CN103735710B - Traditional Chinese medicine composition for treating recessive mastitis of dairy cows and preparation method thereof - Google Patents
Traditional Chinese medicine composition for treating recessive mastitis of dairy cows and preparation method thereof Download PDFInfo
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Abstract
The invention discloses a traditional Chinese medicine composition for treating recessive mastitis of dairy cows and a preparation method thereof. The traditional Chinese medicine composition comprises the following raw herbal materials: the seed of cowherb, astragalus membranaceus, Chinese angelica, codonopsis pilosula, incised spleenwort, fructus liquidambaris, babysbreath, uniflower swisscentaury root, radix paeoniae alba, derris eriocarpa how, caulis clematidis armandii, peach grass, delavay schefflera root and common adenostema herb. The preparation method comprises the following steps of: weighing all the raw herbal materials according to parts by weight, crushing the raw herbal materials in a crusher, and sieving through a 80-120-mesh sieve to obtain powder. The traditional Chinese medicine composition for treating recessive mastitis of dairy cows and the preparation method thereof disclosed by the invention have the beneficial effects that the traditional Chinese medicine composition disclosed by the invention has the effects of clearing heat, removing toxicity, eliminating stasis, dispelling swelling and relieving pain; after being taken orally and used externally, the traditional Chinese medicine composition is penetrated into bodies through skin, therefore, the therapeutic effect is increased; the curative effect is improved; the traditional Chinese medicine composition is used for treating internally and externally, and resolves the current problems and eliminates the root causes; the exterior is nourished by the interior; orally taken and externally applied medicines are promoted with each other; the auto-immunity is increased; side effects to dairy cows cannot be generated; the traditional Chinese medicine composition disclosed by the invention is safe and effective.
Description
Technical field
The present invention relates to the beast medicinal preparation containing deriving from plant, animal or mineral component, particularly a kind of Chinese medicine composition being used for the treatment of bovine subclinical mastitis and preparation method thereof.
Background technology
Mastitis is one of modal disease of milch cow, is also to endanger maximum disease to Cow product.Mastitis is that mammary gland tissue is subject to physics, chemistry, microorganism stimulate a kind of inflammatory change occurred.Be characterized in that Ruzhong somatic cell increases, mammary gland tissue generation pathological change, the character quality of breast occurs abnormal.
The harm mastitis average attack rate of latent mammitis is up to 40% ~ 65%, and wherein the average attack rate of clinic mastitis is 2% ~ 3%, and the average attack rate of latent mammitis is 38% ~ 62%.The sick milch cow of mastitis not only milk yield declines, milk products degrading quality, and due to the increase of discarded milk, and the increasing of medical care expenses and eliminating too early etc. causes huge economic loss to dairy.
Although latent mammitis does not have manifest symptom clinically, cause the decline of milk yield and milk-quality.There is larger change in the newborn nutritional labeling of sick cattle, and containing a large amount of invasive organisms, makes milk be easy to become sour rotten, affect again the nutritive value of milk product and the healthy and safe of people.Raise so sick cattle simultaneously and should increase feeding and management cost, also can be transformed into clinic mastitis and stop giving milk time serious, to such an extent as to make milch cow have to eliminate process, cause larger economic loss; Latent mammitis can also cause the infection of reproductive system and ill, and suffers from the milch cow of latent mammitis, post-partum estrus time lengthening, and conception rate declines etc.Latent mammitis is pathogen carrier, can infect other cattle.Most of clinical mastitis originates in latent mammitis, and therefore, taking necessary measure more important than the clinical mastitis of simple therapy to latent mammitis, is also the important content that milk cattle cultivating is managed.
Cause the main cause microorganism reason of bovine subclinical mastitis: cause the main reason of mastitis to be the infection of pathogen, be mostly staphylococcus aureus and streptococcus, other also have escherichia coli, bacillus pyocyaneus, suppurative corynebacterium, necrobacillus etc.These pathogenic bacterias generally invade through papillary duct and cause morbidity, also have plenty of the uterus of falling ill through wound or gastrointestinal tract etc. and invade breast and fall ill.Environmental factors: as many in cowshed dust, unclean, do not sterilize; Cattle bed is moist, rinses not in time again, sterilizes; Sports ground is muddy, straw in a mattress is unclean; Milch cow sanitary condition is poor, and breast is by mud scholar, fecal pollution; Do not wash one's hands before milking, do not clean nipple, during scouring breast, a lot of cattle cleaned by several cattle one barrel of water, one piece of towel; Vacuum pump actuator is unclean, or the rubber tube on milker is infrequently changed, or cleaning milker does not add any disinfectant; The sick cattle Excreta of mastitis is arbitrarily drained, three milk is arbitrarily extruded on cattle bed, contaminated environment etc.Traumatic factor: various wound and Milking Methods And Techniques is unskilled or misoperation, as excessive in milking machine negative pressure, milk middle twitch overlong time, excessive velocities, even when not valve-off, to leave behind by force newborn cup or lead the reasons such as latex dust improper use, and make papillary duct or newborn pond mucosa injury etc., can latent mammitis be caused.Autogenous infection: as diseases such as puerperal septiemia, acute endometritis, tuberculosis, brucellosis, gastroenteritiss, most generation with the latent mammitis of local response.Latent mammitis also can be caused by the transfer of the diseases such as traumatic cervicitis, pyopericarditis, blood vessel embolism internal organs organ pipe.Feeding and management factor: diet nutrient is unbalanced, for high yield cow, the daily ration of high-energy, high protein is conducive to protection and improves milk yield, too increases the load of breast simultaneously, the resistance of body is reduced, thus cause mastitis.Stress: as bad climate (comprising severe cold, heat etc.), scaring, feedstuff moldy metamorphism etc., they can affect the normal physiological function of milch cow to a certain extent, cause mastitis to be fallen ill and increase.Accepted veterinary practice is lack of standardization also can make milk cattle infected mastitis.High parity, older milch cow easily suffer from latent mammitis.The main manifestations of the sick cattle of latent mammitis and diagnosis main manifestations: latent mammitis does not generally show clinical symptoms, but it has distinctive syndrome.As milk yield reduces increasingly, the composition generation significant change contained in milk, histiocyte such as neutrophil, epithelial cell etc. occur in a large number in milk, and can retain the long duration.Milk becomes alkalescence by normal faintly acid, and the quality of breast reduces, and easily becomes sour rotten.Latent mammitis as treated not in time, then may transfer clinical mastitis to.
