CN109042745A - Green prevention and control bacterium solution for graw mold of tomato - Google Patents
Green prevention and control bacterium solution for graw mold of tomato Download PDFInfo
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- CN109042745A CN109042745A CN201811069252.4A CN201811069252A CN109042745A CN 109042745 A CN109042745 A CN 109042745A CN 201811069252 A CN201811069252 A CN 201811069252A CN 109042745 A CN109042745 A CN 109042745A
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- Prior art keywords
- tomato
- thallus laminariae
- liquid
- bacterium solution
- graw mold
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N65/00—Biocides, pest repellants or attractants, or plant growth regulators containing material from algae, lichens, bryophyta, multi-cellular fungi or plants, or extracts thereof
- A01N65/03—Algae
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N63/00—Biocides, pest repellants or attractants, or plant growth regulators containing microorganisms, viruses, microbial fungi, animals or substances produced by, or obtained from, microorganisms, viruses, microbial fungi or animals, e.g. enzymes or fermentates
- A01N63/10—Animals; Substances produced thereby or obtained therefrom
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N63/00—Biocides, pest repellants or attractants, or plant growth regulators containing microorganisms, viruses, microbial fungi, animals or substances produced by, or obtained from, microorganisms, viruses, microbial fungi or animals, e.g. enzymes or fermentates
- A01N63/30—Microbial fungi; Substances produced thereby or obtained therefrom
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- Engineering & Computer Science (AREA)
- Health & Medical Sciences (AREA)
- General Health & Medical Sciences (AREA)
- Zoology (AREA)
- Biotechnology (AREA)
- Microbiology (AREA)
- Environmental Sciences (AREA)
- Agronomy & Crop Science (AREA)
- Plant Pathology (AREA)
- Dentistry (AREA)
- Wood Science & Technology (AREA)
- Mycology (AREA)
- Pest Control & Pesticides (AREA)
- Virology (AREA)
- Natural Medicines & Medicinal Plants (AREA)
- Agricultural Chemicals And Associated Chemicals (AREA)
Abstract
Crop pest management technical field of the present invention, in particular to a kind of green prevention and control bacterium solution for graw mold of tomato.The green prevention and control bacterium solution for being used for graw mold of tomato is crushed liquid with thallus laminariae by koning trichoderma fermentation liquid and mixes by the volume ratio of 1:1, and 8~10g of trichoderma dry mycelial weight is contained in every liter of koning trichoderma fermentation liquid, and every liter of thallus laminariae, which is crushed in liquid, contains 20~30g of thallus laminariae dry weight.The present invention is only realized with a kind of strain of Trichoderma and is effectively prevented to graw mold of tomato, and safe and non-toxic.It is convenient that thallus laminariae is crushed liquid preparation, and cost is relatively low, keeps the processing of finished product more convenient.
Description
Technical field
Crop pest management technical field of the present invention, in particular to a kind of green prevention and control bacterium for graw mold of tomato
Liquid.
Background technique
Gray mold is that tomato is common, endangers heavier disease, and germ mainly encroaches on fruit, and pericarp is made to become canescence water
Stain shape rots until softening, and occurs the mould layer of a large amount of taupe on fruit face, calyx and carpopodium later, finally makes fruit dehydration stiff
Change.In addition, iron deficiency phenomenon often occurs for tomato plant, cause top leaf chlorosis, old leaf develops to the lower part from top, and has slight
Tissue necrosis.When iron deficiency occurs, blade face can be sprayed with 0.5%~1% ferrous sulfate solution.
Thallus laminariae is a kind of seaweed, outer likeness in form kelp, dark brown, grows nonparasitically upon another plant on rock reef, the coastal areas such as Fujian, Zhejiang are equal more
It is distributed.Thallus laminariae not only has higher medical value and anti-inflammation detumescence effect or a kind of food materials of deliciousness, but disposable food
Thallus laminariae is excessive, is easy to cause abdominal distension.
Trichoderma is a kind of important biocontrol fungi, to the prevention and treatment important role of soil-borne disease, Biocontrol Mechanism
Including bacteriolysis, Competition, generation antibiotic etc..Trichoderma is only used for the prevention before the onset of crop, once morbidity, disease
After opportunistic pathogen enters in plant, only it is unable to cope with by Trichoderma.So in common biocontrol agent, Trichoderma with other bacterium
Strain is used cooperatively.108203703 A of Chinese patent literature CN discloses a kind of biocontrol agent and its answering in prevention and treatment gray mold
With comprising trichosporon cutaneum, koning trichoderma and Pseudomonas stutzeri reach 85% to the inhibiting rate of graw mold of tomato pathogen
Left and right.
