CN109184255A - A method of utilizing microorganism grout sealing outer wall of basement crack - Google Patents
A method of utilizing microorganism grout sealing outer wall of basement crack Download PDFInfo
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- CN109184255A CN109184255A CN201811288820.XA CN201811288820A CN109184255A CN 109184255 A CN109184255 A CN 109184255A CN 201811288820 A CN201811288820 A CN 201811288820A CN 109184255 A CN109184255 A CN 109184255A
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- E—FIXED CONSTRUCTIONS
- E04—BUILDING
- E04G—SCAFFOLDING; FORMS; SHUTTERING; BUILDING IMPLEMENTS OR AIDS, OR THEIR USE; HANDLING BUILDING MATERIALS ON THE SITE; REPAIRING, BREAKING-UP OR OTHER WORK ON EXISTING BUILDINGS
- E04G23/00—Working measures on existing buildings
- E04G23/02—Repairing, e.g. filling cracks; Restoring; Altering; Enlarging
- E04G23/0203—Arrangements for filling cracks or cavities in building constructions
- E04G23/0211—Arrangements for filling cracks or cavities in building constructions using injection
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- Consolidation Of Soil By Introduction Of Solidifying Substances Into Soil (AREA)
Abstract
The present invention relates to a kind of methods using microorganism grout sealing outer wall of basement crack, belong to technical field of civil engineering.Described method includes following steps: 1) preparation of plugging material；2) grout hole is drilled through above concrete of basement exterior wall Vertical Cracks, concrete wall is run through in the grout hole hole, and the direction of grout hole is to tilt upward, and the quantity of the grout hole is even number, the grout hole vertical array；3) grout pipe is inserted into grout hole, and one end of grout pipe is inserted into the wall outer side soil body after concrete wall；Then after calcium source solution, bacterium solution being connected to two adjacent grout pipes respectively with the mixed solution of nutritive salt after injection wall in the soil body；4) it after slip casting, extracts grout pipe out, grout hole is tamped.The present invention overcomes big using viscosity when organic plugging material in the prior art using bacterium solution, nutrient salt solution and the good feature of calcium source solution permeability, is not easy the shortcomings that penetrating into crack depths.
Description
Technical field
The invention belongs to technical field of civil engineering more particularly to a kind of mechanism closure using microbiological precipitation of CaCO 3
The method of outer wall of basement leakage.
Background technique
In recent years, the underground structrue engineerings such as underground garage, underground commerce area, Underground Rail Transit are more and more, scale
Also increasing.With building waterproofing technique continuous development and China's buildings waterproof standard, specification, regulation etc. it is continuous complete
Kind, the leakage ratio of China's building construction constantly declines, and national, large area leakage problems are tentatively contained, but
The leakage problems of hypogee still allow of no optimist.It is shown according to the report that in July, 2014 China's buildings waterproof association issues, 28
A urban architecture underground leakage rate is up to 57.51%.Basement leakage processing be always puzzlement engineers and technicians it is old, big,
Difficult problem.
For the leakage problems of basement, it is organic that existing plugging material generallys use epoxy resin, polyurethanes etc.
Material.These materials are mostly toxic, easy to aging over time, and tack of materials is big, are not easy to pour into tiny split
In seam.
It is exactly to come into being in this case that microorganism induction, which generates tosca grout sealing crack technology, it is
Be made of the biochemical reaction of a series of complex: the urease that the microorganism of some basophillas can utilize itself to generate will
Urea is decomposed into NH3And CO2, as the increase for decomposing the ammonia quantity generated can cause the raising of pH in ambient enviroment, make CO2?
With CO in solution3 2-Form exist.At this moment if there is Ca around bacterium2+, negatively charged organic monolayer film in bacterial cell
Ca will constantly be chelated2+, calcium carbonate crystal will be caused to deposit.Viscosity is very low before this new material solidification, by negative pressure
It may penetrate into crack depths, do not achieve the effect that closure merely with microbe deposition minerals, and leakage can be automatically found
The position at place carries out effective antiseepage, achievees the effect that detect and block two-in-one, this seems outstanding in some complex environments
It is important.The properties such as the calcite generated using microorganism induction deposition are stablized, good with the associativity of concrete material.After reparation
The antiacid of concrete material, alkali resistant, freeze thawing resistance circulation, the abilities such as anti-carbonation and impermeability be all improved, will not be because of
The aging of material and fail.
