CN109279937A - A kind of culture medium of edible fungus solid spawn and preparation method thereof - Google Patents
A kind of culture medium of edible fungus solid spawn and preparation method thereof Download PDFInfo
- Publication number
- CN109279937A CN109279937A CN201810361317.6A CN201810361317A CN109279937A CN 109279937 A CN109279937 A CN 109279937A CN 201810361317 A CN201810361317 A CN 201810361317A CN 109279937 A CN109279937 A CN 109279937A
- Authority
- CN
- China
- Prior art keywords
- culture medium
- bag
- strain
- mycelia
- solid spawn
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Withdrawn
Links
Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G18/00—Cultivation of mushrooms
- A01G18/20—Culture media, e.g. compost
-
- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05D—INORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C; FERTILISERS PRODUCING CARBON DIOXIDE
- C05D3/00—Calcareous fertilisers
Landscapes
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Inorganic Chemistry (AREA)
- Organic Chemistry (AREA)
- Mycology (AREA)
- Environmental Sciences (AREA)
- Mushroom Cultivation (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Abstract
The present invention is a kind of culture medium and preparation method thereof of edible fungus solid spawn, belongs to Spawn incubation field.Peat soil, cotton seed hulls, wheat bran, gypsum, sucrose are prepared enough by formula rate first, then plus lime, water puddle the culture medium that obtained pH value is 6.5~7.0, culture medium is dispensed with strain bag again, every bag of 0.25kg, then carry out high-temperature sterilization, temperature is 121 DEG C~123 DEG C, time is 2.5 hours, access parent species or original seed again after cooling, then strain bag is put in 25 DEG C of darkroom and cultivates mycelia, can remove contamination impurity elimination after cultivating mycelia 7 days, and mycelia purseful after culture mycelia 25~30 days obtains the solid spawn.Culture medium produced by the present invention and not only non-aging, the storage life limit for length with its solid spawn obtained, and manufacture craft is simple, sterilizing require it is low, it is more cost-effective.
Description
Technical field
The present invention relates to the culture medium of a kind of solid spawn of edible mushroom more particularly to a kind of edible fungus solid spawn and its
Preparation method, it is particularly applicable to the Spawn incubations of edible mushroom.
Background technique
Edible mushroom includes the edible mushrooms such as agaricus bisporus, Brazilian mushroom, straw mushroom, coprinus comatus, brown mushroom.For a long time, it eats
Bacterium uses the solid particles such as wheat, grain as bacterium culture medium more, obtained production, cultivation strain be referred to as wheat
Strain, although kernel culture has many advantages, such as that sprouting is fast, growing point is mostly strong with growth potential, its manufacture craft is cumbersome, takes a lot of work
Time-consuming, sterilizing requires height, strain pollution more difficult to control, and the most serious is the aging phenomena that strain easily spits yellow water, to make
It is shorter at storage life, generally 35~45 days.
Summary of the invention
The purpose of the present invention is overcoming, manufacture craft existing in the prior art is cumbersome, sterilizing requirement is high, strain is easy to aging
And defect and problem that storage life is shorter, provide a kind of simple process, sterilizing requires low, the difficult aging of strain, and storage life compared with
A kind of culture medium and preparation method thereof of long edible fungus solid spawn.
In order to achieve the above object, the technical solution of the invention is as follows: a kind of culture medium of edible fungus solid spawn, the training
Support the component and its weight ratio of base are as follows: peat soil 68~78, cotton seed hulls 10~15, wheat bran 10~15, gypsum 1~2, sucrose
1~2 and be added lime adjustment culture medium pH value be 6.5~7.0.
The component and its weight ratio of the culture medium are as follows: peat soil 73, cotton seed hulls 15, wheat bran 10, gypsum 1, sucrose 1
And the pH value that lime adjustment culture medium is added is 7.0.
