CN108516618B - Immobilized microalgae reactor with mycelium pellets as carrier and wastewater treatment method - Google Patents
Immobilized microalgae reactor with mycelium pellets as carrier and wastewater treatment method Download PDFInfo
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- 239000008188 pellet Substances 0.000 title claims abstract description 76
- 238000004065 wastewater treatment Methods 0.000 title claims abstract description 13
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- 238000000855 fermentation Methods 0.000 claims abstract description 18
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- 238000012258 culturing Methods 0.000 claims description 23
- 239000002351 wastewater Substances 0.000 claims description 23
- 238000011081 inoculation Methods 0.000 claims description 18
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- UIIMBOGNXHQVGW-UHFFFAOYSA-M Sodium bicarbonate Chemical compound [Na+].OC([O-])=O UIIMBOGNXHQVGW-UHFFFAOYSA-M 0.000 claims description 8
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- 239000000969 carrier Substances 0.000 claims description 6
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- 241000588748 Klebsiella Species 0.000 claims description 4
- 229910021380 Manganese Chloride Inorganic materials 0.000 claims description 4
- GLFNIEUTAYBVOC-UHFFFAOYSA-L Manganese chloride Chemical compound Cl[Mn]Cl GLFNIEUTAYBVOC-UHFFFAOYSA-L 0.000 claims description 4
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- ZPWVASYFFYYZEW-UHFFFAOYSA-L dipotassium hydrogen phosphate Chemical compound [K+].[K+].OP([O-])([O-])=O ZPWVASYFFYYZEW-UHFFFAOYSA-L 0.000 claims description 4
- 239000011790 ferrous sulphate Substances 0.000 claims description 4
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- 230000005484 gravity Effects 0.000 claims description 4
- BAUYGSIQEAFULO-UHFFFAOYSA-L iron(2+) sulfate (anhydrous) Chemical compound [Fe+2].[O-]S([O-])(=O)=O BAUYGSIQEAFULO-UHFFFAOYSA-L 0.000 claims description 4
- 229910000359 iron(II) sulfate Inorganic materials 0.000 claims description 4
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- PJNZPQUBCPKICU-UHFFFAOYSA-N phosphoric acid;potassium Chemical compound [K].OP(O)(O)=O PJNZPQUBCPKICU-UHFFFAOYSA-N 0.000 claims description 4
- 235000017557 sodium bicarbonate Nutrition 0.000 claims description 4
- 229910000030 sodium bicarbonate Inorganic materials 0.000 claims description 4
- 239000011684 sodium molybdate Substances 0.000 claims description 4
- 235000013619 trace mineral Nutrition 0.000 claims description 4
- 239000011573 trace mineral Substances 0.000 claims description 4
- NWONKYPBYAMBJT-UHFFFAOYSA-L zinc sulfate Chemical compound [Zn+2].[O-]S([O-])(=O)=O NWONKYPBYAMBJT-UHFFFAOYSA-L 0.000 claims description 4
- 229910000368 zinc sulfate Inorganic materials 0.000 claims description 4
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- 241000233866 Fungi Species 0.000 claims description 3
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- ARUVKPQLZAKDPS-UHFFFAOYSA-L copper(II) sulfate Chemical compound [Cu+2].[O-][S+2]([O-])([O-])[O-] ARUVKPQLZAKDPS-UHFFFAOYSA-L 0.000 claims 1
- 229910000366 copper(II) sulfate Inorganic materials 0.000 claims 1
- 230000015572 biosynthetic process Effects 0.000 abstract description 4
- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 description 40
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- 229910052757 nitrogen Inorganic materials 0.000 description 20
- 229910052698 phosphorus Inorganic materials 0.000 description 20
- 239000011574 phosphorus Substances 0.000 description 20
- 241000131448 Mycosphaerella Species 0.000 description 6
- 230000008901 benefit Effects 0.000 description 6
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- 238000005516 engineering process Methods 0.000 description 4
- 229910052927 chalcanthite Inorganic materials 0.000 description 3
- 230000007613 environmental effect Effects 0.000 description 3
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- 239000005416 organic matter Substances 0.000 description 2
- 239000010802 sludge Substances 0.000 description 2
- TVXXNOYZHKPKGW-UHFFFAOYSA-N sodium molybdate (anhydrous) Chemical compound [Na+].[Na+].[O-][Mo]([O-])(=O)=O TVXXNOYZHKPKGW-UHFFFAOYSA-N 0.000 description 2
- 230000031068 symbiosis, encompassing mutualism through parasitism Effects 0.000 description 2
- 150000003626 triacylglycerols Chemical class 0.000 description 2
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- 229910002092 carbon dioxide Inorganic materials 0.000 description 1
- 239000001569 carbon dioxide Substances 0.000 description 1
- 239000003153 chemical reaction reagent Substances 0.000 description 1
- 230000007547 defect Effects 0.000 description 1
- 239000010840 domestic wastewater Substances 0.000 description 1
- 239000003344 environmental pollutant Substances 0.000 description 1
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- 239000008187 granular material Substances 0.000 description 1
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- 239000010842 industrial wastewater Substances 0.000 description 1
- 244000005700 microbiome Species 0.000 description 1
- 239000010841 municipal wastewater Substances 0.000 description 1
- QMMRZOWCJAIUJA-UHFFFAOYSA-L nickel dichloride Chemical compound Cl[Ni]Cl QMMRZOWCJAIUJA-UHFFFAOYSA-L 0.000 description 1
- 238000010672 photosynthesis Methods 0.000 description 1
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- 238000002360 preparation method Methods 0.000 description 1
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- 102000004169 proteins and genes Human genes 0.000 description 1
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- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F3/00—Biological treatment of water, waste water, or sewage
- C02F3/32—Biological treatment of water, waste water, or sewage characterised by the animals or plants used, e.g. algae
- C02F3/322—Biological treatment of water, waste water, or sewage characterised by the animals or plants used, e.g. algae use of algae
- C02F3/325—Biological treatment of water, waste water, or sewage characterised by the animals or plants used, e.g. algae use of algae as symbiotic combination of algae and bacteria
-
- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F3/00—Biological treatment of water, waste water, or sewage
- C02F3/34—Biological treatment of water, waste water, or sewage characterised by the microorganisms used
- C02F3/341—Consortia of bacteria
-
- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F3/00—Biological treatment of water, waste water, or sewage
- C02F3/34—Biological treatment of water, waste water, or sewage characterised by the microorganisms used
- C02F3/347—Use of yeasts or fungi
-
- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F2209/00—Controlling or monitoring parameters in water treatment
- C02F2209/005—Processes using a programmable logic controller [PLC]
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- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F2209/00—Controlling or monitoring parameters in water treatment
- C02F2209/02—Temperature
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- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F2209/00—Controlling or monitoring parameters in water treatment
- C02F2209/06—Controlling or monitoring parameters in water treatment pH
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- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F2209/00—Controlling or monitoring parameters in water treatment
- C02F2209/22—O2
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- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F2305/00—Use of specific compounds during water treatment
- C02F2305/06—Nutrients for stimulating the growth of microorganisms
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- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Microbiology (AREA)
- Chemical & Material Sciences (AREA)