Outer wall of basement is mostly reinforced concrete wall, and appearing in the concrete cracking on concrete wall mostly is that material is received
Vertical Cracks caused by contracting.There is expert to propose to bury grout pipe to grouting at crack in outdoor drilling, but this method can be because of ground
Lower room buried depth is deeper, and grout pipe should not accurately find crack location and failure.Patent 201110413188.9 discloses a kind of micro-
The method of biological grout sealing Basement Concrete Structures Vertical Cracks is split using the method closing being in the milk in latasuture in wall
Seam, specifically include punching, borehole cleaning, fracture faces closing, bacterium solution and nutrient salt solution preparation, perfusion bacterium solution, perfusion nutritive salt it is molten
The closure of liquid, fill orifice, bacterium solution induction calcium chloride generate tosca object crack and fill orifice can effectively fills with
It blocks.The water percolating capacity of observation after through water injection test and raining, primary fissure seam significantly reduces.But this method is in dry season without ground
The method taken when being lauched outside seepage flow can will pour into the filling in crack when the outside seepage flow of the underground water for having certain pressure in crack
Slurries wash away, and cause to block failure.Therefore a kind of grout sealing method still to play a role under seepage state is researched and developed very
It is necessary.
Summary of the invention
For above-mentioned problems of the prior art, the present invention is intended to provide a kind of utilize microorganism grout sealing underground
The method of outdoor wall crack.Present invention utilizes bacterium solution, nutrient salt solution and the good features of calcium source solution permeability, are perfused
It is real in water flow upstream side, the soil aperture that grouting liquid is filled in distress in concrete and close crack with the flowing of water
Existing effect blocks.It is big to overcome organic plugging material viscosity, is not easy the shortcomings that penetrating into crack depths.
An object of the present invention is to provide a kind of method using microorganism grout sealing outer wall of basement crack.
The second object of the present invention is to provide answering for the above-mentioned method using microorganism grout sealing outer wall of basement crack
With.
For achieving the above object, specifically, the invention discloses following technical proposals:
Firstly, the present invention discloses a kind of method using microorganism grout sealing outer wall of basement crack, including walk as follows
It is rapid:
(1) preparation of plugging material: described including calcium source solution, the preparation of nutrient salt solution and the preparation of microorganism
Microorganism is can be by the mushroom of calcium source inductive formation calcium carbonate in ambient enviroment；
(2) grout hole is drilled through above concrete of basement exterior wall Vertical Cracks, concrete is run through in the grout hole hole
Wall, and the direction of grout hole is to tilt upward, the quantity of the grout hole is even number, the grout hole vertical array；
(3) grout pipe is inserted into grout hole, and one end of grout pipe passes through insertion wall outer side soil after concrete wall
In body, the other end is located at outside concrete wall；Then by the mixed solution of calcium source solution, bacterium solution and nutritive salt respectively with it is adjacent
The connection of two grout pipes after inject after wall in the soil body；
(4) it after slip casting, extracts grout pipe out, grout hole is tamped.
Preferably, calcium source solution, nutrient salt solution and bacterium are determined using the test result in patent 201510113200.2
The type and concentration of liquid, because the source solution of patent offer, the type of nutrient salt solution and bacterium solution and concentration not only effectively mention
High carbon acid doped calcium object total output, and the early yield of deposit can be effectively improved, this is mixed for being blocked using grouting method
Solidifying soil cracking sewer is significant, and coagulation could be blocked within a short period of time by only generating a large amount of precipitations of calcium carbonate in the short period
Soil cracking seam, therefore grouting quantity and number can be reduced.
Further, in step (1), the calcium source solution selects calcium nitrate (Ca (NO3)2) solution or calcium acetate (Ca
(CH3COO)2) solution, main function is to provide calcium ion for tosca.
Further, in step (1), the concentration of the calcium source solution is 1-4mol/L, preferably 2mol/L.
Further, in step (1), the nutrient salt solution includes urea (CO (NH2)2) solution, main function be for
The growth and breeding of microorganism provide nutriment.
Further, in step (1), the concentration of the nutrient salt solution is 2-4mol/L, preferably 3mol/L.
Further, in step (1), the microorganism includes Bacillus pasteurii.The microorganism is trained using fermentor
It supports.Medium component and content are as follows: yeast extract 20g/L, ammonium sulfate 10g/L, sodium hydroxide 2g/L (adjusting pH=9) and chlorine
Change nickel 10umol/L.Incubation time is set as 20h, and temperature is set as 30 DEG C, for the oxygen for guaranteeing supply bacterium abundance, vibrates bed
Revolving speed is 210r/min.It is greater than 1 with the enzymatic activity OD600 of conductivity method detection bacterium solution after culture.
