CN109085696A - Active somatic cell batch transfer blade and preparation method thereof - Google Patents
Active somatic cell batch transfer blade and preparation method thereof Download PDFInfo
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- CN109085696A CN109085696A CN201811002454.7A CN201811002454A CN109085696A CN 109085696 A CN109085696 A CN 109085696A CN 201811002454 A CN201811002454 A CN 201811002454A CN 109085696 A CN109085696 A CN 109085696A
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- stainless steel
- steel substrates
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- somatic cell
- batch transfer
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- G—PHYSICS
- G02—OPTICS
- G02B—OPTICAL ELEMENTS, SYSTEMS OR APPARATUS
- G02B21/00—Microscopes
- G02B21/34—Microscope slides, e.g. mounting specimens on microscope slides
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- Analytical Chemistry (AREA)
- General Physics & Mathematics (AREA)
- Optics & Photonics (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
Abstract
Active somatic cell batch transfer blade and preparation method thereof, is related to cell biology and Hydrobiology.The active somatic cell batch transfer blade is equipped with stainless steel substrates and sheet glass, and the stainless steel substrates longitudinal stack bonds between stainless steel substrates and sheet glass on sheet glass.Stainless steel substrates are chosen, selection has the laser cutting machine of computer numerical control device, and copy cutting data to computer carries out Laser Oxygen lancing, after the completion of cutting, deburring, notch of polishing；Quartz glass plate is lain against on light-transmitting plate, A type stainless steel substrates are placed up regardless of front and back sides, the rectangular work ride of Type B stainless steel substrates, after AB glue is mixed, AB glue is applied on stainless steel substrates, then vertical pile is in quartz glass on piece, squeeze, alignment, etc. after AB glues are fully cured, wipe the extra glue in side off, then the glue of circular hole and square workspace because squeezing spilling, stainless steel substrates of polishing, polishing are removed, powder is removed, active somatic cell batch transfer blade is made.
Description
Technical field
The present invention relates to cell biologies and Hydrobiology, more particularly, to planktology field, such as: planktonic organism
Screening, purifying culture, Identification of Species, biological feed breeding, the screening of energy algae, the research of deposit packing etc., and in particular to
Active somatic cell batch transfer blade and preparation method thereof.
Background technique
Researcher often refers to active somatic cell screening, cleaning, transfer and cell image in planktonic organism is studied
The links such as acquisition, wherein during active somatic cell preliminary screening, because sample and cell surface adhesion have impurity (silt, deposition
Object clast, the relatively small non-aim cell of individual and bacterium etc.), it needs to carry out cell and cleans to keep cell relatively pure,
Usually selection water droplet method operation, i.e., it is spare to drip a few drop sterile waters on glass slide, under field of microscope, with capillary from thin
Born of the same parents separate in frame and draw cell, and cell is then transferred to water droplet, is repeated several times, realizes the cleaning of cell；Meanwhile for treasure
The cell dilute, culture is difficult carries out micro image collection, and in deposit packing research work, packing is sprouted, and initial only 1
Cell.In order to observe packing germination process, be also to take water droplet method, cell be transferred in water droplet, etc. after the completion of Image Acquisition,
Cell is transferred back to 96 orifice plates to continue to cultivate.
Water droplet partition method is a kind of more traditional cell sorting, cleaning and transfer method.This method is easy to operate, convenient,
It is skillfully grasped for vast planktonic organism researcher.Water droplet is transparent optical medium, in air since the effect of surface tension is in
Ellipsoid (being in sphere in agravic field), surface is curved surface.Water droplet method operates, and the volume of water droplet can be determined by pipettor,
But water droplet and glass slide contact area and water droplet thickness are by the factors shadow such as glass slide material, lustration class or even degree of drying
It rings, can not usually control.In addition, some cells can constantly move about in water droplet, or even swim over to water droplet edge, it is stationary,
Extremely difficult searching, is easily lost under field of microscope.Meanwhile when Image Acquisition, cell image is it sometimes appear that deformation, distortion.Separately
Outside, glass slide is generally not required to sterilize, and directly uses, easily entrains outer derived bacterium, increase the difficulty of cell culture.The above
Problem all gives planktonic organism researcher, especially phytoplankton researcher, causes certain puzzlement.
Summary of the invention
The purpose of the present invention is to provide active somatic cell batch transfer blades and preparation method thereof.
The active somatic cell batch transfer blade is equipped with stainless steel substrates and sheet glass, and the stainless steel substrates longitudinal stack is in glass
On piece, bonded between stainless steel substrates and sheet glass.
