CN109043307A - A kind of semisolid enzymatic hydrolysis grain dust and its production method - Google Patents

A kind of semisolid enzymatic hydrolysis grain dust and its production method Download PDF

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Publication number
CN109043307A
CN109043307A CN201810663113.8A CN201810663113A CN109043307A CN 109043307 A CN109043307 A CN 109043307A CN 201810663113 A CN201810663113 A CN 201810663113A CN 109043307 A CN109043307 A CN 109043307A
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enzymatic hydrolysis
grain dust
production method
enzyme
enzyme preparation
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CN201810663113.8A
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Inventor
刘宇
林伟静
刘佳
李梦黎
赵菁菁
陈颖
周至富
康永兴
袁红旭
彭玉周
沈思佳
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BEIJING XIETONG INNOVATION FOOD TECHNOLOGY Co.,Ltd.
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Beijing Collaborative Innovation Institute
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Priority to CN201810663113.8A priority Critical patent/CN109043307A/en
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    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23LFOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
    • A23L7/00Cereal-derived products; Malt products; Preparation or treatment thereof
    • A23L7/10Cereal-derived products
    • A23L7/198Dry unshaped finely divided cereal products, not provided for in groups A23L7/117 - A23L7/196 and A23L29/00, e.g. meal, flour, powder, dried cereal creams or extracts
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23LFOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
    • A23L7/00Cereal-derived products; Malt products; Preparation or treatment thereof
    • A23L7/10Cereal-derived products
    • A23L7/104Fermentation of farinaceous cereal or cereal material; Addition of enzymes or microorganisms
    • A23L7/107Addition or treatment with enzymes not combined with fermentation with microorganisms

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  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Health & Medical Sciences (AREA)
  • Nutrition Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Food Science & Technology (AREA)
  • Polymers & Plastics (AREA)
  • Coloring Foods And Improving Nutritive Qualities (AREA)
  • Cereal-Derived Products (AREA)

Abstract

The present invention relates to a kind of semisolid enzymatic hydrolysis grain dust and its production methods, the present invention is mixed grain dust with enzyme preparation under the Water Content Conditions of 30-55% using the technique of semisolid enzymatic hydrolysis, enzymatic hydrolysis reaction is carried out at a certain temperature, can produce the grain dust containing polypeptide.It avoids using more complicated drying programs such as spray drying or roller dryings, significantly reduces power consumption of polymer processing and processing cost, be conducive to large-scale industrial production.

