CN109197543A - A kind of acclimation method of Plantlets of Strawberry - Google Patents
A kind of acclimation method of Plantlets of Strawberry Download PDFInfo
- Publication number
- CN109197543A CN109197543A CN201811135977.9A CN201811135977A CN109197543A CN 109197543 A CN109197543 A CN 109197543A CN 201811135977 A CN201811135977 A CN 201811135977A CN 109197543 A CN109197543 A CN 109197543A
- Authority
- CN
- China
- Prior art keywords
- strawberry
- domestication
- plantlets
- seedling
- tissue
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G31/00—Soilless cultivation, e.g. hydroponics
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G24/00—Growth substrates; Culture media; Apparatus or methods therefor
- A01G24/10—Growth substrates; Culture media; Apparatus or methods therefor based on or containing inorganic material
- A01G24/12—Growth substrates; Culture media; Apparatus or methods therefor based on or containing inorganic material containing soil minerals
- A01G24/15—Calcined rock, e.g. perlite, vermiculite or clay aggregates
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G24/00—Growth substrates; Culture media; Apparatus or methods therefor
- A01G24/20—Growth substrates; Culture media; Apparatus or methods therefor based on or containing natural organic material
- A01G24/28—Growth substrates; Culture media; Apparatus or methods therefor based on or containing natural organic material containing peat, moss or sphagnum
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Environmental Sciences (AREA)
- Soil Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Inorganic Chemistry (AREA)
- Cultivation Of Plants (AREA)
Abstract
The invention discloses a kind of acclimation methods of Plantlets of Strawberry, domestication of uncapping, greenhouse including tissue-cultured seedling tame again, washes root, cultivation and management, tissue-cultured seedling is tamed twice before transplanting, improve its adaptability, and synthesis provides the cultivation condition of domestication preferably, acclimation method through the invention, strawberry domestication high survival rate, seedling-slowing stage is short, growth is fast, can be effectively improved strawberry quality；And the method for the present invention is simple, easily grasps；Construction domestication place is simple, and cost is relatively low, and can reuse, if domestication terminates, greenhouse, which also can be carried out, plants other crops.
Description
Technical field
The invention belongs to garden crop nursery stock method for culturing seedlings, and in particular to the method for Plantlets of Strawberry domestication.
Background technique
Strawberry is rosaceae Fragaria herbaceos perennial, belongs to one of big fruit in the world today seven, is known as " fruit
Queen ".There are cultivation in all parts of the country, and red-color strawberries are the main breeds of Jiangsu Province at present, and red-color strawberries plant growing way is strong, fruit
It is larger, pol is high, hardness is big, shelf-stable.Therefore it is well received by consumers, but natural propagation rate is very low.Since production is sent out
Rapidly, the seedling demand of strawberry is very big for exhibition.Traditionally, the seeling industry of strawberry uses crawl stem breeding and division propagation, but long
Phase can make strawberry virus infection using this asexual reproduction method, accumulate and make variety deterioration.Radical cure strawberry is not yet found at present
The active drug of virosis.The fast numerous production of strawberry detoxic seedling may be implemented using tissue culture technique in recent years.But to grass
The report of certain kind of berries tissue culture transplantation of seedlings acclimation conditions research is less.Especially in the main cultivation area in south and subtropical zone, strawberry nursery is general
In 3 one April in spring.This just needs to tame and docile the tissue-cultured seedling of indoor culture in outdoor carry out transition in the summer and autumn of the previous year
Change, by the cultivating and managing in winter, the female seedling for being sufficiently used for breeding is provided for the coming year.And when the summer and autumn of south China plains region
Section, weather are more hot.Strawberry seedlings optimum growth temperature is 20 1 26 DEG C.In the southern torrid zone and subtropical zone area, due to summer
Temperature is higher, and intensity of illumination is big, test tube seedling domestication must be carried out under the conditions of particular device, field nature transition domestication substantially without
Method survival.Therefore, it inquires into Plantlets of Strawberry and tames transition method in summer, establish the skill that Plantlets of Strawberry is domesticated for field mother's seedling
Art system also seems very necessary.
