CN109231467B - Method for treating black and odorous water body by adopting gas lift circulation algae biofilm reactor - Google Patents
Method for treating black and odorous water body by adopting gas lift circulation algae biofilm reactor Download PDFInfo
- Publication number
- CN109231467B CN109231467B CN201811219244.3A CN201811219244A CN109231467B CN 109231467 B CN109231467 B CN 109231467B CN 201811219244 A CN201811219244 A CN 201811219244A CN 109231467 B CN109231467 B CN 109231467B
- Authority
- CN
- China
- Prior art keywords
- reactor
- black
- odorous water
- water body
- treating
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 title claims abstract description 83
- 238000000034 method Methods 0.000 title claims abstract description 48
- 241000195493 Cryptophyta Species 0.000 title claims abstract description 45
- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 claims abstract description 38
- 238000005273 aeration Methods 0.000 claims abstract description 25
- 235000007091 Chlorella pyrenoidosa Nutrition 0.000 claims abstract description 23
- OAICVXFJPJFONN-UHFFFAOYSA-N Phosphorus Chemical compound [P] OAICVXFJPJFONN-UHFFFAOYSA-N 0.000 claims abstract description 21
- 239000007789 gas Substances 0.000 claims abstract description 19
- 229910052757 nitrogen Inorganic materials 0.000 claims abstract description 19
- 229910052698 phosphorus Inorganic materials 0.000 claims abstract description 19
- 239000011574 phosphorus Substances 0.000 claims abstract description 19
- 239000010865 sewage Substances 0.000 claims abstract description 15
- 230000012010 growth Effects 0.000 claims abstract description 13
- 239000003344 environmental pollutant Substances 0.000 claims abstract description 12
- 231100000719 pollutant Toxicity 0.000 claims abstract description 12
- 238000006243 chemical reaction Methods 0.000 claims abstract description 10
- 238000003306 harvesting Methods 0.000 claims abstract description 9
- 239000012528 membrane Substances 0.000 claims abstract description 7
- 230000001174 ascending effect Effects 0.000 claims abstract description 6
- 238000012258 culturing Methods 0.000 claims abstract description 6
- 235000015097 nutrients Nutrition 0.000 claims abstract description 6
- 238000007599 discharging Methods 0.000 claims abstract description 5
- 241000195652 Auxenochlorella pyrenoidosa Species 0.000 claims abstract 9
- 235000016709 nutrition Nutrition 0.000 claims description 13
- 238000012163 sequencing technique Methods 0.000 claims description 9
- 238000005286 illumination Methods 0.000 claims description 7
- 230000014759 maintenance of location Effects 0.000 claims description 7
- 230000035764 nutrition Effects 0.000 claims description 6
- 239000008103 glucose Substances 0.000 claims description 4
- 238000012856 packing Methods 0.000 claims description 4
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 claims description 3
- 238000011010 flushing procedure Methods 0.000 claims description 3
- 239000011521 glass Substances 0.000 claims description 3
- 238000011081 inoculation Methods 0.000 claims description 3
- 241000894007 species Species 0.000 claims description 3
- 239000001963 growth medium Substances 0.000 claims description 2
- 230000008901 benefit Effects 0.000 abstract description 9
- 244000249214 Chlorella pyrenoidosa Species 0.000 description 14
- 239000002028 Biomass Substances 0.000 description 13
- 241000195649 Chlorella <Chlorellales> Species 0.000 description 10
- 238000005516 engineering process Methods 0.000 description 8
- 229910052799 carbon Inorganic materials 0.000 description 7
- OKTJSMMVPCPJKN-UHFFFAOYSA-N Carbon Chemical compound [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 description 6
- 230000000694 effects Effects 0.000 description 6
- 238000012546 transfer Methods 0.000 description 6
- CURLTUGMZLYLDI-UHFFFAOYSA-N Carbon dioxide Chemical compound O=C=O CURLTUGMZLYLDI-UHFFFAOYSA-N 0.000 description 4
- 239000000945 filler Substances 0.000 description 4
- 230000008569 process Effects 0.000 description 4
- 230000009286 beneficial effect Effects 0.000 description 3
- 238000010170 biological method Methods 0.000 description 3
- 238000011161 development Methods 0.000 description 3
- 230000018109 developmental process Effects 0.000 description 3
- 230000032770 biofilm formation Effects 0.000 description 2
- 230000015572 biosynthetic process Effects 0.000 description 2
- 229910002092 carbon dioxide Inorganic materials 0.000 description 2
- 239000001569 carbon dioxide Substances 0.000 description 2
- 238000010586 diagram Methods 0.000 description 2
- 230000007613 environmental effect Effects 0.000 description 2
- 239000000463 material Substances 0.000 description 2
- 238000001000 micrograph Methods 0.000 description 2
- 238000012986 modification Methods 0.000 description 2
- 230000004048 modification Effects 0.000 description 2
- 238000011160 research Methods 0.000 description 2
- 239000010802 sludge Substances 0.000 description 2
- 238000006467 substitution reaction Methods 0.000 description 2
- 239000002351 wastewater Substances 0.000 description 2
- 235000017166 Bambusa arundinacea Nutrition 0.000 description 1
- 235000017491 Bambusa tulda Nutrition 0.000 description 1
