CN109090341A - A kind of solid-state multifunctional enzyme hydrolysis and fermentation technique - Google Patents
A kind of solid-state multifunctional enzyme hydrolysis and fermentation technique Download PDFInfo
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- CN109090341A CN109090341A CN201810978757.6A CN201810978757A CN109090341A CN 109090341 A CN109090341 A CN 109090341A CN 201810978757 A CN201810978757 A CN 201810978757A CN 109090341 A CN109090341 A CN 109090341A
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Abstract
The present invention provides a kind of solid-state multifunctional enzyme hydrolysis and fermentation techniques, belong to technical field of biological fermentation.It solves that existing zymotechnique integrated synthesis function is weaker, and zymotechnique is not perfect, leads to the problems such as fermentation period is long, and treatment effeciency is low, poor processing effect.This solid-state multifunctional enzyme hydrolysis and fermentation technique passes through: sterilizing in place and cooling charging, air filtration, mix, after the processes such as reality disappears, detects, compensates, cools down, is inoculated with, after fermentation in 6~12 hours, the pH value detected by the sensing detection device is 4.0~5.0, has sour taste, after detection reaches the innoxious numerical indication of biological feedstuff, exhausting system, continuous stirring system are opened, to realize the low temperature drying of material, compound micro-ecological preparation is obtained by screening.The present invention has many advantages, such as high degree of automation, ambient stable.
Description
Technical field
The invention belongs to technical field of biological fermentation, are related to a kind of zymotechnique, in particular to a kind of solid-state multifunctional enzyme
Hydrolysis and fermentation technique.
Background technique
China is feeding amount big country, and with the continuous development and expansion of cultivation scale, feedstuff has occurred in China
The situation of shortage, and be possible to become the bottlenecks of animal husbandry development from now on, feed demand is from 1.37 hundred million in 2008
Ton increases to 1.94 hundred million tons in 2012, increases 41.6%.In contrast, China's grain yield increasing degree is relatively low.
However, the cultivated area in China is reduced year by year as China's economic development and urbanization promote.The arable land in China in 2012
Area is 1,217,300,000,000 m2, has been approached this arable land red line of 1,200,000,000,000 m2 of country's promulgation in 2007.China's feedstuff it is short
The development and utilization that scarce problem has become China's unconventional water resources resource is constantly subjected to government, researcher and feed manufacturer
Attention.
Microorganism solid fermentation is widely applied with its unique advantage in herding, food, medicine and other fields, and existing hair is become
The mainstream of ferment technology occurs much being directed to wheat bran, maize flour, powdered rice hulls and flower both at home and abroad especially in terms of feed processing
Fecula etc. carry out mixed fermentation processing equipment, relative to natural packing ferment, have many advantages, such as that fermentation period is short, pollute it is small,
But still there are following main problems: first is that Zymolysis Equipment is expensive, operating cost is high;Second is that integrated synthesis function compared with
Weak, zymotechnique is not perfect, causes fermentation period long, and treatment effeciency is low, poor processing effect.
Summary of the invention
The purpose of the present invention is being directed to the above-mentioned problems in the prior art, a kind of multi-functional enzymatic hydrolysis hair of solid-state is provided
Ferment technique, the technique provide sterile, stable enzymatic hydrolysis environment for fermentation process.
Object of the invention can be realized by the following technical scheme: a kind of solid-state multifunctional enzyme hydrolysis and fermentation technique, including
Following steps:
1) sterilizing and cooling in place: being passed through steam, and steam guarantees sterile yeasting through filtering, crosses filtration after opening of device
Vapour is preheated to steam is passed through in external jacket, and after preheating is completed, steam is passed directly into fermentor.
