CN109157470A - A kind of pox-dispelling function whitening skin-protection cream and preparation method thereof - Google Patents
A kind of pox-dispelling function whitening skin-protection cream and preparation method thereof Download PDFInfo
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Abstract
The invention discloses a kind of pox-dispelling function whitening skin-protection creams and preparation method thereof.The face cream includes following components: glycerol, tocopherol, phytosterol/octyl dodecanol lauroyl glutamate ester, stearine, cholesterine polyethers -24, docosyl alcohol, stearyl alcohol glycyrrhetin acid esters, blue gum leaf oil, undecylenoyl phenylalanine, Radix Aristolochiae extract, ceriops tagal extract, Rhizoma Anemones Altaicae extract, contain researching advances on growth factor liposomes, plants fruits and seeds superfine powder, sodium acrylate/sodium acryloyldimethyl taurate copolymers, Phenoxyethanol, Sensiva SC50 at hydrogenated coco, and surplus is deionized water.The effect of face cream contains a variety of active skull cap components, can effectively penetrate into skin histology, and mutually coordinated performance improves acne flesh, inhibits small pox regeneration, smooths acne hole, desalinate acne print, there is good therapeutic effect to whelk, while there is fine whitening effect.
Description
Technical field
The invention belongs to cosmetic technical fields, and in particular to a kind of pox-dispelling function whitening skin-protection cream and preparation method thereof.
Background technique
Acne is commonly called as whelk, dark sore, acne, is a kind of chronic inflammatory skin of pilosebaceous follicle, main to occur
At the position that face, shirtfront, omoplate smegma are vigorous.The generation of whelk mainly with hypersteatosis, hair follicle sebum
The factors such as gland catheter blockage, bacterium infection and inflammatory reaction are closely related.Adolesce androgen especially testis in descendant's body
The level of ketone increases rapidly, and sebaceous glands is promoted to develop and generate a large amount of sebums.The dyskeratosis of pilosebaceous duct is made simultaneously
At catheter blockage, dyssebacia forms horn plug, that is, micro mist thorn.Multiple-microorganism especially propionibacterium acnes in hair follicle
Mass propagation, the lipase that propionibacterium acnes generate decompose sebum and generate free fatty acid, while chemotactic inflammatory cell and medium,
Finally induces and aggravate inflammatory reaction.Whelk seriously affects the appearance and confidence of people, and it is passive to generate to work, the life of people
It influences, therefore anti-acne is one of research focus of cosmetics.
There are many kinds of the methods of existing anti-acne, 1, such as laser therapy whelk method, can rapid damage propionic acid whelk
Bacillus, effectively inhibition bacterial reproduction, eliminate its stimulation and destruction to tissue, but the effect of laser therapy is too strong, and
And only physical method destroys acne, it cannot fundamentally anti-acne；2, some using aloe, chamomile, tea tree, fructus hippophae etc.
Natural plant component, Chinese medicinal ingredients or Chinese medicine and plant mixing composition anti-acne, existing this anti-acne method safety, Neng Gouqi
To adjusting skin oil and fat to the effect of fundamentally anti-acne, but it is long using time when this natral plant ingredients anti-acne, dispel
The marking after acne is not easy to completely remove, and is formulated to be improved；3, some Western medicine such as salicylic acid, Bo Nisong, dexamethasone are utilized
Except acne, the side effect of Western medicine is strong, can destroy skin normal flora, at high cost.Therefore, it is necessary to research and develop a kind of effectively conditioning acne
Flesh inhibits small pox regeneration, smooths acne hole, repairs damaged cell tissue, effectively remove acne print, do not stimulate mildly, muting sensitive safety
Pox-dispelling function whitening and skin-protecting product.
Summary of the invention
The object of the present invention is to provide a kind of pox-dispelling function whitening skin-protection creams and preparation method thereof.The face cream contains
A variety of active skull cap components can effectively penetrate into skin histology, and mutually coordinated performance improves acne flesh, inhibit small pox regeneration, smooth
The effect of acne hole, desalination acne print, has good therapeutic effect to whelk, while having fine whitening function.
The present invention is achieved by the following technical programs:
A kind of pox-dispelling function whitening skin-protection cream, by mass percentage, including following components: glycerol 2~6%, tocopherol 1
~3%, phytosterol/octyl dodecanol lauroyl glutamate ester 0.5~1.5%, hydrogenated coco 1~2%, stearine
1~2%, cholesterine polyethers -24 1~3%, docosyl alcohol 0.5~2%, stearyl alcohol glycyrrhetin acid esters 1~2%, blue gum leaf oil 0.5
~1.5%, undecylenoyl phenylalanine 1~3%, Radix Aristolochiae extract 1~4%, ceriops tagal extract 1~3%, nine sections
Calamus extract 1~4% contains researching advances on growth factor liposomes 8~12%, plants fruits and seeds superfine powder 6~8%, sodium acrylate/acryloyl
Dimethyltaurine sodium copolymer 0.5~1.5%, Phenoxyethanol 0.05~0.1%, Sensiva SC50 0.05~0.1%, it is remaining
Amount is deionized water.
