CN106359367A - Formaldehyde-free specimen tissue preserving fluid and preparation method thereof - Google Patents
Formaldehyde-free specimen tissue preserving fluid and preparation method thereof Download PDFInfo
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- CN106359367A CN106359367A CN201610758557.0A CN201610758557A CN106359367A CN 106359367 A CN106359367 A CN 106359367A CN 201610758557 A CN201610758557 A CN 201610758557A CN 106359367 A CN106359367 A CN 106359367A
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- Prior art keywords
- butanol
- glycerol
- methanol
- hexanol
- moschus
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N1/00—Preservation of bodies of humans or animals, or parts thereof
Abstract
The invention belongs to the technical field of specimen preserving fluids, and particularly relates to a formaldehyde-free specimen tissue preserving fluid for a medical human body tissue specimen example and a preparation method of the preserving fluid. The preserving fluid is prepared from, by mass, 20%-40% of methanol, 15%-36% of ethanol, 5%-23% of butanol, 3%-12% of hexanol, 7%-20% of glycerin, 1%-9% of potassium nitrate, 0.5%-6% of benzoic acid, 0.4%-5% of picric acid, 0.1%-2% of isothiazolone and 0.1%-3% of musk. The preserving fluid is poisonless, non-volatile and stable in nature, cannot generate precipitates and can effectively ensure high-quality preservation of the medical human body tissue dissection specimen example.
Description
Technical field
The invention belongs to specimen preserving liquid technical field, more particularly, to a kind of for tissue medical example specimen
No aldehyde specimen tissue preserration liquid and preparation method thereof.
Background technology
The formalin of 35%-40% concentration also known as formalin, because formalin is strong to tissue permeability, fixing equal
Even, in addition cheap, it is typically used to the fixing long-term preservation with specimen of embalming.But formalin there is also as follows
Shortcoming:
1), after the long-time placement of formalin, easily form paraformaldehyde and seem muddy and affect observation, therefore so that preserving liquid
In actual applications, need every half a year to change once new liquid；
2) formalin is larger to the damage of health, is mainly shown as to Mucocutaneous stimulation: formaldehyde energy and egg
White matter combines, high concentration the serious stimulation of respiratory tract and edema, Eye irritation, headache when sucking, so working as formaldehyde indoors
The sense of discomfort of people when reaching finite concentration, will be caused, and cause furious, ophthalmic pruritus, throat discomfort or pain, hoarseness,
The symptoms such as sneeze, uncomfortable in chest, asthma, dermatitis, and direct skin contact formaldehyde can cause allergic dermatitises, mottle, necrosis, sucks
Bronchial asthma can be induced during high-concentration formaldehyde.
3) formalin anti-mold effect is poor, and readily volatilized, and with the prolongation of use time, concentration reduces, and leads to
Antisepsises decline, and are unfavorable for the long-term preservation of specimen.
4) permeability that formalin is organized to specimen is strong, is easily caused specimen hardening and becomes fragile, for common corpse
Anti-corrosion impact less, but easily causes larger infringement to the tissue dissec of medical example.Medical example
Tissue dissec, in long-term preservation, needs to ensure the stereovision of its definition when observing and tissue, if its
Hardening becomes fragile or long period of soaking is in corrosive preservation liquid, and specimen will be caused with irreversible damage.And periodically more
The step changed, also easily causes physical damnification to specimen.
In prior art, how using organic acid or heavy metallic salt and compounding alcohols prepares impregnating fluid, reach replacement formal
The purpose of woods, but yet suffer from certain corrosivity and toxicity, and the cellularity of easy injury tissue, it is unfavorable for tissue
The specimen later stage is prepared into the section for observing.
Therefore, low toxicity, non-stimulated, non-environmental-pollution and efficient specimen tissue preserration liquid are updated and study, especially
It is the preservation liquid of the tissue dissec of medical example, be that contemporary medical science, biology techniques field personnel urgently solve
Problem certainly.
Content of the invention
It is an object of the invention to provide one kind no aldehyde specimen tissue preserration liquid and preparation method thereof, the present invention is nontoxic, no wave
Send out, and stable in properties, be not in precipitation, can effectively ensure that the high-quality of the tissue dissec of medical example is protected
Deposit.