Summary of the invention
Goal of the invention: aiming at the problem that strawberry field mother shoot survival percent in the prior art, the present invention provides one kind to help
In establish subtropical zone summer carry out simple and efficient Plantlets of Strawberry acclimation method.
A kind of technical solution: acclimation method of Plantlets of Strawberry of the present invention, comprising the following steps:
(1) uncap domestication: selection has 4-5 piece leaf, and height of seedling is not less than 3cm, the tissue culture seedling of well developed root system, in laboratory
It uncaps and tames 2-6d；
(2) it tames again: the tissue culture bottle seedling after domestication of uncapping being transferred in greenhouse and is tamed 1-3d again；
(3) it washes root: carrying out the tissue-cultured seedling in bottle after taming again to wash root, old root is trimmed when washing root and protects tender, stimulates root
Growth；
(4) it cultivates: cleaned tissue-cultured seedling being cultivated in matrix hole tray after sterilization, the tissue-cultured seedling hole tray that will have been cultivated
It is placed on the chiltern seedbed sprinkled profoundly water, Small plastic shed is added above hole tray and is covered with plastic film and sunshade net, the substrate composition
For vermiculite: perlite: turf=1:1:3-6；
(5) it manages: sunshade net being opened into ventilation sooner or later, is gradually divulged information after 3-5d, if here discovery Strawberry Seedlings wither, pour in time
Water and lid sunshade net；Sunshade net and plastic film are all removed after 10-15d, survival rate after statistics strawberry domestication.
In step (2), the acclimation conditions again in the greenhouse are temperature at 25-30 DEG C, humidity 75%-90%, illumination
Intensity is exactly that the illumination of normal daytime does not need artificial light filling.
In step (3), culture medium has to clean up when washing root, otherwise causes the raw bacterium infection in root, will have a direct impact on
The survival rate of detoxic seedling.
In step (4), the matrix is carried out using 800-1000 times of 85%-90% carbendazol wettable powder solution
Disinfection.
In step (4), the substrate composition is vermiculite: perlite: turf=1:1:3.
In step (5), sunshade net is opened into the 1-2h that divulges information sooner or later, divulge information 4-6h, illumination 4-7h daily after 3-5d；It was found that
Here Strawberry Seedlings wither, in time watering and lid sunshade net, keep water content of substrate 80% -90%.
Acclimation method of the present invention is tamed twice using laboratory and greenhouse, can be significantly improved Plantlets of Strawberry and be entered field
Survival rate.
The utility model has the advantages that method of the invention is simple, easily grasps；Strawberry domestication high survival rate, seedling-slowing stage is short, growth is fast, energy
Enough it is effectively improved strawberry quality；Construction domestication place is simple, and cost is relatively low, and can reuse, if domestication terminates, greatly
Canopy, which also can be carried out, plants other crops.
Specific embodiment
The application is explained in detail combined with specific embodiments below.
A kind of method for improving Plantlets of Strawberry domestication of embodiment (by taking beauty as an example)
1, uncap domestication: selection has 4-5 piece leaf, and plant height is no more than the Plantlets of Strawberry of 3cm, uncaps domestication in laboratory
The tissue-cultured seedling uncapped then is transferred to greenhouse and tames 1d again by 3d.
2, prepare matrix: matrix (vermiculite: perlite: turf) is fitted into 50 hole hole trays and is sprinkled profoundly water by transplanting the previous day,
Each embodiment does 2 kinds of processing, and a kind of 85% carbendazol wettable powder solution with 800 times carries out disinfection processing, another
It is not processed.Each embodiment handles 20 hole trays.
1 embodiment substrate composition of table
3, it washes root: the tissue-cultured seedling in bottle is taken out to the culture medium for cleaning root with flowing water, old root protection is trimmed in cleaning
Tender, stimulate the growth of root.Otherwise culture medium, which has to clean up, causes the raw bacterium infection in root, influence surviving for detoxic seedling
Rate.
4, it transplants: after cleaning up, by tissue culture transplantation of seedlings to the hole tray for installing matrix, being watered with root water, be placed on big
On the chiltern seedbed sprinkled profoundly water in canopy.Chiltern seedbed is mainly hole tray moisturizing, and the humidity for extending hole tray improves strawberry domestication
Survival rate.
