CN109219352B

CN109219352B - Microbial pesticide preparation composition, preparation method and use method thereof

Info

Publication number: CN109219352B
Application number: CN201780026305.1A
Authority: CN
Inventors: 山崎聪信; 前川大辅; 尾崎刚一; 明星亘俊; 寺田壮志
Original assignee: Kumiai Chemical Industry Co Ltd
Current assignee: Kumiai Chemical Industry Co Ltd
Priority date: 2016-04-28
Filing date: 2017-04-17
Publication date: 2021-06-29
Anticipated expiration: 2037-04-17
Also published as: JP6920283B2; AR108253A1; JPWO2017188049A1; KR102455374B1; TWI731069B; CN109219352A; WO2017188049A1; TW201806482A; KR20180137003A

Abstract

[ problem ] to provide: a microbial pesticide preparation composition which can be stored for a long period of time in a microbial pesticide preparation composition in which acetic acid is highly activated as a biopesticide filamentous bacterium which is a pesticide active ingredient having a controlling effect on plant diseases and insect pests and/or weeds and a plant growth regulating effect; a process for the preparation thereof; and a method of controlling pests or weeds based on the use of the composition; plant growth regulation methods, and the like. [ solution ] the above object is achieved by using a pesticidal composition comprising a solid culture of a biopesticide filamentous fungus which comprises 1 or more kinds of solid culture medium selected from the group consisting of a seed of a grain, a husk, a bran and a ground material thereof, and acetic acid.

Description

Microbial pesticide preparation composition, preparation method and use method thereof

Technical Field

The present invention relates to a microbial pesticide preparation composition and the like. More specifically, the present invention relates to: a highly activated microbial pesticide preparation composition which comprises a filamentous fungus as a pesticide active ingredient, said filamentous fungus exhibiting an effect of controlling diseases and pests, weeds, etc., an effect of regulating plant growth, etc., wherein the activity of controlling the filamentous fungus is enhanced by acetic acid, and wherein the filamentous fungus can survive for a long period of time in a state activated with acetic acid, i.e., has excellent storage stability; a process for the preparation thereof; and a method for controlling pests, weeds and the like based on the use of the composition; plant growth regulation methods, and the like.

Background

Pest control of useful plants, weed control and the like are indispensable works for efficient agricultural production, and a large effect is obtained by using a synthetic pesticide for this purpose. However, in recent years, problems of generation of resistant pests and environmental destruction caused by administration of a large amount of synthetic agricultural chemicals have been raised, and it has become an important issue in the agricultural field to efficiently reduce environmental load and to continue agricultural production.

As one of the solutions, microbial pesticides utilizing microbial functions have been proposed, and by using them alone or in combination with synthetic pesticides, effects of reducing environmental load and effects of suppressing the frequency of emergence of drug-resistant pests, drug-resistant weeds, and the like, which are serious problems in synthetic pesticides, have been confirmed.

Among useful microorganisms exhibiting control activity against pests, diseases, weeds, etc. and improving agricultural productivity, there are fungi consisting of tubular cells called hyphae, which are collectively called "biopesticide filamentous fungi". As a technique for effectively using the filamentous fungus as a biopesticide as an agricultural material, there have been reported various studies, and for example, patent document 1 discloses a disease control agent containing a fungus of the genus bassiana as an agricultural active ingredient, patent document 2 discloses a pest control agent or a disease control agent containing a fungus of the genus beauveria, a fungus of the genus metarhizium, a fungus of the genus paecilomyces, an fungus of the genus aspergillus, a fungus of the genus penicillium, and a fungus of the genus trichoderma as an agricultural active ingredient, patent document 3 discloses a disease control agent containing a fungus of the genus verticillium as an agricultural active ingredient, and patent document 4 discloses a weed control agent containing a fungus of the genus deresia as an agricultural active ingredient.

On the other hand, improvement of the control activity and the like has been confirmed by applying the filamentous fungus as a biopesticide simultaneously with acetic acid, and for example, patent document 5 discloses the following cases: by using Trichoderma bacteria in combination with acetic acid, the Trichoderma bacteria have improved control activity against the leaf rot of Japanese lawngrass.

Although the advantageous effects of the combination of the filamentous biopesticide and acetic acid are known, the knowledge of the composition containing the filamentous biopesticide and acetic acid is still limited to the field mixture prepared by mixing randomly in the use scene, and no report has been found so far about a single-package pesticide preparation on the premise that the composition is stored for a considerable period of time from the preparation stage to the distribution for use. In the present invention, in order to distinguish between a pesticidal composition as a mixture on site used immediately after preparation and a pesticidal composition as a pesticidal preparation in a single package of an industrial product to be preserved, only the latter is referred to as "pesticidal preparation composition".

The reason for staying in such a mixed product on site is clear, and this is because acetic acid exerts a bactericidal action on the biopesticide filamentous fungi as an active ingredient of the pesticide. As an attempt, the method comprises the step of adding 1X 10¹¹When acetic acid was added to an aqueous suspension of viable bacteria belonging to Trichoderma asperellides SKT-1 strain of CFU (colloid Forming Unit)/L, all viable bacteria belonging to SKT-1 strain died within 24 hours after the addition. Although a simple mixture of a filamentous fungus as a biopesticide and acetic acid improves the control activity and the like in a very short period of time after mixing, the fungus is dead with time and thus cannot be stored, and it is not established as an industrial product or as a pesticide preparation composition requiring storage stability.

In such background art, there is a strong demand in the industry to develop a preparation technology in which the biopesticide filamentous fungus does not die with time (can be stored for a long period of time) even when the biopesticide filamentous fungus and acetic acid are used in combination.

Documents of the prior art

Patent document

Patent document 1: japanese patent laid-open publication No. 2007-31294;

patent document 2: japanese patent laid-open publication No. 7-48216;

patent document 3: japanese patent laid-open No. 2006-169115;

patent document 4: japanese patent laid-open publication No. 6-277042;

patent document 5: japanese patent laid-open publication No. 9-87122.

Disclosure of Invention

Problems to be solved by the invention

The object of the present invention is to provide: a microbial pesticide preparation composition in which a biopesticide filamentous bacterium as a pesticide active ingredient is highly activated by acetic acid and can be stored for a long period of time; a process for the preparation thereof; and a method for controlling pests, weeds, or the like based on the use of the composition; plant growth regulation methods, and the like.

Means for solving the problems

The present inventors have conducted intensive studies in order to achieve the above object, and as a result, have found that: the present inventors have found that the above-mentioned problems can be solved by a microbial pesticide preparation composition comprising a solid culture of a filamentous biopesticide, which comprises a seed of a grain and/or a processed product of the seed (any one or more of husk, bran and ground matter (polished material)) as a solid medium, and acetic acid, and have completed the present invention.

That is, the embodiments of the present invention are as follows.

(1) A pesticidal composition comprising a solid culture of a biopesticide filamentous fungus using at least one member selected from the group consisting of a seed of a grain, a husk, a bran and a ground product thereof as a solid medium, and acetic acid.

(2) The pesticidal preparation composition according to (1), wherein the biopesticide filamentous fungus is a filamentous fungus having an effect of controlling pests and/or weeds and an effect of regulating plant growth.

(3) The pesticidal preparation composition according to (2), wherein the filamentous fungus having a controlling effect on pests and/or weeds and a plant growth regulating effect is a fungus belonging to any one of the genera Beauveria (Beauveria), Coniothyrium (Conothyronium), Metarhizium (Metarhizium), Talaromyces (Talaromyces), Trichoderma (Trichoderma) and Verticillium.

(4) The pesticidal preparation composition according to (3), wherein the filamentous fungus having an effect of controlling pests and/or weeds and an effect of regulating plant growth is a Trichoderma fungus.

(5) The pesticidal preparation composition according to (4), wherein the Trichoderma bacterium is any one of an Aspergillus sp, a Trichoderma asperellum sp, a Trichoderma atroviride sp, a Trichoderma hamatum sp, a Trichoderma harzianum sp, and a Trichoderma koningii sp.

(6) The pesticidal preparation composition of (5), wherein the Trichoderma is an asperellides strain.

(7) The pesticidal preparation composition according to (6), wherein the Aspergillus sp.sp.is Trichoderma asperelloides SKT-1 strain (FERM BP-16510).

(8) The pesticidal preparation composition according to any one of (1) to (7), wherein: 1g of pesticide preparation composition contains 10-10 g¹⁰A biological pesticide filamentous fungus of CFU (Colony Forming Unit).

(9) The pesticidal formulation composition according to any one of (1) to (8), wherein the solid medium is a ground product of a cereal seed.

(10) The pesticidal preparation composition according to any one of (1) to (9), wherein the cereal is a cereal of the family Poaceae.

(11) The pesticidal preparation composition according to (10), wherein the cereal of Gramineae is barley.

(12) The pesticidal preparation composition according to any one of (1) to (11), wherein the content of acetic acid in the pesticidal preparation composition is 0.01 to 1% by mass, for example, 0.04 to 0.6% by mass.

(13) The pesticidal preparation composition according to any one of (1) to (12), wherein: the pesticide preparation composition is a granular pesticide preparation composition, and the solid culture medium is a granular substance.

(14) The pesticidal preparation composition according to (13), wherein acetic acid is an acetic acid-adsorbed substance formed by adsorbing acetic acid to the fine mineral powder.

(15) The pesticidal preparation composition according to (14), wherein the mineral fine powder is hydrous amorphous silica.

(16) The pesticidal preparation composition according to (14) or (15), wherein the content of an acetic acid-adsorbed substance in the pesticidal preparation composition is 0.5 to 2% by mass.

(17) The preparation method of the pesticide preparation composition is characterized by comprising the following steps: a solid culture of a filamentous biopesticide is obtained by solid culturing a filamentous biopesticide in a solid medium containing at least one member selected from the group consisting of a seed of a grain, a husk, a bran and a ground material of the grain, and acetic acid is added to the culture.

(18) The method of (17), characterized by: the pesticide preparation composition is a granular substance, and the solid culture medium is a granular substance.

(19) The method of (17) or (18), wherein: acetic acid is carried by spraying acetic acid or a solution obtained by diluting acetic acid with water or an organic solvent onto the surface of a solid culture of a biopesticide filamentous bacterium.

(20) The method of (18), characterized by: acetic acid is adsorbed to the mineral fine powder, and the adsorbate is coated on the surface of the solid culture of the biopesticide filamentous bacteria.

(21) The method according to (20), wherein the fine mineral powder is hydrous amorphous silica.

(22) A method for controlling at least one selected from weeds, diseases and pests and/or regulating plant growth, characterized in that: applying the pesticidal composition according to any one of (1) to (16) to a seedling, a seedling or a seed of a plant, and cultivating the plant.

