CN109169634A - The preservation liquid of human anatomy internal organ sample - Google Patents
The preservation liquid of human anatomy internal organ sample Download PDFInfo
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- CN109169634A CN109169634A CN201811181855.3A CN201811181855A CN109169634A CN 109169634 A CN109169634 A CN 109169634A CN 201811181855 A CN201811181855 A CN 201811181855A CN 109169634 A CN109169634 A CN 109169634A
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- Prior art keywords
- preservation liquid
- internal organ
- human anatomy
- organ sample
- preservation
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N1/00—Preservation of bodies of humans or animals, or parts thereof
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- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Dentistry (AREA)
- General Health & Medical Sciences (AREA)
- Wood Science & Technology (AREA)
- Zoology (AREA)
- Environmental Sciences (AREA)
- Agricultural Chemicals And Associated Chemicals (AREA)
Abstract
This case is related to a kind of preservation liquid of human anatomy internal organ sample, and saving liquid includes following component: diethylene glycol, polyethylene glycol 400, Osmolyte regulator, preservative, mould inhibitor, color preserving agent.Preservation liquid of the invention is free of formaldehyde, and volatilization is slow, has no irritating odor, and toxicity is very low, highly-safe, will not pollute experimental situation, preservation effect is good, and long period preservation will not go mouldy, and sample is soft, and color is good.
Description
Technical field
The present invention relates to a kind of anatomy field more particularly to a kind of preservation liquid of human anatomy internal organ sample.
Background technique
It is well known that making sample keep intact as far as possible by Saving specimen in transparency liquid, be conducive to scientific research, teaching
And people's is ornamental, used liquid is mainly formalin solution at present, and formalin solution is injected into the blood of human body
The corruption of corpse can be prevented in pipe.Formalin specimen preserving liquid has good fixed function, this is because formaldehyde and mark
The functional group of this protein largely combines, and long-term placement further polymerization forms paraformaldehyde.This process is allowed in albumen
Matter peptide interchain forms appropriate crosslinking, can sterilize and the tissue morphology of sample.But formaldehyde, benzene class are by generation
Boundary's health organization is determined as carcinogen, is listed in high poison chemicals, meeting intense stimulus eyes, skin, respiratory mucosa etc., most
It causes immunologic dysfunction, hepatic injury and central nervous system to be affected eventually, will lead to fetal anomaly, leukaemia, chronic exhales
The diseases such as tract disease, nasopharyngeal carcinoma, feminine menstrual disorder, acute confusion depression are inhaled, serious threat is caused to human health.
Simultaneously because formalin low boiling point, therefore the highly volatile in room temperature gives out strong as the rising evaporation rate of temperature is accelerated
Strong penetrating odor, seriously pollutes laboratory environment.Over time, air formaldehyde concentration also constantly increases, right
The health of medicine, biology and zoology worker, which causes, to be seriously threatened.In addition, the sample through formalin long-term preservation,
Phenomena such as mildew, xanthochromia, sample are hardened can occur.Formalin concentration can also reduce due to the volatilization of formaldehyde, the suppression to bacterium
Effect processed reduces, and gradually loses the function of saving sample.
Summary of the invention
For the technical problems in the prior art, this case provides a kind of preservation liquid of human anatomy internal organ sample,
The preservation liquid is free of formaldehyde, and volatilization is slow, has no irritating odor, and toxicity is very low, highly-safe, will not pollute experimental situation, protects
It is good to deposit effect, long period preservation will not go mouldy, and sample is soft, and color is good.
To achieve the above object, this case is achieved through the following technical solutions:
A kind of preservation liquid of human anatomy internal organ sample, wherein calculate by weight, the preservation liquid includes following group
Point:
Preferably, the preservation liquid of the human anatomy internal organ sample, wherein the preservative include 20~
24wt%5- amino indole, 36~40wt%4- (imidazoles -1- base) aniline, 25~30wt%4- mercaptoethyl pyridine, 10~
15wt% polyhexamethylene guanide.
Preferably, the preservation liquid of the human anatomy internal organ sample, wherein the mould inhibitor include 35~
40wt% zinc pyrithione, 16~20wt% potassium tartrate, 45~50wt% sodium iso-octoate.