A kind of preparation method of the edible fungus solid spawn using the culture medium, it is characterised in that as follows into
Row:
(1) it stocks up: preparing enough raw material by output, and peat soil is sieved to remove the sundries that diameter is greater than 1cm, it is desirable that bran
Skin is fresh, without mildew, and cotton seed hulls does not agglomerate agglomerating；
(2) it prepares: mixing peat soil, cotton seed hulls, wheat bran, gypsum and sucrose by the component and its weight ratio of culture medium
Even, and water is added, the weight ratio of raw material and water is 1: 1.2~1: 1.3, adds and carries out mixing to raw material after water and turn so that raw material is inhaled
Water is uniform, while lime is added to adjust pH value, so that the pH value of the culture medium made is 6.5~7.0；
(3) it dispenses: culture medium obtained above being dispensed with strain bag, per packed culture medium 0.25kg, it is desirable that point
Strain bag after dress is full, elastic suitable, and covered plastic lasso seals in strain bag, pays attention to gently taking when contacting strain bag
It puts down gently damaged to prevent strain bag and causes strain pollution；
(4) it sterilizes: high-temperature sterilization being carried out to strain bag obtained above, sterilising temp is 121 DEG C~123 DEG C, and the time is
2.5 hours, strain bag is put into indoor carry out natural cooling with spare after sterilizing；[
(5) it is inoculated with: the seal closure of the strain bag after opening above-mentioned sterilizing in superclean bench or inoculating hood, to obtained
Culture medium carries out aseptic inoculation；When making original seed, the parent species dosage of every bag of access is 1cm square fritter；In production production kind
When, the original seed dosage of every bag of access is 15ml；
(6) it cultivates: the strain bag after inoculation being put in 25 DEG C of darkroom and cultivate mycelia, such as access is parent species, then 5 days
After be colonized material feeding, such as access be original seed, then be colonized material feeding after 3 days；Can remove contamination impurity elimination after cultivating mycelia 7 days, cultivate mycelia 25
Mycelia purseful after~30 days, strain is mature, obtains the solid spawn.
Strain bag used in the packing step is single layer high-pressure polypropylene strain bag.
Compared with prior art, the invention has the benefit that
1. the raw material as used in a kind of culture medium of edible fungus solid spawn of the present invention and preparation method thereof only wraps
Peat soil, cotton seed hulls, wheat bran, gypsum and sucrose, and the lime of adjustment pH value are included, therefore when making the present invention, is not needed
Immersion or boiling, and general kernel culture must carry out immersion or boiling in production to wheat, otherwise wheat is difficult to reach
Suitable water content, and the duration and degree of heating is got hold of in immersion, boiling, it is careless slightly, it leads to the failure, in addition, the Sheng of kernel culture
Packaging container is generally vial, and volume weight is big, inconvenient for use, and strain bag used in it requires to be double-ply bag, process is loaded down with trivial details,
It is time-consuming, and the present invention's is single layer strain bag.Therefore present invention process is simple and convenient to operate, saving of work and time.
2. the raw material as used in a kind of culture medium of edible fungus solid spawn of the present invention and preparation method thereof only wraps
Peat soil, cotton seed hulls, wheat bran, gypsum and sucrose are included, and adjusts the lime of pH value, and original used in general kernel culture
The nutrition of material is extremely abundant, easily causes to pollute, therefore long to the sterilization time of kernel culture, generally at 4 more than hour, and
Sterilization time of the invention is generally 2.5 hours, thus sterilizing of the invention require it is low, not easy to pollute.
3. the solid spawn as obtained by a kind of culture medium of edible fungus solid spawn of the present invention and preparation method thereof protects
Depositing the phase is 60~70 days, and general kernel culture is easy to aging, can spit yellow water phenomenon after cultural hypha 30 days, therefore
Storage life of the invention is longer.
4. the primary raw material as used in a kind of culture medium of edible fungus solid spawn of the present invention and preparation method thereof
For peat soil, peat soil is formed through many years spontaneous fermentation, and rotten degree is good, and retention ability is strong, and fertility is suitable for that furthermore China's peat soil provides
Source is abundant, cheap, and peat soil price is 250~300 yuan/m3, is converted into 0.25~0.30 yuan/liter, every liter of raw material can be made
Make 2 bottles of solid spawn, and the cost of material of the kernel culture of same volume is 0.40~0.50 yuan, thus, use peat soil for
Major ingredient makes solid spawn, and cost of material reduces about 40% than kernel culture, in addition since peat soil need not pretreatment
Directly use, and then make manufacture craft simplification, produce the expenses reduction such as required water, electricity, artificial, therefore the strain that the present invention makes
Production cost 50% is at least reduced than kernel culture, therefore the present invention saves cost.
