CN109207363A - Laminar-flow type living cells culture device - Google Patents
Laminar-flow type living cells culture device Download PDFInfo
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- CN109207363A CN109207363A CN201811097205.0A CN201811097205A CN109207363A CN 109207363 A CN109207363 A CN 109207363A CN 201811097205 A CN201811097205 A CN 201811097205A CN 109207363 A CN109207363 A CN 109207363A
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- layer
- culture dish
- filter layer
- blower fan
- snorkel
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/02—Form or structure of the vessel
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/02—Form or structure of the vessel
- C12M23/10—Petri dish
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M29/00—Means for introduction, extraction or recirculation of materials, e.g. pumps
- C12M29/04—Filters; Permeable or porous membranes or plates, e.g. dialysis
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M29/00—Means for introduction, extraction or recirculation of materials, e.g. pumps
- C12M29/06—Nozzles; Sprayers; Spargers; Diffusers
- C12M29/08—Air lift
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
- C12M41/12—Means for regulation, monitoring, measurement or control, e.g. flow regulation of temperature
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- Health & Medical Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Wood Science & Technology (AREA)
- Organic Chemistry (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Zoology (AREA)
- Biomedical Technology (AREA)
- Sustainable Development (AREA)
- Microbiology (AREA)
- Biotechnology (AREA)
- Biochemistry (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Clinical Laboratory Science (AREA)
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- Apparatus Associated With Microorganisms And Enzymes (AREA)
Abstract
The present invention discloses a kind of laminar-flow type living cells culture device, including incubator, first filter layer, second filter layer, two-way blower fan, total pipeline, upper layer culture dish, temperature controlled compartment, middle layer culture dish, lower layer's culture dish, the upper layer of incubator is equipped with the first filter layer, first filter layer has the second filter layer, second filter layer has two-way blower fan, the exit of two-way blower fan is equipped with total pipeline, total pipeline is connected to snorkel by branch pipe, upper layer culture dish is successively arranged under total pipeline, middle layer culture dish, lower layer's culture dish, upper layer culture dish, middle layer culture dish, snorkel is equipped between lower layer's culture dish, and temperature controlled compartment is additionally provided between upper layer culture dish and snorkel；The invention can ensure that the cleaning of air, avoids the cell of culture from being influenced by external environment, two-way blower fan can adjust the humidity and temperature inside culture dish as needed, different types of cell is cultivated simultaneously, incubator generally ampuliform, facilitate airflow filtering with it is unobstructed, constitute excellent culture environment.
Description
Technical field
The present invention relates to a kind of biological cloning field devices, especially a kind of laminar-flow type living cells culture for improving survival rate
Equipment.
Background technique
The growth of cell needs nutrient environment, and the nutrient matrix for maintaining cell to grow is known as culture medium.Culture medium is pressed
Its physical state can be divided into fluid nutrient medium and solid medium.Fluid nutrient medium is used for large-scale industrial production and physiology
The research work of the basic theories such as metabolism.Certain coagulator (such as agar) or solid culture are added in fluid nutrient medium (such as
Wheat bran, rice etc.) it becomes as solid medium.Solid medium provides the table of a nutrition and ventilation for the growth of cell
Face, the cell produced on such a nutrition surface can form single bacterium colony.
Therefore, solid medium the separation of cell, identification, in terms of play considerable effect.From mostly carefully
The cell and engineered ex vivo, observation are carried out to cell that cell required for separating in born of the same parents' biology and amplification obtain, it is necessary to solve thin
Born of the same parents' in vitro culture problem, compared with the difficulty or ease of microbial cell culture, relatively difficult is from the unicellular of multicellular organism
Culture, the especially culture of zooblast.Cell culture refers to all in vitro cultures, and meaning refers to be taken in driven object living body body
Tissue out carries out incubation culture, is allowed to survive and grow under the conditions of physiological environment in analogue body etc. is specific in vivo.Cell
Training has been widely used in the every field such as biology, medicine, new drug development, become most important basic science it
One.
Summary of the invention
The technical solution adopted by the present invention to solve the technical problems is: the present invention includes incubator, the first filter layer,
Two filter layers, two-way blower fan, total pipeline, upper layer culture dish, temperature controlled compartment, middle layer culture dish, lower layer's culture dish, snorkel, branch pipe,
The upper layer of incubator is equipped with the first filter layer, and the first filter layer has the second filter layer, and the second filter layer has double
To blower, the exit of two-way blower fan is equipped with total pipeline, and total pipeline is connected to snorkel by branch pipe, is successively arranged under total pipeline
Layer culture dish, middle layer culture dish, lower layer's culture dish, upper layer culture dish, middle layer culture dish, be equipped between lower layer's culture dish it is logical
Tracheae, and temperature controlled compartment is additionally provided between upper layer culture dish and snorkel.