Described contains researching advances on growth factor liposomes, includes following components: Anji white tea callus tissue culture according to parts by weight
10~20 parts of object filtrate, 0.00005~0.001 part of recombinant human horny cell growth factor-2,1~3 part of Vitexin, hydrogenated soybean ovum
5~45 parts of phosphatidase 3,5~15 parts of cholesterol, 5~15 parts of cupreol and 4~7 parts of Tween-80.
It is preferred that described contains researching advances on growth factor liposomes, it include following components: Anji white tea callus group according to parts by weight
Knit 15 parts of culture filtrate, 0.0001 part of recombinant human horny cell growth factor-2,2 parts of Vitexin, 40 parts of hydrogenated soy phosphatidyl choline,
10 parts of cholesterol, 5 parts of cupreol and 5 parts of Tween-80.
The preparation containing researching advances on growth factor liposomes the following steps are included:
(1) Vitexin, hydrogenated soy phosphatidyl choline, cholesterol, cupreol and the Tween-80 of formula ratio are weighed, mixes, adds
Enter dehydrated alcohol, dissolution is sufficiently stirred, obtains solution A；
(2) recombinant human horny cell growth factor-2 is dissolved in the PBS buffer solution that pH is 7.2, stirring is made to being completely dissolved
At the solution of 0.1~0.2mg/mL, the Anji white tea callus tissue culture filtrate of formula ratio is then added, is uniformly mixed, obtains
To solution B；
(3) in the case where magnetic stirring apparatus is stirred continuously, solution B is injected into solution A with syringe, it is sufficiently mixed 0.5~
1h, then at 28~32 DEG C, rotary evaporation under conditions of 0.1~1MPa removes ethyl alcohol, then vibrates water in 30~35 DEG C of water-baths
Change 1~2h of reaction, obtain liposome suspension, using the partial size of syringe type filter adjustment liposome suspension to 100~
150nm is to get containing researching advances on growth factor liposomes.
The plants fruits and seeds superfine powder is moringa seeds, Semen Vitis Amurensis and buckwheat seed with (1~2): (0.5~1): (1~3)
Mass ratio composition.
It is preferred that the plants fruits and seeds superfine powder is moringa seeds, Semen Vitis Amurensis and buckwheat seed with the mass ratio of 1.5:1:2.5
Composition.
The preparation of the plants fruits and seeds superfine powder is the following steps are included: by the moringa seeds, Semen Vitis Amurensis and buckwheat of formula ratio
Wheat seed mixes, and crushes, obtains seed mixed-powder；Seed mixed-powder is added in airflow milling powder machine, airflow milling is carried out,
The micro mist sufficiently ground is with air-flow by the gap of separation wheel, and what is do not ground sufficiently cannot then pass through, and continue
Airflow milling, until the partial size of powder is 0.2~1 μm, collection obtains plants fruits and seeds superfine powder.
The present invention also provides a kind of preparation methods of above-mentioned pox-dispelling function whitening skin-protection cream comprising following steps:
(1) by the deionized water of formula ratio, glycerol, undecylenoyl phenylalanine, plants fruits and seeds superfine powder and propylene
Sour sodium/sodium acryloyldimethyl taurate copolymers are added water phase pot, are heated to 70~75 DEG C, stir evenly, obtain water phase；
(2) tocopherol of formula ratio, phytosterol/octyl dodecanol lauroyl glutamate ester, hydrogenated coco, glycerol is hard
Oily phase pot is added in resin acid ester, cholesterine polyethers -24, docosyl alcohol, stearyl alcohol glycyrrhetin acid esters and blue gum leaf oil, it is heated to 70~
It 75 DEG C, stirs evenly, obtains oily phase；
(3) will water phase be pumped into vacuum emulsifying machine in, open stirring and vacuum, 80~85 DEG C, vacuum degree be -0.06~-
Under conditions of 0.04MPa, speed of agitator are 60~80r/min, by oily mutually sucking mulser, mixed liquor are obtained, is then existed
10~15min of homogeneous under 2000~3000r/min；
(4) it after the completion of homogeneous, keeps vacuum degree and mixing speed constant, is cooled to 40~45 DEG C, dutchmanspipe root is added and extracts
Object, Rhizoma Anemones Altaicae extract, contains researching advances on growth factor liposomes, Phenoxyethanol and Sensiva SC50 at ceriops tagal extract, continues to stir
It mixes uniformly, is cooled to room temperature, discharge, it is filling to get pox-dispelling function whitening skin-protection cream.