- 244000082204 Phyllostachys viridis Species 0.000 description 1
- 235000015334 Phyllostachys viridis Nutrition 0.000 description 1
- 238000010521 absorption reaction Methods 0.000 description 1
- 230000009471 action Effects 0.000 description 1
- 230000032683 aging Effects 0.000 description 1
- GZCGUPFRVQAUEE-SLPGGIOYSA-N aldehydo-D-glucose Chemical compound OC[C@@H](O)[C@@H](O)[C@H](O)[C@@H](O)C=O GZCGUPFRVQAUEE-SLPGGIOYSA-N 0.000 description 1
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 description 1
- 230000001651 autotrophic effect Effects 0.000 description 1
- 239000011425 bamboo Substances 0.000 description 1
- 238000006065 biodegradation reaction Methods 0.000 description 1
- 239000000969 carrier Substances 0.000 description 1
- 150000001875 compounds Chemical class 0.000 description 1
- 230000007547 defect Effects 0.000 description 1
- 230000009977 dual effect Effects 0.000 description 1
- 238000004134 energy conservation Methods 0.000 description 1
- 238000002474 experimental method Methods 0.000 description 1
- 230000002349 favourable effect Effects 0.000 description 1
- 239000003337 fertilizer Substances 0.000 description 1
- 239000002054 inoculum Substances 0.000 description 1
- 229910003471 inorganic composite material Inorganic materials 0.000 description 1
- 230000003834 intracellular effect Effects 0.000 description 1
- 230000007246 mechanism Effects 0.000 description 1
- 239000002609 medium Substances 0.000 description 1
- 230000002503 metabolic effect Effects 0.000 description 1
- 230000000813 microbial effect Effects 0.000 description 1
- 244000005700 microbiome Species 0.000 description 1
- 239000000203 mixture Substances 0.000 description 1
- 229910052760 oxygen Inorganic materials 0.000 description 1
- 239000001301 oxygen Substances 0.000 description 1
- 239000002957 persistent organic pollutant Substances 0.000 description 1
- 238000000053 physical method Methods 0.000 description 1
- 238000005728 strengthening Methods 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
- 239000002352 surface water Substances 0.000 description 1
- 230000001360 synchronised effect Effects 0.000 description 1
- 230000009105 vegetative growth Effects 0.000 description 1
- 238000004065 wastewater treatment Methods 0.000 description 1
Images
Classifications
-
- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F3/00—Biological treatment of water, waste water, or sewage
- C02F3/32—Biological treatment of water, waste water, or sewage characterised by the animals or plants used, e.g. algae
- C02F3/322—Biological treatment of water, waste water, or sewage characterised by the animals or plants used, e.g. algae use of algae
- C02F3/325—Biological treatment of water, waste water, or sewage characterised by the animals or plants used, e.g. algae use of algae as symbiotic combination of algae and bacteria
-
- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F3/00—Biological treatment of water, waste water, or sewage
- C02F3/34—Biological treatment of water, waste water, or sewage characterised by the microorganisms used
-
- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F2101/00—Nature of the contaminant
- C02F2101/10—Inorganic compounds
- C02F2101/105—Phosphorus compounds
-
- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F2101/00—Nature of the contaminant
- C02F2101/10—Inorganic compounds
- C02F2101/16—Nitrogen compounds, e.g. ammonia
-
- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F2101/00—Nature of the contaminant
- C02F2101/30—Organic compounds
-
- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F2303/00—Specific treatment goals
- C02F2303/02—Odour removal or prevention of malodour
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Microbiology (AREA)
- Biotechnology (AREA)
- Water Supply & Treatment (AREA)
- Biodiversity & Conservation Biology (AREA)
- Hydrology & Water Resources (AREA)
- Environmental & Geological Engineering (AREA)
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Botany (AREA)
- Ecology (AREA)
- Treating Waste Gases (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Abstract
The invention belongs to the technical field of sewage treatment, and discloses a method for treating black and odorous water by using a gas lift circulation algae biofilm reactor. Inoculating the chlorella pyrenoidosa to a biological rope carrier for culturing by hanging a membrane, leading the black and odorous water body into a column body of a reactor from a water inlet, then starting a light source and an aeration device, leading gas to rise and divide the reactor into an ascending area and a descending area through a guide cylinder, leading the biological rope carrier to be positioned in the ascending area, removing nitrogen and phosphorus pollutants in the black and odorous water body by the chlorella pyrenoidosa on the biological rope carrier through mixed nutrient growth reaction, then closing the light source and the aeration device for standing, and finally discharging the treated water from a water outlet. The device and the method have the advantages of low operation cost, good effluent quality, easy harvesting of algae and the like, and have great application potential in the treatment aspect of black and odorous water bodies.
