CN109220536A - A kind of inoculation method of organic selenium-rich Cordyceps militaris - Google Patents
A kind of inoculation method of organic selenium-rich Cordyceps militaris Download PDFInfo
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- CN109220536A CN109220536A CN201811381706.1A CN201811381706A CN109220536A CN 109220536 A CN109220536 A CN 109220536A CN 201811381706 A CN201811381706 A CN 201811381706A CN 109220536 A CN109220536 A CN 109220536A
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- cordyceps militaris
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- organic selenium
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G18/00—Cultivation of mushrooms
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G18/00—Cultivation of mushrooms
- A01G18/20—Culture media, e.g. compost
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G18/00—Cultivation of mushrooms
- A01G18/40—Cultivation of spawn
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- Mycology (AREA)
- Environmental Sciences (AREA)
- Mushroom Cultivation (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Abstract
The invention discloses a kind of inoculation methods of organic selenium-rich Cordyceps militaris, organic selenium is enriched in mycelium by the way of Cordyceps militaris spawn fermentation, again by way of adding selenium-enriched crop in solid medium, fruiting bodies of cordyceps militaris can be made maximumlly to be enriched with organic selenium during the growth process.This patent method can be to avoid directlying adopt the problem of sodium selenite causes security risk as raw material, the present invention is by the way of dual enrichment organic selenium simultaneously, the organic selenium content being enriched in resulting fruiting bodies of cordyceps militaris is very high, is conducive to improve Competitive Products.
Description
Technical field
The present invention relates to a kind of selenium-rich culture technology field, specially a kind of inoculation method of organic selenium-rich Cordyceps militaris.
Background technique
Cordyceps militaris is because of the bioactive substances such as its cordycepin rich in, cordycepic acid, Cordyceps sinensis polysaccharide, SOD, medicinal valence
It is worth highly significant.Cordyceps sinensis has good effect in terms of protect liver, guarantor's kidney, moistening lung, while having anticancer, antiviral, removing certainly
By the pharmacological action of base.Distinctive cordycepin in Cordyceps militaris, by NCI (National Cancer research institute) and boston, U.S.A
Three phases of the university for anti-leukocythemia, cancer are clinical.
Selenium is the microelement of human body indispensability, and selenium deficiency will lead to immunity degradation, causes Keshan disease, Kaschin-Beck disease, heart and brain
Vascular diseases, while selenium deficiency not high, the symptoms such as easy aging of skin that also result in sperm motility.Selenium is two in nature existing way
Kind: inorganic selenium and organic selenium.Inorganic selenium refers generally to sodium selenite and sodium selenate, and the latter is that selenium element passes through bioconversion and amino
Acid is combined into.Public reporting selenium element only forms organic selenium in conjunction with amino acid, polysaccharide at present.Selenium propylhomoserin, selenium polysaccharide are not only
It can be used as immunomodulator, be also used as anticancer, antisenescence health product, skin care item etc..
Organic selenium-rich Cordyceps militaris is to convert selenium using Cordyceps militaris as the carrier of selenium-rich by hyphal cell metabolic pathway and tie
It closes on Large molecule active substance, forms selenium propylhomoserin, selenoprotein and selenium polysaccharide.At present the selenium-enriched cordceps militaris of public reporting be all
Sodium selenite is added in culture medium, and sodium selenite is not absolutely by fruiting bodies of cordyceps militaris after hyphal cell is metabolized
It is absorbed into organic selenium, but it is more to form selenium propylhomoserin (mainly with the presence of selenomethionine, selenocysteine), selenium
Sugar, the free many kinds of substance such as selenium and a small amount of sodium selenite, and sodium selenite belongs to extremely toxic substance, long-term consumption will cause people
The murder by poisoning of body, therefore there are huge security risks for the mode of this direct addition sodium selenite.
Summary of the invention
The purpose of the present invention is to provide a kind of inoculation methods of organic selenium-rich Cordyceps militaris, using vegetable active selenium and ferment
Female selenium, can be to avoid directlying adopt the problem of sodium selenite causes security risk as raw material as raw material, while the present invention adopts
With the mode of dual enrichment organic selenium, the organic selenium content being enriched in resulting fruiting bodies of cordyceps militaris is very high, is conducive to improve
Competitive Products.