Pox-dispelling function whitening skin-protection cream provided by the invention is with stearyl alcohol glycyrrhetin acid esters, blue gum leaf oil, endecatylene acyl
Base phenylalanine, Radix Aristolochiae extract, ceriops tagal extract, Rhizoma Anemones Altaicae extract and the conduct containing researching advances on growth factor liposomes are main
Effective component inhibits the inflammation of skin by inhibiting the activity of staphylococcus aureus, staphylococcus epidermis and propionibacterium acnes
Disease reaction；By inhibiting the activity of 5- alpha-reductase to adjust the activity of hormone, inhibits the secretion of sebaceous glands, mitigate and eliminate Cuo
The generation of sore；By promoting cutin to fall off, old useless cutin is removed, pore channel is opened, defends pilosebaceous duct exception angle
Change the micro mist thorn formed and acne block pore；By removing free radical, promoting skin collagen matter, elastomer and other vigor
The regeneration of protein plays significant skin repair ability, and damaged cell tissue is made to restore cell viability, is conducive to smooth acne hole,
Desalinate acne print.Finally make face cream obtained that there is significant pox-dispelling function whitening effect.
Radix Aristolochiae extract in formula has the bactericidal activity of wide spectrum, to staphylococcus aureus, staphylococcus epidermis
There is stronger killing effect with propionibacterium acnes, the inflammatory reaction for effectively inhibiting above-mentioned pathogenic bacteria to cause.Ceriops tagal extracts
Object has stronger astriction, inhibits sebum secretion, compresses tissue, pore refining, may advantageously facilitate the healing of wound and disappear
Purulence.Rhizoma Anemones Altaicae extract itself contains a certain amount of catalase (CAT), and can enhance superoxide dismutase (SOD)
Activity, stablize target cell membrane, scavenging effect all had to hydroxy radical, hydrogen peroxide, singlet oxygen, to desalination acne print, extensive
It is bright with positive effect that skin and muscles are fair again.The undecylenoyl phenylalanine passes through competitive binding melanocyte
Upper melanin stimulating hormone receptor MC1-R, makes melanocyte that cannot generate tyrosinase, thus inhibit melanocyte activity,
The generation for reducing melanin can enhance the effect that Rhizoma Anemones Altaicae extract eliminates acne print.Stearyl alcohol glycyrrhetin acid esters, which has, releives
Antiallergic effect, effectively soothes the skin the senses of discomfort such as redness, inflammation.Blue gum leaf oil can enhance the bactericidal activity of Radix Aristolochiae extract,
And can promote blood circulation, promote skin cuticula to update, is conducive to remove old useless cutin, opens pore channel, defend hair follicle
The micro mist thorn and acne that sebaceous gland duct dyskeratosis is formed block pore.
In addition, described contains Anji white tea callus tissue culture filtrate, recombined human cutin containing researching advances on growth factor liposomes
Three kinds of effective active compositions of Porcine HGF and Vitexin can further enhance anti-acne effect, promote skin repair, smooth acne
Acne print is desalinated in hole.Wherein, Anji white tea callus tissue culture filtrate, unique growing environment assign its unique life system
System, the active nutrient carried has significant free radical scavenging ability, and can act on epidermis and skin corium simultaneously, helps
The regeneration of cell protein promotes the generation and building of extracellular matrix, is conducive to smooth acne hole, in addition, containing in its filtrate
A large amount of tea polyphenols reduce movable sebaceous glands quantity and skin by inhibiting the activity of 5- alpha-reductase to adjust the activity of hormone
Seborrhea flow mitigates and eliminates the generation of acne to achieve the effect that effectively to adjust fat secretion.Recombination human body keratinized cell growth
The factor can obvious stimulation people's maturation horn cell growth, repair horny layer of epidermis, have moisturizing, anti-inflammatory effect, can be significant
Reinforce the ability that skin resists extraneous pessimal stimulation, is no longer easy to infect small pox after making cytothesis reparation.Vitexin is day
Right flavone compound prevents melanin pigmentation, simultaneously because it is with good antioxygen by inhibiting tyrosine kinase activity
The property changed, it is possible to reduce the injury of free radical, the effect of playing whitening.
Plants fruits and seeds superfine powder in formula is the mixture of moringa seeds, Semen Vitis Amurensis and buckwheat seed superfine powder, be can be improved
The smooth effect of pox-dispelling function whitening skin-protection cream improves salubrious degree, prevents clogging of pores, prevent the formation of fat granule.
Pox-dispelling function whitening skin-protection cream appearance provided by the invention is fine and smooth lotion, and pH is 6.2~7.1, viscosity 10000
~15000mpa.s, 45 DEG C 24 hours heat-resisting, changes after restoring room temperature without obvious character, and -18 DEG C 24 hours cold-resistant, restores room temperature
Change afterwards without obvious character.