Further, the incubator generally ampuliform, bottle mouth position is equipped with the first filter layer and the second filter layer, bottleneck are set
There is two-way blower fan, is equipped with upper layer culture dish, middle layer culture dish, lower layer's culture dish and snorkel in body.
Further, the material of the incubator is organic glass.
Further, the two-way blower fan specifically includes the exhaust fan and air blower being set side by side.
Further, first filter layer is specially honeycomb filter plate, and second filter layer is specially silica wool layer.
Further, liquid is equipped in the temperature controlled compartment to rise formula temperature controller.
The invention has the advantages that filter layer can guarantee the cleaning of air, avoid the cell of culture by external environment
It influences, two-way blower fan can adjust the humidity and temperature inside culture dish as needed, and culture dish is divided into three layers of upper, middle and lower, in addition may be used
Cultivate different types of cell simultaneously, incubator generally ampuliform, facilitate the filtering of air-flow with it is unobstructed, constitute excellent culture ring
Border.
Detailed description of the invention
Fig. 1 is the structural schematic diagram of the embodiment of the present invention.
In figure: 1, incubator；2, the first filter layer；3, the second filter layer；4, two-way blower fan；5, total pipeline；6, upper layer
Culture dish；7, temperature controlled compartment；8, middle layer culture dish；9, lower layer's culture dish；10, snorkel；11 branch pipes.
Specific embodiment
In embodiment, the present invention includes incubator 1, the first filter layer 2, the second filter layer 3, two-way blower fan 4, total pipeline
5, upper layer culture dish 6, temperature controlled compartment 7, middle layer culture dish 8, lower layer's culture dish 9, snorkel 10, branch pipe 11.The upper layer of incubator 1 is set
There is the first filter layer 2, the first filter layer 2 has the second filter layer 3, and the second filter layer 3 has two-way blower fan 4, two-way wind
The exit of machine 4 is equipped with total pipeline 5, and total pipeline 5 is connected to snorkel 10 by branch pipe 11, and upper layer training is successively arranged under total pipeline 5
Ware 6, middle layer culture dish 8, lower layer's culture dish 9 are supported, upper layer culture dish 6, middle layer culture dish 8 are equipped between lower layer's culture dish 9
Snorkel 10, and temperature controlled compartment 7 is additionally provided between upper layer culture dish 6 and snorkel 10；The incubator 1 generally ampuliform, bottle
Mouthful place is equipped with the first filter layer 2 and the second filter layer 3, and bottleneck is equipped with two-way blower fan 4, in body equipped with upper layer culture dish 6, in
Layer culture dish 8, lower layer's culture dish 9 and snorkel 10；The material of the incubator 1 is organic glass；The two-way blower fan 4 has
Body includes the exhaust fan and air blower being set side by side；First filter layer 2 is specially honeycomb filter plate, second filtering
Layer 3 is specially silica wool layer；Liquid is equipped in the temperature controlled compartment 7 to rise formula temperature controller.
After the laminar-flow type living cells culture device is installed by normal procedure, experimenter can be by different types of living cells
It is respectively placed in upper layer culture dish 6, middle layer culture dish 8, lower layer's culture dish 9, the material of the incubator 1 is organic glass, side
Just observe, the generally ampuliform of incubator 1, facilitate the filtering of air-flow with it is unobstructed, constitute excellent culture environment and facilitate taking-up, temperature control
Room 7 and snorkel 10 can guarantee the temperature and humidity inside culture device.
As known by the technical knowledge, the present invention can pass through the embodiment party of other essence without departing from its spirit or essential feature
Case is realized.Embodiment disclosed above, in all respects are merely illustrative, not the only.It is all this
It is included in the invention in invention scope or in the change being equal in the scope of the present invention.