To achieve the above object, the invention provides the following technical scheme:
A kind of inoculation method of organic selenium-rich Cordyceps militaris, the specific steps are as follows:
(1) it configures nutrient solution: pressing glucose 2%, peptone 0.5%, beef extract 0.5%, epsom salt 0.1%, phosphorus
Acid dihydride potassium 0.1%, vitamin b10.001%, vitamin b120.001%, the proportional arrangement fermentor nutrition of water 96.798%
Liquid；
It (2) is 2.04 × 10 by organic selenium content3The Se-enriched yeast powder of mg/kg is added in fermentor nutrient solution, according to
The ratio addition of every 10L fermentor nutrient solution addition 1g Se-enriched yeast powder；
(3) 121 DEG C are set by fermentation jar temperature, autoclave sterilization 30 minutes, then be cooled to room temperature；
(4) Cordyceps militaris strain is aseptically seeded in fermentor in the ratio of 1:1000, the pupa worm
Careless liquid spawn derives from the strain library of Changde Yandi Biotechnology Co., Ltd.；
(5) 19~21 DEG C are set by fermentation jar temperature, ventilation culture 4~5 days；
(6) the good Cordyceps militaris strain of fermentation tank culture in step (5) is applied into rose-bengal plating medium and adds richness
PDA culture medium detects whether the living contaminants of bacterium, fungi respectively；
(7) configure solid medium: taking the nutrient solution in selenium-enriched crop seed and step (1) according to weight ratio is 4:5
Ratio mixed configuration solid medium；The selenium-enriched crop seed source is in Changde Yandi Biotechnology Co., Ltd.；
(8) solid medium is heated to 102 DEG C, sterilized 6 hours under normal pressure, it is cooling stand-by；
(9) step (6) are detected free of contamination Cordyceps militaris strain according to strain: culture medium=2:25 ratio is inoculated with
Into the solid medium cooled down, it is transferred to darkroom；
(10) it is arranged 17~19 DEG C of darkroom temperature, humidity 70~85% is cultivated 4~5 days, is transferred to light after mycelia hair is full
According to cultivation workshop；
(11) 19~21 DEG C of workshop temperature, 200~500Lux of intensity of illumination, humidity 65~85%, training are cultivated in setting illumination
Supporting 45~55 days can harvest.
In the step (7) selenium-enriched crop seed be Se-rich wheat, selenium-rich rice, selenium-rich corn, in selenium-rich soybean
It is one or more.
Compared with prior art, the present invention have it is following the utility model has the advantages that
(1) present invention is enriched with organic selenium by the way of Cordyceps militaris spawn fermentation in mycelium, then by training in solid
The mode for adding selenium-enriched crop in base is supported, fruiting bodies of cordyceps militaris can be made maximumlly to be enriched with during the growth process organic
Selenium.
(2) present invention can solve using vegetable active selenium and yeast selenium as selenium source using sodium selenite as raw material
Bring food-safety problem.
Specific embodiment
Combined with specific embodiments below, technical scheme in the embodiment of the invention is clearly and completely described, shows
So, described embodiments are only a part of the embodiments of the present invention, instead of all the embodiments.Based on the reality in the present invention
Example is applied, all other embodiment obtained by those of ordinary skill in the art without making creative efforts all belongs to
In the scope of protection of the invention.
Embodiment 1:
A kind of inoculation method of organic selenium-rich Cordyceps militaris, the specific steps are as follows:
(1) it configures nutrient solution: pressing glucose 2000g, peptone 500g, beef extract 500g, epsom salt 100g, phosphoric acid
Potassium dihydrogen 100g, vitamin b11g, vitamin b121g, water 96.798L configure fermentor nutrient solution；
It (2) is 2.04 × 10 by organic selenium content3The Se-enriched yeast powder 10g of mg/kg is added in fermentor nutrient solution；
(3) 121 DEG C are set by fermentation jar temperature, autoclave sterilization 30 minutes, then be cooled to room temperature；
(4) the Cordyceps militaris strain of 100ml is aseptically inoculated with into fermentor, the Cordyceps militaris strain
From the strain library of Changde Yandi Biotechnology Co., Ltd.；
(5) 19~21 DEG C are set by fermentation jar temperature, ventilation culture 4~5 days；
(6) the good Cordyceps militaris strain of fermentation tank culture in step (5) is applied into rose-bengal plating medium and adds richness
PDA culture medium detects whether the living contaminants of bacterium, fungi respectively；
(7) it configures solid medium: the nutrient solution 500g mixed configuration solid in Se-rich wheat 400g and step (1) being taken to train
Support base；The Se-rich wheat derives from Changde Yandi Biotechnology Co., Ltd.；
(8) solid medium is heated to 102 DEG C, sterilized 6 hours under normal pressure, it is cooling stand-by；
(9) step (6) are detected free of contamination Cordyceps militaris strain according to strain: culture medium=2:25 ratio is inoculated with
Into the solid medium cooled down, it is transferred to darkroom；
(10) it is arranged 17~19 DEG C of darkroom temperature, humidity 70~85% is cultivated 4~5 days, is transferred to light after mycelia hair is full
According to cultivation workshop；
(11) 19~21 DEG C of workshop temperature, 200~500Lux of intensity of illumination, humidity 65~85%, training are cultivated in setting illumination
Supporting 45~55 days can harvest.
