CN106583431A - Bioremediation method for soil polluted by heavy metal lead cadmium and base on kosteletzkya virginica - Google Patents
Bioremediation method for soil polluted by heavy metal lead cadmium and base on kosteletzkya virginica Download PDFInfo
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- CN106583431A CN106583431A CN201611191050.8A CN201611191050A CN106583431A CN 106583431 A CN106583431 A CN 106583431A CN 201611191050 A CN201611191050 A CN 201611191050A CN 106583431 A CN106583431 A CN 106583431A
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B09—DISPOSAL OF SOLID WASTE; RECLAMATION OF CONTAMINATED SOIL
- B09C—RECLAMATION OF CONTAMINATED SOIL
- B09C1/00—Reclamation of contaminated soil
- B09C1/10—Reclamation of contaminated soil microbiologically, biologically or by using enzymes
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B09—DISPOSAL OF SOLID WASTE; RECLAMATION OF CONTAMINATED SOIL
- B09C—RECLAMATION OF CONTAMINATED SOIL
- B09C1/00—Reclamation of contaminated soil
- B09C1/10—Reclamation of contaminated soil microbiologically, biologically or by using enzymes
- B09C1/105—Reclamation of contaminated soil microbiologically, biologically or by using enzymes using fungi or plants
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Abstract
The invention discloses a bioremediation method for soil polluted by heavy metal lead cadmium and base on kosteletzkya virginica. The bioremediation method comprises the following steps of composite soil preparation, nutrient soil preparation, feeding block preparation, seed pretreatment, sowing, transplantation, fertilization and harvesting. According to the bioremediation method for the soil polluted by the heavy metal lead cadmium and base on the kosteletzkya virginica, the survival rate of the kosteletzkya virginica can be improved, the kosteletzkya virginica grows more robust, and the heavy metal lead cadmium in the soil can be efficiently absorbed; and the absorption rate of the kosteletzkya virginica on the heavy metal lead cadmium is improved by 70% or over compared with the absorption rate of commonly-cultivated kosteletzkya virginica.
Description
Technical field
The invention belongs to heavy metal pollution recovery technique field, and in particular to a kind of heavy metal lead cadmium based on Kosteletzkya virginica
Contaminated soil biological renovation method.
Background technology
Soil is the valuable source that human society is depended on for existence and development, but with industrialized fast development and city
The quickening of change process, China's heavy metal pollution of soil is on the rise.At present, lead cadmium pollution is modal heavy metal pollution of soil
Type, its contaminated soil remediation is a difficulties.Heavy metal lead cadmium pollution is incubation period long, and harm is in chronic accumulation, is difficult
Perceived by people, once and pollute environment, the survival time is long, is just difficult to be removed from environment.
Some for adopting at present either physically or chemically, pollution level heavier soil remediation less for contaminated area
Effect is preferable.But, physically or chemically recovery technique is expensive, it is difficult to large-scale promotion, and is easily destroyed place soil knot
Structure, changes soil physico-chemical property, and may bring secondary pollution.Additionally, phytoremediation has because of low cost, environmental friendliness, row
Imitate and receive significant attention, but, the plant individual repaired currently used for heavy-metal contaminated soil is short and small, poor growth, it is impossible to
Enough Biomass are formed at short notice, causes repairing efficiency longer, so as to limit on a large scale should for phytoremediation technology
With.
Kosteletzkya virginica (Kosteletzkya virginica) is a kind of perennial herb salt tolerant economic plants, belongs to Radix Malvae sylvestriss
Section's Kosteletzkya virginica category, is mainly distributed on the saliferous marshland of subtropical zones, temperate zone and the cool temperature zone in North America.Currently without discovery
Report in terms of Kosteletzkya virginica application and heavy-metal contaminated soil reparation, but repair for heavy-metal contaminated soil relative to other
For multiple plant, Kosteletzkya virginica it is individual tall and big, growth cycle is short, can at short notice form larger Biomass, and
Extremely strong Salt And Alkali Tolerance, resistance to waterlogging, the characteristic of resistance to heavy metal pollution are shown, therefore, trial is applied to heavy-metal contaminated soil
Reparation.