The high saturating quartz glass plate of ultrawhite can be used in the stainless steel substrates for band 316 stainless steel substrates of circular hole, the sheet glass,
Heatproof AB glue seamless adhesion can be used in the bonding；The specification of the stainless steel substrates can be long 63.94mm, wide 25.26mm, thickness
1mm；The specification of the sheet glass can be long 75.90mm, wide 25.30mm, thick 0.8mm；LEAFTOP/ indigo plant can be used in the AB glue
9940 heatproof AB glue of field；The Circularhole diameter of the stainless steel substrates may respectively be 5~9mm；
Circular hole on the stainless steel substrates can be divided into A type stainless steel substrates and Type B stainless steel substrates, and the A type stainless steel substrates can
It is different according to the diameter of circular hole, it is divided into 5 kinds, is respectively labeled as A1~A5；Base of the Type B stainless steel substrates in A type stainless steel substrates
On plinth, the central location of circular hole increases a rectangular workspace (20mm × 20mm), it may be assumed that stainless steel substrates center position is cut into one
The square hollow out of a 20mm × 20mm, corresponding glass panel region (20mm × 20mm) are added using scribing line etching method
Work, 20 μm of line width, line-spacing 2mm is divided into 100 small lattice, different according to the diameter of circular hole, is also divided into 5 kinds, be respectively labeled as B1~
B5。
The preparation method of the active somatic cell batch transfer blade the following steps are included:
1) stainless steel substrates are processed: choosing stainless steel substrates, selection has the laser cutting machine of computer numerical control device, and copy is cut
Data are cut to computer, carry out Laser Oxygen lancing, after the completion of cutting, deburring, notch of polishing；
In step 1), 316 stainless steel substrates that the stainless steel substrates can be used with a thickness of 1mm；The Laser Oxygen lancing
Germany can be used and lead to fast TruLaser 3030；Diamond file deburring can be used in the deburring；The polishing notch
400 mesh carborundum papers polishing notch can be used, the polishing of 2000 mesh sand paper can be used in the notch.
2) quartz glass plate is processed: quartz glass plate being imported cutting data to computer, is cut by laser；
In step 2), quartz glass plate that the quartz glass plate can be used with a thickness of 0.8mm；The laser cutting can
It is processed in the rectangular workspace (20mm × 20mm) of Type B stainless steel substrates using scribing line etching method, 20 μm of line width, line-spacing 2mm, respectively
For 100 small lattice.
3) seamless adhesion: quartz glass plate is lain against on light-transmitting plate, and A type stainless steel substrates are regardless of front and back sides, Type B stainless steel
The rectangular work ride of piece is placed up, after AB glue is mixed, AB glue is applied on stainless steel substrates, then vertical pile
It in quartz glass on piece, squeezing, alignment, overturning checks that there are bubble-free and gap in the back side, if there is bubble, stainless steel substrates are slided,
Squeeze bubble；If had the gap, stainless steel substrates and quartz glass plate are separated, again gluing；The bonding of Type B stainless steel substrates must be
It is operated under stereomicroscope or magnifying glass, is aligned stainless steel square hollow out with quartzy glass/glass piece scribe area, then seamless
Bonding；
In step 3), the mixing of 9940 heatproof AB glue of Lantian is can be used in the AB glue mixing.
4) modify: etc. after AB glues are fully cured, wipe the extra glue in side off, then remove circular hole and square workspace
Because squeezing the glue overflowed, powder is removed in stainless steel substrates of polishing, polishing, and active somatic cell batch transfer blade is made.
In step 4), it is described wipe the extra glue in side off cutter can be used wipe the extra glue in side off；The polishing
400 mesh carborundum papers polishing stainless steel substrates can be used in stainless steel substrates；The polishing of 2000 mesh sand paper can be used in the polishing；It is described clear
It is powdered away except powder can be used cotton swab and be stained with 70% alcohol wipe stainless steel substrates with removing, wherein the cleaning of Type B square workspace
It to be carried out under stereomicroscope or magnifying glass, the operation of blade tilt glass surface prevents plowing from glass surface and workspace
Scribing line.
Compared with prior art, the invention has the following outstanding advantages:
Active somatic cell batch transfer blade prepared by the present invention is substituted as conventional method-water droplet method tool, design
At the well (20,30,40,50,60 μ L) of different capabilities, as needed, the transfer blade of different capabilities can be selected.Nothing is added
To well, liquid will be evenly distributed to well, and form fixed liquid depth bacterium water, hence it is evident that it is bent to reduce liquid surface
The problems such as degree can effectively solve under microscope cell difficulty and position, easy to be lost in cell cleaning process, when Image Acquisition, can be maximum
Cell true form is presented in degree.Water evaporation rate is reduced simultaneously, gives the researcher longer operating time.
