CN106884424A - A kind of device and construction method that microorganism solidification is carried out in thin silt - Google Patents
A kind of device and construction method that microorganism solidification is carried out in thin silt Download PDFInfo
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- CN106884424A CN106884424A CN201710227568.0A CN201710227568A CN106884424A CN 106884424 A CN106884424 A CN 106884424A CN 201710227568 A CN201710227568 A CN 201710227568A CN 106884424 A CN106884424 A CN 106884424A
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- E—FIXED CONSTRUCTIONS
- E02—HYDRAULIC ENGINEERING; FOUNDATIONS; SOIL SHIFTING
- E02D—FOUNDATIONS; EXCAVATIONS; EMBANKMENTS; UNDERGROUND OR UNDERWATER STRUCTURES
- E02D3/00—Improving or preserving soil or rock, e.g. preserving permafrost soil
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Abstract
The present invention relates to a kind of device and construction method that microorganism grouting is carried out in thin silt, including bacterium solution culture tank, microorganism slurries mixer, calcium mixture source slot, microorganism slurries mixer is connected with bacterium solution culture tank, calcium mixture source slot respectively by transfer pipeline, and rig is utilized and crept into pending stratum bottom with managing and protecting wall pore-forming mode；The high pressure Grout-jetting pipe under the bottom of protection casing；The space between protection casing and high pressure Grout-jetting pipe is closed by way of high pressure Grout-jetting pipe pulp section mould bag obstruction；Microorganism slurries are carried out pure pressure type pouring construction by high pressure chemistry grouting pump by high-pressure grouting pipe and high pressure Grout-jetting pipe.Present slurry reasonable mixture ratio, the good penetrability in thin silt, diffusion is uniform, it is workable, effect reliability, cost savings can be widely applied to the based process field of Geotechnical Engineering, soil body intensity under microorganism solidification is improved, permeability declines, for the grouting technique Ground Treatment based on microbial biochemical mechanism provides new direction.
Description
Technical field
The present invention relates to the technical field of foundation treatment of Geotechnical Engineering, and in particular to one kind carries out micro- life in thin silt
The device and construction method of thing solidification.
Background technology
In great demand to all kinds of infrastructure constructions because Urbanization in China is constantly accelerated, building engineering field is whole
Body is enlivened, and can all be run into all kinds of engineering constructions can not directly meet the adverse soil mass of engine request, wherein fine sand layer most
For common.
So-called Extra-fine sand refers to that particle of the particle diameter more than 0.075mm exceedes the 50% of all-mass, and particle diameter is less than 0.25mm
Particle, its content exceed all-mass 50% sand.Its engineering properties between sandy soil and cohesive soil, natural
Loosely organized under state, density is relatively low, in the presence of deadweight can densification, the engineering properties of Extra-fine sand includes：(1) powder
The particle diameter distribution of fine sand is uniform, and particle size range very little, loosely organized, under extraneous load action, is easily deformed；(2) powder is thin
Sand body is stablized and bearing capacity in itself mainly by the intergranular friction power maintenance depending on intergranular normal pressure, in the presence of being sheared
The easy unstability of the soil body；(3) in the case of by water saturation, rate of deformation of the Extra-fine sand under load action is increased, by Deformation control
Bearing capacity it is relatively low；(4) Extra-fine sand infiltration coefficient is generally l0-3Cm/s or so, belongs to medium water penetration, impermeabilisation ability compared with
Difference, easily produces the seepage failures such as stream soil, piping；(5) saturation Extra-fine sand produces liquefaction existing under reciprocal shear action, easily
As.Fine sand layer is also easy to produce seepage under head pressure effect, and intensity is relatively low, and the safety to all kinds of buildings constitutes potential danger
Evil, therefore it is the technical barrier that many underground engineerings have to solve that effective anti-seepage reinforcing is carried out to fine sand layer.
