CN109207247A - The preparation method of antarctic krill oil, deodorant antarctic krill oil and its application, food - Google Patents
The preparation method of antarctic krill oil, deodorant antarctic krill oil and its application, food Download PDFInfo
- Publication number
- CN109207247A CN109207247A CN201811116201.2A CN201811116201A CN109207247A CN 109207247 A CN109207247 A CN 109207247A CN 201811116201 A CN201811116201 A CN 201811116201A CN 109207247 A CN109207247 A CN 109207247A
- Authority
- CN
- China
- Prior art keywords
- krill oil
- antarctic krill
- refining
- shrimp sauce
- deodorant
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
- 229940106134 krill oil Drugs 0.000 title claims abstract description 117
- 239000002781 deodorant agent Substances 0.000 title claims abstract description 48
- 238000002360 preparation method Methods 0.000 title claims abstract description 31
- 235000013305 food Nutrition 0.000 title claims abstract description 17
- 241000238557 Decapoda Species 0.000 claims abstract description 99
- 235000015067 sauces Nutrition 0.000 claims abstract description 84
- 238000000034 method Methods 0.000 claims abstract description 63
- 238000007670 refining Methods 0.000 claims abstract description 57
- 241000239366 Euphausiacea Species 0.000 claims abstract description 48
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 claims description 139
- 235000019441 ethanol Nutrition 0.000 claims description 81
- 229960004756 ethanol Drugs 0.000 claims description 57
- 239000007788 liquid Substances 0.000 claims description 41
- 238000000605 extraction Methods 0.000 claims description 39
- 230000008569 process Effects 0.000 claims description 32
- 239000002904 solvent Substances 0.000 claims description 28
- VLKZOEOYAKHREP-UHFFFAOYSA-N n-Hexane Chemical compound CCCCCC VLKZOEOYAKHREP-UHFFFAOYSA-N 0.000 claims description 26
- CSCPPACGZOOCGX-UHFFFAOYSA-N Acetone Chemical compound CC(C)=O CSCPPACGZOOCGX-UHFFFAOYSA-N 0.000 claims description 25
- 239000000287 crude extract Substances 0.000 claims description 23
- 239000000843 powder Substances 0.000 claims description 21
- 241000239370 Euphausia superba Species 0.000 claims description 19
- 150000001298 alcohols Chemical class 0.000 claims description 18
- 150000001335 aliphatic alkanes Chemical class 0.000 claims description 17
- 238000005119 centrifugation Methods 0.000 claims description 16
- 150000002576 ketones Chemical class 0.000 claims description 16
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 14
- 238000009835 boiling Methods 0.000 claims description 12
- 230000008719 thickening Effects 0.000 claims description 12
- 102000004190 Enzymes Human genes 0.000 claims description 11
- 108090000790 Enzymes Proteins 0.000 claims description 11
- 239000011449 brick Substances 0.000 claims description 11
- 230000009849 deactivation Effects 0.000 claims description 11
- 229960000935 dehydrated alcohol Drugs 0.000 claims description 11
- 238000001035 drying Methods 0.000 claims description 10
- 238000000926 separation method Methods 0.000 claims description 7
- 238000004090 dissolution Methods 0.000 claims description 6
- 238000009777 vacuum freeze-drying Methods 0.000 claims description 6
- 239000000463 material Substances 0.000 claims description 5
- 238000010348 incorporation Methods 0.000 claims description 3
- 238000010257 thawing Methods 0.000 claims description 3
- 239000003921 oil Substances 0.000 claims 4
- 150000003904 phospholipids Chemical class 0.000 abstract description 15
- 210000000697 sensory organ Anatomy 0.000 abstract description 8
- 239000000284 extract Substances 0.000 abstract description 7
- OKKJLVBELUTLKV-UHFFFAOYSA-N Methanol Chemical compound OC OKKJLVBELUTLKV-UHFFFAOYSA-N 0.000 description 15
- 238000002156 mixing Methods 0.000 description 13
- 239000000047 product Substances 0.000 description 13
- 238000001914 filtration Methods 0.000 description 11
- 238000012545 processing Methods 0.000 description 10
- 239000004615 ingredient Substances 0.000 description 9
- 239000000126 substance Substances 0.000 description 9
- 230000000052 comparative effect Effects 0.000 description 8
- DNJIEGIFACGWOD-UHFFFAOYSA-N ethanethiol Chemical compound CCS DNJIEGIFACGWOD-UHFFFAOYSA-N 0.000 description 8
- 239000012535 impurity Substances 0.000 description 8
- 238000003756 stirring Methods 0.000 description 8
- ZWEHNKRNPOVVGH-UHFFFAOYSA-N 2-Butanone Chemical compound CCC(C)=O ZWEHNKRNPOVVGH-UHFFFAOYSA-N 0.000 description 6
- ATUOYWHBWRKTHZ-UHFFFAOYSA-N Propane Chemical compound CCC ATUOYWHBWRKTHZ-UHFFFAOYSA-N 0.000 description 6
- 230000000694 effects Effects 0.000 description 6
- 235000020673 eicosapentaenoic acid Nutrition 0.000 description 6
- 239000012982 microporous membrane Substances 0.000 description 6
- BDERNNFJNOPAEC-UHFFFAOYSA-N propan-1-ol Chemical compound CCCO BDERNNFJNOPAEC-UHFFFAOYSA-N 0.000 description 6
- 238000001291 vacuum drying Methods 0.000 description 6
- 230000005484 gravity Effects 0.000 description 5
- 239000003960 organic solvent Substances 0.000 description 5
- 230000003647 oxidation Effects 0.000 description 5
- 238000007254 oxidation reaction Methods 0.000 description 5
- 238000005057 refrigeration Methods 0.000 description 5
- 150000003839 salts Chemical class 0.000 description 5
- LRHPLDYGYMQRHN-UHFFFAOYSA-N N-Butanol Chemical compound CCCCO LRHPLDYGYMQRHN-UHFFFAOYSA-N 0.000 description 4
- 238000001514 detection method Methods 0.000 description 4
- 238000004519 manufacturing process Methods 0.000 description 4
- 238000003672 processing method Methods 0.000 description 4
- 102000004169 proteins and genes Human genes 0.000 description 4
- 108090000623 proteins and genes Proteins 0.000 description 4
- UFTFJSFQGQCHQW-UHFFFAOYSA-N triformin Chemical compound O=COCC(OC=O)COC=O UFTFJSFQGQCHQW-UHFFFAOYSA-N 0.000 description 4
- OTMSDBZUPAUEDD-UHFFFAOYSA-N Ethane Chemical compound CC OTMSDBZUPAUEDD-UHFFFAOYSA-N 0.000 description 3
- 239000001273 butane Substances 0.000 description 3
- 239000003153 chemical reaction reagent Substances 0.000 description 3
- 235000013373 food additive Nutrition 0.000 description 3
- 239000002778 food additive Substances 0.000 description 3
- IJDNQMDRQITEOD-UHFFFAOYSA-N n-butane Chemical compound CCCC IJDNQMDRQITEOD-UHFFFAOYSA-N 0.000 description 3
- OFBQJSOFQDEBGM-UHFFFAOYSA-N n-pentane Natural products CCCCC OFBQJSOFQDEBGM-UHFFFAOYSA-N 0.000 description 3
- 235000016709 nutrition Nutrition 0.000 description 3
- 239000001294 propane Substances 0.000 description 3
- 239000002994 raw material Substances 0.000 description 3
- 235000021122 unsaturated fatty acids Nutrition 0.000 description 3
- 150000004670 unsaturated fatty acids Chemical class 0.000 description 3
- JEBFVOLFMLUKLF-IFPLVEIFSA-N Astaxanthin Natural products CC(=C/C=C/C(=C/C=C/C1=C(C)C(=O)C(O)CC1(C)C)/C)C=CC=C(/C)C=CC=C(/C)C=CC2=C(C)C(=O)C(O)CC2(C)C JEBFVOLFMLUKLF-IFPLVEIFSA-N 0.000 description 2
- OAICVXFJPJFONN-UHFFFAOYSA-N Phosphorus Chemical compound [P] OAICVXFJPJFONN-UHFFFAOYSA-N 0.000 description 2
- 238000013459 approach Methods 0.000 description 2
- MQZIGYBFDRPAKN-ZWAPEEGVSA-N astaxanthin Chemical compound C([C@H](O)C(=O)C=1C)C(C)(C)C=1/C=C/C(/C)=C/C=C/C(/C)=C/C=C/C=C(C)C=CC=C(C)C=CC1=C(C)C(=O)[C@@H](O)CC1(C)C MQZIGYBFDRPAKN-ZWAPEEGVSA-N 0.000 description 2
- 235000013793 astaxanthin Nutrition 0.000 description 2
- 229940022405 astaxanthin Drugs 0.000 description 2
- 239000001168 astaxanthin Substances 0.000 description 2
- 230000008901 benefit Effects 0.000 description 2
- 230000008859 change Effects 0.000 description 2
- 150000001875 compounds Chemical class 0.000 description 2
- 239000012043 crude product Substances 0.000 description 2
- 235000013365 dairy product Nutrition 0.000 description 2
- 238000011161 development Methods 0.000 description 2
- 230000018109 developmental process Effects 0.000 description 2
- 238000005516 engineering process Methods 0.000 description 2
- 230000002255 enzymatic effect Effects 0.000 description 2
- 238000011156 evaluation Methods 0.000 description 2
- 239000012530 fluid Substances 0.000 description 2
- 239000006260 foam Substances 0.000 description 2