Ingredient in inventive formulation is unless otherwise instructed commercial product.Wherein, Radix Aristolochiae extract, ceriops tagal
Extract, Rhizoma Anemones Altaicae extract are purchased from Xi'an Bioisystech Co., Ltd of Tianrui.Blue gum leaf oil is refined purchased from the bright Ji in Guangzhou
Trade Co., Ltd.Stearyl alcohol glycyrrhetin acid esters CAS are as follows: 13832-70-7 is purchased from Hubei Ju Sheng Science and Technology Ltd..Recombination
Human horny cell growth factor-2 CAS are as follows: 148348-15-6 is purchased from Hubei Guang Ao Biotechnology Co., Ltd.Vitexin CAS
Number are as follows: 3681-93-4 is purchased from Shanghai Hui Cheng Biotechnology Co., Ltd.No. CAS of undecylenoyl phenylalanine are as follows:
175357-18-3 is purchased from Xi'an Jia Boying Biotechnology Co., Ltd.
Moringa seeds are purchased from Guangdong Sheng Kang pharmaceutcal corporation, Ltd；Semen Vitis Amurensis is public purchased from the good boat medicine company Limited Liability of Bozhou City
Department；Buckwheat seed sells Co., Ltd purchased from the benevalent hall Chinese medicine of Bozhou City.
The Anji white tea callus tissue culture filtrate is made by following steps:
(1) using the blade of Anji white tea as the explant of evoked callus, on superclean bench, first with 70% second
Alcoholic solution impregnates 5min, then with aseptic water washing 3~5 times, blots surface moisture with sterilizing filter paper, be cut into 0.3cm × 0.3cm's
Fritter, is inoculated in the solid medium of MS+1~2mg/L TDZ+4g/L agar and carries out callus induction, and condition of culture is
25 ± 2 DEG C of temperature, light application time 12h/d, 30~40mmoLm of intensity of illumination-2·s-1；Cultivation cycle is 45 days；
(2) the good callus of growth conditions for taking step (1) to obtain, with sterilized tweezers on superclean bench
It is divided into mung bean size, is seeded to MS+0.5 containing 40mL~4mg/L TDZ+0.5~4mg/L IAA, the liquid that pH is 5.8
In the 100mL conical flask of body culture medium, on 25 ± 2 DEG C of constant-temperature table, 100rpm, light application time 12h/d, intensity of illumination 30
~40mmoLm-2·s-1Under the conditions of carry out suspension culture, after cultivating 40~45d, take filtrate after being sieved through filter with 50 aim cells,
Filtrate uses 80 aim cells to be sieved through filter again, takes filtrate, sterilizes to get Anji white tea callus tissue culture filtrate.
Compared with prior art, the invention has the following advantages:
(1) pox-dispelling function whitening skin-protection cream of the present invention contains a variety of active skull cap components, can effectively penetrate into skin histology,
The effect of mutually coordinated performance improves acne flesh, inhibits small pox regeneration, smooths acne hole, desalinate acne print, has whelk good
Therapeutic effect, while there is fine whitening effect.
(2) pox-dispelling function whitening skin-protection cream of the present invention do not stimulate mildly, easily absorption, non-greasy, safe and efficient, and prepare
Process stabilizing, it is quality controllable.
Specific embodiment
The following examples are further illustrations of the invention, rather than limiting the invention.
The preparation of 1 Anji white tea callus tissue culture filtrate of embodiment
Anji white tea callus tissue culture filtrate is made by following steps:
(1) using the blade of Anji white tea as the explant of evoked callus, on superclean bench, first with 70% second
Alcoholic solution impregnates 5min, then with aseptic water washing 3~5 times, blots surface moisture with sterilizing filter paper, be cut into 0.3cm × 0.3cm's
Fritter is inoculated in the solid medium of MS+2mg/L TDZ+4g/L agar and carries out callus induction, and condition of culture is temperature
27 DEG C, light application time 12h/d, intensity of illumination 30mmoLm-2·s-1；Cultivation cycle is 45 days；
(2) the good callus of growth conditions for taking step (1) to obtain, with sterilized tweezers on superclean bench
It is divided into mung bean size, is seeded to the TDZ+2mg/L of MS+2mg/L containing 40mL IAA, the fluid nutrient medium that pH is 5.8
In 100mL conical flask, on 27 DEG C of constant-temperature table, 100rpm, light application time 12h/d, intensity of illumination 30mmoLm-2·s-1
Under the conditions of carry out suspension culture, after cultivating 45d, filtrate is taken after being sieved through filter with 50 aim cells, filtrate uses 80 aim cells to sieve again
Filtering takes filtrate, sterilizes to get Anji white tea callus tissue culture filtrate.