- Biotechnology (AREA)
- Biodiversity & Conservation Biology (AREA)
- Environmental & Geological Engineering (AREA)
- Water Supply & Treatment (AREA)
- Hydrology & Water Resources (AREA)
- Organic Chemistry (AREA)
- Mycology (AREA)
- Ecology (AREA)
- Botany (AREA)
- Purification Treatments By Anaerobic Or Anaerobic And Aerobic Bacteria Or Animals (AREA)
- Separation Of Suspended Particles By Flocculating Agents (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
Abstract
The invention discloses an immobilized microalgae reactor taking mycelium pellets as a carrier and a wastewater treatment method, wherein the reactor comprises a water inlet system, a reactor main body and a water outlet system, wherein the water inlet system comprises a water inlet tank, and the water inlet tank is sequentially connected with a water inlet pump and a water inlet pipe; the reactor main body comprises an ascending pipe and a descending pipe; the other end of the water inlet pipe is communicated with the top end of the downcomer, and the riser is positioned in the pipe cavity of the downcomer; the bottom of the down pipe is provided with an aeration head which is communicated with an air compressor through an air inlet pipe; the water outlet system comprises a water outlet tank and a water outlet pipe, and the water outlet pipe is arranged on the side wall of the descending pipe; the water outlet pipe is communicated with the down pipe, and water is discharged from the water outlet pipe to the water outlet tank. The method comprises the following steps: manufacturing a reactor; preparing concentrated algae solution, mycelium pellet suspension, flocculant fermentation liquor and photosynthetic bacteria concentrated solution; the bacteria-algae symbiotic system is started and operated quickly. The invention solves the problems of longer formation time and poorer stability of the mycelium pellet-microalgae symbiotic particle system.
Description
Technical Field
The invention belongs to the technical field of sewage biological treatment recycling and resource utilization, and particularly relates to an immobilized microalgae reactor using mycelium pellets as carriers and a wastewater treatment method.
Background
With the progress of industry and social development, municipal wastewater and water pollution discharged become more and more serious. Modern sewage treatment methods are mainly divided into physical treatment methods, chemical treatment methods, physical treatment methods and biological treatment methods. In contrast, biological wastewater treatment has been established as one of the main means for treating domestic wastewater, municipal mixed wastewater, and organic industrial wastewater for many years due to its wide application range and low investment and operation costs, and studies on wastewater treatment by algae in biological methods have been reported as early as the century. Microalgae is a photoautotrophic unicellular organism, which needs to consume nutrients such as nitrogen and phosphorus in water environment during growth to synthesize organic matter with physical energy, and CO is produced by photosynthesis2Fixed as organic carbon (protein, carbohydrate, oil), triacylglycerides in algal cell oils (Triacylglycerols,TAG) is a main raw material for preparing the biodiesel, some microalgae can also utilize an external carbon source for heterotrophic growth, and the growth amount and the grease amount are both greatly improved. In recent years, microalgae receive more and more attention in the aspects of biodiesel preparation and sewage deep nitrogen and phosphorus removal, and a possible solution is provided for relieving the fossil energy crisis and the water quality crisis faced by human in the 21 st century. The microalgae-based sewage treatment and high-value biological energy production coupled system technology takes organic wastewater nutrient substances as resources, can realize the conversion of a sewage treatment system from a treatment process to a production process, and has wider development prospect in a severe form of increasingly tense future energy and resources by culturing microalgae with nutrient substances such as nitrogen and phosphorus in sewage while deeply treating sewage to obtain high-value biological energy and biological resources. The activated sludge process is the most widely applied biological sewage treatment technology in the world at present, and the greatest defect of the technology is that a large amount of excess sludge is generated, and secondary pollution is easily caused to the environment. The mycelium pellet as a novel biomass carrier for fixing microorganisms provides a brand new method and thought for solving the problem. The mycelial pellets and the microalgae are cultured together to form a symbiotic system, so that the advantages of the mycelial pellets and the microalgae are exerted, a new mycelial and algal symbiotic system is expected to be formed, and a new method and a new idea are provided for a sewage treatment technology. But the problems of long formation time, poor stability and the like of the mycelium pellet-microalgae symbiotic particle system also limit the large-scale application of the mycelium pellet-microalgae symbiotic particle system in the field of water treatment.
Disclosure of Invention
In view of the above, the present invention provides an immobilized microalgae reactor using mycelium pellets as a carrier and a wastewater treatment method, which are directed to the problems of poor start-up and stable operation performance, poor sewage treatment effect, poor microalgae settling performance and difficult harvesting of bioreactors in the prior art.
In order to solve the technical problem, the invention discloses an immobilized microalgae reactor taking mycelium pellets as a carrier, which comprises a water inlet system, a reactor main body and a water outlet system, wherein the water inlet system comprises a water inlet tank, and the water inlet tank is sequentially connected with a water inlet pump and a water inlet pipe through a pipeline; the reactor main body comprises an ascending pipe and a descending pipe; the other end of the water inlet pipe is communicated with the top end of the downcomer, the riser is positioned in the pipe cavity of the downcomer, and the riser and the downcomer are coaxially sleeved from inside to outside; the bottom of the downcomer is provided with an aeration head which is communicated with an air compressor through an air inlet pipe; the water outlet system comprises a water outlet tank and a water outlet pipe, and the water outlet pipe is arranged on the side wall of the descending pipe; the water outlet pipe is communicated with the down pipe, and water is discharged from the water outlet pipe to the water outlet tank.
Optionally, the water outlet system further comprises a water outlet electromagnetic valve, the water outlet electromagnetic valve is arranged on the water outlet pipe and communicated with the water outlet pipe, the side wall of the downcomer is further provided with uniformly distributed sampling ports, and the sampling ports are arranged opposite to the water outlet pipe.
Optionally, the water outlet pipe is arranged at the height of 1/2-2/3 from the bottom of the riser, and the ratio of the height of the riser to the inner diameter of the riser is 1: 4-6; the ratio of the height of the downcomer to the inner diameter of the downcomer is 1: 4-7.
Optionally, the reactor further comprises a PLC controller, and the PLC controller is respectively connected with the water inlet pump, the electromagnetic water outlet valve, the air compressor, the external light source, the temperature controller, the dissolved oxygen sensor and the pH sensor through leads; the external light source is arranged on an external light source bracket, and the external light source bracket is arranged on the outer side wall of the descending tube.