2) it feeds: selected fermentation substrate and fermentation auxiliary material, by fermentation substrate and fermentation auxiliary material with the ratio of 40~70:30~50
Example is sufficiently mixed, and is put into enzymatic hydrolysis and fermentation tank, and mixture volume is no more than the 70% of Zymolysis Equipment volume, is sufficiently stirred
It mixes;
3) air filtration: pure air is converted by filtrated air by introducing purification device and enters solid enzymatic hydrolysis fermentation
Tank;
4) it mixes: 2) the middle air introduced and fermentation substrate, fermentation auxiliary material is sufficiently mixed, and adjusts moisture in mixture
Content, make water content overall control 40~60%;
5) real to disappear: 4) mixture in be heated, material is heated to 70~80 DEG C, and high temperature continues 25~35 minutes height
Warm sterilizing in place;
6) it detects: detecting the pH value and DO of fermenting mixture by the sensing detection device being built in enzymatic hydrolysis and fermentation tank
Value;
7) it compensates: pH value being adjusted by addition acid reagent or base reagent on the basis of 6), so that fermenting mixture is whole
Ph when control between 4.0~5.0;The fermentation of multi-cultur es solid phase dynamic circulation, it is different according to each strain fermentation condition, it needs to set
Surely suitable fermentation temperature, oxygen supply time and oxygen-supplying amount, thus realize oxygen consumption-anaerobism-oxygen consumption-anaerobism alternately, it is dynamic
The Fermentation Function of state circulation, realizes the fermentation of multi-cultur es solid phase dynamic equalization, and the Oxygen control of multi-cultur es solid phase dynamic equalization passes through
DO compensation device carries out DO compensation, oxygen feeding stop when anaerobic state, and oxygen-supplying amount is (9m3/min) when aerobic.
8) cooling: cooling to be combined using collet water-cooling circulating system with air cooling system, system is cooled to 30~45 DEG C;
9) be inoculated with: inoculation is atomized vaccination ways using pressure difference in place, in 8) investment mixture gross mass 0.1%~
0.2% feeding probiotics bacterial liquid, is sufficiently stirred mixing, is placed in uniform stirring post-fermentation at 30~37 DEG C;
10) after fermentation in 6~12 hours, the pH value detected by the sensing detection device is 4.0~5.0, has sour taste,
After detection reaches the innoxious numerical indication of biological feedstuff, exhausting system, continuous stirring system are opened, to realize the low of material
Temperature is dry, obtains compound micro-ecological preparation by screening.
In a kind of above-mentioned solid-state multifunctional enzyme hydrolysis and fermentation technique, sensing detection device includes controller and by controller
The PH sensor and DO sensor of control, the PH sensor are electrically connected with make-up pump so that PH sensor, make-up pump,
A PH feedback system is formed between controller, the DO sensor is electrically connected with DO compensation device, so that DO sensor, DO
A DO feedback system is formed between compensation device and controller.
In a kind of above-mentioned solid-state multifunctional enzyme hydrolysis and fermentation technique, introducing purification device includes air compressor machine and and air compressor machine
The air reservoir being connected, the air reservoir are connected with enzymatic hydrolysis and fermentation tank, and air reservoir and enzymatic hydrolysis and fermentation tank it
Between be provided with filter device.
In a kind of above-mentioned solid-state multifunctional enzyme hydrolysis and fermentation technique, the filter device is connected with air reservoir device
Pressure reducing valve and gs-oil separator, be connected in series between the pressure reducing valve and gs-oil separator filter device and air reservoir device it
Between.
In a kind of above-mentioned solid-state multifunctional enzyme hydrolysis and fermentation technique, the filter device includes total filter, pre-filtering
Device and fine filter are mutually contacted between the total filter, prefilter and fine filter, and total filter one end and oil
Separator is connected.
In a kind of above-mentioned solid-state multifunctional enzyme hydrolysis and fermentation technique, the enzymatic hydrolysis and fermentation tank is circumscribed with steam unit, institute
The steam unit one end and enzymatic hydrolysis and fermentation tank stated are direct-connected, and the other end is connected with fine filter, the fine filter and steaming
Vapor filter is provided between vapour device for transition.
The material of fermentation substrate in a kind of above-mentioned solid-state multifunctional enzyme hydrolysis and fermentation technique, sorting step 2) can be bran
Skin, maize flour, powdered rice hulls and peanut powder weight ratio 4:1:3:2 mixture, another equipment processing material further includes agricultural and sideline poor slag
(vinasse, sugar residue, fecula, manioc waste, pomace etc.), feces of livestock and poultry, miscellaneous Hectometer and agricultural crop straw class plant source property waste.