Description

A kind of semisolid enzymatic hydrolysis grain dust and its production method
Technical field
The invention belongs to technical field of agricultural product process, especially grain dust product processing technique field.
Background technique
Corn gluten protein hydrolysis generate polypeptide, have it is anti-oxidant, blood pressure lowering and promote fat metabolism, reduce cholesterol, Antifatigue and adjust the functions such as immune, the functional nutrient that can greatly improve cereal is horizontal, and more high added value has been provided in creation Product.Currently used enzymolysis process is carried out in liquid cereal slurry material, and process is various, enzymolysis process time-consuming compared with Long, subsequent enzyme deactivation needs higher energy input;Cereal needs complex using spray drying or roller drying etc. after enzymatic hydrolysis Drying program so that processing cost is higher, energy consumption is high, is unfavorable for large-scale industrial production.
Current existing cereal enzymolysis scheme is carried out using liquid enzyme solution.Traditional enzymolysis process is liquid Enzymatic isolation method, using large-size reactor, batch production, i.e. one pot one pot of production.Water content is very high when enzymatic hydrolysis, 70-90% with On.There is no Ultramicro Dispersion complete sets of Techniques, filtering technique therefore, after enzymatic hydrolysis is taken to filter off cellulose components.Then, thickening. Finally using spray drying or roller drying, enzymatic hydrolysis grain dust is made.There is process flow complexity, workshops for cylinder drying The problems such as personnel are more, and equipment is more, take up a large area, water, electricity, higher vapour consumption.Liquid process process energy consumption are as follows: agitating and heating section 100 degree/t, it is spray-dried 2500 degree/t of section, adds up to 2600 degree/t, energy consumption is huge, and increased costs are very high, reaches per kilogram 8-10 member or so.Simultaneously according to the technique, production main process is wet production process, and roller drying production line has huge work In air, Environmental microorganism control requires height to the exposure of industry face, and workshop needs to fit up and run in strict accordance with GMP, and investment is huge; And production line flow process is long, and equipment is huge, compares suitable for producing single variety, it is relatively difficult that different grain varieties change production.Roller Dry processing technology also causes grain dry temperature low, and the fragrance of product is very weak.The prior art uses liquid enzymolysis process, raw Production cost is very high, and product is difficult to be applied as ordinary food ingredient.In addition, the cereal produced using liquid enzymolysis process Powder has serious bitter taste, it is difficult to carry out food applications, greatly limit marketing due to the generation of bitter peptides.
Summary of the invention
In order to solve the above technical problems, the present invention provides a kind of semisolid enzymatic hydrolysis grain dust and its production method, adopt So that raw material is in semisolid, the i.e. state of dough in enzymolysis process with the technique that semisolid digests, by enzyme preparation and The control of technique can reach higher polypeptide yield, and production cost is greatly lowered, and improve product special flavour.
Technical solution of the present invention is as follows:
A kind of production method of semisolid enzymatic hydrolysis grain dust, comprising: enzyme preparation, water and grain dust are uniformly mixed, Dough state is made, makes the water content 30%-55% of material after mixing;It is digested under enzymatic hydrolysis condition appropriate;Alternatively, It further include that little particle state is made in the material after enzymatic hydrolysis, enzyme deactivation dry, pulverize.
Further, the enzyme preparation includes protease, specifically includes papain, bacterialprotease, carboxypeptidase, pancreas At least one of protease, beta lipoprotein enzyme etc..
Further, the dosage of the enzyme preparation (referring mainly to protease) is the 0.1-2% of grain dust weight, preferably 0.5-1%.
Specifically, commercialized enzyme preparation, such as Novozymes Company Alcalase (alkali protease), Novi's letter can be selected One or more of company Neutrase (neutral proteinase) etc..Preferably, the dosage of Alcalase is grain dust weight 0.2-2%, more preferably 0.5%;And/or, it is preferable that the dosage of Neutrase is the 0.1-2% of grain dust weight, more preferably It is 0.25%.
Further, appropriate sodium bicarbonate can also be added during dough is made or in enzymolysis process, preferably to promote Into the progress of enzymolysis process.Its dosage can be determined according to used enzyme preparation and/or cereal materials used, generally, little Su The dosage beaten can be the 0.1-2%, preferably 0.5-1.5% of grain dust weight.
Specifically, it after can first mixing enzyme preparation, sodium bicarbonate with water, adds in grain dust.
Preferably to improve hydrolysis result, grain dust can be crushed to 60-300 mesh, preferably 100-200 mesh.
Further, the cereal materials include one or more of oatmeal, red bean powder, buckwheat, soy meal etc..
Further, the water content 40%-55% of material after mixing can be made.
Enzymatic hydrolysis condition can be determined according to used enzyme preparation and cereal materials used, under normal circumstances can be in temperature 60- Under the conditions of 65 DEG C, 0.5-4h is digested.
Further, the material after enzymatic hydrolysis can be made to the little particle state of partial size 0.5-5mm, in order to subsequent step Rapid processing.