Optionally, a reactor cover is arranged on the top of the downcomer and is provided with fixing holes for fixing the temperature controller, the dissolved oxygen sensor, the pH sensor and the water inlet pipe.
The invention also discloses a wastewater treatment method based on the immobilized microalgae reactor taking the mycelium pellet as the carrier, which comprises the following steps:
and 3, quickly starting and operating the bacteria-algae symbiotic system.
Optionally, the mycelium pellet suspension in step 2 comprises a fungal spore suspension, a mycelium pellet suspension or a broken mycelium suspension;
the fungal spore suspension is prepared by the following method: transferring the fungal spores on the inclined plane into sterile water containing glass beads, suspending the spores on the inclined plane in the water, placing the suspension containing the glass beads in a shaking table at 180rpm, and shaking for 2h to fully disperse the spores and ensure that each milliliter contains 10 spores8-109cfu; the method is used for culturing the bacteria-algae coupling system, and the inoculation amount of spore suspension is determined to be 1mL/1000mL of bacteria-algae coupling culture medium;
the mycelial ball spore is fungus Aspergillus niger;
the mycelium pellet suspension is prepared by the following method: the concentration of inoculated spores was 104M L, culturing the mycelium pellet on a shaker for 3 days at 37 ℃ and 160rpm, wherein the initial p H of a culture medium is 6-7, and the mycelium pellet culture medium is a bacteria-algae coupling culture medium;
the broken mycelium suspension was prepared by the following method: transferring the cultured mature mycelium pellets into sterile water filled with glass beads, placing the sterile water in a shaking table at 180rpm, shaking for 30min, crushing for 30s by using a stirrer, and inoculating the obtained mycelium fragments into a mycelial algae coupling culture medium to finish the process of culturing the mycelium pellets;
the concentrated algae solution is prepared by the following steps: inoculating microalgae into high-density culture microalgae shake flask, controlling temperature within 28-32 deg.C, culturing at 30 deg.C as optimum illumination, and culturing in the simulated food wastewater with illumination intensity of 5000Lux, pH of 7.0 and culture time of 96-120 hr. Culturing under the above suitable conditions to reach cell density of 109-1010The microalgae is finally transferred into a reactor, and the inoculation amount is 100mL/1000mL of the coupled system culture medium;
the bacteria-algae coupling culture medium is simulated food wastewater, and the formula of the bacteria-algae coupling culture medium is as follows: 1500.0mg/L glucose, 75.0mg/L beef extract, 112.5mg/L peptone, 300.0mg/L ammonium chloride, 30.0mg/L ferrous sulfate, 150.0mg/L anhydrous calcium chloride, 52.5mg/L dipotassium hydrogen phosphate, 22.5mg/L anhydrous potassium dihydrogen phosphate, 22.5mg/L magnesium sulfate, 1mL/L trace elements and a plurality of sodium bicarbonate;
wherein the microelement is H3BO4150mg/L，ZnSO4·7H2O 120mg/L，MnCl2·7H2O 120mg/L，CuSO4·5H2O 30mg/L，NiCl250mg/L，CoCl2·6H2O 210 mg/L，KI 30mg/L，Na2MoO4 65mg/L；
The bioflocculant fermentation liquor is prepared by fermentation of Klebsiella; the photosynthetic bacteria concentrated solution is zymocyte solution of rhodopseudomonas bacteria.
Optionally, the fast start and operation of the fungus-algae symbiotic system in step 3 specifically comprises:
step 3.1, water inflow: in each reaction period, the inlet water is kept at 20-30 ℃, the inlet pump leads the simulated wastewater in the inlet tank into the descending pipe of the reactor main body through the inlet pipe, the inlet time of the inlet pump is set to be 3-6 minutes through the PLC, and the inlet pump is closed after the inlet water is finished;
step 3.2, forming a coupling particle system: inoculating a certain amount of concentrated algae liquid and one of fungal spore suspension, mycelial ball suspension or broken mycelium suspension at an opening at the upper end of a reactor main body, simultaneously adding a certain amount of biological flocculant and photosynthetic bacteria concentrated liquid, starting an air compressor, supplying compressed air into the reactor main body from an aeration head through an air inlet pipe, enabling wastewater in the reactor main body to move upwards in an ascending pipe under the driving of ascending air flow, ascending to a joint of the ascending pipe and the upper end of a descending pipe, starting to enter the descending pipe, and then moving downwards to the bottom of the reactor; the fungal spores are aerated for 12 to 60 hours to gradually form mycelium pellets, the mycelium is intertwined in the pellet forming process, and the microalgae are tightly combined on the mycelium to form green mycelium pellets;
step 3.3, stabilizing operation: the bacteria and algae in the step 2 grow together, after bacteria and algae coupling particles are formed after a period of culture, an air compressor is closed to separate the bacteria and algae symbiont from sewage under the gravity condition, the bacteria and algae symbiont is precipitated for 1 minute, water is drained for 5 minutes, and then the reactor enters an idle period of 120 minutes; starting to enter a stable operation period, the reactor adopts intermittent water feeding
Optionally, the volume ratio of the addition amount of the fungal spore suspension in the step 3.2 to the reactor sewage is 1: 1000; the final inoculation concentration of the mycelium pellet suspension is 400mg/L, and the final inoculation concentration of the broken mycelium suspension is 200 mg/L; the volume ratio of the addition amount of the concentrated algae liquid to the sewage of the reactor is 1: 10; the volume ratio of the bioflocculant fermentation liquor to the reactor sewage is 1:50-1: 150; the volume ratio of the photosynthetic bacteria concentrated solution to the reactor sewage is 1:50-1: 150; the culture conditions for the coupled particle system were: the temperature is 20-30 ℃, the air ventilation amount is 60-180L/h, the dissolved oxygen is 3.0mg/L, pH6-8, the illumination intensity is 5000-.
Optionally, the operating conditions during steady operation in step 3 are as follows: the period is 12h, including water feeding in 5min, aeration (120L/h) for 589min, precipitation for 1min, water draining for 5min and standing for 120 min.
Compared with the prior art, the invention can obtain the following technical effects:
1) the reactor provided by the invention increases the contact mixing effect of sewage, algae and fungi in the water inlet stage of the reactor, and provides a foundation for realizing high-efficiency synchronous removal of nitrogen and phosphorus in a single-stage reactor; the reactor is simple and convenient to operate, the problem that economic microalgae of a microalgae biological treatment system is difficult to harvest is solved at room temperature, and the harvesting cost is greatly reduced;
2) according to the invention, the coupling system is formed in a mode of co-feeding spore suspension and microalgae, compared with the traditional mode of directly feeding the coupling system, the method saves the culture cost, saves the domestication time, improves the treatment efficiency of the whole system, prolongs the efficient and stable operation period, and has high pollutant removal efficiency and stable effluent quality; the reactor of the invention has the characteristics of low operating cost, small occupied area and the like; solves the problems of long formation time, poor stability and the like of a mycelial sphere-microalgae symbiotic particle system, and develops a method for fast formation, directional regulation and high-efficiency stable operation of mycelial and microalgae symbiosis.
