CN109160849B - Preparation method of solid microbial inoculum for pine trees - Google Patents
Preparation method of solid microbial inoculum for pine trees Download PDFInfo
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- CN109160849B CN109160849B CN201811169764.8A CN201811169764A CN109160849B CN 109160849 B CN109160849 B CN 109160849B CN 201811169764 A CN201811169764 A CN 201811169764A CN 109160849 B CN109160849 B CN 109160849B
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05G—MIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
- C05G3/00—Mixtures of one or more fertilisers with additives not having a specially fertilising activity
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05B—PHOSPHATIC FERTILISERS
- C05B7/00—Fertilisers based essentially on alkali or ammonium orthophosphates
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05G—MIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
- C05G5/00—Fertilisers characterised by their form
- C05G5/10—Solid or semi-solid fertilisers, e.g. powders
- C05G5/12—Granules or flakes
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- Organic Chemistry (AREA)
- Life Sciences & Earth Sciences (AREA)
- Pest Control & Pesticides (AREA)
- Fertilizers (AREA)
- Agricultural Chemicals And Associated Chemicals (AREA)
Abstract
The invention relates to the field of biological bacterial manure microbial inoculum, in particular to a preparation method of a solid microbial inoculum for pine trees. The preparation method comprises the following preparation steps: 1) melting and mixing polylactic acid and a fine-powder fertilizer to obtain a polylactic acid/fertilizer composite material, and then foaming to obtain a polylactic acid/fertilizer open-cell composite foaming material; 2) grafting sodium methacrylate on the surface of the foaming material; 3) and mixing and culturing the yellow aschersonia solution and the foaming material to obtain the yellow aschersonia solid microbial inoculum. According to the invention, the polylactic acid/fertilizer open-cell composite foaming material is used as a carrier, fertilizer fine powder is coated in the wall of the hole in the foaming material, a large amount of yellow aschersonia is adsorbed on the surface of the wall of the hole, the yellow aschersonia on the surface of the wall of the hole is released firstly when the porous composite foaming material is used, then the fertilizer fine powder is controlled to be released along with the degradation of the polylactic acid/fertilizer open-cell composite foaming material, and the used materials are biodegradable materials, so that the porous composite foaming material cannot remain in soil.
Description
Technical Field
The invention relates to the field of biological bacterial manure microbial inoculum, in particular to a preparation method of a solid microbial inoculum for pine trees.
Background
Mycorrhiza is a union formed by mycorrhizal fungi hypha in natural soil and higher plant nutrition root system, and is the most common symbiosis phenomenon of compatibility of soil microorganisms and plants. The symbiotic fungi obtain necessary carbohydrate and other nutrients from the plant body, and the plant also obtains the required nutrients, water, etc. from the fungi, so as to reach a highly unified union with mutual benefits and communication, which has the characteristics of both common plant root systems and obligate fungi. The rhizobacteria are divided into inner mycorrhiza, outer mycorrhiza and inner and outer mycorrhiza, the outer mycorrhiza has great promotion effect on the growth of forest trees, most outer mycorrhiza are connected with an underground hypha network system through epitaxial hypha, the hypha network is a main absorption organ of the mycorrhiza, and under the condition of good forest land, a large and complex hypha network system is arranged under the general underground. When the fungal mycelium distributed in the soil is infected, under the enzymatic action of hydrolases such as cellulase, pectinase, amylase, fatty acid and other carbohydrases secreted by the fungal mycelium, the fungal mycelium enters into the cell gap between cortex from the root tip and the root hair region of the forest tree root through the infection point to grow and propagate, the fungal mycelium located between the outer cortex and the cell gap between the outer cortex is Harti's net, and meanwhile, the secondary mycelium distributed on the outer surface of the root outer cortex is also propagated to form a fungal sleeve to wrap the root tip, so that the infection of the fungi on the root is completed, and the forest mycorrhiza is formed. Once a tree has formed a mycorrhiza with an ectomycorrhizal fungus, it is in communication with the network system. Research shows that the investment benefit of the trees for putting photosynthetic products into the rhizopus hyphae is much higher than the investment benefit of putting the photosynthetic products into the roots. With the same input, the hypha absorption area and length were 10 and 1000 times greater than those of the root system. Besides the required biological bacterial fertilizer, the chemical fertilizer is also a necessary fertilizer for forest growth, and in the agricultural production process, the biological bacterial fertilizer and the chemical fertilizer can not meet the requirements of crops in different growth stages due to one-time fertilization, so that multiple times of fertilization are often needed, the fertilizer utilization rate of the chemical fertilizer is low, and the environment is also polluted. In the prior art, the technical means that biological bacterial manure and chemical fertilizer are simultaneously loaded in a carrier and are not mutually influenced is difficult to find, so that the research of a carrier for scientific fertilization increases the utilization rate of the fertilizer, reduces the using amount and the fertilization times of the fertilizer, saves labor force, reduces environmental pollution and improves the quality of agricultural products, which is an important trend of fertilizer development.