Preferably, the preservation liquid of the human anatomy internal organ sample, wherein the Osmolyte regulator include 16~
22wt% dodecafluoroheptyl methacrylate, 24~28wt% myristic acid, 55~60wt% polyglycol ether.
Preferably, the preservation liquid of the human anatomy internal organ sample, wherein the color preserving agent is tea polyphenols.
Preferably, the preservation liquid of the human anatomy internal organ sample, wherein the molecule of the polyethylene glycol 400
Amount is 380~420.
Preferably, the preservation liquid of the human anatomy internal organ sample, wherein the preservation liquid further includes 0.05~
0.1 part of hydrocortisone.
Preferably, the preservation liquid of the human anatomy internal organ sample, wherein the preservation liquid further includes 0.5~1
Part disodium ethylene diamine tetraacetate.
The beneficial effects of the present invention are:
1) Novel specimen of the invention, which saves liquid, can effectively keep the tissue morphology and color of protozoa sample, non-volatile
Property substance, effect is lasting；
2) it saving liquid and is free of formaldehyde, volatilization is slow, has no irritating odor, and toxicity is very low, and it is highly-safe, experimental ring will not be polluted
Border, preservation effect is good, and long period preservation will not go mouldy, and sample is soft, and color is good, has reached goal of the invention.
Specific embodiment
The present invention will be further described in detail below with reference to the embodiments, to enable those skilled in the art referring to specification
Text can be implemented accordingly.
The present invention provides a kind of preservation liquid of human anatomy internal organ sample, calculates by weight, and the preservation liquid includes
Following component:
Zoological specimens body is rich in moisture and polar substances,
Diethylene glycol has stronger dehydration, can remove the moisture in cell surface portion, protein conformation is made to loosen,
And it solidifies protein denaturation and plays antisepsis；Polyethylene glycol 400 is the liquid of vicidity of no color or smell, energy and diethylene glycol
Any mixing, it is more stable to be formed by solution, therefore is the very high solvent of value and preservation liquid ingredient；Polyethylene glycol 400
Moisture retention is high, plays anti-drying effect, keeps tissue soft and transparent, and in saving liquid, polyethylene glycol 400 is a kind of good
Good selection, the hygroscopicity using polyethylene glycol 400 and the special solvent advantage to certain inorganic salts drugs, configuration
At preservation liquid stablize, human body specimen is not easy drying, and tissue is soft, reduce internal organ sample bring is hardened due to solidification it is secondary
Effect.More importantly polyethylene glycol 400 can adsorb mixed drug such as diethylene glycol, be trapped in sample inside and surface
Keep preservative not volatile and lost, enhance anti-corrosion effect, improves anti-corrosion ability and sterilizing ability.
Preferably, the preservative includes 20~24wt%5- amino indole, 36~40wt%4- (imidazoles -1- base) benzene
Amine, 25~30wt%4- mercaptoethyl pyridine, 10~15wt% polyhexamethylene guanide.5- amino indole passes through interference microorganism
Enzyme system, destroy its normal metabolism, inhibit the activity of enzyme, 4- mercaptoethyl pyridine makes the protein coagulating of microorganism
And denaturation, interfere it to survive and breed, polyhexamethylene guanide inhibits its intracorporal enzyme by changing cytoplasm permeability of the membrane
With the exclusion of metabolite, lead to its inactivation, three's collaboration reaches optimal anti-corrosion effect.
Preferably, the mould inhibitor include 35~40wt% zinc pyrithione, 16~20wt% potassium tartrate, 48~
50wt% sodium iso-octoate.Zinc pyrithione is a kind of chelate, it is effectively anti-mildew and antibacterial compound, pyridine sulphur
Ketone zinc, potassium tartrate and sodium iso-octoate collaboration have very strong inhibition spoilage organisms and mould effect, under acid and neutrallty condition all
Antisepsis can be given full play to, mould, the activity of saccharomycete and aerobic bacteria can be effectively inhibited, moreover it is possible to prevent meat poisoning bar
The growth of the harmful microbes such as bacterium, staphylococcus, salmonella and breeding, and have to anaerobic Bacillus and lactobacillus acidophilus etc.
Beneficial microorganism is nearly unavailable, and the effect for restraining development is more stronger than bactericidal effect, to reach the preservation for effectively extending and breaking even
Time.
