CN108977351A - The automatic bacterium solution spreader of use for laboratory - Google Patents
The automatic bacterium solution spreader of use for laboratory Download PDFInfo
- Publication number
- CN108977351A CN108977351A CN201811270548.2A CN201811270548A CN108977351A CN 108977351 A CN108977351 A CN 108977351A CN 201811270548 A CN201811270548 A CN 201811270548A CN 108977351 A CN108977351 A CN 108977351A
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- China
- Prior art keywords
- bacterium solution
- laboratory
- automatic
- culture dish
- rotary motion
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Links
- 241000894006 Bacteria Species 0.000 title claims abstract description 24
- 239000011248 coating agent Substances 0.000 claims abstract description 11
- 238000000576 coating method Methods 0.000 claims abstract description 11
- 239000011521 glass Substances 0.000 claims abstract description 11
- 229920001971 elastomer Polymers 0.000 claims description 4
- 229920001817 Agar Polymers 0.000 claims 1
- 239000008272 agar Substances 0.000 claims 1
- 239000000806 elastomer Substances 0.000 claims 1
- 238000013461 design Methods 0.000 abstract description 6
- 238000010422 painting Methods 0.000 abstract description 2
- 239000000243 solution Substances 0.000 description 9
- 238000000034 method Methods 0.000 description 8
- 238000002474 experimental method Methods 0.000 description 3
- 238000012360 testing method Methods 0.000 description 3
- 239000012895 dilution Substances 0.000 description 2
- 238000010790 dilution Methods 0.000 description 2
- 238000011081 inoculation Methods 0.000 description 2
- 239000007788 liquid Substances 0.000 description 2
- 238000007747 plating Methods 0.000 description 2
- 238000010586 diagram Methods 0.000 description 1
- 238000009792 diffusion process Methods 0.000 description 1
- 239000004744 fabric Substances 0.000 description 1
- 238000011173 large scale experimental method Methods 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- 239000000463 material Substances 0.000 description 1
- 230000002906 microbiologic effect Effects 0.000 description 1
- 244000005700 microbiome Species 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 238000011160 research Methods 0.000 description 1
- 230000000630 rising effect Effects 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M33/00—Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus
- C12M33/02—Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus by impregnation, e.g. using swabs or loops
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Organic Chemistry (AREA)
- Engineering & Computer Science (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Chemical & Material Sciences (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Sustainable Development (AREA)
- Microbiology (AREA)
- Biotechnology (AREA)
- Biomedical Technology (AREA)
- Biochemistry (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Molecular Biology (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
Abstract
This project, which essentially consists in, designs a kind of automatic high-performance bacterium solution spreader of use for laboratory, the painting bacterium device is mainly characterized by using a power source while realizing the straight reciprocating motion of glass bar and the rotary motion of culture dish, speed cooperation is reasonable, it is precisely accomplished the operation of automatic coating, structure is simple, exquisite small and exquisite, easy to operate, cost is relatively low, is suitble to middle-size and small-size laboratory.
Description
Technical field
The present invention realizes bacterium solution automatic coating using mechanical structure cooperation motor.
Background technique
It is well known that requiring to carry out this link of microorganism count, most often at present in most of microbiological manipulations
Mode is exactly dilution plate counting method.Bacterium solution coating is the basic operation in dilution plate counting method, it is due to being experiment
Personnel's manual operations, easy to operate, feasibility is strong, highly reliable, therefore is used for many years.But traditional manual operations is also sudden and violent
Expose many problems, such as: operation low efficiency loaded down with trivial details, time-consuming, hand-manipulated, and cannot be guaranteed the uniformity for applying plate, one
It is secondary can only a plate, delay the process of experiment significantly, influence experiment accuracy the problems such as.Manual operations is suitable for small
The work of batch needs to arrange a large amount of manpower and material resources and time if a large amount of plate coating works need disposable completion
It completes, it is time-consuming and laborious.Therefore replace manual operations with machine, improve working efficiency, the automatic design for applying plate machinery is just aobvious
It obtains particularly important.
