CN109223986A - A kind of compound dendrobium officinale oral solution and preparation method thereof - Google Patents
A kind of compound dendrobium officinale oral solution and preparation method thereof Download PDFInfo
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Abstract
A kind of compound dendrobium officinale oral solution of the invention and preparation method thereof, comprising steps of the dendrobium candidum of corresponding parts by weight, Radix Angelicae Sinensis, Radix Paeoniae Alba, fingered citron, rose and chrysanthemum are soaked in water 0.5~3h by S100, the water that 8~12 times of amounts are added decocts 2~3 times, 1~2h every time, the dregs of a decoction are filtered off, extracting solution is obtained；S200 is concentrated into 2.5~3.5 times of medicinal material amount at 50~70 DEG C, obtains concentrate；Corrigent and preservative, stirring and dissolving, constant volume, encapsulating, sterilizing is added in 4~25 DEG C of standing 4h or more, filtering or centrifugation in S300.To help to improve inoxidizability and improve sleep, improve cell viability by innovation formula, preparation and formulation process method.
Description
Technical field
The present invention relates to pharmaceutical technology fields, specifically, being a kind of with anti-oxidant and improvement sleep effect Chinese medicine
Compound dendrobium officinale oral solution and preparation method thereof.
Background technique
Dendrobium candidum (Dendrobium officinale) is orchid family Dendrobium herbaceos perennial, main medicinal
Position is fresh or dry stem, is distributed mainly on the ground such as Anhui, Zhejiang, Guangxi, Hunan, Yunnan, Guizhou, is traditional rare Chinese medicine
Material.Dendrobium candidum has reinforcing stomach reg fluid, the effect of nourishing Yin and clearing heat, is used for impairment of yin body fluid deficiency, dry polydipsia, deficiency of stomach-Yin, and deficiency of food is dry
It vomits, abnormal heat is not moved back after being ill, and fire excess from yin deficiency, hectic fever due to yin labor heat, mesh is secretly unknown, the diseases such as muscles and bones impotence.Shennong's Herbal is classified as
It is described as first of " Chinese nine big mesonas " by top grade, Taoism regimen classics " Taoist Scriptures ".
Exploitation around dendrobium candidum and its Related product is always the popular domain of health food development.Currently, from group
From the point of view of in side, dendrobium candidum polygamy is with boosting qi and nourishing yin, the American Ginseng of clearing heat and promoting fluid, the Radix Ophiopogonis of enriching yin, rhizoma polygonati, radix polygonati officinalis, and mends
Gas tranquilizing the mind ganoderma lucidum etc., with three taste medicines, simple side below is in the majority.Due to being mostly cool property medicine, many user's reflections are excessively cold
It is cool, cause weakness of the spleen and the stomach.In addition, being directed to the prescription of women, also there is dendrobium candidum with simple side's listing such as west safflower, but can not change
Kind sleep.
Summary of the invention
The main purpose of the present invention is to provide a kind of compound dendrobium officinale oral solutions and preparation method thereof, pass through innovation
Formula, preparation and formulation process method help to improve inoxidizability and improve sleep, improve cell viability.
To achieve the above objectives, the technical solution adopted by the present invention are as follows: a kind of compound dendrobium officinale oral solution includes following
The raw material of parts by weight meter: 1.5-2.0 parts of dendrobium candidums, 0.5-2.0 parts of Radix Angelicae Sinensis, 1.0-1.5 portions of Radix Paeoniae Albas, 1.5-2.0 portions of fingered citrons,
1.0-1.5 portions of roses, 1.0-1.5 parts of chrysanthemums.
A kind of preparation method of compound dendrobium officinale oral solution comprising steps of
The dendrobium candidum of corresponding parts by weight, Radix Angelicae Sinensis, Radix Paeoniae Alba, fingered citron, rose and chrysanthemum be soaked in water 0.5 by S100~
3h, the water that 8~12 times of amounts are added decoct 2~3 times, and 1~2h, filters off the dregs of a decoction, obtain extracting solution every time；
S200 is concentrated into 2.5~3.5 times of medicinal material amount at 50~70 DEG C, obtains concentrate；
S300 is filtered or is centrifuged in 4~25 DEG C of standing 4h or more, addition corrigent and preservative, stirring and dissolving, constant volume,
Encapsulating, sterilizing.