Preferably, the Osmolyte regulator includes 16~22wt% dodecafluoroheptyl methacrylate, 24~28wt%
Myristic acid, 55~60wt% polyglycol ether.Dodecafluoroheptyl methacrylate, myristic acid, polyglycol ether collaboration exist
The stability for destroying cell membrane to a certain extent, improves its permeability, and do not change the shape of cell, is conducive to diethylene glycol pair
The fixed function of cell membrane improves the holding time of internal organ sample and its maintenance of appearance reset condition.
Preferably, the color preserving agent is tea polyphenols.Tea polyphenols are a kind of polycyclic aromatic hydrocarbons, can be divided into flavanol compound, hydroxyl
Base-[4]-flavanol compound, anthocyanin class, flavonoids, flavonols and phenolic acid class etc. has stronger antioxidation, can have
The bacterial spore polluted in effect killing haemodialysis control unit pipeline has efficient antibacterial and keeps biological to the preservation of biological sample
The effect in sample primary colors pool, conducive to the preparation to preserving fluid for biological sample；And zoological specimens body is rich in moisture and polar object
Matter, polyhydroxy base class color preserving agent can carry out effectively being chemically bonded therewith forms hydroxyl bond, so that reaching body protects Semu.
Preferably, the molecular weight of the polyethylene glycol 400 is 380~420.
Preferably, the preservation liquid further includes 0.05~0.1 part of hydrocortisone.Hydrocortisone surface and protein
Molecule is combined closely by nitrogen-atoms, the crosslinking of appropriateness can be carried out to human body specimen tissue protein, and then play fixed
Effect, keeps the original form of human body specimen and the pliability of original structure and toughness.And bacterial endotoxin is reduced to body
Damage improves body to the tolerance of toxin, effectively inhibits expansion when internal organ Saving specimen.
Preferably, the preservation liquid further includes 0.5~1 part of disodium ethylene diamine tetraacetate.Disodium ethylene diamine tetraacetate is again
It is called EDTA, is a kind of good compounding agent in chemistry, there are six coordination atom, the complexs of formation to be called chelating object second two for it
Amine tetraacethyl disodium can improve the stability of specimen preserving liquid.
1 specific embodiment of table and comparative example:
Preservation liquid and comparative example 1-5 prepared by 1-3 of the embodiment of the present invention are saved into liquid and are respectively implanted kidney Saving specimen one
After year, liquid, comparative example 1-5 are saved to Novel specimen prepared by inventive embodiments 1-3 from naked eyes and smell sense organ respectively
It saves liquid and sterile sample color, tissue suppleness, the mildew situation on preservation casing wall, expose the wet of the tissue for saving liquid
Situation and smell are spent, as a result such as table 2.
The comparison of each example of table 2 and comparative example to internal organ Saving specimen effect
Although the embodiments of the present invention have been disclosed as above, but its is not only in the description and the implementation listed
With it can be fully applied to various fields suitable for the present invention, for those skilled in the art, can be easily
Realize other modification, therefore without departing from the general concept defined in the claims and the equivalent scope, the present invention is simultaneously unlimited
In specific details.
Claims (8)
1. a kind of preservation liquid of human anatomy internal organ sample, which is characterized in that calculate by weight, under the preservation liquid includes
Column component:
2. the preservation liquid of human anatomy internal organ sample as described in claim 1, which is characterized in that the preservative includes 20
~24wt%5- amino indole, 36~40wt%4- (imidazoles -1- base) aniline, 25~30wt%4- mercaptoethyl pyridine, 10~
15wt% polyhexamethylene guanide.
3. the preservation liquid of human anatomy internal organ sample as described in claim 1, which is characterized in that the mould inhibitor includes 35
~40wt% zinc pyrithione, 16~20wt% potassium tartrate, 45~50wt% sodium iso-octoate.
4. the preservation liquid of human anatomy internal organ sample as described in claim 1, which is characterized in that the Osmolyte regulator packet
Include 16~22wt% dodecafluoroheptyl methacrylate, 24~28wt% myristic acid, 55~60wt% polyglycol ether.
5. the preservation liquid of human anatomy internal organ sample as described in claim 1, which is characterized in that the color preserving agent is that tea is more
Phenol.
