CN109105702A - A kind of preparation method of composition containing NADH, application and energy extender - Google Patents
A kind of preparation method of composition containing NADH, application and energy extender Download PDFInfo
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- CN109105702A CN109105702A CN201811001002.7A CN201811001002A CN109105702A CN 109105702 A CN109105702 A CN 109105702A CN 201811001002 A CN201811001002 A CN 201811001002A CN 109105702 A CN109105702 A CN 109105702A
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- CDAISMWEOUEBRE-UHFFFAOYSA-N scyllo-inosotol Natural products OC1C(O)C(O)C(O)C(O)C1O CDAISMWEOUEBRE-UHFFFAOYSA-N 0.000 description 1
- 230000035945 sensitivity Effects 0.000 description 1
- WXMKPNITSTVMEF-UHFFFAOYSA-M sodium benzoate Chemical compound [Na+].[O-]C(=O)C1=CC=CC=C1 WXMKPNITSTVMEF-UHFFFAOYSA-M 0.000 description 1
- 235000010234 sodium benzoate Nutrition 0.000 description 1
- 239000004299 sodium benzoate Substances 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
- 239000005720 sucrose Substances 0.000 description 1
- 230000002195 synergetic effect Effects 0.000 description 1
- 238000009210 therapy by ultrasound Methods 0.000 description 1
- 210000001541 thymus gland Anatomy 0.000 description 1
- 230000001256 tonic effect Effects 0.000 description 1
- 238000012795 verification Methods 0.000 description 1
- 210000001835 viscera Anatomy 0.000 description 1
- 235000019158 vitamin B6 Nutrition 0.000 description 1
- 239000011726 vitamin B6 Substances 0.000 description 1
- 229940011671 vitamin b6 Drugs 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L2/00—Non-alcoholic beverages; Dry compositions or concentrates therefor; Their preparation
- A23L2/38—Other non-alcoholic beverages
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
- A23L33/10—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2002/00—Food compositions, function of food ingredients or processes for food or foodstuffs
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- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Nutrition Science (AREA)
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Food Science & Technology (AREA)
- Polymers & Plastics (AREA)
- Mycology (AREA)
- Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
Abstract
The invention discloses the preparation methods of a kind of composition containing NADH, application and its energy extender, are related to pharmaceutical technology field.Composition disclosed by the invention containing NADH, in parts by weight, 1-10 parts of NADH, 1-10 parts of Co-Q10s, 1-10 parts of chondroitin sulfates, 1-10 parts of ursodesoxycholic acid, 1-10 parts of glycosyls isoflavones, 1-10 parts of s- Ademetionines, 1-10 parts of γ-aminobutyric acids, 1-10 parts of PPD and 1-10 parts of Rh2.The composition can be used for preparing alleviation and/or reduce the beverage of fatigue.Energy extender of the technical solution of the present invention rich in NADH composition not only meets adaptation movement or the physilogical characteristics of physical exertion crowd in nutritional ingredient and content, it can also be by rapid osmotic absorption, achieve the effect that keep the skin wet, electrolyte and energy, makes to mobilize and quickly regain one's strength.The preparation process of energy extender is simple, mild condition, is suitable for industrialized mass production.
Description
Technical field
The invention belongs to pharmaceutical technology field more particularly to a kind of compositions containing NADH, application and energy extender
Preparation method.
Background technique
On the run, if tired driver, it may appear that following situation: (1) velocity perturbation, such as in maximum speed
It is travelled on 100 kilometers/hour of road, it is desirable that driver only controls running speed at 95 kilometers/hour with the mode stepped on the gas.If
Driver is tired, and the speed of car can fluctuate above and below fixing speed, and driver is more tired, and running speed fluctuation is more severe；(2) side is moved
Position cannot be maintained at road center traveling or left or right, the more tired driving deviation of driver are more severe when driver has been stranded.With
Upper two kinds of fatigue driving situations are breakneck.In general, driver is sleepy and tired in order to be resisted by drinking functional beverage.
The main adding ingredient of energy of in energy drink, can refreshing oneself, supplement is artificial caffeine, taurine, VB race etc..But
It is the habituation that the energy drink containing artificial caffeine is easy to cause body to caffeine, stimulates gastric mucosa, increases gastric acid point
It secretes, is not able to satisfy healthier, more natural pursuit demand of the people to food, beverage etc..