In a kind of above-mentioned solid-state multifunctional enzyme hydrolysis and fermentation technique, enzymolysis step 9) the wherein described feeding probiotics is excrement
Enterococcus, S. cervisiae, bacillus subtilis, protease and cellulase mixture.1~2:2 of its mass ratio~4:1~2:
1~0.2, preferably 1:2:1:0.2.
In a kind of above-mentioned solid-state multifunctional enzyme hydrolysis and fermentation technique, enzymolysis step 9) in order to guarantee fermented quality, preferably adopt
With the stoste (compound bacterias such as enterococcus faecalis, S. cervisiae, bacillus subtilis) of the feeding probiotics of submerged fermentation or will be prebiotic
Bacterium fermenting agent and etc. weight glucose activated 30 minutes with warm water, be pumped into fermentor, then by material described in step
The 0.1~0.2% of weight is added in material, mixes.
Compared with prior art, this solid-state multifunctional enzyme hydrolysis and fermentation technique is led to relative to traditional solid-state enzyme fermentation technique
It crosses introducing purification device to pre-process the air that enzymatic hydrolysis and fermentation tank is added, detection is then provided with after the heating period, is mended
The processes such as repay, entire Fermentation Engineering after zymotechnique reasonably optimizing there is yeasting to stablize, automatically all in sealed environment
The advantages that change degree is high.
Detailed description of the invention
Fig. 1 is the overall construction drawing that purification device is introduced in this solid-state multifunctional enzyme hydrolysis and fermentation technique.
Fig. 2 is the part working principle diagram of sensing detection device in this solid-state multifunctional enzyme hydrolysis and fermentation technique.
Fig. 3 is the pipe-line system flow chart of this solid-state multifunctional enzyme hydrolysis and fermentation technique.
In figure, 1, controller;2, PH sensor;3, DO sensor;4, make-up pump;5, DO compensation device;6, air compressor machine;7,
Air reservoir;8, filter device;9, pressure reducing valve;10, gs-oil separator;11, total filter;12, prefilter;13, essence filtering
Device;14, steam unit;15, vapor filter.
Specific embodiment
Following is a specific embodiment of the present invention in conjunction with the accompanying drawings, technical scheme of the present invention will be further described,
However, the present invention is not limited to these examples.
In conjunction with shown in Fig. 1, Fig. 2, Fig. 3, a kind of this solid-state multifunctional enzyme hydrolysis and fermentation technique the following steps are included:
1) sterilizing and cooling in place: state-of-the-art sterile vapor filtration system is used, steam is filtered by pre-filtering and essence
Double-filtration guarantees sterile yeasting, and filtering steam is preheated to steam is passed through in external jacket after opening of device, preheat
After completion, steam is passed directly into fermentor.Solids is eliminated compared with traditional rotating disc type, cultivated slot type solid fermentation apparatus
Expect a large amount of ancillary equipments such as steaming stock pot, sterile material transportation system.It is cooling to be combined using collet recirculated water with ventilation, it adjusts simultaneously
The moisture content of material after section sterilizing.
2) it feeds: selected fermentation substrate and fermentation auxiliary material, by fermentation substrate and fermentation auxiliary material with the ratio of 40~70:30~50
Example is sufficiently mixed, and is put into enzymatic hydrolysis and fermentation tank, and mixture volume is no more than the 70% of Zymolysis Equipment volume, is sufficiently stirred
It mixes;
3) air filtration: using state-of-the-art filtrated air filtration system, fermentation process using full-automatic control system into
Row control is to collect machinery and control integrated equipment.Pure air is compressed to air reservoir by air compressor machine, is decompressed to
Air total filter and air fine filter are transported to after 0.30Mpa, filtrated air out enters solid enzymatic hydrolysis fermentor.
