CN109182415A - It is a kind of with the water-disintegrable polysaccharide and preparation method of lower blood-fat and reduce weight function and application - Google Patents
It is a kind of with the water-disintegrable polysaccharide and preparation method of lower blood-fat and reduce weight function and application Download PDFInfo
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- CN109182415A CN109182415A CN201810905688.6A CN201810905688A CN109182415A CN 109182415 A CN109182415 A CN 109182415A CN 201810905688 A CN201810905688 A CN 201810905688A CN 109182415 A CN109182415 A CN 109182415A
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- water
- fat
- lower blood
- reduce weight
- polysaccharide
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- 150000004676 glycans Chemical class 0.000 title claims abstract description 62
- 229920001282 polysaccharide Polymers 0.000 title claims abstract description 62
- 239000005017 polysaccharide Substances 0.000 title claims abstract description 62
- 238000002360 preparation method Methods 0.000 title claims abstract description 44
- 150000001720 carbohydrates Chemical class 0.000 claims abstract description 66
- 235000014633 carbohydrates Nutrition 0.000 claims abstract description 66
- 230000007071 enzymatic hydrolysis Effects 0.000 claims abstract description 40
- 238000006047 enzymatic hydrolysis reaction Methods 0.000 claims abstract description 40
- 239000007864 aqueous solution Substances 0.000 claims abstract description 21
- 235000004426 flaxseed Nutrition 0.000 claims description 46
- DBTMGCOVALSLOR-DEVYUCJPSA-N (2s,3r,4s,5r,6r)-4-[(2s,3r,4s,5r,6r)-3,5-dihydroxy-6-(hydroxymethyl)-4-[(2s,3r,4s,5s,6r)-3,4,5-trihydroxy-6-(hydroxymethyl)oxan-2-yl]oxyoxan-2-yl]oxy-6-(hydroxymethyl)oxane-2,3,5-triol Chemical compound O[C@@H]1[C@@H](O)[C@H](O)[C@@H](CO)O[C@H]1O[C@@H]1[C@@H](O)[C@H](O[C@H]2[C@@H]([C@@H](CO)O[C@H](O)[C@@H]2O)O)O[C@H](CO)[C@H]1O DBTMGCOVALSLOR-DEVYUCJPSA-N 0.000 claims description 29
- 229920001543 Laminarin Polymers 0.000 claims description 29
- 239000005717 Laminarin Substances 0.000 claims description 29
- 229920002752 Konjac Polymers 0.000 claims description 26
- 235000010485 konjac Nutrition 0.000 claims description 25
- 235000001206 Amorphophallus rivieri Nutrition 0.000 claims description 23
- 239000000252 konjac Substances 0.000 claims description 23
- MJYQFWSXKFLTAY-OVEQLNGDSA-N (2r,3r)-2,3-bis[(4-hydroxy-3-methoxyphenyl)methyl]butane-1,4-diol;(2r,3r,4s,5s,6r)-6-(hydroxymethyl)oxane-2,3,4,5-tetrol Chemical compound OC[C@H]1O[C@@H](O)[C@H](O)[C@@H](O)[C@@H]1O.C1=C(O)C(OC)=CC(C[C@@H](CO)[C@H](CO)CC=2C=C(OC)C(O)=CC=2)=C1 MJYQFWSXKFLTAY-OVEQLNGDSA-N 0.000 claims description 22
- 108010059892 Cellulase Proteins 0.000 claims description 17
- 108090000790 Enzymes Proteins 0.000 claims description 14
- 102000004190 Enzymes Human genes 0.000 claims description 14
- 230000000694 effects Effects 0.000 claims description 14
- 235000015110 jellies Nutrition 0.000 claims description 13
- 239000008274 jelly Substances 0.000 claims description 13
- 101710130619 Glucan endo-1,3-beta-glucosidase Proteins 0.000 claims description 9
- 101710184061 Glucan endo-1,3-beta-glucosidase, acidic isoform Proteins 0.000 claims description 9
- 101710180749 Glucan endo-1,3-beta-glucosidase, basic isoform Proteins 0.000 claims description 9
- 102100032487 Beta-mannosidase Human genes 0.000 claims description 8
- 108010055059 beta-Mannosidase Proteins 0.000 claims description 8
- 235000013305 food Nutrition 0.000 claims description 6
- 235000013361 beverage Nutrition 0.000 claims description 5
- 235000012054 meals Nutrition 0.000 claims description 5
- 239000007787 solid Substances 0.000 claims description 5
- 235000013376 functional food Nutrition 0.000 claims description 4
- 239000000843 powder Substances 0.000 claims description 4
- 235000013618 yogurt Nutrition 0.000 claims description 4
- -1 drink Substances 0.000 claims description 3
- LUEWUZLMQUOBSB-FSKGGBMCSA-N (2s,3s,4s,5s,6r)-2-[(2r,3s,4r,5r,6s)-6-[(2r,3s,4r,5s,6s)-4,5-dihydroxy-2-(hydroxymethyl)-6-[(2r,4r,5s,6r)-4,5,6-trihydroxy-2-(hydroxymethyl)oxan-3-yl]oxyoxan-3-yl]oxy-4,5-dihydroxy-2-(hydroxymethyl)oxan-3-yl]oxy-6-(hydroxymethyl)oxane-3,4,5-triol Chemical compound O[C@H]1[C@@H](O)[C@H](O)[C@@H](CO)O[C@H]1O[C@@H]1[C@@H](CO)O[C@@H](O[C@@H]2[C@H](O[C@@H](OC3[C@H](O[C@@H](O)[C@@H](O)[C@H]3O)CO)[C@@H](O)[C@H]2O)CO)[C@H](O)[C@H]1O LUEWUZLMQUOBSB-FSKGGBMCSA-N 0.000 claims description 2
- 229920002581 Glucomannan Polymers 0.000 claims description 2
- 229940046240 glucomannan Drugs 0.000 claims description 2
- 241001312219 Amorphophallus konjac Species 0.000 claims 2
- JEIPFZHSYJVQDO-UHFFFAOYSA-N iron(III) oxide Inorganic materials O=[Fe]O[Fe]=O JEIPFZHSYJVQDO-UHFFFAOYSA-N 0.000 claims 1
- 230000000968 intestinal effect Effects 0.000 abstract description 14
- 238000012423 maintenance Methods 0.000 abstract description 2
- 229920001542 oligosaccharide Polymers 0.000 abstract 1
- 150000002482 oligosaccharides Chemical class 0.000 abstract 1
- KRKNYBCHXYNGOX-UHFFFAOYSA-N citric acid Chemical compound OC(=O)CC(O)(C(O)=O)CC(O)=O KRKNYBCHXYNGOX-UHFFFAOYSA-N 0.000 description 93
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 73
- 239000000413 hydrolysate Substances 0.000 description 35
- UEDUENGHJMELGK-HYDKPPNVSA-N Stevioside Chemical compound O([C@@H]1[C@@H](O)[C@H](O)[C@@H](CO)O[C@H]1O[C@]12C(=C)C[C@@]3(C1)CC[C@@H]1[C@@](C)(CCC[C@]1([C@@H]3CC2)C)C(=O)O[C@H]1[C@@H]([C@@H](O)[C@H](O)[C@@H](CO)O1)O)[C@@H]1O[C@H](CO)[C@@H](O)[C@H](O)[C@H]1O UEDUENGHJMELGK-HYDKPPNVSA-N 0.000 description 31
- 229940013618 stevioside Drugs 0.000 description 31
- OHHNJQXIOPOJSC-UHFFFAOYSA-N stevioside Natural products CC1(CCCC2(C)C3(C)CCC4(CC3(CCC12C)CC4=C)OC5OC(CO)C(O)C(O)C5OC6OC(CO)C(O)C(O)C6O)C(=O)OC7OC(CO)C(O)C(O)C7O OHHNJQXIOPOJSC-UHFFFAOYSA-N 0.000 description 31
- 235000019202 steviosides Nutrition 0.000 description 31
- 240000006240 Linum usitatissimum Species 0.000 description 24
- 235000004431 Linum usitatissimum Nutrition 0.000 description 24
- 244000247812 Amorphophallus rivieri Species 0.000 description 21
- 239000000049 pigment Substances 0.000 description 21
- 238000003756 stirring Methods 0.000 description 19
- 239000012153 distilled water Substances 0.000 description 18
- 239000004615 ingredient Substances 0.000 description 14