The content of the invention
A kind of heavy metal lead cadmium pollution soil biological renovation method based on Kosteletzkya virginica that the present invention is provided, solves existing
Some heavy metal lead cadmium pollution soil recovery techniques are expensive, repairing efficiency length, be easily destroyed treatment of the earth, change soil
Physicochemical property simultaneously brings the problem of secondary pollution.
The invention provides a kind of heavy metal lead cadmium pollution soil biological renovation method based on Kosteletzkya virginica, including it is as follows
Step:
Step 1, prepares composite soil:According to 3 after river sand, peat, garden mould are smashed to pieces respectively:3:4 mass ratio mixing is equal
It is even, sieve of the mesh diameter for 2mm is crossed, obtain composite soil;
Step 2, prepares Nutrition Soil:Add 25g bush mycorrhiza agents, 20ml phosphorus decomposing microbial inoculums toward every kilogram of composite soil, mixing is equal
It is even, obtain Nutrition Soil;
Step 3, prepares nutritive cube:Nutrition Soil is loaded into external diameter 9cm, in the pot for growing seedlings of high 9cm, nutritive cube is obtained;
Step 4, Seeds preprocess:The good Kosteletzkya virginica seed of full seed, Seed colour is selected, it is soaked in water
Take out two days later, it is standby;
Step 5, sowing:Kosteletzkya virginica seed after soaking in step 4 is seeded in the nutritive cube of step 3, has been sowed
Earthing after finishing, drenches sufficient moisture, and when Kosteletzkya virginica is emerged and launches 2 true leaves, per 5 days, past leaf surface spraying concentration was 0.5mmol/
The EDTA solution of L once, continuously sprays 3 times;
Step 6, transplants:When Kosteletzkya virginica seedling height of seedling reaches 15-20cm, the related mass of soil of seedling is taken from nutritive cube
Go out, be transplanted to by lead, the soil of cadmium pollution;
Step 7, fertilising:Apply bush mycorrhiza agent toward in Kosteletzkya virginica seedling root soil during Kosteletzkya virginica seedling replanting, apply
Dosage is 10-20g/ strains, then applies fertilizer once toward Kosteletzkya virginica root per 20-30 days, and applied amount is 100-200g/ strains,
It is continuous to apply 3 times;
Wherein, the fertilizer is made up of the component of following parts by weight:The ammonium nitrate of 40-50 parts, the di(2-ethylhexyl)phosphate of 10-20 parts
In hydrogen potassium, the lignin sulfonic acid potassium of 10-20 parts, the seaweeds of 1-5 parts, the EDTA- metallo-chelates of 1-3 parts, the richness of 1-3 parts
Acid, the carboxymethyl cellulose of 0.5-1 parts, the dodecylbenzene sodium sulfonate of 0.5-1 parts, the alkyl polyglucoside of 0.5-1 parts,
The growth regulator of 0.05-0.1 parts;
Step 8, harvesting:After Kosteletzkya virginica grows 5-6 month, the part on ground is gathered in;
Step 9,1~step 8 of repeat step, continuation plantation Kosteletzkya virginica heavy metal lead cadmium pollution soil carries out biology and repaiies
It is multiple.
Preferably, the bush mycorrhiza agent is one or two in Glomus etunicatum bacterium or Glomus mosseae bacterium.
Preferably, the phosphorus decomposing microbial inoculum is one or more in bacillus megaterium, Pseudomonas alba, root nodule bacteria.
Preferably, living bacteria count is 1 × 10 in the bush mycorrhiza agent8-2×108Individual/g, in the phosphorus decomposing microbial inoculum effectively
Viable count is 3 × 108-4×108Individual/mL.
Preferably, thickness of earth covering is 0.5-1cm in the step 5.
Preferably, the EDTA- metallo-chelates are EDTA-Fe, EDTA-Ca, EDTA-Mg, one kind in EDTA-Zn or
It is various.