(23) A method for controlling at least one selected from weeds, diseases and pests and/or regulating plant growth, characterized in that: applying the pesticidal formulation composition of any one of (1) to (16) to soil in which a plant is cultivated.

(24) The method of (23), characterized by: every 1m²The soil of (1) is applied with 5 to 1000g, for example, 100 to 1000g of the pesticide preparation composition.

Effects of the invention

According to the present invention, there can be provided: a pesticidal composition (microbial pesticidal preparation composition) in the form of a preparation in which a filamentous biopesticide as a pesticidal active ingredient is highly activated by acetic acid and can be stored for a long period of time without preparation immediately before use on site; a process for the preparation thereof; and a method for controlling pests, weeds and the like based on the use of the composition; plant growth regulation methods, and the like.

Detailed Description

The microbial pesticide preparation composition of the present invention is characterized in that: contains acetic acid and a culture obtained by solid-culturing an agriculturally useful filamentous biopesticide (a mixture of a solid culture of a filamentous biopesticide, that is, a cultured cell of a filamentous biopesticide (including a hypha or a spore) and a solid medium containing a seed and/or a processed product of the seed) and acetic acid.

In the present invention, the "biopesticide filamentous fungus" refers to a filamentous fungus which can improve agricultural productivity by controlling pests such as diseases and pests or weeds, improving soil to make it suitable for farming or the like, promoting or suppressing growth of a plant itself or the like, and is not limited to a filamentous fungus having a registered pesticide or a filamentous fungus showing an effect as a pesticide. Examples thereof include: acremonium, Alternaria, Ambrospira, Arthrobotrys, Aspergillus, Aureobasidium, Beauveria, Ascomyces, Botriosporidium, Botrytis, Chrysosporium, Cladosporium, Chrysosporium, Cladosporium, Cocospora, Coccinium, Micrococcus, Geotrichum, Fusarium, Schizosaccharomyces, Geotrichum, Schizosaccharomyces, Geotrichum, Schizothrima, Geotrichum, Schizothricin, Geotrichum, Schizothrix, Schizosaccharomyces, and Schizosaccharomyces, or Schizosaccharomyces, and Schi, The genus Sphaerotheca (Helicomyces), the genus Sphaerotheca (Helicosporium), the genus Heliscus, the genus Helicosporium, the genus Helioticus (Helminthosporium), the genus Hyaloendron, the genus histoplasma (Hypoplasma), the genus Isaria (Isaria), the genus Lemoniera, the genus Metarhizium (Metarhizium), the genus Microsporum (Microsporium), the genus Candida (Monilia), the genus Monochorium (Monochorium), the genus Mortierella (Mortierella), the genus Mucor (Mucor), the genus Nigrospora (Nigrospora), the genus Nodulispora, the genus Nodulisporium, the genus Nomuraea, the genus Penicillium (Oiospora), the genus Penicillium (Aspergillus, the genus Ractomyces, the genus Penicillium, the genus Ractomyces (Pseudomyces), the genus Ractomyceliophthora (P), the genus Ractomyceliophthora (Thermobiella, the genus Ractomyceliophthora, the genus Rabylonicera (Rabylonicera, the genus Raeupatorium (Rabylonicera), the genus Rabylonicera, the genus Raeudiplodia, the genus Rabylonicera, the genus Rabyloni, Thermophilic bacteria (Thermomyces), Trichophyton (Trichophyton), Trichoderma (Trichoderma), Acremonium (Trichoderma), Triperonospora (Trichoderma), Trichophyton (Tripospermum), Aspergillus (Varicosporium), Verticillium (Verticillium), and the like. In the case of filamentous fungi having an activity of controlling diseases and pests, known biopesticide filamentous fungi can be used as desired, and the present invention is not limited to the above-exemplified fungi, but among them, beauveria, coniothyrium, metarhizium, pachysolen, trichoderma, and verticillium are preferably used, and trichoderma is particularly preferably used. Among the Trichoderma bacteria, suitable examples include: the Trichoderma atroviride strain SKT-1 strain is particularly preferably an Aspergillus oryzae strain, among which Trichoderma reesei (hamatum) strain, Trichoderma harzianum (harzianum) strain, Trichoderma koningii (koningii) strain, Trichoderma reesei (Trichoderma harzianum) strain, Trichoderma reesei (koningii) strain and the like, and among the Aspergillus reesei strains, Trichoderma atroviride SKT-1 strain, which was previously named after 11/10 days in 1997 (9 years), was deposited in the national institute of Industrial science and technology institute (national institute for evaluation of technology, national institute of independent administration and technology, patent organism depositary center), and then deposited in 2017 (29 years), and transferred to the internationally deposited Trichoderma atroviride SKT-1 strain (FERM BP-16510) in 13/2/13/s.

The number of filamentous fungi (viable bacteria and/or spores) in the agricultural chemical preparation composition of the present invention is not particularly limited, but is usually 10 to 10 per 1g of the agricultural chemical preparation composition¹⁰CFU (Colony Forming Unit) or so, preferably 10³～10 ⁹CFU is about 10, more preferably⁵～10 ⁸About CFU. The number of filamentous bacteria in the pesticidal composition can be measured by a dilution plate method. Examples of the medium used in the dilution plate method include: examples of the medium include, but are not limited to, a common agar medium, a standard agar medium, a potato dextrose agar medium, an oatmeal agar medium, a malt extract agar medium, a potato carrot agar medium, a marine starch agar medium, a Czapek-Dox agar medium, a Czapek yeast extract agar medium, a MY20 agar medium, a sabouru glucose agar medium, and a Luria-Bertani agar medium. In the dilution plate method, in order to optimize the medium relative to the measurement target, changes such as addition or deletion of medium components, increase or decrease in the amount, and the like are often performed, and any changes can be made according to the knowledge of those skilled in the art. The conditions such as the culture temperature and the culture period may be set arbitrarily according to the optimum temperature and the colony formation rate of the filamentous biopesticide to be treated.

In the agricultural chemical preparation composition of the present invention, one or more kinds selected from the group consisting of seeds of grains, husks, brans and ground materials thereof are used in the solid medium of the solid culture of the biological agricultural chemical filamentous fungi. In the present invention, "cereal" is used in a broad sense, and examples thereof include: examples of the cereal grains include gramineous grains such as rice (rice), corn (mail), barley (barley), wheat (wheat), rye (rye), oat (oat), wild oat (wild oat), coix (adlay), millet (proso milet), millet (foxtail milet), millet (millet), sorghum (sorghum), finger millet (finger milet), dragon finger millet (panicum), royal millet (pearl milet), bran (teff), fornico (fonio), kodo millet (kodo milet), and wild rice (manchurian wild rice) in manchurian, and further include: soybean (soybean), adzuki bean (azuki bean), mung bean (mung bean), cowpea (cowpea), kidney bean (common bean), lima bean (lima bean), peanut (pea), pea (pea), broad bean (broad bean), lentil (lentil), chickpea (chickpea), safflower bean (runner bean), black bean (black gram), moth bean (moth bean), broad bean (tepay bean), red bean (ricebean), hyacinth bean (hyacinth bean), horse bean (horse bean), banbayla (Bambara grondnut), groundnut (geogaroundnut), pigeon pea (pigeon pea), sword bean (sword bean), dwarf bean (jacaraway), buckwheat (pea), guar (pea), locust bean (pea), amaranth (amaranth), amaranth bean (amaranth), amaranth (amaranth bean (amaranth), amaranth bean (amaranth bean), amaranth bean (amaranth, etc.), amaranth (amaranth bean (amaranth, etc.), amaranth bean (amaranth bean), amaranth bean (amaranth bean), amaranth bean (amaranth, etc.), amaranth bean (amaranth, Grains of grains suspected of being grains such as Chenopodiaceae plants. Any edible seed mainly composed of starch can be used, and the present invention is not limited to the above-exemplified grains, and among them, gramineous or leguminous grains are preferable, and among gramineous or leguminous grains, any one or more of barley, wheat, rice and soybean is particularly preferable. The long-term viability of the filamentous fungus as a biopesticide in the presence of acetic acid is improved by applying one or more selected from the group consisting of seeds of cereals, husks, chaffs and ground materials to a solid medium and applying the solid medium containing the same to a preparation.

The seeds of the grain (including, for example, husks such as brown rice and brown wheat) may be used as they are, but husks (such as husks) themselves produced by husking the seeds, bran produced by milling, milled materials themselves, and a mixture of two or more of these substances may be used. In the present invention, the husk, bran and ground material of the seed may be collectively referred to as "processed seed material". The ground material of the seed substantially includes a material composed of the endosperm of the seed and a material formed by the endosperm together with the germ, but is applicable to the present invention and is not particularly distinguished in the present invention.

Such seeds or seed-processed products of grains can be directly used as solid media, and in the present invention, the seeds and/or seed-processed products of grains are used for solid media to culture the biopesticide filamentous fungi, and the obtained biopesticide filamentous fungi solid culture is directly used as a raw material for the pesticide preparation composition of each medium.

The seeds of cereals and their ground materials are originally granular materials, and the husk and bran of the seeds are originally amorphous materials. These granular materials and amorphous materials may be pulverized into a pulverized material (granular pulverized material, pulverized powder material, etc.) such as finer granular material or powder, and then used. The formulation of the agricultural chemical preparation composition of the present invention is determined by itself according to the shape of the seed and/or the processed product of the seed of the grain to be used, and the form of the granular agricultural chemical preparation composition, the indefinite-shaped agricultural chemical preparation composition, or the powdery agricultural chemical preparation composition can be formed, but the form of the granular agricultural chemical preparation composition is preferable, and particularly the form of the granular product having a particle diameter of 2mm or more is more preferable, and therefore, the form of the granular product is preferable also in the seed and/or the processed product of the seed of the grain, and the ground product of the seed of the grain can be particularly preferably used in the granular product.

The method for culturing the filamentous fungus as a biopesticide in the present invention may be arbitrarily set depending on the type, strain, and the like of the filamentous fungus. Further, as a solid medium (carrier for solid medium) in the culture, seeds and/or processed seed products of the above-mentioned cereals are used. In order to increase the growth efficiency, a carbon source, a nitrogen source, inorganic salts, and the like may be added. The culture temperature and the culture time may be set arbitrarily, and for example, conditions for culturing at 10 to 40 ℃ for 2 to 30 days are shown. In addition, the resulting culture may be appropriately dried.