For solving above-mentioned technical problem, the technical solution used in the present invention is as follows:
Design one kind no aldehyde specimen tissue preserration liquid, is made up of the raw material of following quality proportioning:
Methanol 20-40%, ethanol 15-36%, butanol 5-23%, hexanol 3-12%, glycerol 7-20%, potassium nitrate 1-9%, benzoic acid 0.5-
6%, picric acid 0.4-5%, isothiazolone 0.1-2%, Moschus 0.1-3%.
Preferably, described preservation liquid is made up of the raw material of following quality proportioning:
Methanol 28%, ethanol 35%, butanol 12%, hexanol 9%, glycerol 8%, potassium nitrate 5%, benzoic acid 2%, picric acid 0.5%, isothiazole
Ketone 0.3%, Moschus 0.2%.
Preferably, described preservation liquid is made up of the raw material of following quality proportioning:
Methanol 31%, ethanol 31%, butanol 14%, hexanol 8%, glycerol 9%, potassium nitrate 4.5%, benzoic acid 1.5%, picric acid 0.4%, different
Thiazolone 0.4%, Moschus 0.2%.
Preferably, described preservation liquid is made up of the raw material of following quality proportioning:
Methanol 36%, ethanol 30%, butanol 10%, hexanol 11%, glycerol 7%, potassium nitrate 3%, benzoic acid 2%, picric acid 0.6%, different thiophene
Oxazolone 0.2%, Moschus 0.2%.
Preferably, described preservation liquid is made up of the raw material of following quality proportioning:
Methanol 33%, ethanol 30%, butanol 15%, hexanol 10%, glycerol 8%, potassium nitrate 2%, benzoic acid 1%, picric acid 0.5%, different thiophene
Oxazolone 0.3%, Moschus 0.2%.
Preferably, described preservation liquid is made up of the raw material of following quality proportioning:
Methanol 32%, ethanol 31%, butanol 12%, hexanol 12%, glycerol 9%, potassium nitrate 2%, benzoic acid 1%, picric acid 0.5%, different thiophene
Oxazolone 0.4%, Moschus 0.1%.
Preferably, described preservation liquid is made up of the raw material of following quality proportioning:
Methanol 31%, ethanol 32%, butanol 13%, hexanol 10%, glycerol 10%, potassium nitrate 1%, benzoic acid 2%, picric acid 0.4%, different thiophene
Oxazolone 0.5%, Moschus 0.1%.
Preferably, described preservation liquid is made up of the raw material of following quality proportioning:
Methanol 29%, ethanol 33%, butanol 12%, hexanol 12%, glycerol 10%, potassium nitrate 1.5%, benzoic acid 1.5%, picric acid 0.6%,
Isothiazolone 0.3%, Moschus 0.1%.
Preferably, described preservation liquid is made up of the raw material of following quality proportioning:
Methanol 34%, ethanol 32%, butanol 10%, hexanol 10%, glycerol 9%, potassium nitrate 3%, benzoic acid 1%, picric acid 0.5%, different thiophene
Oxazolone 0.3%, Moschus 0.2%.
The preparation method of above-mentioned no aldehyde specimen tissue preserration liquid it is characterised in that taking each raw material by quality proportioning, and by nitre
Stirring in methanol put into by sour potassium, benzoic acid, picric acid, isothiazolone, Moschus, to be dissolved add afterwards completely ethanol, butanol, oneself
Alcohol, glycerol stir.
Constituent species in the present invention are more, and compounding effect is good, and function collocation is also very reasonable, have sterilization, anti-corrosion, ooze
Thoroughly, effect that softening, abnormal smells from the patient are adjusted, specific as follows:
Methanol is a kind of transparent, colourless, inflammable liquid, slightly alcohol smell, can be with water, ethanol, ether, benzene, acetone and mostly
Number organic solvent is miscible, and it serves as solvent, dehydration and antibacterial in this application, because it has stronger dehydrations, energy
The moisture of removing cell surface, not only can sterilize and be also prevented from specimen corruption.
Ethanol has stronger dehydrations, can remove the moisture in cell surface portion, so that protein conformation is loosened, and makes
Protein denaturation solidifies and plays antisepsises.
Butanol is a kind of liquid that is colourless, having fume taste, is slightly dissolved in water, with ethanol, ether and other multiple organic solvent
Miscible.As dehydrant.