Flocculant, the flocculant choosing is added after concentrate is made in step S200 in an embodiment according to the present invention
From one of chitosan, gelatin, sodium alginate, xanthan gum, pectin or a variety of.
Preferably, the step S100 specifically includes step and medicinal material is soaked in water 1h, and the water measured with 12 times decocts 2 times,
First time 1.5h, second of 1.0h filter off the dregs of a decoction, obtain extracting solution, wherein heating extraction temperature is 70~100 DEG C, with abundant
Subject to reflux.
Preferably, the step S200 specifically includes step is concentrated into medicinal material amount at 60 DEG C with weight by volume basis 3
Times, relative density is 1.040~1.050, obtains concentrate.
Preferably, the gelatin solution that gelatin flocculant is 8%.
Preferably, the volume ratio of concentrate and gelatin flocculant is 3:1, is sufficiently stirred.
Preferably, the step S300 specifically includes step and stands, plate-frame filtering, 2% mannitol of addition, 2% xylitol,
0.04% potassium sorbate, stirring and dissolving, constant volume, encapsulating, sterilizing.
Preferably, the dwell temperature in the step S300 is 4 DEG C.
Preferably, the time of repose in the step S300 is 4h.
Compared with prior art, the invention has the benefit that being set out with nourishing yin and supplementing blood, blood-nourishing, by dendrobium candidum with work as
Return, Radix Paeoniae Alba plus-minus combination, and then the fingered citron of qi-regulating soothing liver-qi stagnation of arranging in pairs or groups, the chrysanthemum of rose and heat dissipation of clearing liver and improving vision, forms
A kind of nourishing yin and supplementing blood, the innovation prescription of qi-regulating soothing liver-qi stagnation.Wherein this side is using dendrobium candidum as monarch drug in a prescription, with enriching blood in Siwu Tang
Key medicine Radix Angelicae Sinensis and white peony root is used as ministerial medicine are assistant with the fingered citron of qi-regulating soothing liver-qi stagnation, rose, and the chrysanthemum with heat dissipation of clearing liver and improving vision is
Make, improve inoxidizability and improves sleep.
Detailed description of the invention
Shown in FIG. 1 is Thick many candies canonical plotting in embodiment according to the present invention.
It is shown in Fig. 2 be in embodiment according to the present invention oral solution to the influence diagram of cell viability.
Specific embodiment
It is described below for disclosing the present invention so that those skilled in the art can be realized the present invention.It is excellent in being described below
Embodiment is selected to be only used as illustrating, it may occur to persons skilled in the art that other obvious modifications.
Embodiment 1
A kind of preparation method of compound dendrobium officinale oral solution comprising steps of
60g dendrobium candidum, 30g Radix Angelicae Sinensis, 45g Radix Paeoniae Alba, 60g fingered citron, 30g rose, 30g chrysanthemum are soaked in water 2h, used
The water of 10 times of amounts decocts 2 times, first time 1.5h, second of 1.0h, filters off the dregs of a decoction, obtains extracting solution；It is concentrated at 55 DEG C
750mL or so obtains concentrate.8% gelatin flocculant 250mL is added by 3:1 (volume ratio) in concentrate, is sufficiently stirred, at 4 DEG C
It stands for 24 hours, plate-frame filtering, 2% mannitol of addition, 2% xylitol, 0.04% potassium sorbate, stirring and dissolving is settled to
1000mL, 20mL/ bottles of encapsulating, sterilizing to get.
Preparation process correlative study is as follows:
Embodiment 2 is to embodiment 6
The choice experiment of flocculant
The same embodiment of oral preparation preparation method of embodiment 2~6, the difference is that the selection of flocculant, embodiment 2
In flocculant be chitosan solution: weigh a certain amount of chitosan solid, glacial acetic acid be added, be configured to 1% chitosan it is molten
Liquid is swollen spare for 24 hours.
Flocculant in embodiment 3 is gelatin solution: quantitative gelatin is added deionized water, is configured to 2% solution, molten
It is swollen spare for 24 hours.
Flocculant in embodiment 4 is sodium alginate soln: being configured to 1% solution with deionized water, is swollen standby for 24 hours
With.
Flocculant in embodiment 5 is xanthan gum solution: 1% solution is configured to, at paste.
Flocculant in embodiment 6 is pectin solution: it is configured to 1% solution, it is muddy.