Energy drink is for the beverage of supplying human body energy, vitamin contained therein (generally B family vitamin) and carbon
Hydrate (mainly white granulated sugar, sucrose) is converted into energy under interaction, regains one's strength of body, relieves fatigue to drinking person.
The beverage of many supplement body energies currently on the market, most of is all the energy drink containing hormone, although can temporarily mend
Energy is filled, but has side effect, seriously affects the health of human body.
Current market sales of red ox anti-fatigue beverage, is by caffeine, taurine, lysine, inositol, vitamin
B6, vitamin B12, citric acid, sodium benzoate, lemon yellow, famille rose, white granulated sugar and water composition.There is certain antifatigue function
Effect, but be not especially effectively for reducing the feeling of fatigue effect for driving to generate for a long time.Therefore, it is necessary to targetedly develop
A kind of antifatigue functional beverage to realize the health of safe driving target of energy.
Summary of the invention
Present invention aims at the shortcomings for solving existing composition, provide a kind of antifatigue, safe and healthy energy
Replenishers.
To solve the above-mentioned problems, the present invention provides the application and energy extender of a kind of composition containing NADH
Preparation method.
Concrete scheme is as follows:
In a first aspect, the present invention proposes that a kind of composition containing NADH, the composition include following parts by weight of component:
1-10 parts of NADH, 1-10 parts of Co-Q10s, 1-10 parts of chondroitin sulfates, 1-10 parts of ursodesoxycholic acid, the soybean of 1-10 parts of glycosyls are different
Flavones, 1-10 parts of s- Ademetionines, 1-10 parts of γ-aminobutyric acids, 1-10 parts of PPD and 1-10 parts of Rh2.
Its further technical solution are as follows: further include auxiliary material, the auxiliary material includes at least one of following parts by weight of component:
0.01-0.05 parts of sodium chloride, 0.01-0.05 parts of potassium chloride, 0.001-0.005 parts of vitamin B12s, 0.001-0.01 parts of vitamins
C, 0.001-0.005 parts of vitamin B6s, 0.02-0.05 parts of taurines, 3-6 parts of honey, 0.005-0.01 parts of magnesium chlorides.
Its further technical solution are as follows: further include auxiliary material, the auxiliary material includes at least five kinds of following parts by weight of component:
0.01-0.05 parts of sodium chloride, 0.01-0.05 parts of potassium chloride, 0.001-0.005 parts of vitamin B12s, 0.001-0.01 parts of vitamins
C, 0.001-0.005 parts of vitamin B6s, 0.02-0.05 parts of taurines, 3-6 parts of honey, 0.005-0.01 parts of magnesium chlorides.
The invention also provides the energy extenders described in any of the above-described technical solution containing NADH to prepare in preparation
Alleviate and/or reduce the application in tired beverage.
Second aspect, the present invention propose a kind of energy extender of composition containing NADH, including such as first aspect institute
The composition stated.
The present invention also proposes a kind of side of energy extender for preparing the composition containing NADH as described in second aspect
Method includes the following steps:
S1, by NADH, Co-Q10, chondroitin sulfate, ursodesoxycholic acid, the isoflavones of glycosyl, s- adenosine egg ammonia
Acid, γ-aminobutyric acid, PPD and Rh2 are separately dried, crush, crossing 30-80 mesh, and weighing mixing in proportion；
S2, the raw material into step S1 are added water and stir and evenly mix, and then heat to 50-60 DEG C of extraction 1-3h, are mixed
Object；
S3, the mixture in step S2 is filtered, obtains leaching liquor；
S4, the leaching liquor that step S3 is added in boiling water and auxiliary material are uniformly mixed, and 50-60 DEG C are maintained the temperature at, at homogeneous
Reason, degassing sterilize, are canned, obtain the energy extender containing NADH.
Its further technical solution are as follows: the mass ratio of raw material and water in the step S2 is 1:5-10.
Its further technical solution are as follows: the volume ratio of water is added in leaching liquor obtained in the step S3 and step S2
For 1/5-3/5.
Its further technical solution are as follows: after the auxiliary material in the step S4 is dissolved completely with water, add in boiling water, institute
The mass ratio for stating auxiliary material and water is 1:0.5-5.
Its further technical solution are as follows: the leaching liquor in the step S4: the volume ratio of boiling water is 1:5-40.