6. the preservation liquid of human anatomy internal organ sample as described in claim 1, which is characterized in that the polyethylene glycol 400
Molecular weight be 380~420.
7. the preservation liquid of human anatomy internal organ sample as described in claim 1, which is characterized in that the preservation liquid further includes
0.05~0.1 part of hydrocortisone.
8. the preservation liquid of human anatomy internal organ sample as described in claim 1, which is characterized in that the preservation liquid further includes
0.5~1 part of disodium ethylene diamine tetraacetate.
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| Application Number | Priority Date | Filing Date | Title |
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| CN201811181855.3A CN109169634A (en) | 2018-10-11 | 2018-10-11 | The preservation liquid of human anatomy internal organ sample |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201811181855.3A CN109169634A (en) | 2018-10-11 | 2018-10-11 | The preservation liquid of human anatomy internal organ sample |
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| Publication Number | Publication Date |
|---|---|
| CN109169634A true CN109169634A (en) | 2019-01-11 |
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| CN201811181855.3A Pending CN109169634A (en) | 2018-10-11 | 2018-10-11 | The preservation liquid of human anatomy internal organ sample |
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| CN104904706A (en) * | 2015-04-22 | 2015-09-16 | 青岛农业大学 | Formaldehyde-free animal specimen fixed liquid and preparation method thereof |
| CN105010310A (en) * | 2015-07-20 | 2015-11-04 | 常州市好利莱光电科技有限公司 | Fadeless plant specimen preparation method |
| CN105052891A (en) * | 2015-07-16 | 2015-11-18 | 内蒙古科技大学包头医学院 | Long-stem storage method for anatomical visceral specimen |
| CN105340875A (en) * | 2015-12-16 | 2016-02-24 | 贵阳中医学院 | Specimen preserving fluid and preparation method thereof |
| CN105360108A (en) * | 2015-12-16 | 2016-03-02 | 贵阳中医学院 | Specimen preservation fluid good in preservation effect and preparation method of specimen preservation fluid |
| CN105532637A (en) * | 2016-01-07 | 2016-05-04 | 别永信 | Human body teaching specimen preserving fluid and preparing method thereof |
| CN108432740A (en) * | 2018-04-08 | 2018-08-24 | 黄名义 | A kind of preservation liquid of sample |
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2018
- 2018-10-11 CN CN201811181855.3A patent/CN109169634A/en active Pending
Patent Citations (11)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1233391A (en) * | 1998-04-30 | 1999-11-03 | 北京市燕化研新技术开发公司 | Preservation liquid for animal body specimen |
| CN101011050A (en) * | 2007-02-13 | 2007-08-08 | *** | Composition for corpse specimen conservation and its preparing process |
| CN102578073A (en) * | 2012-02-07 | 2012-07-18 | 王永红 | Preserving fluid for biological sample |
| CN104396940A (en) * | 2014-10-11 | 2015-03-11 | 张炳强 | Tissue sample preservative solution and preparation method thereof |
| CN104904706A (en) * | 2015-04-22 | 2015-09-16 | 青岛农业大学 | Formaldehyde-free animal specimen fixed liquid and preparation method thereof |
| CN105052891A (en) * | 2015-07-16 | 2015-11-18 | 内蒙古科技大学包头医学院 | Long-stem storage method for anatomical visceral specimen |
| CN105010310A (en) * | 2015-07-20 | 2015-11-04 | 常州市好利莱光电科技有限公司 | Fadeless plant specimen preparation method |
| CN105340875A (en) * | 2015-12-16 | 2016-02-24 | 贵阳中医学院 | Specimen preserving fluid and preparation method thereof |
| CN105360108A (en) * | 2015-12-16 | 2016-03-02 | 贵阳中医学院 | Specimen preservation fluid good in preservation effect and preparation method of specimen preservation fluid |
| CN105532637A (en) * | 2016-01-07 | 2016-05-04 | 别永信 | Human body teaching specimen preserving fluid and preparing method thereof |
| CN108432740A (en) * | 2018-04-08 | 2018-08-24 | 黄名义 | A kind of preservation liquid of sample |
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| PB01 | Publication | ||
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| RJ01 | Rejection of invention patent application after publication | ||
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Application publication date: 20190111 |