Summary of the invention
The object of the present invention is to provide Chinese medicine composition being used for the treatment of bovine subclinical mastitis that a kind of instant effect, therapeutic effect are good and side effect is little and preparation method thereof.
In order to realize foregoing invention object, the invention provides a kind of Chinese medicine composition being used for the treatment of bovine subclinical mastitis, described Chinese medicine composition comprises following raw medicinal material: Semen Vaccariae, the Radix Astragali, Radix Angelicae Sinensis, Radix Codonopsis, Herba Asplenii Incisi, Fructus Liquidambaris, Herba Stellariae Neglectae, Radix Rhapontici, Radix Paeoniae Alba, Xin Can, Caulis Clematidis Armandii, purslane speedwell Herba, Radix Schefflerae Delavayi and Herba Adenostemmatis.
In described Chinese medicine composition, the ratio of weight and number of each raw medicinal material is: Semen Vaccariae 20-35 weight portion, Radix Astragali 20-35 weight portion, Radix Angelicae Sinensis 15-30 weight portion, Radix Codonopsis 15-30 weight portion, Herba Asplenii Incisi 20-35 weight portion, Fructus Liquidambaris 15-25 weight portion, Herba Stellariae Neglectae 10-25 weight portion, Radix Rhapontici 10-25 weight portion, Radix Paeoniae Alba 20-35 weight portion, pungent ginseng 10-25 weight portion, Caulis Clematidis Armandii 10-20 weight portion, purslane speedwell Herba 10-20 weight portion, Radix Schefflerae Delavayi 10-25 weight portion and Herba Adenostemmatis 10-25 weight portion.
In described Chinese medicine composition, the ratio of weight and number of each raw medicinal material is: Semen Vaccariae 23-32 weight portion, Radix Astragali 25-30 weight portion, Radix Angelicae Sinensis 19-25 weight portion, Radix Codonopsis 20-25 weight portion, Herba Asplenii Incisi 24-32 weight portion, Fructus Liquidambaris 17-23 weight portion, Herba Stellariae Neglectae 15-20 weight portion, Radix Rhapontici 15-20 weight portion, Radix Paeoniae Alba 26-31 weight portion, pungent ginseng 14-22 weight portion, Caulis Clematidis Armandii 14-18 weight portion, purslane speedwell Herba 13-17 weight portion, Radix Schefflerae Delavayi 15-20 weight portion and Herba Adenostemmatis 15-20 weight portion.
In described Chinese medicine composition, the ratio of weight and number of each raw medicinal material is: Semen Vaccariae 26 weight portion, the Radix Astragali 27 weight portion, Radix Angelicae Sinensis 21 weight portion, Radix Codonopsis 23 weight portion, Herba Asplenii Incisi 29 weight portion, Fructus Liquidambaris 19 weight portion, Herba Stellariae Neglectae 18 weight portion, Radix Rhapontici 18 weight portion, Radix Paeoniae Alba 29 weight portion, pungent ginseng 18 weight portion, Caulis Clematidis Armandii 16 weight portion, purslane speedwell Herba 15 weight portion, Radix Schefflerae Delavayi 17 weight portion and Herba Adenostemmatis 17 weight portion.
Described Chinese medicine composition also comprises: Ganoderma, meal soup, Fructus Jujubae, Fructus Crataegi and Massa Medicata Fermentata.
In described Chinese medicine composition, the ratio of weight and number of each raw medicinal material is: Semen Vaccariae 20-35 weight portion, Radix Astragali 20-35 weight portion, Radix Angelicae Sinensis 15-30 weight portion, Radix Codonopsis 15-30 weight portion, Herba Asplenii Incisi 20-35 weight portion, Fructus Liquidambaris 15-25 weight portion, Herba Stellariae Neglectae 10-25 weight portion, Radix Rhapontici 10-25 weight portion, Radix Paeoniae Alba 20-35 weight portion, pungent ginseng 10-25 weight portion, Caulis Clematidis Armandii 10-20 weight portion, purslane speedwell Herba 10-20 weight portion, Radix Schefflerae Delavayi 10-25 weight portion, Herba Adenostemmatis 10-25 weight portion, Ganoderma 20-30 weight portion, meal soup 10-20 weight portion, Fructus Jujubae 10-20 weight portion, Fructus Crataegi 10-25 weight portion and Massa Medicata Fermentata 10-20 weight portion.