Compared with prior art, the attainable technical effect of present invention institute includes:
Energy extender provided by the invention rich in NADH composition, which not only meets in nutritional ingredient and content, adapts to fortune
Dynamic or physical exertion crowd physilogical characteristics, moreover it is possible to by rapid osmotic absorption, reach keep the skin wet, the effect of electrolyte and energy
Fruit makes mobilization quickly regain one's strength.The present invention can appropriate adjustment sodium chloride, potassium chloride as needed additive amount, make finally to produce
The stability of product is guaranteed.
In addition, the present invention is specifically matched using specific ingredient, reasonable recipe, compatibility is proper, ingredient selected by the present invention
Compatibility is suitable, meets the theory that traditional Chinese medicine and modern medicine combine, easy to use, good absorption effect, no bad and malicious secondary work
With according to clinical verification, capable of effectively improving the energy supply of human body in a short time, have in sports drink field and very big push away
Wide and application prospect.
The preparation method of the preparation of energy extender of the present invention containing NADH, step is simple, mild condition, fits
It is suitable for industrialized mass production.
Specific embodiment
Be specifically described the contents of the present invention with experiment by the following examples, these embodiments and experiment be intended merely into
One step illustrates the contents of the present invention and illustrates that interest field of the invention by these embodiments and does not test described content
Limitation.Based on the embodiments of the present invention, those of ordinary skill in the art are obtained without creative efforts
Every other embodiment, shall fall within the protection scope of the present invention.
Embodiment 1
The present embodiment provides a kind of compositions containing NADH, and in parts by weight, the composition includes 1 part of NADH, 3 parts
Co-Q10,7 parts of chondroitin sulfates, 5 parts of ursodesoxycholic acid, 10 parts of glycosyls isoflavones, 3 parts of s- Ademetionines, 1 part
γ-aminobutyric acid, 6 parts of PPD and 2 part of Rh2.
Composition provided in an embodiment of the present invention containing NADH, wherein NADH (i.e. nicotinamide adenine dinucleotide)
Preparation method include:
Extract the crude extract or its pure enzyme of nicotinamide-nucleotide adenylyltransferase；
The crude extract or its pure enzyme of immobilization recombination nicotinamide-nucleotide adenylyltransferase；
It is catalyzed with immobilization recombination nicotinamide-nucleotide adenylyltransferase, with nicotinamide nucleotide and trinosin
(ATP) nicotinamide adenine dinucleotide is prepared for substrate.
Specific steps are as follows:
By the plasmid pRSET-bmj transformed competence colibacillus bacterial cell of niacinamide-containing nucleosides adenylyl transferase gene
E.coliHB101 is cultivated 24 hours at upper 37 DEG C of Luriabroth (LB) plate (kanamycins containing 100mg/L).Inoculation is single
It is cloned in 5 milliliters of LB liquid mediums (kanamycins containing 100mg/L) and is cultivated 2024 hours in 30 DEG C.Thalline were collected by centrifugation,
And it is suspended in 1 milliliter of 100mM Tris hydrochloride buffer (pH7.5).Then ultrasonic treatment bacterial cell is used.Centrifugation (10
DEG C, 17,800g, 10 minutes) and collect supernatant, as thick leach protein (or crude extract).The nicotinamide riboside adenosine of recombination
Acyltransferase thick leach protein is heat-treated 10 minutes through 70 DEG C, is centrifuged (10 DEG C, 17,800g, 10 minutes) and is collected supernatant, as
Partially purified albumen.
Nicotinamide-nucleotide adenylyltransferase immobilization
Nicotinamide-nucleotide adenylyltransferase thick leach protein or partially purified albumen are taken, with washing enzyme buffer liquid
(0.02MTris HCl/0.001MEDTA, pH7.0 solution) is diluted to protein content 5-10mg/ml.Enzyme dilution is molten with PB
Liquid (2.0mol/L potassium dihydrogen phosphate, pH7.5) mixes in equal volume, and (10 milligrams of epoxy type fixed enzyme vector LX-3000 is added
Enzyme/gram carrier), it is reacted 20 hours for 25 DEG C in shaking table (revolving speed 100rpm).Sock filtration is used after the reaction was completed, with washing enzyme buffer
Liquid cleans 5-6 times, obtains immobilization nicotinamide-nucleotide adenylyltransferase.