In addition, acetic acid is added to the pesticidal preparation composition of the present invention in order to activate the activity of the biopesticide filamentous bacterium. The acetic acid promotes the hypha growth of the biological pesticide filamentous fungi, and the activity of the biological pesticide filamentous fungi as a pesticide active ingredient is improved, so that the pesticide preparation composition achieves high control effect. The amount of acetic acid to be added is not particularly limited, and when the amount of acetic acid is too small, a sufficient hypha growth promoting effect cannot be expected, while an extremely excessive amount of acetic acid may exert a bactericidal effect on the biopesticide filamentous fungi in the pesticide preparation composition. The amount of acetic acid to be blended is usually about 0.01 to 1% by mass, preferably 0.03 to 0.8% by mass, and more preferably 0.05 to 0.5% by mass, based on the total amount of the pesticidal composition, in order to expect a sufficient hypha growth promoting effect for the pesticidal filamentous fungi and prevent the pesticidal filamentous fungi from being killed.

An example of a method of incorporating acetic acid into a pesticide preparation composition is shown below, but the present invention is not limited to these methods. First, when the culture medium is a powdery material, acetic acid may be added to a solid culture of a filamentous fungus as a biopesticide and then mixed to homogenize the acetic acid in the powdery pesticide preparation composition. On the other hand, when the culture medium is a granular material, acetic acid may be carried on the surface thereof. As a method for supporting acetic acid on the surface of the granular material, for example, there is a method of spraying acetic acid on the granular material or a solution obtained by diluting acetic acid with water or an organic solvent, but it is preferable to first adsorb acetic acid to mineral fine powder such as hydrous amorphous silica (white carbon black) and then coat the acetic acid-adsorbed material on the surface of the solid medium, and in such a case, the long-term viability of the filamentous biopesticide in the presence of acetic acid is further improved. When acetic acid is supported on the surface of the granular material by using the fine mineral powder, the amount of the fine mineral powder supporting acetic acid to be blended is not particularly limited, and is usually about 0.2 to 3% by mass, preferably 0.5 to 2% by mass, and more preferably 0.8 to 1.5% by mass, based on the total amount of the agricultural chemical preparation composition.

As an example of the method for producing the pesticidal preparation composition of the present invention, the following production methods can be shown: a method for producing a solid culture of a biopesticide filamentous fungus characterized by comprising the steps of applying at least one member selected from the group consisting of seeds of grain, husk, bran and ground product thereof to a culture medium, subjecting the biopesticide filamentous fungus to solid culture to obtain a solid culture of a biopesticide filamentous fungus wherein the cultured biopesticide filamentous fungus is adhered to the solid culture medium, and adding acetic acid to the culture. As examples of more preferable production methods, the following production methods can be shown: a solid culture of a filamentous biopesticide is obtained by solid culturing a filamentous biopesticide in a granular solid medium which is a seed of a grain or a ground product thereof to attach the cultured filamentous biopesticide to the granular solid medium, and then acetic acid is supported on the surface of the culture. As examples of further preferable production methods, the following production methods can be shown: a solid culture of a filamentous biopesticide is obtained by solid culturing a filamentous biopesticide in a granular solid medium which is a seed of a grain or a ground product thereof to attach the cultured filamentous biopesticide to the granular solid medium, and then the surface of the culture is coated with aqueous amorphous silica having acetic acid adsorbed thereto.

The method of applying the pesticidal preparation composition of the present invention can be appropriately selected depending on the type of the plant to be applied, the type of the pest, the place of application, the time of application, the formulation, and the like.

The pesticidal formulation composition of the present invention may be applied directly as it is, or may be applied after being diluted with water, a carrier or the like. The application method may be exemplified by: spreading on plant stems and leaves, spreading on plant roots (plant roots), spreading on soil surface, soil mixing, soil pouring, water surface application, seed coating (dressing), coating, dipping, and the like, but the methods are not limited thereto. The pesticidal preparation composition of the present invention may be applied in combination with other bactericides, insecticides, nematicides, herbicides, plant growth regulators, fertilizers, soil improvement materials, and the like, alternately, or simultaneously as needed, and in this case, the composition may sometimes exhibit more excellent effects.

The application site of the pesticide preparation composition of the present invention can be applied to a seedbed, a farmland, a paddy field, a fruit tree garden, a hydroponic facility, etc. for cultivating plants for agricultural and horticultural use, but is not limited thereto.

The application time of the pesticidal composition of the present invention is not limited to the planting time, and in the case of a nursery, it may be applied at any time of before planting, at the time of planting, or after planting, or in the case of a seedling-raising time, it may be applied at any time of before sowing, at the time of sowing, or after sowing.

The amount of the agricultural chemical preparation composition of the present invention to be applied varies depending on the type of the plant to be applied, the type of the plant pest or weed, the state of the soil, the application period, the planting density, the formulation type, etc., and therefore cannot be defined in general, for example, in the case of the seedling raising period, every 1m²The soil can be about 100-1000 g, and in the case of nursery, the soil can be used for every 1m²The amount of the soil used is about 5 to 1000 g. When the seed (including seed potato, tuber, bulb, corm, etc.) is coated with a coating, the ground material may be used as it is or after diluted with water or the like at about 1 to 100g per 1kg of the seed.

Specific examples of plants to which the pesticidal preparation composition of the present invention is applied include: cereals (e.g., rice, wheat, barley, rye, oats, corn, sorghum, millet, foxtail millet, eleaf millet, finger millet, buckwheat), potatoes (e.g., potato, sweet potato, taro, yam, konjac), beans (e.g., soybean, red bean, kidney bean, pea, broad bean, peanut, cowpea, chickpea, pigeon pea), vegetables (e.g., eggplant, tomato, green pepper, hot pepper, cucumber, melon, watermelon, pumpkin, zucchini, cucurbit, white gourd, balsam pear, cabbage, broccoli, cauliflower, white radish, turnip, cabbage, komatsuna, water spinach, onion, leek, garlic, wild garlic (garlic chicken), asparagus, lettuce, garland, burdock, petunia, carrot, jack celery, parsley, strawberry, spinach, okra, rosewood, mint, ginger, and lotus), fruit trees (e.g., apple, pear, common pear, quince, chinese flowering apple, sweet cherry, peach, plum, apricot, chestnut, walnut, almond, pecan, grape, kiwi, akebia stem, persimmon, fig, pomegranate, raspberry, blackberry, blueberry, cranberry, citrus, loquat, olive, bayberry, mango, guava, avocado, date palm, coconut, banana, pineapple, papaya, passion fruit, acerola cherry), specialty crops (e.g., cotton, flax, juncus, rapeseed, sunflower, sesame, palm oil, sugar beet, sugarcane, tea, coffee, cocoa, hop, tobacco), flowers (e.g., boswellia, morning glory, marigold, garden balsam, star anise, bowled pea, chrysanthemum, carnation, tulip, lily, narcissus, sword-grass, cyclamen, sea weed, pygmy, rose, kale, rose, ylang-ylang, garland-grass (e.g., zoysia tenuissima, korean grasses, zoysia japonica, bermuda grass, tassel grass, fescue, ryegrass, poa annua), trees (e.g., cherry blossom, azalea, oak, beech, cypress, beech), and the like, but are not limited to these examples. In addition, it can be applied to plants to which resistance to diseases and pests, resistance to herbicides, and resistance to environmental stress such as desiccation have been imparted by conventional breeding methods, genetic recombination techniques, and the like.

The pesticidal preparation composition of the present invention is excellent in pest control activity and is effective as a bactericide, a nematicide, an insecticide, a herbicide, a plant growth regulator or a soil conditioner. The effects as a bactericide, nematicide or insecticide are not limited to those directly showing the control effect on pathogenic bacteria, nematodes and pests, and include, for example: based on control of viral diseases of filamentous fungi, nematodes, insects, etc. as vectors, indirect control of inhibition of pest proliferation due to the preponderance of biopesticide filamentous fungi in the soil.