In order to better realize foregoing invention object, present invention also offers a kind of preparation method being used for the treatment of the Chinese medicine composition of bovine subclinical mastitis, the dosage form of described Chinese medicine composition is powder, described preparation method comprises the following steps: take all raw medicinal materials according to ratio of weight and number, input pulverizer is pulverized, cross 80-120 mesh sieve, obtain powder.
In order to better realize foregoing invention object, invention further provides a kind of preparation method being used for the treatment of the Chinese medicine composition of bovine subclinical mastitis, the dosage form of described Chinese medicine composition is described Chinese medicine composition is unguentum, and described preparation method comprises the following steps:
A, by the Radix Astragali, Radix Angelicae Sinensis and Radix Codonopsis ethanol extraction, concentrated, obtain concentrated solution 1;
B, other raw medicinal materials are pulverized after put into ethanol and soak, extract, concentratedly to obtain concentrated solution 2;
C, above-mentioned two kinds of concentrated solutions 1 and concentrated solution 2 to be mixed, mix vaseline or Cera Flava makes unguentum.
In described step a, get the Radix Astragali, Radix Angelicae Sinensis and Radix Codonopsis according to described ratio of weight and number, after soak with ethanol 2-3 hour of 70-80%, 70 ~ 85 DEG C of heat are extracted, and ethanol consumption used is 10 times amount of the Radix Astragali, Radix Angelicae Sinensis and Radix Codonopsis three kinds of raw medicinal material gross weights; The concentrated ethanol extract of heating, is concentrated into the concentrated solution 1 that relative density under room temperature is 1.08, stand for standby use.
In described step b, by raw medicinal material described in all the other by proportioning mixing, drop into pulverizer and be ground into fine powder, acid and alkali-resistance infusion kettle is put into after fine powder sieves, at normal temperatures and pressures, jointly flood 10 ~ 15 days with the ethanol of 70-85%, the liquid flooded and medicinal residues are carried out squeeze and filter, get filtrate after separation, obtain concentrated solution 2.
The beneficial effect that the technical scheme that the embodiment of the present invention provides is brought is: the effect of middle pain relieving that Chinese medicine composition of the present invention has heat-clearing and toxic substances removing, dissipating blood stasis for subsidence of swelling eases up, oral administration and externally used, penetrates in body through skin, strengthens therapeutical effect, improves curative effect; This Chinese medicine composition internal and external application, within support outside, treating both the principal and secondary aspects of a disease, both Orally taken and externally medicines are mutually promoted, and improve autoimmunity, and not to have side effects to milch cow, safe and effective.
Detailed description of the invention
Embodiment 1 powder
Embodiments provide a kind of Chinese medicine composition being used for the treatment of bovine subclinical mastitis, in Chinese medicine composition, the ratio of weight and number of each raw medicinal material is: Semen Vaccariae 26 weight portion, the Radix Astragali 27 weight portion, Radix Angelicae Sinensis 21 weight portion, Radix Codonopsis 23 weight portion, Herba Asplenii Incisi 29 weight portion, Fructus Liquidambaris 19 weight portion, Herba Stellariae Neglectae 18 weight portion, Radix Rhapontici 18 weight portion, Radix Paeoniae Alba 29 weight portion, pungent ginseng 18 weight portion, Caulis Clematidis Armandii 16 weight portion, purslane speedwell Herba 15 weight portion, Radix Schefflerae Delavayi 17 weight portion and Herba Adenostemmatis 17 weight portion.
The dosage form of above-mentioned Chinese medicine composition is powder, and its preparation method comprises the following steps: take all raw medicinal materials according to ratio of weight and number, drops into pulverizer and pulverizes, cross 120 mesh sieves, obtain powder.
Embodiment 2 unguentum
Embodiments provide a kind of Chinese medicine composition being used for the treatment of bovine subclinical mastitis, in Chinese medicine composition, the ratio of weight and number of each raw medicinal material is: Semen Vaccariae 27 weight portion, the Radix Astragali 27 weight portion, Radix Angelicae Sinensis 23 weight portion, Radix Codonopsis 22 weight portion, Herba Asplenii Incisi 27 weight portion, Fructus Liquidambaris 20 weight portion, Herba Stellariae Neglectae 17 weight portion, Radix Rhapontici 17 weight portion, Radix Paeoniae Alba 27 weight portion, pungent ginseng 19 weight portion, Caulis Clematidis Armandii 15 weight portion, purslane speedwell Herba 15 weight portion, Radix Schefflerae Delavayi 18 weight portion, Herba Adenostemmatis 17 weight portion, Ganoderma 25 weight portion, meal soup 15 weight portion, Fructus Jujubae 15 weight portion, Fructus Crataegi 17 weight portion and Massa Medicata Fermentata 15 weight portion.
The dosage form of above-mentioned Chinese medicine composition is unguentum, and its preparation method comprises the following steps:
A, by the Radix Astragali, Radix Angelicae Sinensis and Radix Codonopsis ethanol extraction, concentrated, obtain concentrated solution 1;
B, other raw medicinal materials are pulverized after put into ethanol and soak, extract, concentratedly to obtain concentrated solution 2;
C, above-mentioned two kinds of concentrated solutions 1 and concentrated solution 2 are mixed, the vaseline or the Cera Flava that mix 85% of concentrated solution 1 and concentrated solution 2 mixed weight make unguentum.