Air and steam are required to pre-filtering and essence filtering double-filtration to guarantee gnotobasis;
4) it mixes: 2) the middle air introduced and fermentation fermentation substrate, fermentation auxiliary material is sufficiently mixed, and adjusted in mixture
The content of moisture makes water content overall control 40~60%;
5) real to disappear: 4) mixture in be heated, material is heated to 70~80 DEG C, and high temperature continues 25~35 minutes height
Warm sterilizing in place;
6) it detects: detecting the pH value and DO of fermenting mixture by the sensing detection device being built in enzymatic hydrolysis and fermentation tank
Value;
7) it compensates: acid reagent being added by make-up pump on the basis of 6) or base reagent adjusts pH value, so that fermentation mixing
Control is between 4.0~5.0 when the ph of object entirety;The fermentation of multi-cultur es solid phase dynamic circulation, it is different according to each strain fermentation condition,
Need to set suitable fermentation temperature, oxygen supply time and oxygen-supplying amount, to realize oxygen consumption-anaerobism-oxygen consumption-anaerobism alternating
It carries out, the Fermentation Function of dynamic circulation, realizes the fermentation of multi-cultur es solid phase dynamic equalization.The oxygen control of multi-cultur es solid phase dynamic equalization
System carries out DO compensation by compensation device, oxygen feeding stop when anaerobic state, and oxygen-supplying amount is (9m3/min) when aerobic.
8) cooling: cooling to be combined using collet water-cooling circulating system with air cooling system, system is cooled to 30~45 DEG C;
9) be inoculated with: inoculation is atomized vaccination ways using pressure difference in place, in 8) investment mixture gross mass 0.1%~
0.2% feeding probiotics bacterial liquid, is sufficiently stirred mixing, is placed in uniform stirring post-fermentation at 30~37 DEG C;
10) after fermentation in 6~12 hours, the pH value detected by the sensing detection device is 4.0~5.0, has sour taste,
After detection reaches the innoxious numerical indication of biological feedstuff, roots blower, top exhausting system, continuous stirring system are opened, from
And realize the low temperature drying of material, compound micro-ecological preparation is obtained by screening.
Further describe in detail, sensing detection device include controller 11 and by controller 11 control PH sensor 2 and
DO sensor 3, PH sensor 2 are electrically connected with make-up pump 4, so that forming one between PH sensor 2, make-up pump 4, controller 11
A PH feedback system, DO sensor 3 are electrically connected with DO compensation device 5, so that DO sensor 3, DO compensation device 5 and controller 11
Between formed a DO feedback system.
It further describes in detail, introducing purification device includes air compressor machine 6 and the air reservoir being connected with air compressor machine 67, air
Storage tank 7 is connected with enzymatic hydrolysis and fermentation tank, and filter device 8 is provided between air reservoir 7 and enzymatic hydrolysis and fermentation tank.
It further describes in detail, filter device 8 and 7 device of air reservoir are connected with pressure reducing valve 9 and gs-oil separator 10, pressure reducing valve
It is connected in series between 9 and gs-oil separator 10 between 7 device of filter device 8 and air reservoir.
Further to describe in detail, filter device 8 includes total filter 11, prefilter 12 and fine filter 13, total filter 11,
It mutually contacts between prefilter 12 and fine filter 13, and 11 one end of total filter is connected with oil water separator.
Further to describe in detail, enzymatic hydrolysis and fermentation tank is circumscribed with steam unit 14, and 14 one end of steam unit and enzymatic hydrolysis and fermentation tank are direct-connected,
Its other end is connected with fine filter 13, and vapor filter 15 is provided between fine filter 13 and steam unit 14 and was used for
It crosses.
The technical effect of this solid-state multifunctional enzyme hydrolysis and fermentation technique are as follows: put into fermentation substrate in enzymatic hydrolysis and fermentation tank and fermentation is auxiliary
After material, pure air is compressed to air reservoir by the air compressor machine 6 being introduced into purification device, is transported to after being decompressed to 0.30Mpa
Air total filter 11 and air fine filter 13, filtrated air out enter enzymatic hydrolysis and fermentation tank, and air and steam are both needed to
Pre-filtering and essence filtering double-filtration is wanted to guarantee gnotobasis, after mixing and heating stepses, PH sensor 2 passes through pH
Electrode detection, and connect using signal transmssion line with PLC, it is ensured that pH is accurately controlled, and 2 road peristaltic pump of microcomputer-based intelligent PID control is certainly
Dynamic addition acid or alkali, so that pH value control, in set range, DO sensor 3 is connect using signal transmssion line with PLC, it is ensured that
DO accurately shows that system realizes on-line checking and the control of DO value with DO abnormal alarm function with this, passes through PH compensation and DO
Compensation guarantee the stability of environment during enzymatic hydrolysis and fermentation in enzymatic hydrolysis and fermentation tank, the quality of enzymolysis product is ensured with this.