The preparation of 2 plants fruits and seeds superfine powder of embodiment
The formula composition of 2 plants fruits and seeds superfine powder of embodiment are as follows: based on 100g plants fruits and seeds superfine powder, including shown in following table
Component:
| Component | A group | B group | C group |
| Moringa seeds | 30g | 33.3g | 40g |
| Semen Vitis Amurensis | 20g | 16.7g | 20g |
| Buckwheat seed | 50g | 50g | 40g |
Preparation method: the moringa seeds of formula ratio, Semen Vitis Amurensis and buckwheat seed mix, crushes, obtains seed mixed powder
End；Seed mixed-powder is added in airflow milling powder machine, carries out airflow milling, the micro mist sufficiently ground is with air-flow by dividing
The gap of wheel is selected, and what is do not ground sufficiently cannot then pass through, and continue airflow milling, until the partial size of powder is 0.2~1 μ
M, collection obtain plants fruits and seeds superfine powder.
Embodiment 3 is prepared containing researching advances on growth factor liposomes
Formula composition of the embodiment 3 containing researching advances on growth factor liposomes includes component described in the following table 1 according to parts by weight:
Formula composition of the table 1 containing researching advances on growth factor liposomes
Be formulated 1 preparation containing researching advances on growth factor liposomes the following steps are included:
(1) Vitexin, hydrogenated soy phosphatidyl choline, cholesterol, cupreol and the Tween-80 of formula ratio are weighed, mixes, adds
Enter dehydrated alcohol, dissolution is sufficiently stirred, obtains solution A；
(2) recombinant human horny cell growth factor-2 is dissolved in the PBS buffer solution that pH is 7.2, stirring is made to being completely dissolved
At the solution of 0.1~0.2mg/mL, the Anji white tea callus tissue culture filtrate of formula ratio is then added, is uniformly mixed, obtains
To solution B；
(3) in the case where magnetic stirring apparatus is stirred continuously, solution B is injected into solution A with syringe, is sufficiently mixed 1h, so
Afterwards at 32 DEG C, rotary evaporation under conditions of 1MPa removes ethyl alcohol, then hydration reaction 2h is vibrated in 30 DEG C of water-baths, obtains liposome
Suspension, using the partial size of syringe type filter adjustment liposome suspension to 100~150nm to get rouge containing growth factor
Plastid.
It is formulated preparation step reference formulation 1 of the 2-5 containing researching advances on growth factor liposomes.
The preparation of 4 pox-dispelling function whitening skin-protection cream of embodiment
The preparation of 4 pox-dispelling function whitening skin-protection cream of embodiment includes the component of weight percent meter shown in the following table 2:
The formula composition of 2 pox-dispelling function whitening skin-protection cream of table
Preparation step:
(1) by the deionized water of formula ratio, glycerol, undecylenoyl phenylalanine, plants fruits and seeds superfine powder and propylene
Sour sodium/sodium acryloyldimethyl taurate copolymers are added water phase pot, are heated to 75 DEG C, stir evenly, obtain water phase；
(2) tocopherol of formula ratio, phytosterol/octyl dodecanol lauroyl glutamate ester, hydrogenated coco, glycerol is hard
Resin acid ester, cholesterine polyethers -24, docosyl alcohol, stearyl alcohol glycyrrhetin acid esters and blue gum leaf oil are added oily phase pot, are heated to 75 DEG C,
It stirs evenly, obtains oily phase；
(3) water phase is pumped into vacuum emulsifying machine, opens stirring and vacuum, be -0.04MPa, stir in 80 DEG C, vacuum degree
Under conditions of revolving speed is 80r/min, by oily mutually sucking mulser, mixed liquor is obtained, then the homogeneous at 2000r/min
15min；
(4) it after the completion of homogeneous, keeps vacuum degree and mixing speed constant, is cooled to 40 DEG C, Radix Aristolochiae extract, angle is added
Fruit tree extract, contains researching advances on growth factor liposomes, Phenoxyethanol and Sensiva SC50 at Rhizoma Anemones Altaicae extract, and it is equal to continue stirring
It is even, it is cooled to room temperature, discharges, it is filling to get pox-dispelling function whitening skin-protection cream.
The preparation of 1 pox-dispelling function whitening skin-protection cream of comparative example
The preparation of 1 pox-dispelling function whitening skin-protection cream of this comparative example includes the component of weight percent meter shown in the following table 3:
The formula composition of 3 pox-dispelling function whitening skin-protection cream of table
Preparation step reference implementation example 4.