Two parts of concentrate 30mL in Example 1 respectively, in 50mL EP pipe；The configured chitosan of 10mL is added
Flocculant is quickly stirred with glass rod, and 1 part is placed in 4 DEG C of refrigerators, and 1 part stands at room temperature；After standing 4h, 5000r/min stirring, from
Heart 5min.
Meanwhile operating every kind of flocculant solution in embodiment 3~6 according to above-mentioned steps, supernatant is compared in observation
Clarity.
Conclusion: flocculant selects gelatin, stands 4h at room temperature, processed oral solution clarity is best.
Embodiment 7 is to embodiment 11
Influence of 1.2 flocculants to effective component
(1) sulfuric acid-phynol spectrophotometry surveys Thick many candies content, Thick many candies standard curve as shown in Figure 1.
(2) in the oral preparation of different disposal method Thick many candies concentration, embodiment 7~implementation 9 oral preparation preparation
Method is with embodiment 1, the difference is that the processing method stood is different, the results are shown in Table 1.
The concentration of Thick many candies in the oral solution of 1 embodiment of table, 7~9 different disposal method
(3) the preparation method is the same as that of Example 1 for the oral preparation of embodiment 10~11, the difference is that, implement quiet in 10
The method of setting is that gelatin flocculant is added in concentrate to stand 4h, and implementing 11 is that gelatin flocculant is not added only to stand 4h, is shown in Table 2 institutes
Show.
The concentration of Thick many candies in the oral solution of flocculant is added in table 2
Conclusion: gelatin, which is added, influences less the concentration of polysaccharide.
Embodiment 12 is to embodiment 16
Concentration, the dosage of 1.3 flocculant gelatin are investigated
Gelatin concentration in embodiment 12~16 is different, and gelatin solution configures successively are as follows: configuration 1%, 3%, 5%, 8%,
16% solution is swollen spare for 24 hours.Again by the 30mL concentrate in embodiment 1 in 50mL EP pipe, it is separately added into different dense
The gelatin solution 10mL of degree is quickly stirred with glass rod, is stood at room temperature；After standing 4h, 5000r/min is centrifuged 5min；Observation ratio
Occur the muddy time compared with supernatant, is shown in Table 3.
Influence of the concentration of gelatin solution to clarity is added in table 3
Conclusion: preferably, the flocculant is to select 8% gelatin solution.
Embodiment 17 is to embodiment 22
The dosage of gelatin is different in embodiment 17~22, by the 30mL concentrate in embodiment 1 in 50mL EP pipe, point
Not Jia Ru 2,8% gelatin solution of 4,6,8,10,15mL, quickly stirred with glass rod, stood at room temperature；After standing 4h, 5000r/
Min is centrifuged 5min；Observation compares supernatant and the muddy time occurs, the results are shown in Table shown in 4.
Influence of the amount of gelatin solution to clarity is added in table 4
Conclusion: 8% gelatin solution of result selection 10mL.
The investigation of 1.4 dwell temperatures
30mL concentrate is set in 50mL EP pipe, 8% gelatin solution of 10mL is added, is quickly stirred with glass rod, respectively at
4 DEG C, stand at room temperature, parallel 3 parts；After continuously standing several hours, 5000r/min, 5min centrifugation；Observation is compared supernatant and is gone out
It the existing muddy time, the results are shown in Table shown in 5.
Influence of 5 dwell temperature of table to clarity
Conclusion: it is shorter to stand the clear and bright time at room temperature, comprehensively considers and stands at 4 DEG C of selection.
1.5 time of repose
30mL concentrate is set in 50mL EP pipe, 8% gelatin solution of 10mL is added, is quickly stirred with glass rod, respectively at
4 DEG C of standings 1,4,8h；Some time after standing, 5000r/min, 5min centrifugation；Observation compares supernatant and the muddy time occurs,
It the results are shown in Table shown in 6.
Influence of 6 time of repose of table to clarity
Conclusion: 4h or more is stood.
The screening of 1.6 corrigents
The selection of corrigent and the results are shown in Table 7.
7 corrigent of table screens table
| Lactose | 3% | 2% | |||
| Xylitol | 4% | 2% | 2% | ||
| Mannitol | 2% | 2% | |||
| Steviol glycoside | 0.02% | 0.01% | |||
| Citric acid | 2% | 1% | |||
| Malic acid | 1% | ||||
| Mouthfeel | It is sweet | First sweet tea, it is rear sour and astringent | Too sweet tea | It is sweetless, sour | Sweet taste is moderate |
Conclusion: selection addition 2% xylitol, 2% mannitol.