Description
Technical Field
The invention belongs to the technical field of sewage treatment, and particularly relates to a method for treating black and odorous water by using a gas lift circulation algae biofilm reactor.
Background
In recent years, the blackening and odorization of urban water bodies are gradually increased, and the treatment of the black and odorous water bodies is urgent. Aiming at the problem that nitrogen, phosphorus and organic pollutants in the water body exceed standards, which is the root cause of the formation of black and odorous water body, the conventional treatment methods comprise a physical method, a chemical method and a biological method. The biological method has the advantages of environmental protection, ecological energy conservation and the like, and becomes the most development potential black and odorous water body treatment method. Although the removal rate of COD by the traditional biological method such as an activated sludge method and a new process developed on the basis can reach more than 90%, the nitrogen and phosphorus removal efficiency is generally low, and the requirement of 'upgrading' of sewage treatment is difficult to meet. The microalgae has the advantages of high growth rate, high economic value, good effect of removing nitrogen and phosphorus pollutants and the like, and has remarkable advantages in the field of eutrophic water body treatment.
Research results at home and abroad show that the chlorella (Chlorell) widely used for sewage treatmenta sp.) has the characteristics of high growth rate, strong environment adaptability and the like, and can utilize CO not only2The compound fertilizer can perform photoautotrophy, and can absorb organic carbon source, inorganic carbon source and light energy simultaneously to perform mixed vegetative growth. Under different culture conditions, the cell structure, the intracellular material composition and the physiological metabolic mechanism of chlorella are changed. A large number of researches show that compared with photoautotrophic Chlorella sp, the mixed nutrition Chlorella sp has a higher growth rate, a higher light energy utilization efficiency and a stronger nitrogen and phosphorus absorption capacity, and can absorb carbon dioxide in the atmosphere and light energy to continuously remove nitrogen and phosphorus pollutants in sewage under the condition that an organic carbon source is exhausted. Li et al (Li T., Zheng, Y., Yu, L.,&chen, s., Applied microbiology and biotechnology,2014.66,204-213), et al performed photoautotrophic and mixotrophic culture of chlorella, respectively, in a suspended microalgae wastewater treatment system, and the results showed that the specific growth rate and maximum biomass of chlorella were 5.4 and 5.2 times higher than those of the photoautotrophic mode, respectively, in the mixotrophic culture mode with 4g/L glucose added. Wang et al (Wang, h., Xiong, h., Hui, z.,&zeng, X., Bioresource Technology,2012.104,215-220.) mixed nutrient culture of Chlorella pyrenoidosa with pig wastewater diluted to different extents showed a COD of 1000 mg.L-1When the highest biomass concentration of 0.29 g.L was obtained-1The removal rates for COD, TN and TP were also the highest, respectively 55.4%, 74.6% and 77.7%. Therefore, the mixed nutrition type chlorella sewage treatment technology has higher pollutant removal rate and higher biomass yield.
However, the existing mixed nutrition type chlorella sewage treatment technology mostly adopts a suspended algae culture system, has the problems of high concentration of suspended matters in effluent, large floor area, difficult harvesting of algae biomass and the like, and the most effective solution is an algae immobilization technology. On the basis of microalgae immobilization, the algae biofilm is developed into a new sewage treatment means, the technology not only reduces the cost of a biofilm carrier, but also is simpler and more convenient in links such as algae inoculation, culture, harvest and the like. Feng et al (Gao, F., Yang, Z.H., Li, C.et al, Bioresource Technology,2015.179,8-12) utilize algal photobiofilm with chlorella biofilmThe reactor treats secondary sewage, NH4 +-N，PO4 3-The removal rate of P reaches 96 percent and 85.9 percent respectively, and the yield of the algae biomass is improved by 44 percent compared with the suspended algae reactor.
Typical algae biomembrane systems comprise a hydraulic algae bed, a biological rotating disc, a tubular and flat algae biomembrane photoreactor and the like, but the problems of large occupied area, unstable biomass, low mass transfer efficiency and the like generally exist, and the development of a high-efficiency bioreactor capable of being applied in engineering has very important significance for the development of algae sewage treatment technology. The columnar air-lift circulation photobioreactor not only has the advantages of small occupied area, high light energy utilization rate of algae and high mass transfer efficiency, but also can prevent the problem of supersaturation of dissolved oxygen in wastewater and improve the pollutant removal efficiency of the reactor. The invention discloses an airlift internal circulation combined filler bioreactor, which is disclosed in the Chinese patent with the publication number of CN 203639230U, and the invention provides that biological ceramsite filler and bamboo filament filler are respectively filled in an upflow zone and a downflow zone of the airlift circulation bioreactor, so that the airlift internal circulation combined filler bioreactor has good treatment effect on low-concentration, low-carbon and high-ammonia nitrogen sewage. However, the main body of the airlift internal circulation packing bioreactor which plays a role in biodegradation still is activated sludge, the dephosphorization effect of the reactor is poor, and the requirements of denitrification and dephosphorization of black and odorous water bodies cannot be met.