The Chinese patent office discloses a pine ectomycorrhizal fungi capsule microbial inoculum and an invention patent of application thereof in 6 months and 29 days in 2012, and the granted publication number is CN101889587B, and the invention discloses the pine ectomycorrhizal fungi capsule microbial inoculum which is prepared by the following preparation process: pulverizing pine ectomycorrhizal fungi mycelia in a homogenizer, and fully and uniformly mixing the pulverized pine ectomycorrhizal fungi mycelia with a mixture of a sodium alginate solution and activated carbon according to the volume ratio of 2-3: 5 to form a mycorrhizal fungi glue solution; then adding the mycorrhizal fungi glue solution dropwiseCaCl2Forming spherical colloidal particles in the solution, solidifying for 24-30 h, and washing with sterile water to obtain the product. The invention also discloses application of the pine ectomycorrhizal fungi capsule microbial inoculum in promoting pine growth. The mycorrhizal fungi capsule agent has high activity, simple preparation process, convenient material source, easy preservation and easy industrial production, has good application prospect, ensures that the storage life of hypha is prolonged in the storage process, but only prepares biological bacterial manure into capsules, when in agricultural production and fertilization, in order to meet the requirements of pine trees on biological bacterial manure and chemical fertilizer, the fertilizer needs to be respectively fertilized for many times, the fertilization times are increased, the consumption of manpower and material resources in the agricultural production is also increased, moreover, the fertilizer after being fertilized is seriously wasted and has low utilization rate, the fertilization cost is increased, and the environmental and human health are threatened, therefore, the research of a carrier for scientific fertilization can increase the utilization rate of the fertilizer, reduce the using amount and the fertilization times of the fertilizer, save labor force, reduce environmental pollution and improve the quality of agricultural products, which is an important trend of fertilizer development.
Disclosure of Invention
In order to solve the problems that in the prior art, the fertilizer usage amount is high, the fertilization times are multiple, the fertilizer and manpower and material resources are seriously wasted, the requirement of crops on different fertilizers in different growth periods cannot be met, the requirement of simultaneously loading biological bacterial manure and chemical fertilizer cannot be met, and mutual influence is avoided, the solid microbial inoculum with the inner layer arranged in the degradable foaming material hole and the outer layer adsorbed with the biological bacterial manure is provided.
In order to achieve the purpose, the invention adopts the following technical scheme:
a preparation method of a solid microbial inoculum for pine trees comprises the following preparation steps:
(1) drying the fertilizer and grinding into fine powder;
(2) melting and mixing polylactic acid and the fine powder fertilizer at the temperature of 190-220 ℃ for 0.5-1.5h to obtain a polylactic acid/fertilizer composite material;
(3) foaming the polylactic acid/fertilizer composite material to obtain a polylactic acid/fertilizer open-cell composite foam material;
(4) carrying out plasma treatment on the polylactic acid/fertilizer open-cell composite foaming material;
(5) grafting sodium methacrylate on the surface of the polylactic acid/fertilizer open-cell composite foam material;
(6) placing the yellow aphrodisiac strain into a culture medium for standing culture to obtain a yellow aphrodisiac strain liquid;
(7) and mixing and culturing the yellow aschersonia solution and the sterilized polylactic acid/fertilizer open-cell composite foaming material to obtain the yellow aschersonia solid microbial inoculum.