- 238000004108 freeze drying Methods 0.000 description 2
- 238000002955 isolation Methods 0.000 description 2
- 150000002632 lipids Chemical class 0.000 description 2
- 235000012054 meals Nutrition 0.000 description 2
- 230000004048 modification Effects 0.000 description 2
- 238000012986 modification Methods 0.000 description 2
- 230000035764 nutrition Effects 0.000 description 2
- 229910052698 phosphorus Inorganic materials 0.000 description 2
- 239000011574 phosphorus Substances 0.000 description 2
- 230000001953 sensory effect Effects 0.000 description 2
- 239000002028 Biomass Substances 0.000 description 1
- OKTJSMMVPCPJKN-UHFFFAOYSA-N Carbon Chemical compound [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 description 1
- 241000195493 Cryptophyta Species 0.000 description 1
- -1 Ethyl alcohol Chemical compound 0.000 description 1
- AFCARXCZXQIEQB-UHFFFAOYSA-N N-[3-oxo-3-(2,4,6,7-tetrahydrotriazolo[4,5-c]pyridin-5-yl)propyl]-2-[[3-(trifluoromethoxy)phenyl]methylamino]pyrimidine-5-carboxamide Chemical compound O=C(CCNC(=O)C=1C=NC(=NC=1)NCC1=CC(=CC=C1)OC(F)(F)F)N1CC2=C(CC1)NN=N2 AFCARXCZXQIEQB-UHFFFAOYSA-N 0.000 description 1
- 229930182558 Sterol Natural products 0.000 description 1
- 239000002253 acid Substances 0.000 description 1
- 230000004913 activation Effects 0.000 description 1
- JAZBEHYOTPTENJ-JLNKQSITSA-N all-cis-5,8,11,14,17-icosapentaenoic acid Chemical compound CC\C=C/C\C=C/C\C=C/C\C=C/C\C=C/CCCC(O)=O JAZBEHYOTPTENJ-JLNKQSITSA-N 0.000 description 1
- 230000000844 anti-bacterial effect Effects 0.000 description 1
- 230000001093 anti-cancer Effects 0.000 description 1
- 230000003110 anti-inflammatory effect Effects 0.000 description 1
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 239000008280 blood Substances 0.000 description 1
- 210000004369 blood Anatomy 0.000 description 1
- 230000008499 blood brain barrier function Effects 0.000 description 1
- 230000036772 blood pressure Effects 0.000 description 1
- 210000001218 blood-brain barrier Anatomy 0.000 description 1
- 210000004556 brain Anatomy 0.000 description 1
- 229910052799 carbon Inorganic materials 0.000 description 1
- 239000003795 chemical substances by application Substances 0.000 description 1
- WORJEOGGNQDSOE-UHFFFAOYSA-N chloroform;methanol Chemical compound OC.ClC(Cl)Cl WORJEOGGNQDSOE-UHFFFAOYSA-N 0.000 description 1
- 238000007796 conventional method Methods 0.000 description 1
- 235000014113 dietary fatty acids Nutrition 0.000 description 1
- 229960005135 eicosapentaenoic acid Drugs 0.000 description 1
- JAZBEHYOTPTENJ-UHFFFAOYSA-N eicosapentaenoic acid Natural products CCC=CCC=CCC=CCC=CCC=CCCCC(O)=O JAZBEHYOTPTENJ-UHFFFAOYSA-N 0.000 description 1
- 229930195729 fatty acid Natural products 0.000 description 1
- 239000000194 fatty acid Substances 0.000 description 1
- 150000004665 fatty acids Chemical class 0.000 description 1
- 230000036541 health Effects 0.000 description 1
- 235000003642 hunger Nutrition 0.000 description 1
- 230000002218 hypoglycaemic effect Effects 0.000 description 1
- 231100000053 low toxicity Toxicity 0.000 description 1
- 239000012528 membrane Substances 0.000 description 1
- 210000005036 nerve Anatomy 0.000 description 1
- 235000020660 omega-3 fatty acid Nutrition 0.000 description 1
- 229910052760 oxygen Inorganic materials 0.000 description 1
- 239000001301 oxygen Substances 0.000 description 1
- 238000007781 pre-processing Methods 0.000 description 1
- 230000002265 prevention Effects 0.000 description 1
- 235000020995 raw meat Nutrition 0.000 description 1
- 238000011084 recovery Methods 0.000 description 1
- 230000028327 secretion Effects 0.000 description 1
- 230000009758 senescence Effects 0.000 description 1
- 150000003432 sterols Chemical class 0.000 description 1
- 235000003702 sterols Nutrition 0.000 description 1
- 238000012360 testing method Methods 0.000 description 1
- 231100000419 toxicity Toxicity 0.000 description 1
- 230000001988 toxicity Effects 0.000 description 1
- 235000013619 trace mineral Nutrition 0.000 description 1
- 239000011573 trace mineral Substances 0.000 description 1
- 208000019553 vascular disease Diseases 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C11—ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
- C11B—PRODUCING, e.g. BY PRESSING RAW MATERIALS OR BY EXTRACTION FROM WASTE MATERIALS, REFINING OR PRESERVING FATS, FATTY SUBSTANCES, e.g. LANOLIN, FATTY OILS OR WAXES; ESSENTIAL OILS; PERFUMES
- C11B1/00—Production of fats or fatty oils from raw materials
- C11B1/02—Pretreatment
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
- A23L33/10—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
- A23L33/115—Fatty acids or derivatives thereof; Fats or oils
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L5/00—Preparation or treatment of foods or foodstuffs, in general; Food or foodstuffs obtained thereby; Materials therefor
- A23L5/20—Removal of unwanted matter, e.g. deodorisation or detoxification
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L5/00—Preparation or treatment of foods or foodstuffs, in general; Food or foodstuffs obtained thereby; Materials therefor
- A23L5/20—Removal of unwanted matter, e.g. deodorisation or detoxification
- A23L5/21—Removal of unwanted matter, e.g. deodorisation or detoxification by heating without chemical treatment, e.g. steam treatment, cooking
-
- C—CHEMISTRY; METALLURGY
- C11—ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
- C11B—PRODUCING, e.g. BY PRESSING RAW MATERIALS OR BY EXTRACTION FROM WASTE MATERIALS, REFINING OR PRESERVING FATS, FATTY SUBSTANCES, e.g. LANOLIN, FATTY OILS OR WAXES; ESSENTIAL OILS; PERFUMES
- C11B1/00—Production of fats or fatty oils from raw materials
- C11B1/10—Production of fats or fatty oils from raw materials by extracting
-
- C—CHEMISTRY; METALLURGY
- C11—ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
- C11B—PRODUCING, e.g. BY PRESSING RAW MATERIALS OR BY EXTRACTION FROM WASTE MATERIALS, REFINING OR PRESERVING FATS, FATTY SUBSTANCES, e.g. LANOLIN, FATTY OILS OR WAXES; ESSENTIAL OILS; PERFUMES
- C11B3/00—Refining fats or fatty oils
- C11B3/001—Refining fats or fatty oils by a combination of two or more of the means hereafter
-
- C—CHEMISTRY; METALLURGY
- C11—ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
- C11B—PRODUCING, e.g. BY PRESSING RAW MATERIALS OR BY EXTRACTION FROM WASTE MATERIALS, REFINING OR PRESERVING FATS, FATTY SUBSTANCES, e.g. LANOLIN, FATTY OILS OR WAXES; ESSENTIAL OILS; PERFUMES
- C11B3/00—Refining fats or fatty oils
- C11B3/006—Refining fats or fatty oils by extraction
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2002/00—Food compositions, function of food ingredients or processes for food or foodstuffs
Landscapes
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Oil, Petroleum & Natural Gas (AREA)
- Wood Science & Technology (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Organic Chemistry (AREA)
- Microbiology (AREA)
- Health & Medical Sciences (AREA)
- Nutrition Science (AREA)
- Food Science & Technology (AREA)
- Polymers & Plastics (AREA)
- Mycology (AREA)
- Meat, Egg Or Seafood Products (AREA)
- Medicines Containing Material From Animals Or Micro-Organisms (AREA)
Abstract
The invention discloses a kind of preparation method of antarctic krill oil, deodorant antarctic krill oil and its applications, food, are related to krill oil extractive technique field.The preparation method of antarctic krill oil is the following steps are included: the antarctic krill oil progress defishying that krill is obtained after extracted, obtains deodorant antarctic krill oil；Wherein, defishying includes: dry 1-24h under the conditions of vacuum degree 0.02-0.15MPa, 20-60 DEG C；Extract includes slightly mentioning and optional refining.The present invention can effectively remove the bad smell and dissolvent residual of krill oil, remove volatile component 0.5-2.5% compared with antarctic krill oil before defishying after defishying, and shrimp sauce is substantially without bad smell on sense organ.The method of the present invention carries out phosphatide enrichment preferably before deodorant, can further prepare the antarctic krill oil of high phospholipid content.