Further, in step (2), the grout hole is located at the top 1-3cm in crack.
Further, in step (2), the quantity of the grout hole is 2, diameter 0.8-1.5cm, preferably 1cm.
Further, in step (2), the grout hole and horizontal plane inclination angle are between 30~45 °.The hole of grout hole is upward
Inclination is in order to which the calcium carbonate for generation of being in the milk can also flow into grout hole and distress in concrete under flow action.
Further, in step (3), the volume ratio of the mixed solution of the calcium source solution of the injection, bacterium solution and nutritive salt
For 1-3:1-4, preferably 1:1.
Further, in step (3), the grouting pressure is greater than seepage pressure, in order to which slurries are successfully injected wall
In the soil body of external side.
Further, in step (3), the material of the grout pipe includes PVC or metal material.
Further, in step (3), the depth in the grout pipe insertion wall outer side soil body is 20~30cm.
Further, in step (3), it is 20~30cm that the grout pipe, which is located at the length outside concrete wall, and with
Hose connection, in order to which grout pipe and loading calcium source solution, bacterium solution to be connected to the device of the mixed solution of nutritive salt.
Preferably, in the device of grout pipe and the mixed solution for loading calcium source solution and grout pipe and bacterium solution and nutritive salt
Between be provided with the pump housing, in order to which solution is pumped into the wall outer side soil body.
Further, in step (3), the gap between grout pipe and grout hole is filled out with sealing materials such as rubber
Plug, prevents spillage during slip casting.
Due to the calcium carbonate of generation itself be it is white, when grouting to there is milky tosca along crack when, stop
Only it is in the milk.Further, in step (3), band color can also be added when configuring solution and chemistry will not occur with solution instead
The liquid answered.It can be easier to differentiate whether grouting liquid flows out in crack in this way, when grouting is colored to occurring along crack
When grouting liquid, stop grouting.
Further, in step (4), grout hole is tamped with fine sand.
It should be noted that finishing in slip casting, and water flow is still had in crack and grout hole after grout hole is tamped
Exudation, but as calcium carbonate constantly deposits, water flow is with grouting liquid and the calcium carbonate granule of deposition by crack, seepage through soil mass channel
And the gap in grout hole between fine sand gradually blocks.
Secondly, the present invention discloses the method using microorganism grout sealing outer wall of basement crack in underground garage, underground
Application in the fields such as shopping centre, Underground Rail Transit
Compared with prior art, the beneficial effect that the present invention obtains is:
(1) present invention utilizes bacterium solution, nutrient salt solution and the good feature of calcium source solution permeability, it is poured into water flow
Grouting liquid is filled in distress in concrete with the flowing of water and in the soil aperture in crack, realized effective by upstream side
It blocks, overcomes the shortcomings that penetrating into crack depths greatly, is not easy using viscosity when organic plugging material in the prior art well.
(2) it in method of the invention, is constructed using punching indoors, this method is not only easily found breakthrough, phase
Than the way more convenient and quicker for beating grout hole downwards on ground；Importantly, the present invention is set by tilting grout hole upwards
It sets in the top in crack, the calcium carbonate that grouting can be made to generate well flows into grout hole and coagulation soil cracking by the effect of water flow
In seam, fracture is blocked, and the design method of the position of this drilling of the invention solves searching breakthrough, then big face
Product slip casting blocks bring blindness, and the position of slip casting is made to become accurate, time saving and energy saving, have mark that can follow.
(3) whether method of the invention is by not having the condition of leakage to be limited, because can have artesian water infiltration when having seepage flow
Can find leak source in the case where stream by water flow, when no seepage flow can also flowing by from the liquid of the entrance of slip casting to gap,
The calcium carbonate for generating grouting enters gap and realizes closure, becomes apparent from than other microorganism grouting method advantages, through overtesting, originally
Seepage flow can disappear the method for invention in 3~5 days after the completion of slip casting, efficiently easy.
Detailed description of the invention
The accompanying drawings constituting a part of this application is used to provide further understanding of the present application, and the application's shows
Meaning property embodiment and its explanation are not constituted an undue limitation on the present application for explaining the application.