Test example one, human skin patch
The related request of " human skin patch " in 2015 editions " cosmetics health specifications " issued referring to the Ministry of Public Health
1-5 is formulated to the embodiment of the present invention 4 with measuring method, comparative example 1 is formulated pox-dispelling function whitening skin-protection cream made from 6-11 and carries out
Test, respectively 0h, 6h, 12h, for 24 hours, 48h observe dermoreaction, the experimental result of all trial volunteers is negative knot
Fruit does not occur skin adverse reaction.Show that pox-dispelling function whitening skin-protection cream provided by the invention is nonirritant, it is highly-safe.
Test example two, bacteriostatic test
It prepares propionibacterium acnes, staphylococcus aureus, staphylococcus epidermis plate respectively using double-layer agar technique, puts down
Plate bacteria containing amount is 104~105CFU/ plate accurately weighs the formula of 0.2g embodiment 4 1-5, comparative example 1 is formulated made from 6-11 and dispels
Acne is uniformly applied on plate containing bacterium except print whitening skin-protection cream with aseptic cotton carrier, and culture 24 is small under anaerobic condition at 36 ± 1 DEG C
When, bacterium colony growth in plate is then observed, the results are shown in Table 4.
4 each group fungistatic effect of table compares
The results show that embodiment 4, which is formulated pox-dispelling function whitening skin-protection cream made from 1-5, is showed no propionibacterium acnes, gold
Staphylococcus aureus and the growth of staphylococcus epidermis bacterium colony, show sample to propionibacterium acnes, staphylococcus aureus and table
The staphylococcic inhibitory effect of skin is stronger, and sample anti-acne effect is good.
6-8 is formulated by comparative example 1 it is found that the liposome in formula is free of the filter of Anji white tea callus tissue culture respectively
Liquid or simultaneously be free of Anji white tea callus tissue culture filtrate and Vitexin, or formula in directly do not add liposome, make
The fungistatic effect of face cream reduces, and shows Crithmum maritimum callus tissue culture filtrate, Vitexin to the antibacterial effect for improving face cream
Fruit tool has certain effect.By the formula of comparative example 19 it is found that being free of Radix Aristolochiae extract in formula, propionibacterium acnes bacterium colony is complete
Full growth, although showing that the other compositions in formula have certain fungistatic effect, to propionibacterium acnes without effect.By comparing
It is found that being free of ceriops tagal extract in formula, fungistatic effect is substantially reduced the formula of example 1 10.By the formula 11 of comparative example 1 it is found that matching
Plants fruits and seeds superfine powder is free of in side, does not influence fungistatic effect.The above result shows that pox-dispelling function whitening skin-protection cream of the present invention is each
Ingredient is mutually coordinated, plays splendid fungistatic effect.
Test example three, oil-control test
Screening male trial volunteer 110, the age 18-30 years old, all trial volunteers were oily skin, and are suffered from
Acne meets subject enrollment standard, can cooperate experiment very well, regularity in one's life is able to maintain during research.Will aspiration by
Examination person is divided into 11 groups, every group of 10 people, respectively test 1-5 group and comparison 1-6 group, wherein test 1-5 group is matched using embodiment 4
Pox-dispelling function whitening skin-protection cream made from square 1-5, comparison 1-6 group are formulated pox-dispelling function whitening made from 6-11 using comparative example 1
Face cream.
Test method: cheek, temple and corners of the mouth intersection at experiment septum reset or so cheekbone, nose and pupil intersection or so
Locate left and right sides as test area.Trial volunteer is early daily, evening adheres to using corresponding tested products.Experiment starts rear 15
It, progress follow-up in 30 days, test subject is changed using the skin moisture content after product, fat content respectively, the same will
The test of hope person is completed by the same survey crew.In the 0th day, 15 days, progress clinical test results assessment in 30 days.
Wherein, the measurement of skin oil and fat content: the skin oil and fat produced using Courage+Khazaka (CK) company of Germany
Content measuring instrument Sebumeter is surveyed 5 times and is averaged.The measurement of skin moisture content: Germany Courage+Khazaka is used
(CK) the moisture of skin tester Corneometer CM825 of company's production, which is surveyed 5 times, is averaged.Test result is referring to table 5.
The skin oil and fat of 5 each group trial volunteer of table, moisture content test result
The results show that the embodiment of the present invention 4 be formulated 1-5 made from pox-dispelling function whitening skin-protection cream can obviously improve aspiration by
The water and oil balance of Shi Zhe face, effect are substantially better than the use that comparative example 1 is formulated pox-dispelling function whitening skin-protection cream made from 6-11
Effect.Show that pox-dispelling function whitening skin-protection cream of the present invention can change the microcirculqtory system of skin, adjusts skin water and oil balance, subtract
Generation that is light and eliminating acne.