Description
Technical field
The present invention relates to krill oil extractive technique field, in particular to a kind of preparation method, de- of antarctic krill oil
Raw meat antarctic krill oil and its application, food.
Background technique
Krill is a kind of large krill kind for being distributed in ring South Pole sea area, and total biomass is about hundred million tons of 6.5-10, year
Can amount of fishing be about hundred million tons of 0.13-1.Krill is made a living with the algae that ingests, rich in protein, Ω -3 unsaturated fatty acid,
A variety of nutriments such as phosphatide, astaxanthin, sterol.The lipid matter that preparation is extracted in definition at present by raw material of krill is south
Pole krill oil.Antarctic krill oil is rich in Ω -3 polyunsaturated fatty acid, phosphatide, astaxanthin and various trace elements, and its insatiable hunger
Exist in the form of the compound molecule of phosphatide with fatty acid, is a kind of rare natural work with superpower oxidation resistance of nature
Property multi-effect compound molecule, great Development volue.
Antarctic krill oil extractive technique is broadly divided into three kinds of methods: organic solvent method, supercritical methanol technology and enzymatic isolation method at present.Have
Solvent method is to be extracted with n-hexane, chloroform methanol, acetone, No. 6 gasoline, ethyl alcohol etc. for solvent, have recovery rate it is high, at
This low feature, but have certain dissolvent residual, it causes potentially to eat toxicity.Supercritical methanol technology utilizes CO2Under superpressure state
Being formed, there is the supercritical fluid of liquid property to be extracted, and have the characteristics that no solvent residue, but supercritical CO2Fluid extraction
Shrimp sauce content of phospholipid it is low, and this method equipment operation is complicated, high production cost.Enzymatic isolation method is generally original with the wet shrimp of krill
Material, eliminates vacuum drying pretreatment process, but the thick shrimp sauce impurity extracted is more, increase subsequent refining difficulty and at
This.In addition, antarctic krill oil is either slightly mentioned or refined, there is certain shrimp sauce bad smell.
It is therefore desired to provide a kind of preparation method of improved antarctic krill oil, it is able to solve in the above problem
At least one.
In view of this, the present invention is specifically proposed.
Summary of the invention
One of the objects of the present invention is to provide a kind of preparation methods of antarctic krill oil, are able to solve in the above problem
At least one.
The second object of the present invention is to provide a kind of deodorant antarctic krill oil, using the preparation side of above-mentioned antarctic krill oil
Method is prepared, and bad smell is small on shrimp sauce sense organ.
The third object of the present invention is to provide a kind of above-mentioned deodorant antarctic krill oil and is preparing the application in food.
The fourth object of the present invention is to provide a kind of food, be mainly prepared by above-mentioned deodorant antarctic krill oil have
There is advantage identical with above-mentioned deodorant antarctic krill oil.
In order to realize above-mentioned purpose of the invention, the following technical scheme is adopted:
In a first aspect, providing a kind of preparation method of antarctic krill oil, comprising the following steps:
The antarctic krill oil that krill is obtained after extracted carries out defishying, obtains deodorant antarctic krill oil；
Wherein, defishying includes: dry 1-24h under the conditions of vacuum degree 0.02-0.15MPa, 20-60 DEG C；Extract packet
It includes and slightly mentions and optional refining.
Preferably, on the basis of technical solution provided by the invention, the vacuum degree of defishying is 0.05-0.1MPa,
Drying temperature is 20-30 DEG C, drying time 12-24h.
Preferably, on the basis of technical solution provided by the invention, slightly mention the following steps are included:
Coarse extraction and separation are carried out to krill with thick extraction solvent, obtain crude extract, crude extract is concentrated, obtains thick shrimp
Oil；
Preferably, thick extraction solvent includes one in the alcohols of C1-C4, the ketone of C1-C4, the alkanes of C1-C6 or No. 6 gasoline
Kind is several, preferably one or more of the alcohols of C1-C4 or the alkanes of C1-C6, preferably ethyl alcohol or n-hexane, into one
Step is preferably ethyl alcohol；
Preferably, the water content of ethyl alcohol is 0-20%；
Preferably, it is 20-30 DEG C, extraction time 1-3h that the process conditions of coarse extraction, which include: Extracting temperature, and solid-liquid ratio is
1:5-1:10g/mL；
Preferably, it is concentrated as vacuum concentration；Preferably, it is 35-80 that the process conditions of vacuum concentration, which include: thickening temperature,
DEG C, vacuum degree 0.09-0.1MPa.
Preferably, on the basis of technical solution provided by the invention, refining the following steps are included:
It repeats step (I) 2-3 times, obtains refining shrimp sauce；Step (I) includes: with refining solvent to the thick shrimp sauce after slightly mentioning
Heavy molten and separation is carried out, refining liquid is obtained, is then concentrated in vacuo refining liquid；
Preferably, refining solvent includes one in the alcohols of C1-C4, the ketone of C1-C4, the alkanes of C1-C6 or No. 6 gasoline
Kind is several, preferably one or more of the alcohols of C1-C4 or the alkanes of C1-C6, preferably ethyl alcohol or n-hexane, into one
Step is preferably dehydrated alcohol；
Preferably, weight is molten is carried out in the case where strength mixes, and the molten process conditions of weight include: that incorporation time is 20-
40min, solid-liquid ratio 1:20-1:50g/mL；
Preferably, 1-2h, then isolated refining liquid are stood after weight is molten；
Preferably, it is concentrated as vacuum concentration；Preferably, it is 35-80 that the process conditions of vacuum concentration, which include: thickening temperature,
DEG C, vacuum degree 0.09-0.1MPa.
Preferably, on the basis of technical solution provided by the invention, the krill is pretreated South Pole phosphorus
Shrimp med；
Preferably, pretreatment the following steps are included:
After the krill shrimp brick of frost is thawed, boiling enzyme deactivation is living, crushes after vacuum freeze drying, obtains krill
Powder.
Preferably, on the basis of technical solution provided by the invention, the method also includes: krill is extracted
The antarctic krill oil obtained afterwards first carries out the step of phosphatide enrichment carries out defishying again；
Preferably, phosphatide enrichment the following steps are included:
The krill after extraction is sufficiently dissolved and separated with solvent-rich, obtains enrichment shrimp sauce；
Preferably, solvent-rich includes one in the alcohols of C1-C4, the ketone of C1-C4, the alkanes of C1-C6 or No. 6 gasoline
Kind is several, preferably one or more of the alcohols of C1-C4 or the ketone of C1-C4, preferably ethyl alcohol or acetone, further
Preferably ethyl alcohol；
Preferably, separate mode is centrifugation；Preferably, centrifugation rate 4000-6000rpm, centrifugation time 10-15min.
Preferably, on the basis of technical solution provided by the invention, the preparation method of antarctic krill oil includes following step
It is rapid:
(a) after the krill shrimp brick of frost thawing, boiling enzyme deactivation is living, crushes after vacuum freeze drying, obtains the South Pole
Krill meal；
(b) coarse extraction and separation are carried out to euphausia superba powder with the ethyl alcohol of water content 0-20%, obtains crude extract, crude extract
It is concentrated in vacuo, obtains thick shrimp sauce；
The process conditions of coarse extraction include: that Extracting temperature is 20-30 DEG C, extraction time 1-3h, solid-liquid ratio 1:5-1:
10g/mL；
The process conditions of vacuum concentration include: that thickening temperature is 35-80 DEG C, vacuum degree 0.09-0.1MPa；
(c) molten, the solid-liquid ratio 1:20-1:50g/mL of weight, mixing are carried out in the case where strength mixes to thick shrimp sauce with dehydrated alcohol
Time is 20-40min, stands 1-2h, then isolated refining liquid after weight is molten；Refining liquid is concentrated in vacuo, is concentrated in vacuo
Process conditions include: thickening temperature be 35-80 DEG C, vacuum degree 0.09-0.1MPa；
(d) it repeats step (c) 2-3 times, obtains refining shrimp sauce；
(e) refining shrimp sauce is dissolved with ethyl alcohol, is centrifuged after completely dissolution, centrifugation rate 4000-6000rpm, from
Heart time 10-15min obtains enrichment shrimp sauce；
(f) it will be enriched with shrimp sauce dry 2-24h under the conditions of vacuum degree 0.05-0.1MPa, 25-60 DEG C, obtain the deodorant South Pole
Krill oil.
Second aspect is provided a kind of deodorant antarctic krill oil, is prepared into using the preparation method of above-mentioned antarctic krill oil
It arrives.
The third aspect provides a kind of above-mentioned deodorant antarctic krill oil and is preparing the application in food.
Fourth aspect provides a kind of food, is mainly prepared by above-mentioned deodorant antarctic krill oil.
Compared with the prior art, the invention has the following beneficial effects:
(1) krill is slightly mentioned and is obtained after optionally refining by the preparation method of antarctic krill oil of the invention
Antarctic krill oil carries out vacuum drying defishying, and dry 1-24h comes under the conditions of vacuum degree 0.02-0.15MPa, 20-60 DEG C
It completes, this method simple process is convenient, the bad smell and dissolvent residual of krill oil can be effectively removed, compared with deodorant after defishying
Antarctic krill oil removes volatile component 0.5-2.5% before handling, and shrimp sauce is substantially without bad smell on sense organ.The present invention alleviates south
Pole krill oil preferably improves its city because bad smell interferes consumer edible or technical bottleneck problem for food additives
Field application approach.
