CN109275378A - A kind of sour processing method for promoting tuniclike psammosilene root seed to sprout - Google Patents
A kind of sour processing method for promoting tuniclike psammosilene root seed to sprout Download PDFInfo
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- CN109275378A CN109275378A CN201811366899.3A CN201811366899A CN109275378A CN 109275378 A CN109275378 A CN 109275378A CN 201811366899 A CN201811366899 A CN 201811366899A CN 109275378 A CN109275378 A CN 109275378A
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- seed
- acid
- tuniclike psammosilene
- psammosilene root
- sprout
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01C—PLANTING; SOWING; FERTILISING
- A01C1/00—Apparatus, or methods of use thereof, for testing or treating seed, roots, or the like, prior to sowing or planting
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01C—PLANTING; SOWING; FERTILISING
- A01C1/00—Apparatus, or methods of use thereof, for testing or treating seed, roots, or the like, prior to sowing or planting
- A01C1/08—Immunising seed
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- Environmental Sciences (AREA)
- Pretreatment Of Seeds And Plants (AREA)
Abstract
The invention discloses a kind of sour processing methods that promotion tuniclike psammosilene root seed is sprouted, method includes the following steps: (1) chooses the seed of mature and plump, room temperature clear water impregnates 12h, makes seed water swelling, obtain seed I；(2) seed I is carried out different acid to handle, obtains seed II；(3) seed II is carried out disinfection processing, obtains seed III；(4) seed III is placed in the 9cm culture dish for being covered with 2 layers of filter paper and is sprouted.The result shows that 15% salt acid soak 30min, 15% sulfuric acid impregnate 45min and 20% lemon acid soak 15min germination rate has been respectively increased 53%, 25%, 17% compared with control (handling without acid).This method can effectively improve tuniclike psammosilene root seed germination rate, lay a good foundation for scale sexual reproduction technique.
Description
Technical field
The present invention relates to the sour processing method that tuniclike psammosilene root seed is sprouted, especially a kind of acid for promoting tuniclike psammosilene root seed to sprout
Processing method.
Background technique
Tuniclike psammosilene root (Psammosilene tunicoides W.C.Wu et C.Y.Wu) is Caryophyllaceae gold genus list category
Kind plant.Main product is saved in Yunnan, Sichuan, Guizhou and Tibet etc., is used as medicine with root, for rheumatism, stomachache with cool feeling, traumatic injury and outer
Hurting bleeding etc. has good anti-inflammatory analgetic to act on, be " Yunnan Baiyao " series of products longstalk condorvine herb dissipate, Yunnan hongyao capsule, pain blood health
One of main components of a variety of well-known Chinese patent drugs such as capsule, Guizhou " Jin Gulian " capsule.
Currently, tuniclike psammosilene root medicinal material is mainly derived from wild resource.Since tuniclike psammosilene root is harsher to habitat requirement, divide naturally
Cloth region is narrow, and in recent years, with the continuous rising of the market demand, tuniclike psammosilene root is excavated by predatoriness, cause its habitat destruction,
Population quantity reduces sharply, and is included in precious rare endangered species by " Chinese Plants Red Data Book ", belongs to national second level and lays special stress on protecting plant
Object.Medical value, the market demand and Endangered based on tuniclike psammosilene root, artificial growth tuniclike psammosilene root, which becomes, solves having for the market demand
Effect means.And in tuniclike psammosilene root reproductive process, seed sprouting is key, directly affects emergence and yield.
Tuniclike psammosilene root seed is in long inverted ovoid, there is pericarp coating outside seed, more folds, easily carries fungi and bacterium secretly, outside seed
The mechanical restriction for enclosing pericarp can inhibit the growth of radicle, therefore germination rate is low under natural conditions, and seedling-growing time is long and perishable, this
Seriously constrain the scale and industrialization of artificial growth.
Summary of the invention
The present invention provides a kind of sour processing method that promotion tuniclike psammosilene root seed is sprouted.Operation of the present invention is simple, it is at low cost,
Tuniclike psammosilene root seed germination rate can be effectively improved and cultivation period is short, the resource regeneration and sustainable use to the species provide technology
It supports.