- 201000006549 dyspepsia Diseases 0.000 description 13
- 238000001514 detection method Methods 0.000 description 12
- 238000002156 mixing Methods 0.000 description 12
- 238000010010 raising Methods 0.000 description 12
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 description 11
- 239000008103 glucose Substances 0.000 description 11
- CHHHXKFHOYLYRE-UHFFFAOYSA-M 2,4-Hexadienoic acid, potassium salt (1:1), (2E,4E)- Chemical compound [K+].CC=CC=CC([O-])=O CHHHXKFHOYLYRE-UHFFFAOYSA-M 0.000 description 10
- 239000000499 gel Substances 0.000 description 10
- 235000010241 potassium sorbate Nutrition 0.000 description 10
- 239000004302 potassium sorbate Substances 0.000 description 10
- 229940069338 potassium sorbate Drugs 0.000 description 10
- 150000001875 compounds Chemical class 0.000 description 9
- 229940088598 enzyme Drugs 0.000 description 9
- 229940106157 cellulase Drugs 0.000 description 8
- 239000000203 mixture Substances 0.000 description 8
- 239000000047 product Substances 0.000 description 8
- WXMKPNITSTVMEF-UHFFFAOYSA-M sodium benzoate Chemical compound [Na+].[O-]C(=O)C1=CC=CC=C1 WXMKPNITSTVMEF-UHFFFAOYSA-M 0.000 description 8
- 235000010234 sodium benzoate Nutrition 0.000 description 8
- 239000004299 sodium benzoate Substances 0.000 description 8
- 238000012360 testing method Methods 0.000 description 8
- 231100000252 nontoxic Toxicity 0.000 description 7
- 230000003000 nontoxic effect Effects 0.000 description 7
- 239000000243 solution Substances 0.000 description 7
- 230000001954 sterilising effect Effects 0.000 description 7
- 241000206575 Chondrus crispus Species 0.000 description 5
- 230000007062 hydrolysis Effects 0.000 description 5
- 238000006460 hydrolysis reaction Methods 0.000 description 5
- 230000003020 moisturizing effect Effects 0.000 description 5
- 239000002574 poison Substances 0.000 description 5
- 231100000614 poison Toxicity 0.000 description 5
- 229920001285 xanthan gum Polymers 0.000 description 5
- 239000000230 xanthan gum Substances 0.000 description 5
- 229940082509 xanthan gum Drugs 0.000 description 5
- 235000010493 xanthan gum Nutrition 0.000 description 5
- 235000013325 dietary fiber Nutrition 0.000 description 4
- 238000002474 experimental method Methods 0.000 description 4
- 229960004106 citric acid Drugs 0.000 description 3
- 235000015165 citric acid Nutrition 0.000 description 3
- 235000005911 diet Nutrition 0.000 description 3
- 230000037213 diet Effects 0.000 description 3
- 229960003885 sodium benzoate Drugs 0.000 description 3
- 210000001072 colon Anatomy 0.000 description 2
- 230000003111 delayed effect Effects 0.000 description 2
- 239000000835 fiber Substances 0.000 description 2
- 239000003292 glue Substances 0.000 description 2
- 230000036541 health Effects 0.000 description 2
- 238000000034 method Methods 0.000 description 2
- 235000019629 palatability Nutrition 0.000 description 2
- IZPNTDOWOBGHEG-UHFFFAOYSA-M sodium benzene formate Chemical compound [Na+].[O-]C=O.C1=CC=CC=C1 IZPNTDOWOBGHEG-UHFFFAOYSA-M 0.000 description 2
- QTBSBXVTEAMEQO-UHFFFAOYSA-M Acetate Chemical compound CC([O-])=O QTBSBXVTEAMEQO-UHFFFAOYSA-M 0.000 description 1
- 241000894006 Bacteria Species 0.000 description 1
- FERIUCNNQQJTOY-UHFFFAOYSA-M Butyrate Chemical compound CCCC([O-])=O FERIUCNNQQJTOY-UHFFFAOYSA-M 0.000 description 1
- FERIUCNNQQJTOY-UHFFFAOYSA-N Butyric acid Natural products CCCC(O)=O FERIUCNNQQJTOY-UHFFFAOYSA-N 0.000 description 1
- 241000196324 Embryophyta Species 0.000 description 1
- 208000008589 Obesity Diseases 0.000 description 1
- XBDQKXXYIPTUBI-UHFFFAOYSA-M Propionate Chemical compound CCC([O-])=O XBDQKXXYIPTUBI-UHFFFAOYSA-M 0.000 description 1
- 240000008042 Zea mays Species 0.000 description 1
- 235000005824 Zea mays ssp. parviglumis Nutrition 0.000 description 1
- 235000002017 Zea mays subsp mays Nutrition 0.000 description 1
- GZCGUPFRVQAUEE-SLPGGIOYSA-N aldehydo-D-glucose Chemical compound OC[C@@H](O)[C@@H](O)[C@H](O)[C@@H](O)C=O GZCGUPFRVQAUEE-SLPGGIOYSA-N 0.000 description 1
- 230000008901 benefit Effects 0.000 description 1
- 238000009835 boiling Methods 0.000 description 1
- 239000002131 composite material Substances 0.000 description 1
- 238000013329 compounding Methods 0.000 description 1
- 235000005822 corn Nutrition 0.000 description 1
- 230000007812 deficiency Effects 0.000 description 1
- 238000004090 dissolution Methods 0.000 description 1
- 238000004821 distillation Methods 0.000 description 1
- 238000000855 fermentation Methods 0.000 description 1
- 230000004151 fermentation Effects 0.000 description 1
- 239000012467 final product Substances 0.000 description 1
- PCHJSUWPFVWCPO-UHFFFAOYSA-N gold Chemical compound [Au] PCHJSUWPFVWCPO-UHFFFAOYSA-N 0.000 description 1
- 239000010931 gold Substances 0.000 description 1
- 229910052737 gold Inorganic materials 0.000 description 1
- 230000003301 hydrolyzing effect Effects 0.000 description 1
- 230000031891 intestinal absorption Effects 0.000 description 1
- 238000011835 investigation Methods 0.000 description 1
- 229920005610 lignin Polymers 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 235000020824 obesity Nutrition 0.000 description 1
- 230000008569 process Effects 0.000 description 1
- 238000012545 processing Methods 0.000 description 1
- 230000036186 satiety Effects 0.000 description 1
- 235000019627 satiety Nutrition 0.000 description 1
- 210000000813 small intestine Anatomy 0.000 description 1
- 238000006467 substitution reaction Methods 0.000 description 1
Classifications
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P19/00—Preparation of compounds containing saccharide radicals
- C12P19/14—Preparation of compounds containing saccharide radicals produced by the action of a carbohydrase (EC 3.2.x), e.g. by alpha-amylase, e.g. by cellulase, hemicellulase
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23C—DAIRY PRODUCTS, e.g. MILK, BUTTER OR CHEESE; MILK OR CHEESE SUBSTITUTES; MAKING THEREOF
- A23C9/00—Milk preparations; Milk powder or milk powder preparations
- A23C9/12—Fermented milk preparations; Treatment using microorganisms or enzymes
- A23C9/13—Fermented milk preparations; Treatment using microorganisms or enzymes using additives
- A23C9/1307—Milk products or derivatives; Fruit or vegetable juices; Sugars, sugar alcohols, sweeteners; Oligosaccharides; Organic acids or salts thereof or acidifying agents; Flavours, dyes or pigments; Inert or aerosol gases; Carbonation methods