Nicotinamide adenine dinucleotide is prepared with immobilization nicotinamide-nucleotide adenylyltransferase
Prepare substrate solution: trinosin (ATP), the 100mMTris of nicotinamide nucleotide, 10mM containing 5mM
The MgCl2 of hydrochloride buffer and final concentration of 10mM adjusts pH to 7.5.1 milliliter of substrate solution is taken, 0.05 gram of fixation is then added
Change nicotinamide-nucleotide adenylyltransferase, reaction 2-20 hours is carried out in 37 DEG C.Centrifugation (10 DEG C, 17,800g, 15 minutes) is simultaneously received
Collect supernatant.The content of nicotinamide adenine dinucleotide in the supernatant as obtained by high performance liquid chromatography (HPLC) measurement.Knot
Fruit, the conversion ratio that nicotinamide nucleotide is converted into nicotinamide adenine dinucleotide is more than 80%.
The present embodiment also proposed the above-mentioned composition containing NADH and alleviate in preparation and/or reduce in the beverage of fatigue
Using.
For example, the present embodiment proposes that a kind of energy extender of composition containing NADH, the energy extender pass through
Following steps preparation:
S1, each component in composition is separately dried, is crushed, crosses 30 meshes, keeping distribution of particle sizes uniform, mobility is more
It is good, it weighs and is uniformly mixed in each component ratio；
In S2, the mixture obtained to step S1 plus the water of 5 times of amounts stirs and evenly mixs, and is uniformly mixed at 25 DEG C, so
After be warming up to 50 DEG C after extract 1h, obtain the mixture containing NADH.
S3, the mixture in step S2 is filtered, obtains leaching liquor, leaching liquor be in S2 plus the volume of water 2/5；
S4, the leaching liquor and flavoring agent appropriate that step S3 is added in the boiling water of 5 times of volumes of leaching liquor, are uniformly mixed,
Maintain the temperature at 50 DEG C, homogenization 2 times, when the finer words of solution, enhance the stablizing effect of solution, to the solution after homogeneous
It is de-gassed, sterilizes, is canned, obtaining the energy extender of the composition containing NADH.
In the present embodiment, suitable flavoring agent such as white sugar, honey, edible salt etc., the present invention couple can be added according to mouthfeel
This is not specifically limited.
Energy extender provided in this embodiment rich in NADH composition, which not only meets in nutritional ingredient and content, to be adapted to
Movement or physical exertion crowd physilogical characteristics, moreover it is possible to by rapid osmotic absorption, reach keep the skin wet, the effect of electrolyte and energy
Fruit makes mobilization quickly regain one's strength.
Embodiment 2
The present embodiment provides a kind of compositions containing NADH, and in parts by weight, the composition includes 5 parts of NADH, 7 parts
Co-Q10,1 part of chondroitin sulfate, 9 parts of ursodesoxycholic acid, 1 part of glycosyl isoflavones, 10 parts of s- Ademetionines, 5 parts
γ-aminobutyric acid, 10 parts of PPD and 7 part of Rh2；And auxiliary material, auxiliary material include 0.01 part of sodium chloride, 0.01 part of potassium chloride and
0.005 part of vitamin B12.
The present embodiment also proposed the above-mentioned composition containing NADH and alleviate in preparation and/or reduce in the beverage of fatigue
Using.
For example, the present embodiment proposes that a kind of energy extender of composition containing NADH, the energy extender pass through
Following steps preparation:
S1, by NADH, Co-Q10, chondroitin sulfate, ursodesoxycholic acid, the isoflavones of glycosyl, s- adenosine egg ammonia
Acid, γ-aminobutyric acid, PPD and Rh2 are separately dried, crush, crossing 50 meshes, keep distribution of particle sizes uniform, and mobility is more preferable,
And weighing mixing in proportion；
In S2, the mixture obtained to step S1 plus the water of 8 times of amounts stirs and evenly mixs, and is uniformly mixed at 25 DEG C, so
After be warming up to 56 DEG C after extract 1.5h, obtain the mixture containing NADH.
S3, the mixture in step S2 is filtered, obtains leaching liquor, leaching liquor be in S2 plus the volume of water 3/5；
S4, the leaching liquor that step S3 is added in the boiling water of 15 times of volumes of leaching liquor, the water measured in advance with 0.5 times dissolve
Auxiliary material and flavoring agent appropriate, be uniformly mixed, maintain the temperature at 55 DEG C, homogenization 2 times, when the finer words of solution, increase
The stablizing effect of strong solution, is de-gassed the solution after homogeneous, sterilizes, is canned, obtaining the energy of the composition containing NADH
Replenishers.