Wherein, in step a, get the Radix Astragali, Radix Angelicae Sinensis and Radix Codonopsis according to described ratio of weight and number, the soak with ethanol with 75% is after 3 hours, and 80 DEG C of heat are extracted, and ethanol consumption used is 10 times amount of the Radix Astragali, Radix Angelicae Sinensis and Radix Codonopsis three kinds of raw medicinal material gross weights; The concentrated ethanol extract of heating, is concentrated into the concentrated solution 1 that relative density under room temperature is 1.08, stand for standby use.
In step b, by all the other raw medicinal materials by proportioning mixing, drop into pulverizer and be ground into fine powder, put into acid and alkali-resistance infusion kettle after fine powder sieves, at normal temperatures and pressures, jointly flood 12 days with the ethanol of 80%, the liquid flooded and medicinal residues are carried out squeeze and filter, after separation, gets filtrate, obtain concentrated solution 2.
Embodiment 3 powder
Embodiments provide a kind of Chinese medicine composition being used for the treatment of bovine subclinical mastitis, in Chinese medicine composition, the ratio of weight and number of each raw medicinal material is: Semen Vaccariae 35 weight portion, the Radix Astragali 35 weight portion, Radix Angelicae Sinensis 15 weight portion, Radix Codonopsis 30 weight portion, Herba Asplenii Incisi 20 weight portion, Fructus Liquidambaris 15 weight portion, Herba Stellariae Neglectae 10 weight portion, Radix Rhapontici 25 weight portion, Radix Paeoniae Alba 35 weight portion, pungent ginseng 10 weight portion, Caulis Clematidis Armandii 20 weight portion, purslane speedwell Herba 10 weight portion, Radix Schefflerae Delavayi 25 weight portion, Herba Adenostemmatis 10 weight portion, Ganoderma 25 weight portion, meal soup 10 weight portion, Fructus Jujubae 15 weight portion, Fructus Crataegi 10 weight portion and Massa Medicata Fermentata 20 weight portion.
The dosage form of above-mentioned Chinese medicine composition is powder, and its preparation method comprises the following steps: take all raw medicinal materials according to ratio of weight and number, drops into pulverizer and pulverizes, cross 100 mesh sieves, obtain powder.
Wherein, the pharmacology of each raw medicinal material is as follows:
Semen Vaccariae: bitter is flat.Return liver, stomach warp.Function cures mainly: promoting blood circulation to restore menstrual flow, and stimulating milk secretion is subsided a swelling.For agalactia, amenorrhea, dysmenorrhea, acute mastitis swells and ache.
The Radix Astragali: sweet, tepor; Return lung, spleen, liver, kidney channel.Admittedly de-for benefiting QI for strengthening the superficies, arresting sweating, to hold in the palm skin ulcer granulation promoting, inducing diuresis to remove edema effect." book on Chinese herbal medicine meets former " carries: all void of Radix Astragali energy tonifying five ZANG-organs, controls stringy pulse spontaneous perspiration, rushes down YIN-fire, remove lung-heat, lossless, sends out, has antiperspirant then to stop.Be used for the treatment of the deficiency of vital energy weak, sinking of QI of middle-JIAO, chronic diarrhea proctoptosis, metrorrhagia of having blood in stool, exterior deficiency spontaneous perspiration, carbuncle difficulty is burst, and burst for a long time and do not hold back, blood deficiency and yellow complexion, interior-heat is quenched one's thirst, chronic nephritis, albuminuria, diabetes etc.The invigorating middle warmer of Radix Astragali Preparata QI invigorating, raw by consolidating superficial resistance holder skin ulcer.The Radix Astragali is the good medicine promoting resistance and defence disease.
Radix Angelicae Sinensis: sweet in the mouth and weighing, therefore specially can enrich blood, its gas is light and pungent, therefore again can promoting the circulation of blood, and invigorating middle warmer has, has benefit, the gas medicine in sincere blood, the panacea also in blood in row.Then mending to mend of about assistant, therefore can nutrition blood be supported, QI invigorating spermatogenesis, settling five organs, strong body, beneficial mind, all tangible deficient diseases, unsuitable without institute.Assistant to attack, lead to, therefore pain relieving constipation of dispelling, relieving rigidity of muscles and joints, controls contracture, paralysis, the card such as dry, puckery.Nutrient QI being deficient and exterior syndrome being not relieved person, help with agent such as bavin, Pueraria lobota, fiber crops, osmanthus, and can fall apart greatly Biao Weire, and show not hold back person, helps with Radix Et Rhizoma Rhei and so on, again can consolidating superficial resistance.Precisely because gas Xin Erdong, therefore for its quiet person when keeping away it, the sliding benefaction of property, stool does not consolidate person when keeping away it.All cloudy moderate heat Sheng persons, the active blood of Radix Angelicae Sinensis, also non-institute is suitable, and cloudy deficiency of YANG of middle-JIAO person, Radix Angelicae Sinensis can nourish blood, and is indispensable.