Examples of pathogenic bacteria that can be controlled by the pesticidal preparation composition of the present invention include: filamentous bacteria, actinomycetes, bacteria, viruses, viroids, and the like. Specifically, there may be mentioned: the genus of Ustilago (Ustilago), such as, for example, Ustilago grisea (Ustilago nuda), Tilletia (Tilletia) such as, for example, Tilletia foetida (Tilletia carois), Puccinia (Puccinia) such as, for example, Puccinia graminis (Puccinia recondita), Puccinia (Gymnosphaeromonas) such as, for example, Puccinia pyriformis (Gymnosphaeroides), Puccinia sojae (Phakopsora) such as, for example, Puccinia carotovora (Helicobasidium) such as, for example, Puccinia purpurea (Helicobasidium) such as, Podosporium purpureum (Helicoveromyces) such as, Podosporium sp.sp.sp.such as, such as, Podosporium moniliformis such as, Podosporium griseus such as, Podosporus sp.cinerea such as, Podosporus sp.sp.sp.sp.sp.sp.e such as, Photinia Sclerotium such as, Phosphorocarpus (Rhizoctonia) such as, Phosphonospora Sclerotium such as, Phosphorocarpus (Rhizoctonia) such as, Phosphonospora nivorax such as, Phosphonospora cinerea such as, Phosphorocarpus (Rhizoctonia) such as, Phosphonospora, Phosphorum such as, Phosphonospora, Phosphorocarpus (Rhizoctonia) such as, the genus Septoria (Septoria), for example Chrysanthemum nigrosporium (Septoria chrysosporium), the genus Colletotrichum (Colletotrichum) such as strawberry anthracnose (Colletotrichum gloeosporioides), the genus Pestalotiopsis (Pestalotiopsis) such as Pestalotiopsis theobromae (Pestalotiis longissima), the genus Alternaria such as Potato Charantia (Alternaria solani), the genus Botrytis such as Cucumis sativus (Botrytis cinerea), the genus Cercospora (Cercospora), the genus Phanerochaeta such as Pseudocercospora sativus (Pseudocercospora), the genus Phosphaera such as Pseudocercospora maculata (Cercospora cerealis), the genus Pseudocercospora Cercospora such as Pseudocercospora Cercospora (Pseudocercospora), the genus Pseudocercospora Cercospora cerealis (Pseudocercospora cerealis) such as Pseudocercospora Cercospora cerealis (Pseudocercospora cerealis), the genus Pseudocercospora cerealis (Pseudocercospora cerealis) such as Pseudocercospora purpurea, Pseudocercospora cerealospora cerealis (Pseudocercospora cerealis), the genus Pseudocercospora cerealis (Pseudocercospora cerealis) such as Pseudocercospora purpurea, Pseudocercospora cerevispora cerealis (Pseudocercospora cerealis) such as Pseudocercospora purpur, Such as, for example, brown spot fungus (Corynespora cassiicola), fungi of the genus Mycoleptospora (Mycoleptosporium), such as, for example, Phytophthora solani (Mycoleptospora natrassi), Neurospora (Passalacia), such as, for example, Phytophthora fulva (Passalacia fulva), Curvularia (Curvularia), such as, for example, Oryza sativa (Curvularia spp.), Fusarium (Fusarium), such as, for example, Solanum lycopersicum (Fusarium oxysporum f.sp.), Penicillium (Penicillium), such as, for example, Penicillium viridis (Penicillium digitanum), Pyricularia (Pyricularia), such as, for example, Pyricularia grisea (Pyricularia), such as, Verticillium griseula, such as, Thermomum oryzae (Verticillium), such as, hemimyceliophthora solani (Blakebia), such as, Thielavia Such as strawberry powdery mildew (Sphaerotheca aphanidermatum), powdery mildew (Podosphaera), cucumber powdery mildew (Podosphaera xanthorrhiza), powdery mildew (Leveillus) such as tomato powdery mildew (Leveillus taurica), Chinema aleyrodida (Calonetia) such as soybean Rhizopus (Calonella nigricans), Sphaerotheca intermedia (Diaporthe) such as Citrus nigricans (Diaporthe citri), Sphaerotheca acuminata (Gaeumannomyces) such as Rhizoctonia tritici (Gaeumannomyces tritici), Rhizoctonia solani (Gaeumannomyces graminis), Rhizoctonia solani (Rosellinia solani) such as Rhizoctonia pyricularis (Rollinia solani neri), Rhizoctonia nigripes (Monascus), Rhizoctonia solani such as Monascus, Scleroticus solani (Burkholderia plantago solani), such as Bochybotrytis cinerea (Bochybotrytis cinerea) such as Bochybotrytis cinerea (Bochybotrytis, the genus Venturia nigricans (Venturia) such as Venturia mali (Venturia nasicola), Cochliobolus (Cochliobolus) such as Blastomyces graminis (Cochliobolus miyabeanus), Fusarium (Didymelala) such as Blastomyces cucumerinum (Didymela) and Sclerotinia sclerotiorum such as Sclerotinia brassicae (Sclerotinia sclerotiorum), Candida such as Blastomyces persicae (Monilinia fruticosa), Rhizophora (Rhizophora fragilis) such as Blastomyces persicus (Monilinia fruticosa), Rhizophora such as Rhizophora oryzae (Rhizopus), Phytophora oryzae (Phytophora oryzae) such as Rhizophora solani, Phytophora sp.e, Phytophora solani such as Phytophora sojae (Phytophora solanum), Phytophthora brassica (Phytophora solani) such as Phytophythora solani, Phytophora solani such as Phytophora solani (Phytophora solani), Phytophythora solani such as Phytophytrium solani, Phytophytrium, Such as downy mildew of cucumber (Pseudoperonospora cubensis), fungi of the genus Leptospira (Plasmopara), such as downy mildew of grape (Plasmopara viticola), fungi of the genus Aphanomyces (Aphanomyces), such as Rhizoctonia solani (Aphanomyces cochliae), fungi of the genus Plasmodiophora, such as Rhizobium bovienii (Plasmopara brasiliensis), fungi of the genus Spongospora (Spongospora), such as Rhizoctonia solani (Spongospora subterrata), fungi of the genus Streptomyces, such as Buctomyces solani (Buctomyces sphaera), bacteria of the genus Clavibacter (Clavibacter), such as Buctobacter sphaeroides, bacteria of the genus Clavibacter, such as Buchobacter lycopi (Clavibacter), bacteria of the genus Rabylonifera, such as Rhizoctonia solani (Rhizoctonia), bacteria of the genus Rabyloni, such as Rhizoctonia, Rhizoctonia (Rhizoctonia), bacteria of the genus Rayna, such as Rhizoctonia (Rhizoctonia), such as Rhizoctonia, Rhizoctonia (Rhizoctonia), bacteria of the genus Rayna, such as Rhizoctonia (Rhi, For example, of the genus Novospora oryzae (Acidovorax avenae), of the genus Pseudomonas (Pseudomonas), for example, Bremia lactucae (Pseudomonas spp.), of the genus Xanthomonas (Xanthomonas), for example, Bremia brassicae (Xanthomonas campestris), of the genus mycotic transmissible virus (Furovirus), for example, Zephyra mays (SBWMV), of the genus Tobamovirus, for example, Capsicum microprecisa (PMMoV), of the genus Tobravir (Tobravir), for example, Nicotiana Tabacum (TRV), of the genus Potexvirus, for example, Potexvirus, of the genus Solanum Xvirus (PVX), of Cryptovirus Carlavus (Carlavus), for example, of Caragana latent virus (CaLV), of Rhamnivirus (Crinivirus), for example, Potymovirus retroviruses (CCovyvirus), for example, Pseudovirous graminis (Byvirus), of the genus Alfavus, for example, Povidomyvirus (Tokayavirus (Wymvirus), for example, Povidomyoma melanophora virus (Wyvirus), for example, Povidae, Povid, For example Alfalfa Mosaic Virus (AMV), cucumber mosaic virus (Cucumovirus) genera, such as Cucumber Mosaic Virus (CMV), pea mosaic virus (Comovirus) genera, such as radish mosaic virus (RaMV), leguminous virus (Fabavirus) genera, such as Vicia faba wilt virus (BBWV), nematode-borne polyhedral virus (Nepovirus) genera, such as tomato rotavirus (TomRSV), Citrus unshiu dwarf virus (Sadwavirus) genera, such as Wenzhou atrophy virus (SDV), carnation spot virus (Carmovirus) genera, such as melon wilt spot virus (MNSV), Serpentine virus (Ophiovirus) genera, such as tulip microdystick mosaic virus (TMMMV), Tenuivirus (Tenuirus) genera, such as rice onyx leaf blight virus (RSV), tomato spotted virus (Tosporus) genera, such as yellows virus (TSV), plant chlorotic virus (TSV), such as rice wilt virus (Begoivorv), such as rice wilt virus (RDgoivorv), bean wilt virus (RDV) genera, Such as Tomato Yellow Leaf Curl Virus (TYLCV), cauliflower mosaic virus (Caulimovirus) genus, such as cauliflower mosaic virus (CaMV), potato spindle tuber viroid (Pospiviroid) genus, such as chrysanthemum dwarfing viroid (CSVd), etc., but the present invention is not limited to these examples.

Among the above-mentioned pathogenic bacteria, especially, infectious pathogenic bacteria such as a bacterium belonging to genus Rizophyllum, Rhizoctonia, sclerotinia, Rhizoctonia, anthracnose, Fusarium, Verticillium, Richcardia, Chaetomium, leaf scald disease, Rhizobium, Rhizoctonia, Sclerotinia, Rhizopus, Phytophthora, Saccharomycosis, Rhizomucor, Eleobacter, Streptomyces, Corynebacterium, Soft rot fungus, Rhizobium, Ralstonia, funguses baculovirus, tobacco mosaic virus, tobacco rattlesvirus, barley yellow mosaic virus, nematodidopsis, carnation spot virus, Serpentis can be suitably controlled by the present invention.

Further, as the nematodes that can be controlled by the pesticidal preparation composition of the present invention, specifically, there can be mentioned: the genus of leaf bud nematodes (Aphelenchoides), such as leaf bud nematodes (Aphelenchoides besseyi), the genus of Bursaphelenchus (Bursaphelenchus), such as Bursaphelenchus xylophilus (Bursaphelenchus xylophilus), the genus of stem nematodes (Ditylenchus) such as Heterophyllus putida (Ditylenchus destructor), the genus of Podosphaera solani (Globodera) such as Phlomyces tuberosus (Globodera roseus), the genus of cyst nematodes (Heterodera) such as Phlomyces glycines (Heterodera glycines), the genus of root nodule nematodes (Meloidogyne) such as Meloidogyne incognita (Meloidogyne incognita), the genus of Heterophyllus (Pratoda), such as Brevibacterium punctatus (Penicillium pennychus), such as Meloidogyne incognita (Meloidogyne), the genus of Heterophyllus such as Heterophyllus punctatus (Meloidogyne), but not exemplified by the species of the genus Phyllochulus punctatus, Phyllostachys punctatus (Meloidogyne), and the genus of the invention.