Grouting technique is one of important means of fine sand layer anti-seepage reinforcing.Traditional fine sand layer impervious consolidation skill
Art is mostly to inject in fine sand layer hole Binder Materials using big machinery to reach anti-seepage reinforcing effect.It is common in engineering
Injecting paste material has：Cement plasmoid humor（Cement mortar, cement-sodium silicate slurry etc.）, waterglass-calcium chloride slurries, waterglass-sodium aluminate
Slurries, resin plasmoid humor and propionic acid plasmoid humor, wooden amine slurries etc..Fine sand layer compact structure, general graininess note
Pulp material, such as cement class material are difficult to note into effect is poor, and the material such as lime, flyash needs a large amount of fossil fuels when producing
Burning, produce a large amount of greenhouse gases, high energy consumption, environmental pollution are serious；Waterglass-calcium chloride and waterglass-sodium aluminate pulp
Grouting process is complicated, poor operability, and slurries raw material sources are difficult, and engineering is low using rate；Resin plasmoid humor and propionic acid acyl
Amine slurries are relatively costly, are not suitable for engineering large-scale use；Wooden amine slurries are toxic, serious using rear environmental pollution,
Be not suitable for engineering to use.Waterglass and the slurries with waterglass as host using relatively more early, can be divided into alkaline water glass pulp and
Acidic water glass slurries, alkaline waterglass sand fixing strength is very low and is separated out with alkali, easily causes the alkaline pollution in environment；Acid
Property waterglass performance of although fixing the sand it is slightly higher, stability preferably, but condenses too fast after slip casting, diffusion extreme difference, and water glass
The grouting material of glass class its poor durability, is not suitable for being used in permanent works.
Over nearly 20 years, intersecting on subject to process and show great advantage on these underlying issues, using micro- life
Thing carries out the soil body and is modified, and has larger development from theory to technology, using microorganism to ecological environment and sustainable development
There is profound significance.Permanent biology is there is in foundation soil, 10 are at least contained in closely matrix section, per kilogram soil12's
Microorganism, in the range of the exemplary depth of basement process（Such as 2~30m）, microbial population is along depth meeting in per kilogram soil
Drop to 1011~106, soil is considered as a kind of ecosystem living, its influence to Characteristics of Geotechnical Engineering for a long time is ignored.
Biological activity present in soil layer and biochemical reaction make it possible that carrying out soil layer using nature and spontaneous method reinforces,
Mitchell ＆Santamarina clearly discussed bioprocess and are acted in Geotechnical Engineering for the first time in 2005；State of the U.S.
Research committee of family（NRC）This problem has been listed in 21 century important subject in 2006；First world in 2007
Biological Geotechnical Engineering seminar has carried out interdisciplinary exchange to this emerging field；The Second Committee biological soil engineering of in September, 2011
And effect seminar of meeting is held in Cambridge University；Other international seminars corresponding with various countries are held so that the field is ground
Study carefully and make much progress.
Soil layer is reinforced using the metabolic function of microorganism or its active procedure in soil layer, in specific ring
Under border and nutritional condition, certain micro-organisms present in ground can quickly separate out various ore deposits by metabolism or degradation
Thing is crystallized, such as carbonate, phosphate, oxide, sulfide, siliceous sinter and extracellular polymeric.Microorganism induction calcium carbonate sinks
Product technology（MICP）Using microbial biochemical processes such as hydrolysis of urea, sulfate reduction, fatty acid fermentation, denitrifications to soil
Layer carries out impervious consolidation.According to having studied, the calcium carbonate quantity that hydrolysis of urea process is induced is most, consolidation effect
Preferably, its basic process is：Bacteria metabolism produces urase, such as there is urea and calcium salt, nutrient solution in surrounding environment, then
Urase hydrolysis urea generation ammonia and carbon dioxide, the negatively charged organic matter in bacterial cell membrane interface constantly adsorb positively charged
The calcium ion of lotus, calcium carbonate, wherein microbial life periodic process are deposited with ureagenetic carbanion mineralising is hydrolyzed
There is the biomineralization of complexity between the organic product and inorganic mineral of middle metabolism, the crystallization of inorganic phase is strictly given birth to
Thing secretes the control of organic matter.However, microorganism slurries are poured into deep silty-fine sand layer plays the report of reinforcement effect and few
See.