Radix Codonopsis: property is put down, sweet in the mouth, micro-acid.Return spleen, lung meridian.Invigorating the spleen and replenishing QI, spleen invigorating lung benefiting.For deficiency of the spleen and lung, cardiopalmus of breathing hard, anorexia and loose stool, dyspnea due to deficiency is coughed, and interior-heat is quenched one's thirst." book on Chinese herbal medicine justice " carries: Radix Codonopsis power energy spleen reinforcing nourishing the stomach, the lung moistening and production of body fluid promoting, and gas in tendon fortune, this is very mutually not far away with Radix Ginseng.Its outstanding valuable person, then spleen invigorating fortune and not dry, grow stomach-Yin and not wetting, lung moistening and do not violate cold and cool, nourishes blood and partially do not grow greasy, inspiring clear sun, gas in vibration, and without just dry fraud.Radix Astragali QI invigorating, can enriching spleen-QI be risen, again can lung benefiting consolidating superficial resistance.Radix Codonopsis QI invigorating, can only spleen invigorating QI invigorating, and without the power of consolidating superficial resistance, but Radix Codonopsis can also supplementing QI for promoting the production of body fluid, and the Radix Astragali is then without the effect of promoting the production of body fluid, and the Radix Astragali is held concurrently can diuretic, and Radix Codonopsis is without diuretic effect.This two tastes medicine share can invigorating the spleen and replenishing QI, improves resistance.
Herba Asplenii Incisi: bitter, micro-sweet, cool.Function cures mainly: heat-clearing and toxic substances removing, cough-relieving sore-throat relieving, inducing diuresis and reducing edema, hemostasis and pain-relieving.For influenza, flu, cough, tonsillitis, parotitis, conjunctival congestion and swelling pain, enteritis, dysentery, agalactia; External is controlled skin ulcer and to be swollen furunculosis, and traumatic injury, eczema, burn and scald, knife injury is hemorrhage.
Fructus Liquidambaris: bitter is flat.Return liver, kidney channel.Function cures mainly: dispelling, collateral-activating, and diuretic stimulates the menstrual flow.For arthralgia, numbness contracture, edema distension, the few amenorrhea of breast.
Herba Stellariae Neglectae: micro-hardship; Cool in nature.Return stomach; The heart; Liver Channel; Function cures mainly: heat-clearing and toxic substances removing; Treating stranguria; Blood stasis dispelling.Main sick pyogenic infections from tumour or sore; Scall; Mastalgia; Hemorrhoid; Dysentery; Toothache; Pyretic stranguria; Puerperal abdonimal pain.
Radix Rhapontici: bitter, cold.Return stomach warp.Function cures mainly: heat-clearing and toxic substances removing, eliminating carbuncle, stimulating milk secretion, and relaxing muscles and tendons is promoted blood circulation.Swell and ache for acute mastitis, carbuncle carbuncle on the back, scrofula sore, galactostasis, arthralgia chiefly caused by damp pathogen contracture.
Radix Paeoniae Alba: cool in nature, bitter in the mouth acid, is slightly cold, has easing the affected liver of enriching blood, suppressing the hyperactive liver pain relieving.Yin fluid astringing receives the effects such as antiperspirant, is applicable to fever due to yin deficiency, menoxenia, the breast abdomen pain over the hypochondriac region, extremity contraction suddenly, dysentery stomachache, spontaneous sweating, metrorrhagia, the inferior disease of band.Paeoniflorin has antibacterial, antipyretic, antiinflammatory, increase coronary flow, improves the effect of myocardial nutritional flow, blood vessel dilating, antagonism acute myocardial ischemia, anticoagulant, calmness, analgesia, spasmolytic, antiulcer, adjustment blood glucose.Radix Paeoniae Alba decoct can suppress dysentery bacterium, streptococcus pneumoniae, escherichia coli, Bacillus typhi, Hemolytic streptococcus, bacillus pyocyaneus etc.
Xin Can: sweet in the mouth; Bitter; Cool in nature.Function cures mainly: regulating qi-flowing for strengthening spleen; Removing toxic substances and promoting subsidence of swelling.Main dyspepsia; Abdominal distention; Diarrhoea; Cough with asthma breast is vexed; Flu; Laryngopharynx swelling and pain; Drug intoxication; Traumatic injury congestive edema.
Caulis Clematidis Armandii: light, bitter, cold.GUIXIN, lung, small intestinal, urinary bladder channel.Function cures mainly: clearing away heat and promoting diuresis, stimulating milk secretion of stimulating the menstrual flow.For edema, gonorrhea, urinary obstruction, arthralgia, amenorrhea breast is few.
Purslane speedwell Herba: sweet, bitter, temperature.Function cures mainly: invigorate blood circulation, hemostasis, detumescence, pain relieving.For spitting blood, epistaxis, spitting of blood, has blood in stool; Fracture is controlled in external, traumatic injury, and congestion swells and ache.
Ganoderma: property is sweet, flat.GUIXIN, lung, liver, kidney channel.Cure mainly asthenia, cough, asthma, insomnia, dyspepsia, malignant tumor etc.Animal pharmacology shows experiment: Ganoderma has inhibitory action to nervous system, and blood circulation has blood pressure lowering and strengthens the effect of cardiac contractile force, has phlegm-dispelling functions to respiratory system, in addition, also has hepatoprotective, improves immunologic function, antibacterially waits effect.