Enzyme deactivation condition can also be determined according to used enzyme preparation and/or cereal materials used, under normal circumstances can be in temperature Under the conditions of 100-120 DEG C of degree, 5-20min is digested.
Drying condition can also be determined according to used enzyme preparation and/or cereal materials used, under normal circumstances can be in temperature Dry 10-60min under the conditions of 60-100 DEG C of degree.
It can also be as needed by the crushing material after drying to required partial size.
Further, can before enzymatic hydrolysis terminates certain time (such as 20-40min, preferably 30min or so) be supplemented centainly The dried grains powder of (such as 5%-30% weight) is measured, is uniformly mixed, continues to digest;Material texture hardness can be made to improve, it is easier to Little particle state is made to be more easily fabricated into molding.
Specifically, the production method of above-mentioned semisolid enzymatic hydrolysis grain dust, comprising: mix enzyme preparation, sodium bicarbonate, water Uniformly, it is added in the grain dust of 60-300 mesh, is uniformly mixed, dough state is made, make the water content 30%- of material after mixing 55%;It is digested under enzymatic hydrolysis condition appropriate;20-40min is supplemented the dried grains of 5%-30% weight before enzymatic hydrolysis terminates Powder is uniformly mixed;Continue to digest;Material after enzymatic hydrolysis is made to the little particle state of partial size 0.5-5mm, enzyme deactivation is dry, It crushes.
The invention also includes the hydrolyzed cereal powder of the method preparation.Its content of peptides is up to 5-15%, proteolysis ratio Example is up to 50-65%.
The raw materials used in the present invention is commercially available to buy, or prepares by conventional method in that art.
On the basis of common knowledge of the art, above-mentioned each optimum condition can be combined with each other each preferably to get the present invention Example.
The present invention uses the technique of semisolid enzymatic hydrolysis, can produce the grain dust containing polypeptide, the water content phase of material To lower, after subsequent enzymatic hydrolysis in drying process, the moisture of required evaporation is greatly lowered, and can reduce production cost.In addition, The process of semisolid enzymolysis process is simple, and equipment requirement is not high, and production technology is easily controllable.It avoids using spray drying or rolling The more complicated drying programs such as cylinder drying, significantly reduce power consumption of polymer processing and processing cost, are conducive to heavy industrialization Production.In addition, the cutting of Harmonic Protein Molecular Chains is more mild since semisolid is digested compared to liquid phase enzymolysis process, it can be compared with The molecular weight distribution for controlling polypeptide well reduces the generation of bitter peptides, so as to improve the smell containing polypeptide grain dust and Flavour makes it have better market acceptance.
Detailed description of the invention
Fig. 1 and Fig. 2 is respectively the production method flow diagram of semisolid enzymatic hydrolysis grain dust of the present invention.
Specific embodiment
The following examples are used to illustrate the present invention, but are not intended to limit the scope of the present invention..It is not specified in embodiment specific Technology or conditions person, described technology or conditions according to the literature in the art, or carried out according to product description.It is used Production firm person is not specified in reagent or instrument, is the conventional products that can be commercially available by regular distributor.
Alcalase used below is the Alcalase 2.4L purchased from Novozymes Company;Neutrase used is purchased from promise The Neutrase 0.8L of Wei Xin company.Oatmeal partial size used below is 200 mesh, and red bean powder partial size used is 200 mesh.
The production method flow diagram of semisolid enzymatic hydrolysis grain dust of the present invention is shown in Fig. 1 and Fig. 2 (containing the step for mending powder Suddenly).
Embodiment 1
The production method of the oatmeal containing polypeptide, specific as follows:
1) raw material mixes: after enzyme preparation, sodium bicarbonate are mixed with water, being added in oatmeal, is uniformly mixed, forms dough State makes the water content 46% of material after mixing, and enzyme preparation uses Novozymes Company Alcalase, and dosage accounts for oatmeal ratio 0.5wt%;And Neutrase, dosage account for oatmeal ratio 0.25wt%, sodium bicarbonate accounts for oatmeal ratio 0.5wt%;
2) temperature control digests: under the conditions of hydrolysis temperature (65 DEG C), constant temperature stirs certain time (2h);
3) mend powder: enzymatic hydrolysis proceeds to 2h, supplements the oatmeal dry powder of 20% weight, then proceedes to reaction 0.5h;
4) it forms: after enzymatic hydrolysis, passing the dough through screw extruding mode and be molded into little particle state;
5) enzyme deactivation is dried: little particle after molding being handled certain time (10min) under enzyme-removal temperature (110 DEG C), so Processing a period of time (30min) is dried under drying temperature (80 DEG C) again afterwards;
6) it crushes: the little particle after drying is ground into powdery to get oatmeal containing polypeptide.
Comparative example 1
It is specific as follows up to the liquid enzymolysis process of the oatmeal containing polypeptide:
1) raw material mixes: after enzyme preparation, sodium bicarbonate are mixed with water, adding according to the ratio of water and oatmeal mass ratio 4:1 Enter in oatmeal, stir evenly, forming face pulpous state state, making the water content of material after mixing is about 80%, and enzyme preparation uses Novi Letter company Alcalase, dosage account for oatmeal ratio 0.5wt%;And Neutrase, dosage account for oatmeal ratio 0.25wt%, Sodium bicarbonate accounts for oatmeal ratio 0.5wt%;