The content of the invention
It is an object of the invention to provide a kind of cheap, environment-protecting asepsis and construction effect is reliable to deep silty-fine sand
Stratum carries out the device and construction method of microorganism solidification.
One aspect of the present invention provides a kind of device that microorganism solidification is carried out in thin silt, including bacterium solution culture tank
12nd, microorganism slurries mixer 10, calcium mixture source slot 11, microorganism slurries mixer 10 by transfer pipeline 13 respectively with bacterium solution
Culture tank 12, calcium mixture source slot 11 are connected,
, using being crept into the bottom on pending stratum with managing and protecting wall pore-forming mode, the diameter that boring aperture should meet grouting will for rig
Ask, 108mm is not preferably less than typically；
The high pressure Grout-jetting pipe 6 under the bottom of protection casing 4；
Closing protection casing 4 and high pressure Grout-jetting pipe 6 by way of the obstruction of the pulp of high pressure Grout-jetting pipe 6 section grout sealing mould bag 2
Between space；
Microorganism slurries are irrigated and applied by high pressure chemistry grouting pump 9 by high-pressure grouting pipe 7 and high pressure Grout-jetting pipe 6 using pure pressure type
Work.
Further, the volume of bacterium solution culture tank 12 is not less than 1m3。
Further, the capacity of microorganism slurries mixer 10 is not less than 500L, and mixing speed is less than 100r/min.
Further, the high pressure Grout-jetting pipe 6 and high-pressure grouting pipe 7 use high-pressure grouting pipe fitting, caliber to be not more than 50mm,
Maximum bearing capacity is 10MPa.
Further, the high pressure chemistry grouting pump 9, discharge capacity is 0~50L/min during perfusion microorganism slurries, maximum reachable
50L/min, maximum pump intake pressure is 6MPa.
Another aspect of the present invention is also provided and the construction method of microorganism solidification is carried out using said apparatus, including following step
Suddenly：
Step 1）Rig creeps into thin silt 3, using with manage and protect wall creep into the bottom on pending stratum pull out 0~
10cm, and the high pressure Grout-jetting pipe 6 under the bottom of protection casing 4；Hole inner sealing is carried out using form sack grouting, by high pressure Grout-jetting pipe 6
And the space closing between protection casing 4, solve microorganism slurries in the hole on the difficulty pulled out on high-pressure grouting pipe 7 caused by string
Inscribe and greatly reduce in hole, manage in account for slurry；
Step 2）Preparing microorganism slurries；
Step 3）With high pressure chemistry grouting pump 9 by the step 2）Middle microorganism slurries are pumped into selected by high pressure Grout-jetting pipe 6
Grout compartment, under the effect of the pressure of high pressure chemistry grouting pump 9, microorganism slurries are progressively spread, and grouting pressure is also stepped up and reached
The grouting pressure of setting, the injection rate of slurries will be gradually reduced, until reaching the injection rate of setting, this section can terminate；
Wherein grouting pressure be 3MPa, control slurries injection rate be 2~10L/min, at the end of injection rate can typically be set to
0.5L/min；
Step 4）By protection casing 4 together with high pressure Grout-jetting pipe 6 using being pulled out on drawbench 5, according to the situation of injection rate, it is slow on pull out
The supreme grout compartments of 5~50cm；
Repeat step 3）~4）, until the grout compartment of institute pending processing thin silt 3 all completes the grouting of microorganism slurries；
Step 5）After pending grout compartment all terminates, unloaded after rinsing high pressure Grout-jetting pipe 6, then mixer, pump, pipeline are entered
Row cleaning.Next hole is moved to be irrigated.This hole is that can reach age requirement after 7 ~ 14d is coagulated.