Radix Schefflerae Delavayi: bitter, puckery, is slightly cold.Function cures mainly: reducing swelling and alleviating pain, expelling wind and removing dampness, relaxing muscles and tendons and activating QI and blood in the collateral, relieving constipation.Root, stem: toothache, stomachache, stomachache, constipation, traumatic injury, closed fracture.Bark: rheumatic numbness, arthralgia, traumatic injury stasis of blood pain, soreness of waist and knee joint, stomachache.Leaf: dermatitis, eczema, rubella.
Herba Adenostemmatis: acrid in the mouth; Micro-hardship; Cool in nature.Function cures mainly: heat-clearing and toxic substances removing; Expelling wind and removing dampness.Main cold, fever; Icterohepatitis; Cough due to lung-heat; Laryngopharynx swelling and pain; Beriberoid pyretic arthralgia; Acute mastitis; Carbuncle furuncle; Venom.
Meal soup: micro-hardship.Function cures mainly: spleen invigorating.Cure mainly weakness of the spleen and stomach, stomach is indigestion and loss of appetite blunt.
Fructus Jujubae: there is immunosuppressive action, the ethanol extract of Fructus Jujubae with every day 100mg to rat oral gavage, display Fructus Jujubae has same immunosuppressant with azathioprine; The generation of antibody can be suppressed to atopic diseases.Fructus Jujubae ethanol extract may have inhibitory action to rat reactive antibody, and other effects of inhibitory action are not produced to non-reacted antibody: the two glucoside A of the flavone in Fructus Jujubae, pharmacological evaluation proves have calmness, hypnosis and hypotensive effect, and Fructus Jujubae has the effect increasing cAMP in leukocyte.Fructus Jujubae decoct can reduce the damage of CCl4 to Rabbit Liver.The swimming time of mice can be extended.Nearest report Fructus Jujubae has the proliferation function of anticancer.
Fructus Crataegi: sour in the mouth, sweet, slightly warm in nature, returns spleen, stomach, Liver Channel, for promoting digestion and invigorating the stomach, circulation of qi promoting dissipating blood stasis, be mainly used to treat accumulation of food in the stomach and intes tine due to indigestion, have loose bowels, dysentery, Fructus Crataegi be the staple for the treatment of dyspepsia, and Fructus Crataegi can increase the secretion of peptic digestion enzyme, facilitating digestion; To dysentery bacterium, escherichia coli, bacillus pyocyaneus, staphylococcus aureus etc., there is obvious inhibitory action.
Massa Medicata Fermentata: be the processed goods through fermentation after whole wheat flour and other drug (Herba Artemisiae Annuae, Herba Xanthii, the buds such as Herba polygoni hydropiperis, Semen Armeniacae Amarum, Semen Phaseoli) mixing.Get Massa Medicata Fermentata to put in pot and fry to appearance be coke black, inner coke yellow, take out, sprinkling disappears water, cools, is Massa Medicata Fermentata (parched to brown).Massa Medicata Fermentata forms through fermentation, and the product of all fermentations have the digestant effect of strengthening the spleen and stomach, and therefore, bad, feeling of stuffiness in chest abdominal distention for the digestion caused by diet internal injury quite have effect.
Toxicity test
1, acute toxicity test for oral administration:
Application NIH mice 40, SPF level, male and female half and half, body weight 16 ~ 20g, carries out acute toxicity test.Mice is divided into two groups at random, often organizes 20, i.e. matched group and administration group, fasting 12 hours before experiment; Powder prepared by embodiments of the invention 1 is dissolved in water, (concentration is 6.58g crude drug/ml, maximum concentration) gavage, gavage volume is 5ml/kg(and unit dosage form is 32.9g crude drug/kg), matched group gives normal saline, administration in a day 2 times, delivery time 6 hours, Continuous Observation 14 days after administration, and record toxic reaction and the death toll of mice.Experimental result shows: compare with matched group, and after administration, mice has no notable difference, and experiment Continuous Observation 14 days, mouse systemic situation, diet, drinking-water, body weight increase all normal.Mouse oral gavage powder LD50>32.9g crude drug/kg of the present invention, every day, maximum dosage-feeding was 65.8g crude drug/kg/ day.Chinese medicine clinical application amount of the present invention be 418g crude drug/day/head, Niu Tichong is in 700kg, and average dosage is 0.597g crude drug/kg/ day.By weighing machine: the dosis tolerata of mice (average weight is in 20g) oral administration gavage Chinese medicine of the present invention is 110 times of quantity.Therefore Chinese medicine composition acute toxicity of the present invention is low, clinical drug safety.
2, long term toxicity test for oral administration:
The powder of the embodiment of the present invention 3 is pressed 11.8,32.9 and 65.8g crude drug/kg/ day continuous use 12 weeks (1.0ml/100g body weight to mice, every day 2 times) and drug withdrawal after 4 weeks, result shows: the index such as hair, behavior, defecation, body weight, organ weights, hemogram, hepatic and renal function, blood glucose, blood fat of Chinese medicine composition of the present invention to rat all has no significant effect, internal organs naked eyes do not find that difference change and histological indications show, medication 12 weeks and drug withdrawal are after 4 weeks, and each internal organs of rat are all without obviously changing.Illustrate that Chinese medicine composition of the present invention is little to toxicity after rat long-term prescription, also there is no difference reaction after drug withdrawal, application safety.