Disclosure of Invention
Aiming at the defects and shortcomings of the prior art, the invention aims to provide a method for treating black and odorous water by adopting an air lift circulation algae biofilm reactor. The method combines the dual characteristics of the mixed nutritional algae biomembrane technology and the columnar gas-lift circulation reactor, constructs a novel sequencing batch type gas-lift inner circulation mixed nutritional algae biomembrane nitrogen and phosphorus removal reaction system under the open culture condition, deeply removes nitrogen and phosphorus pollutants in the black and odorous water body so as to achieve the treatment target of the black and odorous water body, and simultaneously harvests the high-biomass microalgae.
The purpose of the invention is realized by the following technical scheme:
a method for treating black and odorous water by adopting a gas lift circulation algae biofilm reactor, wherein the gas lift circulation algae biofilm reactor comprises a light-transmitting cylinder, a light-transmitting guide cylinder is arranged in the cylinder, and a biological rope carrier is arranged in the guide cylinder; the lower end of the column body is provided with a conical bottom, and the center of the conical bottom is provided with an aeration device; the outer wall of the column body is wound with a white light LED lamp strip light source and is provided with a water inlet and a water outlet; the method for treating the black and odorous water body comprises the following steps:
inoculating Chlorella pyrenoidosa (Chlorella pyrenoidosa) to a biological rope carrier for culturing by hanging a membrane, introducing black and odorous water into a column body of a reactor from a water inlet, then starting a light source and an aeration device, enabling gas to rise and divide the reactor into an ascending region and a descending region through a guide cylinder, enabling the biological rope carrier to be located in the ascending region, removing nitrogen and phosphorus pollutants in the black and odorous water through mixed nutrient growth reaction of the Chlorella pyrenoidosa on the biological rope carrier, then closing the light source and the aeration device for standing, and finally discharging treated water from a water outlet.
Further, the chlorella pyrenoidosa inoculated on the biological rope carrier is added with 10 g.L-1The culture medium BG11 of glucose is obtained by aseptic preculture, and the inoculation amount of the chlorella pyrenoidosa is 20% of the effective volume of the reactor.
Further, the COD concentration of the black and odorous water body is 300-400 mg.L-1，NH4 +The concentration of-N is 17-20 mg.L-1，PO4 3-The concentration of-P is 8-10 mg.L-1。
Furthermore, the column body, the conical bottom and the guide cylinder are all made of organic glass, the height of the column body is 90cm, and the inner diameter of the column body is 9 cm; the height of the guide shell is 80cm, the diameter of the guide shell is 6cm, and the distance from the lower end of the guide shell to the center of the conical bottom of the reactor is 2 cm.
Further, the packing rate of the bio-rope carrier is 2/3 of the effective volume of the reactor. The biological rope carrier is an organic and inorganic composite material, has the advantages of microbial affinity, hydrophilicity, large specific surface area, rapid film formation, long service life of the material and low cost.
Further, the time for leading the black and odorous water body into the column body of the reactor from the water inlet is 1h, the time for starting the light source and the aeration device is 16h, the standing time is 6h, the time for discharging the treated water from the water outlet is 1h,one sequencing batch operation cycle is 24 hours, the light-dark ratio is 16 hours to 8 hours, and the aeration rate of the aeration device is 0.06 L.min-1The hydraulic retention time was 4 d.
Further, the total length of the light source of the white light LED lamp strip is 10m, the power is 5.8W, and the illumination intensity is 8000-10000 lux.
Further, the temperature of the mixed nutrition growth reaction is 25-28 ℃.
Further, the chlorella pyrenoidosa cultured by film hanging in the reactor is manually harvested by adopting a hydraulic flushing method every 7 days.
Furthermore, the reactor is also provided with a temperature controller and a microcomputer control system for controlling the temperature, the illumination intensity, the light-dark ratio, the water inlet and outlet time, the aeration rate and the hydraulic retention time parameters of the reactor.
The method of the invention has the following advantages and beneficial effects:
(1) the chlorella pyrenoidosa adopted by the method can simultaneously absorb an organic carbon source, an inorganic carbon source and light energy for growth, has a higher growth rate, a higher light energy utilization rate and a stronger environment adaptability compared with the chlorella pyrenoidosa autotrophic, can continuously absorb carbon dioxide and light energy in the atmosphere to remove nitrogen and phosphorus pollutants in sewage under the condition of depletion of the organic carbon source, takes the chlorella pyrenoidosa mixis as an algal species, and forms a mixed nutritional type phycomycete biomembrane under an open culture condition with a higher pollutant removal rate and a higher biomass yield.
(2) The method arranges the biological rope carrier in the upflow zone of the reactor, is beneficial to attaching algae to form an algae biofilm, and solves the problem of algae harvest.
(3) In the treatment process of the method, sewage is bent and ascended among the biological rope carriers under the action of the bottom aeration device, and flows downwards from the downflow zone when reaching the top of the guide cylinder, and the bottom of the guide cylinder is lifted again and is in contact reaction with the mixed nutritional algae biofilm again to form an internal circulation flow state. The flow state is favorable for strengthening the mass transfer process and improving the mass transfer efficiency of the reactor.