Embodiment 3
The present embodiment provides a kind of compositions containing NADH, and in parts by weight, the composition includes 7 parts of NADH, 8 parts
Co-Q10,4 parts of chondroitin sulfates, 6 parts of ursodesoxycholic acid, 3 parts of glycosyls isoflavones, 10 parts of s- Ademetionines, 4 parts
γ-aminobutyric acid, 1 part of PPD and 10 part of Rh2；And auxiliary material, auxiliary material include 0.05 part of sodium chloride, 0.003 part of vitamin B12,
0.01 part of vitamin C, 0.004 part of vitamin B6,0.05 part of taurine and 0.009 part of magnesium chloride.
The present embodiment also proposed the above-mentioned composition containing NADH and alleviate in preparation and/or reduce in the beverage of fatigue
Using.
For example, the present embodiment proposes that a kind of energy extender of composition containing NADH, the energy extender pass through
Following steps preparation:
S1, by NADH, Co-Q10, chondroitin sulfate, ursodesoxycholic acid, the isoflavones of glycosyl, s- adenosine egg ammonia
Acid, γ-aminobutyric acid, PPD and Rh2 are separately dried, crush, crossing 40 meshes, keep distribution of particle sizes uniform, and mobility is more preferable,
And weighing mixing in proportion；
In S2, the mixture obtained to step S1 plus the water of 10 times of amounts stirs and evenly mixs, and is uniformly mixed at 40 DEG C, so
After be warming up to 60 DEG C after extract 2h, obtain the mixture containing NADH.
S3, the mixture in step S2 is filtered, obtains leaching liquor, leaching liquor be in S2 plus the volume of water 1/2；
S4, the leaching liquor that step S3 is added in the boiling water of 40 times of volumes of leaching liquor, the water measured in advance with 5 times have dissolved
Auxiliary material and flavoring agent appropriate are uniformly mixed, maintain the temperature at 60 DEG C, homogenization 2 times, when the finer words of solution, enhancing
The stablizing effect of solution is de-gassed the solution after homogeneous, sterilizes, is canned, and the energy for obtaining the composition containing NADH is mended
Fill agent.
Embodiment 4
The present embodiment provides a kind of compositions containing NADH, and in parts by weight, the composition includes 10 parts of NADH, 10
Part Co-Q10,10 parts of chondroitin sulfates, 1 part of ursodesoxycholic acid, 7 parts of glycosyls isoflavones, 6 parts of s- Ademetionines, 10
Part γ-aminobutyric acid, 3 parts of PPD and 10 part of Rh2；And auxiliary material, auxiliary material include 0.02 part of sodium chloride, 0.03 part of potassium chloride,
0.002 part of vitamin B12,0.005 part of vitamin C, 0.002 part of vitamin B6,0.03 part of taurine and 0.001 part of chlorination
Magnesium.
The present embodiment also proposed the above-mentioned composition containing NADH and alleviate in preparation and/or reduce in the beverage of fatigue
Using.
For example, the present embodiment proposes that a kind of energy extender of composition containing NADH, the energy extender pass through
Following steps preparation:
S1, by NADH, Co-Q10, chondroitin sulfate, ursodesoxycholic acid, the isoflavones of glycosyl, s- adenosine egg ammonia
Acid, γ-aminobutyric acid, PPD and Rh2 are separately dried, crush, crossing 80 meshes, keep distribution of particle sizes uniform, and mobility is more preferable,
And weighing mixing in proportion；
In S2, the mixture obtained to step S1 plus the water of 20 times of amounts stirs and evenly mixs, and is uniformly mixed at 40 DEG C, so
After be warming up to 54 DEG C after extract 3h, obtain the mixture containing NADH.
S3, the mixture in step S2 is filtered, obtains leaching liquor, leaching liquor be in S2 plus the volume of water 1/5；
S4, the leaching liquor that step S3 is added in the boiling water of 30 times of volumes of leaching liquor, the water measured in advance with 0.8 times dissolve
Auxiliary material and flavoring agent appropriate, be uniformly mixed, maintain the temperature at 54 DEG C, homogenization 2 times, when the finer words of solution, increase
The stablizing effect of strong solution, is de-gassed the solution after homogeneous, sterilizes, is canned, obtaining the energy of the composition containing NADH
Replenishers.