5, plus arched shed covers plastic film: taking arched shed above hole tray and covers plastic film and cover sunshade net.
6, manage after being colonized: sunshade net is taken away sooner or later daily, is gradually divulged information after 3d by preceding 3d after field planting, if discovery grass
Here certain kind of berries seedling withers, and moisturizing is built in watering in time.Plastic film and sunshade net are all taken away in 10d, count survival rate.10d
30d is cultivated in normal seedling management afterwards, investigates the upgrowth situation of red-color strawberries tissue-cultured seedling.
2 30d Strawberry Plantlets shoot survival percent of table and upgrowth situation statistics
The matrix of embodiment 1 (vermiculite: perlite: turf=1:1:3) by disinfection treatment is found from above-described embodiment
Strawberry Plantlets shoot survival percent highest, up to 98%.Embodiment 2 (vermiculite: perlite: turf=1:1:1) by disinfection and not
The matrix Strawberry Plantlets shoot survival percent of disinfection treatment is all very low, is primarily due to vermiculite, perlite and turf proportion and is not suitable for
The growth of Plantlets of Strawberry, 2 matrix of embodiment is loose, water retention property is bad.The newborn number of sheets is few, and petiole elongation is slow, and dry matter contains
Measure it is low, plant stretch 6.6cm, it is shorter.Secondly base of the embodiment 3 (vermiculite: perlite: turf=1:1:2) by disinfection treatment
Matter Strawberry Plantlets shoot survival percent 96.4%, does not disinfect Strawberry Plantlets shoot survival percent 79.6%.(the vermiculite: pearl of embodiment 4
Rock: turf=2:1:3) pass through disinfection is 78.8% He respectively with the matrix Strawberry Plantlets shoot survival percent that do not disinfect
73.6%.The newborn number of sheets is 3.7 and 3.9 respectively, and petiole length is 3.6 and 3.7cm respectively, and plant stretches 7.3cm and 7.1cm.It is dry
Content of material is 10.36 and 9.87 respectively, and the proportion (vermiculite: perlite: turf=1:1:3) and process of 1 matrix of embodiment disappear
The matrix of poison processing is suitable for the domestication of Plantlets of Strawberry, and Plantlets of Strawberry high survival rate, the newborn number of sheets is most, and dry matter content is most
Greatly, plant stretches longest.
Comparative example 1
Matrix disinfection is irrigated with 50% 1000 times of liquid of wettable powder of carbendazim, remaining operation is with embodiment 1, through comparing
It was found that 1 Strawberry Plantlets shoot survival percent of comparative example only has 62%, it is far below embodiment 1, and tissue-cultured seedling survives the rear newborn number of sheets, leaf
Handle is long, plant stretches and dry weight is below embodiment 1, and statistics is shown in Table 3.
Survival rate and upgrowth situation statistics after 3 comparative example 1 of table and the domestication of 1 Plantlets of Strawberry of embodiment
Comparative example 2
It is directly cultivated without domestication, remaining operation is with embodiment 1, it was found that the Plantlets of Strawberry without domestication is direct
It is very high to cultivate the death rate, the environment of this field mutability may be directly cultivated from more stable laboratory environment, tissue-cultured seedling is suitable
It answers external environment ability poor, causes most Strawberry Seedlings dead, only small part survives, and statistical result is shown in Table 4.
Survival rate and upgrowth situation statistics after 4 comparative example 2 of table and the domestication of 1 Plantlets of Strawberry of embodiment
Comparative example 3
Laboratory Acclimation is only carried out, greenhouse domestication is cancelled, remaining operation is with embodiment 1, it was found that comparative example 3 processes
Laboratory Acclimation tissue culture shoot survival percent can satisfy tissue-cultured seedling cultivation in the requirement in field, but its upgrowth situation is low 82%
In embodiment 1, statistical result is shown in Table 5.
Survival rate and upgrowth situation statistics after 5 comparative example 3 of table and the domestication of 1 Plantlets of Strawberry of embodiment
Comparative example 4
Greenhouse domestication is only carried out, Laboratory Acclimation is cancelled, remaining operation is with embodiment 1, and warp is it was found that comparative example 4 warps
It crosses greenhouse and tames its survival rate 78%, survival rate is lower compared with Example 1, does not meet cultivation condition, both wastes labour
The waste of resource is caused simultaneously, embodiment 1 is optimal scheme in summary, Plantlets of Strawberry high survival rate after taming, and
Growing way is good.Statistical result is shown in Table 6.