Specific embodiment
Below in conjunction with specific embodiment, the present invention will be described in detail:
A kind of culture medium of edible fungus solid spawn of the invention, the component and its weight ratio of the culture medium are as follows: mud
Charcoal soil 68~78, cotton seed hulls 10~15, wheat bran 10~15, gypsum 1~2, sucrose 1~2 and the pH that lime adjustment culture medium is added
Value is 6.5~7.0.
The component and its weight ratio of the culture medium are as follows: peat soil 73, cotton seed hulls 15, wheat bran 10, gypsum 1, sucrose 1
And the pH value that lime adjustment culture medium is added is 7.0.
The constituent and Action Specification of above-mentioned culture medium are as follows:
Peat soil: as the major ingredient in solid spawn of the present invention, it is formed through many years spontaneous fermentation, and rotten degree is good, water holding
Power is strong；Fertility is suitable for that the peat soil organic matter average content saved containing a large amount organic matter, such as Jilin Province, China is 65.4%, Chuan Xi
Northern plateau is 57.5%, and the content of nitrogen is also quite abundant in peat soil, Peat from Northeast China soil nitrogen content average out to 1.5~
2.0%, 2.5% is reached as high as, in addition, the content of phosphorus is also above the minerals soil body, about 0.29~0.33% in peat soil, very
Be adapted as straw rotting fungus class, such as agaricus bisporus, straw mushroom, Brazilian mushroom fruiting cover soil material.
Cotton seed hulls: graininess is good, can enhance the gas permeability of compost, with the growth of sharp mycelia.
Wheat bran: vitamin and nitrogen source are provided for compost.
Sucrose: quick-acting carbon sources are provided for compost.
Gypsum: for compost supplementing calcium element.
Lime: for compost supplementing calcium element and adjustment pH value.
The present invention proposes a kind of preparation method of edible fungus solid spawn using the culture medium in turn, this method according to
It is secondary the following steps are included:
(1) it stocks up: preparing enough raw material by output, and peat soil is sieved to remove the sundries that diameter is greater than 1cm, it is desirable that bran
Skin is fresh, without mildew, and cotton seed hulls does not agglomerate agglomerating；
(2) it prepares: mixing peat soil, cotton seed hulls, wheat bran, gypsum and sucrose by the component and its weight ratio of culture medium
Even, and water is added, the weight ratio of raw material and water is 1: 1.2~1: 1.3, adds and carries out mixing to raw material after water and turn so that raw material is inhaled
Water is uniform, while lime is added to adjust pH value, so that the pH value of the culture medium made is 6.5~7.0；
(3) it dispenses: culture medium obtained above being dispensed with strain bag, per packed culture medium 0.25kg, it is desirable that point
Strain bag after dress is full, elastic suitable, and covered plastic lasso seals in strain bag, pays attention to gently taking when contacting strain bag
It puts down gently damaged to prevent strain bag and causes strain pollution；
(4) it sterilizes: high-temperature sterilization being carried out to strain bag obtained above, sterilising temp is 121 DEG C~123 DEG C, and the time is
2.5 hours, strain bag is put into indoor carry out natural cooling with spare after sterilizing；
(5) it is inoculated with: the seal closure of the strain bag after opening above-mentioned sterilizing in superclean bench or inoculating hood, to obtained
Culture medium carries out aseptic inoculation；When making original seed, the parent species dosage of every bag of access is 1cm square fritter；In production production kind
When, the original seed dosage of every bag of access is 15ml；
(6) it cultivates: the bacterium bag after inoculation being put in 25 DEG C of darkroom and cultivate mycelia, such as access is parent species, then after 5 days
It is colonized material feeding, such as access is original seed, then is colonized material feeding after 3 days；Can remove contamination impurity elimination after cultivating mycelia 7 days, and culture mycelia 25~
Mycelia purseful after 30 days, strain is mature, obtains the solid spawn.
Strain bag used in the packing step is single layer high-pressure polypropylene strain bag.
It is parent species as accessed in the medium, then culture covers with mycelia up to original seed, also referred to as second level kind.
