CN109055262A - A kind of complex micro organism fungicide and preparation method thereof for feces of livestock and poultry low temperature compost fermentation - Google Patents
A kind of complex micro organism fungicide and preparation method thereof for feces of livestock and poultry low temperature compost fermentation Download PDFInfo
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- CN109055262A CN109055262A CN201810907570.7A CN201810907570A CN109055262A CN 109055262 A CN109055262 A CN 109055262A CN 201810907570 A CN201810907570 A CN 201810907570A CN 109055262 A CN109055262 A CN 109055262A
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/20—Bacteria; Culture media therefor
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05F—ORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C, e.g. FERTILISERS FROM WASTE OR REFUSE
- C05F17/00—Preparation of fertilisers characterised by biological or biochemical treatment steps, e.g. composting or fermentation
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05F—ORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C, e.g. FERTILISERS FROM WASTE OR REFUSE
- C05F3/00—Fertilisers from human or animal excrements, e.g. manure
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/14—Fungi; Culture media therefor
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
- Y02A40/10—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in agriculture
- Y02A40/20—Fertilizers of biological origin, e.g. guano or fertilizers made from animal corpses
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02W—CLIMATE CHANGE MITIGATION TECHNOLOGIES RELATED TO WASTEWATER TREATMENT OR WASTE MANAGEMENT
- Y02W30/00—Technologies for solid waste management
- Y02W30/40—Bio-organic fraction processing; Production of fertilisers from the organic fraction of waste or refuse
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Abstract
The present invention is suitable for microbial bacterial agent technical field, a kind of complex micro organism fungicide and preparation method thereof for feces of livestock and poultry low temperature compost fermentation is provided, obtained complex micro organism fungicide is pulvis microbial inoculum, packing cost is low, it is easy transport, bacterial content height can save at normal temperature and not easy in inactivation, when microbial inoculum is added in feces of livestock and poultry and straw compost material at low ambient temperatures, pyschrophile can quickly be bred using organic wastes such as stalk or excrement, generating biological heat makes material play temperature rapidly, high temperature is held time length, and it is accompanied by secondary temperature elevation, the scale livestock or poultry field of waste production organic fertilizer is handled under severe cold winter conditions especially suitable for northern area.
Description
Technical field
The invention belongs to microbial bacterial agent technical field more particularly to a kind of answering for feces of livestock and poultry low temperature compost fermentation
Close microbial bacterial agent and preparation method thereof.
Background technique
The yield of China's agricultural organic waste is huge, it is estimated that annual to generate 3,000,000,000 tons of feces of livestock and poultry, crops straw
More than 700,000,000 tons of stalk, nutrient needed for these organic wastes are rich in a large amount of crop productions.It can be with by the method for aerobic compost
It is high-quality organic fertilizer by these reclaiming organic wastes, Traditional compost method is needed since initial stage effective microbe quantity is few
Certain time could breed, and it is long that there are fermentation times, and foul smell discharge is big, and nutrient element loss is more, serious waste of resources etc.
Problem, especially northerly autumn and winter, since ambient temperature is low, it is especially prominent that compost fermentation plays the difficult problem of temperature.
Summary of the invention
In view of the above problems, the purpose of the present invention is to provide a kind of for the compound micro- of feces of livestock and poultry low temperature compost fermentation
Bacteria agent and preparation method thereof, it is intended to solve the existing complex micro organism fungicide gas for feces of livestock and poultry low temperature compost fermentation
Warm technical problem slow, temperature holding time is short.
A kind of preparation method of the complex micro organism fungicide for feces of livestock and poultry low temperature compost fermentation, the method includes under
State step:
A, strain culturing: by bacillus subtilis, bacillus amyloliquefaciens, Trichoderma viride, trichodermaharzianum and geotrichum candidum point
It is not inoculated into respective culture medium and is cultivated;
B, the preparation of microbial bacterial agent: every kind of bacterial strain that step a is cultivated is respectively with inclined plane inoculating to equipped with 200ml seed
It is individually cultivated in the 500ml medicine bottle of culture medium using fluid nutrient medium, obtains the bacterium solution of bacterial strain, then by bacterium solution according to withered grass gemma
Bacillus: bacillus amyloliquefaciens: Trichoderma viride: trichodermaharzianum: 1L seed is made in the mixing of geotrichum candidum=4:2:1:2:1 ratio
Liquid, then by seed liquor all access equipped with 200L fermentation medium 500L seeding tank in culture for the first fermentation, one
Inoculation obtains composite microbial bacteria equipped with second of fermented and cultured is carried out in 5 tons of seeding tanks of 3 tons of fermentation mediums after the section time
Liquid;
C, finished product makes: by the complex microorganism bacterium solution of 5 tons of seeding tank kind cultures after detection viable count is qualified, with micro- life
Object microbial inoculum spray duster drying tower, which is dried, to dust to get to the complex microorganism that can be used for feces of livestock and poultry low temperature compost fermentation
Microbial inoculum.