Claims (6)
1. a kind of laminar-flow type living cells culture device, including incubator, the first filter layer, the second filter layer, two-way blower fan, general pipeline
Road, upper layer culture dish, temperature controlled compartment, middle layer culture dish, lower layer's culture dish, snorkel, branch pipe, which is characterized in that incubator it is upper
Layer is equipped with the first filter layer, and the first filter layer has the second filter layer, and the second filter layer has two-way blower fan, two-way blower fan
Exit be equipped with total pipeline, total pipeline is connected to snorkel by branch pipe, upper layer culture dish is successively arranged under total pipeline, middle layer is trained
Ware, lower layer's culture dish are supported, upper layer culture dish, middle layer culture dish are equipped with snorkel between lower layer's culture dish, and upper layer is trained
It supports and is additionally provided with temperature controlled compartment between ware and snorkel.
2. a kind of laminar-flow type living cells culture device according to claim 1, which is characterized in that the incubator is generally
Ampuliform, bottle mouth position are equipped with the first filter layer and the second filter layer, and bottleneck is equipped with two-way blower fan, is equipped with upper layer in body and cultivates
Ware, middle layer culture dish, lower layer's culture dish and snorkel.
3. a kind of laminar-flow type living cells culture device according to claim 1, which is characterized in that the material of the incubator
For organic glass.
4. a kind of laminar-flow type living cells culture device according to claim 1, which is characterized in that the two-way blower fan is specific
Including the exhaust fan and air blower being set side by side.
5. a kind of laminar-flow type living cells culture device according to claim 1, which is characterized in that the first filter layer tool
Body is honeycomb filter plate, and second filter layer is specially silica wool layer.
6. a kind of laminar-flow type living cells culture device according to claim 1, which is characterized in that be equipped in the temperature controlled compartment
Liquid rises formula temperature controller.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201811097205.0A CN109207363A (en) | 2018-09-20 | 2018-09-20 | Laminar-flow type living cells culture device |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201811097205.0A CN109207363A (en) | 2018-09-20 | 2018-09-20 | Laminar-flow type living cells culture device |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN109207363A true CN109207363A (en) | 2019-01-15 |
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Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201811097205.0A Withdrawn CN109207363A (en) | 2018-09-20 | 2018-09-20 | Laminar-flow type living cells culture device |
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| CN (1) | CN109207363A (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110804550A (en) * | 2019-11-25 | 2020-02-18 | 安徽科门生物科技有限公司 | Cell co-culture orifice plate box |
Citations (5)
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|---|---|---|---|---|
| CN102099459A (en) * | 2008-07-16 | 2011-06-15 | 米利波尔公司 | A single or multitier cell culture system |
| US20120315693A1 (en) * | 2005-07-26 | 2012-12-13 | Martin Gregory R | Multilayered cell culture apparatus |
| CN206219596U (en) * | 2016-11-04 | 2017-06-06 | 牟文凤 | A kind of laminar-flow type living cells culture device |
| CN107245441A (en) * | 2017-06-29 | 2017-10-13 | 天津大学 | A kind of microalgae culture system with ventilatory function and multi-faceted adjustable illumination |
| CN206843465U (en) * | 2017-04-16 | 2018-01-05 | 延安大学 | A kind of molecular biology culture dish |
-
2018
- 2018-09-20 CN CN201811097205.0A patent/CN109207363A/en not_active Withdrawn
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20120315693A1 (en) * | 2005-07-26 | 2012-12-13 | Martin Gregory R | Multilayered cell culture apparatus |
| CN102099459A (en) * | 2008-07-16 | 2011-06-15 | 米利波尔公司 | A single or multitier cell culture system |
| CN206219596U (en) * | 2016-11-04 | 2017-06-06 | 牟文凤 | A kind of laminar-flow type living cells culture device |
| CN206843465U (en) * | 2017-04-16 | 2018-01-05 | 延安大学 | A kind of molecular biology culture dish |
| CN107245441A (en) * | 2017-06-29 | 2017-10-13 | 天津大学 | A kind of microalgae culture system with ventilatory function and multi-faceted adjustable illumination |
Non-Patent Citations (2)
| Title |
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| CHIEN-CHUNG PENG ET AL.: "A microfluidic cell culture array with various oxygen tensions", 《LAB CHIP》 * |
| 康宁公司: "康宁HYPERFlask多层细胞培养瓶大规模培养甲型流感病毒", 《中国生物制品学杂志》 * |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110804550A (en) * | 2019-11-25 | 2020-02-18 | 安徽科门生物科技有限公司 | Cell co-culture orifice plate box |
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| WW01 | Invention patent application withdrawn after publication |
Application publication date: 20190115 |
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| WW01 | Invention patent application withdrawn after publication |