It is external at present that there are also automatic bacterium solution spreaders.As manufactured and designed by France/bioMerieux (Mei Liai)
Automatically plating instrument and the DW-16 type automatic bacteria coating inoculation instrument by Hangzhou design and manufacture micro- greatly.Although they can be compared with
It solves the problems, such as well to apply the cumbersome and time-consuming of bacterium process.But full-automatic plating instrument figure is larger, takes up space big, is suitable for big
In batches, the large-scale experiment room of more bacterium colonies research, required specimen amount is big in experimentation, and use process power consumption is big, and
Price is prohibitively expensive for middle-size and small-size laboratory;And DW-16 type automatic bacteria coating inoculation instrument appoint need manual operations into
Row auxiliary, generally still needs to go to apply one by one, is not obviously improved working efficiency.Sample is in culture dish rotation simultaneously
When diffusion, the probability that Specimen liquid is accumulated in culture dish edge corner is increased, the process counted for bacterium colony increases difficulty, most
Cause the influence of experimental data not applicable eventually and middle-size and small-size laboratory.Therefore there is a problem that can be with without handy, practicability
It is not high.
Summary of the invention
A kind of light and handy simple, automatic bacterium solution spreader towards laboratory operation of major design of the present invention.The bacterium solution applies
Cloth device is mainly characterized by through a motor output rotary motion, at the same realize four culture dishes in chassis rotary motion and
The straight reciprocating motion of glass bar, to be automatically performed the even spread of bacterium solution.The design is to reduce labor intensity and mention
Rising and applying bacterium efficiency is target, and design principle is the straight line for the rotary motion of motor being become using class slider-crank mechanism glass bar
It moves back and forth, while the rotary motion of main shaft is transmitted on chassis using four gears, realize the rotary motion of culture dish.It is whole
A mechanism only needs a power source, and structure is simple, and exquisite small and exquisite, easy to operate, cost is relatively low, is suitble to middle-size and small-size laboratory.
The features of the present invention and purposes: this bacterium solution spreader realizes bacterium using simple class slider-crank mechanism and gear
The problem of coating of liquid, preferably resolving middle-size and small-size laboratory painting bacterium, manually time-consuming, cumbersome and expensive equipment.
Detailed description of the invention:
Fig. 1 is bacterium solution spreader complete machine schematic diagram
Specific embodiment
Below in conjunction with attached drawing, the present invention is described in further detail.
Specific operating process is as follows:
Motor [1] exports rotary motion, driving crank [2] movement, and crank [2] is changed into rotary motion directly by connecting rod [3]
The straight reciprocating motion of line guide rail [4] is fixed glass rod connecting rod [5] in linear guide [4], and glass bar [6] is connected by rubber tube [7]
On glass rod connecting rod [5], to realize the linear reciprocation coating movement of glass bar.Crank [2] lower end is connected to bottom of device
On gear wheel [8], motion homogeneous is distributed to four pinion gears [9] being engaged with, and drives the culture dish pallet being connected thereon
[10], the low speed rotation of culture dish is realized, it is final to realize automatic coating operation.
For the above tests novel actual implementation according to this, through the above description, correlation test personnel
Various various forms of changes can be made in the case where not departing from the novel spirit and scope of this test completely.It is all without prejudice to the present invention
The various modifications or variation that spirit is engaged in, belong to the scope this invention is intended to protection.
Claims (3)
1. a kind of automatic bacterium solution spreader of use for laboratory, including motor [1], crank [2], connecting rod [3], linear guide [4], glass
Stick connecting rod [5], rubber tube [7], gear set [8,9], culture dish pallet [10], which is characterized in that utilize class slider-crank mechanism
The rotary motion of motor is become the straight reciprocating motion of glass bar, while the rotary motion of main shaft is transmitted using four gears
Onto culture dish pallet, the rotary motion of culture dish is realized, complete the automatic coating of bacterium solution.
2. the automatic bacterium solution spreader of use for laboratory as described in claim 1, which is characterized in that only with a rotary motive power source,
Realize two different motion modes simultaneously, the rotary motion of straight reciprocating motion and culture dish including glass bar, and protect
Suitable speed proportional is held, there is even spread effect identical with manual application.