(2) preparation method of preferred antarctic krill oil includes pre-processing, slightly mentioning, refining removal of impurities, phosphatide enrichment and deodorant
Step, wherein slightly mention, refine the several steps such as removal of impurities, phosphatide enrichment to handle using addition organic solvent, organic solvent
That selects the license of the country such as ethyl alcohol, n-hexane, acetone can be used for food service industry low toxicity or innoxious solvent.Wherein, ethyl alcohol can be used
It slightly mentions, refine as the realization of unique organic solvent, the integral manufacturing technique of phosphatide enrichment.This typical method not only may be used
To prepare the antarctic krill oil of high phospholipid content, and it can effectively remove the bad smell and dissolvent residual of krill oil.Simultaneously
The process conditions such as whole process temperature are suitable for that will not damage to the nutritional ingredient of krill oil, and antarctic krill oil has outstanding
Oxidation stability, superior product quality.
Detailed description of the invention
In order to illustrate the technical solution of the embodiments of the present invention more clearly, below will be to needed in the embodiment attached
Figure is briefly described, it should be understood that the following drawings illustrates only certain embodiments of the present invention, therefore is not construed as pair
The restriction of range for those of ordinary skill in the art without creative efforts, can also be according to this
A little attached drawings obtain other relevant attached drawings.
Fig. 1 is the antarctic krill oil after 3 refining treatment of the embodiment of the present invention.
Specific embodiment
Embodiment of the present invention is described in detail below in conjunction with embodiment, but those skilled in the art will
Understand, the following example is merely to illustrate the present invention, and is not construed as limiting the scope of the invention.It is not specified in embodiment specific
Condition person carries out according to conventional conditions or manufacturer's recommended conditions.Reagents or instruments used without specified manufacturer is
The conventional products that can be obtained by commercially available purchase.
According to the first aspect of the invention, a kind of preparation method of antarctic krill oil is provided, comprising the following steps: will
The antarctic krill oil obtained after krill is extracted carries out defishying, obtains deodorant antarctic krill oil；Wherein, defishying
It include: dry 1-24h under the conditions of vacuum degree 0.02-0.15MPa, 20-60 DEG C；Extract includes slightly mentioning and optional refining.
The source of krill is not construed as limiting, preprocessed (such as crushing after krill freeze-drying) can be used
The euphausia superba powder of acquisition.
In the disclosure " extraction " include slightly mention with optional refining, i.e., " antarctic krill oil obtained after extraction ", which refers to, is slightly mentioned
The antarctic krill oil obtained afterwards or the antarctic krill oil for slightly being mentioned and being obtained after refining.
Slightly mention is the process tentatively extracted (primary to extract) to krill using extractant and obtain crude product, this field
Technical staff can be specifically chosen the conditions such as suitable extractant, Extracting temperature and extraction time according to the actual situation.Refining is
Crude product weight is molten and again extract the process of (second extraction) again, those skilled in the art can according to the actual situation, specific choosing
Select the conditions such as suitable solution temperature, solvent and Extracting temperature.
Slightly mentioning can be carried out with refining process using the technique and condition of this field routine.
Antarctic krill oil after extraction is carried out defishying by the disclosure, obtains deodorant antarctic krill oil.
Defishying is to carry out vacuum drying to krill oil to remove stench substance and dissolvent residual.Here krill oil can be thick
Thick shrimp sauce after mentioning, the refining shrimp sauce after being also possible to refining.
Vacuum degree 0.02-0.15MPa, it is typical but non-limiting be, for example, 0.02MPa, 0.03MPa, 0.04MPa,
0.05MPa, 0.06MPa, 0.08MPa, 0.1MPa, 0.12MPa, 0.13MPa, 0.14MPa or 0.15MPa.Treatment temperature 20-60
DEG C, typical but non-limiting is, for example, 20 DEG C, 25 DEG C, 30 DEG C, 35 DEG C, 40 DEG C, 45 DEG C, 50 DEG C, 55 DEG C or 60 DEG C.When dry
Between 1-24h, typical but non-limiting is, for example, 1h, 2h, 4h, 6h, 8h, 10h, 12h, 14h, 16h, 18h, 20h or for 24 hours.
Temperature removes volatile more when being 60 DEG C, but cause certain ingredient oxygenolysis in shrimp sauce raw because temperature is higher
The volatile materials of Cheng Xin causes bad smell after vacuum deodorant not drop to ideal range, and phosphatide easy oxygen when being higher than 60 DEG C
Change blackening, therefore the temperature highest of vacuum deodorant selects 60 DEG C.
Preferably, a certain amount of antarctic krill oil is taken to be placed in certain open containers when processing, it is preferable that laboratory is selected
Conical flask.Because antarctic krill oil has volatile materials and oil components to form foam and in low-voltage ring during vacuum drying
Border float downward, to exempt from foam overflow, preferably conical flask volume (ml): krill oil quality (g) >=10:1.
Preferably, select vacuum oven as vacuum equipment, but be not limited to vacuum oven, can be it is other can be with
The equipment of condition of high vacuum degree is provided.
The present invention carries out vacuum drying defishying, in vacuum degree 0.02- on the basis of antarctic krill oil produces
1-24h is dried under the conditions of 0.15MPa, 20-60 DEG C to complete, this method simple process is convenient, can effectively remove krill oil
Bad smell and dissolvent residual remove volatile component 0.5-2.5%, sense organ compared with antarctic krill oil before defishying after defishying
Upper shrimp sauce bad smell does not have or very light substantially.The present invention alleviates antarctic krill oil because bad smell interferes consumer edible or is used for
The technical bottleneck problem of food additives preferably improves its market application approach and health care benefit.
In a preferred embodiment, the vacuum degree of defishying is 0.05-0.1MPa, drying temperature 20-30
DEG C, drying time 12-24h.
By optimizing defishying technological parameter, it can further be promoted and extract deodorant stink and the effect except dissolvent residual.
In a preferred embodiment, it slightly mentions the following steps are included: slightly being mentioned with thick extraction solvent to krill
It takes and separates, obtain crude extract, crude extract is concentrated, obtains thick shrimp sauce.
Thick extraction solvent typical but non-limiting for example, alcohols (methanol, ethyl alcohol, propyl alcohol, butanol), the C1-C4 of C1-C4
Ketone (acetone, butanone etc.), C1-C6 alkanes (ethane, propane, butane, n-hexane) or one of No. 6 gasoline or several
Kind, preferably one or more of the alcohols of C1-C4 or the alkanes of C1-C6, preferably ethyl alcohol or n-hexane, further preferably
For ethyl alcohol.
One or more expression can be the mixing of several alcohol or several ketone or several alkane, be also possible to the differences such as alcohol, ketone
The mixing of type.
Here the water content of ethyl alcohol be 0-20% (water volume accounts for the ratio between water and ethyl alcohol total volume), such as dehydrated alcohol,
95% ethyl alcohol (VEthyl alcohol:VWater=95:5), 90% ethyl alcohol, 85% ethyl alcohol, 80% ethyl alcohol.
Preferably, it is 20-30 DEG C, extraction time 1-3h that the process conditions of coarse extraction, which include: Extracting temperature, and solid-liquid ratio is
1:5-1:10g/mL。
Typical but non-limiting Extracting temperature is, for example, 20 DEG C, 25 DEG C or 30 DEG C, preferably 25 DEG C.Extraction time it is typical but
Unrestricted is, for example, 1h, 2h or 3h.The ratio between solid-liquid ratio south pole krill quality and thick extraction solvent volume.Solid-liquid ratio it is typical but
Unrestricted is, for example, 1:5g/mL, 1:6g/mL, 1:7g/mL, 1:8g/mL, 1:9g/mL or 1:10g/mL.
Be stirred when coarse extraction, stirring rate 500-1000rpm, for example, 500rpm, 600rpm, 700rpm,
800rpm, 900rpm or 1000rpm.
Preferably, the mixed liquor obtained after coarse extraction is handled with filtering with microporous membrane, preferably 0.22 μm of miillpore filter
Aperture filter membrane, obtains crude extract.
Preferably, crude extract is concentrated in vacuo, it is preferable that the thickening temperature of vacuum concentration be 35-80 DEG C, such as 35 DEG C,
40 DEG C, 45 DEG C, 50 DEG C, 55 DEG C, 60 DEG C, 65 DEG C, 70 DEG C, 75 DEG C or 80 DEG C, vacuum degree 0.09-0.1MPa, such as
0.09MPa, 0.095MPa or 0.1MPa.
The yield of the thick shrimp sauce obtained after coarse extraction, vacuum concentration is in 8-15%.
In a preferred embodiment, refining the following steps are included: with refine solvent to the thick shrimp sauce after slightly mentioning into
Row weight is molten and separates, and obtains refining liquid, then refining liquid is concentrated；It repeats the above steps 2-3 times, obtains refining shrimp sauce.
Here " repeating the above steps " refers to that weight is molten to be made to concentration from being carried out with refining solvent to the thick shrimp sauce after slightly mentioning
It is primary.
