A kind of semisolid enzymatic hydrolysis grain dust and its production method
Technical field
The invention belongs to technical field of agricultural product process, especially grain dust product processing technique field.
Background technique
Corn gluten protein hydrolysis generate polypeptide, have it is anti-oxidant, blood pressure lowering and promote fat metabolism, reduce cholesterol,
Antifatigue and adjust the functions such as immune, the functional nutrient that can greatly improve cereal is horizontal, and more high added value has been provided in creation
Product.Currently used enzymolysis process is carried out in liquid cereal slurry material, and process is various, enzymolysis process time-consuming compared with
Long, subsequent enzyme deactivation needs higher energy input;Cereal needs complex using spray drying or roller drying etc. after enzymatic hydrolysis
Drying program so that processing cost is higher, energy consumption is high, is unfavorable for large-scale industrial production.
Current existing cereal enzymolysis scheme is carried out using liquid enzyme solution.Traditional enzymolysis process is liquid
Enzymatic isolation method, using large-size reactor, batch production, i.e. one pot one pot of production.Water content is very high when enzymatic hydrolysis, 70-90% with
On.There is no Ultramicro Dispersion complete sets of Techniques, filtering technique therefore, after enzymatic hydrolysis is taken to filter off cellulose components.Then, thickening.
Finally using spray drying or roller drying, enzymatic hydrolysis grain dust is made.There is process flow complexity, workshops for cylinder drying
The problems such as personnel are more, and equipment is more, take up a large area, water, electricity, higher vapour consumption.Liquid process process energy consumption are as follows: agitating and heating section
100 degree/t, it is spray-dried 2500 degree/t of section, adds up to 2600 degree/t, energy consumption is huge, and increased costs are very high, reaches per kilogram
8-10 member or so.Simultaneously according to the technique, production main process is wet production process, and roller drying production line has huge work
In air, Environmental microorganism control requires height to the exposure of industry face, and workshop needs to fit up and run in strict accordance with GMP, and investment is huge;
And production line flow process is long, and equipment is huge, compares suitable for producing single variety, it is relatively difficult that different grain varieties change production.Roller
Dry processing technology also causes grain dry temperature low, and the fragrance of product is very weak.The prior art uses liquid enzymolysis process, raw
Production cost is very high, and product is difficult to be applied as ordinary food ingredient.In addition, the cereal produced using liquid enzymolysis process
Powder has serious bitter taste, it is difficult to carry out food applications, greatly limit marketing due to the generation of bitter peptides.
Summary of the invention
In order to solve the above technical problems, the present invention provides a kind of semisolid enzymatic hydrolysis grain dust and its production method, adopt
So that raw material is in semisolid, the i.e. state of dough in enzymolysis process with the technique that semisolid digests, by enzyme preparation and
The control of technique can reach higher polypeptide yield, and production cost is greatly lowered, and improve product special flavour.
Technical solution of the present invention is as follows:
A kind of production method of semisolid enzymatic hydrolysis grain dust, comprising: enzyme preparation, water and grain dust are uniformly mixed,
Dough state is made, makes the water content 30%-55% of material after mixing;It is digested under enzymatic hydrolysis condition appropriate;Alternatively,
It further include that little particle state is made in the material after enzymatic hydrolysis, enzyme deactivation dry, pulverize.
Further, the enzyme preparation includes protease, specifically includes papain, bacterialprotease, carboxypeptidase, pancreas
At least one of protease, beta lipoprotein enzyme etc..
Further, the dosage of the enzyme preparation (referring mainly to protease) is the 0.1-2% of grain dust weight, preferably
0.5-1%.
Specifically, commercialized enzyme preparation, such as Novozymes Company Alcalase (alkali protease), Novi's letter can be selected
One or more of company Neutrase (neutral proteinase) etc..Preferably, the dosage of Alcalase is grain dust weight
0.2-2%, more preferably 0.5%;And/or, it is preferable that the dosage of Neutrase is the 0.1-2% of grain dust weight, more preferably
It is 0.25%.
