CN104862226A - Microbial incubator - Google Patents
Microbial incubator Download PDFInfo
- Publication number
- CN104862226A CN104862226A CN201410058742.XA CN201410058742A CN104862226A CN 104862226 A CN104862226 A CN 104862226A CN 201410058742 A CN201410058742 A CN 201410058742A CN 104862226 A CN104862226 A CN 104862226A
- Authority
- CN
- China
- Prior art keywords
- culture
- videomicroscopy
- mobile platform
- unit
- cultivation
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Landscapes
- Apparatus Associated With Microorganisms And Enzymes (AREA)
Abstract
The invention belongs to the technical field of bio-culture devices, and specifically discloses a microbial incubator, which comprises a box and a control system arranged on the box, and further comprises a plurality of mutually independent culture units, a video microscope and a XYZ moving platform, wherein the culture units are arranged on the inner-upper portion of the box, the video microscope is arranged on the XYZ moving platform, the XYZ moving platform is arranged on the inner-lower portion of the box, the outer wall of the culture unit is prepared by using a transparent material, and the control system is respectively connected with the video microscope and the XYZ moving platform. According to the present invention, the plurality of the mutually independent culture units are arranged so as to provide the independent space for cell culture, the video microscope can move to any culture unit through the XYZ moving platform so as to real-timely observe the growth and development condition of the cells in the culture unit, and with the microbial incubator of the present invention, the culture conditions of a variety of different cells to be tested can be simultaneously and real-timely observed.
Description
[technical field]
The invention belongs to organism culturing device technical field, particularly a kind of microscopic biological incubator.
[background technology]
Associating embryo transfer technology (IVF) in vitro fertilization, also known as tube baby, refers to and allows ovum and sperm be fertilized in vitro by artificial means and carry out early embryonic development, is then transplanted in maternal uterine and grows and the baby of birth.Zygote is cultivated in vitro and within 48 ~ 72 hours, can be grown to 8 ~ 16 cell stage embryos.
The conditional request that zygote is cultivated in vitro is very strict, needs to use special cell culture incubator.And be the developmental state of better observing zygote, need micro-and equipment that is imaging and Real Time Observation is carried out to zygote.Consider the importance of zygote to patient, the zygote of often couple of patient is preferably placed on a space independently isolated with the zygote of other patients and cultivates, in case make mistakes.
At present, existing viable cell incubator system is sold on the market.The CO of OKO-lab company of Italy
2microscope cage incubator, its whole microscope drape gets up to control its environment, is used for maintaining the external environment needed for the cultivation of microscope peripheral cell, by control temperature, humidity, CO
2and the condition such as air pressure ensures cell normal growth state, to complete the viable cell workstation of viable cell long-term observation test; But the zygote that this incubator can not realize each patient has the requirement of an independently culture space, and microscope can only observe the development of fertilized ova situation of a patient.
The desk-top three gas incubator G185 of K-Systems company of Denmark have multiple small-sized independent culturing room, and each culturing room independently can carry out IVF cultivation, but the function that this incubator does not have microscope real time imagery to observe.
Patent 201120052428.2 discloses a kind of real-time recording system for box-type living cell culture, its box incubator adopted uses multiple microscope simultaneously to the cell imaging simultaneously of the multiple positions on culture plate, but can not realize the requirement that the embryonic cell development condition of multiple individual patient observed by a microscope simultaneously.
[summary of the invention]
Goal of the invention of the present invention is to provide a kind of microscopic biological incubator.
Goal of the invention of the present invention is achieved through the following technical solutions: a kind of microscopic biological incubator, comprises casing and is arranged at the Controlling System on casing; Described microscopic biological incubator also comprises multiple separate cultivation unit, videomicroscopy and XYZ mobile platform, and described cultivation unit is arranged at the interior top of casing; Described videomicroscopy is arranged on XYZ mobile platform, and described XYZ mobile platform is arranged at the interior below of casing; The outer wall of described cultivation unit adopts transparent material preparation.
Described Controlling System is connected with videomicroscopy, XYZ mobile platform respectively; Controlling System is mainly used in controlling videomicroscopy and XYZ mobile platform, makes observer better can observe the growing state cultivating Object of Development inside unit.