Preferably, refining solvent typical but non-limiting for example, alcohols (methanol, ethyl alcohol, propyl alcohol, the fourth of C1-C4
Alcohol), the ketone (acetone, butanone etc.) of C1-C4, C1-C6 alkanes (ethane, propane, butane, n-hexane) or No. 6 gasoline in
One or more, preferably one or more of the alcohols of C1-C4 or the alkanes of C1-C6；
One or more expression can be the mixing of several alcohol or several ketone or several alkane, be also possible to the differences such as alcohol, ketone
The mixing of type.
Preferably ethyl alcohol or n-hexane, further preferably dehydrated alcohol.
It is dissolved in strength again and mixes lower progress, strength mixing refers to strong stirring, and revolving speed is generally in 5000rpm or more, it is preferable that
The strong stirring time is 20-40min, such as 20min, 30min or 40min.Solid-liquid ratio refers to thick shrimp sauce quality and refining solvent body
The ratio between product.Typical but non-limiting solid-liquid ratio is, for example, 1:20g/mL, 1:25g/mL, 1:30g/mL, 1:35g/mL, 1:40g/
ML or 1:50g/mL.
Preferably, 1-2h, then isolated refining liquid are stood after weight is molten；Isolated mode can be used and slightly be mentioned in step
Identical filtration treatment mode.
Preferably, refining liquid is concentrated in vacuo after processing.Preferably, the thickening temperature of vacuum concentration is 35-80 DEG C, example
Such as 35 DEG C, 40 DEG C, 45 DEG C, 50 DEG C, 55 DEG C, 60 DEG C, 65 DEG C, 70 DEG C, 75 DEG C or 80 DEG C, vacuum degree 0.09-0.1MPa, such as
0.09MPa, 0.095MPa or 0.1MPa.
Refining can effectively remove the components such as inorganic salts and protein, and imurity-removal ingredient accounts for the 6- of thick shrimp sauce specific gravity
15%.It is more limpid to refine the apparent color of shrimp sauce, refines unsaturated fatty acid content (eicosapentaenoic acid (EPA)+two in shrimp sauce
12 carbon, six diluted acid (DHA)) it is 20-30%, content of phospholipid 300-500mg/g.
In a preferred embodiment, krill is pretreated euphausia superba powder；Use euphausia superba powder
Be conducive to the extraction of the ingredients such as phosphatide.
Preferably, after pretreatment is the following steps are included: the krill shrimp brick of frost is thawed, boiling enzyme deactivation is living, vacuum
It is crushed after freeze-drying, obtains euphausia superba powder.
Boiling and vacuum freeze drying can be carried out using usual manner.
In a preferred embodiment, method further include: the antarctic krill oil for obtaining krill after extracted
First carry out the step of phosphatide enrichment carries out defishying again.
Extract include slightly mention with optional refining, i.e. method may include: slightly to propose-phosphatide enrichment-deodorant, or, slightly mention-smart
Refining-phosphatide enrichment-deodorant.
The antarctic krill oil of high phospholipid content can be obtained by phosphatide enrichment.
Preferably, phosphatide enrichment is the following steps are included: sufficiently dissolve the krill after extraction with solvent-rich
And separation, obtain enrichment shrimp sauce；
Solvent-rich typical but non-limiting for example, alcohols (methanol, ethyl alcohol, propyl alcohol, butanol), the C1-C4 of C1-C4
Ketone (acetone, butanone etc.), C1-C6 alkanes (ethane, propane, butane, n-hexane) or No. 6 gasoline one or more, it is excellent
It is selected as one or more of the alcohols of C1-C4 or the ketone of C1-C4, preferably ethyl alcohol or acetone, further preferably 30-
40% ethyl alcohol.
Preferably, separate mode is centrifugation；Preferably, centrifugation rate 4000-6000rpm, for example, 4000rpm,
5000rpm or 6000rpm, centrifugation time 10-15min.For example, 10min, 12min or 15min.
It is separated again by the way that solvent-rich weight is molten, removes triglyceride type antarctic krill oil, obtain high phospholipid content South Pole phosphorus
Shrimp sauce, yield unsaturated fatty acid content (EPA+ in the high phospholipid content antarctic krill oil after 60-90%, phosphatide enrichment
It DHA) is 15-45%, content of phospholipid 400-950mg/g.
Preferably, a kind of preparation method of typical antarctic krill oil, comprising the following steps:
(a) after the krill shrimp brick of frost thawing, boiling enzyme deactivation is living, crushes after vacuum freeze drying, obtains the South Pole
Krill meal；
(b) coarse extraction, separation are carried out to euphausia superba powder with the ethyl alcohol of water content 0-20%, obtains crude extract, crude extract
It is concentrated in vacuo, obtains thick shrimp sauce；
The process conditions of coarse extraction include: that Extracting temperature is 20-30 DEG C, extraction time 1-3h, solid-liquid ratio 1:5-1:
10g/mL；
The process conditions of vacuum concentration include: that thickening temperature is 35-80 DEG C, vacuum degree 0.09-0.1MPa；
(c) molten, the solid-liquid ratio 1:20-1:50g/mL of weight, mixing are carried out in the case where strength mixes to thick shrimp sauce with dehydrated alcohol
Time is 20-40min, stands 1-2h, then isolated refining liquid after weight is molten；Refining liquid is concentrated in vacuo, is concentrated in vacuo
Process conditions include: thickening temperature be 35-80 DEG C, vacuum degree 0.09-0.1MPa；
(d) it repeats step (c) 2-3 times, obtains refining shrimp sauce；
(e) refining shrimp sauce is dissolved with ethyl alcohol, is centrifuged after completely dissolution, centrifugation rate 4000-6000rpm, from
Heart time 10-15min obtains enrichment shrimp sauce；
(f) it will be enriched with shrimp sauce dry 2-24h under the conditions of vacuum degree 0.05-0.1MPa, 25-60 DEG C, obtain the deodorant South Pole
Krill oil.
The preparation method of the typical antarctic krill oil is slightly mentioned as the realization of unique organic solvent using ethyl alcohol, refines and remove
Miscellaneous, phosphatide enrichment integral manufacturing technique.This method can not only prepare the antarctic krill oil of high phospholipid content, Er Qieke
Effectively to remove the bad smell and dissolvent residual of krill oil.Whole process temperature condition is suitable simultaneously, will not be to the battalion of krill oil
It forms and point damages, antarctic krill oil has outstanding oxidation stability, improves product quality.
According to the second aspect of the invention, a kind of deodorant antarctic krill oil is provided, using above-mentioned antarctic krill oil
Preparation method is prepared.
Bad smell is small on the shrimp sauce sense organ prepared by the method for the invention.
According to the third aspect of the present invention, it provides a kind of above-mentioned deodorant antarctic krill oil and is preparing answering in food
With.
Food is for example including ready-mixed oil, drink, dairy produce, baking product or nutriment etc..
Since the antarctic krill oil bad smell that the method for the present invention obtains is small, consumer will not be interfered edible or for food additive
Add agent to use, therefore, prepares food using deodorant antarctic krill oil and have a extensive future.
According to the fourth aspect of the present invention, provide a kind of food, mainly by above-mentioned deodorant antarctic krill oil prepare and
At.
Food is for example including ready-mixed oil, drink, dairy produce, baking product or nutriment etc..
The food is rich in the nutrition of antarctic krill oil due to being prepared using the deodorant antarctic krill oil of the disclosure
Substance has superpower oxidation resistance, has reducing blood lipid, hypoglycemic, blood pressure lowering, passes through blood-brain barrier nutrition and activation brain is thin
Born of the same parents and nerve play prevention and treatment cranial vascular disease；Antibacterial anti-inflammatory, the occurrence and development of pre- anti-cancer delay senescence, in adjusting
The multi-efficiencies such as secretion.
In order to further appreciate that the present invention, the method for the present invention and effect are done further in detail combined with specific embodiments below
Explanation.These embodiments are only to typical case's description of the invention, however, the present invention is not limited thereto.Examination as used in the following examples
Proved recipe method is conventional method unless otherwise specified, used raw material, reagent etc., and unless otherwise specified, being can be from routine
The raw materials and reagents that the commercial sources such as commercially available obtain.
Embodiment 1
A kind of preparation method of antarctic krill oil, comprising the following steps:
(1) krill pre-processes: after the krill shrimp brick flowing water of frost is thawed, boiling enzyme deactivation is living, vacuum refrigeration
Euphausia superba powder is made in crushed after being dried；
(2) it slightly mentions: by pretreated euphausia superba powder obtained by step (1), being added 85% ethyl alcohol, 25 DEG C of Extracting temperature,
Solid-liquid ratio 1:5 (g/ml), stirring rate 800rpm, mixing time 3h are slightly mentioned mixed liquor；By gained mixed liquor with 0.22 μm
Filtering with microporous membrane processing, obtain the ethanol crude extract of antarctic krill oil；Resulting ethanol crude extract is carried out at 60 DEG C
Vacuum concentration processing, vacuum degree 0.1Mpa obtain thick shrimp sauce, yield 15.2%；
(3) it deodorant: carries out shrimp sauce prepared by step (2) to remove stench substance and dissolvent residual, vacuum degree is
0.1Mpa, temperature are room temperature, and drying time 12h obtains deodorant antarctic krill oil.