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L2/00—Non-alcoholic beverages; Dry compositions or concentrates therefor; Their preparation
- A23L2/385—Concentrates of non-alcoholic beverages
- A23L2/39—Dry compositions
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L21/00—Marmalades, jams, jellies or the like; Products from apiculture; Preparation or treatment thereof
- A23L21/10—Marmalades; Jams; Jellies; Other similar fruit or vegetable compositions; Simulated fruit products
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L29/00—Foods or foodstuffs containing additives; Preparation or treatment thereof
- A23L29/30—Foods or foodstuffs containing additives; Preparation or treatment thereof containing carbohydrate syrups; containing sugars; containing sugar alcohols, e.g. xylitol; containing starch hydrolysates, e.g. dextrin
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
- A23L33/10—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
- A23L33/125—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives containing carbohydrate syrups; containing sugars; containing sugar alcohols; containing starch hydrolysates
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P19/00—Preparation of compounds containing saccharide radicals
- C12P19/04—Polysaccharides, i.e. compounds containing more than five saccharide radicals attached to each other by glycosidic bonds
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2002/00—Food compositions, function of food ingredients or processes for food or foodstuffs
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Abstract
The invention discloses a kind of with the water-disintegrable polysaccharide and preparation method of lower blood-fat and reduce weight function and application.For the present invention by digesting to indigestible carbohydrates, enzymatic hydrolysis is all dissolved into aqueous solution to indigestible carbohydrates, obtains the water-disintegrable polysaccharide with lower blood-fat and reduce weight function.The preparation method is simple, and obtained water-disintegrable polysaccharide is not easy to be absorbed by the body, and contains oligosaccharide, to have the function of that lower blood-fat and reduce weight, maintenance intestinal flora are in a healthy and balanced way.
Description
Technical field
The invention belongs to biological food health product technology fields, and in particular to a kind of with the water-disintegrable of lower blood-fat and reduce weight function
Polysaccharide and preparation method and application.
Background technique
Dietary fiber is mainly the indigestible carbohydrate and lignin from plant, they cannot be in small intestine
It is degraded and absorbed, therefore is not likely to produce energy matter and is easy to produce satiety, therefore, the public generally believes that intake diet is fine
Dimension has the effect of weight-reducing.But in fact really not so, many dietary fibers are not in small intestinal absorption, but in the colon of people by colon bacterium
Fermentation generates energy matter such as acetate, propionate and butyrate etc., therefore, not all indigestible carbohydrates
With fat-reducing effect, but the food for taking in the fiber containing specific meal just has the function of that weight is inhibited even to reduce weight.One
The investigation of a little epidemiology is also shown, and part dietary fiber is especially full paddy or the intake of corn fiber can inhibit obesity really
It is formed.But due to the palatability problems of high dietary-fiber diet, and it is not affected by being widely recognized as or receiving for society.
Summary of the invention
In order to overcome the disadvantages and deficiencies of the prior art, the primary purpose of the present invention is that providing a kind of with lower blood-fat and reduce weight
The preparation method of the water-disintegrable polysaccharide of function.The water-disintegrable polysaccharide with lower blood-fat and reduce weight function is by indigestion carbon water
Compound is digested to obtain.
Another object of the present invention is to provide the hydrolysis with lower blood-fat and reduce weight function obtained by above-mentioned preparation method
Property polysaccharide.
Another object of the present invention is to provide the applications of the above-mentioned water-disintegrable polysaccharide with lower blood-fat and reduce weight function.
Goal of the invention of the invention is achieved through the following technical solutions: a kind of water-disintegrable polysaccharide with lower blood-fat and reduce weight function
Preparation method, include the following steps: to digest indigestible carbohydrates, enzymatic hydrolysis is to indigestible carbohydrates
It all is dissolved into aqueous solution, obtains the water-disintegrable polysaccharide with lower blood-fat and reduce weight function.
The indigestible carbohydrates be preferably in flaxseed gum, konjak glucomannan and laminarin extremely
Few one kind；More preferably flaxseed gum.
The enzyme be preferably in mannonase β -1,4- endoglucanase and β -1,3- endoglucanase extremely
Few one kind；More preferably β -1,4- endoglucanase.
The indigestible carbohydrates and the enzyme are preferably the proportion of 40~120:1 in mass ratio；More preferably
For 50~100:1 in mass ratio.
The temperature of the enzymatic hydrolysis is preferably 35~45 DEG C；More preferably 40 DEG C.
The enzymatic hydrolysis is all dissolved to indigestible carbohydrates, and degree of hydrolysis at this time is at least 40%, preferably
40~60%.
When the indigestible carbohydrates are flaxseed gum, the enzyme is preferably β-Isosorbide-5-Nitrae-endo-glucanase
Enzyme.
In enzymatic hydrolysis, the flaxseed gum and the β-Isosorbide-5-Nitrae-endoglucanase be preferably in mass ratio 40~
60:1 proportion；More preferably in mass ratio 50:1 proportion.
When the indigestible carbohydrates are laminarin, the enzyme is preferably β -1,3- endo-glucanase
Enzyme.
In enzymatic hydrolysis, the laminarin and the β -1,3- endoglucanase preferably in mass ratio 60~
90:1 proportion；More preferably in mass ratio 75~80:1 proportion.
When the indigestible carbohydrates are konjac polysaccharide, the enzyme is preferably mannase.