Specifically, bacillus subtilis uses LB agar medium in step a, is cultivated under the conditions of 30 DEG C for 24 hours, solve starch
Bacillus uses MBS agar medium, and 6h is cultivated under the conditions of 26 DEG C, and Trichoderma viride, trichodermaharzianum and geotrichum candidum use YPD
Agar medium cultivates 20h under the conditions of 18 DEG C respectively.
Specifically, bacillus subtilis uses LB liquid medium in step b, 48h is cultivated under the conditions of 30 DEG C, solves starch
Bacillus uses MBS fluid nutrient medium, and 12h is cultivated under the conditions of 26 DEG C, and Trichoderma viride, trichodermaharzianum and geotrichum candidum use
YPD fluid nutrient medium, cultivates 40h under the conditions of 18 DEG C, and the cultivation temperature of first time fermented and cultured is that 16 DEG C of incubation times are
40h, the cultivation temperature of second of fermented and cultured are that 10 DEG C of incubation times are 60h.
Specifically, fermentation medium components described in step b are as follows: glucose 10g, mealy potato 50g, peptone 5g, phosphoric acid
Monoammonium 8g, potassium nitrate 2g, calcium carbonate 0.5g, pure water 1000ml, pH 6.8 are sterilized under conditions of 125 DEG C using preceding
30min。
It is described to be used for livestock and poultry the present invention also provides a kind of complex micro organism fungicide for feces of livestock and poultry low temperature compost fermentation
The complex micro organism fungicide of excrement low temperature compost fermentation is by a kind of composite microbial for feces of livestock and poultry low temperature compost fermentation
The preparation method of object microbial inoculum is prepared.
A kind of complex micro organism fungicide and preparation method thereof for feces of livestock and poultry low temperature compost fermentation provided by the invention
Step is simple, and complex micro organism fungicide is pulvis microbial inoculum, and packing cost is low, is easy transport, and bacterial content height can be protected at normal temperature
It deposits and not easy in inactivation, when microbial inoculum is added in feces of livestock and poultry and straw compost material at low ambient temperatures, pyschrophile can be utilized
The organic wastes such as stalk or excrement are quickly bred, and generating biological heat makes material play temperature rapidly, are carried out hot fermentation and are removed
It is smelly, low-temperature limit is breached, 0 DEG C or less high temperature Rapid Fermentation is realized.
Specific embodiment
In order to make the objectives, technical solutions, and advantages of the present invention clearer, with reference to embodiments, to the present invention
It is further elaborated.It should be appreciated that the specific embodiments described herein are merely illustrative of the present invention, it is not used to
Limit the present invention.
Embodiment 1:
A kind of preparation method of the complex micro organism fungicide for feces of livestock and poultry low temperature compost fermentation, the method includes under
State step:
A, strain culturing: by bacillus subtilis, bacillus amyloliquefaciens, Trichoderma viride, trichodermaharzianum and geotrichum candidum point
It is not inoculated into respective culture medium and is cultivated, wherein bacillus subtilis is used LB agar medium, 30 DEG C of cultures
For 24 hours, bacillus amyloliquefaciens use MBS agar medium, 26 DEG C of culture 6h, and Trichoderma viride, trichodermaharzianum and geotrichum candidum use
YPD Agr culture medium, 18 DEG C of culture 20h.