Further, the step 2）Including：
Step a, preparing microorganism bacterium solution, the microbial inoculum is made up of Bacillus pasteurii bacterium solution and nutrient solution, wherein,
Contain 15~25g of yeast extract per 1L nutrient solutions,（NH4）2SO4The Tris of 8~12g, 0.13mol, and calcium mixture liquid, adjust
Section bacterium solution pH value is 6.24, to adapt to Bacillus pasteurii growth；Wherein, calcium mixture liquid is by urea, calcium nitrate and calcium chloride group
Into mol ratio is 2:1:1；
Step b, 24h is cultivated by Bacillus pasteurii in nutrient solution；
Step c, 2~3min is mixed by the microorganism mixed liquor in step b in microorganism slurries mixer 10.
Further, calcium mixture liquid product and Bacillus pasteurii bacterium solution volume ratio are 1.5~2 in the step a:1.
Further, the inoculative proportion of the Bacillus pasteurii bacterium solution is 10%, 28 DEG C~35 DEG C of cultivation temperature.
Further, it is described to carry out the method for microorganism solidification using the filling from bottom to top, being progressively segmented in thin silt 3
Slurry construction method, infiltration can be targetedly segmented to thin silt 3 and spread；Maximum grouting segment length is no more than 0.5m, pulls out
According to slurry situation is eaten during pipe, upper pulling degree is 5cm ~ 50cm, the different grouting segment length of unrestricted choice.
Further, microorganism slurries apparent viscosity is generally less than 5mPa.s, and density is slightly larger than 1.0g/cm3；The solid sand bodies of 7d without
Confined compressive strength is more than 3MPa；The solid sand body unconfined compressive strengths of 28d are more than 5MPa, and solid sand body infiltration coefficient is less than 5.0 ×
10-5cm/s。
Further, filling process is using grouting pressure and double limit control technologies of slurries injection rate, you can ensure microorganism
The osmotic effect and range of scatter of slurries, waste prevented also from excessive slurries are produced.Specific double limit controlling values should be by ground
Matter situation, design requirement and construction requirement determine.
Further, comprehensive analysis is carried out according to factors such as engine request, strata condition and perfusion situations, between certain hole
Away from being drilled, the microorganism for completing single grout hole according to above-mentioned steps irrigates, and can form complete seepage proof curtain or reinforcing
Region, so as to realize the anti-seepage reinforcing to the thin silt that need to be processed.
Beneficial effects of the present invention are embodied in：
A kind of device and construction method for carrying out microorganism solidification in thin silt of the present invention can further promote green filling
The development of slurry technology, the microorganism slurries of use are complete solution slurry material, reasonable mixture ratio, the permeability in thin silt
Good, diffusion is uniform, and consolidation effect is environmentally friendly energy conservation type technique substantially.Construction technology is simple, and strong operability is low
Energy consumption, disturb small, effect reliability, cost savings, can be widely applied to Geotechnical Engineering field various submerged structures and basis,
In the impervious leakage-stopping engineering of the thin silts such as tunnel, bridge pier and cofferdam, soil body intensity under microorganism solidification is improved
Decline with permeability, the present invention provides new direction for the grouting technique Ground Treatment of microbial biochemical mechanism.
Other features and advantages of the present invention will illustrate in the following description, and partly become from specification
It is clear that or being understood by implementing the present invention.The main object of the present invention and further advantage can be by specification, power
Specifically noted scheme is realized and obtained in sharp claim.
Brief description of the drawings
The present invention will be further described in detail below in conjunction with the accompanying drawings.
Fig. 1 is the construction method schematic diagram that microorganism solidification is carried out in thin silt.
Reference：1- grout holes, 2- closure moulds bag, 3- thin silts, 4- protection casings, 5- drawbenches, 6- high pressures
Grout-jetting pipe, 7- high-pressure groutings pipe, 8- return pipes, 9- high pressure chemistries grouting pump, 10- microorganism slurries mixer, 11- mixing calcium sources
Groove, 12- bacterium solutions culture tank, 13- transfer pipelines, 14- bacterium solutions delivery pump, 15- calcium sources delivery pump, 16- grouting buckets.