The solid microbial inoculum of the invention takes a polylactic acid foaming material as a carrier, fertilizer fine powder is coated in a pore wall in the polylactic acid foaming material, a large amount of yellow aschersonia is adsorbed on the surface of the pore wall, when the inventor conducts invention creation, the fertilizer fine powder is inevitably exposed on the surface of the pore wall, when the polylactic acid foaming material is placed in the yellow aschersonia solution for culture and adsorption, the fertilizer fine powder can reduce the survival rate of the yellow aschersonia in the solution, finally the effective bacteria amount in the polylactic acid foaming material is reduced, and the adsorption amount of the yellow aschersonia is lower, therefore, the inventor grafts sodium methacrylate on the surface of the polylactic acid foaming material before the polylactic acid foaming material is placed in the yellow aschersonia solution for culture, at the moment, a layer of sodium polymethacrylate film is grown on the surface of the polylactic acid foaming material, the sodium polymethacrylate film can isolate the fertilizer fine powder and the yellow ascher, the fertilizer fine powder does not influence yellow aschersonia, and the sodium polymethacrylate film can also promote the polylactic acid foaming material to adsorb the yellow aschersonia, so that the solid microbial inoculum prepared by the method has better effect. When the solid microbial inoculum is applied to pine tree planting, the solid microbial inoculum is added into soil, yellow aschersonia adsorbed on the surface of the hole wall is connected with an underground hypha network system, then the polylactic acid foaming material is gradually degraded to release the fertilizer, the slow degradation process of the polylactic acid foaming material for releasing the fertilizer also plays a role in controlling the release of the fertilizer, the fertilizer period is prolonged, the absorption utilization rate of the fertilizer is improved, and the polylactic acid foaming material cannot remain in the soil after being completely degraded, so that the solid microbial inoculum is green and environment-friendly.
Preferably, the fertilizer in the step (1) comprises 10-15 parts of urea, 30-40 parts of diammonium phosphate, 30-40 parts of potassium chloride and 10-15 parts of zinc sulfate by weight.
Various fertilizers are reasonably matched, the controlled release of the fertilizers can be realized through the degradation of the polylactic acid foaming material, and the requirements of the pine trees on nitrogen fertilizers, potassium fertilizers, phosphate fertilizers and trace elements at different stages are met.
Preferably, the foaming of the polylactic acid/fertilizer composite material in the step (3) comprises the following steps: a) placing the polylactic acid/fertilizer composite material in a reaction kettle, and introducing carbon dioxide or nitrogen to the pressure of 8-15 Mpa; b) heating the reaction kettle to 80-120 ℃, and standing for 6-12 h; c) and (3) releasing the pressure of the reaction kettle, and cooling in ice bath for 1-5min to obtain the polylactic acid/fertilizer open-cell composite foam material.
Melting, blending and granulating polylactic acid and fine fertilizer to obtain the polylactic acid/fine fertilizer composite material, placing the composite material in a reaction kettle, introducing carbon dioxide or nitrogen to the pressure of 8-15Mpa, heating the reaction kettle to 80-120 ℃, standing for 6-12h to enable the composite material to fully absorb the carbon dioxide or the nitrogen, then quickly relieving the pressure of the reaction kettle, enabling the carbon dioxide or the nitrogen in the polylactic acid/fine fertilizer composite material to escape out of the polylactic acid/fine fertilizer composite material due to sudden pressure drop to form foam holes, cooling in ice bath for 1-5min, and sizing the foam holes to obtain the polylactic acid/fertilizer open-cell composite foamed material.
Preferably, the pressure relief rate is 25-40 MPa/s.
The pressure relief rate can control the diameter of the foam pores, and the adsorption of aschersonia chromocor is better in the pressure relief rate of 25-40 MPa/s.