Further, appropriate sodium bicarbonate can also be added during dough is made or in enzymolysis process, preferably to promote
Into the progress of enzymolysis process.Its dosage can be determined according to used enzyme preparation and/or cereal materials used, generally, little Su
The dosage beaten can be the 0.1-2%, preferably 0.5-1.5% of grain dust weight.
Specifically, it after can first mixing enzyme preparation, sodium bicarbonate with water, adds in grain dust.
Preferably to improve hydrolysis result, grain dust can be crushed to 60-300 mesh, preferably 100-200 mesh.
Further, the cereal materials include one or more of oatmeal, red bean powder, buckwheat, soy meal etc..
Further, the water content 40%-55% of material after mixing can be made.
Enzymatic hydrolysis condition can be determined according to used enzyme preparation and cereal materials used, under normal circumstances can be in temperature 60-
Under the conditions of 65 DEG C, 0.5-4h is digested.
Further, the material after enzymatic hydrolysis can be made to the little particle state of partial size 0.5-5mm, in order to subsequent step
Rapid processing.
Enzyme deactivation condition can also be determined according to used enzyme preparation and/or cereal materials used, under normal circumstances can be in temperature
Under the conditions of 100-120 DEG C of degree, 5-20min is digested.
Drying condition can also be determined according to used enzyme preparation and/or cereal materials used, under normal circumstances can be in temperature
Dry 10-60min under the conditions of 60-100 DEG C of degree.
It can also be as needed by the crushing material after drying to required partial size.
Further, can before enzymatic hydrolysis terminates certain time (such as 20-40min, preferably 30min or so) be supplemented centainly
The dried grains powder of (such as 5%-30% weight) is measured, is uniformly mixed, continues to digest;Material texture hardness can be made to improve, it is easier to
Little particle state is made to be more easily fabricated into molding.
Specifically, the production method of above-mentioned semisolid enzymatic hydrolysis grain dust, comprising: mix enzyme preparation, sodium bicarbonate, water
Uniformly, it is added in the grain dust of 60-300 mesh, is uniformly mixed, dough state is made, make the water content 30%- of material after mixing
55%;It is digested under enzymatic hydrolysis condition appropriate;20-40min is supplemented the dried grains of 5%-30% weight before enzymatic hydrolysis terminates
Powder is uniformly mixed;Continue to digest;Material after enzymatic hydrolysis is made to the little particle state of partial size 0.5-5mm, enzyme deactivation is dry,
It crushes.
The invention also includes the hydrolyzed cereal powder of the method preparation.Its content of peptides is up to 5-15%, proteolysis ratio
Example is up to 50-65%.
The raw materials used in the present invention is commercially available to buy, or prepares by conventional method in that art.
On the basis of common knowledge of the art, above-mentioned each optimum condition can be combined with each other each preferably to get the present invention
Example.
The present invention uses the technique of semisolid enzymatic hydrolysis, can produce the grain dust containing polypeptide, the water content phase of material
To lower, after subsequent enzymatic hydrolysis in drying process, the moisture of required evaporation is greatly lowered, and can reduce production cost.In addition,
The process of semisolid enzymolysis process is simple, and equipment requirement is not high, and production technology is easily controllable.It avoids using spray drying or rolling
The more complicated drying programs such as cylinder drying, significantly reduce power consumption of polymer processing and processing cost, are conducive to heavy industrialization
Production.In addition, the cutting of Harmonic Protein Molecular Chains is more mild since semisolid is digested compared to liquid phase enzymolysis process, it can be compared with
The molecular weight distribution for controlling polypeptide well reduces the generation of bitter peptides, so as to improve the smell containing polypeptide grain dust and
Flavour makes it have better market acceptance.
Detailed description of the invention
Fig. 1 and Fig. 2 is respectively the production method flow diagram of semisolid enzymatic hydrolysis grain dust of the present invention.