Preferably, the growth regulator is one or more in brassin lactones, gibberellins, naphthalene acetic acid.
Compared with prior art, the beneficial effects of the present invention is:
1) present invention is in nutritive cube by configuring Nutrition Soil sowing Kosteletzkya virginica, on the one hand improve Kosteletzkya virginica into
Motility rate, on the other hand makes Kosteletzkya virginica grow more healthy and strong, is conducive to absorbing the heavy metal lead and cadmium in soil.
2) Radix Malvae sylvestriss by the sea of the invention are transplanted to and are repeatedly applied fertilizer toward root by rear in lead, the soil of cadmium pollution, institute in fertilizer
The ammonium nitrate that contains, potassium dihydrogen phosphate, lignin sulfonic acid potassium can provide the nutrient nitrogen required for its growth to Kosteletzkya virginica
Phosphorus potassium, bush mycorrhiza agent can form symbiosis mycorrhiza with Kosteletzkya virginica, strengthen it to lead, the absorbability of cadmium, EDTA- metal-chelatings
Thing can reduce stability of the heavy metal in soil, so as to strengthen the absorbability of Kosteletzkya virginica heavy metal, fulvic acid one
Aspect can promote absorption of the Kosteletzkya virginica to nutrient, on the other hand make Soil conservation acid, be conducive to strengthening beach brocade
Certain herbaceous plants with big flowers is to lead, the absorption of cadmium.
Specific embodiment
In order that those skilled in the art more fully understand that technical scheme can be practiced, with reference to concrete
The invention will be further described for embodiment, but illustrated embodiment is not as a limitation of the invention.
Strain used is bought in Chinese agriculture Microbiological Culture Collection administrative center, various embodiments of the present invention in the present invention
Described in experimental technique, if no special instructions, be conventional method.
Embodiment 1
A kind of heavy metal lead cadmium pollution soil biological renovation method based on Kosteletzkya virginica, comprises the steps:
Step 1, prepares composite soil:According to 3 after river sand, peat, garden mould are smashed to pieces respectively:3:4 mass ratio mixing is equal
It is even, sieve of the mesh diameter for 2mm is crossed, obtain composite soil;
Step 2, prepares Nutrition Soil:It is 1 × 10 to add living bacteria count toward every kilogram of composite soil8Children's set sacculus of individual/g
Mycete agent 25g, living bacteria count are 3 × 108The bacillus megaterium agent 20ml of individual/mL, mix homogeneously obtains Nutrition Soil;
Step 3, prepares nutritive cube:Nutrition Soil is loaded into external diameter 9cm, in the pot for growing seedlings of high 9cm, nutritive cube is obtained;
Step 4, Seeds preprocess:The good Kosteletzkya virginica seed of full seed, Seed colour is selected, it is soaked in water
Take out two days later, it is standby;
Step 5, sowing:Kosteletzkya virginica seed after soaking in step 4 is seeded in the nutritive cube of step 3, has been sowed
Earthing 0.5cm after finishing, drenches sufficient moisture, when Kosteletzkya virginica is emerged and launches 2 true leaves, is toward leaf surface spraying concentration per 5 days
The EDTA solution of 0.5mmol/L once, continuously sprays 3 times;
Step 6, transplants:When Kosteletzkya virginica seedling height of seedling reaches 15cm, the related mass of soil of seedling is taken out from nutritive cube,
It is transplanted to by lead, the soil of cadmium pollution;
Step 7, fertilising:Applying living bacteria count toward in Kosteletzkya virginica seedling root soil during Kosteletzkya virginica seedling replanting is
1×108The Glomus etunicatum microbial inoculum of individual/g, applied amount is 20g/ strains, then applies fertilizer one toward Kosteletzkya virginica root per 20 days
Secondary, applied amount is 200g/ strains, continuous to apply 3 times;
Wherein, the fertilizer is made up of the component of following parts by weight:50 parts of ammonium nitrate, 15 parts of potassium dihydrogen phosphate,
10 parts of lignin sulfonic acid potassium, 3 parts of seaweeds, 2 parts of EDTA-Fe, 1 part of fulvic acid, 0.5 part of carboxymethyl cellulose, 1
The dodecylbenzene sodium sulfonate, 0.5 part of alkyl polyglucoside, 0.05 part of brassin lactones of part;
Step 8, harvesting:After Kosteletzkya virginica grows 5 months, the part on ground is gathered in;
Step 9,1~step 8 of repeat step, continuation plantation Kosteletzkya virginica heavy metal lead cadmium pollution soil carries out biology and repaiies
It is multiple.