Summary of the invention
It is an object of the invention to overcome the deficiencies of the prior art and provide a kind of green prevention and control bacterium for graw mold of tomato
Liquid has preferable control efficiency to graw mold of tomato, and to people and animals' nonhazardous.
To achieve the goals above, technical scheme is as follows: for the green prevention and control bacterium solution of graw mold of tomato,
Liquid is crushed by koning trichoderma fermentation liquid and thallus laminariae to mix.
As an improvement, in the koning trichoderma fermentation liquid, in every liter of koning trichoderma fermentation liquid containing trichoderma dry mycelial weight 8~
10g。
As an improvement, the thallus laminariae is crushed in liquid, every liter of thallus laminariae, which is crushed in liquid, contains 20~30g of thallus laminariae dry weight.
It is mixed as an improvement, the koning trichoderma fermentation liquid is crushed liquid with thallus laminariae by the volume ratio of 1:1.
As an improvement, the thallus laminariae is crushed in liquid, the fineness of thallus laminariae is 60~80 mesh.
As an improvement, the thallus laminariae is crushed in liquid added with thermophilic iron element.
The beneficial effects of the present invention are: koning trichoderma fermentation liquid is by being crushed being used cooperatively for liquid with thallus laminariae, it is no longer necessary to
With other strain compatibilities such as bacillus, is only realized with a kind of strain of Trichoderma and graw mold of tomato is effectively prevented, and
It is safe and non-toxic.It is convenient that thallus laminariae is crushed liquid preparation, and cost is relatively low, keeps the processing of finished product more convenient.
Specific embodiment
Embodiment 1
For the green prevention and control bacterium solution of graw mold of tomato, the volume ratio that liquid presses 1:1 is crushed by koning trichoderma fermentation liquid and thallus laminariae
It mixes, 8~10g of trichoderma dry mycelial weight is contained in every liter of koning trichoderma fermentation liquid, every liter of thallus laminariae, which is crushed in liquid, contains thallus laminariae
20~30g of dry weight.
The preparation step of koning trichoderma fermentation liquid is as follows: koning trichoderma streak inoculation being cultivated in PDA culture medium, is obtained
Single colonie, picking single colonie are inoculated into PDA liquid medium and cultivate, 30 DEG C, 200rpm shaking table culture 36h, obtain Kang Shi wood
Mould seed liquor.Koning trichoderma seed liquor is linked into fermentation medium according to 10% inoculum concentration, and 30 DEG C of cultures obtain health in 36 hours
Family name's trichoderma fermentation liquid, the formula of fermentation medium are as follows: corn stalk powder 16g/L, dregs of beans 10g/L, ammonium chloride 5g/L, phosphoric acid hydrogen two
Potassium 2g/L, potassium dihydrogen phosphate 1g/L, 0 .5g/L of sodium chloride, 0 .1g/L of magnesium sulfate, 0 .01g/L of ferrous sulfate.
The preparation step that thallus laminariae is crushed liquid is as follows: after the weighing of dry thallus laminariae, adding appropriate amount of water, carries out ultrasonication, make thallus laminariae
Fineness be 60~80 mesh, later plus water is crushed every liter of thallus laminariae in liquid containing 20~30g of thallus laminariae dry weight.
Koning trichoderma fermentation liquid and thallus laminariae are crushed liquid after mixing to get arriving cost.
Effect experiment: in by the end of April, 2018, and gray mold, grey mold occurs in the tomato in the farm of Huzhou City of Zhejiang Province Nanxun District
Disease betides the holder of tomato.Applicant does comparative experiments: being sprayed at one with green prevention and control bacterium solution, green prevention and control bacterium solution is diluted
It is sprayed at morbidity, was sprayed every 2 hours once for spray 3 times after 10 times；It is sprayed at one with koning trichoderma fermentation liquid, dilution times
Number, to spray frequency identical as green prevention and control bacterium solution；It is crushed liquid with thallus laminariae at one to spray, extension rate, to spray frequency and green anti-
It is identical to control bacterium solution.Result is observed after 12 hours: spraying the tomato of green prevention and control bacterium solution, and the state of an illness is clearly better；Only spray Kang Shi wood
The tomato of mould fermentation liquid, the state of an illness is without significant change；The tomato that thallus laminariae is crushed liquid is only sprayed, gray mold has been developed to connect with holder
Pulp at, pericarp be in canescence.Illustrate that koning trichoderma and thallus laminariae have synergistic effect to processing graw mold of tomato.