Embodiment 23
A kind of preparation method of antioxidant oral preparation comprising steps of
60g dendrobium candidum, 60g Radix Angelicae Sinensis, 45g Radix Paeoniae Alba, 60g fingered citron, 45g rose, 45g chrysanthemum are soaked in water 1h, used
The water of 12 times of amounts decocts 2 times, each 1.5h, filters off the dregs of a decoction, obtains extracting solution；It is concentrated into 900mL or so at 60 DEG C, is obtained dense
Contracting liquid.8% gelatin flocculant 300mL is added by 3:1 (volume ratio) in concentrate, is sufficiently stirred, in 4 DEG C of standing 4h, plate-frame filtering,
It is added 2% mannitol, 2% xylitol, 0.04% potassium sorbate, stirring and dissolving is settled to 1200mL, 20mL/ bottles of encapsulating, goes out
Compound dendrobium officinale oral solution is made in bacterium.
The outer protective effect to peroxide injury cell of compound dendrobium officinale oral solution made from embodiment 23
3.1 materials and methods: Bioengineering Research Institute is built up in superoxide dismutase (SOD) testing cassete, Nanjing, lot number:
20180508；Bioengineering Research Institute is built up in micro malonaldehyde (MDA) testing cassete, Nanjing, lot number: 20180516；Lactic dehydrogenase
Bioengineering Research Institute is built up in enzyme (LDH) assay kit, Nanjing, lot number 20180518:PBS: Wuhan doctor's moral bioengineering
Co., Ltd, lot number: 11D16B30；Pancreatin: gibco, lot number: 1982763；MTT powder, BIOSHARP, lot number: 0793；DMEM
Culture medium: Vicente Bioisystech Co., Ltd, lot number: 319005109；Fetal calf serum, the South America Gemini, A80E00G；Diformazan
Base sulfoxide (DMSO), Biofroxx, lot number: EZ2811E252.
Test cell: Human umbilical vein endothelial cells HUVECs
3.2 cell culture and preliminary experiment:
It takes the Human umbilical vein endothelial cells frozen to recover, is trained with containing 1% dual anti-, 10% fetal calf serum DMEM culture medium
It supports, changes a not good liquor within every two days, be used for subsequent experimental.
Grope hydrogen peroxide and most preferably damage concentration:
Different concentration of hydrogen peroxide damaging cells are set, and cell density is adjusted to 5 × 104/ mL, each concentration are arranged 5
The MTT solution of final concentration of 5g/L is added after Hydroperoxide injury 4h is added with 96 orifice plate culture cells in multiple holes in each hole
20 μ L continue to terminate culture after cultivating 4h, and 150 μ L dimethyl sulfoxides (DMSO), 37 DEG C of incubations are added in every hole after sucking supernatant
10min vibrates 10min.After endoparticle is completely dissolved after hole, each hole absorbance A is measured at 490nm.It is living to analyze group of cells
Property, determine that optimal hydrogen peroxide damages concentration.When concentration of hydrogen peroxide is 400 μm of ol/L, cell growth is significantly suppressed,
And inhibition level is not excessive, therefore subsequent experimental chooses the concentration as the damage concentration to Human umbilical vein endothelial cells.
Cell survival rate analysis:
The compound dendrobium officinale oral solution of various concentration pre-processes Human umbilical vein endothelial cells 12h, then with 400 μm of ol/L
Cell viability is detected using mtt assay after hydrogen peroxide treatment 4h.
3.3 cellular oxidations and anti-oxidant detection
Cell kind is grouped into blank group in 6 orifice plates, and model group is administered totally 5 groups of high, medium and low dosage group, every group setting 3
Multiple holes.Cultivate after cell kind plate makes its adherent for 24 hours, and drug effect 12h is then added, H is added after 12h2O2Damaging cells 4h is received
Collect cell conditioned medium to detect for LDH.Trypsin digestion cell collects cell in centrifuge tube, and PBS cleaning is primary, and 400 μ are added in centrifugation
LPBS requires to detect indices after ultrasonic disruption cell according to kit specification.BCA method measurement albumen contains
Amount calculates separately each group SOD activity and LDH, MDA content.