Embodiment 2
A kind of heavy metal lead cadmium pollution soil biological renovation method based on Kosteletzkya virginica, comprises the steps:
Step 1, prepares composite soil:According to 3 after river sand, peat, garden mould are smashed to pieces respectively:3:4 mass ratio mixing is equal
It is even, sieve of the mesh diameter for 2mm is crossed, obtain composite soil;
Step 2, prepares Nutrition Soil:It is 1.5 × 10 to add living bacteria count toward every kilogram of composite soil8Children's set ball of individual/g
Capsule mycete agent 25g, living bacteria count are 3.5 × 108The Pseudomonas alba agent 20ml of individual/mL, mix homogeneously obtains Nutrition Soil;
Step 3, prepares nutritive cube:Nutrition Soil is loaded into external diameter 9cm, in the pot for growing seedlings of high 9cm, nutritive cube is obtained;
Step 4, Seeds preprocess:The good Kosteletzkya virginica seed of full seed, Seed colour is selected, it is soaked in water
Take out two days later, it is standby;
Step 5, sowing:Kosteletzkya virginica seed after soaking in step 4 is seeded in the nutritive cube of step 3, has been sowed
Earthing 1cm after finishing, drenches sufficient moisture, when Kosteletzkya virginica is emerged and launches 2 true leaves, is toward leaf surface spraying concentration per 5 days
The EDTA solution of 0.5mmol/L once, continuously sprays 3 times;
Step 6, transplants:When Kosteletzkya virginica seedling height of seedling reaches 18cm, the related mass of soil of seedling is taken out from nutritive cube,
It is transplanted to by lead, the soil of cadmium pollution;
Step 7, fertilising:During Kosteletzkya virginica seedling replanting toward in Kosteletzkya virginica seedling root soil apply Glomus etunicatum bacterium
Agent, applied amount is 15g/ strains, then applies fertilizer once toward Kosteletzkya virginica root per 25 days, and applied amount is 150g/ strains, is continuously applied
Plus 3 times;
Wherein, the fertilizer is made up of the component of following parts by weight:45 parts of ammonium nitrate, 20 parts of potassium dihydrogen phosphate,
15 parts of lignin sulfonic acid potassium, 5 parts of seaweeds, 3 parts of EDTA-Ca, 3 parts of fulvic acid, 1 part of carboxymethyl cellulose, 0.5
The dodecylbenzene sodium sulfonate, 1 part of alkyl polyglucoside, 0.1 part of gibberellins of part;
Step 8, harvesting:After Kosteletzkya virginica grows 6 months, the part on ground is gathered in;
Step 9,1~step 8 of repeat step, continuation plantation Kosteletzkya virginica heavy metal lead cadmium pollution soil carries out biology and repaiies
It is multiple.