Technical solution of the present invention:
A kind of sour processing method for promoting tuniclike psammosilene root seed to sprout passes through variety classes acid, various concentration and soaking time
Processing promotes tuniclike psammosilene root seed to sprout, the specific steps are as follows:
Step 1: choosing the tuniclike psammosilene root seed of mature and plump, room temperature clear water impregnates 10-14h, makes seed water swelling, obtain
Seed I；
Step 2: seed I being subjected to sour processing, obtains seed II；
Step 3: seed II is carried out disinfection processing；Obtain seed III；
Step 4: seed III being placed in the 9cm culture dish for being covered with 2 layers of filter paper, cultivate 30-40d, during which observe daily simultaneously
Sprouting situation is recorded, and counts germination rate.
The sour processing method above-mentioned for promoting tuniclike psammosilene root seed to sprout, it is described that seed I is subjected to sour processing in step 2
It is: sour processing is carried out to seed I with hydrochloric acid, sulfuric acid or citric acid.
It is above-mentioned promote tuniclike psammosilene root seed sprout sour processing method, it is described with hydrochloric acid to seed I carry out acid processing be；
With the salt acid soak 15-30min of concentration 5%-15%.
It is above-mentioned promote tuniclike psammosilene root seed sprout sour processing method, it is described with hydrochloric acid to seed I carry out acid processing be；
With the salt acid soak 30min of concentration 15%.
It is above-mentioned promote tuniclike psammosilene root seed sprout sour processing method, it is described with sulfuric acid to seed I carry out acid processing be；
15-45min is impregnated with the sulfuric acid that concentration is 5%-15%.
It is above-mentioned promote tuniclike psammosilene root seed sprout sour processing method, it is described with sulfuric acid to seed I carry out acid processing be；
45min is impregnated with the sulfuric acid that concentration is 15%.
The sour processing method above-mentioned for promoting tuniclike psammosilene root seed to sprout, it is described that sour processing is carried out to seed I with citric acid
It is；The lemon acid soak 15-45min for being 5%-20% with concentration.
The sour processing method above-mentioned for promoting tuniclike psammosilene root seed to sprout, it is described that sour processing is carried out to seed I with citric acid
It is；The lemon acid soak 15min for being 20% with concentration.
The sour processing method above-mentioned for promoting tuniclike psammosilene root seed to sprout, it is described that seed II carries out disinfection place in step 3
Reason is；With 75% alcohol disinfecting 30s, aseptic water washing 3 times, then 30s, aseptic water washing 3 are sterilized with 0.2% potassium permanganate
It is secondary, 10min finally is handled with 0.2% mercuric chloride, with aseptic water washing 5 times.
The sour processing method above-mentioned for promoting tuniclike psammosilene root seed to sprout, in step 4, condition of culture is: temperature is 25 ± 3
DEG C, relative humidity 70%-80%.
What the present invention reached has the beneficial effect that:
(1) easy to operate by impregnating tuniclike psammosilene root seed under acid not of the same race and concentration, it is at low cost, germination rate is effectively improved,
Its breeding coefficient is increased, cultivation period is shortened.
(2) technical support is provided to the resource regeneration of tuniclike psammosilene root and sustainable use.
Detailed description of the invention
Fig. 1 is the influence that different disposal sprouts tuniclike psammosilene root seed.Wherein, A:20% citric acid treatment 15min is handled；Place
Manage B:15% sulfuric acid treatment 45min；Handle C:15% HCl treatment 30min；Handle D: comparative example.
Specific embodiment
The present invention is described in further detail with reference to the accompanying drawings and examples.
Embodiment 1.
A kind of sour processing method for promoting tuniclike psammosilene root seed to sprout, the specific steps are as follows:
(1) explant rinses: choosing the seed of mature and plump, room temperature clear water impregnates 12h, makes seed water swelling, obtain
Seed I；(2) acid processing: after the citric acid of seed I, sulfuric acid or HCl treatment, 10min is rinsed with flowing water, obtains seed
Ⅱ。
(3) disinfect: by the seed II with 75% alcohol disinfecting 30s, aseptic water washing 3 times, then with 0.2%
Potassium permanganate sterilizes 30s, aseptic water washing 3 times, is finally obtained with 0.2% mercuric chloride processing 10min with aseptic water washing 5 times
Seed III.