3, external acute skin irritation test:
Get male 4 of Japan large ear rabbit, body weight 2kg ~ 3kg, get the unguentum of the 0.5g embodiment of the present invention 2, directly spread upon on the left of animal on skin (opposite side in contrast), adopt blocking test, 4 hours application time, tested material is remained with warm water washing after off-test, after removing tested material, 1h, 24h, 48h and 72h observe the dermoreaction of the tested district of animal and check plot respectively, and top average is " 0 ", judges that these product are to animal skin nonirritant.
Clinical trial
(1) material prepares---and test milch cow and place are chosen
Select Jinan, Shandong Province city dairy cow farm to be Experimental Base, with the milch cow of 700kg for experimental subject, start in June, 2012 to add up the milch cow quantity suffering from mastitis, to during in May, 2013 totally 369.
(2) diagnostic method: adopt milk pH to measure and Ruzhong somatic cell inspection.Milk pH measures: adopt the test of bromine Moschus oxalic acid indigo plant, main agents is the ethanol 500 milliliters of 47%, blue 1.0 grams of bromine Moschus oxalic acid, 5%NaOH1.3 milliliter ~ 1.5 milliliter, stirring and evenly mixing, and in green, pH is 7.0.Get tested newborn 5 milliliters, reagent adding 1 milliliter, mixing, observe color and judge.Yellow green (below pH6.5) is normal breast, and green (pH6.6), for suspicious, blue is positive to dark green (more than pH6.6).Ruzhong somatic cell checks: judge whether milch cow suffers from latent mammitis according to the somatic number in Ruzhong, and somatic number is negative at every milliliter less than 200,000; Somatic number just likely suffers from latent mammitis every milliliter 200,000 ~ 500,000, and somatic number suffers from latent mammitis or clinic mastitis certainly at every milliliter more than 500,000.
(2) contrast test
Select ill milch cow 360 to take different treatment meanss to cure respectively as test milch cow, 360 cow heads are divided into four groups at random, often organize 90, are respectively treatment group 1-3 and matched group; Therapeutic Method is respectively: treatment group 1 adopts the powder of embodiment 1 and the unguentum partner treatment of embodiment 2; Treatment group 2 adopts the unguentum of embodiment 2 and the powder partner treatment of embodiment 3; Treatment group 3 adopts the powder partner treatment of embodiment 3; Matched group adopts neck injection penicillin, and once, inject 5-7 days continuously, and gavage Chinese medicine " GONGYINGSAN ", every day 1 time, each 250g, takes 1 week continuously in injection in two days.Wherein, the using method of the powder of embodiment 1 and embodiment 2 is for take with warm water, and each 300g, once a day, every day smeared breast three times by the unguentum of embodiment 2, treats one week.
(3) therapeutic outcome
After treatment terminates, adopt milk pH to measure and detect.Testing result is that the positive is judged to be recovery from illness.Therapeutic outcome synopsis is as shown in table 1.
Table 1 therapeutic effect synopsis
| Group | Treatment group 1 | Treatment group 2 | Treatment group 3 | Matched group |
| Cure rate | 95.56% | 97.78% | 92.22% | 77.78% |
From the data of table 1, the bovine subclinical mastitis of the treatment for the treatment of group 1-3 is obviously better than matched group 1.After the treatment of a week, carry out following the tracks of half a year and understand, find that the milch cow of fully recovering in matched group is easily recurred, and the milch cow of recurrence in matched group produces drug resistance; Treatments period, observe and find, treatment group 1-3 cow feeding amount slightly increases compared with before ill, and matched group feed intake slightly declines.
The foregoing is only preferred embodiment of the present invention, not in order to limit the present invention, within the spirit and principles in the present invention all, any amendment done, equivalent replacement, improvement etc., all should be included within protection scope of the present invention.
Claims (8)
1. be used for the treatment of the Chinese medicine composition of bovine subclinical mastitis, it is characterized in that, described Chinese medicine composition is made up of the raw medicinal material of following parts by weight: Semen Vaccariae 20-35 weight portion, Radix Astragali 20-35 weight portion, Radix Angelicae Sinensis 15-30 weight portion, Radix Codonopsis 15-30 weight portion, Herba Asplenii Incisi 20-35 weight portion, Fructus Liquidambaris 15-25 weight portion, Herba Stellariae Neglectae 10-25 weight portion, Radix Rhapontici 10-25 weight portion, Radix Paeoniae Alba 20-35 weight portion, pungent ginseng 10-25 weight portion, Caulis Clematidis Armandii 10-20 weight portion, purslane speedwell Herba 10-20 weight portion, Radix Schefflerae Delavayi 10-25 weight portion and Herba Adenostemmatis 10-25 weight portion.
2. the Chinese medicine composition being used for the treatment of bovine subclinical mastitis according to claim 1, it is characterized in that, described Chinese medicine composition is made up of the raw medicinal material of following parts by weight: Semen Vaccariae 23-32 weight portion, Radix Astragali 25-30 weight portion, Radix Angelicae Sinensis 19-25 weight portion, Radix Codonopsis 20-25 weight portion, Herba Asplenii Incisi 24-32 weight portion, Fructus Liquidambaris 17-23 weight portion, Herba Stellariae Neglectae 15-20 weight portion, Radix Rhapontici 15-20 weight portion, Radix Paeoniae Alba 26-31 weight portion, pungent ginseng 14-22 weight portion, Caulis Clematidis Armandii 14-18 weight portion, purslane speedwell Herba 13-17 weight portion, Radix Schefflerae Delavayi 15-20 weight portion and Herba Adenostemmatis 15-20 weight portion.