2) temperature control digests: under the conditions of hydrolysis temperature (65 DEG C), constant temperature stirs certain time (2.5h);
3) starch adhesive after enzymatic hydrolysis enzyme deactivation: is handled into certain time (10min) under enzyme-removal temperature (110 DEG C);
4) it is spray-dried: powder is made by spray drying tower to get oatmeal containing polypeptide in the starch adhesive after enzyme deactivation.
Oatmeal containing polypeptide prepared by embodiment 1 and comparative example 1 test results in the following table:
1 liquid enzymolysis process of comparative example The embodiment of the present invention 1
Content of peptides 8.8% 8.6%
Proteolysis ratio 58.7% 57.3%
Production cost 8-10 member/kg 2.5 yuan/kg
The above result shows that the content of peptides of the obtained oatmeal containing polypeptide of the embodiment of the present invention 1 and comparative example 1 use Liquid enzymolysis process is almost the same, and still, the semisolid enzymolysis process that the embodiment of the present invention 1 uses can be big by production cost Width is reduced to 2.5 yuan/kg, facilitates production and popularization and application containing polypeptide oatmeal.
Embodiment 2
The production method of the red bean powder containing polypeptide, specific as follows:
1) raw material mixes: after enzyme preparation, sodium bicarbonate are mixed with water, being added in red bean powder, is uniformly mixed, forms dough State, the moisture content of material is 47% after mixing, and enzyme preparation uses Novozymes Company Alcalase, and dosage accounts for red bean powder ratio 0.5wt% and neutrase, dosage account for red bean powder ratio 0.25wt%, and sodium bicarbonate accounts for oatmeal ratio 0.5wt%;
2) temperature control digests: under the conditions of hydrolysis temperature (65 DEG C), constant temperature stirs certain time (1.5h);
3) mend powder: enzymatic hydrolysis proceeds to 1.5h, supplements 10% red bean powder dry powder, then proceedes to reaction 0.5h;
4) it forms: after enzymatic hydrolysis, passing the dough through screw extruding mode and be molded into little particle state;
5) enzyme deactivation is dried: little particle after molding being handled certain time (10min) under enzyme-removal temperature (110 DEG C), so Processing a period of time (30min) is dried under drying temperature (80 DEG C) again afterwards;
6) it crushes: the little particle after drying is ground into powdery to get red bean powder containing polypeptide.
Comparative example 2
The liquid enzymolysis process of the red bean powder containing polypeptide, specific as follows:
1) raw material mixes: after enzyme preparation, sodium bicarbonate are mixed with water, adding according to the ratio of water and red bean powder mass ratio 9:1 Enter in red bean powder, stir evenly, forming face pulpous state state, making the water content of material after mixing is about 90%, and enzyme preparation uses Novi Letter company Alcalase, dosage account for red bean powder ratio 0.5wt%;And Neutrase, dosage account for red bean powder ratio 0.25wt%, Sodium bicarbonate accounts for red bean powder ratio 0.5wt%;
2) temperature control digests: under the conditions of hydrolysis temperature (65 DEG C), constant temperature stirs certain time (2h);
3) starch adhesive after enzymatic hydrolysis enzyme deactivation: is handled into certain time (10min) under enzyme-removal temperature (110 DEG C);
4) it is spray-dried: powder is made by spray drying tower to get red bean powder containing polypeptide in the starch adhesive after enzyme deactivation.
Oatmeal containing polypeptide prepared by embodiment 2 and comparative example 2 test results in the following table:
2 liquid enzymolysis process of comparative example The embodiment of the present invention 2
Content of peptides 13.5% 12.8%
Proteolysis ratio 67.5% 64%
Production cost 8-10 member/kg 2.4 yuan/kg
The content of peptides and comparative example 2 for showing the obtained red bean powder containing polypeptide of the embodiment of the present invention 2 are digested using liquid Technique is almost the same, still, the embodiment of the present invention 2 use semisolid enzymolysis process, production cost can be greatly reduced to 2.4 yuan/kg, facilitate production and popularization and application containing polypeptide red bean powder.
Experimental example
Oatmeal containing polypeptide obtained by above-described embodiment 1-2 and comparative example 1-2, red bean powder containing polypeptide are carried out artificial Sensory evaluation experiment.Specific method: according to grain dust containing polypeptide: warm water=1:8 weight configures the solution of grain dust containing polypeptide, The sensory evaluation group being made of 6 people carries out sensory evaluation to sample at random according to following table standard:
Sensory evaluation criteria
Sensory evaluation scoring is averaged, and evaluation result is as shown in the table:
By the above evaluation result it is found that the grain dust containing polypeptide of semisolid enzymolysis process preparation digests on sense organ compared to liquid phase Product, in terms of the smell of sensory evaluation, flavour and overall acceptance have big advantage.Although above It, can be to it through the present invention is described in detail with a general description of the specific embodiments, but on the basis of the present invention It makes some modifications or improvements, this will be apparent to those skilled in the art.Therefore, without departing from spirit of that invention On the basis of these modifications or improvements, fall within the scope of the claimed invention.