Test example four, anti-acne effect evaluation
Screening trial volunteer 264, wherein male 154, women 110, the age 18-35 years old, all aspirations were tested
There are apparent acne and small pox in person face, and no skin allergy history meets subject enrollment standard, can cooperate experiment very well,
Regularity in one's life is able to maintain during research.Trial volunteer is divided into 11 groups, every group of 24 people, wherein 14 people of male, women 10
People, respectively test 1-5 group and comparison 1-6 group, wherein test 1-5 group is formulated pox-dispelling function made from 1-5 using embodiment 4
Whitening skin-protection cream, comparison 1-6 group are formulated pox-dispelling function whitening skin-protection cream made from 6-11 using comparative example 1.
Test method: taking appropriate given the test agent to be coated in clean affected part, 2 times a day, each 1 time sooner or later, uses 28 days.Will
It is willing to that subject keeps good daily life system and eating habit in the trial employment period.The feedback of subject is counted after 28 days.Its
In, effectively: acne and small pox are reduced, and the senses of discomfort such as red swelling of the skin, inflammation mitigate, acne print desalination or recession.It the results are shown in Table 6.
6 each group anti-acne of table eliminates acne print evaluation result
The results show that the embodiment of the present invention 4 is formulated the anti-acne and removal acne print of pox-dispelling function whitening skin-protection cream made from 1-5
Significant effect, anti-acne it is efficient up to 79.2~87.5%, remove the effective percentage of acne print up to 75~83.3%, can obviously reduce
Acne and small pox, mitigate the senses of discomfort such as red swelling of the skin, inflammation, desalination or recession acne print, and effect is formulated 6-11 system better than comparative example 1
The pox-dispelling function whitening skin-protection cream obtained.
The above is only the preferred embodiment of the present invention, it is noted that above-mentioned preferred embodiment is not construed as pair
Limitation of the invention, protection scope of the present invention should be defined by the scope defined by the claims..For the art
For those of ordinary skill, without departing from the spirit and scope of the present invention, several improvements and modifications can also be made, these change
It also should be regarded as protection scope of the present invention into retouching.
Claims (8)
1. a kind of pox-dispelling function whitening skin-protection cream, which is characterized in that by mass percentage, including following components: glycerol 2~
6%, tocopherol 1~3%, phytosterol/octyl dodecanol lauroyl glutamate ester 0.5~1.5%, hydrogenated coco 1~2%,
Stearine 1~2%, cholesterine polyethers -24 1~3%, docosyl alcohol 0.5~2%, stearyl alcohol glycyrrhetin acid esters 1~
2%, blue gum leaf oil 0.5~1.5%, undecylenoyl phenylalanine 1~3%, Radix Aristolochiae extract 1~4%, ceriops tagal mention
Take object 1~3%, Rhizoma Anemones Altaicae extract 1~4%, containing researching advances on growth factor liposomes 8~12%, plants fruits and seeds superfine powder 6~8%,
Sodium acrylate/sodium acryloyldimethyl taurate copolymers 0.5~1.5%, Phenoxyethanol 0.05~0.1%, ethylhexyl are sweet
Oil 0.05~0.1%, surplus is deionized water.
2. pox-dispelling function whitening skin-protection cream according to claim 1, which is characterized in that the lipid containing growth factor
Body, according to parts by weight, include following components: 10~20 parts of Anji white tea callus tissue culture filtrate, recombined human cutin are thin
0.00005~0.001 part of the intracellular growth factor, 1~3 part of Vitexin, 35~45 parts of hydrogenated soy phosphatidyl choline, 5~15 parts of cholesterol,
5~15 parts and 4~7 parts of Tween-80 of cupreol.
3. pox-dispelling function whitening skin-protection cream according to claim 2, which is characterized in that the lipid containing growth factor
Body includes according to parts by weight following components: 15 parts of Anji white tea callus tissue culture filtrate, recombination human body keratinized cell are raw
0.0001 part of the long factor, 2 parts of Vitexin, 40 parts of hydrogenated soy phosphatidyl choline, 10 parts of cholesterol, 5 parts of cupreol and Tween-80 5
Part.
4. pox-dispelling function whitening skin-protection cream according to claim 2, which is characterized in that described contains researching advances on growth factor liposomes
Preparation the following steps are included:
(1) Vitexin, hydrogenated soy phosphatidyl choline, cholesterol, cupreol and the Tween-80 of formula ratio are weighed, is mixed, nothing is added
Water-ethanol is sufficiently stirred dissolution, obtains solution A；
(2) recombinant human horny cell growth factor-2 is dissolved in the PBS buffer solution that pH is 7.2, stirs to being completely dissolved, is made
Then the Anji white tea callus tissue culture filtrate of formula ratio is added in the solution of 0.1~0.2mg/mL, be uniformly mixed, obtain
Solution B；
(3) in the case where magnetic stirring apparatus is stirred continuously, solution B is injected into solution A with syringe, is sufficiently mixed 0.5~1h, so
Afterwards at 28~32 DEG C, rotary evaporation under conditions of 0.1~1MPa removes ethyl alcohol, then oscillation aquation is anti-in 30~35 DEG C of water-baths
1~2h is answered, liposome suspension is obtained, using the partial size of syringe type filter adjustment liposome suspension to 100~150nm,
Up to containing researching advances on growth factor liposomes.