Specific embodiment
In the present invention, it is by micro- life to the operation principle that thin silt carries out anti-seepage reinforcing using microorganism grouting
Come what is realized, microorganism decomposites a kind of urease to thing metabolic intermediate calcium carbonate, and urease can hydrolyze urea and produce carbon
Acid ion, while the negatively charged organic matter in microbial bacterial cell membrane interface constantly adsorbs positively charged calcium ion,
Carbanion goes out calcium carbonate with calcium ion deposition, and consolidation effect is reached eventually through calcium carbonate.Microorganism induction calcium carbonate, bacterium
Liquid concentration, bacteria solution active, bacterium solution monomer reactivity are the influence factors of evaluation solidification effect, active microbial inoculum higher,
Its induction calcium carbonate ability is stronger, and then it is also more preferable to reinforce sand effect.
Microorganism slurries are solution-type slurries, and its apparent viscosity is generally less than 5mPa.s, are filled according to solution-type Newtonian fluid
The infiltration of slurry and diffusion mechanism, allow microorganism slurries to be permeated in fine sand layer, spread, and reach diffusion length remote enough
Fine sand layer space is sufficiently uniformly filled, and calcium carbonate is produced under the effect of itself bacteria metabolism, filling powder is thin
Space between layer of sand, it is then cemented together, the solid sand body with some strength is formed, so as to reach the purpose of anti-seepage reinforcing,
Its solid sand body 28d compression strength can be to more than 5MPa after general reinforcing, and infiltration coefficient is less than 5.0 × 10-5Below cm/s.
Representative microbial slurries grouting construction process is as follows：
（1）Thin silt 3 is crept into using pneumatic drilling machine, is crept into the bottom on pending stratum using with managing and protecting wall 4, brill of moving away
Machine, grout hole 1 can discharge hole wait to fill;
（2）By suitable microorganism fungus kind（Such as Bacillus pasteurii, numbering ATCC11859）In condition of compatibility（Inoculative proportion, temperature
Degree and pH environment）Under be trained microbial inoculum, it is stand-by；
（3）Mix in microorganism slurries mixer 10 with the calcium mixture liquid of urea, calcium nitrate and calcium chloride, stir after form micro-
Biology grouting slurries；
（4）To wait to fill on the protection casing 4 in drilling and pull out 0~10cm, the high pressure Grout-jetting pipe 6 under the bottom of protection casing 4；Utilize
Form sack grouting carries out hole inner sealing, and the space of high pressure Grout-jetting pipe 6 and protection casing 4 is closed；
（5）Microorganism grouting slurries are pumped into selected grout compartment, micro- life by high pressure Grout-jetting pipe 6 with high pressure chemistry grouting pump 9
Thing grouting slurries are complete solution slurry materials, can be permeated in thin silt, spread.In high pressure chemistry grouting pump 9
Under pressure effect, microorganism slurries progressively spread, and grouting pressure also steps up the grouting pressure for reaching setting, the injection of slurries
Rate will be gradually reduced, until reaching the injection rate of setting, this section can terminate；
（6）By protection casing 4 together with high pressure Grout-jetting pipe 6 using being pulled out on drawbench 5, according to the situation of injection rate, it is slow on pull out 5 ~
The supreme grout compartments of 50cm, repeat the process of the above, until institute's pending processing Extra-fine sand grout compartment all completes microorganism slurries
Grouting；
（7）After pending grout compartment all terminates, to mixer, pump（High pressure chemistry grouting pump 9, bacterium solution delivery pump 14, calcium source
Delivery pump 15）, pipeline etc. cleaned.Next hole is moved to be irrigated.This hole is that can reach age requirement after 7 ~ 14d is coagulated;
（8）Comprehensive analysis is carried out according to factors such as engine request, strata condition and perfusion situations, with certain pitch of holes（Typically
It is 2 ~ 3m）Drilling construction is carried out, according to above-mentioned steps（1）~（7）The microorganism perfusion of single grout hole is completed, can form complete
Seepage proof curtain or reinforcing area, so as to realize the anti-seepage reinforcing to the thin silt that need to be processed.Specific construction parameter can
Chosen with the requirement with reference to cement grout.