3. the Chinese medicine composition being used for the treatment of bovine subclinical mastitis according to claim 1, it is characterized in that, described Chinese medicine composition is made up of the raw medicinal material of following parts by weight: Semen Vaccariae 26 weight portion, the Radix Astragali 27 weight portion, Radix Angelicae Sinensis 21 weight portion, Radix Codonopsis 23 weight portion, Herba Asplenii Incisi 29 weight portion, Fructus Liquidambaris 19 weight portion, Herba Stellariae Neglectae 18 weight portion, Radix Rhapontici 18 weight portion, Radix Paeoniae Alba 29 weight portion, pungent ginseng 18 weight portion, Caulis Clematidis Armandii 16 weight portion, purslane speedwell Herba 15 weight portion, Radix Schefflerae Delavayi 17 weight portion and Herba Adenostemmatis 17 weight portion.
4. be used for the treatment of the Chinese medicine composition of bovine subclinical mastitis, it is characterized in that, described Chinese medicine composition is made up of the raw medicinal material of following parts by weight: Semen Vaccariae 20-35 weight portion, Radix Astragali 20-35 weight portion, Radix Angelicae Sinensis 15-30 weight portion, Radix Codonopsis 15-30 weight portion, Herba Asplenii Incisi 20-35 weight portion, Fructus Liquidambaris 15-25 weight portion, Herba Stellariae Neglectae 10-25 weight portion, Radix Rhapontici 10-25 weight portion, Radix Paeoniae Alba 20-35 weight portion, pungent ginseng 10-25 weight portion, Caulis Clematidis Armandii 10-20 weight portion, purslane speedwell Herba 10-20 weight portion, Radix Schefflerae Delavayi 10-25 weight portion, Herba Adenostemmatis 10-25 weight portion, Ganoderma 20-30 weight portion, meal soup 10-20 weight portion, Fructus Jujubae 10-20 weight portion, Fructus Crataegi 10-25 weight portion and Massa Medicata Fermentata 10-20 weight portion.
5. the preparation method being used for the treatment of the Chinese medicine composition of bovine subclinical mastitis as described in any one of claim 1-4, it is characterized in that, the dosage form of described Chinese medicine composition is powder, described preparation method comprises the following steps: take all raw medicinal materials according to ratio of weight and number, input pulverizer is pulverized, cross 80-120 mesh sieve, obtain powder.
6. the preparation method being used for the treatment of the Chinese medicine composition of bovine subclinical mastitis as described in any one of claim 1-4, is characterized in that, the dosage form of described Chinese medicine composition is unguentum, and described preparation method comprises the following steps:
A, by the Radix Astragali, Radix Angelicae Sinensis and Radix Codonopsis ethanol extraction, concentrated, obtain concentrated solution 1;
B, other raw medicinal materials are pulverized after put into ethanol and soak, extract, concentratedly to obtain concentrated solution 2;
C, above-mentioned two kinds of concentrated solutions 1 and concentrated solution 2 to be mixed, mix vaseline or Cera Flava makes unguentum.
7. the preparation method being used for the treatment of the Chinese medicine composition of bovine subclinical mastitis according to claim 6, it is characterized in that, in described step a, the Radix Astragali, Radix Angelicae Sinensis and Radix Codonopsis is got according to described ratio of weight and number, after soak with ethanol 2-3 hour of 70-80%, 70 ~ 85 DEG C of heat are extracted, and ethanol consumption used is 10 times amount of the Radix Astragali, Radix Angelicae Sinensis and Radix Codonopsis three kinds of raw medicinal material gross weights; The concentrated ethanol extract of heating, is concentrated into the concentrated solution 1 that relative density under room temperature is 1.08, stand for standby use.
8. the preparation method being used for the treatment of the Chinese medicine composition of bovine subclinical mastitis according to claim 6, it is characterized in that, in described step b, by raw medicinal material described in all the other by proportioning mixing, drop into pulverizer and be ground into fine powder, acid and alkali-resistance infusion kettle is put into after fine powder sieves, at normal temperatures and pressures, jointly flood 10 ~ 15 days with the ethanol of 70-85%, the liquid flooded and medicinal residues are carried out squeeze and filter, get filtrate after separation, obtain concentrated solution 2.
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| CN104304809B (en) * | 2014-11-09 | 2016-08-17 | 蒋法成 | Milk cow in lactation period feed additive and application thereof |
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| RU2582269C1 (en) * | 2015-03-04 | 2016-04-20 | Анатолий Николаевич Иванников | Method of treating mastitis in cattle |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101011491A (en) * | 2007-01-24 | 2007-08-08 | 辽宁好护士药业（集团）有限责任公司 | Granule for expelling obstruction of galactophore and its preparation method |
| CN102151325A (en) * | 2011-04-13 | 2011-08-17 | 苑学 | Traditional Chinese medicine for treating breast diseases |
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101011491A (en) * | 2007-01-24 | 2007-08-08 | 辽宁好护士药业（集团）有限责任公司 | Granule for expelling obstruction of galactophore and its preparation method |
| CN102151325A (en) * | 2011-04-13 | 2011-08-17 | 苑学 | Traditional Chinese medicine for treating breast diseases |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN104304809B (en) * | 2014-11-09 | 2016-08-17 | 蒋法成 | Milk cow in lactation period feed additive and application thereof |
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