Hexanol is colourless transparent liquid, has fruit aromatic fragrance.Can be miscible with ethanol and ether, it is slightly soluble in water, also may be used
It is dissolved in multiple organic solvents.Produce preservative as solvent and medical industry.
Glycerol is the liquid of no color or smell, arbitrarily can mix with water and ethanol, the solution that it is formed is more stable, glycerol
Hygroscopicity is high, plays anti-drying effect, makes tissue soft and transparent.The application utilizes the anti-corrosive properties of glycerol, hygroscopicity and right
The special solvent advantage of some inorganic salts medicines is so that specimen tissue keeps soft condition and is difficult drying, more important
Be glycerol can adsorb mixed medicine such as ethanol, isothiazolone etc. so as to be trapped in that specimen is internal and surface make anti-corrosion
Dosage form becomes protecting film, not only prevents drug effect volatilization and scatters and disappears, and can significantly increase antiseptic effect, improves sterilizing ability.
Potassium nitrate is colourless oblique square crystal or white crystalline powder, soluble in water, is dissolved in glycerol and liquefied ammonia, insoluble in second
Ether.Potassium nitrate is easily reduced into potassium nitrite by bacterial action, and plays color fixative and antibacterial effect.
Benzoic acid is scale or acicular crystal or the crystalline powder that white has mercerising, and benzoic acid serves as suppression in this application
Microbial inoculum and color preserving agent, efficiently can not only be suppressed to mycete, and can keep the effect in biological sample primary colors pool.
Picric acid is Light yellow crystals, odorlessness, bitter in the mouth.It is insoluble in carbon tetrachloride, is slightly soluble in Carbon bisulfide, is dissolved in heat
Water, ethanol, ether, are soluble in the organic solvents such as acetone, benzene, serve as softening agent in this application, can to the skin of specimen and
Tendon is softened, and prevents stiff and brittle.
Isothiazolone is a kind of broad-spectrum bactericidal effect preservative, can effectively kill algae, antibacterial and funguses.Mechanism of action be with
After microorganism contact, antibacterial being disconnected and the protein of algae is bonded, irreversibly suppressing the growth of microorganism, thus leading
Cause the death of microbial cell, to common bacteria (sulfate reducting bacteria, sludge molding bacterium), funguses (iron-oxidizing bacteria, mycete, ferment
Female bacterium), algae etc. there is very strong suppression and killing action.Additionally, isothiazolone also has safe operation, stability is strong, make
The features such as use low cost.
Moschus is that the secretions drying in the male scent gland capsule of animal in deer family Moschus moschiferouss forms, and is a kind of fine perfumery, Moschus virtue
Fragrant pleasant, lasting fragrance, serves as osmo-regulators and antibacterial in this application, can neutralize micro- abnormal smells from the patient that alcohols gives out,
Improve working environment.
The present invention compared with prior art, has the advantage that the present invention is not volatile and has no irritating odor, colourless clear
Clearly, and to specimen organize non-corrosiveness, substantially improve the environment that specimen is deposited；Enable to specimen water suction moisturizing, be difficult to do
Dry, keep soft condition, the tissue dissec of especially medical example, it can be made to be methodically arranged and present, can be multiple
Reuse, should not damage.
Brief description
Fig. 1 is to preserve, using described in embodiment 1, the endometrial tissue that liquid preserves, after carrying out histopathologic slide's dyeing
Microphotograph；
Fig. 2 is to preserve, using described in embodiment 1, the cervical cancer tissues that liquid preserves, and carries out the microscope after histopathologic slide's dyeing
Photo；
Fig. 3 is to preserve, using described in embodiment 1, the breast cancer tissue that liquid preserves, and carries out the microscope after histopathologic slide's dyeing
Photo；
Fig. 4 is to preserve, using described in embodiment 1, the cervical tissue that liquid preserves, and carries out the microscope after histopathologic slide's dyeing and shines
Piece；
Fig. 5 is to preserve, using described in embodiment 1, the prostata tissue that liquid preserves, and carries out the microscope after histopathologic slide's dyeing
Photo；
Fig. 6 is using the cervical squamous intraepithelial tissue preserving liquid preservation described in embodiment 1, after carrying out histopathologic slide's dyeing
Microphotograph.
Specific embodiment
In order that the objects, technical solutions and advantages of the present invention become more apparent, below in conjunction with specific embodiment, to this
Invention is further elaborated.It should be appreciated that specific embodiment described herein is only in order to explain the present invention, not
For limiting the present invention.
