CN109077945A - Combined bacteriostat with lysozyme for cosmetics - Google Patents
Combined bacteriostat with lysozyme for cosmetics Download PDFInfo
- Publication number
- CN109077945A CN109077945A CN201810909401.7A CN201810909401A CN109077945A CN 109077945 A CN109077945 A CN 109077945A CN 201810909401 A CN201810909401 A CN 201810909401A CN 109077945 A CN109077945 A CN 109077945A
- Authority
- CN
- China
- Prior art keywords
- lysozyme
- cosmetics
- bacteriostat
- sensiva
- pentanediol
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
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Classifications
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/30—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds
- A61K8/64—Proteins; Peptides; Derivatives or degradation products thereof
- A61K8/65—Collagen; Gelatin; Keratin; Derivatives or degradation products thereof
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/30—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds
- A61K8/33—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds containing oxygen
- A61K8/34—Alcohols
- A61K8/345—Alcohols containing more than one hydroxy group
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/30—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds
- A61K8/33—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds containing oxygen
- A61K8/36—Carboxylic acids; Salts or anhydrides thereof
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
- A61Q17/00—Barrier preparations; Preparations brought into direct contact with the skin for affording protection against external influences, e.g. sunlight, X-rays or other harmful rays, corrosive materials, bacteria or insect stings
- A61Q17/005—Antimicrobial preparations
Abstract
The invention discloses a kind of Combined bacteriostat with lysozyme for cosmetics, it compound forming by lysozyme with Sensiva SC50 and decoyl hydroximic acid, pentanediol or hexylene glycol, water, the mass percent of each component are as follows: lysozyme 0.3%~2%, Sensiva SC50 0.1%~2%, decoyl hydroximic acid 0.1%~6%, pentanediol or hexylene glycol 8%~20%, water add to 100%.It is 5%~15% that Combined bacteriostat with lysozyme of the present invention, which is applied to the additive amount in Traditional makeup aqua, both common bacteria, fungi and saccharomycete therein can be effectively suppressed, guarantee the quality safety of cosmetics, simultaneously by synergistic function greatly reduce after single raw material largely add bring generate heat, have a fever, the irritations such as rubescent, have the characteristics that degradable green, noresidue and safe for traditional preservatives.
Description
Technical field
The invention belongs to cosmetic technical fields, and in particular to a kind of homology, safety is degradable, low stimulation, without sensitization
Property be used in the bacteriostatic agent for guaranteeing quality between cosmetics shelf and validity period in cosmetics.
Background technique
Currently, there are many disputes in antiseptic activity ingredient commonly used by cosmetics.The potential cause of formaldehyde and Form aldehyde release body
There are high irritation paraben esters and hormone secretions and certain for carcinous, organic halogen compound potential sensitization, Phenoxyethanol
It is relevant between particular cancers.And have researches show that: cook bacteriostatic agent using unconventional polyalcohols, it is effective antibacterial to reach
Effect, it is 3.2% for cosmetics gross mass that pentanediol least effective dose (LED), which is used alone, and it is minimum that hexylene glycol is used alone
Effective quantity is that mass ratio is 2.5%, however, found through practical studies, be added in cosmetics series 3% or more pentanediol or
1.5% or more hexylene glycol consumer has burning heat sensation in use.Effective ways there is no to solve the problems, such as this at present.Therefore
Cosmetics circle need to find some alternative low stimulations, without new bacteriostatic agent carcinogenic, degradable without sensitization, safety.
Lysozyme be current it has been found that the generally recognized as safe that can be degraded bacteriostatic agent, be usually used in the systems such as food, medicine
It is middle to be used as anti-corrosion addition, it can also be used to be used to the various odontopathies such as pre- anti-caries in dental care product.
Although lysozyme has good killing effect to part gram-positive bacteria, to normal in the cosmetics such as Escherichia coli
The inhibitory effects such as the Gram-negative bacteria seen are very poor, to fungies such as aspergillus niger, Candida albicans almost without inhibitory effect.Cause
For, from the mechanism of action for, lysozyme mainly reaches bacteriostasis, gram by the peptidoglycan layer of cell wall hydrolysis
The peptidoglycan layer of positive bacteria is located at the outermost layer of bacteria cell wall, and the peptidoglycan layer of Gram-negative bacteria is then located at bacterial cell
The inside of wall, external there are also epicytes, and the main component of fungal cell wall is chitin, different from bacterium.