For Type B stainless steel substrates on the basis of A type stainless steel substrates, center increases a rectangular dashing work area.It is this to set
Meter operates under the microscope convenient for researcher, can not be needed frequently more while fast grabbing cell according to scribing line grid positioning
Cell separation frame and transfer blade are changed, to save time and efforts.Meanwhile Type B can also be used as the exclusive use of cell count frame.
The present invention chooses 316 stainless steel materials, and performance is stablized, and high temperature resistant is resistant to low concentration soda acid, seawater corrosion；Ultrawhite
High saturating quartz glass plate, translucency is good, compares with common glass slide, and will not change colour yellowing after the time is long, and considerably increasing makes
Use the service life；9940 heatproof AB glues, high temperature resistant, water-fast, oil resistant, acid and alkali-resistance and solvent and chemicals.Therefore the present invention can be whole
High pressure sterilization, to effectively reduce the external source germ contamination in use process.
In addition, the outer specification of the present invention is consistent with mainstream glass slide specification in the market, it is suitble to most of band grooves, card slot and pressure
The microscope of piece folder matches.
Detailed description of the invention
Fig. 1 is the structural schematic diagram of active somatic cell batch transfer blade embodiment of the present invention.
Fig. 2 is the high quartz glass plate schematic diagram thoroughly of ultrawhite.
Specific embodiment
Following embodiment will the present invention is further illustrated in conjunction with attached drawing.
Referring to Fig. 1 and 2, the active somatic cell batch transfer blade embodiment is equipped with stainless steel substrates and sheet glass, described stainless
Steel disc longitudinal stack bonds between stainless steel substrates and sheet glass on sheet glass.
The high saturating quartz glass plate of ultrawhite can be used in the stainless steel substrates for band 316 stainless steel substrates of circular hole, the sheet glass,
Heatproof AB glue seamless adhesion can be used in the bonding；The specification of the stainless steel substrates can be long 63.94mm, wide 25.26mm, thickness
1mm；The specification of the sheet glass can be long 75.90mm, wide 25.30mm, thick 0.8mm；LEAFTOP/ indigo plant can be used in the AB glue
9940 heatproof AB glue of field；The Circularhole diameter of the stainless steel substrates may respectively be 5,6,7,8,9mm；
Circular hole on the stainless steel substrates can be divided into A type stainless steel substrates and Type B stainless steel substrates, and the A type stainless steel substrates can
It is different according to the diameter of circular hole, it is divided into 5 kinds, is respectively labeled as A1~A5；Base of the Type B stainless steel substrates in A type stainless steel substrates
On plinth, the central location of circular hole increases a rectangular workspace (20mm × 20mm), it may be assumed that stainless steel substrates center position is cut into
The square hollow out of one 20mm × 20mm, corresponding glass panel region (20mm × 20mm), using scribing line etching method
Processing, 20 μm of line width, line-spacing 2mm is divided into 100 small lattice, different according to the diameter of circular hole, is also divided into 5 kinds, is respectively labeled as B1
~B5.
The preparation method of the active somatic cell batch transfer blade the following steps are included:
1) stainless steel substrates are processed: choosing stainless steel substrates, selection has the laser cutting machine of computer numerical control device, copy
Cutting data carries out Laser Oxygen lancing to computer, after the completion of cutting, deburring, and notch of polishing；The stainless steel substrates can be adopted
With 316 stainless steel substrates with a thickness of 1mm；The Laser Oxygen lancing can be used Germany and lead to fast TruLaser 3030；The removing
Diamond file deburring can be used in burr；400 mesh carborundum papers polishing notch can be used in the polishing notch, described to cut
The polishing of 2000 mesh sand paper can be used in mouth.
2) quartz glass plate is processed: quartz glass plate being imported cutting data to computer, is cut by laser；The quartz
The quartz glass plate that sheet glass can be used with a thickness of 0.8mm；The laser cutting can be in the rectangular workspace of Type B stainless steel substrates
(20mm × 20mm) using scribing line etching method processing, 20 μm of line width, line-spacing 2mm is divided into 100 small lattice.