Specific embodiment described herein is only an example for the spirit of the invention.The neck of technology belonging to the present invention
The technical staff in domain can make various modifications or additions to the described embodiments or replace by a similar method
In generation, however, it does not deviate from the spirit of the invention or beyond the scope of the appended claims.
Although be used more herein controller 1, PH sensor 2, DO sensor 3, make-up pump 4, DO compensation device 5,
Air compressor machine 6, air reservoir 7, filter device 8, pressure reducing valve 9, gs-oil separator 10, total filter 11, prefilter 12, essence filtering
The terms such as device 13, steam unit 14, vapor filter 15, but it does not exclude the possibility of using other terms.Use these arts
Language is only for the convenience of describing and explaining the nature of the invention;Being construed as any additional limitation is all
It is disagreed with spirit of that invention.
Claims (9)
1. a kind of solid-state multifunctional enzyme hydrolysis and fermentation technique, comprising the following steps:
1) sterilizing and cooling in place: being passed through steam, and steam guarantees sterile yeasting through filtering, filters steam pair after opening of device
It is passed through steam in external jacket to be preheated, after preheating is completed, steam is passed directly into fermentor.
2) feed: selected fermentation substrate and fermentation auxiliary material, by fermentation substrate and fermentation auxiliary material with the ratio of 40~70:30~50 into
Row is sufficiently mixed, and is put into enzymatic hydrolysis and fermentation tank, and mixture volume is no more than the 70% of Zymolysis Equipment volume, is sufficiently stirred mixed
It is even;
3) air filtration: pure air is converted by filtrated air by introducing purification device and enters solid enzymatic hydrolysis fermentor;
4) it mixes: 2) air introduced in and fermentation substrate, the auxiliary material that ferments is sufficiently mixed, and adjust containing for moisture in mixture
Amount, makes water content overall control 40~60%;
5) real to disappear: 4) mixture in be heated, material is heated to 70~80 DEG C, and high temperature continues 25~35 minutes high temperature and exists
Position sterilizing;
6) it detects: detecting the pH value and DO value of fermenting mixture by the sensing detection device being built in enzymatic hydrolysis and fermentation tank;
7) it compensates: pH value being adjusted by addition acid reagent or base reagent on the basis of 6), so that the ph of fermenting mixture entirety
When control between 4.0~5.0;The fermentation of multi-cultur es solid phase dynamic circulation, it is different according to each strain fermentation condition, it needs to set suitable
Suitable fermentation temperature, oxygen supply time and oxygen-supplying amount, to realize that oxygen consumption-anaerobism-oxygen consumption-anaerobism alternately, is dynamically followed
The Fermentation Function of ring, realizes the fermentation of multi-cultur es solid phase dynamic equalization, and the Oxygen control of multi-cultur es solid phase dynamic equalization passes through compensation
Device carries out DO compensation, oxygen feeding stop when anaerobic state, and oxygen-supplying amount is (9m3/min) when aerobic.
8) cooling: cooling to be combined using collet water-cooling circulating system with air cooling system, system is cooled to 30~45 DEG C;
9) be inoculated with: inoculation is atomized vaccination ways using pressure difference in place, in 8) middle investment mixture gross mass 0.1%~0.2%
Mixing is sufficiently stirred in feeding probiotics bacterial liquid, is placed in uniform stirring post-fermentation at 30~37 DEG C;
10) after fermentation in 6~12 hours, the pH value detected by the sensing detection device is 4.0~5.0, has sour taste, through examining
After survey reaches the innoxious numerical indication of biological feedstuff, exhausting system, continuous stirring system are opened, to realize that the low temperature of material is dry
It is dry, compound micro-ecological preparation is obtained by screening.
2. a kind of solid-state multifunctional enzyme hydrolysis and fermentation technique according to claim 1, which is characterized in that sensing detection device packet
Include controller (1) and PH sensor (2) and DO sensor (3) by controller (1) control, the PH sensor (2)
Make-up pump (4) are electrically connected with, so that a PH feedback system is formed between PH sensor (2), make-up pump (4), controller (1),
The DO sensor (3) is electrically connected with DO compensation device (5), so that DO sensor (3), DO compensation device (5) and controller
(1) a DO feedback system is formed between.