Lysozyme multi-purpose greatly mainly solves certain due to gram sun in cosmetic formulations as active constituent use
Property microbial skin problem, not as guaranteeing what the bacteriostatic agent of cosmetics shelf quality safety used.Therefore, by bacteriolyze
Enzyme is applied to use in cosmetics as bacteriostatic agent, and by certain compounded technology raising lysozyme to Gram-negative bacteria with
And the research of fungi anti-microbial property has positive application value to the development of cosmetics.
Summary of the invention
The main object of the present invention is that traditional preservatives irritation is big, there are security risk and non-biographies in solution cosmetics
System bacteriostatic agent inhibitory effect is poor, be not sufficient to ensure that cosmetics shelf and use during stability the problems such as, a kind of application is provided
Gram-negative bacteria, gram-positive bacteria and yeast, aspergillus niger etc. can be effectively inhibited to make up in Traditional makeup aqua
The growth of strain is commonly used in product, it is ensured that the Combined bacteriostat with lysozyme of product quality.
For above-mentioned purpose, Combined bacteriostat with lysozyme of the invention is by lysozyme and Sensiva SC50 and decoyl hydroxyl
Oxime acid, pentanediol or hexylene glycol, water carry out compounding composition, and the mass percent of each component is as follows:
Above-mentioned Combined bacteriostat with lysozyme is preferably made of the raw material of following mass percents:
Above-mentioned Combined bacteriostat with lysozyme is also preferably made of the raw material of following mass percents:
Bacteriolyze enzyme source in the present invention can be plant origin, is also possible to animal origin or people recombinates lysozyme,
But the fungistatic effect that people recombinates lysozyme is better than other sources, and dosage in formula can reduce accordingly.
Sensiva SC50 in the present invention is the multifunctional cosmetics additive that whole world approval uses, while being also one kind
Effective deodorization ingredient, it has moisturizing and moisture-keeping function, and the surface tension by influencing microbial cell film, makes to press down
Microbial inoculum can more effectively penetrate into microbial cell, be remarkably improved the antimicrobial efficacy of traditional bacteriostatic agent；Decoyl hydroxyl
Oxime acid is that one kind can be used in cosmetics frequently as chelating agent, with the compound of chelated metal ions instead of traditional chelating
Agent such as EDTA etc., as what is used increases, the biocidal property of decoyl hydroximic acid is gradually well known, while the substance is to human body
Irritation is extremely low, fanout free region, is the excellent substitute of traditional preservatives.But that there is also antimicrobial spectrums is more single for decoyl hydroximic acid,
The disadvantages of fungistatic effect is weaker is used alone；Pentanediol is the moisturizer of a superior performance, frequently as moisturizing in cosmetics
Agent uses, and can have certain inhibition function to bacterium common in cosmetics when being added to a certain amount.
The present invention is answered Sensiva SC50, decoyl hydroximic acid, pentanediol or hexylene glycol and lysozyme based on lysozyme
Match, constitutes Combined bacteriostat with lysozyme, be greatly improved the inhibitory effect of lysozyme, reduce traditional preservatives in cosmetics
The security risks such as irritation and sensitization, while there are the spies such as degradable green, human body homology, highly-safe, noresidue
Point.
Combined bacteriostat with lysozyme of the invention has stronger fungistatic effect, matches applied to common cosmetics tradition aqua
Common bacteria, fungi and the saccharomycete in cosmetics can be effectively suppressed in side, and cosmetics are not easily susceptible in use
Secondary pollution both can satisfy the stable requirement of cosmetics quality, while it is excessive and cause to greatly reduce single component dosage
Rubescent, fever, irritative symptoms, the safety in utilization such as shouting pain it is higher.
Combined bacteriostat with lysozyme of the present invention using when be directly added to by 5%~15% dosage and be cooled to 45 DEG C or less
Aqua Material in stir evenly.
Specific embodiment
Below with reference to embodiment, the present invention is described in more detail, but protection scope of the present invention is not limited in these
Embodiment.
Embodiment 1
0.5g lysozyme, 0.3g Sensiva SC50,0.5g decoyl hydroximic acid, 20g pentanediol, 78.7g water is sufficiently mixed
Conjunction is uniformly dissolved, and obtains transparent Combined bacteriostat with lysozyme.
Embodiment 2
0.67g lysozyme, 0.33g Sensiva SC50,0.2g decoyl hydroximic acid, 18g pentanediol, 80.8g water is abundant
Mixed dissolution is uniform, obtains transparent Combined bacteriostat with lysozyme.