Further, as the pests which can be controlled by the pesticidal preparation composition of the present invention, specifically, there can be mentioned: orthoptera pests, such as Caryoptera nigricans (Ruspolia lineosa) of Caryophoraceae, Rhynchosia lanuginosa (Teleogryllus emma) of Acridaceae, Gryllotalpa orientalis (Gryllotalpa orientalis) of Gryllotalpa, Oryza minutissima (Oxya yezoensis) of Acridaceae, Rhizophora migratoria (Lousta migratoria), Rhizophora migratoria (Melanopus sanguinipes) of Acridaceae, Cyperus siphonaptera (Eugyrus japonicas), Scyphylla siphonata (Xyajaponicaceae), Thysanoptera pests, such as Pseudothrips kawakamii (Frankliniella intensa), Scirpus typhyllum spicata (Frankliniaceae), Scirpus typhus niloticus (Pseudothrips nilotica), Pectinopsis trichoptera (Pseudophilis) of Scirus), Pectinoptera (Pseudothrips nilapaceae, Acridae), Pectinopsis (Pseudothrips caninopsis), Pectinopsis (Pseudothrips nilapa), Pectinopsis) of Thinopsis (Pseudopholidaceae, Pectinopsis), Pectinopsis (Pseudothrips) Grape leafy fruit of the family Cicadae (Arboridia apaticas), tea green leafhopper (Empoasca onaukii), Nephotettix cincticeps (Nephotettix cincticeps), Elephantopis cicadae (Recilia dorsalis), Trapa fuliginosus (Pentataridia apaticas) of the family Fedicellaceae, Laodelphax striatellus (Laodelphax striatellus) of the family Oryzaceae, Nilaparvata lugens (Nigatella furcifera), Bemisia albuginella (Nisia nervosa) of the family Alloideae, Kamendakhara saccharova of the family Oryza, Achillus flamae of the family Orostachys, Oroscynanchus of the family Cicada, Phymatopsis aurantidae (Acacia), Phyllophthora citri nilapaceae (Aphis citrifolia (Califolia), Phyllophora nilapa nilapaceae), Phyllophora niponica (Phyllophora niponica) of the family Cicadataceae, Phyllophora nilapaceae, Phyllophora niphacidae (Phyllochaetaceae), Phyllochaceae (Phyllochaceae), Phyllophora nigella Nilaparvata indica (Physalsa), Phyllochavicola, Phymatocha nikopsorales (Phymatocha (Physalsa), Physalsa) of the family Thiela nikomatochaceae, Phymatochaceae, Phymatocha nikomatochaceae, Phymatocha (Phymatocha nikomatocha nikopsorales (Phymatochai) of the family Thiela), Phymatochaceae), Physalsa), Phymatochai (, Pseudocerus pseudophycus (Lipophis erysimii), Myzus persicae (Myzus persicae), Nepalusthis graminis (Schizaphis graminum), Triales oryzae (Rhopalosiphum padi), Aleurochaeus latus (Aleuroconthus cameral), Trionyx citriodorus (Aleuroconthus spicifera), Trionyx tabaci (Bemisia tabaci), Trionyx argenteus (Bemisia argenteus), Triales viridis (Trialeurodes vaporariorum), Trigonostegia japonica (Drosicha coeruleus) of Lasiosphaera, Trionyx lanugineus (Icaris) of Lasiosphaera, Trigonopsis viridis (Piperidae), Trigonococcus bractes viridis (Euonyx viridis), Trigonococcus viridis (Piperita), Calmetia viridis (Pimenta), Calicina viridis (Euonyx viridis), Calicina viridis (Euonyx canicola (Euonyx viridis), Calicina (Euonyx canicola (Euonyx viridis), Calicina (Euonyx canicola (Euonyx), Calicina viridis (Euonyx canicola (Euonyx canina), Calicina (Calicina), Calicina viridis (Calicina), Cal, Dolichos coronarius (stephanis pyrioides) of the family reticulidae, dolichos piricola (stephanis nashi), stinkbug (eusarcocoris aeneus), stinkbug (lagynomongatus elengatus), lygus lucorum (Nezara viridula), lygus lucorum (plattia grossedentata), lygus guichenoti (megacopharalis) of the family turgidae, lygus hesperus (megacoptida crigaritifera), lygus brevicula (cavel saccharivorvus) of the family longus, stinkbug (dolichopus major) of the family protuberidae, lygus sartorius (dysaricus) of the family wingless, eucarvaceae, eucargus gigas (euonymus fortunei) of the family arachnidae, euonymus fortunei (leucotrichidae), eustoma dorus gigas (leucotrichidae), eustoma plicata (euonymus japonicus), euonymus japonicus (leucotrichidae), euonymus japonicus (euonymus japonicus), euonymus japonicus (euonymus japonicus), eu, Skipjack (Anthrenus verasci) of Katsuwonus Pelamis, Isopteris erythropolis (Heterobotrys hamatopteris) of Kaempferiae, Pitinus chinensis (Stegobium panicum) of Choneminidae, Pitinus clavipes (Carphilus clavipes) of Ascomidae, Trigonopterus magnus (Terebris magni) of Guardiaceae), Heterophylla rubescens (Necrobium rufimicus) of Guardiaceae, Arctium maculatum (Carpophyllum tenella) of Ceratopteridae, Anhalus dorsalis (Ahasveris) of Astrothecaceae, Plactium cera (Cryptospira hominis) (Cryptophylla terreus) of Geraniaceae), Eustia cera (Epyphylla seropteris) of Hippocastaneta (Caryophyllum serotina), Eustigmatis variola terreus (Pseudopteris purpurea) of Californidae, Eupatorium purpurea (Rhynchophylla) of Hypoglaucea), Eupatorium (Rhynchophora purpurea) of Hypocrea), Eupatorium (Rhynchophyllum purpurea) of Myricaceae, Eupatorium), Eupatorium (Rhynchophyllum crocus (Rhynchosia variety (Rhynchosta) of Califolium variety, Eupatori variety of Califolium variety of California), Eupatori variety (Rhynchosia variety of Califolium of Hypoglyphylla variety of Californis), Eupatori variety of California), Eupatori, Corn rootworm (Diabrotica virgifera), yellow melon (autolophora femoralis), cabbage datura (Phaedon brassicae), yellow flea (phyllotretta striata), sweetpotato elephant (Cylas formicarius) of the family tricopitaceae, alfalfa weevil (Hyera potastica) of the family Binodiaceae, vegetable weevil (Listrodensis), sweet potato weevil (Euceps potassalis) of the family Binodiaceae, rice weevil (Echinococcus bipunctatus) of the family Rongkotidae, rice water weevil (Lissophorus oryzae) of the family Binodiaceae, corn weevil (Sitophyllus amas) of the family Binodiaceae, northern weevil (Piperidae), bark beetle (bark beetle) of the family Bingonidae), bark beetle (Piercidae), bark beetle (Pimpinella chinensis), Pimenta beetle (Pimenta), Pimenta beetle (Pimenta) of the family Bignonidae), Pimenta (Pimenta) of the family), Pimenta (Pimenta) of the family), Piper nigrella sinensis (Piper nigrella) of the family, Pimenta (Piper nigra), Pimenta (Pimenta) of the family), Pimenta (Pimenta), Pimenta) of the family), Pimenta (Pimenta) of the family), the plant diseases include soybean pod gall midge (Asphondylia yushimaia) of the family gall midge, wheat gall midge (Mayetiala destructor), Aedes aegypti (Aedes aegypti) of the family Culicidae, Culex pipiens (Culex pipiens pallens), Sapotomycota sinense (Simulium takahashi) of the family Culicidae, Chironomus oryzae (Chironomus oryzae) of the family Chironomus, Tabanus blindanus (Chrysosporium suavis) of the family Tabanidae, Tabanus caninus (Tabanus trigonus) of the family Aphididae, Aphididae griseus (Eumerus strigatatus), Pseudocera cerana japonensis (Euphragma), Merata Meristensis (Meristotheca) of the family Hymenochaetaceae), Mucoraceae, Pimenta cerata indica (Pimenta melanogasta), Pimenta cerata (Pimenta melanogaster) of the family Hymenochaetaceae, Musca fly (Pimenta) of the family, Pimenta melanogaster (Pimenta) of the family, Musca (Pimenta), Pimenta) of the family Hymenochaetaceae, Pimenta (Pimenta) of the family, Pimenta), Pimenta (Pimenta) of the family, Pimenta (Pimenta) of the family, Allium cepa (Delia indica) of the family anthomyzidae, seed flies (Delia platura), Haematola sativa (Fannia canicicularis) of the family Vaticaceae, Sarcophaga carnosa (Musca domestica), Saraca stable (Stomoxys caldarins), Sarcophaga fusca (Sarcophaga peregrina) of the family Sarcophagidae, Gasterilus intestinalis (Gasterphilus intestinalis) of the family Maloideae, Sarcophaga indica (Hypoderma lineulatum) of the family Muscadae, Musca ovis (Oestrus ovis) of the family Muscadae, pests of the order Lepidoptera, such as Glyphyta oblitera (Endocrina excrescens (Endocarpes), Botrytis vitis (Antisia amolopidera) of the family Hypocrea), Botrytis niponica (Hypoglyphi), Botryta niponica (Hypoglyphi punctata (Hypoglyphi), Botryta niponica (Hypoglyphi), Botryta platyphi (Hypoglyphi) of the family Hypoglyphylla, Sphaceae), Botryta platyphylla (Hypoglyphylla cinerea), Botryta (Grapholitura), Sphachis niponaria carassima niponica (Grapholitha californica), Sphaceae), Sphachis niponica (Sphacea, Sphachis niponaria, Sphachis litha niponaria, Sphacea (Sphacea, Sp, Tea leafbud moth (Eumeta minuscula), Plutella xylostella (Nemapogon granella) of the Mylopteraceae family, Chlamydia agnata (Tinea lucens), Plutella xylostella (Bucculatx pyrivorella) of the Onagraceae family, Periplocaceae peach leaf miner (Lyonetia clerkella), Plutella xylostella (Caloptilia theivora) of the Arctiniaceae family, apple leaf miner (Phyllobacter ringer), Phyllostachys citrella (Phyllosticta), Pseudoplutella viridis (Acrolophia saproptera) of the Pseudoplutella family, Plutella xylostella (Plutella xylostella), Plutella xylostella (Hypoglossa niponensis) of the Nedidae family, Plutella xylostella (Phomoptera), Plutella xylostella (Pholidocarpus niponensis), Plutella xylostella (Pholidae), Plutella xylostella punctata (Pholidae), Plutella xylostella punctata (Pholiota), Plutella xylostella punctata (Pholidae), Plutella xylostella), Plutella xylostella salpinoides (Pholidae) of the family, Sphaceae), Plutella punctata (Pholidae), Plutella nikola indica), Plutella niponaria viridae (Plutella frugium, Sphaceae), Plutella xylostella variety (Plutella nipon, Chilo Suppresalis, Cnaphalocrocis medinalis (Cnaphalocrocis medinalis), Asian corn borer (Ostrinia furnacalis), European corn borer (Ostrinia nubilalis), Plutella xylostella (Cadra cautyla) of the family Bombycidae, Cerrena (Galleria mellonella), Bombycis diamondifera (Nippoptilia vitis) of the family Bombycidae, Citrus phoenicis (Papilio xuthusa) of the family Pieris, white butterfly (Pieris rapae) of the family Pieris, single band butterfly (Parara gutata) of the family Pieris, Kidney ulensis (Asphacis) of the family Aphidae, Lyophyllum trichoplusia (Asparagus Setarius) of the family Cochlearia, Lyophyllum trichoplusia (Helicoides) of the family Cyrtymeniaceae, Lyophylla heterospodoptera (Ostrinia heterospodoptera) of the family Helicornia), Plutella xylostella (Ostrinia xylostella) of the family, Lyophylla (Ostrinia armorica) of the family, Sporica (Ostrinia fructica) of the family Helicornidae), Sporica (Ostrinia fructica) of the family Helicornia (Ostrinia terrestria fructica), Sporica) of the family Helicornidae, Spirisfiella (Sporica) of the family Helicosanae, Spirisfiella (Spirisfiella, Spirisfiella (Spirisf, Tobacco bud noctuids (Heliothis virescens), Spodoptera exigua (Spodoptera exigua), prodenia litura (Spodoptera litura), hymenoptera pests, e.g. trichogramma rosea (orange pagana) of the family trichogrammatidae, trichogramma cumingii (ape kuri) of the family phyllopodidae, trichogramma rubrum (athyria rosea ruficum), trichogramma cumingii (dryocosus kuriiurus kuri) of the family trichoderma, rheum officinale (Vespa longipedunculata) of the family vespidae, fire ants (Solenopsis virens) of the family formidaceae, rosa canicola (megachilia nipponica) of the family ceraceae, rhamna pests, e.g. globulus flava (bourita) of the family roundworm (blepharia terrestris), tail pests, e.g. chlamydomonas of the family ichaegaceae, termite (chlamydia), termite pest (septoria) of the family chlamydomonas, september (september), termite (september) of the family neospora, september (september), september (september family september (september), september (september family september (, Termite formosana (Odontottermes formosanus) of the family Bemisiae, pests of the order rodentia, such as the Trionycis sinensis (Trogium pulcherium) of the family Arctidae, rodentia (Liposctelis corrodens) of the family Tinosporaceae, pests of the order Trichophyton, such as the winged lice (Lipeurus caponis) of the family Ornithoideae, the cattle lice (Damalinia bovis) of the family Veitvatidae, pests of the order Anopyrodidae, such as the pig lice (Haematopinus suis) of the family Bemisiae, the body lice (Pediculus humanus) of the family Sirturidae, the dog lice (Linoganathus setosus) of the family Trichophyton, the pubic lice (Pthircus pubis), the parasitic mites of the family Veitleydidae, such as the Triphytes trichophytes (Phytophus chinensis) of the family Microphycidae, the spider mites (Phytophus nilapaceae), the leaf mites (Tetranychus) of the family Tetranychus), Tetranychus (Tetranychus) of the family Tetranychus), and Tetranychus (Tetranychus) of the family Tetranysfungidae), the family Leguminosae), the family, Tetranychus pomi (Panocyrus ulmi), Tetranychus urticae (Tetranychus urticae), Tetranychus meretricus (Tetranychus kazawai), Tetranychus naeslunensis (Triseta pini), Trionyx cantonensis (Aculops pelekassi) of the family Arthropodidae, Ruscus pyricularis (Epitrimerus pyri), Citrus rustus (Phylocotuta oleivora), Rhynchophyllus (Diptacus cristata) of the family Bitylodes luteinidae, Aleuroglyphus ovatus (Aleuroglyphys ovalicatus) of the family Fungidae, Tyrophagus putrescentiae (Tyrophagus putrescentiae), and Rhizopus rubi (Rhizogylphus robinii), but the present invention is not limited to these examples. Among the above pests, soil pests such as thysanoptera pests, coleopteran pests, diptera pests, and lepidoptera pests are particularly preferably controlled by the present invention.