Described XYZ mobile platform can movement arbitrarily.
Described transparent material is preferably sheet of transparent material;
Described transparent material is preferably glass, PMMA or PVC.
Described microscopic biological incubator can be applied to the cultivation of cell.
Described microscopic biological incubator is preferably applied to the cultivation of embryonic cell.
The interior top of microscopic biological incubator can arrange multiple separate cultivation unit, and each cultivation unit all can place multiple culture dish, can provide independently space for the zygote of often couple of patient, carries out independently IVF and cultivates.The outer wall of each cultivation unit adopts transparent material preparation.Videomicroscopy moves to any one by XYZ mobile platform and cultivates unit, through the transparent material cultivating unit outer wall, and can the growth and development state of embryo on Real Time Observation culture dish.Each space of independently cultivating unit and videomicroscopy is seal isolation, and the conditions such as the humidity in culturing room and steam can not have influence on the normal work of videomicroscopy.
The present invention has following advantage and beneficial effect relative to prior art: the present invention arranges multiple separate cultivation unit above in microscopic biological incubator, can for treating that culturing cell provides independently space.Videomicroscopy moves to any one by XYZ mobile platform and cultivates unit, through the transparent material cultivating unit outer wall, and can the growth and development state of embryo on Real Time Observation culture dish.Each space of cultivating unit and videomicroscopy is seal isolation, and the conditions such as the humidity in cultivation unit and steam can not have influence on the normal work of videomicroscopy.By microscopic biological incubator of the present invention, can the cultivation situation of the simultaneously multiple different cell to be measured of Real Time Observation.
[accompanying drawing explanation]
Fig. 1 is the structural representation of the microscopic biological incubator of embodiment 1;
Fig. 2 is the internal structure schematic diagram of the microscopic biological incubator of embodiment 1;
Wherein: 1-cultivates unit, 2-XYZ mobile platform, 3-videomicroscopy.
[embodiment]
Below in conjunction with embodiment and accompanying drawing, the present invention is described in further detail, but protection scope of the present invention is not limited to this.
Embodiment 1
A kind of microscopic biological incubator, comprises casing and is arranged at the Controlling System on casing; Described microscopic biological incubator also comprises multiple separate, that size is identical cultivation unit 1, videomicroscopy 3 and XYZ mobile platform 2, cultivates the interior top that unit 1 is arranged at casing; Videomicroscopy 3 is arranged on XYZ mobile platform 2, and XYZ mobile platform 2 is arranged at the interior below of casing; The outer wall cultivating unit 1 adopts glass preparation that is transparent, sheet;
Controlling System is connected with videomicroscopy, XYZ mobile platform respectively; Controlling System is mainly used in controlling videomicroscopy and XYZ mobile platform, makes observer better can observe the growing state cultivating Object of Development inside unit.
XYZ mobile platform 2 can movement arbitrarily.
This microscopic biological incubator can be applied to the cultivation of cell, is particularly suitable for the cultivation of embryonic cell.
The interior top of microscopic biological incubator can arrange multiple separate cultivation unit, and each cultivation unit all can place multiple culture dish, can provide independently space for the zygote of often couple of patient, carries out independently IVF and cultivates.The outer wall of each cultivation unit adopts transparent material preparation.Videomicroscopy moves to any one by XYZ mobile platform and cultivates unit, through the transparent material cultivating unit outer wall, and can the growth and development state of embryo on Real Time Observation culture dish.Each space of independently cultivating unit and videomicroscopy is seal isolation, and the conditions such as the humidity in cultivation unit and steam can not have influence on the normal work of videomicroscopy.
Embodiment 2
A kind of microscopic biological incubator, comprises casing and is arranged at the Controlling System on casing; Described microscopic biological incubator also comprises multiple separate, that vary in size cultivation unit 1, videomicroscopy 3 and XYZ mobile platform 2, cultivates the interior top that unit 1 is arranged at casing; Videomicroscopy 3 is arranged on XYZ mobile platform 2, and XYZ mobile platform 2 is arranged at the interior below of casing; The outer wall cultivating unit 1 adopts PMMA preparation that is transparent, sheet;
Controlling System is connected with videomicroscopy, XYZ mobile platform respectively; Controlling System is mainly used in controlling videomicroscopy and XYZ mobile platform, makes observer better can observe the growing state cultivating Object of Development inside unit.