B, the preparation of microbial bacterial agent: every kind of bacterial strain that step a is cultivated is respectively with inclined plane inoculating to equipped with 200ml seed
It is individually cultivated in the 500ml medicine bottle of culture medium using fluid nutrient medium, wherein bacillus subtilis uses LB liquid medium,
48h is cultivated under the conditions of 30 DEG C, bacillus amyloliquefaciens use MBS fluid nutrient medium, and 12h, green wood are cultivated under the conditions of 26 DEG C
Mould, trichodermaharzianum and geotrichum candidum use YPD fluid nutrient medium, cultivate 40h under the conditions of 18 DEG C, obtain the bacterium solution of bacterial strain, then will
Bacterium solution is according to bacillus subtilis: bacillus amyloliquefaciens: Trichoderma viride: trichodermaharzianum: geotrichum candidum=4:2:1:2:1 ratio
Mixing, is made 1L seed liquor, and then by seed liquor, all access is equipped in the 500L seeding tank of 200L fermentation medium at 16 DEG C
Under the conditions of culture 40h for the first fermentation, after a period of time inoculation equipped with 3 tons of fermentation mediums 5 tons of seeding tanks in 10
Second of fermented and cultured 60h is carried out under the conditions of DEG C, obtains complex microorganism bacterium solution.Wherein fermentation medium components are as follows: glucose
10g, mealy potato 50g, peptone 5g, monoammonium phosphate 8g, potassium nitrate 2g, calcium carbonate 0.5g, pure water 1000ml, pH 6.8 make
With the preceding 30min that sterilizes under conditions of 125 DEG C.
C, finished product makes: by the complex microorganism bacterium solution of 5 tons of seeding tank kind cultures after detection viable count is qualified, with micro- life
Object microbial inoculum spray duster drying tower, which is dried, to dust to get to the complex microorganism that can be used for feces of livestock and poultry low temperature compost fermentation
Microbial inoculum.
The complex micro organism fungicide for being used for feces of livestock and poultry low temperature compost fermentation that embodiment is obtained is being not less than -25 DEG C
Under temperature condition, and the animal wastes of cow dung, chicken manure etc. are mixed according to the ratio of 1~2:500~600, utilize such as farming
The auxiliary materials such as object stalk, gardens pruning object adjust 20~30:1 of mixed material carbon-nitrogen ratio, then moisture content 55~60% is covered using film
Lid compost fermentation carries out Composting treatment, and composting process guarantees that oxygen concentration is 15% or more, achieves that livestock and poultry within 15 days or so
Excrement is completely decomposed, produces high-quality organic fertilizer.
A kind of complex micro organism fungicide and preparation method thereof for feces of livestock and poultry low temperature compost fermentation provided by the invention
Under -25 DEG C of temperature condition, it so that compost material temperature is reached 30 DEG C or more within 2 days, achieve that livestock and poultry within 15 days or so
Excrement is completely decomposed, produces high-quality organic fertilizer.Complex micro organism fungicide of the invention has in refrigerant latitudes extreme severe cold
Winter conditions under ferment quick heating, high temperature is held time length, and is accompanied by secondary temperature elevation, especially suitable for northern area tight
The scale livestock or poultry field of waste production organic fertilizer is handled under the conditions of season in severe winter.
The foregoing is merely illustrative of the preferred embodiments of the present invention, is not intended to limit the invention, all in essence of the invention
Made any modifications, equivalent replacements, and improvements etc., should all be included in the protection scope of the present invention within mind and principle.
Claims (5)
1. a kind of preparation method of the complex micro organism fungicide for feces of livestock and poultry low temperature compost fermentation, which is characterized in that described
Method includes the following steps:
A, strain culturing: bacillus subtilis, bacillus amyloliquefaciens, Trichoderma viride, trichodermaharzianum and geotrichum candidum are connect respectively
Kind is cultivated into respective culture medium;
B, the preparation of microbial bacterial agent: every kind of bacterial strain that step a is cultivated is respectively with inclined plane inoculating to equipped with 200ml seed culture
It is individually cultivated in the 500ml medicine bottle of base using fluid nutrient medium, obtains the bacterium solution of bacterial strain, then by bacterium solution according to bacillus subtilis
Bacterium: bacillus amyloliquefaciens: Trichoderma viride: trichodermaharzianum: 1L seed liquor is made in the mixing of geotrichum candidum=4:2:1:2:1 ratio,
Then by seed liquor, all access is equipped with culture for the first fermentation in the 500L seeding tank of 200L fermentation medium, at one section
Between after inoculation equipped with 3 tons of fermentation mediums 5 tons of seeding tanks in carry out second of fermented and cultured, obtain complex microorganism bacterium solution;
C, finished product makes: by the complex microorganism bacterium solution of 5 tons of seeding tank kind cultures after detection viable count is qualified, using microbial bacteria
Agent spray duster drying tower, which is dried, to dust to get to the composite microbial bacteria that can be used for feces of livestock and poultry low temperature compost fermentation
Agent.