Embodiment 2
A kind of preparation method of antarctic krill oil, comprising the following steps:
(1) krill pre-processes: after the krill shrimp brick flowing water of frost is thawed, boiling enzyme deactivation is living, vacuum refrigeration
Euphausia superba powder is made in crushed after being dried；
(2) it slightly mentions: by pretreated euphausia superba powder obtained by step (1), being added 85% ethyl alcohol, 25 DEG C of Extracting temperature,
Solid-liquid ratio 1:5 (g/ml), stirring rate 800rpm, mixing time 3h are slightly mentioned mixed liquor；By gained mixed liquor with 0.22 μm
Filtering with microporous membrane processing, obtain the ethanol crude extract of antarctic krill oil；Resulting ethanol crude extract is carried out at 60 DEG C
Vacuum concentration processing, vacuum degree 0.1Mpa obtain thick shrimp sauce, yield 14.8%；
(3) refine: will in the resulting thick shrimp sauce of step (2) be added dehydrated alcohol weight it is molten, solid-liquid ratio 1:50 is vigorously stirred
30min stands 1h；To be concentrated in vacuo after processing method filtration treatment described in step (2)；It repeats the above steps 2-3 times；Through
The impurity component that the molten method of ethyl alcohol weight removes is mainly inorganic salts through detection, and it is 13.25% that imurity-removal ingredient, which accounts for thick shrimp sauce specific gravity,；
(4) it deodorant: carries out shrimp sauce prepared by step (3) to remove stench substance and dissolvent residual, vacuum degree is
0.1Mpa, temperature are room temperature, and drying time 12h obtains deodorant antarctic krill oil.
Embodiment 3
A kind of preparation method of antarctic krill oil, comprising the following steps:
(1) krill pre-processes: after the krill shrimp brick flowing water of frost is thawed, boiling enzyme deactivation is living, vacuum refrigeration
Euphausia superba powder is made in crushed after being dried；
(2) it slightly mentions: by pretreated euphausia superba powder obtained by step (1), being added 85% ethyl alcohol, 25 DEG C of Extracting temperature,
Solid-liquid ratio 1:5 (g/ml), stirring rate 800rpm, mixing time 3h are slightly mentioned mixed liquor；By gained mixed liquor with 0.22 μm
Filtering with microporous membrane processing, obtain the ethanol crude extract of antarctic krill oil；Resulting ethanol crude extract is carried out at 60 DEG C
Vacuum concentration processing, vacuum degree 0.1Mpa obtain thick shrimp sauce, yield 14.5%；
(3) refine: will in the resulting thick shrimp sauce of step (2) be added dehydrated alcohol weight it is molten, solid-liquid ratio 1:50 is vigorously stirred
30min stands 1h；To be concentrated in vacuo after processing method filtration treatment described in step (2)；It repeats the above steps 2-3 times；Through
The impurity component that the molten method of ethyl alcohol weight removes is mainly inorganic salts through detection, and it is 15% that imurity-removal ingredient, which accounts for thick shrimp sauce specific gravity,；
(4) phosphatide is enriched with: the resulting refining shrimp sauce acetone solution of step (3) is centrifuged, centrifugation rate after completely dissolution
5000rpm, time 10min；Triglyceride type antarctic krill oil is removed, enrichment shrimp sauce, yield 71.42% is made；
(5) it deodorant: carries out shrimp sauce prepared by step (4) to remove stench substance and dissolvent residual, vacuum degree is
0.1Mpa, temperature are room temperature, and drying time 12h obtains deodorant antarctic krill oil.
Embodiment 4
A kind of preparation method of antarctic krill oil, comprising the following steps:
(1) krill pre-processes: after the krill shrimp brick flowing water of frost is thawed, boiling enzyme deactivation is living, vacuum refrigeration
Euphausia superba powder is made in crushed after being dried；
(2) it slightly mentions: by pretreated euphausia superba powder obtained by step (1), being added dehydrated alcohol, 30 DEG C of Extracting temperature,
Solid-liquid ratio 1:10 (g/ml), stirring rate 800rpm, mixing time 1h are slightly mentioned mixed liquor；By gained mixed liquor with 0.22 μ
The filtering with microporous membrane of m is handled, and obtains the ethanol crude extract of antarctic krill oil；Resulting ethanol crude extract is carried out at 35 DEG C
Vacuum concentration processing, vacuum degree 0.095Mpa obtain thick shrimp sauce, yield 8.19%；
(3) refine: will in the resulting thick shrimp sauce of step (2) be added n-hexane weight it is molten, solid-liquid ratio 1:30 is vigorously stirred
30min stands 1h；To be concentrated in vacuo after processing method filtration treatment described in step (2)；It repeats the above steps 2-3 times；Through
The impurity component that the molten method of n-hexane weight removes is mainly protein through detection, and it is 6.7% that imurity-removal ingredient, which accounts for thick shrimp sauce specific gravity,；
(4) phosphatide is enriched with: the resulting refining shrimp sauce acetone solution of step (3) is centrifuged, centrifugation rate after completely dissolution
6000rpm, time 10min；Triglyceride type antarctic krill oil is removed, enrichment shrimp sauce, yield 70.03% is made；
(5) it deodorant: carries out shrimp sauce prepared by step (4) to remove stench substance and dissolvent residual, vacuum degree is
0.1Mpa, temperature are set as 60 DEG C, and drying time 2h obtains deodorant antarctic krill oil.
Embodiment 5
A kind of preparation method of antarctic krill oil, comprising the following steps:
(1) krill pre-processes: after the krill shrimp brick flowing water of frost is thawed, boiling enzyme deactivation is living, vacuum refrigeration
Euphausia superba powder is made in crushed after being dried；
(2) it slightly mentions: by pretreated euphausia superba powder obtained by step (1), being added 95% ethyl alcohol, 25 DEG C of Extracting temperature,
Solid-liquid ratio 1:10 (g/ml), stirring rate 800rpm, mixing time 2h are slightly mentioned mixed liquor；By gained mixed liquor with 0.22 μ
The filtering with microporous membrane of m is handled, and obtains the ethanol crude extract of antarctic krill oil；By resulting ethyl alcohol mixed extract at 40 DEG C
Carry out vacuum concentration processing, vacuum degree 0.1Mpa.Obtain thick shrimp sauce, yield 14.05%；
(3) refine: will in the resulting thick shrimp sauce of step (2) be added dehydrated alcohol weight it is molten, solid-liquid ratio 1:20 is vigorously stirred
30min stands 1h；To be concentrated in vacuo after processing method filtration treatment described in step (2)；It repeats the above steps 2-3 times；Through
The impurity component that the molten method of ethyl alcohol weight removes is mainly inorganic salts through detection, and it is 10.81% that imurity-removal ingredient, which accounts for thick shrimp sauce specific gravity,；
(4) phosphatide is enriched with: step (3) 30% ethyl alcohol of resulting refining shrimp sauce is dissolved, is centrifuged after completely dissolution, from
The heart rate 5000rpm, time 10min；The triglyceride type antarctic krill oil for removing the oily that mixing liquid level is precipitated, is made enrichment
Shrimp sauce, yield 88.3%；
(5) it deodorant: carries out shrimp sauce prepared by step (4) to remove stench substance and dissolvent residual, vacuum degree is
0.1Mpa, temperature are set as room temperature, are dried overnight, and obtain deodorant antarctic krill oil.
Embodiment 6
The difference of the present embodiment and embodiment 3 is that centrifugation rate is 1000rpm in step (4).
Embodiment 7
The difference of the present embodiment and embodiment 5 is, 30% ethyl alcohol in step (4) is changed to acetone.
Comparative example 1
The difference of this comparative example and embodiment 3 is, does not include step (5).
Comparative example 2
The difference of this comparative example and embodiment 3 is that the temperature of step (5) is 70 DEG C.
Test example
For embodiment 1-7 and comparative example 1-2, the sense organ of final products is evaluated, and detected respectively
It refines shrimp sauce, be enriched with EPA, DHA and content of phospholipid in shrimp sauce and final products, as a result as shown in table 1,2.
(1) sensory evaluation: evaluating product color and taste, and weighs after defishying compared with shrimp sauce before defishying
Mass lost percentage (removing volatile component)；
(2) EPA, DHA: EPA, DHA mass account for the percentage of shrimp sauce quality in shrimp sauce；
Content of phospholipid: it is scaled every g shrimp sauce how many quality (mg) phosphatide.
1 final products sensory evaluation of table
| Color | Taste | Remove volatile component | |
| Embodiment 1 | Color is muddy | Without bad smell | 0.83% |
| Embodiment 2 | It is limpid in sight | Without bad smell | 0.86% |
| Embodiment 3 | It is limpid in sight | Without bad smell | 0.85% |
| Embodiment 4 | It is limpid in sight | Substantially without bad smell | 1.96% |
| Embodiment 5 | It is limpid in sight | Without bad smell | 0.7% |
| Embodiment 6 | It is limpid in sight | Without bad smell | 0.8% |
| Embodiment 7 | It is limpid in sight | Without bad smell | 0.72% |
| Comparative example 1 | It is limpid in sight | There is denseer bad smell | - |
| Comparative example 2 | It is limpid in sight | There is bad smell | 2.3% |
Table 2 refines shrimp sauce, is enriched with EPA, DHA and content of phospholipid in shrimp sauce and final products
It can be seen from table 1,2 present invention by vacuum drying defishying, can effectively remove krill oil bad smell and
Dissolvent residual, shrimp sauce is substantially without bad smell on sense organ.And the shrimp sauce bad smell of non-defishying is obvious.2 temperature of comparative example is excessively high,
It easily causes certain ingredient oxygenolysis in shrimp sauce and generates new stench substance, bad smell after vacuum deodorant is caused not drop to ideal
Range.