In enzymatic hydrolysis, the konjac polysaccharide and the mannase are preferably the proportion of 80~120:1 in mass ratio；
More preferably in mass ratio 100:1 proportion.
A kind of water-disintegrable polysaccharide with lower blood-fat and reduce weight function, is obtained by above-mentioned preparation method.
The water-disintegrable polysaccharide with lower blood-fat and reduce weight function has the application in lower blood-fat and reduce weight functional food in preparation.
The application is used alone including the different Polysaccharides with lower blood-fat and reduce weight function and Application of composite.
The food includes solid beverage, meal replacement powder, drink, jelly, Yoghourt etc..
It is a kind of to have effects that the drink of lower blood-fat and reduce weight, contain the above-mentioned water-disintegrable polysaccharide with lower blood-fat and reduce weight function.
It is a kind of to have effects that the jelly of lower blood-fat and reduce weight, contain the above-mentioned water-disintegrable polysaccharide with lower blood-fat and reduce weight function.
It is a kind of to have effects that the Yoghourt of lower blood-fat and reduce weight, contain the above-mentioned water-disintegrable polysaccharide with lower blood-fat and reduce weight function.
It is a kind of to have effects that the solid beverage of lower blood-fat and reduce weight, contain the above-mentioned water-disintegrable polysaccharide with lower blood-fat and reduce weight function.
It is a kind of to have effects that the meal replacement powder of lower blood-fat and reduce weight, contain the above-mentioned water-disintegrable polysaccharide with lower blood-fat and reduce weight function.
The present invention has the advantage that and effect:
(1) many indigestible carbohydrates, which are reported, has the function of lower blood-fat and reduce weight, but not readily dissolve, can only
The disadvantage being added in food in solid form, main reason is that these indigestible carbohydrates are dissolved in the water
When, (such as 1% even following) just forms gel at low concentrations, cannot be prepared into beverage；Some can be used for preparing jelly,
But the content in jelly is lower (such as 1% or so), and the amount daily intaked is caused to be not enough to reach lower blood-fat and reduce weight effect.This hair
It is bright that target carbohydrate is digested in advance using enzymolysis process, to increase its water solubility.Therefore, the polysaccharide after hydrolyzing
It can make final product that there is the carbohydrate of high-content.
(2) present invention digests indigestible carbohydrates using specific enzyme, and obtained Polysaccharides are not allowed
The characteristics of being easily absorbed by the body, remain indigestible carbohydrates.
(3) present invention carries out lower blood-fat and reduce weight using the enzymolysis product of insoluble carbohydrate, while having both maintenance
Intestinal flora function in a healthy and balanced way avoids the shortcomings that existing part diet products impair one's health.
(4) the present invention is based on the preparation method of the lower blood-fat and reduce weight functional food of indigestible carbohydrates is simple, processing
Cost is relatively low, but product has both functional, nature of leisure and palatability, added value are very high.
Specific embodiment
Below with reference to embodiment, the present invention is described in further detail, and embodiments of the present invention are not limited thereto.
Enzyme source used in the present invention is as follows:
β -1,4- endoglucanase and β -1,3- endoglucanase derive from Megazyme company of the U.S..
The cellulase and mannase of food system derive from Jiangsu Province gold riverhead bioengineering Co., Ltd.
Embodiment 1
(1) preparation of indigestion carbohydrate enzymatic hydrolysis object:
It accurately weighs 0.1g β-Isosorbide-5-Nitrae-endoglucanase to be dissolved in 100mL distilled water, it is (sub- to weigh 5g Linseed pigment
Numb seed glue), it is slowly added in water, 40 DEG C of water-baths simultaneously stir enzymatic hydrolysis, until Linseed pigment is all dissolved into aqueous solution, obtain Asia
Numb seed polysaccharide hydrolysate A；
It accurately weighs 0.1g cellulase to be dissolved in 100mL distilled water, weighs 5g Linseed pigment (flaxseed gum), delay
Slowly it makes an addition in water, 40 DEG C of water-baths simultaneously stir enzymatic hydrolysis, until Linseed pigment is all dissolved into aqueous solution, obtain Linseed pigment water
Solve object B.
(2) preparation of the drink based on indigestible carbohydrates: taking flaxseed gum hydrolysate made from step (1),
It gradually adds stevioside and citric acid mixing is dissolved in the water, bottle, 121 DEG C of sterilizing at least 15min；Be subsequently cooled to 40 DEG C with
Under, obtain the drink based on indigestible carbohydrates, wherein the mass percent of each component in drink are as follows: linseed is more
Sugared hydrolysate A or B 5%, stevioside 0.4%, citric acid 1.5%, water supplies 100%, respectively obtains drink A and drink B；
Control group is set simultaneously, the composition of control group is as follows: glucose 5%, stevioside 0.4%, citric acid 1.5%, water
Supply 100%.
(3) efficacy detection: it is divided into tri- groups of A, B, C using 30 25~30 years old male volunteers and carries out double blind reality as object
It tests, every group 10.Wherein, A group is taken in control group 100mL/ days, and B group takes in drink B 100mL/ days；C group takes in drink A
100mL/ days.It is consecutively ingested 30 days, detects weight, significantly reduce 5.1% before C group weight is not drunk as the result is shown, and A
Weight then all raisings of group and B group.
Drink A provided in this embodiment is the lower blood-fat and reduce weight drink with function based on indigestible carbohydrates, natural
It is nontoxic, there are multiple functions, for example maintain intestinal flora in a healthy and balanced way etc..
Embodiment 2
(1) preparation of indigestion carbohydrate enzymatic hydrolysis object:
It accurately weighs 0.2g β-Isosorbide-5-Nitrae-endoglucanase to be dissolved in 100mL distilled water, weighs 10g Linseed pigment
(flaxseed gum), is slowly added in water, and 40 DEG C of water-baths simultaneously stir enzymatic hydrolysis, until Linseed pigment is all dissolved into aqueous solution, obtains
To Linseed pigment hydrolysate A；
It accurately weighs 0.2g cellulase to be dissolved in 100mL distilled water, weighs 10g Linseed pigment (flaxseed gum),
It is slowly added in water, 40 DEG C of water-baths simultaneously stir enzymatic hydrolysis, until Linseed pigment is all dissolved into aqueous solution, obtain Linseed pigment
Hydrolysate B.
(2) preparation of the drink based on indigestible carbohydrates: taking flaxseed gum hydrolysate made from step (1),
It gradually adds stevioside and citric acid mixing is dissolved in the water, bottle, 121 DEG C of sterilizing at least 15min；Be subsequently cooled to 40 DEG C with
Under, obtain the drink based on indigestible carbohydrates, wherein the mass percent of each component in drink are as follows: linseed is more
Sugared hydrolysate A or B 10%, stevioside 0.3%, citric acid 2.0%, water supplies 100%, respectively obtains drink A and drink B；
Control group is set simultaneously, the composition of control group is as follows: glucose 10%, stevioside 0.3%, citric acid 2.0%, water
Supply 100%.