(4) seed III is placed in the 9cm culture dish for being covered with 2 layers of filter paper and is sprouted, condition of culture is temperature (25 ± 3
DEG C), relative humidity (70%-80%).Sprouting situation is observed and recorded daily, counts germination rate after 30d.
Tuniclike psammosilene root seed sprouts suitable citric acid treatment: concentration 20%, time 15min.As shown in table 1 and Fig. 1.Fig. 1
In, A is citric acid treatment (concentration 20%, time 15min).
Tuniclike psammosilene root seed sprouts suitable sulfuric acid treatment: concentration 15%, time 45min.As shown in table 1 and Fig. 1.In Fig. 1,
B is sulfuric acid treatment (concentration 15%, time 45min).
Tuniclike psammosilene root seed sprouts optimum HCl treatment: concentration 15%, time 30min.As shown in table 1 and Fig. 1.Fig. 1
In, C is HCl treatment (concentration 15%, time 30min).
Embodiment 2: citric acid treatment
The seed of mature and plump is chosen, room temperature clear water impregnates 12h, makes seed water swelling, obtain seed I；Seed I is divided
Not Yong concentration be 5% and 20% lemon acid soak 15min, 45min, after processing with flowing water rinse 10min, obtain seed II；
By seed II with 75% alcohol disinfecting 30s, aseptic water washing 3 times, then with 0.2% potassium permanganate sterilize 30s, sterile water punching
It washes 3 times, finally obtains seed III with aseptic water washing 5 times with 0.2% mercuric chloride processing 10min；The seed III is placed in
It is covered in the 9cm culture dish of 2 layers of filter paper, condition of culture is that temperature is 25 ± 3 DEG C, relative humidity 70%-80%, be the results are shown in Table
1。
Embodiment 3: sulfuric acid treatment
The seed of mature and plump is chosen, room temperature clear water impregnates 12h, makes seed water swelling, obtain seed I；Seed I is divided
Not Yong concentration be 5% and 15% sulfuric acid impregnate 15min, 45min, after processing with flowing water rinse 10min, obtain seed II；It will
Seed II with 75% alcohol disinfecting 30s, aseptic water washing 3 times, then with 0.2% potassium permanganate sterilize 30s, aseptic water washing
3 times, seed III finally is obtained with aseptic water washing 5 times with 0.2% mercuric chloride processing 10min；The seed III is placed in paving
In the 9cm culture dish for having 2 layers of filter paper, condition of culture is 25 ± 3 DEG C of temperature, relative humidity 70%-80%, the results are shown in Table 1.
Embodiment 4: HCl treatment
The seed of mature and plump is chosen, room temperature clear water impregnates 12h, makes seed water swelling, obtain seed I；Seed I is divided
Not Yong concentration be 5% and 15% salt acid soak 15min, 30min, after processing with flowing water rinse 10min, obtain seed II；It will
Seed II with 75% alcohol disinfecting 30s, aseptic water washing 3 times, then with 0.2% potassium permanganate sterilize 30s, aseptic water washing
3 times, seed III finally is obtained with aseptic water washing 5 times with 0.2% mercuric chloride processing 10min；The seed III is placed in paving
In the 9cm culture dish for having 2 layers of filter paper, condition of culture is 25 ± 3 DEG C of temperature, relative humidity 70%-80%, as a result see the table below 1 institute
Show.
Comparative example
The seed of mature and plump is chosen, room temperature clear water impregnates 12h, makes seed water swelling, obtain seed I；By seed I in
In sterile water impregnate acid processing same time obtain seed II, by seed II with 75% alcohol disinfecting 30s, aseptic water washing 3
It is secondary, then 30s is sterilized with 0.2% potassium permanganate, aseptic water washing 3 times, 10min finally is handled with 0.2% mercuric chloride, use is sterile
Water rinses 5 times, obtains seed II；Seed II is placed in the 9cm culture dish for being covered with 2 layers of filter paper, condition of culture is temperature 25 ± 3
DEG C, relative humidity 70%-80%, the results are shown in Table 1.
1 seed of table sprouts the table of comparisons
Note: different lowercases indicate that the two has significant difference (P < 0.05) in table.