Embodiment 3
A kind of heavy metal lead cadmium pollution soil biological renovation method based on Kosteletzkya virginica, comprises the steps:
Step 1, prepares composite soil:According to 3 after river sand, peat, garden mould are smashed to pieces respectively:3:4 mass ratio mixing is equal
It is even, sieve of the mesh diameter for 2mm is crossed, obtain composite soil;
Step 2, prepares Nutrition Soil:It is 2 × 10 to add living bacteria count toward every kilogram of composite soil8Moses's sacculus of individual/g
Mycete agent 25g, living bacteria count are 4 × 108The nitragin 20ml of individual/mL, mix homogeneously obtains Nutrition Soil;
Step 3, prepares nutritive cube:Nutrition Soil is loaded into external diameter 9cm, in the pot for growing seedlings of high 9cm, nutritive cube is obtained;
Step 4, Seeds preprocess:The good Kosteletzkya virginica seed of full seed, Seed colour is selected, it is soaked in water
Take out two days later, it is standby;
Step 5, sowing:Kosteletzkya virginica seed after soaking in step 4 is seeded in the nutritive cube of step 3, has been sowed
Earthing 0.8cm after finishing, drenches sufficient moisture, when Kosteletzkya virginica is emerged and launches 2 true leaves, is toward leaf surface spraying concentration per 5 days
The EDTA solution of 0.5mmol/L once, continuously sprays 3 times;
Step 6, transplants:When Kosteletzkya virginica seedling height of seedling reaches 20cm, the related mass of soil of seedling is taken out from nutritive cube,
It is transplanted to by lead, the soil of cadmium pollution;
Step 7, fertilising:During Kosteletzkya virginica seedling replanting toward in Kosteletzkya virginica seedling root soil apply Glomus mosseae bacterium
Agent, applied amount is 10g/ strains, then applies fertilizer once toward Kosteletzkya virginica root per 30 days, and applied amount is 100g/ strains, is continuously applied
Plus 3 times;
Wherein, the fertilizer is made up of the component of following parts by weight:40 parts of ammonium nitrate, 20 parts of potassium dihydrogen phosphate,
20 parts of lignin sulfonic acid potassium, 1 part of seaweeds, 1 part of EDTA-Mg, 2 parts of fulvic acid, 1 part of carboxymethyl cellulose, 1 part
Dodecylbenzene sodium sulfonate, 1 part of alkyl polyglucoside, 0.08 part of naphthalene acetic acid;
Step 8, harvesting:After Kosteletzkya virginica grows 6 months, the part on ground is gathered in;
Step 9,1~step 8 of repeat step, continuation plantation Kosteletzkya virginica heavy metal lead cadmium pollution soil carries out biology and repaiies
It is multiple.
The Kosteletzkya virginica that the method that embodiment 1-3 is adopted is planted out can be carried out very to the heavy metal lead in soil, cadmium
Good absorption, after plantation harvesting for the first time lead, cadmium concentration in the Kosteletzkya virginica for gathering in is detected, specific experiment result is as follows:
Lead, the cadmium concentration absorbed in the Kosteletzkya virginica of table 1
Cadmium content (mg/kg) in Kosteletzkya virginica | Lead content (mg/kg) in Kosteletzkya virginica | |
Embodiment 1 | 163.36 | 630.93 |
Embodiment 2 | 175.44 | 658.32 |
Embodiment 3 | 181.52 | 703.13 |
Embodiment 1-3 is after by the sea Radix Malvae sylvestriss harvest, along native face clip aerial partss, to weigh after 105 DEG C of drying dry
Weight, is then disappeared with nitric acid-perchloric acid method and is boiled, then determines the concentration of lead, cadmium using ICP.As it can be seen from table 1 the inventive method
The Kosteletzkya virginica planted out had absorption well to the heavy metal lead and cadmium in contaminated soil, through the trophophase of 5-6 month
Afterwards, absorption of the Kosteletzkya virginica to the cadmium in soil can reach more than 160mg/kg, and the absorption to lead can reach 630mg/kg
More than, for prior art, absorbance improves more than 70%.
Present invention configuration Nutrition Soil, and Nutrition Soil is fitted in pot for growing seedlings is configured to nutritive cube to sow Kosteletzkya virginica, make
The survival rate of Kosteletzkya virginica to 100%, far superior to traditional direct survival rate that Kosteletzkya virginica is sowed on contaminated land
(about 70%), and due to just providing enough nutrition, Kosteletzkya virginica life to Kosteletzkya virginica in seed stage and seedling stage
Grow more healthy and strong, be more able to absorb the heavy metal lead and cadmium in soil.