Survival rate and upgrowth situation statistics after 6 comparative example 4 of table and the domestication of 1 Plantlets of Strawberry of embodiment
Claims (5)
1. a kind of acclimation method of Plantlets of Strawberry, which comprises the following steps:
(1) uncap domestication: selection has 4-5 piece leaf, and height of seedling is not less than 3cm, and the tissue culture seedling of well developed root system is uncapped in laboratory
Tame 2-6d；
(2) it tames again: the tissue culture bottle seedling after domestication of uncapping being transferred in greenhouse and is tamed 1-3d again；
(3) it washes root: carrying out the tissue-cultured seedling in bottle after taming again to wash root, old root is trimmed when washing root and protects tender；
(4) it cultivates: cleaned tissue-cultured seedling being cultivated in matrix hole tray after sterilization, the tissue-cultured seedling hole tray cultivated is placed on
On the chiltern seedbed sprinkled profoundly water, Small plastic shed is added above hole tray and is covered with plastic film and sunshade net, the substrate composition is leech
Stone: perlite: turf=1:1:3-6；
(5) it manages: sunshade net being opened into ventilation sooner or later, is gradually divulged information after 3d-5d, if here discovery Strawberry Seedlings wither, watering in time is simultaneously
Lid sunshade net；Sunshade net and plastic film are all removed after 10-15d, survival rate after statistics strawberry domestication.
2. the acclimation method of Plantlets of Strawberry according to claim 1, which is characterized in that in step (2), in the greenhouse
Acclimation temperature again in 25-30 DEG C, humidity 75%-90%.
3. the acclimation method of Plantlets of Strawberry according to claim 1, which is characterized in that in step (4), the matrix is adopted
It is carried out disinfection with 800-1000 times of 85%-90% carbendazol wettable powder solution.
4. the acclimation method of Plantlets of Strawberry according to claim 1, which is characterized in that in step (4), the matrix is matched
Than for vermiculite: perlite: turf=1:1:3.
5. the acclimation method of Plantlets of Strawberry according to claim 1, which is characterized in that in step (5), sooner or later by sunshade
Net opens the 1-2h that divulges information, and divulge information 4-6h, illumination 4-7h daily after 3-5d, while keeping water content of substrate 80% -90%.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201811135977.9A CN109197543A (en) | 2018-09-28 | 2018-09-28 | A kind of acclimation method of Plantlets of Strawberry |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201811135977.9A CN109197543A (en) | 2018-09-28 | 2018-09-28 | A kind of acclimation method of Plantlets of Strawberry |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN109197543A true CN109197543A (en) | 2019-01-15 |
Family
ID=64981958
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201811135977.9A Pending CN109197543A (en) | 2018-09-28 | 2018-09-28 | A kind of acclimation method of Plantlets of Strawberry |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN109197543A (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111109060A (en) * | 2019-11-29 | 2020-05-08 | 南京农业大学 | Method for increasing indoor domestication survival rate of strawberry virus-free seedlings |
Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101946702A (en) * | 2010-08-13 | 2011-01-19 | 北京金六环农业园 | Special medium for tissue culture of strawberry stem tip and method thereof for producing detoxification seedlings |
| CN102523902A (en) * | 2012-03-17 | 2012-07-04 | 常熟市尚湖农业生态园有限公司 | High yield cultivation method for interplanting strawberries and melons |
| CN104396503A (en) * | 2014-11-10 | 2015-03-11 | 福建省农业科学院生物技术研究所 | Summer acclimation transition method for strawberry tissue culture seedlings |
| CN106106068A (en) * | 2016-06-01 | 2016-11-16 | 中国农业科学院东海农业综合试验站 | A kind of acclimation method of Fructus Fragariae Ananssae detoxic seedling |
| CN107079688A (en) * | 2017-04-22 | 2017-08-22 | 蚌埠市乔峰农业蔬菜专业合作社 | A kind of implantation methods of nuisanceless strawberry |