3) seamless adhesion: quartz glass plate is lain against on light-transmitting plate, and A type stainless steel substrates are regardless of front and back sides, Type B stainless steel
The rectangular work ride of piece is placed up, after AB glue is mixed, AB glue is applied on stainless steel substrates, then vertical pile
It in quartz glass on piece, squeezing, alignment, overturning checks that there are bubble-free and gap in the back side, if there is bubble, stainless steel substrates are slided,
Squeeze bubble；If had the gap, stainless steel substrates and quartz glass plate are separated, again gluing；The bonding of Type B stainless steel substrates must be
It is operated under stereomicroscope or magnifying glass, is aligned stainless steel square hollow out with quartzy glass/glass piece scribe area, then seamless
Bonding；The mixing of 9940 heatproof AB glue of Lantian can be used in the AB glue mixing.
4) modify: etc. after AB glues are fully cured, wipe the extra glue in side off, then remove circular hole and square workspace
Because squeezing the glue overflowed, powder is removed in stainless steel substrates of polishing, polishing, and active somatic cell batch transfer blade is made.It is described to wipe side off
Extra glue can be used cutter and wipe the extra glue in side off；The polishing stainless steel substrates can be used 400 mesh carborundum papers and beat
Grind stainless steel substrates；The polishing of 2000 mesh sand paper can be used in the polishing；The removing powder can be used cotton swab and be stained with 70% alcohol wipe
Stainless steel substrates are powdered away with removing, wherein the cleaning of B money square workspace will under stereomicroscope or magnifying glass into
Row, blade tilt glass surface operation, prevents plowing from the scribing line of glass surface and workspace.
Test and examination of the invention is given below:
According to design drawing, the circular hole of diameter 5,6,7,8,9mm, in addition the thickness after AB glue bonding, circular hole capacity are respectively as follows:
20,30,40,50,60 μ L add water to test respectively with pipettor, and water does not spill over circular hole, do not flow into adjacent circular holes, gluing position without
Water stain, microscopically observation circular hole and Type B stainless steel substrates drawn area glass surface are not damaged, are wrapped up with gauze, 121 DEG C of high pressures are gone out
Bacterium 30min, stainless steel substrates and sheet glass still bond securely, as qualified.
Claims (10)
1. active somatic cell batch transfer blade, it is characterised in that be equipped with stainless steel substrates and sheet glass, the stainless steel substrates longitudinal stack
In on sheet glass, bonded between stainless steel substrates and sheet glass.
2. active somatic cell batch transfer blade as described in claim 1, it is characterised in that the stainless steel substrates are that band circular hole 316 is stainless
Steel disc, the sheet glass use quartz glass plate, and the bonding uses heatproof AB glue seamless adhesion；The specification of the stainless steel substrates
For long 63.94mm, wide 25.26mm, thick 1mm；The specification of the sheet glass is long 75.90mm, wide 25.30mm, thick 0.8mm；Institute
The Circularhole diameter for stating stainless steel substrates is respectively 5~9mm.
3. active somatic cell batch transfer blade as described in claim 1, it is characterised in that the circular hole on the stainless steel substrates is divided into A type
Stainless steel substrates and Type B stainless steel substrates, the A type stainless steel substrates are different according to the diameter of circular hole, are divided into 5 kinds, are respectively labeled as A1
~A5；On the basis of A type stainless steel substrates, the central location of circular hole increases a rectangular workspace and is the Type B stainless steel substrates
20mm × 20mm is 20mm × 20mm with corresponding glass panel region, is processed using scribing line etching method, 20 μm of line width, line-spacing
2mm is divided into 100 small lattice, different according to the diameter of circular hole, is also divided into 5 kinds, is respectively labeled as B1~B5.
4. the preparation method of active somatic cell batch transfer blade as described in claims 1 to 3, it is characterised in that the following steps are included:
1) stainless steel substrates are processed: choosing stainless steel substrates, selection has the laser cutting machine of computer numerical control device, copy cutting number
According to computer is arrived, Laser Oxygen lancing is carried out, after the completion of cutting, deburring, notch of polishing；
2) quartz glass plate is processed: quartz glass plate being imported cutting data to computer, is cut by laser；
3) seamless adhesion: quartz glass plate is lain against on light-transmitting plate, A type stainless steel substrates regardless of front and back sides, Type B stainless steel substrates
Rectangular work ride is placed up, after AB glue is mixed, AB glue is applied on stainless steel substrates, then vertical pile is in stone
It on English sheet glass, squeezes, alignment, overturning checks that there are bubble-free and gap in the back side, if there is bubble, slides stainless steel substrates, squeezes
Fall bubble；If had the gap, stainless steel substrates and quartz glass plate are separated, again gluing；The bonding of Type B stainless steel substrates must be stereoscopic
It is operated under microscope or magnifying glass, is aligned stainless steel square hollow out with quartzy glass/glass piece scribe area, then seamless adhesion；
4) it modifies: after AB glue is fully cured, wiping the extra glue in side off, then remove circular hole and square workspace is overflow because squeezing
Glue out, stainless steel substrates of polishing, polishing remove powder, active somatic cell batch transfer blade are made.