Embodiment 5
The present embodiment provides a kind of compositions containing NADH, and in parts by weight, the composition includes 3 parts of NADH, 4 parts
Co-Q10,5 parts of chondroitin sulfates, 10 parts of ursodesoxycholic acid, 5 parts of glycosyls isoflavones, 8 parts of s- Ademetionines, 8 parts
γ-aminobutyric acid, 7 parts of PPD and 5 part of Rh2；And auxiliary material, auxiliary material include 0.04 part of sodium chloride, 0.05 part of potassium chloride, 0.004
Part vitamin B12,0.008 part of vitamin C, 0.004 part of vitamin B6,0.04 part of taurine and 6 parts of honey.
The present embodiment also proposed the above-mentioned composition containing NADH and alleviate in preparation and/or reduce in the beverage of fatigue
Using.
For example, the present embodiment proposes that a kind of energy extender of composition containing NADH, the energy extender pass through
Following steps preparation:
S1, by NADH, Co-Q10, chondroitin sulfate, ursodesoxycholic acid, the isoflavones of glycosyl, s- adenosine egg ammonia
Acid, γ-aminobutyric acid, PPD and Rh2 are separately dried, crush, crossing 80 meshes, keep distribution of particle sizes uniform, and mobility is more preferable,
And weighing mixing in proportion；
In S2, the mixture obtained to step S1 plus the water of 18 times of amounts stirs and evenly mixs, and is uniformly mixed at 30 DEG C, so
After be warming up to 51 DEG C after extract 2.5h, obtain the mixture containing NADH.
S3, the mixture in step S2 is filtered, obtains leaching liquor, leaching liquor be in S2 plus the volume of water 3/10；
S4, the leaching liquor that step S3 is added in the boiling water of 25 times of volumes of leaching liquor, the water measured in advance with 1 times have dissolved
Auxiliary material and flavoring agent appropriate are uniformly mixed, maintain the temperature at 51 DEG C, homogenization 2 times, when the finer words of solution, enhancing
The stablizing effect of solution is de-gassed the solution after homogeneous, sterilizes, is canned, and the energy for obtaining the composition containing NADH is mended
Fill agent.
Experimental example-energy extender Activity determination
(1) zoopery
Choose healthy male Kunming strain mice 180, weight (20 ± 2) g.It at random will growth be normal, is able to swim by weight
Experiment mice be divided into 3 groups: the NADH energy extender of experiment I group stomach-filling embodiment 2, the commercially available polypeptide type of 1 stomach-filling of control group
Sports drink, 2 stomach-filling physiological saline of control group, gastric infusion after every group 60, laboratory rearing 1 week, dosage press body surface area
It is 0.1mL10g-1, one time a day, continuous 21d that ratio, which converts equivalent dose,.During experiment, mouse freely ingests and drinks water.
(2) measurement of mouse growth rate
The weight of an experiment mice is respectively weighed before and after experiment, calculating averagely has a net increase of quality.It the results are shown in Table 1.
| Group | Quality/g before testing | Quality/g at the end of experiment | Averagely have a net increase of quality/g |
| I group | 20.78±1.08 | 35.58±1.09 | 14.8±0.52 |
| Control group 1 | 19.18±1.78 | 33.54±0.60 | 14.36±1.62 |
| Control group 2 | 20.11±1.92 | 35.84±0.60 | 15.73±1.30 |
Influence of 1 different disposal of table to mouse growth speed
As shown in Table 1,3 groups of intragastric administration on mice 21d test results show that I group and the mouse of control group 1,2 averagely have a net increase of quality
There was no significant difference.Therefore the energy extender containing NADH does not influence the growth and development of mouse body.
(3) energy extender safety detection
During 21d is fed, every ingest, drink water, stool and urine situation in 3 groups of mouse are observed, growth and development, row are observed
Whether normal it is, and whether there are poisoning manifestations, after experiment, checks liver, the heart, lung, kidney, jejunum, spleen of each test mice
Whether get damaged or lesion for dirty, thymus gland and testis.
The results show that during 21d is fed, the ingesting, drink water of 3 groups of mouse, stool and urine are normal, and growth and development is good, not
Observe that evident act changes and poisoning manifestations, dissects mouse to its liver, the heart, lung, kidney, jejunum, spleen, chest when off-test
Gland and testis carry out health examination, do not find obvious lesion, groups of animals internal organs are showed no the damaging as caused by tested material and change
Become, therefore the energy extender containing NADH composition is safe and non-toxic.