It is original seed as accessed in the medium, then culture covers with mycelia up to cultivar, also referred to as three-level kind, production kind
Or cultivar.
Embodiment 1:
A kind of culture medium of the solid spawn of edible mushroom, the component and its weight ratio of the culture medium are as follows: peat soil 68,
Cotton seed hulls 15, wheat bran 15, gypsum 1, sucrose 1 and the pH value that lime adjustment culture medium is added are 7.0.
A kind of preparation method of the edible fungus solid spawn using the culture medium, the production method as follows into
Row:
(1) it stocks up: preparing enough raw material by output, and peat soil is sieved to remove the sundries that diameter is greater than 1cm, it is desirable that bran
Skin is fresh, without mildew, and cotton seed hulls does not agglomerate agglomerating；
(2) it prepares: mixing peat soil, cotton seed hulls, wheat bran, gypsum and sucrose by the component and its weight ratio of culture medium
Even, and water is added, the weight ratio of raw material and water is 1: 1.2~1: 1.3, adds and carries out mixing to raw material after water and turn so that raw material is inhaled
Water is uniform, while lime is added to adjust pH value, so that the pH value of the culture medium made is 7.0；
(3) it dispenses: culture medium obtained above being dispensed with strain bag, per packed culture medium 0.25kg, it is desirable that point
Strain bag after dress is full, elastic suitable, and covered plastic lasso seals in strain bag, pays attention to gently taking when contacting strain bag
It puts down gently damaged to prevent strain bag and causes strain pollution；
(4) it sterilizes: high-temperature sterilization being carried out to strain bag obtained above, sterilising temp is 121 DEG C~123 DEG C, and the time is
2.5 hours, strain bag is put into indoor carry out natural cooling with spare after sterilizing；
(5) it is inoculated with: the seal closure of the strain bag after opening above-mentioned sterilizing in superclean bench or inoculating hood, to strain bag
Interior culture medium carries out aseptic inoculation, and access is parent species, and the parent species dosage of every bag of access is 1cm square fritter.
(6) it cultivates: the bacterium bag after inoculation being put in 25 DEG C of darkroom and cultivate mycelia, material feeding is colonized after 5 days, cultivate mycelia
Can remove contamination impurity elimination after 7 days, and mycelia purseful after culture mycelia 25~30 days, strain is mature, obtain the solid spawn.
Embodiment 2:
A kind of culture medium of the solid spawn of edible mushroom, the component and its weight ratio of the culture medium are as follows: peat soil 78,
Cotton seed hulls 10, wheat bran 10, gypsum 1, sucrose 1 and the pH value that lime adjustment culture medium is added are 6.8.
A kind of preparation method of the edible fungus solid spawn using the culture medium, the production method as follows into
Row:
(1) it stocks up: preparing enough raw material by output, and peat soil is sieved to remove the sundries that diameter is greater than 1cm, it is desirable that bran
Skin is fresh, without mildew, and cotton seed hulls does not agglomerate agglomerating；
(2) it prepares: mixing peat soil, cotton seed hulls, wheat bran, gypsum and sucrose by the component and its weight ratio of culture medium
Even, and water is added, the weight ratio of raw material and water is 1: 1.2~1: 1.3, adds and carries out mixing to raw material after water and turn so that raw material is inhaled
Water is uniform, while lime is added to adjust pH value, so that the pH value of the culture medium made is 6.8；
(3) it dispenses: culture medium obtained above being dispensed with strain bag, per packed culture medium 0.25kg, it is desirable that point
Strain bag after dress is full, elastic suitable, and covered plastic lasso seals in strain bag, pays attention to gently taking when contacting strain bag
It puts down gently damaged to prevent strain bag and causes strain pollution；
(4) it sterilizes: high-temperature sterilization being carried out to strain bag obtained above, sterilising temp is 121 DEG C~123 DEG C, and the time is
2.5 hours, strain bag is put into indoor carry out natural cooling with spare after sterilizing；
(5) it is inoculated with: the seal closure of the strain bag after opening above-mentioned sterilizing in superclean bench or inoculating hood, to strain bag
Interior culture medium carries out aseptic inoculation, and access is original seed, and the original seed dosage of every bag of access is 15ml.