5. pox-dispelling function whitening skin-protection cream according to claim 1, which is characterized in that the plants fruits and seeds superfine powder is
Moringa seeds, Semen Vitis Amurensis and buckwheat seed are with (1~2): (0.5~1): the mass ratio of (1~3) forms.
6. pox-dispelling function whitening skin-protection cream according to claim 5, which is characterized in that the plants fruits and seeds superfine powder is
Moringa seeds, Semen Vitis Amurensis and buckwheat seed are formed with the mass ratio of 1.5:1:2.5.
7. pox-dispelling function whitening skin-protection cream according to claim 5, which is characterized in that the plants fruits and seeds superfine powder
Preparation mixes the following steps are included: by the moringa seeds of formula ratio, Semen Vitis Amurensis and buckwheat seed, crushes, obtains seed mixed powder
End；Seed mixed-powder is added in airflow milling powder machine, carries out airflow milling, the micro mist sufficiently ground is with air-flow by dividing
The gap of wheel is selected, and what is do not ground sufficiently cannot then pass through, and continue airflow milling, until the partial size of powder is 0.2~1 μ
M, collection obtain plants fruits and seeds superfine powder.
8. a kind of method for preparing pox-dispelling function whitening skin-protection cream described in claim 1, which is characterized in that including following step
It is rapid:
(1) by the deionized water of formula ratio, glycerol, undecylenoyl phenylalanine, plants fruits and seeds superfine powder and sodium acrylate/
Sodium acryloyldimethyl taurate copolymers are added water phase pot, are heated to 70~75 DEG C, stir evenly, obtain water phase；
(2) by the tocopherol of formula ratio, phytosterol/octyl dodecanol lauroyl glutamate ester, hydrogenated coco, glycerol stearate
Ester, cholesterine polyethers -24, docosyl alcohol, stearyl alcohol glycyrrhetin acid esters and blue gum leaf oil are added oily phase pot, are heated to 70~75 DEG C,
It stirs evenly, obtains oily phase；
(3) will water phase be pumped into vacuum emulsifying machine in, open stirring and vacuum, 80~85 DEG C, vacuum degree be -0.06~-
Under conditions of 0.04MPa, speed of agitator are 60~80r/min, by oily mutually sucking mulser, mixed liquor are obtained, is then existed
10~15min of homogeneous under 2000~3000r/min；
(4) it after the completion of homogeneous, keeps vacuum degree and mixing speed constant, is cooled to 40~45 DEG C, Radix Aristolochiae extract, angle is added
Fruit tree extract, contains researching advances on growth factor liposomes, Phenoxyethanol and Sensiva SC50 at Rhizoma Anemones Altaicae extract, and it is equal to continue stirring
It is even, it is cooled to room temperature, discharges, it is filling to get pox-dispelling function whitening skin-protection cream.
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| CN201811174875.8A CN109157470A (en) | 2018-10-09 | 2018-10-09 | A kind of pox-dispelling function whitening skin-protection cream and preparation method thereof |
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| CN201811174875.8A CN109157470A (en) | 2018-10-09 | 2018-10-09 | A kind of pox-dispelling function whitening skin-protection cream and preparation method thereof |
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109771313A (en) * | 2019-04-02 | 2019-05-21 | 靖西市秀美边城农业科技有限公司 | A kind of anti-acne jelly and preparation method thereof |
| CN115531229A (en) * | 2022-11-08 | 2022-12-30 | 浙江海心智惠科技有限公司 | Preparation method of skin-moistening plant essential oil |
| CN117045563A (en) * | 2023-09-28 | 2023-11-14 | 云南英格生物技术有限公司 | Radix aucklandiae root extract and its preparation method and application |
-
2018
- 2018-10-09 CN CN201811174875.8A patent/CN109157470A/en not_active Withdrawn
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109771313A (en) * | 2019-04-02 | 2019-05-21 | 靖西市秀美边城农业科技有限公司 | A kind of anti-acne jelly and preparation method thereof |
| CN115531229A (en) * | 2022-11-08 | 2022-12-30 | 浙江海心智惠科技有限公司 | Preparation method of skin-moistening plant essential oil |
| CN117045563A (en) * | 2023-09-28 | 2023-11-14 | 云南英格生物技术有限公司 | Radix aucklandiae root extract and its preparation method and application |
| CN117045563B (en) * | 2023-09-28 | 2023-12-29 | 云南英格生物技术有限公司 | Radix aucklandiae root extract and its preparation method and application |
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Application publication date: 20190108 |