XYZ mobile platform 2 can movement arbitrarily.
This microscopic biological incubator can be applied to the cultivation of cell, is particularly suitable for the cultivation of embryonic cell.
The interior top of microscopic biological incubator can arrange multiple separate cultivation unit, and each cultivation unit all can place multiple culture dish, can provide independently space for the zygote of often couple of patient, carries out independently IVF and cultivates.The outer wall of each cultivation unit adopts transparent material preparation.Videomicroscopy moves to any one by XYZ mobile platform and cultivates unit, through the transparent material cultivating unit outer wall, and can the growth and development state of embryo on Real Time Observation culture dish.Each space of independently cultivating unit and videomicroscopy is seal isolation, and the conditions such as the humidity in cultivation unit and steam can not have influence on the normal work of videomicroscopy.
The above the specific embodiment of the present invention, does not form limiting the scope of the present invention.Any various other done by technical conceive of the present invention change and distortion accordingly, all should be included in the protection domain of the claims in the present invention.
Claims (6)
1. a microscopic biological incubator, comprise casing and be arranged at the Controlling System on casing, it is characterized in that, also comprise multiple separate cultivation unit, videomicroscopy and XYZ mobile platform, described cultivation unit is arranged at the interior top of casing; Described videomicroscopy is arranged on XYZ mobile platform, and described XYZ mobile platform is arranged at the interior below of casing; The outer wall of described cultivation unit adopts transparent material preparation.
2. microscopic biological incubator according to claim 1, is characterized in that, described Controlling System is connected with videomicroscopy, XYZ mobile platform respectively.
3. microscopic biological incubator according to claim 1, is characterized in that, described transparent material is sheet of transparent material.
4. the microscopic biological incubator according to claim 1 or 3, is characterized in that, described transparent material is glass, PMMA or PVC.
5. the microscopic biological incubator described in claim 1 or 2 is applied to the cultivation of cell.
6. the application of microscopic biological incubator according to claim 5, it is characterized in that, described microscopic biological incubator is applied to the cultivation of embryonic cell.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201410058742.XA CN104862226A (en) | 2014-02-20 | 2014-02-20 | Microbial incubator |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201410058742.XA CN104862226A (en) | 2014-02-20 | 2014-02-20 | Microbial incubator |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN104862226A true CN104862226A (en) | 2015-08-26 |
Family
ID=53908408
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201410058742.XA Pending CN104862226A (en) | 2014-02-20 | 2014-02-20 | Microbial incubator |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN104862226A (en) |
Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106399048A (en) * | 2016-09-25 | 2017-02-15 | 东莞市联洲知识产权运营管理有限公司 | Improved microbiological incubator |
| CN106399049A (en) * | 2016-09-25 | 2017-02-15 | 东莞市联洲知识产权运营管理有限公司 | Lightproof microbiological incubator |
| CN106479878A (en) * | 2016-09-25 | 2017-03-08 | 东莞市联洲知识产权运营管理有限公司 | A kind of with removable micro- aobvious bio-incubator |
| CN110747160A (en) * | 2019-11-27 | 2020-02-04 | 浙江大学 | High-survival-rate sheep fertilized egg culture method for extra-embryonic-body culture |
| CN111534480A (en) * | 2019-11-01 | 2020-08-14 | 浙江大学 | In-vitro culture method of mammalian embryo |
| CN112384606A (en) * | 2018-07-06 | 2021-02-19 | 北海道公立大学法人札幌医科大学 | Observation device |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP4652801B2 (en) * | 2004-12-22 | 2011-03-16 | オリンパス株式会社 | Transmission illumination apparatus, microscope equipped with the same, and transmission illumination method |