Embodiment 3
1g lysozyme, 0.2g Sensiva SC50,0.4g decoyl hydroximic acid, 10g pentanediol, 88.4g water are sufficiently mixed
It is uniformly dissolved, obtains transparent Combined bacteriostat with lysozyme.
Embodiment 4
2g lysozyme, 0.5g Sensiva SC50,0.1g decoyl hydroximic acid, 8g hexylene glycol, 92.4g water are sufficiently mixed molten
Solution uniformly, obtains transparent Combined bacteriostat with lysozyme.
Embodiment 5
0.8g lysozyme, 1g Sensiva SC50,1g decoyl hydroximic acid, 9g hexylene glycol, 89.2g water are sufficiently mixed dissolution
Uniformly, transparent Combined bacteriostat with lysozyme is obtained.
Embodiment 6
0.5g lysozyme, 0.1g Sensiva SC50,6g decoyl hydroximic acid, 10g pentanediol, 83.4g water are sufficiently mixed
It is uniformly dissolved, obtains transparent Combined bacteriostat with lysozyme.
In order to prove beneficial effects of the present invention, the Combined bacteriostat with lysozyme in embodiment 1 is used to prepare by inventor
In crease-resistant compact smoothing toner and anti-oxidant essence, wherein crease-resistant compact smoothing toner formula is shown in Table 1, anti-oxidant essence formula is shown in Table 2.
The crease-resistant compact smoothing toner formula of table 1
The anti-oxidant essence formula of table 2
Preservation challenge test is carried out to above-mentioned crease-resistant compact smoothing toner and anti-oxidant essence, the specific test method is as follows:
1, culture medium and other materials: nutrient agar, the red agar medium of tiger, 0.85% sodium chloride solution, training
Support ware, oese, incubator etc..
2, by microorganism on probation: test is provided with bacterium and fungi by foods in Shaanxi province and institute for drug control, bacterium packet
It includes: escherichia coli (Escherichia Coli, ATCC 8739), pseudomonas aeruginosa (Pseudomonas
Aeruginosa, ATCC 9027), staphylococcus aureus (Staphylococcus Aureus, ATCC 6538), onion primary
Ke Huoerde Salmonella (Burkholderia cepacia, BC, ATCC 25416), pseudomonas putida (Pseudomonas
Putida, ATCC 17485), fungi includes: Candida albicans (Candida Albicans, ATCC 10231), aspergillus niger
(Aspergillus Niger, ATCC 16404).
3, microculture: before experiment, each strain being inoculated in suitable culture medium, in 37 DEG C (bacteriums) or 28 DEG C
(fungi) culture.After 2 days, fungal culture selected appropriate bacterium colony in the physiological saline of sterilizing after 3~5 days to Bacteria Culture,
Certain density mixed cell suspension (3 × 10 is made8A/mL) or mixed fungus suspension (1 × 107A/mL), it is placed in 4 DEG C of storages
It is spare.
4, once add 28 days Microbial Challenges test of bacterium
Each given the test agent 30g is weighed, mixed cell suspension or mixed fungus suspension is added, every gram is finally contained bacterium by sample product
Amount about 106A bacterium and 105A fungi.Then it mixes well, is placed at 28 DEG C.And respectively at connecing 0,7,14,21 and the 28 of bacterium
Its sampling analysis: accurately weighing 3g sample, is added in the sterilizing conical flask containing glass bead, and 27mL sterile physiological salt is added
Mixing fullys shake in water, this suspension is 1:10 dilution；Then it is successively diluted with sterile saline by 10 times again.By plate
Tilt-pour process is counted by bacteria containing amount in test product, Bacteria Culture condition: 37 DEG C, 1~2 day, fungal culture condition: 28 DEG C, 3~5 days.Point
Analysis criterion is shown in Table 3, and analysis the results are shown in Table 4 and table 5.
3 analysis rule of table
The challenge experimental result of the crease-resistant compact smoothing toner of table 4
The challenge experimental result of the anti-oxidant essence of table 5
From table 4 and the test result of table 5 as it can be seen that the crease-resistant compact smoothing toner of sample and anti-oxidant essence the 7th after connecing bacterium,
14, there is bacterium without detection within 21,28 days, lose growth in 28 days, tested by preservation challenge, show lysozyme of the invention
Combined bacteriostat has stronger bacteriostasis property.
Above-mentioned crease-resistant compact smoothing toner and anti-oxidant essence product are through testing, and without any burning heat sensation, biocidal property is stronger, product
It is not easily susceptible to secondary pollution in storage and use process, physical and chemical performance index meets related quality criterion requirement.