(6) it cultivates: the bacterium bag after inoculation being put in 25 DEG C of darkroom and cultivate mycelia, material feeding is colonized after 3 days, cultivate mycelia
Can remove contamination impurity elimination after 7 days, and mycelia purseful after culture mycelia 25~30 days, strain is mature, obtain the solid spawn.
Embodiment 3:
A kind of culture medium of the solid spawn of edible mushroom, the component and its weight ratio of the culture medium are as follows: peat soil 72,
Cotton seed hulls 12, wheat bran 13, gypsum 1, sucrose 2 and the pH value that lime adjustment culture medium is added are 6.5.
A kind of preparation method of the edible fungus solid spawn using the culture medium, the production method as follows into
Row:
(1) it stocks up: preparing enough raw material by output, and peat soil is sieved to remove the sundries that diameter is greater than 1cm, it is desirable that bran
Skin is fresh, without mildew, and cotton seed hulls does not agglomerate agglomerating；
(2) it prepares: mixing peat soil, cotton seed hulls, wheat bran, gypsum and sucrose by the component and its weight ratio of culture medium
Even, and water is added, the weight ratio of raw material and water is 1: 1.2~1: 1.3, adds and carries out mixing to raw material after water and turn so that raw material is inhaled
Water is uniform, while lime is added to adjust pH value, so that the pH value of the culture medium made is 6.5；
(3) it dispenses: culture medium obtained above being dispensed with strain bag, per packed culture medium 0.25kg, it is desirable that point
Strain bag after dress is full, elastic suitable, and covered plastic lasso seals in strain bag, pays attention to gently taking when contacting strain bag
It puts down gently damaged to prevent strain bag and causes strain pollution；
(4) it sterilizes: high-temperature sterilization being carried out to strain bag obtained above, sterilising temp is 121 DEG C~123 DEG C, and the time is
2.5 hours, strain bag is put into indoor carry out natural cooling with spare after sterilizing；
(5) it is inoculated with: the seal closure of the strain bag after opening above-mentioned sterilizing in superclean bench or inoculating hood, to strain bag
Interior culture medium carries out aseptic inoculation, and access is parent species, and the parent species dosage of every bag of access is 1cm square fritter.
(6) it cultivates: the bacterium bag after inoculation being put in 25 DEG C of darkroom and cultivate mycelia, material feeding is colonized after 5 days, cultivate mycelia
Can remove contamination impurity elimination after 7 days, and mycelia purseful after culture mycelia 25~30 days, strain is mature, obtain the solid spawn.
Embodiment 4:
A kind of culture medium of the solid spawn of edible mushroom, the component and its weight ratio of the culture medium are as follows: peat soil 73,
Cotton seed hulls 15, wheat bran 10, gypsum 1, sucrose 1 and the pH value that lime adjustment culture medium is added are 7.0.
A kind of preparation method of the edible fungus solid spawn using the culture medium, the production method as follows into
Row:
(1) it stocks up: preparing enough raw material by output, and peat soil is sieved to remove the sundries that diameter is greater than 1cm, it is desirable that bran
Skin is fresh, without mildew, and cotton seed hulls does not agglomerate agglomerating；
(2) it prepares: mixing peat soil, cotton seed hulls, wheat bran, gypsum and sucrose by the component and its weight ratio of culture medium
Even, and water is added, the weight ratio of raw material and water is 1: 1.2~1: 1.3, adds and carries out mixing to raw material after water and turn so that raw material is inhaled
Water is uniform, while lime is added to adjust pH value, so that the pH value of the culture medium made is 7.0；
(3) it dispenses: culture medium obtained above being dispensed with strain bag, per packed culture medium 0.25kg, it is desirable that point
Strain bag after dress is full, elastic suitable, and covered plastic lasso seals in strain bag, pays attention to gently taking when contacting strain bag
It puts down gently damaged to prevent strain bag and causes strain pollution；
(4) it sterilizes: high-temperature sterilization being carried out to strain bag obtained above, sterilising temp is 121 DEG C~123 DEG C, and the time is
2.5 hours, strain bag is put into indoor carry out natural cooling with spare after sterilizing；
(5) it is inoculated with: the seal closure of the strain bag after opening above-mentioned sterilizing in superclean bench or inoculating hood, to strain bag
Interior culture medium carries out aseptic inoculation, and access is original seed, and the original seed dosage of every bag of access is 15ml；
(6) it cultivates: the bacterium bag after inoculation being put in 25 DEG C of darkroom and cultivate mycelia, material feeding is colonized after 3 days, cultivate mycelia
Can remove contamination impurity elimination after 7 days, and mycelia purseful after culture mycelia 25~30 days, strain is mature, obtain the solid spawn.