4 deodorant temperature of embodiment is higher, and antarctic krill oil removes volatile component 1.96%, the bad smell of shrimp sauce on sense organ
It is thin, the slightly above deodorant krill oil of other embodiments, this is because temperature height removing volatile materials can be high, but the high shrimp of temperature
Oil itself can lead to the new stench substance generated because temperature high oxidation is rotten, and therefore, deodorant temperature and time, which should control, to be closed
It manages in range.
Embodiment 2-7 is limpider compared with the color of 1 shrimp sauce of embodiment, this is because embodiment 2-7 is carried out after slightly mentioning
Refining, refining can effectively remove the impurity such as protein, inorganic salts, color made to become to clarify.
Embodiment 3 is higher compared with the content of phospholipid and EPA, DHA content of embodiment 1-2 final products, this is because passing through
Phosphatide enrichment process can significantly improve content of phospholipid.
Embodiment 3 is more preferable compared with 6 concentration effect of embodiment, it can thus be seen that improving centrifugal rotational speed, can obtain preferably
Concentration effect.
Embodiment 7 is more preferable compared with 5 phosphatide concentration effect of embodiment, it is seen then that is enriched with, can be obtained preferably using acetone
Concentration effect.
And the entire technique of embodiment 5 only with ethyl alcohol as solvent, it is more environmentally-friendly, and the content of phospholipid of final products
It is higher, realize a kind of integral manufacturing technique of extraction, refining and enrichment that antarctic krill oil is only carried out using ethyl alcohol.
Although illustrate and describing the present invention with specific embodiment, it will be appreciated that without departing substantially from of the invention
Many other change and modification can be made in the case where spirit and scope.It is, therefore, intended that wrapping in the following claims
Include all such changes and modifications belonged in the scope of the invention.
Claims (10)
1. a kind of preparation method of antarctic krill oil, which comprises the following steps:
The antarctic krill oil that krill is obtained after extracted carries out defishying, obtains deodorant antarctic krill oil；
Wherein, defishying includes: dry 1-24h under the conditions of vacuum degree 0.02-0.15MPa, 20-60 DEG C；It includes thick for extracting
It mentions and optional refining.
2. the preparation method of antarctic krill oil described in accordance with the claim 1, which is characterized in that the vacuum degree of defishying is
0.05-0.1MPa, drying temperature are 20-30 DEG C, drying time 12-24h.
3. the preparation method of antarctic krill oil described in accordance with the claim 1, which is characterized in that slightly mention the following steps are included:
Coarse extraction and separation are carried out to krill with thick extraction solvent, obtain crude extract, crude extract is concentrated, obtains thick shrimp sauce；
Preferably, thick extraction solvent include one of the alcohols of C1-C4, the ketone of C1-C4, the alkanes of C1-C6 or No. 6 gasoline or
Several, preferably one or more of the alcohols of C1-C4 or the alkanes of C1-C6, preferably ethyl alcohol or n-hexane are further excellent
It is selected as ethyl alcohol；
Preferably, the water content of ethyl alcohol is 0-20%；
Preferably, it is 20-30 DEG C, extraction time 1-3h, solid-liquid ratio 1:5- that the process conditions of coarse extraction, which include: Extracting temperature,
1:10g/mL；
Preferably, it is concentrated as vacuum concentration；Preferably, it is 35-80 DEG C that the process conditions of vacuum concentration, which include: thickening temperature, very
Reciprocal of duty cycle is 0.09-0.1MPa.
4. the preparation method of antarctic krill oil described in accordance with the claim 1, which is characterized in that refining the following steps are included:
It repeats step (I) 2-3 times, obtains refining shrimp sauce；Step (I) includes: to be carried out with refining solvent to the thick shrimp sauce after slightly mentioning
Weight is molten and separates, and obtains refining liquid, then refining liquid is concentrated；
Preferably, refining solvent include one of the alcohols of C1-C4, the ketone of C1-C4, the alkanes of C1-C6 or No. 6 gasoline or
Several, preferably one or more of the alcohols of C1-C4 or the alkanes of C1-C6, preferably ethyl alcohol or n-hexane are further excellent
It is selected as dehydrated alcohol；
Preferably, weight is molten is carried out in the case where strength mixes, and the molten process conditions of weight include: that incorporation time is 20-40min, material
Liquor ratio is 1:20-1:50g/mL；
Preferably, 1-2h, then isolated refining liquid are stood after weight is molten；
Preferably, it is concentrated as vacuum concentration；Preferably, it is 35-80 DEG C that the process conditions of vacuum concentration, which include: thickening temperature, very
Reciprocal of duty cycle is 0.09-0.1MPa.
5. according to the preparation method of the described in any item antarctic krill oils of claim 1-4, which is characterized in that the krill
For pretreated euphausia superba powder；
Preferably, pretreatment the following steps are included:
After the krill shrimp brick of frost is thawed, boiling enzyme deactivation is living, crushes after vacuum freeze drying, obtains euphausia superba powder.
6. according to the preparation method of the described in any item antarctic krill oils of claim 1-4, which is characterized in that the method is also wrapped
Include: the antarctic krill oil that krill is obtained after extracted first carries out the step of phosphatide enrichment carries out defishying again；
Preferably, phosphatide enrichment the following steps are included:
The krill after extraction is sufficiently dissolved and separated with solvent-rich, obtains enrichment shrimp sauce；
Preferably, solvent-rich include one of the alcohols of C1-C4, the ketone of C1-C4, the alkanes of C1-C6 or No. 6 gasoline or
It is several, preferably one or more of the alcohols of C1-C4 or the ketone of C1-C4, preferably ethyl alcohol or acetone, further preferably
For ethyl alcohol；
Preferably, separate mode is centrifugation；Preferably, centrifugation rate 4000-6000rpm, centrifugation time 10-15min.
7. according to the preparation method of the described in any item antarctic krill oils of claim 1-4, which is characterized in that including following step
It is rapid:
(a) after the krill shrimp brick of frost thawing, boiling enzyme deactivation is living, crushes after vacuum freeze drying, obtains krill
Powder；
(b) coarse extraction and separation are carried out to euphausia superba powder with the ethyl alcohol of water content 0-20%, obtains crude extract, crude extract is through true
Sky concentration, obtains thick shrimp sauce；
The process conditions of coarse extraction include: that Extracting temperature is 20-30 DEG C, extraction time 1-3h, solid-liquid ratio 1:5-1:10g/
mL；
The process conditions of vacuum concentration include: that thickening temperature is 35-80 DEG C, vacuum degree 0.09-0.1MPa；
(c) molten, the solid-liquid ratio 1:20-1:50g/mL of weight, incorporation time are carried out in the case where strength mixes to thick shrimp sauce with dehydrated alcohol
For 20-40min, 1-2h, then isolated refining liquid are stood after weight is molten；Refining liquid is concentrated in vacuo, the work of vacuum concentration
Skill condition includes: that thickening temperature is 35-80 DEG C, vacuum degree 0.09-0.1MPa；
(d) it repeats step (c) 2-3 times, obtains refining shrimp sauce；
(e) refining shrimp sauce is dissolved with ethyl alcohol, is centrifuged after completely dissolution, centrifugation rate 4000-6000rpm, when centrifugation
Between 10-15min, obtain enrichment shrimp sauce；
(f) it will be enriched with shrimp sauce dry 2-24h under the conditions of vacuum degree 0.05-0.1MPa, 25-60 DEG C, obtain deodorant krill
Oil.
8. a kind of deodorant antarctic krill oil, which is characterized in that using the system of the described in any item antarctic krill oils of claim 1-7
Preparation Method is prepared.
9. a kind of deodorant antarctic krill oil according to any one of claims 8 is preparing the application in food.