As weeds that can be controlled by the pesticidal preparation composition of the present invention, there can be mentioned: salicaceae weeds, such as Oenothera erythrosepala (Oenothera erythrosepala), Oenothera laciniata, Ranunculaceae weeds, such as Ranunculus japonicus (Ranunculus muricatus), Ranunculus europaea (Ranunculus muricatus), Polygonaceae weeds, such as Polygonum convolvulus (Polygonum convoluulus), Polygonum nigrum (Polygonum lapathiocarpus), Polygonum flaccidum (Polygonum cuspidatum), Polygonum cuspidatum (Polygonum persicum), Polygonum rugosum (Polygonum crinum crassifolia), Polygonum giganteum (Polygonum persicum), Polygonum rugosa (Stelax crispa), Rumex trichocarpa (Polygonum obtusifolia), Polygonum heterophyllum (Poligonuium spicatum), Polygonum cuspidatum (Polygonum syphilum), Polygonum longiligulatum (Steuca), Polygonum fistulosa (Sterculiaceae), Polygonum semiaquilinum (Stephania), Polygonum personatum (Percifera), Polygonum tricolor (Polygonum japonicum), Eucheuma canariella (Polygonum grandiflora), Eucheuma (Califolium), Eucheuma canariella (Califolium), Eucheuma canadensis (Calvata), Eucheuma benaceae weeds (Calvata), Eucheuma (Calvatia), Eucheuma (Calvata), Eucheuma (Calvatica), Eucheuma benaria (Calvatica), Eucheuma (Calvatia (Calvatica), Cal, Chenopodium album (Chenopodium album), Chenopodium album (Chenopodium fistulosum), Amaranthus tricolor (Chenopodium auriculatum), Amaranthus mangostanus), Amaranthus palmeri (Amaranthus retroflexus), Amaranthus mangostanus (Amaranthus hypochondriacus), Amaranthus palmeri (Amaranthus mangus), Amaranthus caudatus (Amaranthus viridis), Amaranthus caudatus (Amaranthus albus), Amaranthus indicus (Amaranthus indicus), Acacia olens (Amaranthus indicus), Capsella sativus (Acacia olens), Acacia olecia sativus (Acacia oleracea), Acacia sativa (L.) and Acacia olens), Monochorium (L.) medicina), Acacia olens (Capsella indica), Acacia sativa (L.) A), Acacia sativa (Thymus), Acacia olecia), Acacia sativa (Acacia), Acacia olecia sativa (L), Acacia sativa (Acacia), Acacia olecia sativa (L (Acacia), Acacia sativa (Acacia), Acacia olecia sativum (Acacia), Acacia sativum (Acacia), Acacia (Acacia sativum (Acacia), Acacia olecia (Acacia sativum (Acacia), Acacia (Acacia ), Acacia (Acacia sativum (Acacia), Acacia olecia), Acacia (Acacia), Acacia (Acacia olecia (Acacia), Acacia olecia), Acacia), wild pea (Vicia sativa), lucerne (Medicago luculina), vetch (Vicia hirsuta), corn (Kummerowia striata), lucerne (Medicago polymorpha), narrow-leaved vetch (Vicia angustifolia), Aeschynomene indica, Malvaceae weeds such as Abutilon Abutilon (Abutilon theopterasi), Sida camara (Sida spinosa), Violaceae weeds such as Viola wild (Viola arvensis), Tri versicolor (Viola tricolor), Symphytum plants such as Galium alatum (Galium aparine), Convolvulaceae weeds such as Ipomoea japonica (Ipomoea), Phaseolus vulgaris (Ipomoea), Lamiopsis sativa (Ipomoea), Lamiopsis thaliana (Ipomoea), Lamiopsis (Ipomoea rosera), Phaseolus sativa (Ipomoea, Lamiopsis (Ipomoea), Lamiopsis (Ipomoea rosea), Lamiopsis (Ipomoea), Lamiopsis (Lamiopsis), Lamiopsis (Lamiopsis), Lamiopsis (Lamiopsis), Lamiopsis thaliana (Lamiopsis), Lamiopsis (Lamiopsis, solanaceae weeds such as Datura stramonium, black nightshade (Solanum nigrum), Physalis angulata (Physalis angustifolia), Solanum phomophilum (Solanum americanum), Solanum rostratum (Solanum nigrum), Solanum rostratum (Solanum carinatum), Scrophulariaceae weeds such as Arabic sodium (Veronica persica), Veronica erecta (Veronica arvensis), Veronica nigra (Veronica nigricana), Asteraceae weeds such as Eclipta prostrata, Chamomilla triphylla (Bidens tripartitata), Xanthium sibiricum (Xanthophyllum sylvestris), Helianthus annuus (Helianthus annuus), Matricaria chamomilla (Matricaria chamomilla), Matricaria chamomilla (Matricaria), Matricaria chamomilla indica (Composita), Matricaria chamomilla (Matricaria chamomilla), Matricaria chamomilla indica (Matricaria), Matricaria chamomilla indica (Matricaria chamomilla), Matricaria chamomilla (Matricaria chamomilla), Matricaria chamomilla) and Matricaria chamomilla (Matricaria chamomilla) of the genus Sisalacia), Matricaria chamomilla, Jerusalem artichoke (Helianthus tuberosus), Silybum marianum (Cirsium arvense), Euphorbia fischeriana (Bidens frondosa), Bidens bipinnata (Bidens pilosa), cornflower (Centurea cyanus), Cirsium vulgare (Cirsium vulgare), Lactuca sativa (Lactuca sativa), Chrysanthemum nigrum (Rudbeckia hirta), Chrysanthemum morifolium (Rudbeckia lacnaria var. hornensis) Var. hordei, Eurycoma paniculata (Senecio vulgaris), Silybum marianum (Silybum marianum), Asclepiadactylum florum (Sonchusana), endive (Sonchus arvensis), Geranium arvensis (Salla), Eupatorium odorata (Caryophyllum arvensis), Eupatorium officinalis (Eupatorium), Eupatorium officinalis (such as, Eupatorium), Eupatorium (Eupatorium) such as, Eupatorium (Eupatorium), oxalidaceae weeds such as Oxalidaceae weeds (Oxalis corrymbosa), Cucurbitaceae weeds such as Citrus lanatus (Sicyos angulus), Gramineae weeds such as Echinochloa japonica (Echinochloa oryzicola), Euphorbia lathyris (Leptochloa chinensis), Indocalamus salicifolius (Isachne globosa), Paspalum distichum (Paspalum distichum), Humicola cutis (Leersia sayanuka), Rong grass (Leersia oryzoides), Echinochloa crusgalli (Echinochloa cruris-galli), Setaria viridis (Setaria viridis), Setaria viridis (Setaria faberi), Digitaria digitata (Digitaria sanguinalis), Eleusinesis indica (Eleusinesis indica), Populicaceae grass (Poa annua), Alternaria sanguinea major (Alnicola), Ostrinia indica (Avenus), Spartina indica (Avenus indica), Spartina indica (Avenus sativa), Spartina indica (Avenus indica), Spartina indica (Avenus sativa), Spartina (Ostreta), Spartina indica (Rotata), Spartina indica (Atrum (Rotata) and Sarmentum (Rotata) including Verbenaria (Rotata) and Sarmentum (Rotata) including (Rotata) and Sarmentum (Rotata) of the genus, Rotata (Rotataricum), Lolium rigidum (Lolium rigidum), Setaria viridis (Setaria glauca), Slicky Slickwood (Beckmannia syzigachy), Commelinaceae weeds such as Commelina communis (Commelina communis), Verruca vulgaris (Murdannia keisak), Equisetaceae weeds such as Equisetum arvense (Equisetum arvense), Papaveraceae weeds such as corn poppy (Papaver rhoeas), Cyperaceae weeds such as Cyperus rotundus (Cyperus irica), Cyperus rotundus (Cyperus rotundus), Cyperus sativus (Cyperus culus), Isodon japonicus (Cyperus difformis), Luciola tenuis (Scirpus flaccidiflora (Scirus), Sargassum bovis seu Bubali viburni (Eleococcus acicularis), Hydrocotyledon aquatica (Cyperus), Cyperus chinensis (Cyperus), Eleococcus neomyces (Scirpus officinalis), Scirpus officinalis (Scirpus chinensis), Scirpus officinalis (Scirpus officinalis), Scirpus officinalis (Scirpus officinalis), Scir, Examples of weeds of the family Oryzaceae include, but are not limited to, Dioscorea americana (Lindernia dubia), Tabanus tabaci (Dopatrinia juncea), Illicium albolanicum (Gratiola japonica), Alternaria angustifolia (Lindernia angustifolia), Alstonia rostrata (Limnophila sessiliflora), Lythraceae weeds such as Arthrospira nodosa (Rotala indica), Amaranthus polygama (Ammannia multiflora), Stellaraceae weeds such as Carum tricornutum (Elatine triandra), Monochoria vaginalis (Monochoria vagianalis), Monochoria glabra (Monochoria korsakosponii), Isotropha lanica (Heteranthus japonicus), Alismataceae weeds such as Arrowia pulata (Sagittaria Sagittaria italica), Sagittifoliata (Sagitalis), Sagitalis indica), Orchidaceae (Ovatia indica), Ovatica (Ovatica), and the like.