Claims (3)
1. a kind of Combined bacteriostat with lysozyme for cosmetics, it is characterised in that the bacteriostatic agent by following mass percents original
Material composition:
2. Combined bacteriostat with lysozyme according to claim 1, it is characterised in that the bacteriostatic agent is by following mass percents
Raw material composition:
3. Combined bacteriostat with lysozyme according to claim 1, it is characterised in that the bacteriostatic agent is by following mass percents
Raw material composition:
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201810909401.7A CN109077945A (en) | 2018-08-10 | 2018-08-10 | Combined bacteriostat with lysozyme for cosmetics |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201810909401.7A CN109077945A (en) | 2018-08-10 | 2018-08-10 | Combined bacteriostat with lysozyme for cosmetics |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN109077945A true CN109077945A (en) | 2018-12-25 |
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ID=64834175
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201810909401.7A Pending CN109077945A (en) | 2018-08-10 | 2018-08-10 | Combined bacteriostat with lysozyme for cosmetics |
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| Country | Link |
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| CN (1) | CN109077945A (en) |
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110840765A (en) * | 2019-12-12 | 2020-02-28 | 陕西省石油化工研究设计院 | Antiseptic and bactericidal composition and preparation method thereof |
| CN111346015A (en) * | 2020-03-12 | 2020-06-30 | 广州艾卓生物科技有限公司 | Cosmetic composition and method for preparing the same |
| CN111358742A (en) * | 2020-05-15 | 2020-07-03 | 广州比力福生物科技有限公司 | Compound plant anti-hair loss and hair growth liquid |
| CN111529463A (en) * | 2020-05-15 | 2020-08-14 | 广州比力福生物科技有限公司 | Compound plant anti-allergy agent |
Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1679504A (en) * | 2005-01-26 | 2005-10-12 | 安米 | Use of human lysozyme in cosmetics for treating acne |
| CN105997545A (en) * | 2016-06-30 | 2016-10-12 | 广州艾卓生物科技有限公司 | Compound preservative containing glycerol caprylate and decoyl hydroximic acid and preparation method of c ompound preservative |
| CN106726658A (en) * | 2017-03-13 | 2017-05-31 | 福州美乐莲生物科技有限公司 | Preservative of a kind of hydroximic acid containing decoyl and parahydroxyacet-ophenone and its preparation method and application and the cosmetics containing the preservative |
| CN107432834A (en) * | 2017-07-19 | 2017-12-05 | 广州市皓雨化妆品有限公司 | It is a kind of with fungistatic effect without anti-corrosion composition composition and its preparation method and application |
| CN107550745A (en) * | 2017-08-31 | 2018-01-09 | 北京明弘科贸有限责任公司 | A kind of anticorrosive composite and its application |
-
2018
- 2018-08-10 CN CN201810909401.7A patent/CN109077945A/en active Pending
Patent Citations (5)
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|---|---|---|---|---|
| CN1679504A (en) * | 2005-01-26 | 2005-10-12 | 安米 | Use of human lysozyme in cosmetics for treating acne |
| CN105997545A (en) * | 2016-06-30 | 2016-10-12 | 广州艾卓生物科技有限公司 | Compound preservative containing glycerol caprylate and decoyl hydroximic acid and preparation method of c ompound preservative |
| CN106726658A (en) * | 2017-03-13 | 2017-05-31 | 福州美乐莲生物科技有限公司 | Preservative of a kind of hydroximic acid containing decoyl and parahydroxyacet-ophenone and its preparation method and application and the cosmetics containing the preservative |
| CN107432834A (en) * | 2017-07-19 | 2017-12-05 | 广州市皓雨化妆品有限公司 | It is a kind of with fungistatic effect without anti-corrosion composition composition and its preparation method and application |
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Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110840765A (en) * | 2019-12-12 | 2020-02-28 | 陕西省石油化工研究设计院 | Antiseptic and bactericidal composition and preparation method thereof |
| CN111346015A (en) * | 2020-03-12 | 2020-06-30 | 广州艾卓生物科技有限公司 | Cosmetic composition and method for preparing the same |
| CN111358742A (en) * | 2020-05-15 | 2020-07-03 | 广州比力福生物科技有限公司 | Compound plant anti-hair loss and hair growth liquid |
| CN111529463A (en) * | 2020-05-15 | 2020-08-14 | 广州比力福生物科技有限公司 | Compound plant anti-allergy agent |
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Application publication date: 20181225 |
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