| US20110091964A1 (en) * | 2008-06-27 | 2011-04-21 | Olympus Corporation | Cell manipulation observation apparatus |
| CN102762713A (en) * | 2010-01-20 | 2012-10-31 | Emd密理博公司 | Cell image capturing and remote monitoring systems |
-
2014
- 2014-02-20 CN CN201410058742.XA patent/CN104862226A/en active Pending
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP4652801B2 (en) * | 2004-12-22 | 2011-03-16 | オリンパス株式会社 | Transmission illumination apparatus, microscope equipped with the same, and transmission illumination method |
| US20110091964A1 (en) * | 2008-06-27 | 2011-04-21 | Olympus Corporation | Cell manipulation observation apparatus |
| CN102762713A (en) * | 2010-01-20 | 2012-10-31 | Emd密理博公司 | Cell image capturing and remote monitoring systems |
Cited By (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106399048A (en) * | 2016-09-25 | 2017-02-15 | 东莞市联洲知识产权运营管理有限公司 | Improved microbiological incubator |
| CN106399049A (en) * | 2016-09-25 | 2017-02-15 | 东莞市联洲知识产权运营管理有限公司 | Lightproof microbiological incubator |
| CN106479878A (en) * | 2016-09-25 | 2017-03-08 | 东莞市联洲知识产权运营管理有限公司 | A kind of with removable micro- aobvious bio-incubator |
| CN112384606A (en) * | 2018-07-06 | 2021-02-19 | 北海道公立大学法人札幌医科大学 | Observation device |
| EP3819369A4 (en) * | 2018-07-06 | 2022-08-24 | Sapporo Medical University | Observation device |
| CN112384606B (en) * | 2018-07-06 | 2024-04-12 | 北海道公立大学法人札幌医科大学 | Viewing device |
| CN111534480A (en) * | 2019-11-01 | 2020-08-14 | 浙江大学 | In-vitro culture method of mammalian embryo |
| CN111534480B (en) * | 2019-11-01 | 2021-12-21 | 广州华珍生物科技有限公司 | In-vitro culture method of mammalian embryo |
| CN110747160A (en) * | 2019-11-27 | 2020-02-04 | 浙江大学 | High-survival-rate sheep fertilized egg culture method for extra-embryonic-body culture |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN104862226A (en) | Microbial incubator | |
| DK2173853T3 (en) | Device, system and method for monitoring and / or growing microscopic objects | |
| HRP20160791T1 (en) | Culture medium for epithelial stem cells and organoids comprising said stem cells | |
| WO2008104586A3 (en) | High surface cultivation system with surface increasing substrate | |
| CN205241704U (en) | Intelligent long -range observation monitoring devices of cell culture case | |
| CN109486671A (en) | A kind of medical test microbial cultivation equipment | |
| CN202945247U (en) | Human embryo culture vessel with single culture, co-culture and group culture advantages | |
| CN103442557A (en) | Method for preserving cells and cell cultures | |
| CN101851578A (en) | In-vitro multicellular line coculture dish and manufacturing method thereof | |
| CN201587946U (en) | In vitro co-culture apparatus for multiple types of cells | |
| CN204097488U (en) | Medical test cultivates test box with viable bacteria | |
| CN105199955A (en) | Three-dimensional cell culture device capable of performing mechanical-chemical coupling loading | |
| CN204737966U (en) | Multiple cell culture apparatus | |
| CN108130262A (en) | A kind of Micro-Organism Culture Dish of pathological experiment | |
| CN205473829U (en) | Interact's culture apparatus between cell culture and cell | |
| CN205398638U (en) | Be used for mycotic artificial containers of aveC sudden change | |
| CN204342806U (en) | Laser scanning co-focusing microscope special various kinds of cell Dual culture ware | |
| CN103620018B (en) | Cell cultivator | |
| CN109207363A (en) | Laminar-flow type living cells culture device | |
| CN204579394U (en) | A kind of soybean seedling nursing device | |
| CN205035391U (en) | Cell culture dish | |
| CN204550583U (en) | A kind of microbial detection device | |
| CN205511179U (en) | Plant incubator for experiments | |
| CN206428271U (en) | Embryo Culture ware | |
| CN205576165U (en) | Modified cell culture bottle |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| RJ01 | Rejection of invention patent application after publication |
Application publication date: 20150826 |
|
| RJ01 | Rejection of invention patent application after publication |