3) The method has good synchronous carbon, nitrogen and phosphorus removal effect under the normal temperature condition, and the removal efficiency of carbon, nitrogen and phosphorus is 89.5%, 98.9% and 95.5% respectively.
4) The invention provides an effective method for controlling the emission of nitrogen and phosphorus in the normal-temperature organic wastewater, can be widely applied to the synchronous removal of nitrogen and phosphorus in the normal-temperature organic wastewater, and has good social, economic and environmental benefits.
Of course, it is not necessary for any one product in which the invention is practiced to achieve all of the above-described technical effects simultaneously.
Drawings
The accompanying drawings, which are included to provide a further understanding of the invention and are incorporated in and constitute a part of this specification, illustrate embodiments of the invention and together with the description serve to explain the invention and not to limit the invention. In the drawings:
FIG. 1 is a schematic diagram of the overall structure of an immobilized microalgae reactor using mycelium pellets as carriers according to the present invention;
FIG. 2 is a cross-sectional view of an immobilized microalgae reactor using mycelium pellets as carriers according to the present invention;
FIG. 3 is a schematic diagram of the connections between the PLC controller and other components of the present invention;
FIG. 4 is a comparison of a mature phycobiont granule system of the present invention with mycelial pellets; wherein, a represents a blank mycelial ball with white color, b represents a mycorrhizal symbiotic particle with green color;
wherein, 1, a water inlet tank; 2. a water inlet pump; 3. a water outlet electromagnetic valve; 4. a water outlet tank; 5. a riser pipe; 6. A down pipe; 7. an air compressor; 8. an aeration head; 9. a PLC controller; 11. a water inlet pipe; 12. a water outlet pipe; 13. an air inlet pipe; 14. a sampling port; 15. a temperature controller; 16. a dissolved oxygen sensor; 17. A pH sensor; 19. an external light source bracket; 20. external light source, 21, reactor cover.
Detailed Description
The following embodiments are described in detail with reference to the accompanying drawings, so that how to implement the technical features of the present invention to solve the technical problems and achieve the technical effects can be fully understood and implemented.
The invention discloses an immobilized microalgae reactor taking mycelium pellets as a carrier, which comprises a water inlet system, a reactor main body and a water outlet system as shown in figures 1 and 2, wherein the water inlet system comprises a water inlet tank 1, and the water inlet tank 1 is sequentially connected with a water inlet pump 2 and a water inlet pipe 11 through a pipeline;
the reactor main body comprises an ascending pipe 5 and a descending pipe 6; the other end of the water inlet pipe 11 is communicated with the top end of the downcomer 6, the ascending pipe 5 is positioned in the pipe cavity of the downcomer 6, and the ascending pipe 5 and the downcomer 6 are coaxially sleeved from inside to outside; an aeration head 8 is arranged at the bottom of the downcomer 6, and the aeration head 8 is communicated with an air compressor 7 through an air inlet pipe 13;
the water outlet system comprises a water outlet tank 4 and a water outlet pipe 12, and the water outlet pipe 12 is arranged on the side wall of the descending pipe 5; the water outlet pipe 12 is communicated with the down pipe 5, and the water outlet pipe 12 discharges water to the water outlet tank 4.
Optionally, the water outlet system further includes a water outlet solenoid valve 3, and the water outlet solenoid valve 3 is disposed on the water outlet pipe 12 and is communicated with the water outlet pipe 12.
Alternatively, the outlet pipe 12 is installed at a height of 1/2-2/3 from the bottom of the riser pipe 5, and the drainage ratio is controlled to be 40-60%.
Optionally, the sidewall of the downcomer 6 is further provided with evenly distributed sampling ports 14, and the sampling ports 14 are opposite to the outlet pipe 12, so that the arrangement positions are not overlapped, and mutual interference can be eliminated.
Optionally, the ratio of the height of the ascending pipe 5 to the inner diameter of the ascending pipe 5 is 1: 4-6; the ratio of the height of the downcomer 6 to the inner diameter of the downcomer 6 is 1: 4-7; so set up, increased reactor operation's stability.
Preferably, the ratio of the height of the ascending pipe 5 to the inner diameter of the ascending pipe is 1: 5.625; the ratio of the height of the downcomer 6 to the inner diameter of the downcomer is 1:5.25, and in the ratio range, the occupied area is saved, a certain height is met, sufficient contact time and hydraulic shearing force are provided, and hydraulic retention time is guaranteed.
Optionally, the reactor further comprises a PLC controller 9, as shown in fig. 3, the PLC controller 9 is connected to the water inlet pump 2, the electromagnetic water outlet valve 3, the air compressor 7, the external light source 20, the temperature controller 15, the dissolved oxygen sensor 16 and the pH sensor 17 through wires; the external light source 20 is arranged on the external light source bracket 19, and the external light source bracket 19 is arranged on the outer side wall of the descending tube 6.
The PLC 9 is used for actually controlling the power supply condition, the switch of one instrument is mastered through power supply, and the other function is used for monitoring certain parameter indexes of the reactor in real time through signals of the sensor; the temperature controller 15 is used for controlling the temperature change of water in the reactor and monitoring the water temperature; the dissolved oxygen sensor 16 is mainly used for monitoring the content of dissolved oxygen in the reactor; the pH sensor 17 is mainly to monitor the change in internal pH. All power supply control is completed through the PLC.
Optionally, a reactor cover 21 is disposed on the top of the downcomer 6, and the reactor cover 21 is provided with fixing holes for fixing the temperature controller 15, the dissolved oxygen sensor 16, the pH sensor 17, and the water inlet pipe 11.
The reactor can ensure that mycelium pellets and energy microalgae can grow and reproduce well under aerobic conditions, stable nitrogen and phosphorus removal and organic matter removal effects are achieved, the mycelium pellets are used as carriers, the problems that the competitiveness of the energy microalgae in a system is weak, the nitrogen and phosphorus removal effects are poor, dominant algae species are difficult to form, a large amount of algae are lost, and the stable operation period of the whole system is short can be solved, in addition, the problem of energy microalgae loss is effectively solved, and the mycelium pellets adsorbing the microalgae can be harvested through a simple filtering method. The mycelium pellet-microalgae coupling system formed by the invention exerts respective advantages of fungi, microalgae and bacteria, and realizes synergistic symbiosis, and the reactor and the method have the advantages of short system forming time, high stability, low cost, good effect of fixing carbon dioxide in air, high content of recovered microalgae grease, good sewage treatment effect of the coupling system, avoidance of use of chemical reagents, and ecological friendliness. The invention provides an effective method for controlling the discharge of nitrogen and phosphorus in organic wastewater, is widely applied to the synchronous removal of nitrogen and phosphorus in organic wastewater, and has good environmental benefit.