10. a kind of food, which is characterized in that be mainly prepared by deodorant antarctic krill oil according to any one of claims 8.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201811116201.2A CN109207247A (en) | 2018-09-25 | 2018-09-25 | The preparation method of antarctic krill oil, deodorant antarctic krill oil and its application, food |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201811116201.2A CN109207247A (en) | 2018-09-25 | 2018-09-25 | The preparation method of antarctic krill oil, deodorant antarctic krill oil and its application, food |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN109207247A true CN109207247A (en) | 2019-01-15 |
Family
ID=64984816
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201811116201.2A Pending CN109207247A (en) | 2018-09-25 | 2018-09-25 | The preparation method of antarctic krill oil, deodorant antarctic krill oil and its application, food |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN109207247A (en) |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110878231A (en) * | 2019-06-25 | 2020-03-13 | 山东鲁华海洋生物科技有限公司 | Low-arsenic euphausia superba oil and preparation method thereof |
| CN115109649A (en) * | 2022-08-29 | 2022-09-27 | 逢时(青岛)海洋科技有限公司 | Euphausia superba oil refining process |
Citations (16)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102041166A (en) * | 2011-01-20 | 2011-05-04 | 山东师范大学 | Method for extracting krill oil with high phosphatide content from Antarctic krills |
| CN102224855A (en) * | 2011-05-13 | 2011-10-26 | 中国海洋大学 | Method for enriching lipid in Antarctic krill |
| CN102585995A (en) * | 2012-02-21 | 2012-07-18 | 山东师范大学 | Method for preparing shrimp sauce of antarctic krill with high phospholipid content |
| CN102585994A (en) * | 2012-02-21 | 2012-07-18 | 山东师范大学 | Method for preparing Antarctic krill shrimp sauce with high content of phospholipid |
| CN102925284A (en) * | 2012-10-25 | 2013-02-13 | 山东师范大学 | Technique for extracting low-cholesterol-content krill oil from Antarctic krill |
| CN103320217A (en) * | 2013-07-11 | 2013-09-25 | 日照海大博远海洋生物科技有限公司 | Method for extracting krill oil rich in phospholipid from euphausia superba |
| US20140065236A1 (en) * | 2009-10-30 | 2014-03-06 | Tharos Ltd. | Solvent-free process for obtaining phospholipids and neutral enriched krill oils |
| CN103981021A (en) * | 2014-05-31 | 2014-08-13 | 山东乾清翔泰生物制品有限公司 | Method for refining krill oil from Antarctic krill powder |
| CN104479850A (en) * | 2014-12-15 | 2015-04-01 | 中国水产科学研究院黄海水产研究所 | Method for extracting krill oil with high phospholipid content from fresh Antarctic krill |
| CN104498180A (en) * | 2014-12-20 | 2015-04-08 | 中国水产科学研究院黄海水产研究所 | Method for extracting high-purity phospholipid from Antarctic krill oil |
| CN105536275A (en) * | 2016-01-29 | 2016-05-04 | 山东鲁华海洋生物科技有限公司 | Method and device for concentrating and separating euphausia superb oil extraction liquid |
| US20170313956A1 (en) * | 2014-11-14 | 2017-11-02 | Tharos Ltd. | Solvent-Free Process for Obtaining Phospholipids and Neutral Enriched Krill Oils Using Melting and Evaporation |
| CN207243851U (en) * | 2017-08-22 | 2018-04-17 | 青岛南极维康生物科技有限公司 | A kind of antarctic krill oil extraction system |
| CN108004016A (en) * | 2017-12-18 | 2018-05-08 | 青岛康境海洋生物科技有限公司 | A kind of preparation method of the antarctic krill oil of high lecithin content |
| CN108179053A (en) * | 2018-01-26 | 2018-06-19 | 日照职业技术学院 | A kind of preparation method of high quality Antarctic krill oil |
| CN108329988A (en) * | 2018-02-02 | 2018-07-27 | 中国水产科学研究院黄海水产研究所 | Method for extracting krill oil from Antarctic krill |
-
2018
- 2018-09-25 CN CN201811116201.2A patent/CN109207247A/en active Pending
Patent Citations (16)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20140065236A1 (en) * | 2009-10-30 | 2014-03-06 | Tharos Ltd. | Solvent-free process for obtaining phospholipids and neutral enriched krill oils |
| CN102041166A (en) * | 2011-01-20 | 2011-05-04 | 山东师范大学 | Method for extracting krill oil with high phosphatide content from Antarctic krills |
| CN102224855A (en) * | 2011-05-13 | 2011-10-26 | 中国海洋大学 | Method for enriching lipid in Antarctic krill |
| CN102585995A (en) * | 2012-02-21 | 2012-07-18 | 山东师范大学 | Method for preparing shrimp sauce of antarctic krill with high phospholipid content |
| CN102585994A (en) * | 2012-02-21 | 2012-07-18 | 山东师范大学 | Method for preparing Antarctic krill shrimp sauce with high content of phospholipid |
| CN102925284A (en) * | 2012-10-25 | 2013-02-13 | 山东师范大学 | Technique for extracting low-cholesterol-content krill oil from Antarctic krill |
| CN103320217A (en) * | 2013-07-11 | 2013-09-25 | 日照海大博远海洋生物科技有限公司 | Method for extracting krill oil rich in phospholipid from euphausia superba |
| CN103981021A (en) * | 2014-05-31 | 2014-08-13 | 山东乾清翔泰生物制品有限公司 | Method for refining krill oil from Antarctic krill powder |
| US20170313956A1 (en) * | 2014-11-14 | 2017-11-02 | Tharos Ltd. | Solvent-Free Process for Obtaining Phospholipids and Neutral Enriched Krill Oils Using Melting and Evaporation |
| CN104479850A (en) * | 2014-12-15 | 2015-04-01 | 中国水产科学研究院黄海水产研究所 | Method for extracting krill oil with high phospholipid content from fresh Antarctic krill |
| CN104498180A (en) * | 2014-12-20 | 2015-04-08 | 中国水产科学研究院黄海水产研究所 | Method for extracting high-purity phospholipid from Antarctic krill oil |
| CN105536275A (en) * | 2016-01-29 | 2016-05-04 | 山东鲁华海洋生物科技有限公司 | Method and device for concentrating and separating euphausia superb oil extraction liquid |
| CN207243851U (en) * | 2017-08-22 | 2018-04-17 | 青岛南极维康生物科技有限公司 | A kind of antarctic krill oil extraction system |
| CN108004016A (en) * | 2017-12-18 | 2018-05-08 | 青岛康境海洋生物科技有限公司 | A kind of preparation method of the antarctic krill oil of high lecithin content |
| CN108179053A (en) * | 2018-01-26 | 2018-06-19 | 日照职业技术学院 | A kind of preparation method of high quality Antarctic krill oil |
| CN108329988A (en) * | 2018-02-02 | 2018-07-27 | 中国水产科学研究院黄海水产研究所 | Method for extracting krill oil from Antarctic krill |
Non-Patent Citations (1)
| Title |
|---|
| 徐文思: "南极磷虾油的提取及精制工艺研究", 《中国优秀硕士学位论文全文数据库》 * |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110878231A (en) * | 2019-06-25 | 2020-03-13 | 山东鲁华海洋生物科技有限公司 | Low-arsenic euphausia superba oil and preparation method thereof |
| CN115109649A (en) * | 2022-08-29 | 2022-09-27 | 逢时(青岛)海洋科技有限公司 | Euphausia superba oil refining process |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CA2115571A1 (en) | Process for extracting lipids with a high proportion of long chain, highly unsaturated fatty acids | |
| CN102041166B (en) | Method for extracting krill oil with high phosphatide content from Antarctic krills | |
| FI120648B (en) | Process for the preparation of arachidonic acid | |
| AU2012242355B2 (en) | A process for the isolation of a phospholipid | |
| CN104140880B (en) | A kind of preparation method of crocodile essential oil | |
| KR101507820B1 (en) | Method for manufacturing soy sauce by using of Dendropanax morbifera material fermentation | |
| CN102143690A (en) | Oil containing one or more long-chain polyunsaturated fatty acids phospholipids derived from biomass | |
| CN111406110A (en) | Preparation of algae polyunsaturated fatty acids | |
| KR101855798B1 (en) | Method of preparing soybean sauce using guamegi and jeju mandarin | |
| CN107858197A (en) | A kind of production and processing method of low acid value high-quality edible oil tea-seed oil | |
| CN109207247A (en) | The preparation method of antarctic krill oil, deodorant antarctic krill oil and its application, food | |
| CN104962589A (en) | Microbial oil rich in phospholipid type polyunsaturated fatty acid and preparation method thereof | |
| CN109463605A (en) | A kind of antarctic krill oil solid beverage and preparation method thereof | |
| CN104046511B (en) | Improve the linseed oil preparation method of linseed oil quality | |
| CN109971538A (en) | A method of egg oil is prepared using Propane abstraction technique | |
| JP2017122067A (en) | Oil of euphausia pacifica and method for producing the same | |
| CN102771582A (en) | Edible blend oil added with euphausia superba oil | |
| CN101731347B (en) | Curcumin-containing liquid dairy product and preparation method thereof | |
| CN110257155A (en) | The sub-critical extraction method and gained sea cucumber intestine egg oil of sea cucumber intestine egg oil | |
| CN104938652A (en) | Preparation method of blended edible oil of peony seed oil and walnut oil | |
| Rizvi et al. | Extraction of oil from flaxseed using three phase partitioning | |
| CN107586608A (en) | A kind of method of vegetable oil degumming | |
| US20080268117A1 (en) | Method of purifying oils containing epa and dha | |
| CN102731359B (en) | Extraction method of astaxanthin and ester thereof from antarctic krill | |
| WO2010087373A1 (en) | Method for producing extract of astaxanthin-containing yeast belonging to genus phaffia |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| CB03 | Change of inventor or designer information | ||
| CB03 | Change of inventor or designer information |
Inventor after: Pan Haobo Inventor after: Zhang Xiaohui Inventor after: Wang Jianyun Inventor after: Yin Jia Inventor after: Zhou Xin Inventor after: Zhong Zhou Inventor before: Zhang Xiaohui Inventor before: Wang Jianyun Inventor before: Yin Jia Inventor before: Zhou Xin Inventor before: Zhong Zhou |
|
| RJ01 | Rejection of invention patent application after publication | ||
| RJ01 | Rejection of invention patent application after publication |
Application publication date: 20190115 |