Preferably, the sodium methacrylate grafting comprises the following steps: a) immersing the polylactic acid/fertilizer open-cell composite foaming material subjected to plasma treatment in water, adding 3-aminopropyltriethoxysilane under the protection of argon, reacting for 12-24h, taking out the foaming material, and drying; b) immersing the dried foam material in dichloromethane and triethylamine, dropwise adding 2-bromoisobutyryl bromide under the protection of argon gas, reacting for 12-24h, taking out the foam material, sequentially rinsing with water and acetone, and drying; c) adding sodium methacrylate and bipyridyl into deionized water, standing for 5-10min until the sodium methacrylate and bipyridyl are completely dissolved, then adding cupric bromide and cuprous bromide, completely immersing the foamed material in the system, reacting for 10-15h under the protection of argon, then taking out the foamed material, and sequentially cleaning with water and methanol to obtain the sodium methacrylate grafted polylactic acid/fertilizer open-cell composite foamed material.
And carrying out plasma treatment on the obtained polylactic acid/fertilizer open-cell composite foaming material to ensure that the surface of the polylactic acid/fertilizer open-cell composite foaming material is rich in hydroxyl, then adding 3-aminopropyltriethoxysilane under the protection of argon, reacting for 12-24h to obtain an aminated functional surface, then adding an initiator bromoisobutyryl bromide, reacting with amino at 0-2 ℃ to graft the initiator on the surface of the polylactic acid/fertilizer open-cell composite foaming material to obtain a brominated foaming material, then adding sodium methacrylate and bipyridine, adding cuprous bromide and copper bromide, reacting at normal temperature for 10-15h under the protection of vacuum, and sequentially washing with water and methanol to obtain the sodium polymethacrylate grafted polylactic acid/fertilizer open-cell composite foaming material.
Preferably, the graft thickness of the sodium polymethacrylate is 40-75 nm.
When the thickness of the sodium polymethacrylate on the surface of the polylactic acid/fertilizer open-cell composite foam material is 20-45nm, the yellow aphrodisiac bacteria are more easily adsorbed.
Preferably, the volume ratio of the water to the 3-aminopropyltriethoxysilane is 100-50: 1.
Preferably, the volume ratio of the dichloromethane, the triethylamine and the 2-bromoisobutyryl bromide is 20-30:1: 1.
Preferably, the volume ratio of the sodium methacrylate to the bipyridyl to the cuprous bromide to the cupric bromide is 100-150:10-15:3-5: 1.
Preferably, the step of sterilizing the polylactic acid/fertilizer open-cell composite foaming material is to expose the yellow plectada carrier to ultraviolet lamp illumination for 1-24 h.
The invention has the beneficial effects that: (1) the polylactic acid/fertilizer open-cell composite foaming material is used as a carrier, fertilizer fine powder is coated in a hole wall in the foaming material, a large amount of yellow aschersonia is adsorbed on the surface of the hole wall, the yellow aschersonia on the surface of the hole wall is released firstly when the polylactic acid/fertilizer open-cell composite foaming material is used, and then the fertilizer fine powder is degraded along with the polylactic acid/fertilizer open-cell composite foaming material to realize controlled release; (2) the grafting of the sodium polymethacrylate can isolate the fine fertilizer powder and the yellow pleopodium parvum, so that the fine fertilizer powder does not influence the yellow pleopodium parvum, and the sodium polymethacrylate film can promote the adsorption of the polylactic acid/fertilizer open-cell composite foaming material on the yellow pleopodium parvum; (3) the used materials are all biodegradable materials, and the soil can not be remained, so that the environment is protected.
Detailed Description
The present invention will be described more clearly and completely with reference to the following specific embodiments, which are obviously only a part of the embodiments of the present invention, but not all of them. All other embodiments, which can be obtained by a person skilled in the art without any inventive step based on the embodiments of the present invention, belong to the scope of the present invention.
