CN109111530A - 一种具有抗氧化活性的复合多糖及其制备方法和应用 - Google Patents
一种具有抗氧化活性的复合多糖及其制备方法和应用 Download PDFInfo
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Abstract
本发明公开了一种具有抗氧化活性的复合多糖及其制备方法和应用。所述复合多糖是由葡萄糖、甘露糖、***糖、半乳糖、木糖、半乳糖醛酸、葡萄糖醛酸组成,摩尔质量比为50.6~51.8:20.4~20.9:2.9~3.1:2.6~2.8:1.7~2.0:17.2~17.8:1.8~4.2。制备方法是将40~120份山楂、20~60份荷叶、20~60份苦荞麦、15~45份决明子、10~30份枸杞、10~30份茯苓分别烘干粉碎,再混合均匀后,依次经过水提、抽滤、浓缩、醇沉、离心、透析、冷冻干燥,得到复合多糖。本发明制备的复合多糖纯度高、抗氧化活性强,提取工艺简便、成本低廉、可操作性强,可广泛应用于药物、保健食品等行业中,同时也可作为中间体广泛运用于抗氧化各个领域中。
Description
技术领域
本发明属于药物及保健品领域,具体涉及一种复合多糖及其制备方法和应用,尤其是一种具有抗氧化活性的复合多糖及其制备方法和应用。
背景技术
当人体在与外界环境接触时,阳光辐射、空气污染、农药、药物、放射、吸烟、压力等因素都会使人体产生自由基,过量自由基通过多种途径诱导人体组织、器官的细胞衰老和凋亡,引起DNA损伤,抑制细胞周期进程;通过影响体内酶代谢,加速脂肪和蛋白质的分解,促进糖异生过程,削弱糖氧化功能作用;降低机体抵抗力和免疫力,引发衰老、肿瘤、高血压、心脏病、糖尿病等疾病。
科学研究表明,多糖具有清除自由基的抗氧化作用,通过捕捉脂质过氧化链式反应中产生的自由基,从而减少脂质过氧化反应链长度、阻断或减缓脂质过氧化的进行,达到对自由基的直接清除作用;通过与产生自由基所必需的金属离子发生络合作用,对自由基起间接清除作用;多糖可以提高SOD、CAT、GSH-Px等抗氧化酶的活性,从而发挥抗氧化作用。
发明内容
本发明的目的在于提供一种纯度高、提取工艺简便、抗氧化活性强的复合多糖。
本发明的另一目的在于提供上述具有抗氧化活性的复合多糖的制备方法。
本发明的再一目的在于提供上述具有抗氧化活性的复合多糖的实际应用。
为实现本发明的目的,本发明采用如下技术方案:
一种具有抗氧化活性的复合多糖,
由葡萄糖、甘露糖、***糖、半乳糖、木糖、半乳糖醛酸、葡萄糖醛酸按照摩尔质量比50.6~51.8:20.4~20.9:2.9~3.1:2.6~2.8:1.7~2.0:17.2~17.8:1.8~4.2组成。
一种具有抗氧化活性的复合多糖的制备方法,将40~120份山楂、20~60份荷叶、20~60份苦荞麦、15~45份决明子、10~30份枸杞、10~30份茯苓分别烘干粉碎,再混合均匀后,依次经过水提、抽滤、浓缩、醇沉、离心、透析、冷冻干燥,得到复合多糖。
而且,山楂、荷叶、苦荞麦、决明子、枸杞、茯苓的重量比为4:2:2:1.5:1:1。
而且,所述水提是加水体积是原料量的5~40倍v/m,水温40~80℃,提取次数1~4,提取时间15~120min。
而且,所述浓缩为浓缩温度50~60℃,浓缩至浓缩前体积的1/5。
而且,所述醇沉是指加入无水乙醇至乙醇浓度为75~80%v/v进行沉淀。
而且,所述透析为用Mw3500的透析袋自来水透析2天,蒸馏水透析1天。