Embodiment 2:
A kind of inoculation method of organic selenium-rich Cordyceps militaris, the specific steps are as follows:
(1) it configures nutrient solution: pressing glucose 2000g, peptone 500g, beef extract 500g, epsom salt 100g, phosphoric acid
Potassium dihydrogen 100g, vitamin b11g, vitamin b121g, water 96.798L configure fermentor nutrient solution；
It (2) is 2.04 × 10 by organic selenium content3The Se-enriched yeast powder 10g of mg/kg is added in fermentor nutrient solution；
(3) 121 DEG C are set by fermentation jar temperature, autoclave sterilization 30 minutes, then be cooled to room temperature；
(4) the Cordyceps militaris strain of 100ml is aseptically inoculated with into fermentor, the Cordyceps militaris strain
From the strain library of Changde Yandi Biotechnology Co., Ltd.；
(5) 19~21 DEG C are set by fermentation jar temperature, ventilation culture 4~5 days；
(6) the good Cordyceps militaris strain of fermentation tank culture in step (5) is applied into rose-bengal plating medium and adds richness
PDA culture medium detects whether the living contaminants of bacterium, fungi respectively；
(7) it configures solid medium: the nutrient solution 500g mixed configuration solid in selenium-rich rice 400g and step (1) being taken to train
Support base；The selenium-rich rice derives from Changde Yandi Biotechnology Co., Ltd.；
(8) solid medium is heated to 102 DEG C, sterilized 6 hours under normal pressure, it is cooling stand-by；
(9) step (6) are detected free of contamination Cordyceps militaris strain according to strain: culture medium=2:25 ratio is inoculated with
Into the solid medium cooled down, it is transferred to darkroom；
(10) it is arranged 17~19 DEG C of darkroom temperature, humidity 70~85% is cultivated 4~5 days, is transferred to light after mycelia hair is full
According to cultivation workshop；
(11) 19~21 DEG C of workshop temperature, 200~500Lux of intensity of illumination, humidity 65~85%, training are cultivated in setting illumination
Supporting 45~55 days can harvest.
Embodiment 3:
A kind of inoculation method of organic selenium-rich Cordyceps militaris, the specific steps are as follows:
(1) it configures nutrient solution: pressing glucose 2000g, peptone 500g, beef extract 500g, epsom salt 100g, phosphoric acid
Potassium dihydrogen 100g, vitamin b11g, vitamin b121g, water 96.798L configure fermentor nutrient solution；
It (2) is 2.04 × 10 by organic selenium content3The Se-enriched yeast powder 10g of mg/kg is added in fermentor nutrient solution；
(3) 121 DEG C are set by fermentation jar temperature, autoclave sterilization 30 minutes, then be cooled to room temperature；
(4) the Cordyceps militaris strain of 100ml is aseptically inoculated with into fermentor, the Cordyceps militaris strain
From the strain library of Changde Yandi Biotechnology Co., Ltd.；
(5) 19~21 DEG C are set by fermentation jar temperature, ventilation culture 4~5 days；
(6) the good Cordyceps militaris strain of fermentation tank culture in step (5) is applied into rose-bengal plating medium and adds richness
PDA culture medium detects whether the living contaminants of bacterium, fungi respectively；
(7) it configures solid medium: taking the nutrient solution 500g in Se-rich wheat 200g, selenium-rich corn 200g and step (1)
Mixed configuration solid medium；The Se-rich wheat, selenium-rich corn derive from Changde Yandi Biotechnology Co., Ltd.；
(8) solid medium is heated to 102 DEG C, sterilized 6 hours under normal pressure, it is cooling stand-by；
(9) step (6) are detected free of contamination Cordyceps militaris strain according to strain: culture medium=2:25 ratio is inoculated with
Into the solid medium cooled down, it is transferred to darkroom；
(10) it is arranged 17~19 DEG C of darkroom temperature, humidity 70~85% is cultivated 4~5 days, is transferred to light after mycelia hair is full
According to cultivation workshop；
(11) 19~21 DEG C of workshop temperature, 200~500Lux of intensity of illumination, humidity 65~85%, training are cultivated in setting illumination
Supporting 45~55 days can harvest.