Specific embodiment
The following examples are used to illustrate the present invention, but are not intended to limit the scope of the present invention..It is not specified in embodiment specific
Technology or conditions person, described technology or conditions according to the literature in the art, or carried out according to product description.It is used
Production firm person is not specified in reagent or instrument, is the conventional products that can be commercially available by regular distributor.
Alcalase used below is the Alcalase 2.4L purchased from Novozymes Company;Neutrase used is purchased from promise
The Neutrase 0.8L of Wei Xin company.Oatmeal partial size used below is 200 mesh, and red bean powder partial size used is 200 mesh.
The production method flow diagram of semisolid enzymatic hydrolysis grain dust of the present invention is shown in Fig. 1 and Fig. 2 (containing the step for mending powder
Suddenly).
Embodiment 1
The production method of the oatmeal containing polypeptide, specific as follows:
1) raw material mixes: after enzyme preparation, sodium bicarbonate are mixed with water, being added in oatmeal, is uniformly mixed, forms dough
State makes the water content 46% of material after mixing, and enzyme preparation uses Novozymes Company Alcalase, and dosage accounts for oatmeal ratio
0.5wt%;And Neutrase, dosage account for oatmeal ratio 0.25wt%, sodium bicarbonate accounts for oatmeal ratio 0.5wt%;
2) temperature control digests: under the conditions of hydrolysis temperature (65 DEG C), constant temperature stirs certain time (2h);
3) mend powder: enzymatic hydrolysis proceeds to 2h, supplements the oatmeal dry powder of 20% weight, then proceedes to reaction 0.5h;
4) it forms: after enzymatic hydrolysis, passing the dough through screw extruding mode and be molded into little particle state;
5) enzyme deactivation is dried: little particle after molding being handled certain time (10min) under enzyme-removal temperature (110 DEG C), so
Processing a period of time (30min) is dried under drying temperature (80 DEG C) again afterwards;
6) it crushes: the little particle after drying is ground into powdery to get oatmeal containing polypeptide.
Comparative example 1
It is specific as follows up to the liquid enzymolysis process of the oatmeal containing polypeptide:
1) raw material mixes: after enzyme preparation, sodium bicarbonate are mixed with water, adding according to the ratio of water and oatmeal mass ratio 4:1
Enter in oatmeal, stir evenly, forming face pulpous state state, making the water content of material after mixing is about 80%, and enzyme preparation uses Novi
Letter company Alcalase, dosage account for oatmeal ratio 0.5wt%;And Neutrase, dosage account for oatmeal ratio 0.25wt%,
Sodium bicarbonate accounts for oatmeal ratio 0.5wt%;
2) temperature control digests: under the conditions of hydrolysis temperature (65 DEG C), constant temperature stirs certain time (2.5h);
3) starch adhesive after enzymatic hydrolysis enzyme deactivation: is handled into certain time (10min) under enzyme-removal temperature (110 DEG C);
4) it is spray-dried: powder is made by spray drying tower to get oatmeal containing polypeptide in the starch adhesive after enzyme deactivation.
Oatmeal containing polypeptide prepared by embodiment 1 and comparative example 1 test results in the following table:
|
1 liquid enzymolysis process of comparative example |
The embodiment of the present invention 1 |
Content of peptides |
8.8% |
8.6% |
Proteolysis ratio |
58.7% |
57.3% |
Production cost |
8-10 member/kg |
2.5 yuan/kg |
The above result shows that the content of peptides of the obtained oatmeal containing polypeptide of the embodiment of the present invention 1 and comparative example 1 use
Liquid enzymolysis process is almost the same, and still, the semisolid enzymolysis process that the embodiment of the present invention 1 uses can be big by production cost
Width is reduced to 2.5 yuan/kg, facilitates production and popularization and application containing polypeptide oatmeal.