Fig. 1 is that outer wall of basement has seepage flow diagrammatic cross-section at crack.
Fig. 2 is the schematic diagram for opening up grout hole and installing grout pipe.
Fig. 3 is the schematic diagram for connecting grouting pump and pressure grouting.
Fig. 4 is the schematic diagram extracting grout pipe and being blocked with fine sand.
Fig. 5 is the structural schematic diagram that position is arranged in comparative example grout hole.
Fig. 6 is the practice of construction figure that position is arranged in comparative example grout hole.
Fig. 7 is the test result figure of comparative example grouting result.
Appended drawing reference respectively represents in figure: the seepage flow road of the outer soil body of 1- Vertical Cracks, 2- concrete exterior wall, 3- wall, 4- water
Diameter, 5- calcium source grout pipe, 6- bacterium solution and nutritive salt mixed liquor grout pipe, 7- hose, 8- calcium source pressure grouting pump, 9- bacterium solution and battalion
Support salt mixed liquor pressure grouting pump, 10- grouting liquid and the tosca of generation, 11- fine sand.
Specific embodiment
It is noted that following detailed description is all illustrative, it is intended to provide further instruction to the application.Unless another
It indicates, all technical and scientific terms used herein has usual with the application person of an ordinary skill in the technical field
The identical meanings of understanding.
It should be noted that term used herein above is merely to describe specific embodiment, and be not intended to restricted root
According to the illustrative embodiments of the application.As used herein, unless the context clearly indicates otherwise, otherwise singular
Also it is intended to include plural form, additionally, it should be understood that, when in the present specification using term "comprising" and/or " packet
Include " when, indicate existing characteristics, step, operation, device, component and/or their combination.
As background technique is introduced, existing method is the side taken when not having the outside seepage flow of underground water in dry season
Method can wash away the grouting liquid for pouring into crack when the outside seepage flow of the underground water for having certain pressure in crack, cause to block failure.
Therefore it is very necessary to research and develop a kind of grout sealing method still to play a role under seepage state.For this purpose, the present invention proposes one
Kind utilize microorganism grout sealing outer wall of basement crack method, with reference to the accompanying drawing and specific embodiment to the present invention into
One step explanation.
In the following example, the microorganism is Bacillus pasteurii, using fermentation tank culture, medium component and content
Are as follows: yeast extract 20g/L, ammonium sulfate 10g/L, sodium hydroxide 2g/L (adjusting PH=9) and nickel chloride 10umol/L.When culture
Between be set as 20h, temperature is set as 30 DEG C, for guarantee supply bacterium abundance oxygen, oscillation bed revolving speed be 210r/min.Culture
After with conductivity method detection bacterium solution enzymatic activity OD600 be greater than 1.
Embodiment 1
As shown in Figs 1-4, a method of using microorganism grout sealing outer wall of basement crack, include the following steps:
(1) preparation of plugging material: (nutritive salt is molten for the urea of calcium nitrate solution (calcium source), 3mol/L including 2mol/L
Liquid) and microorganism, the microorganism is can be by the mushroom of calcium source inductive formation calcium carbonate in ambient enviroment；
(2) grout hole is drilled through at the top 2cm of concrete of basement exterior wall Vertical Cracks 1, the grout hole hole is run through
Concrete wall 2, grout hole and horizontal plane inclination angle are 45 °, and quantity is 2, respectively calcium source grout pipe 5 and bacterium solution and nutrition
Salt mixed liquor grout pipe 6, the diameter of two grout holes is 1.0cm, and two grout hole vertical non-alignments arrange；
(3) grout pipe is inserted into grout hole, and one end of grout pipe passes through insertion wall outer side soil after concrete wall
In body 3, and the depth in the grout pipe insertion wall outer side soil body is 25cm, and the other end is located at outside concrete wall；The filling
The part that slurry pipe is located at outside concrete wall is connect with hose 7, the hose and loading calcium source solution and grout pipe and bacterium
Calcium source pressure grouting pump 8, bacterium solution and nutritive salt mixed liquor pressure is provided between the device of the mixed solution of liquid and nutritive salt to fill
Stock pump 9 injects wall after being then connected to calcium source solution, bacterium solution with two adjacent grout pipes respectively with the mixed solution of nutritive salt
Afterwards in the soil body, and it is 25cm that grout pipe, which is located at the length outside concrete wall, the calcium source solution of injection, bacterium solution and nutritive salt
The volume ratio of mixed solution is 1:1；The grouting pressure is greater than seepage pressure, in order to successfully will be on the outside of slurries injection wall bodies
In the soil body；The material of the grout pipe is PVC；Due to the calcium carbonate of generation itself be it is white, when grouting to along crack occur
When milky tosca, stop grouting；
(4) it after slip casting, extracts grout pipe out, is tamped grout hole with fine sand 11, still had after grout hole is tamped
Water flow is oozed out in crack and grout hole, but as calcium carbonate constantly deposits, water flow with grouting liquid and deposition calcium carbonate
Grain 10 gradually blocks in the gap between fine sand in crack, seepage through soil mass channel and grout hole in (direction of percolation path 4 in figure), passes through
It crosses test to obtain, seepage flow stops at about 3 days, and gap is blocked completely, and repetition measurement at one month, gap is without seepage flow, plugging effect
Well.