The invention also discloses a wastewater treatment method based on the immobilized microalgae reactor taking the mycelium pellet as the carrier, which comprises the following steps:
the mycelium pellet suspension comprises fungal spore suspension, mycelium pellet suspension or broken mycelium suspension;
the fungal spore suspension is prepared by the following method: transferring the fungal spores on the inclined plane into sterile water containing glass beads, suspending the spores on the inclined plane in the water, placing the suspension containing the glass beads in a shaking table at 180rpm, and shaking for 2h to fully disperse the spores and ensure that each milliliter contains 10 spores8-109cfu; the method is used for culturing the bacteria-algae coupling system, and the inoculation amount of spore suspension is determined to be 1mL/1000mL of bacteria-algae coupling culture medium;
the mycelium pellet suspension is prepared by the following method: the concentration of inoculated spores was 104M L, culturing the mycelium pellet on a shaker for 3 days at 37 ℃ and 160rpm, wherein the initial p H of a culture medium is 6-7, and the mycelium pellet culture medium is a bacteria-algae coupling culture medium;
the mycelial ball spore is fungus Aspergillus niger;
the broken mycelium suspension was prepared by the following method: transferring the cultured mature mycelium pellets into sterile water filled with glass beads, placing the sterile water in a shaking table at 180rpm, shaking for 30min, crushing for 30s by using a stirrer, and inoculating the obtained mycelium fragments into a mycelial algae coupling culture medium to finish the process of culturing the mycelium pellets;
the concentrated algae solution is prepared by the following steps: inoculating microalgae into high-density culture microalgae shake flask, controlling temperature within 28-32 deg.C, culturing at 30 deg.C as optimum illumination, and culturing in the simulated food wastewater with illumination intensity of 5000Lux, pH of 7.0 and culture time of 96-120 hr. Culturing under the above suitable conditions to reach cell density of 109-1010The microalgae is finally transferred into a reactor, and the inoculation amount is 100mL/1000mL of the coupled system culture medium;
the bacteria-algae coupling culture medium is simulated food wastewater, and the formula of the bacteria-algae coupling culture medium is as follows: 1500.0mg/L glucose, 75.0mg/L beef extract, 112.5mg/L peptone, 300.0mg/L ammonium chloride, 30.0mg/L ferrous sulfate, 150.0mg/L anhydrous calcium chloride, 52.5mg/L dipotassium hydrogen phosphate, 22.5mg/L anhydrous potassium dihydrogen phosphate, 22.5mg/L magnesium sulfate, 1mL/L trace elements and a plurality of sodium bicarbonate;
wherein the microelement is H3BO4 150mg/L，ZnSO4·7H2O 120mg/L，MnCl2·7H2O 120mg/L，CuSO4·5H2O 30mg/L，NiCl2 50mg/L，CoCl2·6H2O 210 mg/L，KI 30mg/L，Na2MoO4 65mg/L；
The bioflocculant fermentation liquor is prepared by fermentation of Klebsiella; the photosynthetic bacteria concentrated solution is zymocyte solution of rhodopseudomonas bacteria.
And step 3: the fast start and operation of the fungus-algae symbiotic system:
step 3.1, water inflow: in each reaction period, the inlet water is kept at 20-30 ℃, the inlet pump 2 pours the simulated sewage in the inlet tank 1 into the downcomer 6 of the reactor body through the inlet pipe 11, the inlet time of the inlet pump 2 is set to be 3-6 minutes through the PLC 9, and the inlet pump 2 is closed after the inlet water is finished;
step 3.2, forming a coupling particle system: inoculating a certain amount of one of a fungal spore suspension, a mycelial ball suspension or a broken mycelial suspension and a concentrated algae solution at an opening at the upper end of the reactor main body (the top of the downcomer 6), and simultaneously adding a certain amount of a biological flocculant and a photosynthetic bacteria concentrated solution, wherein the volume ratio of the addition amount of the fungal spore suspension to the reactor sewage is 1: 1000; the final inoculation concentration of the mycelium pellet suspension is 400mg/L, the final inoculation concentration of the broken mycelium suspension is 200mg/L, and the volume ratio of the addition amount of the concentrated algae liquid to the sewage of the reactor is 1: 10; simultaneously adding a certain amount of bioflocculant fermentation liquor and photosynthetic bacteria concentrated liquor, wherein the volume ratio of the bioflocculant fermentation liquor to the reactor sewage is 1:50-1: 150; the volume ratio of the photosynthetic bacteria concentrated solution to the reactor sewage is 1:50-1: 150; at the moment, an air compressor 7 is started, compressed air is supplied into the reactor main body from an aeration head 8 through an air inlet pipe 13, sewage in the reactor main body moves upwards in the ascending pipe 5 under the driving of ascending air flow, the sewage rises to the joint of the upper end of the ascending pipe 5 and the upper end of the descending pipe 6, starts to enter the descending pipe 6, and then moves downwards to the bottom of the reactor main body; the fungal spores are aerated for 12 to 60 hours to gradually form mycelium pellets, the mycelium is intertwined in the pellet forming process, and the microalgae are tightly combined on the mycelium to form green mycelium pellets; the culture conditions were as follows: the temperature is 20-30 ℃, the air ventilation capacity is 60-180L/h, the dissolved oxygen is 3.0mg/L, pH6-8, the illumination intensity is 5000-;
as shown in fig. 4, the green mycelial pellets outside the mycelial symbiotic particle system are green, and the whole mycelial pellets are proved to contain a large amount of microalgae.
Step 3.3, stabilizing operation: the bacteria and algae in the step 3.2 grow together, after bacteria and algae coupling particles are formed after a period of culture, the air compressor 7 is closed to separate the bacteria and algae symbiont from sewage under the gravity condition, the bacteria and algae symbiont is precipitated for 1 minute, water is drained for 5 minutes, and then the reactor enters an idle period of 120 minutes; in the stable operation period, the reactor adopts intermittent water feeding, and the operation conditions are as follows: the period is 12h, including water feeding in 5min, aeration for 589min (120L/h), precipitation for 1min, draining for 5min and idling for 120 min; the volume exchange rate of the reactor is 50 percent, and the hydraulic retention time is 12 hours.
The method of the invention provides an effective method for controlling the discharge of nitrogen and phosphorus in simulated wastewater (food wastewater), is widely applied to the synchronous removal of nitrogen and phosphorus in food wastewater, and has good environmental benefit.