Claims (10)

1. a kind of production method of semisolid enzymatic hydrolysis grain dust, comprising: be uniformly mixed enzyme preparation, water and grain dust, make At dough state, make the water content 30%-55% of material after mixing;It is digested under enzymatic hydrolysis condition appropriate;Alternatively, also Including little particle state is made in the material after enzymatic hydrolysis, enzyme deactivation be dry, pulverize.
2. production method according to claim 1, which is characterized in that the enzyme preparation includes protease, preferably includes wood At least one of melon protease, bacterialprotease, carboxypeptidase, trypsase, beta lipoprotein enzyme;And/or
Preferably, the dosage of the enzyme preparation is the 0.1-2%, more preferably 0.5-1% of grain dust weight.
3. production method according to claim 1, which is characterized in that the enzyme preparation is Novozymes Company Alcalase; And/or Novozymes Company Neutrase;
Preferably, the dosage of the Alcalase be grain dust weight 0.2-2%, more preferably 0.5%;And/or preferably Ground, the dosage of Neutrase are the 0.1-2% of grain dust weight, more preferably 0.25%.
4. production method according to claim 1-3, which is characterized in that make the water content of material after mixing 40%-55%.
5. production method according to claim 1-4, which is characterized in that the partial size of the grain dust is 60-300 Mesh, preferably 100-200 mesh;And/or
The cereal materials include one or more of oatmeal, red bean powder, buckwheat, soy meal;And/or
Material after enzymatic hydrolysis is made to the little particle state of partial size 0.5-5mm.
6. production method according to claim 1-5, which is characterized in that during dough is made or digested Appropriate sodium bicarbonate is additionally added in journey;
Preferably, the dosage of sodium bicarbonate is the 0.1-2%, more preferably 0.5-1.5% of grain dust.
7. production method according to claim 1-6, which is characterized in that the hydrolysis temperature is 60-65 DEG C, enzyme The solution time is 0.5-4h.
8. production method according to claim 1-7, which is characterized in that further include terminating previous timing in enzymatic hydrolysis Between (preferably 20-40min, more preferable 30min or so) be supplemented the dried grains powder of a certain amount of (preferably 5%-30% weight), mixing Uniformly, continue to digest.
9. production method according to claim 1-8, which is characterized in that, comprising: by enzyme preparation, sodium bicarbonate, water It is uniformly mixed, is added in the grain dust of 60-300 mesh, be uniformly mixed, dough state is made, make the water content of material after mixing 30%-55%;It is digested under enzymatic hydrolysis condition appropriate;20-40min is supplemented the dry of 5%-30% weight before enzymatic hydrolysis terminates Grain dust is uniformly mixed;Continue to digest;Material after enzymatic hydrolysis is made to the little particle state of partial size 0.5-5mm, enzyme deactivation, It dry, pulverize.
10. the hydrolyzed cereal powder of any one of claim 1-9 the method preparation;
Preferably, the content of peptides of the hydrolyzed cereal powder is up to 5-15%, and proteolysis ratio is up to 50-65%.
CN201810663113.8A 2018-06-25 2018-06-25 A kind of semisolid enzymatic hydrolysis grain dust and its production method Pending CN109043307A (en)

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CN113397091A (en) * 2021-06-07 2021-09-17 武汉轻工大学 Bran modification method and gluten-containing flour product

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