Embodiment 2
A method of using microorganism grout sealing outer wall of basement crack, include the following steps:
(1) preparation of plugging material: (nutritive salt is molten for the urea of calcium acetate solution (calcium source), 2mol/L including 1mol/L
Liquid) and microorganism, the microorganism is can be by the mushroom of calcium source inductive formation calcium carbonate in ambient enviroment；
(2) grout hole is drilled through at the top 1.0cm of concrete of basement exterior wall Vertical Cracks, the grout hole hole is run through
Concrete wall, grout hole and horizontal plane inclination angle are 30 °, and quantity is two groups, every group 2, respectively calcium source grout pipe and bacterium
Liquid and nutritive salt mixed liquor grout pipe, the diameter of two grout holes is 0.8cm, and two grout hole vertical non-alignments arrange；
(3) grout pipe is inserted into grout hole, and one end of grout pipe passes through insertion wall outer side soil after concrete wall
In body, and the depth in the grout pipe insertion wall outer side soil body is 30cm, and the other end is located at outside concrete wall；The grouting
The part that pipe is located at outside concrete wall connect with hose, the hose and loading calcium source solution and grout pipe and bacterium solution and
Calcium source pressure grouting pump, bacterium solution and nutritive salt mixed liquor pressure grouting pump are provided between the device of the mixed solution of nutritive salt,
Then the soil body after injection wall after being connected to calcium source solution, bacterium solution with two adjacent grout pipes respectively with the mixed solution of nutritive salt
In, and it is 20cm that grout pipe, which is located at the length outside concrete wall, the mixing of the calcium source solution of injection, bacterium solution and nutritive salt is molten
The volume ratio of liquid is 3:4；The grouting pressure is greater than seepage pressure, in order to successfully by the soil body on the outside of slurries injection wall body
In；The material of the grout pipe is PVC；Due to the calcium carbonate of generation itself be it is white, when grouting to along crack occur it is milky white
When color tosca, stop grouting；
(4) it after slip casting, extracts grout pipe out, is tamped grout hole with fine sand, water is still had after grout hole is tamped
Stream oozes out in crack and grout hole, but as calcium carbonate constantly deposits, water flow with grouting liquid and deposition calcium carbonate granule
Gap between fine sand in crack, seepage through soil mass channel and grout hole is gradually blocked, is obtained by test, seepage flow at about 4 days
Stop, gap blocked completely, and repetition measurement at one month, without seepage flow, plugging effect is good in gap.