Example 1
the reactor main body comprises an ascending pipe 5 and a descending pipe 6; the other end of the water inlet pipe 11 is communicated with the top end of a downcomer 6, the downcomer 6 is positioned in a pipe cavity of an ascending pipe 5, and the ascending pipe 5 and the downcomer 6 are coaxially sleeved from inside to outside; an aeration head 8 is arranged at the bottom of the downcomer 6, and the aeration head 8 is communicated with an air compressor 7 through an air inlet pipe 13;
the water outlet system comprises a water outlet tank 4 and a water outlet pipe 12, and the water outlet pipe 12 is arranged on the side wall of the descending pipe 5; the water outlet pipe 12 is communicated with the down pipe 5, and the water outlet pipe 12 discharges water to the water outlet tank 4.
Alternatively, the bottom of said downcomer 6 communicates with the bottom of the riser 5 and the top of the downcomer 6 communicates with the top of the riser 5.
Optionally, the water outlet system further includes a water outlet solenoid valve 3, and the water outlet solenoid valve 3 is disposed on the water outlet pipe 12 and is communicated with the water outlet pipe 12.
Alternatively, the outlet pipe 12 is installed at a height of 1/2-2/3 from the bottom of the riser pipe 5, and the drainage ratio is controlled to be 40-60%.
Optionally, the sidewall of the downcomer 6 is further provided with evenly distributed sampling ports 14, and the sampling ports 14 are opposite to the outlet pipe 12.
Optionally, the ratio of the height of the ascending pipe 5 to the inner diameter of the ascending pipe 5 is 1: 4-6; the ratio of the height of the downcomer 6 to the inner diameter of the downcomer 6 is 1: 4-7.
Preferably, the ratio of the height of the ascending pipe 5 to the inner diameter of the ascending pipe 5 is 1: 5.625; the ratio of the height of the downcomer 6 to the inner diameter of the downcomer 6 is 1: 5.25; so set up, increased reactor operation's stability.
Optionally, the reactor further comprises a PLC controller 9, and the PLC controller 9 is connected with the water inlet pump 2, the electromagnetic water outlet valve 3, the air compressor 7, the external light source 20, the temperature controller 15, the dissolved oxygen sensor 16 and the pH sensor 17 through wires; the external light source 20 is arranged on the external light source bracket 19, and the external light source bracket 19 is arranged on the outer side wall of the descending tube 6.
the mycelium pellet suspension comprises fungal spore suspension, mycelium pellet suspension or broken mycelium suspension;
the fungal spore suspension is prepared byThe method comprises the following steps: transferring the fungal spores on the inclined plane into sterile water containing glass beads, suspending the spores on the inclined plane in the water, placing the suspension containing the glass beads in a shaking table at 180rpm, and shaking for 2h to fully disperse the spores and ensure that each milliliter contains 10 spores8-109cfu; the method is used for culturing the bacteria-algae coupling system, and the inoculation amount of spore suspension is determined to be 1mL/1000mL of bacteria-algae coupling culture medium;
the mycelium pellet suspension is prepared by the following method: the concentration of inoculated spores was 104M L, culturing the mycelium pellet on a shaker for 3 days at 37 ℃ and 160rpm, wherein the initial p H of a culture medium is 6-7, and the mycelium pellet culture medium is a bacteria-algae coupling culture medium;
the mycelial ball spore is fungus Aspergillus niger;
the broken mycelium suspension was prepared by the following method: transferring the cultured mature mycelium pellets into sterile water filled with glass beads, placing the sterile water in a shaking table at 180rpm, shaking for 30min, crushing for 30s by using a stirrer, and inoculating the obtained mycelium fragments into a mycelial algae coupling culture medium to finish the process of culturing the mycelium pellets;
the concentrated algae solution is prepared by the following steps: inoculating microalgae into high-density culture microalgae shake flask, controlling temperature within 28-32 deg.C, culturing at 30 deg.C as optimum illumination, and culturing in the simulated food wastewater with illumination intensity of 5000Lux, pH of 7.0 and culture time of 96-120 hr. Culturing under the above suitable conditions to reach cell density of 109-1010The microalgae is finally transferred into a reactor, and the inoculation amount is 100mL/1000mL of the coupled system culture medium;
the bacteria-algae coupling culture medium is simulated food wastewater, and the formula of the bacteria-algae coupling culture medium is as follows: 1500.0mg/L glucose, 75.0mg/L beef extract, 112.5mg/L peptone, 300.0mg/L ammonium chloride, 30.0mg/L ferrous sulfate, 150.0mg/L anhydrous calcium chloride, 52.5mg/L dipotassium hydrogen phosphate, 22.5mg/L anhydrous potassium dihydrogen phosphate, 22.5mg/L magnesium sulfate, 1mL/L trace elements and a plurality of sodium bicarbonate;
wherein the microelement is H3BO4150mg/L，ZnSO4·7H2O 120mg/L，MnCl2·7H2O 120mg/L，CuSO4·5H2O 30mg/L，NiCl250mg/L，CoCl2·6H2O 210 mg/L，KI 30mg/L，Na2MoO465mg/L；
The bioflocculant fermentation liquor is prepared by fermentation of Klebsiella; the photosynthetic bacteria concentrated solution is zymocyte solution of rhodopseudomonas bacteria.
And 3, quickly starting and operating a bacteria-algae symbiotic system:
step 3.1, water inflow: in each reaction period, the inlet water is kept at 20-30 ℃, the inlet pump pours the sewage in the inlet tank into the descending pipe of the reactor main body through the inlet pipe, the inlet time of the inlet pump is set to be 3-6 minutes through the PLC, and the inlet pump is closed after the inlet water is finished.
Step 3.2, forming a coupling particle system: inoculating 1:10 (reactor sewage volume) concentrated algae liquid and 1:1000 (reactor sewage volume) of fungal spore suspension at the top of a descending pipe 6, simultaneously adding a certain amount of bioflocculant (1:100 (reactor sewage volume)) and photosynthetic bacteria concentrated liquid (1:100 (reactor sewage volume)), starting an air compressor, feeding compressed air into a reactor main body from an aeration head through an air inlet pipe, driving sewage in the reactor main body to move upwards in an ascending pipe under the driving of ascending air flow, lifting to a joint of the upper end of the ascending pipe and the upper end of the descending pipe, starting to enter the descending pipe, and then moving downwards to the bottom of the reactor; the fungal spores are aerated for 60 hours to gradually form mycelium pellets, the mycelium is intertwined in the pellet forming process, and the microalgae are tightly combined on the mycelium to form green mycelium pellets. The culture conditions were as follows: the temperature is 20-30 ℃, the air ventilation amount is 60-180L/h, the dissolved oxygen is 3.0mg/L, pH6-8, the illumination intensity is 5000-.