Embodiment 1
No aldehyde specimen tissue preserration liquid, is made up of the raw material of following quality proportioning:
Methanol 28%, ethanol 35%, butanol 12%, hexanol 9%, glycerol 8%, potassium nitrate 5%, benzoic acid 2%, picric acid 0.5%, isothiazole
Ketone 0.3%, Moschus 0.2%.
Take each raw material by quality proportioning, and potassium nitrate, benzoic acid, picric acid, isothiazolone, Moschus are put in methanol and stirred
Mix, add ethanol, butanol, hexanol, glycerol to stir after being completely dissolved completely.
Embodiment 2
No aldehyde specimen tissue preserration liquid, is made up of the raw material of following quality proportioning:
Methanol 31%, ethanol 31%, butanol 14%, hexanol 8%, glycerol 9%, potassium nitrate 4.5%, benzoic acid 1.5%, picric acid 0.4%, different
Thiazolone 0.4%, Moschus 0.2%.
Take each raw material by quality proportioning, and potassium nitrate, benzoic acid, picric acid, isothiazolone, Moschus are put in methanol and stirred
Mix, add ethanol, butanol, hexanol, glycerol to stir after being completely dissolved completely.
Embodiment 3
No aldehyde specimen tissue preserration liquid, is made up of the raw material of following quality proportioning:
Methanol 36%, ethanol 30%, butanol 10%, hexanol 11%, glycerol 7%, potassium nitrate 3%, benzoic acid 2%, picric acid 0.6%, different thiophene
Oxazolone 0.2%, Moschus 0.2%.
Take each raw material by quality proportioning, and potassium nitrate, benzoic acid, picric acid, isothiazolone, Moschus are put in methanol and stirred
Mix, add ethanol, butanol, hexanol, glycerol to stir after being completely dissolved completely.
Embodiment 4
No aldehyde specimen tissue preserration liquid, is made up of the raw material of following quality proportioning:
Methanol 33%, ethanol 30%, butanol 15%, hexanol 10%, glycerol 8%, potassium nitrate 2%, benzoic acid 1%, picric acid 0.5%, different thiophene
Oxazolone 0.3%, Moschus 0.2%.
Take each raw material by quality proportioning, and potassium nitrate, benzoic acid, picric acid, isothiazolone, Moschus are put in methanol and stirred
Mix, add ethanol, butanol, hexanol, glycerol to stir after being completely dissolved completely.
Embodiment 5
No aldehyde specimen tissue preserration liquid, is made up of the raw material of following quality proportioning:
Methanol 32%, ethanol 31%, butanol 12%, hexanol 12%, glycerol 9%, potassium nitrate 2%, benzoic acid 1%, picric acid 0.5%, different thiophene
Oxazolone 0.4%, Moschus 0.1%.
Take each raw material by quality proportioning, and potassium nitrate, benzoic acid, picric acid, isothiazolone, Moschus are put in methanol and stirred
Mix, add ethanol, butanol, hexanol, glycerol to stir after being completely dissolved completely.
Embodiment 6
No aldehyde specimen tissue preserration liquid, is made up of the raw material of following quality proportioning:
Methanol 31%, ethanol 32%, butanol 13%, hexanol 10%, glycerol 10%, potassium nitrate 1%, benzoic acid 2%, picric acid 0.4%, different thiophene
Oxazolone 0.5%, Moschus 0.1%.
Take each raw material by quality proportioning, and potassium nitrate, benzoic acid, picric acid, isothiazolone, Moschus are put in methanol and stirred
Mix, add ethanol, butanol, hexanol, glycerol to stir after being completely dissolved completely.
Embodiment 7
No aldehyde specimen tissue preserration liquid, is made up of the raw material of following quality proportioning:
Methanol 29%, ethanol 33%, butanol 12%, hexanol 12%, glycerol 10%, potassium nitrate 1.5%, benzoic acid 1.5%, picric acid 0.6%,
Isothiazolone 0.3%, Moschus 0.1%.
Take each raw material by quality proportioning, and potassium nitrate, benzoic acid, picric acid, isothiazolone, Moschus are put in methanol and stirred
Mix, add ethanol, butanol, hexanol, glycerol to stir after being completely dissolved completely.