The following table 1 is the Se form distribution and organic selenium of 3 embodiments of this patent and blank control group (not plus any selenium source)
Total content:
Table 1:
Note: N.D. expression is not detected
According to upper 1 result of table it is found that organic selenium is obvious in the fruiting bodies of cordyceps militaris that the inoculation method that the present invention uses obtains
Organic selenium content is equal in the selenium-enriched cordceps militaris fructification for being substantially improved compared with blank control group (not plus any selenium source), and nurturing
Meet the mushroom selenium-rich standard of the newest promulgation in Hunan Province.
In addition, it should be understood that although this specification is described in terms of embodiments, but not each embodiment is only wrapped
Containing an independent technical solution, this description of the specification is merely for the sake of clarity, and those skilled in the art should
It considers the specification as a whole, the technical solutions in the various embodiments may also be suitably combined, forms those skilled in the art
The other embodiment being understood that.
Claims (2)
1. a kind of inoculation method of organic selenium-rich Cordyceps militaris, which is characterized in that specific step is as follows:
(1) it configures nutrient solution: pressing glucose 2%, peptone 0.5%, beef extract 0.5%, epsom salt 0.1%, di(2-ethylhexyl)phosphate
Hydrogen potassium 0.1%, vitamin b10.001%, vitamin b120.001%, the proportional arrangement fermentor nutrient solution of water 96.798%；
It (2) is 2.04 × 10 by organic selenium content3The Se-enriched yeast powder of mg/kg is added in fermentor nutrient solution, according to every 10L
Fermentor nutrient solution adds the ratio addition of 1g Se-enriched yeast powder；
(3) 121 DEG C are set by fermentation jar temperature, autoclave sterilization 30 minutes, then be cooled to room temperature；
(4) Cordyceps militaris strain is aseptically seeded in fermentor in the ratio of 1:1000, the Cordyceps militaris liquid
Body strain source is in the strain library of Changde Yandi Biotechnology Co., Ltd.；
(5) 19~21 DEG C are set by fermentation jar temperature, ventilation culture 4~5 days；
(6) the good Cordyceps militaris strain of fermentation tank culture in step (5) is applied into rose-bengal plating medium and adds richness PDA training
Feeding base detects whether the living contaminants of bacterium, fungi respectively；
(7) it configures solid medium: taking the ratio that the nutrient solution in selenium-enriched crop seed and step (1) is 4:5 according to weight ratio
Example mixed configuration solid medium；The selenium-enriched crop seed source is in Changde Yandi Biotechnology Co., Ltd.；
(8) solid medium is heated to 102 DEG C, sterilized 6 hours under normal pressure, it is cooling stand-by；
(9) step (6) are detected free of contamination Cordyceps militaris strain according to strain: culture medium=2:25 ratio is seeded to cold
In good solid medium, it is transferred to darkroom；
(10) it is arranged 17~19 DEG C of darkroom temperature, humidity 70~85% is cultivated 4~5 days, is transferred to illumination training after mycelia hair is full
Plant workshop；
(11) 19~21 DEG C of workshop temperature, 200~500Lux of intensity of illumination, humidity 65~85%, culture 45 are cultivated in setting illumination
It can harvest within~55 days.
2. a kind of inoculation method of organic selenium-rich Cordyceps militaris according to claim 1, which is characterized in that the step (7)
Middle selenium-enriched crop seed is one of Se-rich wheat, selenium-rich rice, selenium-rich corn, selenium-rich soybean or a variety of.
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110257308A (en) * | 2019-08-05 | 2019-09-20 | 常德炎帝牧源农业发展有限公司 | A kind of cultural method of organic selenium-rich nostoc |
| CN110301299A (en) * | 2019-08-05 | 2019-10-08 | 武汉岁岁丰农业科技开发有限公司 | A method of it preparing Se-enriched yeast and utilizes Se-enriched yeast production liquid spawn plantation selenium-enriched edible mushroom |
| CN111560320A (en) * | 2020-04-28 | 2020-08-21 | 辽宁金硒生物科技开发有限公司 | Preparation method of selenium-rich cordyceps militaris powder |
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| CN101897275A (en) * | 2010-08-13 | 2010-12-01 | 义乌市丹溪药用生物开发研究所 | Selenium-rich cordyceps culturing method |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN110257308A (en) * | 2019-08-05 | 2019-09-20 | 常德炎帝牧源农业发展有限公司 | A kind of cultural method of organic selenium-rich nostoc |
| CN110301299A (en) * | 2019-08-05 | 2019-10-08 | 武汉岁岁丰农业科技开发有限公司 | A method of it preparing Se-enriched yeast and utilizes Se-enriched yeast production liquid spawn plantation selenium-enriched edible mushroom |
| CN111560320A (en) * | 2020-04-28 | 2020-08-21 | 辽宁金硒生物科技开发有限公司 | Preparation method of selenium-rich cordyceps militaris powder |
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