(3) efficacy detection: it is divided into tri- groups of A, B, C using 30 25~30 years old male volunteers and carries out double blind reality as object
It tests, every group 10.Wherein, A group is taken in control group 100mL/ days, and B group takes in drink B 100mL/ days；C group takes in drink A
100mL/ days.It is consecutively ingested 30 days, detects weight, significantly reduce 5.7% before C group weight is not drunk as the result is shown, and A
Group and all raisings of B group weight.
Drink A provided in this embodiment is the lower blood-fat and reduce weight drink with function based on indigestible carbohydrates, natural
It is nontoxic, there are multiple functions, for example maintain intestinal flora in a healthy and balanced way etc..
Embodiment 3
(1) preparation of indigestion carbohydrate enzymatic hydrolysis object:
It accurately weighs 0.4g β-Isosorbide-5-Nitrae-endoglucanase to be dissolved in 100mL distilled water, weighs 20g Linseed pigment
(flaxseed gum), is slowly added in water, and 40 DEG C of water-baths simultaneously stir enzymatic hydrolysis, until Linseed pigment is all dissolved into aqueous solution, obtains
To Linseed pigment hydrolysate A；
It accurately weighs 0.4g cellulase to be dissolved in 100mL distilled water, weighs 20g Linseed pigment (flaxseed gum),
It is slowly added in water, 40 DEG C of water-baths simultaneously stir enzymatic hydrolysis, until Linseed pigment is all dissolved into aqueous solution, obtain Linseed pigment
Hydrolysate B.
(2) preparation of the drink based on indigestible carbohydrates: taking flaxseed gum hydrolysate made from step (1),
It gradually adds stevioside and citric acid mixing is dissolved in the water, bottle, 121 DEG C of sterilizing at least 15min；Be subsequently cooled to 40 DEG C with
Under, obtain the drink based on indigestible carbohydrates, wherein the mass percent of each component in drink are as follows: linseed is more
Sugared hydrolysate A or B 20%, stevioside 0.1%, citric acid 2.5%, water supplies 100%, respectively obtains drink A and drink B；
Control group is set simultaneously, the composition of control group is as follows: glucose 20%, stevioside 0.1%, citric acid 2.5%, water
Supply 100%.
(3) efficacy detection: it is divided into tri- groups of A, B, C using 30 25~30 years old male volunteers and carries out double blind reality as object
It tests, every group 10.Wherein, A group is taken in control group 100mL/ days, and B group takes in drink B 100mL/ days；C group takes in drink A
100mL/ days.It is consecutively ingested 30 days, detects weight, significantly reduce 6.3% before C group weight is not drunk as the result is shown, and A
With the then all raisings of B group weight.
Lower blood-fat and reduce weight drink with function of the drink A provided in this embodiment for indigestible carbohydrates, Nantural non-toxic,
With multiple functions, for example maintain intestinal flora in a healthy and balanced way etc..
Embodiment 4
(1) preparation of indigestion carbohydrate enzymatic hydrolysis object:
0.4g β -1 is accurately weighed, 3- endoglucanase is dissolved in 100mL distilled water, weighs 30g laminarin, is delayed
Slowly it makes an addition in water, 40 DEG C of water-baths simultaneously stir enzymatic hydrolysis, until laminarin is all dissolved into aqueous solution, obtain laminarin hydrolysate
A；
It accurately weighs 0.4g cellulase to be dissolved in 100mL distilled water, weighs 30g laminarin, be slowly added in water
In, 40 DEG C of water-baths simultaneously stir enzymatic hydrolysis, until laminarin is all dissolved into aqueous solution, obtain laminarin hydrolysate B.
(2) preparation of the drink based on indigestible carbohydrates: taking laminarin hydrolysate made from step (1),
It gradually adds stevioside and citric acid mixing is dissolved in the water, bottle, 121 DEG C of sterilizing at least 15min；Be subsequently cooled to 40 DEG C with
Under, obtain the drink based on indigestible carbohydrates, wherein the mass percent of each component in drink are as follows: laminarin
Hydrolysate A or B 30%, stevioside 0.1%, citric acid 3%, water supplies 100%, respectively obtains drink A and drink B；
Control group is set simultaneously, the composition of control group is as follows: glucose 30%, stevioside 0.1%, citric acid 3%, and water is mended
Foot 100%.
(3) efficacy detection: it is divided into tri- groups of A, B, C using 30 25~30 years old male volunteers and carries out double blind reality as object
It tests, every group 10.Wherein, A group is taken in control group 100mL/ days, and B group takes in drink B 100mL/ days；C group takes in drink A
100mL/ days.It is consecutively ingested 30 days, detects weight, significantly reduce 10.3% before C group weight is not drunk as the result is shown, and
The then all raisings of A and B group weight.
Drink A provided in this embodiment is lower blood-fat and reduce weight drink with function, and Nantural non-toxic has multiple functions, for example ties up
It is in a healthy and balanced way etc. to hold intestinal flora.
Embodiment 5
(1) preparation of indigestion carbohydrate enzymatic hydrolysis object:
0.5g β -1 is accurately weighed, 3- endoglucanase is dissolved in 100mL distilled water, weighs 40g laminarin, is delayed
Slowly it makes an addition in water, 40 DEG C of water-baths simultaneously stir enzymatic hydrolysis, until laminarin is all dissolved into aqueous solution, obtain laminarin hydrolysis
Object；
It accurately weighs 0.5g cellulase to be dissolved in 100mL distilled water, weighs 40g laminarin, be slowly added in water
In, 40 DEG C of water-baths simultaneously stir enzymatic hydrolysis, until laminarin is all dissolved into aqueous solution, obtain laminarin hydrolysate.
(2) preparation of the drink based on indigestible carbohydrates: taking laminarin hydrolysate made from step (1),
It gradually adds stevioside and citric acid mixing is dissolved in the water, bottle, 121 DEG C of sterilizing at least 15min；Be subsequently cooled to 40 DEG C with
Under, obtain the drink based on indigestible carbohydrates, wherein the mass percent of each component in drink are as follows: laminarin
Hydrolysate A or B 40%, stevioside 0.1%, citric acid 4%, water supplies 100%, respectively obtains drink A and drink B；
Control group is set simultaneously, the composition of control group is as follows: glucose 40%, stevioside 0.1%, citric acid 4%, and water is mended
Foot 100%.
(3) efficacy detection: it is divided into tri- groups of A, B, C using 30 25~30 years old male volunteers and carries out double blind reality as object
It tests, every group 10.Wherein, A group is taken in control group 100mL/ days, and B group takes in drink B 100mL/ days；C group takes in drink A
100mL/ days.It is consecutively ingested 30 days, detects weight, significantly reduce 10.8% before C group weight is not drunk as the result is shown, and
The then all raisings of A and B group weight.
Drink A provided in this embodiment is the lower blood-fat and reduce weight drink with function based on indigestible carbohydrates, natural
It is nontoxic, there are multiple functions, for example maintain intestinal flora in a healthy and balanced way etc..