Embodiment 8
No aldehyde specimen tissue preserration liquid, is made up of the raw material of following quality proportioning:
Methanol 34%, ethanol 32%, butanol 10%, hexanol 10%, glycerol 9%, potassium nitrate 3%, benzoic acid 1%, picric acid 0.5%, different thiophene
Oxazolone 0.3%, Moschus 0.2%.
Take each raw material by quality proportioning, and potassium nitrate, benzoic acid, picric acid, isothiazolone, Moschus are put in methanol and stirred
Mix, add ethanol, butanol, hexanol, glycerol to stir after being completely dissolved completely.
Effect example
The no aldehyde specimen tissue preserration liquid that prepare the embodiment of the present invention 1 and 10% formalin carry out Saving specimen effect comparison.
1) it is respectively put into the heart, lung, kidney, spleen, brain, colon, the upper arm two in no aldehyde specimen tissue preserration liquid and formalin
Flesh specimen, preserves 1 year.Soft to the color of specimen, abnormal smells from the patient, the mildew preserving on casing wall, tissue from naked eyes and olfactory organoleptic
Degree carries out judgement and is summarized as follows table:
Table 1 no aldehyde tissue fixative solution and 10% formaldehyde preserve sample effectiveness comparison
2) skeletal muscle, intestinal, the heart, lung, liver, kidney and cerebral tissue are left in no aldehyde specimen group prepared by the embodiment of the present invention 1 respectively
Knit preservation liquid and 10% formalin in, 20 DEG C of room temperature, deposit 30 days, observe antiseptic effect, and experiment the 3rd day, 7 days, 14
My god, 30 days when, cut skeletal muscle, intestinal, the heart, lung, liver, kidney and cerebral tissue respectively and carry out pathological section preparation, and seen with microscope
Examine.
Tissue morphology pathological section microscopy images are as follows:
The pneumonocyte nuclear volume that no aldehyde specimen tissue preserration liquid preserves is 21.2 ± 2.6, the liver cell nuclear quantity of preservation is 121.4
±16.2；The pneumonocyte nuclear volume that 10% formalin preserves is 5.8 ± 4.3, the liver cell nuclear quantity of preservation is 62.3 ± 5.6；
No aldehyde specimen tissue preserration liquid preserves lung liver cell nuclear quantity and is significantly more than 10% formalin.
The specimen tissue morphology pathological change that no aldehyde specimen tissue preserration liquid preserves is visible under the microscope, cerebral tissue structure
Substantially normal, the core of neurocyte is clear or small part core disappears, and interstitial structure understands；Intestinal tissue each layer great majority are normal, carefully
Karyon great majority are clear；Glomerule inner cell core is clear, minority disappears；Renal cellses core is clear, minority disappears, interstitial
Structure understands；Skeletal Muscle Cell core is most of normal or all disappears, and myocyte's band is regional to disappear, and interstitial major part is just
Often.
For evaluating the present invention no killing effect to microorganism for the aldehyde specimen tissue preserration liquid, according to disinfection technology standard
(version in 2002), has carried out Guantitative bactericidal test.Test strain selects staphylococcus aureuses (staphylococcus
Aureus) atcc 6538, escherichia coli (escherichia coli) atcc 8099, Candida albicans (candida
albicans)atcc 10231 .By the concentration specified and action time, repeat to test 3 times, suspension quantitatively kills test
(see Table 2), equal to the staphylococcus aureuses and escherichia coli killing logarithm value of each time >=5.00, to each examination of Candida albicans
All >=4.00, it is qualified to be judged to sterilize for the killing logarithm value tested.
Table 2(note: "/" represent do not detect)
Claims (10)
1. no aldehyde specimen tissue preserration liquid is it is characterised in that be made up of the raw material of following quality proportioning:
Methanol 20-40%, ethanol 15-36%, butanol 5-23%, hexanol 3-12%, glycerol 7-20%, potassium nitrate 1-9%, benzoic acid 0.5-
6%, picric acid 0.4-5%, isothiazolone 0.1-2%, Moschus 0.1-3%.
2. preservation liquid as claimed in claim 1 is it is characterised in that be made up of the raw material of following quality proportioning:
Methanol 28%, ethanol 35%, butanol 12%, hexanol 9%, glycerol 8%, potassium nitrate 5%, benzoic acid 2%, picric acid 0.5%, isothiazole
Ketone 0.3%, Moschus 0.2%.