Radix Malvae sylvestriss by the sea of the invention are transplanted to and are repeatedly applied fertilizer toward root by rear in lead, the soil of cadmium pollution, contained in fertilizer
Ammonium nitrate, potassium dihydrogen phosphate, lignin sulfonic acid potassium can to Kosteletzkya virginica provide its growth required for nutrient nitrogen phosphorus
Potassium, meanwhile, lignin sulfonic acid potassium can also improved soil structure, chesson, improve soil fertilizer-preserving ability, adjust pH value,
The content of heavy metal in soil is reduced, harm of the salt ion to Kosteletzkya virginica seedling is reduced;Bush mycorrhiza agent can and Kosteletzkya virginica
Symbiosis mycorrhiza is formed, strengthens it to lead, the absorbability of cadmium, EDTA- metallo-chelates can reduce heavy metal in soil
Stability, so as to strengthen the absorbability of Kosteletzkya virginica heavy metal, on the one hand fulvic acid can promote Kosteletzkya virginica to nutrition
The absorption of element, on the other hand makes Soil conservation acid, is conducive to strengthening Kosteletzkya virginica to lead, the absorption of cadmium;The side of seaweeds one
Face with fulvic acid synergism, can keep the acidity of soil, on the other hand can promote plant cell division, promote Kosteletzkya virginica root
The growth of stem;Dodecylbenzene sodium sulfonate is a kind of anion surface active base, and alkyl polyglucoside is a kind of non-ionic surface
Active group, the two can make heavy metal be more conducive to complexation or by Kosteletzkya virginica root with synergism to reduce soil surface tension force
System absorbs, meanwhile, the amphipathic of surfactant makes it to be interacted with the hydrophilic and lipophilic group in plant cell membrane, from
And the structure and permeability of change cell membrane, promote the absorption of plant heavy metal.
, but those skilled in the art once know basic creation although preferred embodiments of the present invention have been described
Property concept, then can make other change and modification to these embodiments.So, claims are intended to be construed to include excellent
Select embodiment and fall into having altered and changing for the scope of the invention.
Obviously, those skilled in the art can carry out the essence of various changes and modification without deviating from the present invention to the present invention
God and scope.So, if these modifications of the present invention and modification belong to the scope of the claims in the present invention and its equivalent technologies
Within be also intended to comprising these change and modification.
Claims (7)
1. a kind of heavy metal lead cadmium pollution soil biological renovation method based on Kosteletzkya virginica, it is characterised in that including following step
Suddenly:
Step 1, prepares composite soil:According to 3 after river sand, peat, garden mould are smashed to pieces respectively:3:4 quality is than mix homogeneously, mistake
Mesh diameter is the sieve of 2mm, obtains composite soil;
Step 2, prepares Nutrition Soil:Add 25g bush mycorrhiza agents, 20ml phosphorus decomposing microbial inoculums toward every kilogram of composite soil, mix homogeneously is obtained
To Nutrition Soil;
Step 3, prepares nutritive cube:Nutrition Soil is loaded into external diameter 9cm, in the pot for growing seedlings of high 9cm, nutritive cube is obtained;
Step 4, Seeds preprocess:The good Kosteletzkya virginica seed of full seed, Seed colour is selected, it is soaked two days in water
After take out, it is standby;
Step 5, sowing:Kosteletzkya virginica seed after soaking in step 4 is seeded in the nutritive cube of step 3, after sowing is finished
Earthing, drenches sufficient moisture, when Kosteletzkya virginica is emerged and launches 2 true leaves, per 5 days toward leaf surface spraying concentration for 0.5mmol/L's
EDTA solution once, continuously sprays 3 times;
Step 6, transplants:When Kosteletzkya virginica seedling height of seedling reaches 15-20cm, the related mass of soil of seedling is taken out from nutritive cube,
It is transplanted to by lead, the soil of cadmium pollution;
Step 7, fertilising:Apply bush mycorrhiza agent, applied amount toward in Kosteletzkya virginica seedling root soil during Kosteletzkya virginica seedling replanting
For 10-20g/ strains, then applied fertilizer once toward Kosteletzkya virginica root per 20-30 days, applied amount is 100-200g/ strains, continuously
Apply 3 times;
Wherein, the fertilizer is made up of the component of following parts by weight:The ammonium nitrate of 40-50 parts, the biphosphate of 10-20 parts
Potassium, the lignin sulfonic acid potassium of 10-20 parts, the seaweeds of 1-5 parts, the EDTA- metallo-chelates of 1-3 parts, the fulvic acid of 1-3 parts,
The carboxymethyl cellulose of 0.5-1 parts, the dodecylbenzene sodium sulfonate of 0.5-1 parts, the alkyl polyglucoside of 0.5-1 parts, 0.05-
0.1 part of growth regulator;
Step 8, harvesting:After Kosteletzkya virginica grows 5-6 month, the part on ground is gathered in;
Step 9,1~step 8 of repeat step, continuing plantation Kosteletzkya virginica heavy metal lead cadmium pollution soil carries out biological restoration.