Step 3.3, stabilizing operation: and 3.2, allowing the bacteria and algae to jointly grow, culturing for a period of time to form bacteria and algae coupled particles, closing an air compressor to separate the bacteria and algae symbiont from sewage under the gravity condition, precipitating for 20 minutes, draining for 5 minutes, and allowing the reactor to enter an idle period of 120 minutes. In the stable operation period, the reactor adopts intermittent water feeding, and the operation conditions are as follows: the period is 12h, including water feeding in 5min, aeration at 570min (120L/h), precipitation for 20min, water draining at 5min and idle at 120 min.
After the treatment is finished, the cultured mycosphaerella globalpina is detected, the mycosphaerella globalpina has a compact structure, good precipitation performance and stability and good nitrogen and phosphorus removal capacity, the removal rate of total nitrogen is 93.9%, the removal rate of total phosphorus is 95.5%, the removal rate of COD is 90.0%, and the removal rate of NH4+ -N is 98.4% after the reactor runs stably.
Example 2
and 3, quickly starting and operating a bacteria-algae symbiotic system:
step 3.1, water inflow: same as example 1;
step 3.2, forming a coupling particle system: inoculating 1:10 (reactor sewage volume) concentrated algae liquid and crushed mycelia (200mg/L final inoculation concentration) at an inoculation port, simultaneously adding a certain amount of biological flocculant (1:50 (reactor sewage volume)) and photosynthetic bacteria concentrated solution (1:50 (reactor sewage volume)), starting an air compressor, feeding compressed air into a reactor main body from an aeration head through an air inlet pipe, driving sewage in the reactor main body to move upwards in an ascending pipe under the driving of ascending air flow, ascending to the joint of the upper end of the ascending pipe and the upper end of a descending pipe, starting to enter the descending pipe, and then moving downwards to the bottom of the reactor; the fungal spores are aerated for 36 hours to gradually form mycelium pellets, the mycelium is intertwined in the pellet forming process, and the microalgae are tightly combined on the mycelium to form green mycelium pellets. The culture conditions were as follows: the temperature is 20-30 ℃, the air ventilation amount is 60-180L/h, the dissolved oxygen is 3.0mg/L, pH6-8, the illumination intensity is 5000-.
Step 3.3, stabilizing operation: same as example 1;
after the treatment is finished, the cultured mycosphaerella globalpina is detected, the mycosphaerella globalpina has a compact structure, good precipitation performance and stability and good nitrogen and phosphorus removal capacity, the removal rate of total nitrogen is 96.2%, the removal rate of total phosphorus is 98.3%, the removal rate of COD is 91.2%, and the removal rate of NH4+ -N is 98.9% after the reactor runs stably.
Example 3
and 3, quickly starting and operating a bacteria-algae symbiotic system:
step 3.1, water inflow: same as example 1;
step 3.2, forming a coupling particle system: inoculating 1:10 (reactor sewage volume) concentrated algae liquid and mycelium pellets (400mg/L final inoculation concentration) at an inoculation port, simultaneously adding a certain amount of bioflocculant (1:150 (reactor sewage volume)) and photosynthetic bacteria concentrated liquid (1:150 (reactor sewage volume)), starting an air compressor, feeding compressed air into a reactor main body from an aeration head through an air inlet pipe, driving sewage in the reactor main body to move upwards in an ascending pipe under the drive of ascending air flow, lifting to a joint of the ascending pipe and the upper end of a descending pipe, starting to enter the descending pipe, and then moving downwards to the bottom of the reactor; the fungal spores are aerated for 12 hours to gradually form mycelium pellets, the mycelium is intertwined in the pellet forming process, and the microalgae are tightly combined on the mycelium to form green mycelium pellets. The culture conditions were as follows: the temperature is 20-30 ℃, the air ventilation amount is 60-180L/h, the dissolved oxygen is 3.0mg/L, pH6-8, the illumination intensity is 5000-.
Step 3.3, stabilizing operation: same as example 1;
after the treatment is finished, the cultured mycosphaerella globalpina is detected, the mycosphaerella globalpina has a compact structure, good precipitation performance and stability and good nitrogen and phosphorus removal capacity, the removal rate of total nitrogen is 92.9%, the removal rate of total phosphorus is 94.9%, the removal rate of COD is 90.5%, and the removal rate of NH4+ -N is 97.9% after the reactor runs stably.
While the foregoing description shows and describes several preferred embodiments of the invention, it is to be understood, as noted above, that the invention is not limited to the forms disclosed herein, but is not to be construed as excluding other embodiments and is capable of use in various other combinations, modifications, and environments and is capable of changes within the scope of the inventive concept as expressed herein, commensurate with the above teachings, or the skill or knowledge of the relevant art. And that modifications and variations may be effected by those skilled in the art without departing from the spirit and scope of the invention as defined by the appended claims.
Claims (5)
1. A waste water treatment method of an immobilized microalgae reactor taking mycelium pellets as a carrier is characterized by comprising the following steps:
step 1, manufacturing an immobilized microalgae reactor with mycelium pellets as carriers;
the immobilized microalgae taking the mycelium pellets as the carriers comprises a water inlet system, a reactor main body and a water outlet system, wherein the water inlet system comprises a water inlet tank (1), and the water inlet tank (1) is sequentially connected with a water inlet pump (2) and a water inlet pipe (11) through a pipeline; the reactor main body comprises an ascending pipe (5) and a descending pipe (6); the other end of the water inlet pipe (11) is communicated with the top end of the downcomer (6), the ascending pipe (5) is positioned in the pipe cavity of the downcomer (6), and the ascending pipe (5) and the downcomer (6) are sleeved coaxially from inside to outside; an aeration head (8) is arranged at the bottom of the downcomer (6), and the aeration head (8) is communicated with an air compressor (7) through an air inlet pipe (13); the water outlet system comprises a water outlet tank (4) and a water outlet pipe (12), wherein the water outlet pipe (12) is arranged on the side wall of the downcomer (6); the water outlet pipe (12) is communicated with the down pipe (6), and the water outlet pipe (12) discharges water to the water outlet tank (4);
the water outlet system also comprises an electromagnetic water outlet valve (3), the electromagnetic water outlet valve (3) is arranged on the water outlet pipe (12) and is communicated with the water outlet pipe (12), the side wall of the downcomer (6) is also provided with uniformly distributed sampling ports (14), and the sampling ports (14) are arranged opposite to the water outlet pipe (12);
the water outlet pipe (12) is arranged at the position of 1/2-2/3 of the bottom of the ascending pipe (5), and the ratio of the height of the ascending pipe (5) to the inner diameter of the ascending pipe (5) is 1: 4-6; the ratio of the height of the downcomer (6) to the inner diameter of the downcomer (6) is 1: 4-7;
the reactor also comprises a PLC (programmable logic controller) (9), wherein the PLC (9) is respectively connected with the water inlet pump (2), the electromagnetic water outlet valve (3), the air compressor (7), the external light source (20), the temperature controller (15), the dissolved oxygen sensor (16) and the pH sensor (17) through leads; the external light source (20) is arranged on the external light source bracket (19), and the external light source bracket (19) is arranged on the outer side wall of the descending tube (6);
the top of the downcomer (6) is provided with a reactor cover (21), and the reactor cover (21) is provided with fixing holes for fixing a temperature controller (15), a dissolved oxygen sensor (16), a pH sensor (17) and a water inlet pipe (11);
step 2, preparing concentrated algae solution, mycelium pellet suspension, bioflocculant fermentation liquor and photosynthetic bacteria concentrated solution;
step 3, rapidly starting and operating the bacteria-algae symbiotic system;
the reactor increases the contact mixing effect of the sewage, algae and fungi in the water inlet stage of the reactor, and a coupling system is formed in a way of adding mycelium pellet suspension and microalgae together.