Example 1: the preparation method of the solid microbial inoculum for the pine trees comprises the following steps:
(1) drying and grinding fertilizers of 10 parts by weight of urea, 40 parts by weight of diammonium phosphate, 40 parts by weight of potassium chloride and 10 parts by weight of zinc sulfate into fine powder;
(2) melting and mixing polylactic acid and the fine powder fertilizer at 190 ℃ for 1.5h to obtain a polylactic acid/fertilizer composite material;
(3) placing the polylactic acid/fertilizer composite material in a reaction kettle, and introducing carbon dioxide or nitrogen to the pressure of 10 Mpa;
(4) heating the reaction kettle to 80 ℃, and standing for 12 hours;
(5) releasing the pressure of the reaction kettle, cooling in ice bath for 1min to obtain the polylactic acid/fertilizer open-cell composite foam material,
(6) carrying out plasma treatment on the polylactic acid/fertilizer open-cell composite foaming material for 30 s;
(7) grafting sodium methacrylate on the surface of the polylactic acid/fertilizer open-cell composite foam material, and the method comprises the following steps: a) immersing 20g of plasma-treated polylactic acid/fertilizer open-cell composite foaming material in 500ml of water, adding 7g of 3-aminopropyltriethoxysilane under the protection of argon gas, reacting for 12 hours, taking out the foaming material, and drying; b) immersing the dried foaming material in 10ml of dichloromethane and 0.5ml of triethylamine, dripping 0.5ml of 2-bromine isobutyryl bromide under the protection of argon gas for reaction for 12 hours, then taking out the foaming material, sequentially rinsing with water and acetone, and drying; c) adding 5.4g of sodium methacrylate and 0.39g of bipyridyl into 12.5ml of deionized water, standing for 5min until the sodium methacrylate and the bipyridyl are completely dissolved, then adding 0.045g of copper bromide and 0.143g of cuprous bromide, then completely immersing the foam material into the system, reacting for 10h under the protection of argon, then taking out the foam material, and sequentially cleaning with water and methanol to obtain the polylactic acid/fertilizer open-cell composite foam material with the graft thickness of the sodium polymethacrylate being about 40 nm;
(8) placing the yellow aphrodisiac strain into a culture medium, and standing and culturing for 3 days to obtain a yellow aphrodisiac strain liquid;
(9) and (3) mixing and culturing the yellow aschersonia solution and the polylactic acid/fertilizer open-cell composite foaming material which is exposed to ultraviolet lamp illumination for 1h and sterilized at 60 ℃ for 5 days to obtain the yellow aschersonia solid microbial agent.
Example 2: the preparation method of the solid microbial inoculum for the pine trees comprises the following steps:
(1) drying and grinding fertilizers of 15 parts by weight of urea, 40 parts by weight of diammonium phosphate, 30 parts by weight of potassium chloride and 15 parts by weight of zinc sulfate into fine powder;
(2) melting and mixing polylactic acid and the fine powder fertilizer at 200 ℃ for 1h to obtain a polylactic acid/fertilizer composite material;
(3) placing the polylactic acid/fertilizer composite material in a reaction kettle, and introducing carbon dioxide or nitrogen to the pressure of 12 Mpa;
(4) heating the reaction kettle to 100 ℃, and standing for 10 hours;
(5) releasing the pressure of the reaction kettle, and cooling in ice bath for 5min to obtain the polylactic acid/fertilizer open-cell composite foam material;
(6) carrying out plasma treatment on the polylactic acid/fertilizer open-cell composite foaming material for 40 s;
(7) grafting sodium methacrylate on the surface of the polylactic acid/fertilizer open-cell composite foam material, and the method comprises the following steps: a) immersing 30g of plasma-treated polylactic acid/fertilizer open-cell composite foaming material in 800ml of water, adding 14g of 3-aminopropyltriethoxysilane under the protection of argon gas, reacting for 18 hours, taking out the foaming material, and drying; b) immersing the dried foaming material in 20ml of dichloromethane and 1ml of triethylamine, dripping 0.8ml of 2-bromine isobutyryl bromide under the protection of argon gas for reaction for 16 hours, then taking out the foaming material, sequentially rinsing with water and acetone, and drying; c) adding 7g of sodium methacrylate and 0.49g of bipyridyl into 20ml of deionized water, standing for 7min until the sodium methacrylate and the bipyridyl are completely dissolved, then adding 0.063g of cupric bromide and 0.175g of cuprous bromide, then completely immersing the foamed material into the system, reacting for 12h under the protection of argon, then taking out the foamed material, and sequentially washing with water and methanol to obtain the polylactic acid/fertilizer open-cell composite foamed material with the graft thickness of the sodium polymethacrylate of about 60 nm;
(8) placing the yellow aphrodisiac strain into a culture medium, and standing and culturing for 3 days to obtain a yellow aphrodisiac strain liquid;
(9) and (3) mixing and culturing the yellow aschersonia solution and the polylactic acid/fertilizer open-cell composite foaming material which is exposed to ultraviolet lamp illumination for 12 hours and sterilized at 60 ℃ for 5 days to obtain the yellow aschersonia solid microbial agent.