3.4 statistical method
Data are indicated with means standard deviation, for statistical analysis using EXCEL software.Two comparison among groups are examined using t,
More comparison among groups use one-way analysis of variance.
3.5 result
(1) cell viability testing result:
Compared to the blank group, H2O2Cell-proliferation activity after damage 4h is significantly suppressed.And with 1/100,1/200,1/
After 400 diluted compound dendrobium officinale oral solution pretreatment Human umbilical vein endothelial cells 12h, mtt assay detects high, middle dose group
Cell-proliferation activity is significantly protected.Illustrate compound dendrobium officinale oral solution to H2O2The HUVECs of damage has significant protect
Shield effect, as shown in Figure 2.
(2) influence of the compound dendrobium officinale oral solution to cell LDH, SOD, MDA level:
Compared to the blank group, H2O2LDH, MDA content of the HUVECs of induced damage is remarkably decreased, and SOD vigor rises.With
Model group is compared, and cell SOD vigor can be improved in compound dendrobium officinale oral solution, it is horizontal to reduce cell MDA, but cell is trained
It supports supernatant LDH level and has no that reduction acts on, as shown in table 8.
8 compound dendrobium officinale oral solution of table is to H2O2Damage the MDA of HUVECs, LDH content and the active influence of SOD
The experiment of peroxide injury endothelial cell is shown: compared to the blank group, H2O2HUVECs proliferation activity after damaging 4H
Significantly it is suppressed；Cell supernatant LDH, MDA content significantly increases, and SOD vigor reduces.And with 1/100,1/200,1/400
After diluted compound dendrobium officinale oral solution pretreatment Human umbilical vein endothelial cells 12h, mtt assay detects that high, middle dose group is thin
Born of the same parents' proliferation activity increases (P < 0.05,0.01)；Cell supernatant MDA content reduces, and SOD vigor increases (P < 0.05,0.01).With
Upper result illustrates the anti-oxidant compound dendrobium officinale oral preparation to H2O2The HUVECs of damage has significant protective effect.
Internal Oxidation Resistance Test
4.1 materials and methods
Bioengineering Research Institute is built up in micro reduced glutathione testing cassete, Nanjing, lot number: 20180508；Super oxygen
Bioengineering Research Institute is built up in object mutase (SOD) testing cassete, Nanjing, lot number: 20180508；Malonaldehyde (MDA) testing cassete, south
Bioengineering Research Institute is built up in capital, lot number: 20180507；BCA determination of protein concentration kit, green skies biotechnology research
Institute, lot number: 081517180208；Ethyl alcohol (dehydrated alcohol), Sinopharm Chemical Reagent Co., Ltd., lot number: 20171204；Second
Sour (glacial acetic acid), Sinopharm Chemical Reagent Co., Ltd., lot number: 20160309.
4.2 experimental animals:
It SPF grades ICR August age male mice 70, is provided by Jiangning county Qinglongshan animal reproduction field, animal is permitted
Can the number of card: SCXK (Soviet Union) 2017-0001 be raised in Nanjing University of Traditional Chinese Medicine's Experimental Animal Center, credit number: SYXK (Soviet Union)
2014-0001.The raising of animal complete feed, free water are 22 DEG C -25 DEG C of temperature, relative humidity 50%-60%, daily artificial
Illumination 12 hours.Before experiment, mouse adaptive feeding 3 days.
4.3 experimental methods:
The concentration of compound dendrobium officinale oral solution:
Original oral liquid rotary evaporation is prepared into concentrate, administered volume is by weight of the oral solution volume of concentration front and back.
Animal packet:
70 aged mouse adaptable feds, 3 days posterior orbits take blood, are horizontally divided into aged controls group, drug by MDA in blood
Totally 4 groups of high, medium and low dosage group.
Medication:
The high, medium and low dosage group of experimental animal is according to compound dendrobium officinale oral solution stoste 0.28mL/10g, 0.14mL/
The administered volume stomach-filling of 10g, 0.07mL/10g 30 days, once a day, aged controls group gives physiological saline 0.1mL/10g.End
Secondary administration posterior orbit takes blood, and mouse takes off neck and puts to death, and takes liver organization.