Principle of the invention is explained with reference to embodiment and accompanying drawing.Experimental technique used in embodiment is such as without special
Illustrate, be conventional method.Material, reagent used etc., unless otherwise specified, are conventional material.
Embodiment 1
（1）Crept into projected depth using MD-50 rigs, to prevent collapse hole, using the follow-up retaining wall mode of protection casing 4；Aperture
108m, hole depth 15m；According to geological exploration data and actual borehole process analysis procedure analysis, this hole is Extra-fine sand ground in 6.5 ~ 14.5m of hole
Layer.Rig is removed after into grout hole 1, aperture protection is carried out, waits to fill.
（2）Toward two or more bacterium solution culture tanks 12（>1000L）Add microorganism fungus kind and the nutrient solution for preparing, mixing
Optimal conditions is adjusted to after uniform and conserves 24h, it is stand-by.
Microbial inoculum is made up of Bacillus pasteurii bacterium solution and nutrient solution, wherein, contain yeast per 1L nutrient solutions and carry
15~25g of thing is taken,（NH4）2SO4The Tris of 8~12g, 0.13mol, and calcium mixture liquid, regulation bacterium solution pH value are 6.24, training
Support 30 DEG C of temperature, inoculative proportion be 10% as microorganism optimal culture environment, accumulated with calcium liquid（Calcium ion molar concentration is
2.4mol/L）1.5 times for bacterium solution volume (bacterium solution OD600 values are between 1.5-2) are advisable.To reach enough consolidation effects,
The calcium carbonate quantity for asking microorganism to produce is enough and is evenly distributed.
（3）Toward calcium mixture source slot 11（>500L）The solution such as urea, calcium nitrate and calcium chloride are added in predetermined ratio, is mixed
Close uniform rear maintenance stand-by.
（4）Drawbench 5 is enclosed within and waits to fill on the protection casing 4 in drilling, by protection casing 4 using being pulled out on drawbench 5
5cm, is wrapped with closure mould bag 2 and blocks in the bottom of high pressure Grout-jetting pipe 6, the bottom of lower high pressure Grout-jetting pipe 6 to protection casing 4；Reserved
High-pressure grouting pipe 7 pours into the ratio of mud 2:1 cement slurry, until grouting pressure reaches 0.2MPa, continues 3min and terminates, using mould
The expansion of bag permeable impermeable slurry property mould bag, calculus, by the space closing between high pressure Grout-jetting pipe 6 and protection casing 4, prevent slurries
Gone here and there along sleeve pipe.
（5）The microbial inoculum that bacterium solution culture tank 12 is conserved injects microorganism slurries mixer by transfer pipeline 13
In 10, while mixing calcium source also is pressed into 1.5 by transfer pipeline 13 from calcium mixture source slot 11:1 ratio injection microorganism slurry
In liquid mixer 10, gate valve is opened after 2 ~ 3min of stirring at low speed, the grouting bucket 16 for allowing slurries to enter on high pressure chemistry grouting pump 9
In.
（6）After keeping return pipe 8 to be in opening, high pressure chemistry grouting pump 9 is opened, allow microorganism slurries to pass through high pressure
Grout pipe 7, high pressure Grout-jetting pipe 6 enter in stratum, while microorganism slurries are returned to also by return pipe 8 being in the milk in bucket 16.Using
High-pressure trend fills pump, and by high-pressure grouting pipe 7 and high pressure Grout-jetting pipe 6, directly pure pressure type irrigates microorganism slurries, its more stable grouting
Pressure and discharge capacity ensure that microorganism slurries are more uniform in the diffusion ratio of thin silt 3, fill full.