2. The wastewater treatment method according to claim 1, wherein the mycelium pellet suspension in the step 2 comprises a fungal spore suspension or a broken mycelium suspension;
the fungal spore suspension is prepared by the following method: transferring the fungal spores on the inclined plane into sterile water containing glass beads, suspending the spores on the inclined plane in the water, placing the suspension containing the glass beads in a shaking table at 180rpm, and shaking for 2h to fully disperse the spores and ensure that each milliliter contains 10 spores8-109cfu; the method is used for culturing the bacteria-algae coupling system, and the inoculation amount of spore suspension is determined to be 1mL/1000mL of bacteria-algae coupling culture medium;
fungus Aspergillus niger is selected as fungal spore;
the broken mycelium suspension was prepared by the following method: transferring the cultured mature mycelium pellets into sterile water filled with glass beads, placing the mycelium pellets in a shaking table at 180rpm, shaking for 30min, and crushing for 30s by using a stirrer; inoculating the obtained hypha fragments into a fungus-algae coupling culture medium to complete the process of mycelial ball culture;
the concentrated algae solution is prepared by the following steps: inoculating microalgae into high-density culture microalgae shake flask, controlling temperature within 28-32 deg.C, and optimum illumination at 30 deg.C, wherein the culture medium is bacteria-algae coupled culture medium with illumination intensity of 5000Lux, pH value of 7.0, and culture time of96-120 hours; culturing under the above suitable conditions to reach cell density of 109-1010The microalgae is finally transferred into a reactor, and the inoculation amount is 100mL/1000mL of the bacteria-algae coupling culture medium;
the bacteria-algae coupling culture medium is simulated food wastewater, and the formula of the bacteria-algae coupling culture medium is as follows: 1500.0mg/L glucose, 75.0mg/L beef extract, 112.5mg/L peptone, 300.0mg/L ammonium chloride, 30.0mg/L ferrous sulfate, 150.0mg/L anhydrous calcium chloride, 52.5mg/L dipotassium hydrogen phosphate, 22.5mg/L anhydrous potassium dihydrogen phosphate, 22.5mg/L magnesium sulfate, 1mL/L trace elements and a plurality of sodium bicarbonate;
wherein the microelement is H3BO4150mg/L，ZnSO4·7H2O120mg/L，MnCl2·7H2O120mg/L，CuSO4·5H2O30mg/L，NiCl250mg/L，CoCl2·6H2O210mg/L，KI30mg/L，Na2MoO465mg/L；
The bioflocculant fermentation liquor is prepared by fermentation of Klebsiella; the photosynthetic bacteria concentrated solution is zymocyte solution of rhodopseudomonas bacteria.
3. The wastewater treatment method according to claim 2, wherein the fast start-up and operation of the phycobiont system in step 3 specifically comprises:
step 3.1, water inflow: in each reaction period, the inlet water is kept at 20-30 ℃, the inlet water pump (2) leads the simulated wastewater in the inlet water tank (1) into a downcomer (6) of the reactor main body through an inlet pipe (11), the inlet water time of the inlet water pump (2) is set to be 3-6 minutes through a PLC (programmable logic controller) controller (9), and the inlet water pump (2) is closed after the inlet water is finished;
step 3.2, forming a coupling particle system: inoculating a certain amount of concentrated algae liquid and one of fungal spore suspension, mycelial ball suspension or broken mycelium suspension at an opening at the upper end of a reactor main body, simultaneously adding a certain amount of bioflocculant fermentation liquid and photosynthetic bacteria concentrated liquid, starting an air compressor (7), supplying compressed air into the reactor main body from an aeration head (8) through an air inlet pipe (13), enabling wastewater in the reactor main body to move upwards in an ascending pipe (5) under the driving of ascending air flow, enabling the wastewater to rise to the joint of the upper end of the ascending pipe (5) and the upper end of a descending pipe (6) to enter the descending pipe (6), and then moving downwards to the bottom of the reactor; aerating for 12-60 hours to gradually form mycelium pellets by fungal spores or broken mycelium, wherein the mycelium is mutually wound in the pellet forming process, and the microalgae are tightly combined on the mycelium to form green mycelium pellets;
step 3.3, stabilizing operation: step 3.2, allowing bacteria and algae to jointly grow, culturing for a period of time to form bacteria and algae coupling particles, closing an air compressor (7) to separate the bacteria and algae symbiont from sewage under the gravity condition, precipitating for 1 minute, draining for 5 minutes, and allowing the reactor to enter an idle period of 120 minutes; and starting to enter a stable operation period, and adopting intermittent water feeding for the reactor.
4. The wastewater treatment method according to claim 3, wherein the volume ratio of the addition amount of the fungal spore suspension in step 3.2 to the reactor wastewater is 1: 1000; the final inoculation concentration of the mycelium pellet suspension is 400mg/L, and the final inoculation concentration of the broken mycelium suspension is 200 mg/L; the volume ratio of the addition amount of the concentrated algae liquid to the sewage of the reactor is 1: 10; the volume ratio of the bioflocculant fermentation liquor to the reactor sewage is 1:50-1: 150; the volume ratio of the photosynthetic bacteria concentrated solution to the reactor sewage is 1:50-1: 150; the culture conditions for the coupled particle system were: the temperature is 20-30 ℃, the air ventilation amount is 60-180L/h, the dissolved oxygen is 3.0mg/L, pH6-8, the illumination intensity is 5000-.
5. A method for treating waste water according to claim 3, characterized in that the operating conditions during steady operation in step 3.2 are as follows: the period is 12h, including water feeding in 5min, aeration for 589min, aeration amount of 120L/h, precipitation for 1min, water draining for 5min and standing for 120 min.
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