Embodiment 2
The present embodiment difference from example 1 is that: the thallus laminariae is crushed in liquid added with thermophilic iron element.Thermophilic iron element is a kind of
The small-molecular-weight synthesized under the conditions of low iron by microorganism, can specifically chelate Fe3+Small molecule compound.Iron member in thallus laminariae
The content of element is higher, and thermophilic iron element is partially fallen in soil during spraying with green prevention and control bacterium solution, and thermophilic iron element can promote kind
Absorption of the eggplant to ferro element adds the resistance of tomato plant itself to play the role of promoting tomato plant strain growth
By force.
Claims (6)
1. being used for the green prevention and control bacterium solution of graw mold of tomato, it is characterised in that: it is crushed liquid by koning trichoderma fermentation liquid and thallus laminariae
It mixes.
2. being used for the green prevention and control bacterium solution of graw mold of tomato as described in claim 1, it is characterised in that: the koning trichoderma hair
In zymotic fluid, 8~10g of trichoderma dry mycelial weight is contained in every liter of koning trichoderma fermentation liquid.
3. being used for the green prevention and control bacterium solution of graw mold of tomato as claimed in claim 1 or 2, it is characterised in that: the thallus laminariae is broken
In broken liquid, every liter of thallus laminariae, which is crushed in liquid, contains 20~30g of thallus laminariae dry weight.
4. being used for the green prevention and control bacterium solution of graw mold of tomato as claimed in claim 3, it is characterised in that: the koning trichoderma hair
Zymotic fluid is crushed liquid with thallus laminariae and mixes by the volume ratio of 1:1.
5. being used for the green prevention and control bacterium solution of graw mold of tomato as described in claim 1, it is characterised in that: the thallus laminariae is crushed liquid
In, the fineness of thallus laminariae is 60~80 mesh.
6. being used for the green prevention and control bacterium solution of graw mold of tomato as described in claim 1, it is characterised in that: the thallus laminariae is crushed liquid
In added with thermophilic iron element.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201811069252.4A CN109042745A (en) | 2018-09-13 | 2018-09-13 | Green prevention and control bacterium solution for graw mold of tomato |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201811069252.4A CN109042745A (en) | 2018-09-13 | 2018-09-13 | Green prevention and control bacterium solution for graw mold of tomato |
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| Publication Number | Publication Date |
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| CN109042745A true CN109042745A (en) | 2018-12-21 |
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| Application Number | Title | Priority Date | Filing Date |
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| CN201811069252.4A Pending CN109042745A (en) | 2018-09-13 | 2018-09-13 | Green prevention and control bacterium solution for graw mold of tomato |
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Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101027990A (en) * | 2007-03-13 | 2007-09-05 | 西北农林科技大学无公害农药研究服务中心 | Bactericidal use of asarone for preventing and treating plant diseases caused by plant pathogenic bacteria |
| CN101099496A (en) * | 2007-07-27 | 2008-01-09 | 浙江林学院 | Gale branch and leaves extraction disinfectant and its producing method |
| CN101485316A (en) * | 2009-03-04 | 2009-07-22 | 中国农业大学 | Compositional bactericidal agent for preventing and treating gray mold |
| CN108203703A (en) * | 2018-03-26 | 2018-06-26 | 叶志坚 | A kind of biocontrol agent and its application in gray mold is prevented |
| CN108293480A (en) * | 2018-03-26 | 2018-07-20 | 叶志坚 | The method for preventing graw mold of tomato using biocontrol agent |
-
2018
- 2018-09-13 CN CN201811069252.4A patent/CN109042745A/en active Pending
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101027990A (en) * | 2007-03-13 | 2007-09-05 | 西北农林科技大学无公害农药研究服务中心 | Bactericidal use of asarone for preventing and treating plant diseases caused by plant pathogenic bacteria |
| CN101099496A (en) * | 2007-07-27 | 2008-01-09 | 浙江林学院 | Gale branch and leaves extraction disinfectant and its producing method |
| CN101485316A (en) * | 2009-03-04 | 2009-07-22 | 中国农业大学 | Compositional bactericidal agent for preventing and treating gray mold |
| CN108203703A (en) * | 2018-03-26 | 2018-06-26 | 叶志坚 | A kind of biocontrol agent and its application in gray mold is prevented |
| CN108293480A (en) * | 2018-03-26 | 2018-07-20 | 叶志坚 | The method for preventing graw mold of tomato using biocontrol agent |
Non-Patent Citations (3)
| Title |
|---|
| 易磊: "《妙用本草良方》", 31 January 2017, 中医古籍出版社 * |
| 曹翠玲: ""康氏木霉的拮抗机制及发酵条件的初步研究"", 《中国优秀博硕士学位论文全文数据库 (硕士)农业科技辑》 * |
| 陈秀峰等: "《植物病害生物防治》", 31 January 1993, 陕西人民教育出版社 * |
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