（7）Slowly closing return pipe 8, by adjusting the aperture of return pipe 8, the injection rate for controlling slurries is 2~10L/
Min, based on flow control, after certain injection rate is reached, grouting pressure will gradually rise, and reach the design grouting of setting
Pressure（Such as 3MPa）Afterwards, now should be constant by adjusting the aperture holding grouting pressure of return pipe 8, the injection rate of slurries will progressively
Reduce, until the injection rate of microorganism slurries reaches the ending standard of setting（Such as：0.5L/min）, you can terminate this section of perfusion.
（8）If the microorganism slurries amount in mixer is few, it is impossible to meet perfusion and require, perfusion can be stopped, according to step 5
Process is produced after microorganism slurries and is irrigated by 6,7 steps.Bacterium solution should not occur when a certain grout compartment is irrigated in principle
The not enough situation of slurries in culture tank 12 or/and calcium mixture source slot 11, if occurring, should interrupt perfusion.For anti-phenomenon here occurs,
The preparation of slurries should be carried out before perfusion, grout pouring is carried out if necessary micro- life is reduced with the space of filling Extra-fine sand as far as possible
The injection rate of thing slurries.
（9) after this section terminates grouting, high pressure chemistry grouting pump 9 is closed, protection casing 4 is used together with high pressure Grout-jetting pipe 6
Pulled out on drawbench 5, the situation of injection rate and injection rate according to the preceding paragraph, it is slow on pull out the supreme grout compartments of 5 ~ 50cm.
（10）5~8 process is repeated, this section of grouting is completed.If completing one saves protection casing, the length perfusion of about 1.5m
Afterwards, it is necessary to be unloaded using the protection casing 4 that drawbench 5 will have been extracted.
（11）Said process is repeated, until all grout compartments comprising thin silt 3 are fully completed the filling of microorganism slurries
Note.
（12）Other slurries such as cement can easily be changed carries out conventional perfusion to remaining grout compartment, its technological parameter
It is in the milk with reference to other slurries with process and is required, until this drilling has all been filled.
（13）After pending grout compartment all terminates, unloaded after rinsing high pressure Grout-jetting pipe 6, mixer, pump, pipeline are entered
Row cleaning.This hole is that can reach age requirement after 7 ~ 14d is coagulated.Next hole is moved to be irrigated.According to engine request, stratum feelings
The factor such as condition and perfusion situation carries out comprehensive analysis, and grouting pitch of holes control is in 2 ~ 3m.
Based on above-described embodiment, the invention provides a kind of cheap, environment-protecting asepsis and construction effect is reliable to powder
Fine sand stratum carries out the device and construction method of microorganism solidification.Compared with prior art, advantages of the present invention is protruded, to environment
Pollution it is small, the disturbance to the soil body is small, and microorganism grouting good penetrability, diffusion is uniform, and substantially, the soil body is in microorganism for consolidation effect
Under solidification, unconfined compressive strength is significantly improved.Compared with conventional grout material epoxy resin, acrylates, in antiseepage
In the case that reinforcement performance meets requirement, the influence to surrounding environment is almost 0, and construction cost can reduce by more than 40%.
The appearance of microorganism watertight grouting reinforcement technique, the development of the strong green grouting technique of promotion, also for environmental requirement is high, effect can
The basic fine sand layer anti-seepage reinforcing and reinforcement of concrete defect engineering for leaning on provide a kind of new means, can be widely used in water source
Engineering, reservoir, draw（It is defeated）In the engineerings such as water.
The present invention preferably specific embodiment is the foregoing is only, but protection scope of the present invention is not limited thereto,
Any one skilled in the art the invention discloses technical scope in the change or replacement expected, should all contain
Cover within protection scope of the present invention.
Claims (10)
1. a kind of device that microorganism solidification is carried out in thin silt, including bacterium solution culture tank（12）, microorganism slurries stirring
Machine（10）, calcium mixture source slot（11）, microorganism slurries mixer（10）By transfer pipeline（13）Respectively with bacterium solution culture tank
（12）, calcium mixture source slot（11）It is connected, it is characterised in that
Rig is utilized and crept into the bottom on pending stratum with managing and protecting wall pore-forming mode；
In protection casing（4）Bottom under high pressure Grout-jetting pipe（6）；
By in high pressure Grout-jetting pipe（6）The mode of pulp section grout sealing mould bag (2) obstruction closes protection casing（4）With injection high
Slurry pipe（6）Between space；
High pressure chemistry grouting pump（9）Microorganism slurries are passed through into high-pressure grouting pipe（7）With high pressure Grout-jetting pipe（6）Carry out pure pressure type filling
Note construction.