3. a kind of solid-state multifunctional enzyme hydrolysis and fermentation technique according to claim 1, which is characterized in that introduce purification device packet
The air reservoir (7) for including air compressor machine (6) and being connected with air compressor machine (6), the air reservoir (7) and enzymatic hydrolysis and fermentation tank phase
Connection, and filter device (8) are provided between air reservoir (7) and enzymatic hydrolysis and fermentation tank.
4. a kind of solid-state multifunctional enzyme hydrolysis and fermentation technique according to claim 3, which is characterized in that the filter device
(8) pressure reducing valve (9) and gs-oil separator (10), the pressure reducing valve (9) and Oil-gas Separation are connected with air reservoir (7) device
It is connected in series between device (10) between filter device (8) and air reservoir (7) device.
5. a kind of solid-state multifunctional enzyme hydrolysis and fermentation technique according to claim 3, which is characterized in that the filter device
It (8) include total filter (11), prefilter (12) and fine filter (13), the total filter (11), prefilter
(12) it mutually contacts between fine filter (13), and total filter (11) one end is connected with gs-oil separator (10).
6. a kind of solid-state multifunctional enzyme hydrolysis and fermentation technique according to claim 1, which is characterized in that enzymatic hydrolysis and fermentation tank is external
Have steam unit (14), the steam unit (14) one end and enzymatic hydrolysis and fermentation tank are direct-connected, the other end and fine filter (13)
It is connected, vapor filter (15) is provided between the fine filter (13) and steam unit (14) for transition.
7. a kind of solid-state multifunctional enzyme hydrolysis and fermentation technique according to claim 1, which is characterized in that in sorting step 2)
The material of fermentation substrate can for wheat bran, maize flour, powdered rice hulls and peanut powder weight ratio 4:1:3:2 mixture, at another equipment
Reason material further includes agricultural and sideline poor slag (vinasse, sugar residue, fecula, manioc waste, pomace etc.), feces of livestock and poultry, miscellaneous Hectometer and crops straw
Stalk class plants source property waste.
8. a kind of solid-state multifunctional enzyme hydrolysis and fermentation technique according to claim 1, which is characterized in that enzymolysis step 9) feeding
It is enterococcus faecalis, S. cervisiae, bacillus subtilis, protease and cellulase mixture with probiotics.Its mass ratio 1~
2:2~4:1~2:1~0.2, preferably 1:2:1:0.2.
9. a kind of solid-state multifunctional enzyme hydrolysis and fermentation technique according to claim 1, which is characterized in that enzymolysis step 9) in order to
Guarantee fermented quality, it is preferred to use stoste (enterococcus faecalis, S. cervisiae, the bacillus subtilis of the feeding probiotics of submerged fermentation
The compound bacterias such as bacterium) or by probiotics fermention microbial inoculum and etc. weight glucose with warm water activate 30 minutes, be pumped into fermentor, so
It is added in material by 0.1~0.2% of weight of material described in step afterwards, mixes.
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Citations (4)
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CN1057858A (en) * | 1991-08-14 | 1992-01-15 | 北京农业大学 | Increasing production bacteria solid fermentation process and special fermentation device thereof |
CN203986023U (en) * | 2014-07-02 | 2014-12-10 | 山东黎昊源生物工程有限公司 | Additive for microbe feedstuff production line |
CN106119073A (en) * | 2016-09-18 | 2016-11-16 | 江苏恒顺醋业股份有限公司 | A kind of intelligent vinegar solid brewing all-in-one |
CN107475106A (en) * | 2017-09-19 | 2017-12-15 | 北京艾吾丁医药科技有限公司 | A kind of fully-automatic intelligent fermentation system |
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Publication number | Priority date | Publication date | Assignee | Title |
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CN1057858A (en) * | 1991-08-14 | 1992-01-15 | 北京农业大学 | Increasing production bacteria solid fermentation process and special fermentation device thereof |
CN203986023U (en) * | 2014-07-02 | 2014-12-10 | 山东黎昊源生物工程有限公司 | Additive for microbe feedstuff production line |
CN106119073A (en) * | 2016-09-18 | 2016-11-16 | 江苏恒顺醋业股份有限公司 | A kind of intelligent vinegar solid brewing all-in-one |
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