Indexs measure:
Mouse blood is stored at room temperature 2h, and 3000r/min is centrifuged 10min, pipettes supernatant, -80 DEG C of preservations.Weigh 50mg liver group
It knits, adds 450 μ L physiological saline in preparing 10% homogenate on ice.It is dense by kit specification requirement measurement tissue homogenate proteins
Degree, detects serum and liver homogenate GSH, SOD, MDA are horizontal.
Statistical method:
Data are indicated with means standard deviation, for statistical analysis using EXCEL software.Two comparison among groups are examined using t,
More comparison among groups use one-way analysis of variance.
4.4 experimental result
(1) influence of the compound dendrobium officinale oral solution to aged mouse weight and general state
Have during experiment some animals because caused by hitting mutually severe trauma it is lethal, without merely because murderous feelings are administered
Condition.Without significant difference, liver organ coefficient, which also has no, to be significantly changed for mouse weight variation during experiment.As shown in table 9.
Influence of the oral preparation to aged mouse liver coefficient in 9 embodiment 23 of table
(2) influence of the compound dendrobium officinale oral solution to aged mouse serum oxidation resistance
Compared with aged controls group, compound dendrobium officinale oral solution does not increase effect to GSH level in aged mouse serum
Fruit；Middle dosage can significantly increase aged mouse SOD in serum level, and high and low dose, which has, increases becoming for aged mouse SOD in serum
Gesture；In, low dosage can significantly reduce that aged mouse serum MDA is horizontal, and high dose has the tendency that reducing MDA.As shown in table 10.
Influence of the 10 compound dendrobium officinale oral solution of table to GSH, SOD and MDA level in aged mouse serum
(3) influence of the compound dendrobium officinale oral solution to aged mouse hepatic tissue oxidation resistance
Compared with aged controls group, compound dendrobium officinale oral solution is high, middle dosage can significantly increase aged mouse liver group
GSH level is knitted, low dosage has the tendency that increasing hepatic tissue GSH level；It is horizontal that high dose can significantly increase hepatic tissue SOD, low,
Middle dosage has the tendency that increased SOD；Low dosage can significantly reduce hepatic tissue MDA level, and high, middle dosage has becoming for reduction MDA
Gesture.As shown in table 11.
Influence of the 11 compound dendrobium officinale oral solution of table to GSH, SOD and MDA level in aged mouse hepatic tissue
4.5 conclusions:
Three dosage groups improve antioxidant enzyme (GSH and SOD) activity to some extent, reduce serum and liver
The content of oxidative injury markers MDA, to show the antioxidant activity of compound dendrobium officinale oral solution.
Embodiment 5 improves sleep experiments
5.1 materials and methods
Yellow Jackets: Merck KGaA company, lot number: WS20141110
5.2 experimental animals:
It SPF grades ICR20-23g male mice 120, is provided by Jiangning county Qinglongshan animal reproduction field, animal is permitted
Can the number of card: SCXK (Soviet Union) 2017-0001 be raised in Nanjing University of Traditional Chinese Medicine's Experimental Animal Center, credit number: SYXK (Soviet Union)
2014-0001.The raising of animal complete feed, free water are 22 DEG C -25 DEG C of temperature, relative humidity 50%-60%, daily artificial
Illumination 12 hours.Before experiment, mouse adaptive feeding 3 days.
5.3 experimental method
The concentration of compound dendrobium officinale oral solution:
Original oral liquid rotary evaporation is prepared into concentrate, administered volume is by weight of the oral solution volume of concentration front and back.
Animal packet:
120 are divided after aged mouse adaptable fed 3 days at random in three son experiments, and every height experiment is divided into blank pair
According to group, the high, medium and low dosage group of drug, totally 12 groups, every group 10.
Medication:
The high, medium and low dosage group of experimental animal is according to compound dendrobium officinale oral solution stoste 0.28mL/10g, 0.14mL/
The administered volume stomach-filling of 10g, 0.07mL/10g 30 days, once a day, blank control group gives physiological saline 0.1mL/10g.End
Mice sleep test is carried out after secondary administration 1h.
Direct sleep experiments:
After last dose 1h, mice sleep situation is observed.Sleep is crouched using righting reflex loss as index when mouse is in back
When position, body position can be righted immediately, if it exceeds 30s mouse cannot right, then it is assumed that righting reflex loss, mouse, which enters, to sleep
It sleeps.It is the sleeping time of mouse from righting reflex loss to its time restored.It observes respectively and calculates each administration group and blank
Sleep number of animals, sleep incidence and the sleeping time of control group.As the sleep number of elements of administration group mouse and sleeping time increase
And there is significant difference compared with the control group, that is, determine this result for the positive.