2. a kind of preparation method of the complex micro organism fungicide for feces of livestock and poultry low temperature compost fermentation as described in claim 1,
It is characterized in that, bacillus subtilis uses LB agar medium in step a, is cultivated under the conditions of 30 DEG C for 24 hours, solve starch gemma
Bacillus uses MBS agar medium, and 6h is cultivated under the conditions of 26 DEG C, and Trichoderma viride, trichodermaharzianum and geotrichum candidum use YPD Agr
Culture medium cultivates 20h under the conditions of 18 DEG C respectively.
3. a kind of preparation method of the complex micro organism fungicide for feces of livestock and poultry low temperature compost fermentation as described in claim 1,
It is characterized in that, bacillus subtilis uses LB liquid medium in step b, 48h is cultivated under the conditions of 30 DEG C, solves starch gemma
Bacillus uses MBS fluid nutrient medium, and 12h is cultivated under the conditions of 26 DEG C, and Trichoderma viride, trichodermaharzianum and geotrichum candidum use YPD liquid
Body culture medium, cultivates 40h under the conditions of 18 DEG C, and it is 40h that the cultivation temperature of first time fermented and cultured, which is 16 DEG C of incubation times, and second
The cultivation temperature of secondary fermented and cultured is that 10 DEG C of incubation times are 60h.
4. a kind of preparation method of the complex micro organism fungicide for feces of livestock and poultry low temperature compost fermentation as described in claim 1,
It is characterized in that, fermentation medium components described in step b are as follows: glucose 10g, mealy potato 50g, peptone 5g, monoammonium phosphate
8g, potassium nitrate 2g, calcium carbonate 0.5g, pure water 1000ml, pH 6.8 use the preceding 30min that sterilizes under conditions of 125 DEG C.
5. a kind of complex micro organism fungicide for feces of livestock and poultry low temperature compost fermentation, which is characterized in that described to be used for animal dung
Just the complex micro organism fungicide of low temperature compost fermentation is prepared by any one of claim 1-4 the method.
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Cited By (4)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN110156498A (en) * | 2019-04-30 | 2019-08-23 | 江苏思威博生物科技有限公司 | A kind of feces of livestock and poultry high temperature desiccation microbial inoculum and its application method |
CN110205354A (en) * | 2019-06-24 | 2019-09-06 | 黑龙江奥创生物科技发展有限公司 | A kind of preparation method of earthworm bioactivity fulvic acid |
CN113174386A (en) * | 2021-04-27 | 2021-07-27 | 新至农业生态科技有限公司 | Composite fermentation inoculant for manure composting |
CN115181706A (en) * | 2022-08-18 | 2022-10-14 | 山东省农业技术推广中心(山东省农业农村发展研究中心) | Microbial agent for livestock and poultry manure compost and preparation method thereof |
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CN103898032A (en) * | 2014-04-25 | 2014-07-02 | 江苏联海生物科技有限公司 | Complex microbial inoculant and method for preparing organic fertilizer by using same |
CN106399193A (en) * | 2016-10-26 | 2017-02-15 | 北京大伟嘉生物技术股份有限公司 | Microbial inoculants used for low temperature fermentation of organic fertilizers, and preparation method and application thereof |
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Cited By (6)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN110156498A (en) * | 2019-04-30 | 2019-08-23 | 江苏思威博生物科技有限公司 | A kind of feces of livestock and poultry high temperature desiccation microbial inoculum and its application method |
CN110205354A (en) * | 2019-06-24 | 2019-09-06 | 黑龙江奥创生物科技发展有限公司 | A kind of preparation method of earthworm bioactivity fulvic acid |
CN110205354B (en) * | 2019-06-24 | 2023-06-06 | 黑龙江奥创生物科技发展有限公司 | Preparation method of earthworm bioactive fulvic acid |
CN113174386A (en) * | 2021-04-27 | 2021-07-27 | 新至农业生态科技有限公司 | Composite fermentation inoculant for manure composting |
CN113174386B (en) * | 2021-04-27 | 2022-06-24 | 新至农业生态科技有限公司 | Composite fermentation microbial inoculum for manure compost |
CN115181706A (en) * | 2022-08-18 | 2022-10-14 | 山东省农业技术推广中心(山东省农业农村发展研究中心) | Microbial agent for livestock and poultry manure compost and preparation method thereof |
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Application publication date: 20181221 |