Embodiment 2
The production method of the red bean powder containing polypeptide, specific as follows:
1) raw material mixes: after enzyme preparation, sodium bicarbonate are mixed with water, being added in red bean powder, is uniformly mixed, forms dough
State, the moisture content of material is 47% after mixing, and enzyme preparation uses Novozymes Company Alcalase, and dosage accounts for red bean powder ratio
0.5wt% and neutrase, dosage account for red bean powder ratio 0.25wt%, and sodium bicarbonate accounts for oatmeal ratio 0.5wt%;
2) temperature control digests: under the conditions of hydrolysis temperature (65 DEG C), constant temperature stirs certain time (1.5h);
3) mend powder: enzymatic hydrolysis proceeds to 1.5h, supplements 10% red bean powder dry powder, then proceedes to reaction 0.5h;
4) it forms: after enzymatic hydrolysis, passing the dough through screw extruding mode and be molded into little particle state;
5) enzyme deactivation is dried: little particle after molding being handled certain time (10min) under enzyme-removal temperature (110 DEG C), so
Processing a period of time (30min) is dried under drying temperature (80 DEG C) again afterwards;
6) it crushes: the little particle after drying is ground into powdery to get red bean powder containing polypeptide.
Comparative example 2
The liquid enzymolysis process of the red bean powder containing polypeptide, specific as follows:
1) raw material mixes: after enzyme preparation, sodium bicarbonate are mixed with water, adding according to the ratio of water and red bean powder mass ratio 9:1
Enter in red bean powder, stir evenly, forming face pulpous state state, making the water content of material after mixing is about 90%, and enzyme preparation uses Novi
Letter company Alcalase, dosage account for red bean powder ratio 0.5wt%;And Neutrase, dosage account for red bean powder ratio 0.25wt%,
Sodium bicarbonate accounts for red bean powder ratio 0.5wt%;
2) temperature control digests: under the conditions of hydrolysis temperature (65 DEG C), constant temperature stirs certain time (2h);
3) starch adhesive after enzymatic hydrolysis enzyme deactivation: is handled into certain time (10min) under enzyme-removal temperature (110 DEG C);
4) it is spray-dried: powder is made by spray drying tower to get red bean powder containing polypeptide in the starch adhesive after enzyme deactivation.
Oatmeal containing polypeptide prepared by embodiment 2 and comparative example 2 test results in the following table:
|
2 liquid enzymolysis process of comparative example |
The embodiment of the present invention 2 |
Content of peptides |
13.5% |
12.8% |
Proteolysis ratio |
67.5% |
64% |
Production cost |
8-10 member/kg |
2.4 yuan/kg |
The content of peptides and comparative example 2 for showing the obtained red bean powder containing polypeptide of the embodiment of the present invention 2 are digested using liquid
Technique is almost the same, still, the embodiment of the present invention 2 use semisolid enzymolysis process, production cost can be greatly reduced to
2.4 yuan/kg, facilitate production and popularization and application containing polypeptide red bean powder.
Experimental example
Oatmeal containing polypeptide obtained by above-described embodiment 1-2 and comparative example 1-2, red bean powder containing polypeptide are carried out artificial
Sensory evaluation experiment.Specific method: according to grain dust containing polypeptide: warm water=1:8 weight configures the solution of grain dust containing polypeptide,
The sensory evaluation group being made of 6 people carries out sensory evaluation to sample at random according to following table standard:
Sensory evaluation criteria
Sensory evaluation scoring is averaged, and evaluation result is as shown in the table:
By the above evaluation result it is found that the grain dust containing polypeptide of semisolid enzymolysis process preparation digests on sense organ compared to liquid phase
Product, in terms of the smell of sensory evaluation, flavour and overall acceptance have big advantage.Although above
It, can be to it through the present invention is described in detail with a general description of the specific embodiments, but on the basis of the present invention
It makes some modifications or improvements, this will be apparent to those skilled in the art.Therefore, without departing from spirit of that invention
On the basis of these modifications or improvements, fall within the scope of the claimed invention.