Example 3: the preparation method of the solid microbial inoculum for the pine trees comprises the following steps:
(1) drying and grinding fertilizers of 15 parts by weight of urea, 30 parts by weight of diammonium phosphate, 40 parts by weight of potassium chloride and 15 parts by weight of zinc sulfate into fine powder;
(2) melting and mixing polylactic acid and the fine powder fertilizer at 220 ℃ for 0.5h to obtain a polylactic acid/fertilizer composite material;
(3) placing the polylactic acid/fertilizer composite material in a reaction kettle, and introducing carbon dioxide or nitrogen to the pressure of 15 Mpa;
(4) heating the reaction kettle to 120 ℃, and standing for 6 hours;
(5) releasing the pressure of the reaction kettle, and cooling in ice bath for 3min to obtain the polylactic acid/fertilizer open-cell composite foam material;
(6) carrying out plasma treatment on the polylactic acid/fertilizer open-cell composite foaming material for 60 s;
(7) grafting sodium methacrylate on the surface of the polylactic acid/fertilizer open-cell composite foam material, and the method comprises the following steps: a) immersing 40g of plasma-treated polylactic acid/fertilizer open-cell composite foaming material in 1000ml of water, adding 15g of 3-aminopropyltriethoxysilane under the protection of argon gas, reacting for 24 hours, taking out the foaming material, and drying; b) immersing the dried foaming material in 25ml of dichloromethane and 1.1ml of triethylamine, dripping 1ml of 2-bromine isobutyryl bromide under the protection of argon gas for reaction for 12-24h, then taking out the foaming material, sequentially rinsing with water and acetone, and drying; c) adding 9g of sodium methacrylate and 0.69g of bipyridyl into 30ml of deionized water, standing for 10min until the sodium methacrylate and the bipyridyl are completely dissolved, then adding 0.069g of cupric bromide and 0.215g of cuprous bromide, then completely immersing the foamed material into the system, reacting for 15h under the protection of argon, then taking out the foamed material, and sequentially cleaning with water and methanol to obtain the polylactic acid/fertilizer open-cell composite foamed material with the graft thickness of the sodium polymethacrylate of about 75 nm;
(8) placing the yellow aphrodisiac strain into a culture medium, and standing and culturing for 3 days to obtain a yellow aphrodisiac strain liquid;
(9) and (3) mixing and culturing the yellow aschersonia solution and the polylactic acid/fertilizer open-cell composite foaming material which is exposed to ultraviolet lamp illumination for 24 hours and sterilized at 60 ℃ for 5 days to obtain the yellow aschersonia solid microbial agent.
Table 1: each example shows the amount of adsorbed Vibrio flavus.
Comparative example 1: the difference from example 1 is that in the preparation of the solid microbial inoculum, the grafting is not carried out by using sodium polymethacrylate, and the rest is the same as example 1.
Comparative example 2: the difference from the example 1 is that the grafting reaction time is 5h, the thickness of the sodium polymethacrylate is about 20nm when the solid microbial inoculum is prepared, and the rest is the same as the example 1.
Comparative example 3: the difference from the example 1 is that the grafting reaction time is 15h, the thickness of the sodium polymethacrylate is about 75nm when the solid microbial inoculum is prepared, and the rest is the same as the example 1.
Comparative example 4: the difference from the example 1 is that the grafting reaction time is 30h, the thickness of the sodium polymethacrylate is about 100nm when the solid microbial inoculum is prepared, and the rest is the same as the example 1.
Table 2: different grafting thicknesses of yellow aphrodisiac adsorption capacity.
As shown in Table 2, when the graft thickness of the sodium polymethacrylate is in the range of 40-75nm, the adsorption density of the yellow aphrodisiac bacteria is significantly reduced, and therefore, when the graft thickness of the sodium polymethacrylate is in the range of 40-75nm, the polylactic acid/fertilizer open-cell composite foam material has the best adsorption effect on the yellow aphrodisiac bacteria.