-
2018
- 2018-09-28 CN CN201811135977.9A patent/CN109197543A/en active Pending
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101946702A (en) * | 2010-08-13 | 2011-01-19 | 北京金六环农业园 | Special medium for tissue culture of strawberry stem tip and method thereof for producing detoxification seedlings |
| CN102523902A (en) * | 2012-03-17 | 2012-07-04 | 常熟市尚湖农业生态园有限公司 | High yield cultivation method for interplanting strawberries and melons |
| CN104396503A (en) * | 2014-11-10 | 2015-03-11 | 福建省农业科学院生物技术研究所 | Summer acclimation transition method for strawberry tissue culture seedlings |
| CN106106068A (en) * | 2016-06-01 | 2016-11-16 | 中国农业科学院东海农业综合试验站 | A kind of acclimation method of Fructus Fragariae Ananssae detoxic seedling |
| CN107079688A (en) * | 2017-04-22 | 2017-08-22 | 蚌埠市乔峰农业蔬菜专业合作社 | A kind of implantation methods of nuisanceless strawberry |
Non-Patent Citations (1)
| Title |
|---|
| 陈英等: "草莓组培苗高效移栽驯化技术的研究", 《新疆农垦科技》 * |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111109060A (en) * | 2019-11-29 | 2020-05-08 | 南京农业大学 | Method for increasing indoor domestication survival rate of strawberry virus-free seedlings |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN102119660B (en) | Method for rooting culture,seedling adaptation and transplantation of alpine rose tissue-cultured seedling in greenhouse | |
| CN102301952B (en) | Method for breeding chamomile | |
| CN107980635A (en) | A kind of Tissue-cultured apple seedling two step method for transplanting of high-survival rate | |
| CN102870680A (en) | Efficient rapid propagation technique appropriate for detoxified rabbiteye blueberries | |
| CN104885773A (en) | Method for rapidly cultivating early shaping tissue culture commodity seedlings of blueberries | |
| CN110192524A (en) | Method for in vitro rapid breeding of Zingiberaceae plant by using leaf stem and inflorescence stem hidden bud as explant | |
| CN106034722A (en) | Cutting propagation method of golden leaf plant namely weigela florida variegata | |
| CN103460971B (en) | Method for improving transplanting survival rate of trichosanthes kirilowii tissue culture seedlings | |
| CN101897297B (en) | Two-step tissue culture quick propagation method for hemerocallis | |
| CN102907326B (en) | Tissue culture propagation method for Medicagao Sativa L. | |
| CN107197746B (en) | Breeding method of cunninghamia lanceolata field excellent resources | |
| CN112586346A (en) | Tree eggplant, and cultivation method, rapid propagation method and application thereof | |
| JPS5914725A (en) | Production of plant propagating material | |
| Mirzaeva et al. | Efficiency and quality indicators of in vitro propagation of high-yielding mulberry varieties and hybrids | |
| CN103430843B (en) | A kind of pithecellobium clypearia seed seedling stem section quick breeding method for tissue culture | |
| CN108967192A (en) | A kind of Sweetpotato Viruses Elimination bottle seedling acclimatization and transplants method | |
| CN111034617B (en) | Method for breeding tea seedlings by culturing young embryo tissues of Yunnan large-leaf tea trees | |
| CN105010142A (en) | Vietnamese Aquilaria agallocha Roxb tissue culture method | |
| CN105145042B (en) | Virus-free basic potato seed soil incubation method | |
| CN103843664A (en) | Lycium exsertum tissue culture and rapid propagation method | |
| CN104303765B (en) | The high-yield planting method of the stem of noble dendrobium | |
| CN109197543A (en) | A kind of acclimation method of Plantlets of Strawberry | |
| CN103039363A (en) | Rooting medium for tissue culture seedling propagation of camellia oleifera abel and propagation method thereof | |
| CN105519440A (en) | Paraserianthes falcataria clone tissue culture method | |
| CN112616671B (en) | Method for obtaining industrial hemp single-plant tissue culture seedlings |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| RJ01 | Rejection of invention patent application after publication | ||
| RJ01 | Rejection of invention patent application after publication |
Application publication date: 20190115 |