrochemical cell and application thereof |
| CN111855767B (en) * | 2020-07-01 | 2022-10-11 | 安徽中医药大学 | Multifunctional flowing thin-layer electrochemical cell and application thereof |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| CF01 | Termination of patent right due to non-payment of annual fee | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20160831 Termination date: 20220129 |