2. the heavy metal lead cadmium pollution soil biological renovation method based on Kosteletzkya virginica according to claim 1, its feature
It is that the bush mycorrhiza agent is Glomus etunicatum bacterium (Glomus etunicatum) or Glomus mosseae bacterium (Glomus
Mosseae one or two in).
3. the heavy metal lead cadmium pollution soil biological renovation method based on Kosteletzkya virginica according to claim 1, its feature
It is that the phosphorus decomposing microbial inoculum is bacillus megaterium (Bacillus megaterium), Pseudomonas alba
(Pseudomonadaceae), one or more in root nodule bacteria (Root nodule bacteria).
4. the heavy metal lead cadmium pollution soil biological renovation method based on Kosteletzkya virginica according to claim 1, its feature
It is that living bacteria count is 1 × 10 in the bush mycorrhiza agent8-2×108Individual/g, in the phosphorus decomposing microbial inoculum living bacteria count be 3 ×
108-4×108Individual/mL.
5. the heavy metal lead cadmium pollution soil biological renovation method based on Kosteletzkya virginica according to claim 1, its feature
It is that thickness of earth covering is 0.5-1cm in the step 5.
6. the heavy metal lead cadmium pollution soil biological renovation method based on Kosteletzkya virginica according to claim 1, its feature
Be, the EDTA- metallo-chelates be EDTA-Fe, EDTA-Ca, EDTA-Mg, one or more in EDTA-Zn.
7. the heavy metal lead cadmium pollution soil biological renovation method based on Kosteletzkya virginica according to claim 1, its feature
It is that the growth regulator is one or more in brassin lactones, gibberellins, naphthalene acetic acid.
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CN107155672A (en) * | 2017-07-03 | 2017-09-15 | 中国计量大学 | Method for adjusting and controlling rice heavy metal cadmium accumulation |
CN107278654A (en) * | 2017-07-03 | 2017-10-24 | 中国计量大学 | Promote the method for paddy rice stem heavy metal cadmium accumulation and its rice plant of preparation using 24 Epibrassinolides |
CN107127209A (en) * | 2017-07-17 | 2017-09-05 | 中国环境科学研究院 | A kind of method of antimicrobial plant renovation of heavy metal polluted soil with combined |
CN107671114A (en) * | 2017-11-22 | 2018-02-09 | 新乡学院 | A kind of heavy metal lead cadmium pollution soil biological renovation method based on plant |
CN107980266A (en) * | 2017-12-09 | 2018-05-04 | 湖南科技大学 | Suppress the method for brassica plant absorption cadmium using the bacterium for producing ABA |
CN108605706A (en) * | 2018-03-20 | 2018-10-02 | 中山大学 | A method of promoting tomato tolerant ability to cadmium |
CN109226219A (en) * | 2018-10-17 | 2019-01-18 | 常州大学 | A kind of method that nitrogenous fertilizer strengthens alternanthera philoxeroides cadmium pollution soil repair |
CN111011155A (en) * | 2019-12-19 | 2020-04-17 | 上海市园林科学规划研究院 | Special compound soil for magnolia denudata and preparation method and application thereof |
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