Embodiment 6
(1) preparation of indigestion carbohydrate enzymatic hydrolysis object:
Accurately weigh 0.3g mannase to be dissolved in 100mL distilled water, weigh 30g konjac polysaccharide, be slowly added in
In water, 40 DEG C of water-baths simultaneously stir enzymatic hydrolysis, until konjac polysaccharide is all dissolved into aqueous solution, obtain konjac polysaccharide hydrolysate A；
It accurately weighs 0.3g cellulase to be dissolved in 100mL distilled water, weighs 30g konjac polysaccharide, be slowly added in water
In, 40 DEG C of water-baths simultaneously stir enzymatic hydrolysis, until konjac polysaccharide is all dissolved into aqueous solution, obtain konjac polysaccharide hydrolysate B.
(2) preparation of the drink based on indigestible carbohydrates: taking konjac polysaccharide hydrolysate made from step (1),
It gradually adds stevioside and citric acid mixing is dissolved in the water, bottle, 121 DEG C of sterilizing at least 15min；Be subsequently cooled to 40 DEG C with
Under, obtain the drink based on indigestible carbohydrates, wherein the mass percent of each component in drink are as follows: konjac polysaccharide
Hydrolysate A or B 25%, stevioside 0.1%, citric acid 3%, water supplies 100%, respectively obtains drink A and drink B；
Control group is set simultaneously, the composition of control group is as follows: glucose A or B 25%, stevioside 0.1%, citric acid
3%, water supplies 100%.
(3) efficacy detection: it is divided into tri- groups of A, B, C using 30 25~30 years old male volunteers and carries out double blind reality as object
It tests, every group 10.Wherein, A group is taken in control group 100mL/ days, and B group takes in drink B 100mL/ days；C group takes in drink A
100mL/ days.It is consecutively ingested 30 days, detects weight, significantly reduce 6.3% before C group weight is not drunk as the result is shown, and A
With the then all raisings of B group weight.
Drink A provided in this embodiment is the lower blood-fat and reduce weight drink with function based on indigestible carbohydrates, natural
It is nontoxic, there are multiple functions, for example maintain intestinal flora in a healthy and balanced way etc..
Embodiment 7
(1) preparation of indigestion carbohydrate enzymatic hydrolysis object:
Accurately weigh 0.2g mannase to be dissolved in 100mL distilled water, weigh 20g konjac polysaccharide, be slowly added in
In water, 40 DEG C of water-baths simultaneously stir enzymatic hydrolysis, until konjac polysaccharide is all dissolved into aqueous solution, obtain konjac polysaccharide hydrolysate A；
It accurately weighs 0.2g cellulase to be dissolved in 100mL distilled water, weighs 20g konjac polysaccharide, be slowly added in water
In, 40 DEG C of water-baths simultaneously stir enzymatic hydrolysis, until konjac polysaccharide is all dissolved into aqueous solution, obtain konjac polysaccharide hydrolysate B.
(2) preparation of the drink based on indigestible carbohydrates: taking konjac polysaccharide hydrolysate made from step (1),
It gradually adds stevioside and citric acid mixing is dissolved in the water, bottle, 121 DEG C of sterilizing at least 15min；Be subsequently cooled to 40 DEG C with
Under, obtain the drink based on indigestible carbohydrates, wherein the mass percent of each component in drink are as follows: konjac polysaccharide
Hydrolysate A or B 20%, stevioside 0.1%, citric acid 4%, water supplies 100%, respectively obtains drink A and drink B；
Control group is set simultaneously, the composition of control group is as follows: glucose 20%, stevioside 0.1%, citric acid 4%, and water is mended
Foot 100%.
(3) efficacy detection: it is divided into tri- groups of A, B, C using 30 25~30 years old male volunteers and carries out double blind reality as object
It tests, every group 10.Wherein, A group is taken in control group 100mL/ days, and B group takes in drink B 100mL/ days；C group takes in drink A
100mL/ days.It is consecutively ingested 30 days, detects weight, significantly reduce 5.6% before C group weight is not drunk as the result is shown, and B
With the then all raisings of C group weight.
Drink A provided in this embodiment is the lower blood-fat and reduce weight drink with function based on indigestible carbohydrates, natural
It is nontoxic, there are multiple functions, for example maintain intestinal flora in a healthy and balanced way etc..
Embodiment 8
(1) it the preparation of indigestion carbohydrate enzymatic hydrolysis object: accurately weighs 0.1g β -1,4- endoglucanase and is dissolved in
In 100mL distilled water, 5g Linseed pigment (flaxseed gum) being weighed, is slowly added in water, 40 DEG C of water-baths simultaneously stir enzymatic hydrolysis, until
Linseed pigment is all dissolved into aqueous solution, obtains flaxseed gum hydrolysate；
(2) ingredient preformulation: taking flaxseed gum hydrolysate made from step (1), gradually adds stevioside, citric acid and benzene
Sodium formate, potassium sorbate mixing are dissolved in the water, and obtain ingredient premix object
(3) preparation of jelly: compound gel (compounded and formed by xanthan gum and carragheen 9:1 ratio in mass ratio) is dissolved in
It in 90 DEG C of hot water, is mixed when being cooled to 50 DEG C or so with the ingredient premix object that step (2) obtains, and moisturizing is to quality percentage
Than being 100%.Wherein, the mass percent of each component are as follows: flaxseed gum hydrolysate 5%, stevioside 0.4%, citric acid
1.5%, compound gel 1%, sodium benzoate 0.05%, potassium sorbate 0.05%.
(4) efficacy detection: being divided into A using 20 25~30 years old male volunteers and two groups of B carry out double blind experiment as object,
Every group 10.Wherein, A group intake mass fraction is glucose solution 100mL/ days of 6.4%, and B group takes in this product 100g/
It.It is consecutively ingested 30 days, detects weight, significantly reduce 5.3% before B group weight is not drunk as the result is shown, and A group weight
Then all raisings.
Jelly B manufactured in the present embodiment is the natural nothing of lower blood-fat and reduce weight function jelly based on indigestible carbohydrates
Poison, with multiple functions, for example maintain intestinal flora in a healthy and balanced way etc..
Embodiment 9
(1) it the preparation of indigestion carbohydrate enzymatic hydrolysis object: accurately weighs 0.2g β -1,4- endoglucanase and is dissolved in
In 100mL distilled water, 10g Linseed pigment (flaxseed gum) being weighed, is slowly added in water, 40 DEG C of water-baths simultaneously stir enzymatic hydrolysis,
Aqueous solution is all dissolved into Linseed pigment；
(2) ingredient preformulation: taking flaxseed gum hydrolysate made from step (1), gradually adds stevioside, citric acid and benzene
Sodium formate, potassium sorbate mixing are dissolved in the water, and obtain ingredient premix object；
(3) preparation of jelly: compound gel (compounded and formed by xanthan gum and carragheen 9:1 ratio in mass ratio) is dissolved in
It in 80 DEG C of hot water, is mixed when being cooled to 50 DEG C or so with the ingredient premix object that step (2) obtains, and moisturizing is to quality percentage
Than being 100%.Wherein, the mass percent of each component are as follows: flaxseed gum hydrolysate 10%, stevioside 0.3%, citric acid
2.0%, compound gel 0.8%, sodium benzoate 0.05%, potassium sorbate 0.05%.