5. the preparation method of active somatic cell batch transfer blade as claimed in claim 4, it is characterised in that in step 1), it is described not
The steel disc that becomes rusty uses 316 stainless steel substrates with a thickness of 1mm；The Laser Oxygen lancing leads to fast TruLaser3030 using Germany.
6. the preparation method of active somatic cell batch transfer blade as claimed in claim 4, it is characterised in that described clear in step 1)
Flash removed uses diamond file deburring.
7. the preparation method of active somatic cell batch transfer blade as claimed in claim 4, it is characterised in that described to beat in step 1)
Notch is ground using 400 mesh carborundum papers polishing notch, the notch is polished using 2000 mesh sand paper.
8. the preparation method of active somatic cell batch transfer blade as claimed in claim 4, it is characterised in that in step 2), the stone
The quartz glass plate that English sheet glass uses with a thickness of 0.8mm；Rectangular workspace 20mm of the laser cutting in Type B stainless steel substrates
× 20mm is using scribing line etching method processing, and 20 μm of line width, line-spacing 2mm is divided into 100 small lattice.
9. the preparation method of active somatic cell batch transfer blade as claimed in claim 4, it is characterised in that in step 3), the AB
Glue is mixed to be mixed using 9940 heatproof AB glue of Lantian.
10. the preparation method of active somatic cell batch transfer blade as claimed in claim 4, it is characterised in that described in step 4)
It wipes the extra glue in side off and the extra glue in side is wiped off using cutter；The polishing stainless steel substrates use 400 mesh silicon carbide sands
Paper polishing stainless steel substrates；The polishing is polished using 2000 mesh sand paper；The removing powder is stained with 70% alcohol wipe using cotton swab
Stainless steel substrates are powdered away with removing, wherein the cleaning of Type B square workspace will under stereomicroscope or magnifying glass into
Row, blade tilt glass surface operation, prevents plowing from the scribing line of glass surface and workspace.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201811002454.7A CN109085696A (en) | 2018-08-30 | 2018-08-30 | Active somatic cell batch transfer blade and preparation method thereof |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201811002454.7A CN109085696A (en) | 2018-08-30 | 2018-08-30 | Active somatic cell batch transfer blade and preparation method thereof |
Publications (1)
| Publication Number | Publication Date |
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| CN109085696A true CN109085696A (en) | 2018-12-25 |
Family
ID=64795297
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
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| CN201811002454.7A Pending CN109085696A (en) | 2018-08-30 | 2018-08-30 | Active somatic cell batch transfer blade and preparation method thereof |
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| CN1046303A (en) * | 1990-05-03 | 1990-10-24 | 江西省机械工业设计研究院 | Technology for cutting hard alloy steel die with laser |
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| CN201051175Y (en) * | 2006-11-27 | 2008-04-23 | 上海市闵行中学 | Limit film and humidity keeping component for biologic medial organization slice |
| CN102775051A (en) * | 2012-06-13 | 2012-11-14 | 北京工业大学 | Glass cutting method |
| CN103627619A (en) * | 2012-08-23 | 2014-03-12 | 徐元诚 | Grid positioning sheet capable of detecting sperms quickly |
| CN205750100U (en) * | 2016-06-28 | 2016-11-30 | 中国人民解放军军事医学科学院附属医院 | Cell is cultivated and immunocytochemistry dyeing apparatus |
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2018
- 2018-08-30 CN CN201811002454.7A patent/CN109085696A/en active Pending
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1046303A (en) * | 1990-05-03 | 1990-10-24 | 江西省机械工业设计研究院 | Technology for cutting hard alloy steel die with laser |
| CN1204363A (en) * | 1995-10-06 | 1999-01-06 | 麦克罗克隆宁Cccd公司 | Compact cell culture slide |
| CN201051175Y (en) * | 2006-11-27 | 2008-04-23 | 上海市闵行中学 | Limit film and humidity keeping component for biologic medial organization slice |
| CN102775051A (en) * | 2012-06-13 | 2012-11-14 | 北京工业大学 | Glass cutting method |
| CN103627619A (en) * | 2012-08-23 | 2014-03-12 | 徐元诚 | Grid positioning sheet capable of detecting sperms quickly |
| CN205750100U (en) * | 2016-06-28 | 2016-11-30 | 中国人民解放军军事医学科学院附属医院 | Cell is cultivated and immunocytochemistry dyeing apparatus |
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Application publication date: 20181225 |