A kind of culture medium of edible fungus solid spawn of the present invention and preparation method thereof be suitable for various edible mushrooms such as agaricus bisporus,
The culture of the edible mushrooms such as straw mushroom, coprinus comatus, brown mushroom, Brazilian mushroom, that is, Agricus blazei.
General kernel culture spits yellow water after cultural hypha 30 days, shows the easy aging of its mycelia, not storage endurance, and adopts
The solid spawn made from production method of the present invention have no spits yellow water phenomenon generation, the strain shelf-life up to 60~70 days, and
And no significant difference in terms of its after planting mycelium germination, field planting, fructification can normally form, product quality, yield are suitable,
5 standard baskets are tested, every basket of area is 0.205m2, as a result produces Brazilian mushroom fresh weight 9.18Kg, converts into per unit area yield and calculates are as follows:
9.18Kg ÷ (0.205m2/ baskets × 5 baskets)=8.95Kg/m2, product quality indicator are without exception.
Following table is to carry out the applicable cases of more various cultivation strains by taking Brazilian mushroom as an example:
Four kinds of Brazilian mushroom cultivar applicable cases compare
Claims (4)
1. a kind of culture medium of edible fungus solid spawn, it is characterised in that: the component and its weight ratio of the culture medium are as follows: mud
Charcoal soil 68~78, cotton seed hulls 10~15, wheat bran 10~15, gypsum 1~2, sucrose 1~2 and the pH that lime adjustment culture medium is added
Value is 6.5~7.0.
2. a kind of culture medium of edible fungus solid spawn according to claim 1, it is characterised in that: the group of the culture medium
Point and its weight ratio are as follows: lime adjustment culture medium is simultaneously added in peat soil 73, cotton seed hulls 15, wheat bran 10, gypsum 1, sucrose 1
PH value is 7.0.
3. it is a kind of using culture medium as claimed in claim 1 or 2 preparation solid spawn method, it is characterised in that as follows into
Row:
(1) it stocks up: preparing enough raw material by output, and peat soil is sieved to remove the sundries that diameter is greater than 1cm, it is desirable that wheat bran is new
It is fresh, without mildew, cotton seed hulls does not agglomerate agglomerating；
(2) it prepares: mixing peat soil, cotton seed hulls, wheat bran, gypsum and sucrose by the component and its weight ratio of culture medium,
And water is added, the weight ratio of raw material and water is 1:1.2~1:1.3, adds and carries out mixing to raw material after water and turn so that raw material absorbs water
Uniformly, while lime is added to adjust pH value, so that the pH value of the culture medium made is 6.5~7.0；
(3) culture medium obtained above is dispensed with strain bag, per packed culture medium 0.25kg, it is desirable that the strain after packing
Bag is full, elastic to be suitable for, and covered plastic lasso seals in strain bag, pays attention to handling with care with fungi-proofing when contacting strain bag
Kind bag is damaged and causes strain pollution；
(4) high-temperature sterilization is carried out to strain bag obtained above, sterilising temp is 121 DEG C~123 DEG C, and the time is 2.5 hours, is gone out
Strain bag is put into indoor carry out natural cooling with spare after bacterium；
(5): the seal closure of the strain bag after opening above-mentioned sterilizing in superclean bench or inoculating hood, to culture medium obtained into
Row aseptic inoculation；When making original seed, the parent species dosage of every bag of access is 1cm square fritter；In production production kind, every bag is connect
The original seed dosage entered is 15ml；
(6): the strain bag after inoculation being put in 25 DEG C of darkroom and cultivate mycelia, such as access is parent species, then is colonized and eats after 5 days
Material；If access is original seed, then material feeding is colonized after 3 days；Can remove contamination impurity elimination after cultivating mycelia 7 days, after culture mycelia 25~30 days
Mycelia purseful, strain is mature, obtains the solid spawn.