本发明中所述山楂、荷叶、苦荞麦、决明子、枸杞、茯苓是本领域技术人员公知的,可以通过商业途径购买得到,可以为药材,也可以是饮片,只要其符合《中华人民共和国药典》(2015年版)质量标准即可。
本发明所述复合多糖的使用剂量和使用方法取决于诸多因素,包括使用者的年龄、体重、性别、自然健康状况、营养状况、使用时间、代谢速率、病程严重程度以及主治医师的主观判断。本领域的技术人员依据上述因素可以确定所述复合多糖的使用剂量和使用方法。
本领域技术人员可以根据不同使用者的需求将本发明所述复合多糖加入制备不同剂型时所需的各种常规的添加剂,如崩解剂、润滑剂、乳化剂、粘合剂等,以常规制剂方法,制成常用药物或保健食品,如胶囊剂、片剂、丸剂、口服液、粉剂、颗粒剂、散剂等。
本发明提供了一种具有抗氧化作用的保健食品,包括有效量的本发明所述复合多糖和保健食品中可接受的辅料。
所述保健食品优选为胶囊剂、片剂、丸剂、粉剂、颗粒剂、散剂或口服液。
本发明还提供了一种具有抗氧化作用的药物,包括有效量的本发明所述复合多糖和药学上可接受的辅料。
所述药物优选为胶囊剂、片剂、丸剂、粉剂、颗粒剂、散剂或口服液。
所述一种具有抗氧化活性的复合多糖在抗氧化活性领域的应用,特别用于抗氧化活性保健食品和药物领域。
本发明的优点和有益效果:
1、本发明制备的复合多糖纯度高、抗氧化活性强,对DPPH、ABTS、OH自由基清除率分别达到93.56%、83.29%、84.03%。
2、本发明提取工艺简便、成本低廉、可操作性强,可广泛应用于药物、保健食品等行业中,同时也可作为中间体广泛运用于抗氧化各个领域中。
附图说明
图1为本发明所述复合多糖的傅里叶红外光谱图;
图2为复合多糖对DPPH自由基清除率效果图;
图3为复合多糖对ABTS自由基清除率效果图;
图4为复合多糖对OH自由基清除率效果图。
具体实施方式
本发明公开了一种具有抗氧化活性的复合多糖及其制备方法和应用。本领域技术人员可以借鉴本文内容,适当改进工艺参数实现。特别需要指出的是,所有类似的替换和改动对本领域技术人员来说是显而易见的,它们都被视为包括在本发明。本发明的方法及产品已经通过较佳实施例进行了描述,相关人员明显能在不脱离本发明内容、精神和范围内对本文所述的方法进行改动或适当变更与组合,来实现和应用本发明技术。
为了进一步理解本发明,下面将结合本发明实施例,对本发明实施例中的技术方案进行清楚、完整地描述,显然,所描述的实施例仅仅是本发明一部分实施例,而不是全部的实施例。基于本发明中的实施例,本领域普通技术人员在没有做出创造性劳动前提下所获得的所有其他实施例,都属于本发明保护的范围。
实施例1
一种具有抗氧化活性的复合多糖,由葡萄糖、甘露糖、***糖、半乳糖、木糖、半乳糖醛酸、葡萄糖醛酸按照摩尔质量比50.6:20.4:3.1:2.6:1.7组成。
制备方法为:将山楂40份、荷叶20份、苦荞麦20份、决明子15份、枸杞10份、茯苓10份分别于40~60℃烘干至恒重、粉碎。然后进行混合,加入2875mL水量,提取温度55℃,提取时间45min,提取次数3,接着抽滤,收集滤液加入0.2%果胶酶于50℃处理2h,随后于95℃灭活5min,经55℃浓缩至浓缩前体积的1/5,其后加入无水乙醇至乙醇浓度为80%(v/v)进行沉淀,于4500r/min离心15min,收集上清液并用Mw3500的透析袋自来水透析2天,蒸馏水透析1天,将透析液于-20℃冷冻干燥,最后得到复合多糖,多糖得率为5.47%。
实施例2