2. device according to claim 1, it is characterised in that the bacterium solution culture tank（12）Volume is not less than 1m3。
3. device according to claim 1, it is characterised in that the microorganism slurries mixer（10）Capacity is not less than
500L, mixing speed is less than 100r/min.
4. device according to claim 1, it is characterised in that the high pressure Grout-jetting pipe（6）With high-pressure grouting pipe（7）Adopt
High-pressure grouting pipe fitting, caliber is used to be not more than 50mm.
5. device according to claim 1, it is characterised in that the high pressure chemistry grouting pump（9）, irrigate microorganism slurries
When discharge capacity be 0~50L/min, pump intake pressure is 6MPa.
6. it is a kind of such as the construction method of claim 1-5 any one described devices, it is characterised in that to comprise the following steps：
Step 1）Rig creeps into thin silt（3）, using with manage and protect wall creep into the bottom on pending stratum pull out 0~
10cm, and in protection casing（4）Bottom under high pressure Grout-jetting pipe（6）；Hole inner sealing is carried out using form sack grouting, by injection high slurry
Pipe（6）And protection casing（4）Between space closing；
Step 2）Preparing microorganism slurries；
Step 3）Use high pressure chemistry grouting pump（9）By the step 2）Middle microorganism slurries pass through high pressure Grout-jetting pipe（6）Pump into choosing
Fixed grout compartment, in high pressure chemistry grouting pump（9）Under pressure effect, microorganism slurries are progressively spread, and grouting pressure is also progressively carried
The grouting pressure of setting is up to arrived, the injection rate of slurries will be gradually reduced, until reaching the injection rate of setting, this section can knot
Beam；
Step 4）By protection casing（4）Together with high pressure Grout-jetting pipe（6）Using drawbench（5）On pull out, according to the situation of injection rate, delay
The supreme grout compartments of 5~50cm are pulled out on slow；
Repeat step 3）~4）, until institute's pending processing thin silt（3）Grout compartment all completes the grouting of microorganism slurries；
Step 5）After pending grout compartment all terminates, high pressure Grout-jetting pipe is rinsed（6）After unload, then to mixer, pump, pipeline
Cleaned, this hole is that can reach age requirement after 7 ~ 14d is coagulated.
7. construction method according to claim 6, it is characterised in that the step 2）Including：
Step a, preparing microorganism bacterium solution, the microbial inoculum is made up of Bacillus pasteurii and nutrient solution, wherein, per 1L
Nutrient solution contains 15~25g of yeast extract,（NH4）2SO4The Tris of 8~12g, 0.13mol, and calcium mixture liquid, adjust bacterium
Liquid pH value is 6.24, to adapt to Bacillus pasteurii growth；Wherein, calcium mixture liquid is made up of urea, calcium nitrate and calcium chloride, rubs
You are than being 2:1:1；
Step b, 24h is cultivated by Bacillus pasteurii in nutrient solution；
Step c, by the microorganism mixed liquor in step b in microorganism slurries mixer（10）2~3min of middle mixing.
8. construction method according to claim 7, it is characterised in that calcium mixture liquid product and Pasteur's bud in the step a
Spore bacillus bacterium solution volume ratio is 1.5~2:1.
9. construction method according to claim 7, it is characterised in that the inoculative proportion of the Bacillus pasteurii is 10%,
28 DEG C~35 DEG C of cultivation temperature.
10. construction method according to claim 7, it is characterised in that described in thin silt（3）In carry out microorganism
The method of solidification is using the grout construction method from bottom to top, being progressively segmented.
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