4. a kind of method using culture medium preparation solid spawn according to claim 3, it is characterised in that: the packing
Strain bag used in step is single layer high-pressure polypropylene strain bag.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201810361317.6A CN109279937A (en) | 2018-04-20 | 2018-04-20 | A kind of culture medium of edible fungus solid spawn and preparation method thereof |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201810361317.6A CN109279937A (en) | 2018-04-20 | 2018-04-20 | A kind of culture medium of edible fungus solid spawn and preparation method thereof |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN109279937A true CN109279937A (en) | 2019-01-29 |
Family
ID=65186202
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201810361317.6A Withdrawn CN109279937A (en) | 2018-04-20 | 2018-04-20 | A kind of culture medium of edible fungus solid spawn and preparation method thereof |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN109279937A (en) |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN112931050A (en) * | 2021-03-09 | 2021-06-11 | 福贡九源农业发展有限责任公司 | Edible fungus culture medium and preparation method thereof |
| CN116982516A (en) * | 2023-09-27 | 2023-11-03 | 国合(青岛)循环经济有限公司 | Cultivation technology of microbiota in tremella aurantialba mixed culture |
-
2018
- 2018-04-20 CN CN201810361317.6A patent/CN109279937A/en not_active Withdrawn
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN112931050A (en) * | 2021-03-09 | 2021-06-11 | 福贡九源农业发展有限责任公司 | Edible fungus culture medium and preparation method thereof |
| CN116982516A (en) * | 2023-09-27 | 2023-11-03 | 国合(青岛)循环经济有限公司 | Cultivation technology of microbiota in tremella aurantialba mixed culture |
| CN116982516B (en) * | 2023-09-27 | 2023-12-15 | 国合(青岛)循环经济有限公司 | Cultivation technology of microbiota in tremella aurantialba mixed culture |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN103404370B (en) | The method of edible fungus species is cultivated with pelelith | |
| CN1879463A (en) | A culture medium for golden mushroom and golden mushroom plantation method | |
| CN101717309B (en) | Culture medium for straw rotting edible fungi solid strain and method for preparing solid strain | |
| KR101645802B1 (en) | Cultivating method of mushroom using sawdust media | |
| CN102498937B (en) | Method for culturing oyster mushroom | |
| CN102487727A (en) | Method for producing pleurotus eryngii quel strains | |
| CN102630483A (en) | Method for shortening cultivation period of needle mushroom during production | |
| CN111328627A (en) | Direct regeneration culture method for tremella aurantialba sporocarp | |
| CN109997608A (en) | A kind of implantation methods of edible mushroom | |
| CN107135805A (en) | A kind of asparagus ecology planting method based on high nutrition | |
| JP2012055309A (en) | Method for producing liquid spawn of mushroom | |
| CN109279937A (en) | A kind of culture medium of edible fungus solid spawn and preparation method thereof | |
| CN104303840A (en) | Cultivating method for tray-loaded flammulina velutipes | |
| CN106431658B (en) | Oyster mushroom culture medium and oyster mushroom cultivation method | |
| KR101687892B1 (en) | Cultivating method of pleurotus eryngii and the composition of cultur medium | |
| CN104718995A (en) | Method for producing solid liquefied strains of oyster mushrooms | |
| CN111587738A (en) | Pleurotus cornucopiae packing and briquetting production method | |
| CN103918481A (en) | Liquid pholiota adipose strain mixing cultivation process | |
| JP2015181413A (en) | Indoor artificial cultivation method for leucopaxillus giganteus | |
| CN110150029A (en) | Application of the Chinese redbud in terms of edible fungus culturing | |
| KR101687891B1 (en) | Cultivating method of tree ear and the composition of cultur medium | |
| KR101687890B1 (en) | Cultivating method of oyster mushroomr and the composition of culture medium | |
| CN110637673A (en) | Industrial cultivation process of Dictyophora rubrovalvata | |
| CN104145711A (en) | Grifola frondosa three-stage culture production method | |
| CN112931050A (en) | Edible fungus culture medium and preparation method thereof |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| WW01 | Invention patent application withdrawn after publication |
Application publication date: 20190129 |
|
| WW01 | Invention patent application withdrawn after publication |