(4) The method can further adopt a temperature controller and a microcomputer control system to control a sequencing batch type operation mode, and can keep higher microalgae biomass in the reactor by controlling parameters such as temperature, illumination intensity, light-dark ratio, water inlet and outlet time, aeration rate, hydraulic retention time and the like of the reactor, thereby realizing synchronous nitrogen and phosphorus removal of the black and odorous water body, and the harvested microalgae can be recycled.
(5) The treatment method has the advantages of high mass transfer efficiency, good effluent quality, high microalgae yield, easy harvesting of the algae and the like, and has better application prospect in the aspect of removing nitrogen and phosphorus pollutants in eutrophic water body.
Drawings
FIG. 1 is a schematic structural diagram of a gas lift loop algae biofilm reactor in an embodiment of the invention.
FIG. 2 is a scanning electron microscope image of the surface of a biofilm obtained by biofilm culturing on a bio-rope carrier in an embodiment of the present invention.
FIG. 3 is a photograph (2600 times magnification) of a microscopic observation of biomass harvested in an example of the present invention.
FIG. 4 and FIG. 5 are views showing Nitrogen (NH) in the black and odorous water body according to the embodiment, respectively4 +-N) and Phosphorus (PO)4 3--P) water in and out concentration and removal rate as a function of days of operation.
Detailed Description
The present invention will be described in further detail with reference to examples and drawings, but the present invention is not limited thereto.
Examples
In the method for treating black and odorous water by using the gas lift circulation algae biofilm reactor, the structural schematic diagram of the gas lift circulation algae biofilm reactor is shown in fig. 1. Comprises a light-transmitting cylinder 1, a light-transmitting guide cylinder 2 is arranged in the cylinder, and a biological rope carrier 3 is arranged in the guide cylinder; the lower end of the column body is provided with a cone bottom 4, and the center of the cone bottom is provided with an aeration device 5; the outer wall of the column body is wound with a white light LED lamp strip light source 6 and is provided with a water inlet 7 and a water outlet 8; the aeration device 5 is connected with an air compressor 10 through a rotor flow meter 9; the reactor is also provided with a temperature controller 11 and a microcomputer control system 12 for controlling the parameters of the reactor, such as temperature, illumination intensity, light-dark ratio, water inlet and outlet time, aeration rate, hydraulic retention time and the like. The column body, the conical bottom and the guide cylinder are all made of organic glass, the height of the column body is 90cm, and the inner diameter of the column body is 9 cm; the height of the guide shell is 80cm, the diameter of the guide shell is 6cm, and the distance from the lower end of the guide shell to the center of the conical bottom of the reactor is 2 cm. The packing rate of the bio-rope carrier is 2/3 of the effective volume of the reactor. The total length of a light source of the white light LED lamp strip is 10m, the power is 5.8W, and the illumination intensity is 8000-10000 lux.
The method for treating the black and odorous water body comprises the following steps:
by adding 10 g.L-1Glucose BG11 medium sterile pre-cultured Chlorella pyrenoidosa (Chlorella pyrenoidosa) as an algal species was inoculated onto a bio-cord carrier for biofilm culturing, the inoculum size was 20% of the reactor effective volume. The black and odorous water body (COD concentration is 300-400 mg.L)-1，NH4 +The concentration of-N is 17-20 mg.L-1，PO4 3-The concentration of-P is 8-10 mg.L-1) The biological cord carrier is positioned in the upflow zone, the chlorella pyrenoidosa on the biological cord carrier removes nitrogen and phosphorus pollutants in the black and odorous water body through mixed nutrient growth reaction, then the light source and the aeration device are closed for standing, and finally the treated water is discharged from the water outlet. The temperature controller and the microcomputer control system are adopted to control the reactor to operate in a sequencing batch mode, the sequencing batch mode comprises the steps of water inlet for 1h, micro aeration for 16h, standing for 6h and water outlet for 1h, and the sequencing batch period is 24 h. Turning on the light source during aeration reaction, turning off the light source during water inlet, standing and water outlet, and controlling aeration rate at 0.06 L.min-1The hydraulic retention time is 4d, and the reaction temperature is controlled to be 25-28 ℃. By utilizing the characteristics of high growth rate, strong environment adaptability and the like of the mixed nutritional type phycomycete biomembrane, the characteristic that the sequencing batch operation mode can keep higher algae biomass and the advantage that the mass transfer efficiency of the loop reactor in the air lift is high, the nitrogen and phosphorus removal efficiency of the black and odorous water body is synchronously improved, and the manual harvesting of the phycomycete biomembrane is carried out in 7d of the experiment, so that the harvested microalgae can be recycledThe application is as follows.
In this example, the scanning electron microscope image of the surface of the biofilm obtained by inoculating pre-cultured chlorella pyrenoidosa to a bio-rope carrier and culturing the biofilm in a biofilm formation manner is shown in fig. 2. As can be seen from FIG. 2, a large number of microorganisms are attached to the biological rope carrier, the biofilm formation effect is good, the surface of the biofilm is uneven, and cells are directly connected with each other to form a thick mixed nutrition type phycomycete biofilm, which is beneficial to solving the problem of algae harvest.