The pesticide preparation composition of the present invention can be used to remove a wide range of weeds that are required to control the growth of weeds, such as industrial facilities including slopes of embankments, riverbeds, road shoulders and slopes, railroad footprints, park greens, squares, parking lots, airports, factories, and storage facilities, fallow areas, and vacant areas in cities, or non-agricultural areas where the growth of weeds is to be controlled, such as tree gardens, grasslands, lawns, and forestry areas. In addition, as objects that can be controlled by the pesticidal preparation composition of the present invention, there can be mentioned: rotaphyceae grown in rivers, waterways, canals, reservoirs, etc., such as stonewort (Chara braunii), lemnaceae such as lemna (Spirodela polyriza), moss such as moss (ricicocarpus natrans), diploideae such as Spirogyra arcla, monochamaceae such as nella reticulata (Eichhornia crassipes), sophorae maltaceae such as azonia mangostana (azonia rubra), rhododendron japonicum (Azolla japonica), sophora japonica (salvina natana), astraceae such as water puff (pista), dichondrocaceae such as Myriophyllum pratense (Myriophyllum aquaticum), etc., but the present invention is not limited to these examples.

In the present invention, both the "solid culture of a filamentous fungus as a biopesticide" and the "acetate-adsorbed substance" are not necessarily the products specified by the production method, but the structures are specified only by showing the states. In addition, manipulation or analysis by methods other than those described above to specify the structure of these substances would require significant economic expenditure or time.

The present invention is not limited to the embodiments described above, and various modifications are possible within the technical spirit of the present invention.

Example 1

(test of the effect of acetic acid on the growth of various filamentous fungi spores)

In an inorganic salt medium (0.2 mass% NaNO)₃0.1% by mass of KH₂PO ₄KCl 0.02 mass%, MgSO 0.02 mass%₄・7H₂O) was added with acetic acid at a predetermined concentration, and the resulting mixture was used as a test medium. 0.5ml of spore suspension (1X 10) was added to each of the filamentous fungi of Trichoderma asperellides SKT-1 strain, Trichoderma harzianum T-22 strain and Trichoderma hamatum NBRC 31932 strain⁶Spore/ml) was cultured in a 100-ml Erlenmeyer flask containing 50ml of the test medium under shaking (27 ℃, 120rpm, 3 days). After the culture, the growth of hyphae of each strain was observed. The results of these tests are shown in Table 1.

In addition, the same test was carried out on Talaromyces flavus (flavus) SAY-Y-94-01 strain and Metarhizium anisopliae (anisoplaae) SMZ-2000 strain.

The Trichoderma asperelloides SKT-1 strain is sold under the trade name EcoRope (registered trade name, manufactured by Kumiai chemical industry Co., Ltd.) as a pesticide. Trichoderma harzianum T-22 strain is sold under the trade name Trichoderma (registered trade name, manufactured by Arysta Life Science Co., Ltd.) as a soil improving material. Trichoderma hamatum NBRC 31932 strain was distributed by the independent administrative agency, human product evaluation technology base agency. The strain Brachypodium fulvellum SAY-Y-94-01 is sold under the trade name Tough block (registered trademark, manufactured by Shikkenhei Co., Ltd.) as an agricultural chemical. The strain "Heizia amabilis" SMZ-2000 is sold under the trade name "Pirates granules" (registered trademark, manufactured by Arysta Life Science Co., Ltd.) as a pesticide.

[ Table 1]

The results show that: no hypha growth was observed in the trichoderma spores at an acetic acid concentration of about 0.15 to 0.3 mass%, i.e., even in the case of spores, growth was difficult when acetic acid was simply mixed at a concentration of not less than a certain concentration. Further, the growth of hyphae of spores of Talaromyces flavus SAY-Y-94-01 and of spores of Metarhizium anisopliae SMZ-2000 at each acetic acid concentration was slightly different, but was substantially the same as that of Trichoderma.

Example 2

(storage stability test of pesticide preparation composition)

As the pesticide preparation composition containing acetic acid, 3 kinds of preparations were prepared, i.e., a raw acetic acid-coated preparation and a direct acetic acid injection preparation of the present invention, and a comparative preparation in which 15 vol% of acetic acid was mixed in SKT-1 liquid (trade name: "Ecohope", manufactured by Kumiai chemical industries, Ltd.). The acetic acid crude powder-coated preparation was prepared by adsorbing acetic acid onto white carbon black to give 15 mass% crude powder, and culturing 0.5ml of SKT-1 spore suspension (1X 10) in a 100ml Erlenmeyer flask containing 8g of barley seed (variety: Hayadori 2), 8ml of YG medium (0.3 mass% yeast extract, 2.0 mass% glucose) in a 100ml volume of SKT-1 barley seed medium culture⁶Spores/ml), left to stand at 25 ℃ for 8 days, and then air-dried naturally overnight. Hereinafter, also referred to as SKT-1 strain solid culture): the above original powder = 99: 1 (mass ratio) is fully mixed in a plastic bag for coating. The acetic acid direct injection preparation was prepared by diluting acetic acid in water to form a 15 mass% solution, as a solid culture of SKT-1 strain: the above solution = 99: 1 (mass ratio) the solution was sprayed while mixing the solid culture of SKT-1 strain in a plastic bag by shaking, thereby adsorbing acetic acid. After the preparation, the preparation was packed in an aluminum bag and stored at 5 ℃, and the number of viable bacteria in the preparation was measured by a dilution plate method after 0 day (initial value), 1 day, and 7 days. The results of this test are shown in Table 2.

[ Table 2]

The result is: while the non-acetic acid-coated preparation and the direct acetic acid injection preparation of the filamentous fungus solid culture of the present invention were able to keep filamentous fungi alive to some extent after 7 days of preparation, the comparative product in which acetic acid was simply mixed in the filamentous fungus solution preparation had a smaller number of viable cells immediately after (initial value) than the present invention, and the survival of filamentous fungi was not confirmed after 1 day of preparation.

Example 3

(test of cucumber southern blight controlling Effect of pesticide preparation composition)

After cucumber Sclerotium rolfsii was precultured on a PDA plate medium, the fungus-containing agar sheets were mixed with a soil wheat bran medium (2.5kg sandy loam, 2.0kg wheat bran, 3g yeast extract, 20g glucose, 1L distilled water) and cultured at 27 ℃ for 7 days. The inoculation source and soil (sterilized sandy soil: horticultural soil (trade name: JA Nippi horticultural soil No. 1, manufactured by Nippon Federation Co., Ltd.) = 1: 1 (volume ratio)) were mixed in a ratio of 1: 5 (volume ratio) was mixed well, and the mixture was left in a wet room (27 ℃ C., in the dark) overnight as contaminated soil. Inoculation was carried out by spreading about 15ml of this contaminated soil on the base of plants of cotyledonary stage cucumbers (variety: daily) grown in plastic cups (diameter 6 cm). After inoculation, the acetic acid-uncoated preparation and acetic acid direct injection preparation of example 2 and the SKT-1 strain solid culture were mixed so as to correspond to 500g/m²The amount of the SKT-1 liquid agent is distributed at the base of the cucumber plant, and the SKT-1 liquid agent is prepared by mixing the SKT-1 liquid agent: water = 1: the dispersion diluted at a ratio of 14 (mass ratio) was adjusted to a value of 3L/m²The amount of (A) is measured by pouring into the basal part of a cucumber plant, and the tolclofos-methyl hydrate (trade name: "Rysorex hydrate" (registered trade name), manufactured by Sumitomo chemical Co., Ltd.) is prepared by mixing the following components: water = 1: 999 (mass ratio) of 3L/m²The amount of (c) is poured into the base of the cucumber plant. After the treatment with the drug, the disease was examined for 3 days in a wet room (27 ℃ C., in a dark place), and the control potency was calculated from the following equation. The results of this test are shown in Table 3.

Control titer = (1-diseased plant rate in treated area/diseased plant rate in untreated area) × 100

[ Table 3]

From the results, it is found that: the acetic acid-coated preparation and the acetic acid direct injection preparation of the present invention exhibit a control effect on cucumber southern blight to the same extent as that of tolclofos-methyl hydrate which is an organophosphorus bactericide, and exhibit a control effect higher than that of SKT-1 solid culture or SKT-1 liquid preparation.

Example 4

(test of cucumber southern blight controlling Effect based on the difference in treatment amount of pesticide preparation composition)

A test for controlling cucumber southern blight of the acetic acid raw material-coated preparation was carried out in the same manner as in example 3. The amount of the acetic acid-base-coated preparation dispersed was the same as that in example 3. The results of this test are compared to the data of example 3 and are shown in table 4.

[ Table 4]

The result is: even if the treatment amount of the raw acetic acid-coated preparation of the present invention is increased by the same amount, a sufficient control effect on cucumber southern blight is observed, but improvement of the control effect depending on the treatment amount is not observed.

Example 5

(storage stability, cucumber southern blight control based on the difference in acetic acid blending amount of pesticide preparation composition)

Acetic acid was adsorbed to white carbon black to form 50 mass%, 15 mass%, or 5 mass% raw powder, which was obtained by mixing the SKT-1 strain solid culture: the above original powder = 99: 1 (mass ratio) was thoroughly mixed and coated in a plastic bag, and an acetic acid raw material-coated preparation having an acetic acid content of 0.5 mass%, 0.15 mass%, or 0.05 mass% was prepared. After storage at 4 ℃ for 1 week, the number of viable bacteria in the preparation and the effect of controlling cucumber southern blight were investigated. The number of viable bacteria in the preparation was investigated in the same manner as in example 2, and the cucumber southern blight control effect was investigated in the same manner as in example 3. The test results of the viable count in the preparation are shown in Table 5, and the test results of the cucumber southern blight control effect are shown in Table 6.

[ Table 5]

[ Table 6]

From the results, it is found that: when the amount of acetic acid added is 0.05 to 0.5% by mass, no significant decrease in the number of bacteria during storage is observed, and the cucumber southern blight control effect is comparable to that of tolclofos-methyl hydrate.