(4) mouse Burden-Swimming Ability of KM is tested
After mouse continuous gavage 21d, last stomach-filling 30min, swimming with a load attached to the body experiment is carried out.It bears a heavy burden at mouse tail 1/3
Quality is the sheet lead of 5% weight, puts into depth of water 30cm, 25-30 DEG C of water temperature of swimming trunk went swimming, and observation and record mouse enter certainly
The time that cannot be emerged to submerged 10s after water and (exhaust state in power).It the results are shown in Table 2.
| Group | Power exhausts swimming required time/min |
| I group | 56.0±2.1 |
| Control group 1 | 36.7±2.0 |
| Control group 2 | 30.8±1.8 |
Influence of energy extender of the table 2 containing NADH to the mice burden swimming time
Swimming with a load attached to the body laboratory is the animal model for evaluating antifatigue effect, and length and the exercise tolerance of swimming time have
It closes, the degree of animal movement fatigue can be reacted.In this test, mouse is given after different energy extender feeds, and right
It 1,2 is compared according to group, I group, the processing of IV group can significantly extend the mice burden swimming time, and II group, that III group has conspicuousness is poor
It is different, but there was no significant difference between this two groups, therefore, it is known that the energy extender containing NADH has certain antifatigue work
With.
(5) measurement of mouse biochemical indicator
Swimming power is exhausted rear mouse to pull out from water, the 0.5ml of eye socket blood sampling immediately separates serum, detects blood cream in serum
The content of sour (Lac), superoxide dismutase (SOD), malonaldehyde (MDA) and serum urea nitrogen (BUN).
Then experiment mice after blood sampling is put to death immediately.Liver is separated, is blotted after physiological saline rinses with filter paper, accurately
Liver 200mg is weighed, with the content of anthrone colorimetry measurement each group mouse hepatic glycogen.The results are shown in Table 3.
| Group | Lac% | Hepatic glycogen (mg) | SOD(u/ml) | MDA(mmol/ml) | BUN(mg/ml) |
| I group | 37.81±1.51 | 5.53±0.65 | 23.53±1.41 | 8.53±0.45 | 14.53±1.05 |
| Control group 1 | 43.39±1.78 | 3.83±0.61 | 15.56±1.34 | 9.16±0.61 | 19.13±1.12 |
| Control group 2 | 48.63±1.25 | 2.46±0.15 | 13.03±1.15 | 10.97±0.45 | 20.11±1.03 |
The measurement result of 3 mouse biochemical indicator of table
As seen from the above table, after to mouse continuous gavage 21d, Serum lactic acid content, the mda content of I group are low, and hepatic glycogen surpasses
Superoxide dismutase content is higher, and there are significant differences with control group 1 and control group 2.It is provided by the invention to contain NADH group
The energy extender for closing object has good synergistic effect, and each component is used in conjunction with, and produces the effect of synergy.
During exercise, muscle produces a large amount of lactic acid by glycolysis reaction, dissociates hydrogen ion generated by lactic acid
Increase the hydrogen ion concentration in muscle, pH value decline, and then cause a series of Biochemical changes, lead to fatigue, reduces lactic acid
Generation or accelerate removings of lactic acid that can cause antifatigue effect, malonaldehyde is that the sensitivity of measurement matrix Radical Metabolism refers to
Mark, content can levels that objectively reactive matrix generates free radicals.This is the experimental results showed that NADH energy extender can inhibit
Lipid peroxidation reduces lactic acid accumulation, the very fast effect for eliminating sports fatigue.Glycogen is most important energy substance in movement,
The level of internal glycogen is closely related with endurance, when the raising of glycogen reserve is conducive to the raising and movement of matrix endurance speed
Between extension.Superoxide dismutase within the organization can be excessive in removing machine body oxygen radical, body immunity can be improved, increase
Strong anti-oxidation ability, antioxidant have become a kind of important nourishing tonic for sport.Stomach-filling contains NADH composition in experiment
After energy extender, mouse superoxide dismutase content, glycogen content are dramatically increased, to prove to contain NADH composition
Energy extender has the oxidation resistance of enhancing mouse, eliminates the function of sports fatigue.Energy containing NADH composition is mended
Filling agent mechanism of action may be by the deposit of hepatic glycogen during increase exhausted movemeat, significantly reduce metabolite MDA, BUN
Level, improve SOD activity, accelerate the removing of free radical, enhance the oxidation resistance of mouse, mitigate the oxidative damage of matrix,
It improves the locomitivity of mouse, plays antifatigue effect.