3. preservation liquid as claimed in claim 1 is it is characterised in that be made up of the raw material of following quality proportioning:
Methanol 31%, ethanol 31%, butanol 14%, hexanol 8%, glycerol 9%, potassium nitrate 4.5%, benzoic acid 1.5%, picric acid 0.4%, different
Thiazolone 0.4%, Moschus 0.2%.
4. preservation liquid as claimed in claim 1 is it is characterised in that be made up of the raw material of following quality proportioning: methanol 36%, second
Alcohol 30%, butanol 10%, hexanol 11%, glycerol 7%, potassium nitrate 3%, benzoic acid 2%, picric acid 0.6%, isothiazolone 0.2%, Moschus
0.2%.
5. preservation liquid as claimed in claim 1 is it is characterised in that be made up of the raw material of following quality proportioning: methanol 33%, second
Alcohol 30%, butanol 15%, hexanol 10%, glycerol 8%, potassium nitrate 2%, benzoic acid 1%, picric acid 0.5%, isothiazolone 0.3%, Moschus
0.2%.
6. preservation liquid as claimed in claim 1 is it is characterised in that be made up of the raw material of following quality proportioning: methanol 32%, second
Alcohol 31%, butanol 12%, hexanol 12%, glycerol 9%, potassium nitrate 2%, benzoic acid 1%, picric acid 0.5%, isothiazolone 0.4%, Moschus
0.1%.
7. preservation liquid as claimed in claim 1 is it is characterised in that be made up of the raw material of following quality proportioning: methanol 31%, second
Alcohol 32%, butanol 13%, hexanol 10%, glycerol 10%, potassium nitrate 1%, benzoic acid 2%, picric acid 0.4%, isothiazolone 0.5%, Moschus
0.1%.
8. preservation liquid as claimed in claim 1 is it is characterised in that be made up of the raw material of following quality proportioning: methanol 29%, second
Alcohol 33%, butanol 12%, hexanol 12%, glycerol 10%, potassium nitrate 1.5%, benzoic acid 1.5%, picric acid 0.6%, isothiazolone 0.3%,
Moschus 0.1%.
9. preservation liquid as claimed in claim 1 is it is characterised in that be made up of the raw material of following quality proportioning: methanol 34%, second
Alcohol 32%, butanol 10%, hexanol 10%, glycerol 9%, potassium nitrate 3%, benzoic acid 1%, picric acid 0.5%, isothiazolone 0.3%, Moschus
0.2%.
10. the preparation method of no aldehyde specimen tissue preserration liquid described in claim 1-9 is it is characterised in that taken respectively by quality proportioning
Raw material, and potassium nitrate, benzoic acid, picric acid, isothiazolone, Moschus are put into stirring in methanol, to be dissolved adds second completely afterwards
Alcohol, butanol, hexanol, glycerol stir.
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| CN201610758557.0A CN106359367A (en) | 2016-08-30 | 2016-08-30 | Formaldehyde-free specimen tissue preserving fluid and preparation method thereof |
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| CN107197857A (en) * | 2017-06-21 | 2017-09-26 | 上海于泽生物科技有限公司 | A kind of efficient specimen anti-corrosion preserves liquid and preparation method thereof |
| CN109452261A (en) * | 2018-12-27 | 2019-03-12 | 福建医科大学 | A kind of specimen preserving liquid and preparation method thereof |
| CN110987573A (en) * | 2019-12-23 | 2020-04-10 | 苏州堪赛尔医学检验有限公司 | Brain tissue fixing liquid and preparation method and application method thereof |
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| CN101496511A (en) * | 2008-01-31 | 2009-08-05 | 任祖翰 | Antisepsis composition for remains as well as preparation method and application |
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| CN107197857A (en) * | 2017-06-21 | 2017-09-26 | 上海于泽生物科技有限公司 | A kind of efficient specimen anti-corrosion preserves liquid and preparation method thereof |
| CN109452261A (en) * | 2018-12-27 | 2019-03-12 | 福建医科大学 | A kind of specimen preserving liquid and preparation method thereof |
| CN110987573A (en) * | 2019-12-23 | 2020-04-10 | 苏州堪赛尔医学检验有限公司 | Brain tissue fixing liquid and preparation method and application method thereof |
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