Example 6

(cucumber seedling blight (rhizoctonia) control effect of pesticide preparation composition)

Cucumber seedling Rhizoctonia solani AG-4 was pre-cultured in PDA plate medium, and then mixed with agar containing strain in soil wheat bran medium and cultured at 27 deg.C for 7 days. The inoculation source and soil (sterilized sandy loam: horticultural soil = 1: 1 (volume ratio)) were mixed at a ratio of 1: 50 (volume ratio) and left in a wet room (27 ℃ C., dark) overnight as contaminated soil. A corner pot (9 cm square) was filled with 200ml of sterilized river sand and 100ml of contaminated soil in this order. It should be noted that the non-inoculated area is filled with 100ml of soil wheat bran culture medium: soil = 1: 50 (volume ratio) of the mixed mixture was used instead of the contaminated soil. Soil mixing of the acetic acid raw material coating preparation of example 2 (acetic acid blending amount: 0.15%) was carried out by mixing 100ml of contaminated soil and 8.1g of the acetic acid raw material coating preparation (equivalent to 1000 g/m) before filling the contaminated soil in the corner pot²) Mixing in plastic bag, filling into angle pot, irrigating, and sowing cucumber(variety: daily count). The surface layer spreading of the acetic acid raw powder coating agent is carried out by irrigating water, sowing cucumber seeds in the polluted soil, and spreading at a rate of 500g/m²The amount of the fertilizer is uniformly scattered on the surface layer of the soil. For comparison, the seed was seeded with tolclofos-methyl hydrate: water = 1: 499 (mass ratio) dilution equivalent to 3L/m²The spreading liquid is used for soil perfusion treatment. After the treatment with the drug, the mixture was left standing overnight in a wet room (27 ℃ C., in the dark) and then managed in a greenhouse (water was poured on the head). After 8 days of inoculation, an index study was performed according to the following disease index criteria, and the disease incidence, corrected disease incidence and control efficacy were calculated by the following numerical expressions. The results of this test are shown in Table 7.

< disease index >

0: no morbidity;

1: abnormal growth;

2: withered or not germinated.

Incidence = Σ (number of diseased seedlings differing in degree × index)/(number of study seedlings × 2) × 100;

corrected incidence = mean incidence for each region-mean incidence for non-inoculated regions;

control titer = (1-corrected incidence of disease in treated area/corrected incidence of disease in untreated area) × 100.

[ Table 7]

From the results, it is found that: the acetic acid raw powder coating preparation of the invention shows the same control effect as that of a tolclofos-methyl hydration agent on cucumber seedling blight (rhizoctonia solani) in soil mixing treatment and soil surface layer treatment.

Example 7

(cucumber seedling blight (Pythium species) control effect of pesticide preparation composition)

After cucumber seedling rhizoctonia solani (Pythium aphanidermatum) is pre-cultured by a PDA plate culture medium, fungus-containing agar sheets are mixed in a soil wheat bran culture medium and cultured for 7 days at the temperature of 27 ℃. The inoculation source andsoil (sterilized sandy loam: horticultural soil = 1: 1 (volume ratio)) was mixed at a ratio of 1: 300 (volume ratio) was mixed well, and the mixture was left in a wet room (27 ℃ C., dark place) overnight as contaminated soil. A corner pot (9 cm square) was filled with 200ml of sterilized river sand and 100ml of contaminated soil in this order. It should be noted that the non-inoculated area is filled with 100ml of soil wheat bran culture medium: soil = 1: 300 (volume ratio) of the mixed mixture to replace the contaminated soil. Soil mixing of the acetic acid raw material-coating preparation of example 2 (acetic acid blending amount: 0.15 mass%) was carried out by mixing 100ml of contaminated soil and 4.1g of the acetic acid raw material-coating preparation (equivalent to 500 g/m) before filling the contaminated soil in the corner pot²) Mixed in a plastic bag, filled in a corner pot, subjected to watering, and then sown with cucumbers (variety: daily). After sowing, the seed was treated with an indigenous disinfectant (hydroxyisoxazole liquid) (trade name: "Tachigaren liquid" (registered trade name), manufactured by Mitsui chemical Agro corporation) using: water = 1: 499 (volume ratio) dilution equivalent to 3L/m²The spreading liquid is used for soil perfusion treatment. After the treatment with the agent, the mixture was allowed to stand overnight in a wet room (27 ℃ C., in a dark place) and then was managed in a greenhouse. 12 days after inoculation, an index study was performed according to the following disease index criteria, and the disease incidence, corrected disease incidence and control efficacy were calculated by the following numerical expressions. The results of this test are shown in Table 8.

< disease index >

0: no morbidity;

1: abnormal growth;

2: withered or not germinated.

[ Table 8]

From the results, it is found that: the control effect on cucumber seedling blight (pythium aphanidermatum) to the same degree as that of a soil bacterium disinfectant is shown by carrying out soil mixing treatment on the acetic acid raw powder coating preparation of the invention.

Example 8

(cucumber southern blight control effect based on differences in various grain media of pesticide preparation composition)

As the grain to be tested, rice seed (variety: Kinuhikari), wheat seed (variety: agriculture and forestry No. 61), barley seed (variety: Hayadori 2), soybean seed (variety: Enrei), rice husk, and wheat bran were used. 0.5ml of spore suspension of SKT-1 strain (1X 10) was inoculated to a 100ml Erlenmeyer flask containing 8g of each grain and 8ml of YG medium (0.3% by mass of yeast extract and 2.0% by mass of glucose)⁶Spores/ml), and incubated at 25 ℃ for 8 days. After the culture, the SKT-1 strain culture and a raw material containing acetic acid adsorbed to white carbon black in an amount of 15 mass% were air-dried overnight at 99: 1 (mass ratio) was thoroughly mixed and coated in a plastic bag to prepare an acetic acid raw powder-coated preparation. For these preparations, cucumber southern blight control effects were investigated by the same method as in example 3. The results of this test are shown in Table 9.

[ Table 9]

From the results, it is found that: a preparation containing a solid culture of SKT-1 strain obtained by using various grains as a solid culture medium and acetic acid shows a control effect on cucumber southern blight to the same extent as or higher than that of tolclofos-methyl hydrate.

The present invention is summarized as follows.

The object of the present invention is to provide: a microbial pesticide preparation composition which can be stored for a long period of time in a microbial pesticide preparation composition in which acetic acid is highly activated as a biopesticide filamentous bacterium which is a pesticide active ingredient having a controlling effect on plant diseases and insect pests and/or weeds and a plant growth regulating effect; a process for the preparation thereof; and a method of controlling pests or weeds based on the use of the composition; plant growth regulation methods, and the like.

The above object is also achieved by using an agricultural chemical preparation composition comprising a solid culture of filamentous biological agricultural chemicals as a solid medium containing at least 1 kind of seeds selected from grains, husks, bran and ground materials thereof and acetic acid.

Deposit number

The deposit numbers of the microorganisms deposited internationally in the present invention are shown below.

(1) Trichoderma asperelloides SKT-1 strain (FERM BP-16510).

。

Claims

1. A pesticidal preparation composition comprising a solid culture of filamentous fungi as a biopesticide as a solid medium comprising at least one member selected from the group consisting of seeds of grain, husks, bran and ground material thereof and acetic acid, wherein the activity of the filamentous fungi as a pesticidal component is enhanced by promoting the growth of hyphae by the acetic acid, and the pesticidal preparation composition is a single pesticidal preparation composition as an industrial product to be preserved,

the biological pesticide filamentous fungi is Trichoderma asperelloides strain with effects of preventing and treating plant diseases and insect pests and/or weeds and regulating plant growth,

the content of acetic acid in the pesticide preparation composition is 0.01-1% by mass.

2. The pesticidal preparation composition according to claim 1, wherein the species of asperelloides is Trichoderma asperelloides SKT-1 strain (FERM BP-16510).

3. The pesticidal formulation composition of claim 1, wherein: 10-10 g of pesticide preparation composition¹⁰A filamentous fungus as a biopesticide of CFU which is a colony forming unitColony Forming Unit。

4. The pesticidal formulation composition of claim 1, wherein the solid medium is a millbase of a cereal seed.

5. The agricultural chemical preparation composition according to claim 1, wherein the cereal is a cereal of the family Poaceae.

6. The pesticidal composition according to claim 5, wherein the gramineous grain is barley.

7. The pesticidal formulation composition of any one of claims 1 to 6, wherein: the pesticide preparation composition is a granular pesticide preparation composition, and the solid culture medium is a granular substance.

8. The agrochemical composition according to claim 7, wherein the acetic acid is an acetic acid-adsorbed substance formed by adsorbing the acetic acid to the fine mineral powder.

9. The agricultural chemical preparation composition according to claim 8, wherein the fine mineral powder is hydrous amorphous silica.

10. The agrochemical composition according to claim 8, wherein the content of acetic acid-adsorbed material in the agrochemical composition is 0.5 to 2% by mass.

11. The preparation method of the pesticide preparation composition is characterized by comprising the following steps: a method for producing a pesticidal composition comprising the steps of subjecting a filamentous biopesticide to solid culture using at least one member selected from the group consisting of seeds of grain, husks, bran and ground matter thereof as a solid medium to obtain a solid culture of a filamentous biopesticide with the cultured filamentous biopesticide adhered to the solid medium, adding acetic acid to the culture, promoting the growth of mycelia of the filamentous biopesticide with the acetic acid to increase the activity of the filamentous fungi as a pesticidal component and enable the filamentous fungi to survive for a long period of time, and packaging the filamentous fungi in a single package as a predetermined preserved industrial product,

the biological pesticide filamentous fungi is at least one of asperellides strain of Trichoderma with effects of preventing and treating plant diseases and insect pests and/or weeds and regulating plant growth,

12. The method of claim 11, wherein: the pesticide preparation composition is a granular substance, and the solid culture medium is a granular substance.

13. The method of claim 11 or 12, wherein: acetic acid is carried by spraying acetic acid or a solution obtained by diluting acetic acid with water or an organic solvent onto the surface of a solid culture of a biopesticide filamentous bacterium.

14. The method of claim 12, wherein: acetic acid is adsorbed to the mineral fine powder, and the adsorbate is coated on the surface of the solid culture of the biopesticide filamentous bacteria.

15. The method of claim 14, wherein the fine mineral powder is hydrous amorphous silica.

16. A method for controlling at least one selected from weeds, diseases and pests and/or regulating plant growth, characterized in that: applying the pesticidal formulation composition of any one of claims 1 to 10 to a seedling, a seedling or a seed of a plant, and cultivating the plant.

17. A method for controlling at least one selected from weeds, diseases and pests and/or regulating plant growth, characterized in that: applying the pesticidal formulation composition according to any one of claims 1 to 10 to soil in which plants are cultivated.

18. The method of claim 17, wherein: every 1m²5-1000 g of pesticide preparation composition applied to soilA compound (I) is provided.