As shown in table 1, the acid of suitable concentration and time treated tuniclike psammosilene root seed, germination rate and untreated seed
Compared to being improved.Tuniclike psammosilene root seed sprouts optimum HCl treatment are as follows: concentration 15%, time 30min；Optimum sulfuric acid
Processing are as follows: concentration 15%, time 45min；Optimum citric acid treatment are as follows: concentration 20%, time 15min.Compared with the control,
Germination rate has been respectively increased 53%, 25%, 17%.In addition, the facilitation effect that above-mentioned 3 kinds of acid sprouts tuniclike psammosilene root seed are as follows: salt
Sour > sulfuric acid > citric acid, and handle the time are as follows: sulfuric acid > hydrochloric acid > citric acid, therefore when handling tuniclike psammosilene root seed, specifically
Any method is selected, it can optimum selecting according to the actual situation.This method is easy to operate, practical, to the Precious, Rare, Endangered object
The protection and continuous utilization of kind wild resource provide technical support.
It is noted that without departing from the principle of the present invention, may be used also for the research staff of this field
To make several improvement, these improvement are all belonged to the scope of protection of the present invention.Therefore, the scope of protection of the patent of the present invention should be with institute
Subject to attached claim.
Claims (10)
1. it is a kind of promote tuniclike psammosilene root seed sprout sour processing method, it is characterised in that: by variety classes acid, various concentration and
Soaking time processing promotes tuniclike psammosilene root seed to sprout, the specific steps are as follows:
Step 1: choosing the tuniclike psammosilene root seed of mature and plump, room temperature clear water impregnates 10-14h, makes seed water swelling, obtain seed
Ⅰ；
Step 2: seed I being subjected to sour processing, obtains seed II；
Step 3: seed II is carried out disinfection processing；Obtain seed III；
Step 4: seed III being placed in the 9cm culture dish for being covered with 2 layers of filter paper, cultivate 30-40d, during which observe and record daily
Sprouting situation, and count germination rate.
2. the sour processing method for promoting tuniclike psammosilene root seed to sprout as described in claim 1, it is characterised in that: described in step 2
Seed I is carried out acid processing be: sour processing is carried out to seed I with hydrochloric acid, sulfuric acid or citric acid.
3. the sour processing method for promoting tuniclike psammosilene root seed to sprout as claimed in claim 2, it is characterised in that: described uses hydrochloric acid
Carrying out acid processing to seed I is；With the salt acid soak 15-30min of concentration 5%-15%.
4. the sour processing method for promoting tuniclike psammosilene root seed to sprout as claimed in claim 3, it is characterised in that: described uses hydrochloric acid
Carrying out acid processing to seed I is；With the salt acid soak 30min of concentration 15%.
5. the sour processing method for promoting tuniclike psammosilene root seed to sprout as claimed in claim 2, it is characterised in that: described uses sulfuric acid
Carrying out acid processing to seed I is；15-45min is impregnated with the sulfuric acid that concentration is 5%-15%.
6. the sour processing method for promoting tuniclike psammosilene root seed to sprout as claimed in claim 5, it is characterised in that: described uses sulfuric acid
Carrying out acid processing to seed I is；45min is impregnated with the sulfuric acid that concentration is 15%.
7. the sour processing method for promoting tuniclike psammosilene root seed to sprout as claimed in claim 2, it is characterised in that: described uses lemon
Acid carries out acid processing to seed I；The lemon acid soak 15-45min for being 5%-20% with concentration.
8. the sour processing method for promoting tuniclike psammosilene root seed to sprout as claimed in claim 7, it is characterised in that: described uses lemon
Acid carries out acid processing to seed I；The lemon acid soak 15min for being 20% with concentration.
9. the sour processing method for promoting tuniclike psammosilene root seed to sprout as described in claim 1, it is characterised in that: described in step 3
Processing that seed II carries out disinfection be；With 75% alcohol disinfecting 30s, aseptic water washing 3 times, then with 0.2% permanganic acid
Potassium sterilizes 30s, aseptic water washing 3 times, finally 10min is handled with 0.2% mercuric chloride, with aseptic water washing 5 times.
10. the sour processing method for promoting tuniclike psammosilene root seed to sprout as described in claim 1, it is characterised in that:
In step 4, condition of culture is: temperature is 25 ± 3 DEG C, relative humidity 70%-80%.
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| US20020124465A1 (en) * | 2001-03-08 | 2002-09-12 | Kharkwal Amit Chandra | Method for inducing improved seed germination in podophyllum hexandrum royle |
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