Embodiment 3
A method of using microorganism grout sealing outer wall of basement crack, include the following steps:
(1) preparation of plugging material: (nutritive salt is molten for the urea of calcium acetate solution (calcium source), 4mol/L including 4mol/L
Liquid) and microorganism, the microorganism is can be by the mushroom of calcium source inductive formation calcium carbonate in ambient enviroment；
(2) grout hole is drilled through at the top 3.0cm of concrete of basement exterior wall Vertical Cracks, the grout hole hole is run through
Concrete wall, grout hole and horizontal plane inclination angle are 40 °, and quantity is two groups, every group 2, respectively calcium source grout pipe and bacterium
Liquid and nutritive salt mixed liquor grout pipe, the diameter of two grout holes is 1.5cm, and two grout hole vertical non-alignments arrange；
(3) grout pipe is inserted into grout hole, and one end of grout pipe passes through insertion wall outer side soil after concrete wall
In body, and the depth in the grout pipe insertion wall outer side soil body is 20cm, and the other end is located at outside concrete wall；The grouting
The part that pipe is located at outside concrete wall connect with hose, the hose and loading calcium source solution and grout pipe and bacterium solution and
Calcium source pressure grouting pump, bacterium solution and nutritive salt mixed liquor pressure grouting pump are provided between the device of the mixed solution of nutritive salt,
Then the soil body after injection wall after being connected to calcium source solution, bacterium solution with two adjacent grout pipes respectively with the mixed solution of nutritive salt
In, and it is 30cm that grout pipe, which is located at the length outside concrete wall, the mixing of the calcium source solution of injection, bacterium solution and nutritive salt is molten
The volume ratio of liquid is 2:3；The grouting pressure is greater than seepage pressure, in order to successfully by the soil body on the outside of slurries injection wall body
In；The material of the grout pipe is stainless steel；Due to the calcium carbonate of generation itself be it is white, when grouting to along crack occur cream
When white calcium carbonate deposits, stop grouting；
(4) it after slip casting, extracts grout pipe out, is tamped grout hole with fine sand, water is still had after grout hole is tamped
Stream oozes out in crack and grout hole, but as calcium carbonate constantly deposits, water flow with grouting liquid and deposition calcium carbonate granule
Gap between fine sand in crack, seepage through soil mass channel and grout hole is gradually blocked, is obtained by test, seepage flow at about 5 days
Stop, gap blocked completely, and repetition measurement at one month, without seepage flow, plugging effect is good in gap.
Comparative example
Before improvement, the method for arranging of grout pipe when the present invention utilizes microorganism grout sealing outer wall of basement crack are as follows:
There is crack location to punch the grouting (as shown in Figure 5, Figure 6) into crack on the outside of crack, although being formed at crack after grouting
Waterstop, but since grout pipe is not above crack, it causes to fail effectively to block at the top of crack, after grouting 4 months still
There is water flow exudation at the top of crack, as shown in Figure 6, wherein Fig. 7 (a) is before being in the milk, and Fig. 7 (b) is that grouting just terminates, Fig. 7
(c) after for grouting 4 months.
The foregoing is merely preferred embodiment of the present application, are not intended to limit this application, for those skilled in the art
For member, various changes and changes are possible in this application.Within the spirit and principles of this application, it is made it is any modification,
Equivalent replacement, improvement etc., should be included within the scope of protection of this application.
Claims (10)
1. a kind of method using microorganism grout sealing outer wall of basement crack, it is characterised in that: the method includes as follows
Step:
(1) preparation of plugging material: including calcium source solution, the preparation of nutrient salt solution and the preparation of microorganism, micro- life
Object is can be by the mushroom of calcium source inductive formation calcium carbonate in ambient enviroment；
(2) grout hole is drilled through above concrete of basement exterior wall Vertical Cracks, concrete wall is run through in the grout hole hole,
And the direction of grout hole is to tilt upward, the quantity of the grout hole is even number, the grout hole vertical array；
(3) grout pipe is inserted into grout hole, and one end of grout pipe is inserted into the wall outer side soil body after concrete wall,
The other end is located at outside concrete wall；Then by the mixed solution of calcium source solution, bacterium solution and nutritive salt respectively with adjacent two
After a grout pipe connection after injection wall in the soil body；
(4) it after slip casting, extracts grout pipe out, grout hole is tamped.
2. utilizing the method in microorganism grout sealing outer wall of basement crack as described in claim 1, it is characterised in that: step
(1) in, the calcium source solution selects calcium nitrate or calcium acetate solution；
Alternatively, the concentration of the calcium source solution is 1-4mol/L, preferably 2mol/L in step (1)；
Alternatively, in step (1), the nutrient salt solution includes urea liquid, and main function is for the growth of microorganism and numerous
Offer nutriment is provided；
Alternatively, the concentration of the nutrient salt solution is 2-4mol/L, preferably 3mol/L in step (1).
3. utilizing the method in microorganism grout sealing outer wall of basement crack as described in claim 1, it is characterised in that: step
(1) in, the microorganism includes Bacillus pasteurii；The microorganism uses fermentation tank culture；Preferably, the culture Pasteur
The medium component and content of bacillus are as follows: yeast extract 20g/L, ammonium sulfate 10g/L, sodium hydroxide 2g/L and nickel chloride
10umol/L；Incubation time is set as 20h, and temperature is set as 30 DEG C, for the oxygen for guaranteeing supply bacterium abundance, vibrates bed revolving speed
For 210r/min, it is greater than 1 with the enzymatic activity OD600 of conductivity method detection bacterium solution after culture.