When the reactor is operated to the 7 th day and the phenomenon of the aging of the biological membrane occurs, the cells in the reactor and on the carrier are manually harvested by the hydraulic flushing method, and the harvested biomass is observed by a microscope (magnified 2600 times), and the result is shown in FIG. 3. As can be seen from FIG. 3, Chlorella is the main strain in the reactor. The biomass of the algae harvested in the reactor was measured by dry weight method to a concentration of 0.67 g.L-1The yield was 0.096 g.L-1·d-1. The result shows that the treatment process can obtain higher microalgae productivity, and realizes the resource utilization of the algae while improving the pollution removal efficiency.
This example shows Nitrogen (NH) in black and odorous water4 +-N) and Phosphorus (PO)4 3-The inlet and outlet water concentrations and removal rates of-P) as a function of the number of days of operation are shown in FIGS. 4 and 5, respectively. As can be seen from the results of FIG. 4 and FIG. 5, the mixed nutrient type phycomycete biomembrane has good adaptability to black and odorous water body, and can achieve good and stable nitrogen and phosphorus removal effect at the 2 nd operation stage of the reactor, and NH during the stable operation stage4 +-N and PO4 3-The average removal rate of-P reaches 90.35 percent and 97.63 percent respectively, and the effluent NH is4 +-N and PO4 3-The concentration of-P can be respectively reduced to 2.0 mg.L-1And 0.2 mg. L-1The water quality standard meets the V-class water standard of the environmental quality standard of surface water (GB 3838-2002). Therefore, the air lift internal circulation algae biofilm reactor and the operation method thereof have good nitrogen and phosphorus removal effect on sewage and have great application potential in the aspect of black and odorous water body treatment.
The above embodiments are preferred embodiments of the present invention, but the present invention is not limited to the above embodiments, and any other changes, modifications, substitutions, combinations, and simplifications which do not depart from the spirit and principle of the present invention should be construed as equivalents thereof, and all such changes, modifications, substitutions, combinations, and simplifications are intended to be included in the scope of the present invention.
Claims (9)
1. A method for treating black and odorous water by adopting a gas lift circulation algae biofilm reactor is characterized by comprising the following steps: adopting a sequencing batch operation mode, treating sewage by using a mixed nutritional type chlorella pyrenoidosa biological membrane with the mixed nutritional type chlorella pyrenoidosa as an algal species under an open culture condition, and manually harvesting the chlorella pyrenoidosa cultured by hanging a membrane in a reactor every 7 days by adopting a hydraulic flushing method; the airlift circulation algae biomembrane reactor comprises a light-transmitting cylinder, wherein a light-transmitting guide cylinder is arranged in the cylinder, and a biological rope carrier is arranged in the guide cylinder; the lower end of the column body is provided with a conical bottom, and the center of the conical bottom is provided with an aeration device; the outer wall of the column body is wound with a white light LED lamp strip light source and is provided with a water inlet and a water outlet; the method for treating the black and odorous water body comprises the following steps: inoculating the chlorella pyrenoidosa to a biological rope carrier for culturing by hanging a membrane, leading the black and odorous water body into a column body of a reactor from a water inlet, then starting a light source and an aeration device, leading gas to rise and divide the reactor into an ascending area and a descending area through a guide cylinder, leading the biological rope carrier to be positioned in the ascending area, removing nitrogen and phosphorus pollutants in the black and odorous water body by the chlorella pyrenoidosa on the biological rope carrier through mixed nutrient growth reaction, then closing the light source and the aeration device for standing, and finally discharging the treated water from a water outlet.
2. The method for treating the black and odorous water body by using the gas lift circulation algae biofilm reactor of claim 1, wherein the method comprises the following steps: the chlorella pyrenoidosa inoculated on the biological rope carrier is additionally added with 10 g.L-1The culture medium BG11 of glucose is obtained by aseptic preculture, and the inoculation amount of the chlorella pyrenoidosa is 20% of the effective volume of the reactor.
3. The method of claim 1, wherein the algae is grown by air-lift circulationThe method for treating the black and odorous water body by the membrane reactor is characterized by comprising the following steps: the COD concentration of the black and odorous water body is 300-400 mg.L-1，NH4 +The concentration of-N is 17-20 mg.L-1，PO4 3-The concentration of-P is 8-10 mg.L-1。
4. The method for treating the black and odorous water body by using the gas lift circulation algae biofilm reactor of claim 1, wherein the method comprises the following steps: the column body, the conical bottom and the guide cylinder are all made of organic glass, the height of the column body is 90cm, and the inner diameter of the column body is 9 cm; the height of the guide shell is 80cm, the diameter of the guide shell is 6cm, and the distance from the lower end of the guide shell to the center of the conical bottom of the reactor is 2 cm.