(4) efficacy detection: being divided into A using 20 25~30 years old male volunteers and two groups of B carry out double blind experiment as object,
Every group 10.Wherein, A group intake mass fraction is glucose solution 100mL/ days of 11.1%, and B group takes in this product
100g/ days.It is consecutively ingested 30 days, detects weight, significantly reduce 6.1% before B group weight is not drunk as the result is shown, and A
Group weight then all raisings.
Jelly B manufactured in the present embodiment is the natural nothing of lower blood-fat and reduce weight function jelly based on indigestible carbohydrates
Poison, with multiple functions, for example maintain intestinal flora in a healthy and balanced way etc..
Embodiment 10
(1) it the preparation of indigestion carbohydrate enzymatic hydrolysis object: accurately weighs 0.4g β -1,3- endoglucanase and is dissolved in
In 100mL distilled water, 20g laminarin is weighed, is slowly added in water, 40 DEG C of water-baths simultaneously stir enzymatic hydrolysis, until laminarin is complete
Portion is dissolved into aqueous solution；
(2) ingredient preformulation: taking laminarin hydrolysate made from step (1), gradually addition, stevioside, citric acid and
Sodium benzoate, potassium sorbate mixing are dissolved in the water, and obtain ingredient premix object；
(3) preparation of jelly: compound gel (compounded and formed by xanthan gum and carragheen 9:1 ratio in mass ratio) is dissolved in
It in 70 DEG C of hot water, is mixed when being cooled to 50 DEG C or so with the ingredient premix object that step (2) obtains, and moisturizing is to quality percentage
Than being 100%.Wherein, the mass percent of each component are as follows: laminarin hydrolysate 20%, stevioside 0.1%, citric acid
2.5%, compound gel 0.7%, sodium benzoate 0.05%, potassium sorbate 0.05%.
(4) efficacy detection: being divided into A using 20 25~30 years old male volunteers and tetra- groups of B carry out double blind experiment as object,
Every group 10.Wherein, A group intake mass fraction is glucose solution 100mL/ days of 20.8%, and B group takes in this drink
100g/ days.It is consecutively ingested 30 days, detects weight, significantly reduce 6.6% before B group weight is not drunk as the result is shown, and A
Group weight then all raisings.
Jelly B manufactured in the present embodiment is the natural nothing of lower blood-fat and reduce weight function jelly based on indigestible carbohydrates
Poison, with multiple functions, for example maintain intestinal flora in a healthy and balanced way etc..
Embodiment 11
(1) it the preparation of indigestion carbohydrate enzymatic hydrolysis object: accurately weighs 0.4g β -1,3- endoglucanase and is dissolved in
In 100mL distilled water, 30g laminarin is weighed, is slowly added in water, 40 DEG C of water-baths simultaneously stir enzymatic hydrolysis, until laminarin is complete
Portion is dissolved into aqueous solution；
(2) preparation of the lower blood-fat and reduce weight drink with function based on indigestible carbohydrates: thallus laminariae made from step (1) is taken
Polysaccharide hydrolysis object, gradually addition stevioside, citric acid and sodium benzoate, potassium sorbate mixing are dissolved in the water, and it is pre- to obtain ingredient
Mixture；
(3) preparation of jelly: by compound gel (compounded and formed by xanthan gum and carragheen 9:1 ratio in mass ratio) in boiling water
Middle dissolution mixes when being cooled to 50 DEG C or so with the ingredient premix object that step (2) obtains, and moisturizing to mass percent is
100%.The wherein mass percent of each component are as follows: laminarin hydrolysate 30%, stevioside 0.1%, citric acid 3%, compounding
Glue 0.6%, sodium benzoate 0.05%, potassium sorbate 0.05%.
(4) efficacy detection: being divided into A using 20 25~30 years old male volunteers and two groups of B carry out double blind experiment as object,
Every group 10.Wherein, A group intake mass fraction is glucose solution 100mL/ days of 30.7%, and B group takes in this product
100g/ days.It is consecutively ingested 30 days, detects weight, significantly reduce 10.7% before B group weight is not drunk as the result is shown, and A
Group weight then all raisings.
Jelly B manufactured in the present embodiment is the natural nothing of lower blood-fat and reduce weight drink with function based on indigestible carbohydrates
Poison, with multiple functions, for example maintain intestinal flora in a healthy and balanced way etc..
Embodiment 12
(1) it the preparation of indigestion carbohydrate enzymatic hydrolysis object: accurately weighs 0.5g mannase and is dissolved in 100mL distillation
In water, 30g konjac polysaccharide is weighed, is slowly added in water, 40 DEG C of water-baths simultaneously stir enzymatic hydrolysis, until konjac polysaccharide is all dissolved into water
Solution；
(2) preparation of the lower blood-fat and reduce weight drink with function based on indigestible carbohydrates: konjaku made from step (1) is taken
Polysaccharide hydrolysis object, gradually addition stevioside, citric acid and sodium benzoate, potassium sorbate mixing are dissolved in the water, and obtain ingredient
Premix object；
(3) ingredient preformulation: compound gel (compounded and formed by xanthan gum and carragheen 9:1 ratio in mass ratio) is dissolved in
It in 90 DEG C of hot water, is mixed when being cooled to 50 DEG C or so with the ingredient premix object that step (2) obtains, and moisturizing is to quality percentage
Than being 100%.Wherein, the mass percent of each component are as follows: konjac polysaccharide hydrolysate 40%, stevioside 0.1%, citric acid 4%,
Compound gel 0.4%, sodium benzoate 0.05%, potassium sorbate 0.05%.
(4) efficacy detection: it is divided into tetra- groups of A, B, C, D using 40 25~30 years old male volunteers and carries out double blind reality as object
It tests, every group 10.Wherein, A group intake mass fraction is glucose solution 100mL/ days of 30.5%, and B group takes in this product
100g/ days.It is consecutively ingested 30 days, detects weight, significantly reduce 10.5% before B group weight is not drunk as the result is shown, and A
Group weight then all raisings.
Jelly B manufactured in the present embodiment is the natural nothing of lower blood-fat and reduce weight drink with function based on indigestible carbohydrates
Poison, with multiple functions, for example maintain intestinal flora in a healthy and balanced way etc..
The above embodiment is a preferred embodiment of the present invention, but embodiments of the present invention are not by above-described embodiment
Limitation, other any changes, modifications, substitutions, combinations, simplifications made without departing from the spirit and principles of the present invention,
It should be equivalent substitute mode, be included within the scope of the present invention.
Claims (13)
1. a kind of preparation method of the water-disintegrable polysaccharide with lower blood-fat and reduce weight function, it is characterised in that include the following steps: to not
Digestible carbohydrates are digested, and enzymatic hydrolysis is all dissolved into aqueous solution to indigestible carbohydrates, obtain having drop
The water-disintegrable polysaccharide of rouge weight losing function.
2. the preparation method of the water-disintegrable polysaccharide according to claim 1 with lower blood-fat and reduce weight function, it is characterised in that:
The indigestible carbohydrates are at least one of flaxseed gum, konjak glucomannan and laminarin；
The enzyme is at least one of mannonase β -1,4- endoglucanase and β -1,3- endoglucanase.