将山楂40份、荷叶20份、苦荞麦20份、决明子15份、枸杞10份、茯苓10份分别于40~60℃烘干至恒重、粉碎。然后进行混合,然后加入3450mL水量,提取温度60℃,提取时间75min,提取次数3,接着抽滤,收集滤液加入0.2%果胶酶于50℃处理2h,随后于95℃灭活5min,经55℃浓缩至浓缩前体积的1/5,其后加入无水乙醇至乙醇浓度为80%(v/v)进行沉淀,于4500r/min离心15min,收集上清液并用Mw3500的透析袋自来水透析2天,蒸馏水透析1天,将透析液于-20℃冷冻干燥,最后得到复合多糖,多糖得率为5.85%。
实施例3
将山楂40份、荷叶20份、苦荞麦20份、决明子15份、枸杞10份、茯苓10份分别于40~60℃烘干至恒重、粉碎。然后加入4025mL水量,提取温度65℃,提取时间60min,提取次数3,接着抽滤,收集滤液加入0.2%果胶酶于50℃处理2h,随后于95℃灭活5min,经55℃浓缩至浓缩前体积的1/5,其后加入无水乙醇至乙醇浓度为80%(v/v)进行沉淀,于4500r/min离心15min,收集上清液并用Mw3500的透析袋自来水透析2天,蒸馏水透析1天,将透析液于-20℃冷冻干燥,最后得到复合多糖,多糖得率为6.35%。
对本发明的复合多糖进行傅里叶红外光谱分析,如图1所示,所述复合多糖具备以下主要官能团:O-H,3388.93cm-1;C-H,2975.27,2928.88,2898.95cm-1;C=O,1716.34,1609.28,1416.72cm-1;C-O-C,1146.65,1088.71cm-1;C-O-H,1055.02cm-1。
对本发明的复合多糖对DPPH、ABTS、OH自由基清除率进行分析,如图2~4所示,结果表明,本发明制备的复合多糖对DPPH、ABTS、OH自由基清除率分别达到93.56%、83.29%、84.03%,具有很强的抗氧化活性。
Claims (8)
1.一种具有抗氧化活性的复合多糖,其特征在于:
由葡萄糖、甘露糖、***糖、半乳糖、木糖、半乳糖醛酸、葡萄糖醛酸按照摩尔质量比50.6~51.8:20.4~20.9:2.9~3.1:2.6~2.8:1.7~2.0:17.2~17.8:1.8~4.2组成。
2.根据权利要求1所述的具有抗氧化活性的复合多糖的制备方法,其特征在于:将40~120份山楂、20~60份荷叶、20~60份苦荞麦、15~45份决明子、10~30份枸杞、10~30份茯苓分别烘干粉碎,再混合均匀后,依次经过水提、抽滤、浓缩、醇沉、离心、透析、冷冻干燥,得到复合多糖。
3.根据权利要求2所述的具有抗氧化活性的复合多糖的制备方法,其特征在于:山楂、荷叶、苦荞麦、决明子、枸杞、茯苓的重量比为4:2:2:1.5:1:1。
4.根据权利要求2所述的具有抗氧化活性的复合多糖的制备方法,其特征在于:所述水提是加水体积是原料量的5~40倍v/m,水温40~80℃,提取次数1~4,提取时间15~120min。
5.根据权利要求2所述的具有抗氧化活性的复合多糖的制备方法,其特征在于:所述浓缩为浓缩温度50~60℃,浓缩至浓缩前体积的1/5。
6.根据权利要求2所述的具有抗氧化活性的复合多糖的制备方法,其特征在于:所述醇沉是指加入无水乙醇至乙醇浓度为75~80%v/v进行沉淀。
7.根据权利要求2所述的具有抗氧化活性的复合多糖的制备方法,其特征在于:所述透析为用Mw3500的透析袋自来水透析2天,蒸馏水透析1天。
8.一种权利要求1~7任一权利要求所述的复合多糖在制备具有抗氧化作用的药物或保健食品中的应用。
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