5. The method for treating the black and odorous water body by using the gas lift circulation algae biofilm reactor of claim 1, wherein the method comprises the following steps: the packing rate of the bio-rope carrier is 2/3 of the effective volume of the reactor.
6. The method for treating the black and odorous water body by using the gas lift circulation algae biofilm reactor of claim 1, wherein the method comprises the following steps: the time for introducing the black and odorous water body into the column body of the reactor from the water inlet is 1h, the time for starting the light source and the aeration device is 16h, the standing time is 6h, the time for discharging the treated water from the water outlet is 1h, the sequencing batch operation period is 24h, the light-dark ratio is 16h:8h, and the aeration amount of the aeration device is 0.06 L.min-1The hydraulic retention time was 4 d.
7. The method for treating the black and odorous water body by using the gas lift circulation algae biofilm reactor of claim 1, wherein the method comprises the following steps: the total length of a light source of the white light LED lamp strip is 10m, the power is 5.8W, and the illumination intensity is 8000-10000 lux.
8. The method for treating the black and odorous water body by using the gas lift circulation algae biofilm reactor of claim 1, wherein the method comprises the following steps: the temperature of the mixed nutrition growth reaction is 25-28 ℃.
9. The method for treating the black and odorous water body by using the gas lift circulation algae biofilm reactor of claim 1, wherein the method comprises the following steps: the reactor is also provided with a temperature controller and a microcomputer control system for controlling the temperature, the illumination intensity, the light-dark ratio, the water inlet and outlet time, the aeration rate and the hydraulic retention time parameters of the reactor.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201811219244.3A CN109231467B (en) | 2018-10-19 | 2018-10-19 | Method for treating black and odorous water body by adopting gas lift circulation algae biofilm reactor |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201811219244.3A CN109231467B (en) | 2018-10-19 | 2018-10-19 | Method for treating black and odorous water body by adopting gas lift circulation algae biofilm reactor |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN109231467A CN109231467A (en) | 2019-01-18 |
| CN109231467B true CN109231467B (en) | 2022-03-01 |
Family
ID=65052423
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201811219244.3A Active CN109231467B (en) | 2018-10-19 | 2018-10-19 | Method for treating black and odorous water body by adopting gas lift circulation algae biofilm reactor |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN109231467B (en) |
Families Citing this family (10)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110395848A (en) * | 2019-08-05 | 2019-11-01 | 西北农林科技大学 | A method of black and odorous water is administered using microalgae |
| CN110723823A (en) * | 2019-10-25 | 2020-01-24 | 山东理工大学 | Device for jointly treating black and odorous water body by utilizing contact oxidation and microalgae |
| CN110723871B (en) * | 2019-10-29 | 2022-04-22 | 山东理工大学 | Method for treating black and odorous water by combining indigenous microbial film and microalgae |
| CN111533263A (en) * | 2020-05-12 | 2020-08-14 | 浙江伊诺环保科技股份有限公司 | Movable microecological treatment device and method for nitrogen and phosphorus removal of wastewater |
| CN112390381A (en) * | 2020-12-02 | 2021-02-23 | 灵珑生态科技(江苏)有限公司 | Device for quickly film-forming artificial aquatic weeds |
| CN112624527B (en) * | 2020-12-31 | 2022-09-23 | 哈尔滨工程大学烟台研究院 | Net algae-membrane biological composite sewage treatment device |
| CN112919641B (en) * | 2021-03-23 | 2024-01-16 | 南京科盛环保技术有限公司 | Method and treatment device for denitrification and dephosphorization by utilizing microalgae |
| CN113248021A (en) * | 2021-05-17 | 2021-08-13 | 哈尔滨工业大学 | Method for treating rural domestic sewage by using chlorella pyrenoidosa |
| CN113201439A (en) * | 2021-05-17 | 2021-08-03 | 江苏科技大学 | Chlorella culture device coupled with silk reeling wastewater treatment and culture method thereof |
| CN115784419A (en) * | 2022-12-13 | 2023-03-14 | 山东大学 | Organic carbon source supply method and system based on microalgae culture |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104830693A (en) * | 2015-05-20 | 2015-08-12 | 上海交通大学 | Nutrient switching method for culturing oleaginous microalgae |
| CN105692884A (en) * | 2016-03-18 | 2016-06-22 | 南开大学 | Aerobic granule sludge cultivation method based on helotism |
| CN105858899A (en) * | 2016-05-04 | 2016-08-17 | 湘潭大学 | Sewage deep treatment device based on fixed phycomycete system |
Family Cites Families (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| AU2015258329A1 (en) * | 2014-12-10 | 2016-06-30 | Chun Yean Chong | Microbial consortium for wastewater treatment |
-
2018
- 2018-10-19 CN CN201811219244.3A patent/CN109231467B/en active Active
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104830693A (en) * | 2015-05-20 | 2015-08-12 | 上海交通大学 | Nutrient switching method for culturing oleaginous microalgae |