Claims (9)
1. A preparation method of a solid microbial inoculum for pine trees is characterized by comprising the following steps:
(1) drying the fertilizer and grinding into fine powder;
(2) melting and mixing polylactic acid and the fine powder fertilizer at the temperature of 190-220 ℃ for 0.5-1.5h to obtain a polylactic acid/fertilizer composite material;
(3) foaming the polylactic acid/fertilizer composite material to obtain a polylactic acid/fertilizer open-cell composite foam material;
(4) carrying out plasma treatment on the polylactic acid/fertilizer open-cell composite foaming material;
(5) grafting sodium methacrylate on the surface of the polylactic acid/fertilizer open-cell composite foam material;
(6) placing the yellow aphrodisiac strain into a culture medium for standing culture to obtain a yellow aphrodisiac strain liquid;
(7) and mixing and culturing the yellow aschersonia solution and the sterilized polylactic acid/fertilizer open-cell composite foaming material to obtain the yellow aschersonia solid microbial inoculum.
2. The preparation method of the solid microbial inoculum for the pine trees according to claim 1, wherein the fertilizer in the step (1) comprises 10 to 15 parts by weight of urea, 30 to 40 parts by weight of diammonium phosphate, 30 to 40 parts by weight of potassium chloride and 10 to 15 parts by weight of zinc sulfate.
3. The method for preparing the solid microbial inoculum for the pine trees according to claim 1, wherein the step (3) of foaming the polylactic acid/fertilizer composite material comprises the following steps:
(1) placing the polylactic acid/fertilizer composite material in a reaction kettle, and introducing carbon dioxide or nitrogen to the pressure of 8-15 Mpa;
(2) heating the reaction kettle to 80-120 ℃, and standing for 6-12 h;
(3) and (3) releasing the pressure of the reaction kettle, and cooling in ice bath for 1-5min to obtain the polylactic acid/fertilizer open-cell composite foam material.
4. The method for preparing a solid microbial inoculum for pine trees according to claim 3, wherein the pressure relief rate is 25-40 MPa/s.
5. The method for preparing the solid microbial inoculum for the pine trees according to claim 1, wherein the grafting of the sodium methacrylate comprises the following steps:
(1) immersing the polylactic acid/fertilizer open-cell composite foaming material subjected to plasma treatment in water, adding 3-aminopropyltriethoxysilane under the protection of argon, reacting for 12-24h, taking out the foaming material, and drying;
(2) immersing the dried foaming material in 10ml of dichloromethane and 0.5ml of triethylamine, dripping 0.5ml of 2-bromine isobutyryl bromide under the protection of argon gas for reaction for 12-24h, then taking out the foaming material, sequentially rinsing with water and acetone, and drying;
(3) adding sodium methacrylate and bipyridyl into deionized water, standing for 5-10min until the sodium methacrylate and bipyridyl are completely dissolved, then adding cupric bromide and cuprous bromide, completely immersing the foamed material in the system, reacting for 10-15h under the protection of argon, then taking out the foamed material, and sequentially cleaning with water and methanol to obtain the sodium polymethacrylate grafted polylactic acid/fertilizer open-cell composite foamed material.
6. The method for preparing a solid microbial inoculum for pine trees according to claim 5, wherein the grafting thickness of the sodium polymethacrylate is 40-75 nm.
7. The method for preparing a solid microbial inoculum for pine trees according to claim 5, wherein the volume ratio of the water to the 3-aminopropyltriethoxysilane is 100-50: 1.
8. The method for preparing a solid microbial inoculum for pine trees as claimed in claim 7, wherein the volume ratio of the sodium methacrylate, the bipyridyl, the cuprous bromide and the cupric bromide is 100: 10-15:3-5: 1.
9. The method for preparing the solid microbial inoculum for the pine trees according to claim 1, wherein the step of sterilizing the polylactic acid/fertilizer open-cell composite foaming material is to expose the polylactic acid/fertilizer open-cell composite foaming material to ultraviolet lamp illumination for 1-24 h.
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