Extend the experiment of yellow Jackets sleeping time:
Prerun is first carried out before formal test, first carries out preliminary experiment before doing formal experiment, determining makes animal 100% fall asleep, but
Do not make the yellow Jackets dosage 40mg/kg that sleeping time is too long again, is formally tested with this dosage.After animal last dose 1h,
Yellow Jackets, injection volume 0.1mL/10g is injected intraperitoneally to groups of animals.Using righting reflex loss as index, compound is observed
Can dendrobium candidum oral solution extend yellow Jackets sleeping time.Sleeping time increase and and control group such as administration group mouse
Compared to significant difference, that is, determining this result for the positive.
Dosage hypnosis is tested under yellow Jackets domain:
Preliminary experiment is first carried out before formal experiment, determines yellow Jackets maximum subthreshold hypnotic dosage 30mg/kg, i.e., in this agent
The mouse righting reflex for measuring lower 80-90% does not disappear.After animal last dose 1h, yellow Jackets are injected intraperitoneally in groups of animals,
Injection volume is 0.1mL/10g.Sleep number of animals (righting reflex loss up to 1 minute or more person) in record 30 minutes.Such as administration group
The sleep rate of mouse increases and has significant difference compared with the control group, that is, determines this result for the positive.
Statistical method:
Data are handled using EXCEL statistical software.As a result it is indicated with means standard deviation, Dan Yin is used between multiple groups
Plain variance analysis；Sleep number of animals use Chi-square Test, and P < 0.05 indicates that there were significant differences.
5.4 result
(1) influence of compound dendrobium officinale Oral Liquid On Mice weight
Each group mouse weight steady growth during administration, and between each group mouse weight without significant difference.
(2) influence that compound dendrobium officinale Oral Liquid On Mice is directly slept
Experimental mice has piecemeal activity reduction upon administration, but does not occur sleep phenomenon.Illustrate compound dendrobium officinale
Oral Liquid On Mice is acted on without directly sleep, and this item experimental result is feminine gender.As shown in table 12.
The directly sleep of 12 compound dendrobium officinale oral solution of table acts on
(3) influence of the compound dendrobium officinale oral solution to the effect of mouse yellow Jackets sleeping time is extended
It is small with increasing for compound dendrobium officinale oral solution Dosages under yellow Jackets (40mg/kg) syngignoscism
The sleeping time of mouse gradually extends, in certain dose-dependence；Compared with blank control group, compound dendrobium officinale oral solution
The sleeping time of middle and high dosage group mouse extremely significant extension (P < 0.01), the sleeping time of middle dose group mouse has extension to become
Gesture illustrates that compound dendrobium officinale oral solution can extend the mouse sleep time of yellow Jackets induction, and experimental result is the positive.Such as
Shown in table 13.
Influence (40mg/kg) of the 13 compound dendrobium officinale oral solution of table to yellow Jackets sleeping time is extended
(4) influence of the compound dendrobium officinale oral solution to yellow Jackets sub-threshold dose syngignoscism
Under yellow Jackets (30mg/kg) effect, compared with blank control group, compound dendrobium officinale oral solution high dose
Group mouse sleep number of elements dramatically increases (P < 0.05), but low, middle dose group to yellow Jackets sub-threshold dose syngignoscism without aobvious
Writing influences, and illustrates that high dose compound dendrobium officinale oral solution cooperates with syngignoscism, experimental result with sub-threshold dose barbital sodium
It is positive.As shown in table 14.
Influence (30mg/kg) of the 14 compound dendrobium officinale oral solution of table to yellow Jackets sub-threshold dose syngignoscism
Note: * indicates that p < 0.05, * * indicate p < 0.01 in report
5.5 conclusion
Mouse directly sleeps, extends mouse yellow Jackets sleeping time and to yellow Jackets sub-threshold dose syngignoscism
Three experiments show: compound dendrobium officinale Oral Liquid On Mice is acted on without directly sleep, the extremely significant extension of high dose group mouse
Yellow Jackets sleeping time (P < 0.01), with increasing for compound dendrobium officinale oral solution Dosages, under yellow Jackets threshold
The sleeping time of dosage mouse gradually extends, and in certain dose-dependence, illustrates under compound dendrobium officinale oral solution and threshold
Dosage barbital sodium has collaboration syngignoscism.