| CN105692884A (en) * | 2016-03-18 | 2016-06-22 | 南开大学 | Aerobic granule sludge cultivation method based on helotism |
| CN105858899A (en) * | 2016-05-04 | 2016-08-17 | 湘潭大学 | Sewage deep treatment device based on fixed phycomycete system |
Also Published As
| Publication number | Publication date |
|---|---|
| CN109231467A (en) | 2019-01-18 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN109231467B (en) | Method for treating black and odorous water body by adopting gas lift circulation algae biofilm reactor | |
| JP4165715B2 (en) | Multiple photobioreactor and photosynthetic microorganism culture method using the same | |
| Wang et al. | Performance of different microalgae-based technologies in biogas slurry nutrient removal and biogas upgrading in response to various initial CO2 concentration and mixed light-emitting diode light wavelength treatments | |
| CN103910434B (en) | To exceed standard the membrane type bioreactor of nitrogen phosphorus and treatment process thereof for the treatment of natural pond liquid | |
| CN102392052A (en) | Biogas purification method by culturing autotrophic freshwater microalgae with biogas slurry | |
| CN105692884A (en) | Aerobic granule sludge cultivation method based on helotism | |
| CN112919641B (en) | Method and treatment device for denitrification and dephosphorization by utilizing microalgae | |
| CN110697884B (en) | Method for culturing bacteria-algae symbiotic granular sludge at low temperature | |
| CN102586075A (en) | Self-cleaning tubular photobioreactor | |
| CN106630483B (en) | Method for efficiently purifying biogas slurry based on algal-bacterial symbiosis | |
| CN104150586A (en) | Moving bed biofilm reactor and its sewage treatment method | |
| CN108623008A (en) | The method that microalgae-aerobic sludge granular syntaxial system is quickly formed under natural light | |
| CN108516618B (en) | Immobilized microalgae reactor with mycelium pellets as carrier and wastewater treatment method | |
| CN113105991B (en) | Micro-nano bubble culture and recovery integrated microalgae biofilm reactor and microalgae culture and recovery method thereof | |
| CN213865564U (en) | Utilize device of algae fungus biomembrane nitrogen and phosphorus removal | |
| CN107118982B (en) | Thiobacillus ferrooxidans and application thereof | |
| CN102211834A (en) | Light film symbiosis reaction device | |
| US20210380452A1 (en) | Method for treatment and resource utilization of acidic organic wastewater | |
| CN115028321B (en) | Process for purifying high ammonia nitrogen wastewater by coupling microalgae with short-range nitrification activated sludge | |
| CN109502914A (en) | A kind of sewage disposal system using chlorella processing biogas slurry | |
| CN108611276A (en) | Method for recycling vinasse wastewater by using microalgae | |
| CN113136342B (en) | Photo bioreactor, photosynthetic microorganism culture method and application thereof | |
| CN209383619U (en) | A kind of sewage disposal system using chlorella processing biogas slurry | |
| CN110885755A (en) | Method for polyculture culture of chlorella by using wheat starch processing wastewater | |
| CN103007741A (en) | Serial system light source control method for improving grade of methane by microalgae |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| TA01 | Transfer of patent application right | ||
| TA01 | Transfer of patent application right |
Effective date of registration: 20191111 Address after: 510640 Tianhe District, Guangdong, No. five road, No. 381, Applicant after: Zhu Bin Applicant after: Guangzhou South China University of Technology science and Technology Park Co., Ltd. Address before: 510640 Tianhe District, Guangdong, No. five road, No. 381, Applicant before: South China University of Technology |
|
| TA01 | Transfer of patent application right |
Effective date of registration: 20191127 Address after: Room 325 and 327, floor 3, block 22, Shunlian Machinery City, No. 18, Xingye 4th Road, Guanglong Industrial Park, Chihua neighborhood committee, Chencun Town, Shunde District, Foshan City, Guangdong Province Applicant after: Guangdong Zihua Technology Co., Ltd. Address before: 510640 Tianhe District, Guangdong, No. five road, No. 381, Applicant before: Zhu Bin Applicant before: Guangzhou South China University of Technology science and Technology Park Co., Ltd. |
|
| TA01 | Transfer of patent application right | ||
| TA01 | Transfer of patent application right | ||
| TA01 | Transfer of patent application right |
Effective date of registration: 20200611 Address after: 510640 Room 204, building 15, Xixiu village, Huagong, No. 381, Wushan Road, Tianhe District, Guangzhou City, Guangdong Province Applicant after: Li Ping Address before: Room 325 and 327, floor 3, block 22, Shunlian Machinery City, No. 18, Xingye 4th Road, Guanglong Industrial Park, Chihua neighborhood committee, Chencun Town, Shunde District, Foshan City, Guangdong Province Applicant before: Guangdong Zihua Technology Co.,Ltd. |
|
| CB03 | Change of inventor or designer information | ||
| CB03 | Change of inventor or designer information |
Inventor after: Li Ping Inventor after: Wu Jing Inventor after: Zhu Bin Inventor before: Zhu Bin Inventor before: Li Ping Inventor before: Wu Jing |
|
| GR01 | Patent grant | ||
| GR01 | Patent grant |