3. the preparation method of the water-disintegrable polysaccharide according to claim 1 with lower blood-fat and reduce weight function, it is characterised in that:
The indigestible carbohydrates and the enzyme 40~120:1 in mass ratio proportion；
The temperature of the enzymatic hydrolysis is 35~45 DEG C.
4. the preparation method of the water-disintegrable polysaccharide according to claim 2 with lower blood-fat and reduce weight function, it is characterised in that:
When the indigestible carbohydrates are flaxseed gum, the enzyme is β-Isosorbide-5-Nitrae-endoglucanase；
When the indigestible carbohydrates are laminarin, the enzyme is β -1,3- endoglucanase；
When the indigestible carbohydrates are konjac polysaccharide, the enzyme is mannase.
5. the preparation method of the water-disintegrable polysaccharide according to claim 4 with lower blood-fat and reduce weight function, it is characterised in that:
The flaxseed gum and β -1,4- endoglucanase 40~60:1 in mass ratio proportion；
The laminarin and β -1,3- endoglucanase 60~90:1 in mass ratio proportion；
The konjac polysaccharide and the mannase 80~120:1 in mass ratio proportion.
6. a kind of water-disintegrable polysaccharide with lower blood-fat and reduce weight function, it is characterised in that: by described in any one of Claims 1 to 5
Preparation method obtain.
7. the water-disintegrable polysaccharide as claimed in claim 6 with lower blood-fat and reduce weight function has in lower blood-fat and reduce weight functional food in preparation
Application.
8. the water-disintegrable polysaccharide with lower blood-fat and reduce weight function according to claim 7 has lower blood-fat and reduce weight in preparation
Application in functional food, it is characterised in that: the food is solid beverage, meal replacement powder, drink, jelly or Yoghourt.
9. a kind of have effects that the drink of lower blood-fat and reduce weight, it is characterised in that: there is lower blood-fat and reduce weight function containing as claimed in claim 6
The water-disintegrable polysaccharide of energy.
10. a kind of have effects that the jelly of lower blood-fat and reduce weight, it is characterised in that: there is lower blood-fat and reduce weight containing as claimed in claim 6
The water-disintegrable polysaccharide of function.
11. a kind of have effects that the Yoghourt of lower blood-fat and reduce weight, it is characterised in that: there is lower blood-fat and reduce weight containing as claimed in claim 6
The water-disintegrable polysaccharide of function.
12. a kind of have effects that the solid beverage of lower blood-fat and reduce weight, it is characterised in that: there is lipid-loweringing containing as claimed in claim 6
The water-disintegrable polysaccharide of weight losing function.
13. a kind of have effects that the meal replacement powder of lower blood-fat and reduce weight, it is characterised in that: subtract containing as claimed in claim 6 with lipid-loweringing
The water-disintegrable polysaccharide of fertile function.
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Citations (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20110294166A1 (en) * | 2010-05-28 | 2011-12-01 | The Board Of Trustees Of The University Of Illinois | Thermostable enzymes for the hydrolysis of mannan-containing polysaccharides |
| CN105087717A (en) * | 2015-07-31 | 2015-11-25 | 丽江玉元食品有限公司 | Method of utilizing acidic mannase to hydrolyze refined konjac powder to prepare konjac oligosaccharide |
| CN106036642A (en) * | 2016-06-14 | 2016-10-26 | 王斐芬 | Method for preparing konjac flour |
| CN106689948A (en) * | 2016-11-30 | 2017-05-24 | 河北爱怡尚饮品有限公司 | Collagen composite linolenic acid beverage and preparation method thereof |
| CN106755012A (en) * | 2015-11-25 | 2017-05-31 | 中国科学院大连化学物理研究所 | A kind of endo beta-1,3-glucanase coding gene and its enzyme and preparation method and application |
| CN106943423A (en) * | 2017-03-23 | 2017-07-14 | 江苏省中医药研究院 | The polysaccharide composition acted on regulating intestinal canal Bacterial community and its application |
| CN107893061A (en) * | 2017-11-15 | 2018-04-10 | 中国农业大学 | A kind of dextranases of β 1,3 in rhizomucor miehei source and its application |
| CN109007712A (en) * | 2018-08-10 | 2018-12-18 | 广州钮和生物科技有限公司 | A kind of jelly and preparation method with lower blood-fat and reduce weight function |
| CN109156828A (en) * | 2018-08-10 | 2019-01-08 | 广州钮和生物科技有限公司 | A kind of drink and preparation method with lower blood-fat and reduce weight function |
-
2018
- 2018-08-10 CN CN201810905688.6A patent/CN109182415A/en active Pending
Patent Citations (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20110294166A1 (en) * | 2010-05-28 | 2011-12-01 | The Board Of Trustees Of The University Of Illinois | Thermostable enzymes for the hydrolysis of mannan-containing polysaccharides |
| CN105087717A (en) * | 2015-07-31 | 2015-11-25 | 丽江玉元食品有限公司 | Method of utilizing acidic mannase to hydrolyze refined konjac powder to prepare konjac oligosaccharide |
| CN106755012A (en) * | 2015-11-25 | 2017-05-31 | 中国科学院大连化学物理研究所 | A kind of endo beta-1,3-glucanase coding gene and its enzyme and preparation method and application |
| CN106036642A (en) * | 2016-06-14 | 2016-10-26 | 王斐芬 | Method for preparing konjac flour |
| CN106689948A (en) * | 2016-11-30 | 2017-05-24 | 河北爱怡尚饮品有限公司 | Collagen composite linolenic acid beverage and preparation method thereof |
| CN106943423A (en) * | 2017-03-23 | 2017-07-14 | 江苏省中医药研究院 | The polysaccharide composition acted on regulating intestinal canal Bacterial community and its application |
| CN107893061A (en) * | 2017-11-15 | 2018-04-10 | 中国农业大学 | A kind of dextranases of β 1,3 in rhizomucor miehei source and its application |
| CN109007712A (en) * | 2018-08-10 | 2018-12-18 | 广州钮和生物科技有限公司 | A kind of jelly and preparation method with lower blood-fat and reduce weight function |
| CN109156828A (en) * | 2018-08-10 | 2019-01-08 | 广州钮和生物科技有限公司 | A kind of drink and preparation method with lower blood-fat and reduce weight function |
Non-Patent Citations (6)
| Title |
|---|
| PRATIBHA KAUSHIK: "Effect of extraction temperature on composition, structure and functional properties of flaxseed gum", 《FOOD CHEMISTRY》 * |
| 国家药典委员会: "《中华人民共和国药典临床用药须知》", 31 October 2017 * |
| 朱俊玲: "功能性低聚糖的研究进展及其应用现状", 《食品研究与开发》 * |
| 李剑芳: "β-甘露聚糖酶制备魔芋葡甘露低聚糖的研究", 《食品与发酵工业》 * |
| 欧阳伶俐: "亚麻籽饼酶法脱胶工艺条件研究", 《中国油脂》 * |
| 潘道东: "《功能性食品添加剂》", 31 January 2006 * |
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