4. utilizing the method in microorganism grout sealing outer wall of basement crack as described in claim 1, it is characterised in that: step
(2) in, the grout hole is located at the top 1-3cm in crack.
5. the method according to any one of claims 1-4 using microorganism grout sealing outer wall of basement crack, feature
Be: in step (2), the grout hole and horizontal plane inclination angle are between 30~45 °.
6. utilizing the method in microorganism grout sealing outer wall of basement crack as claimed in claim 4, it is characterised in that: step
(2) in, the quantity of the grout hole is 2, diameter 0.8-1.5cm, preferably 1cm.
7. utilizing the method in microorganism grout sealing outer wall of basement crack as described in claim 1, it is characterised in that: step
(3) in, the volume ratio of the mixed solution of the calcium source solution of the injection, bacterium solution and nutritive salt is 1-3:1-4, preferably 1:1；
Alternatively, the grouting pressure is greater than seepage pressure in step (3), in order to successfully by soil on the outside of slurries injection wall body
In body；
Alternatively, the material of the grout pipe includes PVC or metal material in step (3).
8. utilizing the method in microorganism grout sealing outer wall of basement crack as described in claim 1, it is characterised in that: step
(3) in, the depth in the grout pipe insertion wall outer side soil body is 20~30cm；
Alternatively, it is 20~30cm that the grout pipe, which is located at the length outside concrete wall, and is connect with hose in step (3)；
Alternatively, being set between grout pipe and the device for the mixed solution for loading calcium source solution and grout pipe and bacterium solution and nutritive salt
It is equipped with the pump housing；
Alternatively, being clogged with sealing materials such as rubber to the gap between grout pipe and grout hole in step (3).
9. utilizing the method in microorganism grout sealing outer wall of basement crack as described in claim 1, it is characterised in that: step
(3) it in, is added when configuring solution with color and the liquid that will not be chemically reacted with solution；Alternatively, being used in step (4)
Fine sand tamps grout hole.
10. if the described in any item methods using microorganism grout sealing outer wall of basement crack of claim 1-9 are in underground
Application in the fields such as garage, underground commerce area, Underground Rail Transit.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201811288820.XA CN109184255A (en) | 2018-10-31 | 2018-10-31 | A method of utilizing microorganism grout sealing outer wall of basement crack |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201811288820.XA CN109184255A (en) | 2018-10-31 | 2018-10-31 | A method of utilizing microorganism grout sealing outer wall of basement crack |
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| Publication Number | Publication Date |
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| CN109184255A true CN109184255A (en) | 2019-01-11 |
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Cited By (7)
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| CN109779011A (en) * | 2019-01-23 | 2019-05-21 | 山东建筑大学 | The method and structure of microorganism grouting anchor connection concrete component longitudinal reinforcement |
| CN109838113A (en) * | 2019-03-04 | 2019-06-04 | 山东建筑大学 | Microorganism induction generates the method that calcium carbonate blocks Basement Crack |
| CN110513111A (en) * | 2019-08-14 | 2019-11-29 | 中国矿业大学(北京) | A kind of cementing lane formula filling coal mining method of separation of solid and liquid filling type microorganism |
| CN112198025A (en) * | 2020-09-29 | 2021-01-08 | 中国海洋大学 | Test method for plugging rock cracks based on microbial grouting technology |
| CN113685058A (en) * | 2021-09-14 | 2021-11-23 | 山东建筑大学 | Method for replacing low-strength mortar of wall by utilizing microbial grouting |
| WO2022027958A1 (en) * | 2020-08-07 | 2022-02-10 | 山东建筑大学 | Microbial grouting anchoring method for intracavity grouting and anchoring structure |
| CN114271057A (en) * | 2021-12-29 | 2022-04-05 | 北京建工环境修复股份有限公司 | Soil deep layer separation and restoration technology and application thereof |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN112198025A (en) * | 2020-09-29 | 2021-01-08 | 中国海洋大学 | Test method for plugging rock cracks based on microbial grouting technology |
| CN113685058A (en) * | 2021-09-14 | 2021-11-23 | 山东建筑大学 | Method for replacing low-strength mortar of wall by utilizing microbial grouting |
| CN114271057A (en) * | 2021-12-29 | 2022-04-05 | 北京建工环境修复股份有限公司 | Soil deep layer separation and restoration technology and application thereof |
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