The basic principles, main features and advantages of the present invention have been shown and described above.The technology of the industry
Personnel are it should be appreciated that the present invention is not limited to the above embodiments, and what is described in the above embodiment and the description is only the present invention
Principle, various changes and improvements may be made to the invention without departing from the spirit and scope of the present invention, these variation and
Improvement is both fallen in the range of claimed invention.The present invention claims protection scope by appended claims and its
Equivalent defines.
Claims (10)
1. a kind of compound dendrobium officinale oral solution, which is characterized in that the raw material including following parts by weight meter: 1.5~2.0 parts of iron sheets
Dendrobium nobile, 0.5~2.0 part of Radix Angelicae Sinensis, 1.0~1.5 portions of Radix Paeoniae Albas, 1.5~2.0 portions of fingered citrons, 1.0~1.5 portions of roses, 1.0~1.5 parts
Chrysanthemum.
2. a kind of preparation method of compound dendrobium officinale oral solution, which is characterized in that comprising steps of
The dendrobium candidum of corresponding parts by weight, Radix Angelicae Sinensis, Radix Paeoniae Alba, fingered citron, rose and chrysanthemum are soaked in water 0.5~3h by S100,
The water that 8~12 times of amounts are added decocts 2~3 times, and 1~2h, filters off the dregs of a decoction, obtain extracting solution every time；
S200 is concentrated into 2.5~3.5 times of medicinal material amount at 50~70 DEG C, obtains concentrate；
S300 is filtered or is centrifuged in 4~25 DEG C of standing 4h or more, addition corrigent and preservative, stirring and dissolving, constant volume, encapsulating,
Sterilizing.
3. preparation method according to claim 2, which is characterized in that after concentrate is made in step S200, flocculation is added
Agent, the flocculant are selected from one of chitosan, gelatin, sodium alginate, xanthan gum, pectin or a variety of.
4. preparation method according to claim 3, which is characterized in that the step S100 specifically includes step and uses medicinal material
Water impregnates 1h, and the water measured with 12 times decocts 2 times, first time 1.5h, second of 1.0h, filters off the dregs of a decoction, obtains extracting solution, wherein
Heating extraction temperature is 70~100 DEG C, is subject to and sufficiently flows back.
5. the preparation method according to claim 4, which is characterized in that the step S200 specifically includes step at 60 DEG C
3 times for being concentrated into medicinal material amount with weight by volume basis, relative density are 1.040~1.050, obtain concentrate.
6. preparation method according to claim 5, which is characterized in that the gelatin solution that gelatin flocculant is 8%.
7. preparation method according to claim 6, which is characterized in that the volume ratio of concentrate and gelatin flocculant is 3:1,
It is sufficiently stirred.
8. preparation method according to claim 7, which is characterized in that the step S300 specifically includes step standing, plate
2% mannitol, 2% xylitol, 0.04% potassium sorbate, stirring and dissolving, constant volume, encapsulating, sterilizing is added in frame filtering.
9. preparation method according to claim 8, which is characterized in that the dwell temperature in the step S300 is 4 DEG C.
10. preparation method according to claim 9, which is characterized in that the time of repose in the step S300 is 4h.
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Citations (2)
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| CN104666928A (en) * | 2015-01-29 | 2015-06-03 | 广西浙商投资有限公司 | Preparation method of rapid decoction piece of dendrobium officinale and chrysanthemum |
| CN106723013A (en) * | 2016-11-22 | 2017-05-31 | 张家港昊盛实业投资有限公司 | Dendrobium candidum oral liquid and preparation method thereof |
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|---|---|---|---|---|
| CN104666928A (en) * | 2015-01-29 | 2015-06-03 | 广西浙商投资有限公司 | Preparation method of rapid decoction piece of dendrobium officinale and chrysanthemum |
| CN106723013A (en) * | 2016-11-22 | 2017-05-31 | 张家港昊盛实业投资有限公司 | Dendrobium candidum oral liquid and preparation method thereof |
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| Title |
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| 张家梁: "孙少山和肝法的临床应用", 《天津中医》 * |
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