The above is only a preferred embodiment of the present invention, protection scope of the present invention is not limited merely to above-mentioned implementation
Example, all technical solutions belonged under thinking of the present invention all belong to the scope of protection of the present invention.It should be pointed out that for the art
Those of ordinary skill for, several improvements and modifications without departing from the principles of the present invention, these improvements and modifications
It should be regarded as protection scope of the present invention.
Claims (10)
1. a kind of composition containing NADH, which is characterized in that including following parts by weight of component: 1-10 parts NADH, 1-10 parts auxiliary
Q10,1-10 parts of chondroitin sulfates of enzyme, 1-10 parts of ursodesoxycholic acid, 1-10 parts of glycosyls isoflavones, 1-10 portions of s- adenosine eggs
Propylhomoserin, 1-10 parts of γ-aminobutyric acids, 1-10PPD and 1-10 parts of Rh2.
2. as described in claim 1 containing the composition of NADH, which is characterized in that further include auxiliary material, the auxiliary material include with
At least one of lower parts by weight of component: 0.01-0.05 parts of sodium chloride, 0.01-0.05 parts of potassium chloride, 0.001-0.005 parts of dimension lifes
Plain B12,0.001-0.01 part of vitamin C, 0.001-0.005 parts of vitamin B6s, 0.02-0.05 parts of taurines, 3-6 parts of honey,
0.005-0.01 parts of magnesium chlorides.
3. as claimed in claim 2 containing the composition of NADH, which is characterized in that the auxiliary material includes following parts by weight of component
At least five kinds: 0.01-0.05 part of sodium chloride, 0.01-0.05 parts of potassium chloride, 0.001-0.005 parts of vitamin B12s, 0.001-
0.01 part of vitamin C, 0.001-0.005 parts of vitamin B6s, 0.02-0.05 parts of taurines, 3-6 parts of honey, 0.005-0.01 parts
Magnesium chloride.
4. the beverage of fatigue is alleviated and/or reduced to the composition as described in any one of claims 1-3 containing NADH in preparation
In application.
5. a kind of energy extender of the composition containing NADH, which is characterized in that including as described in claim any one of 1-4
The composition containing NADH.
6. a kind of method for the energy extender for preparing the composition described in claim 5 containing NADH, which is characterized in that packet
Include following steps:
S1, by NADH, Co-Q10, chondroitin sulfate, ursodesoxycholic acid, the isoflavones of glycosyl, s- Ademetionine, γ-
Aminobutyric acid, PPD and Rh2 are separately dried, crush, crossing 30-80 mesh, and weighing mixing in proportion；
S2, the raw material into step S1 are added water and stir and evenly mix, and then extract 1-3h at 50-60 DEG C, obtain mixture；
S3, the mixture in step S2 is filtered, obtains leaching liquor；
S4, the leaching liquor that step S3 is added in boiling water and flavoring agent, auxiliary material are uniformly mixed, and maintain the temperature at 50-60 DEG C, homogeneous
It handles, the solution after homogeneous is de-gassed, sterilizes, is canned, obtains the energy extender of the composition containing NADH.
7. the method for the energy extender of composition of the preparation containing NADH as claimed in claim 6, which is characterized in that described
The mass ratio of raw material and water in step S2 is 1:5-10.
8. the method for the energy extender of composition of the preparation containing NADH as claimed in claim 7, which is characterized in that described
The 1/5-3/5 of leaching liquor obtained in step S3 and the volume that water is added in step S2.
9. the method for the energy extender of composition of the preparation containing NADH as claimed in claim 8, which is characterized in that described
It after auxiliary material in step S4 is dissolved completely with water, adds in boiling water, the mass ratio of the auxiliary material and water is 1:0.5-5.
10. the method for the energy extender of composition of the preparation containing NADH as claimed in claim 9, which is characterized in that institute
State the leaching liquor in step S4: the volume ratio of boiling water is 1:5-40.
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| CN115299534A (en) * | 2022-09-14 | 2022-11-08 | 泓博元生命科技（深圳）有限公司 | NADH composition for quickly supplementing energy and relieving fatigue of pets and preparation method thereof |
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