3. the automatic bacterium solution spreader of use for laboratory as described in claim 1, which is characterized in that carry out glass bar with rubber tube
Connection, it is easy to disassemble and install, while guaranteeing integrality of the agar in coating using elastomer.
Priority Applications (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
CN201811270548.2A CN108977351B (en) | 2018-10-29 | 2018-10-29 | Automatic bacterial liquid coater for laboratory |
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
CN201811270548.2A CN108977351B (en) | 2018-10-29 | 2018-10-29 | Automatic bacterial liquid coater for laboratory |
Publications (2)
Publication Number | Publication Date |
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CN108977351A true CN108977351A (en) | 2018-12-11 |
CN108977351B CN108977351B (en) | 2024-05-03 |
Family
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Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
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CN201811270548.2A Active CN108977351B (en) | 2018-10-29 | 2018-10-29 | Automatic bacterial liquid coater for laboratory |
Country Status (1)
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CN (1) | CN108977351B (en) |
Cited By (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
WO2024108395A1 (en) * | 2022-11-22 | 2024-05-30 | 深圳先进技术研究院 | Microbial liquid coating device and coating method therefor |
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US3962040A (en) * | 1974-03-14 | 1976-06-08 | The United States Of America As Represented By The Department Of Health, Education And Welfare | Method and apparatus for plating and counting aerobic bacteria |
JPH09187268A (en) * | 1996-01-11 | 1997-07-22 | Nishi Nippon Shinku Tank Kk | Device for analyzing bacteria |
CN2673078Y (en) * | 2003-10-24 | 2005-01-26 | 连云港国鑫医药设备有限公司 | Liquid bacterial culture inoculating device |
FR2876385A1 (en) * | 2004-10-12 | 2006-04-14 | Interscience Sarl | Inoculator for depositing biological solutions in a culture medium comprises distribution of the solution by using an inoculating arm with a tubular element/stylet that delivers the solution above the medium recipient |
CN104152348A (en) * | 2014-07-16 | 2014-11-19 | 北京赛智创业科技有限公司 | Rotary coating device and coating rod |
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CN205398628U (en) * | 2016-03-01 | 2016-07-27 | 新奥科技发展有限公司 | A vaccinate device automatically for declining algae solid culture |
CN105886383A (en) * | 2016-06-14 | 2016-08-24 | 重庆庞通医疗器械有限公司 | Microorganism streak inoculation instrument |
CN205687934U (en) * | 2016-06-14 | 2016-11-16 | 重庆庞通医疗器械有限公司 | Gear driven type microorganism streak inoculation instrument |
CN206266554U (en) * | 2016-12-06 | 2017-06-20 | 邯郸学院 | A kind of microbial inoculant device of knee-piece |
CN206591120U (en) * | 2017-03-13 | 2017-10-27 | 苏州金唯智生物科技有限公司 | A kind of apparatus for coating |
CN109694818A (en) * | 2017-10-23 | 2019-04-30 | 四川农业大学 | A kind of microculture plate apparatus for coating |
CN209049287U (en) * | 2018-10-19 | 2019-07-02 | 高鸿章 | A kind of medical microbial dilution mixing device |
CN209481670U (en) * | 2018-10-29 | 2019-10-11 | 西北农林科技大学 | The automatic bacterium solution spreader of use for laboratory |
CN214612430U (en) * | 2021-04-20 | 2021-11-05 | 西安文理学院 | A simple and easy board auxiliary device that scribbles for molecular cloning experiment |
CN216274105U (en) * | 2021-08-02 | 2022-04-12 | 西北农林科技大学 | Small semi-automatic bacteria liquid coating machine |
-
2018
- 2018-10-29 CN CN201811270548.2A patent/CN108977351B/en active Active
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---|---|---|---|---|
US3962040A (en) * | 1974-03-14 | 1976-06-08 | The United States Of America As Represented By The Department Of Health, Education And Welfare | Method and apparatus for plating and counting aerobic bacteria |
JPH09187268A (en) * | 1996-01-11 | 1997-07-22 | Nishi Nippon Shinku Tank Kk | Device for analyzing bacteria |
CN2673078Y (en) * | 2003-10-24 | 2005-01-26 | 连云港国鑫医药设备有限公司 | Liquid bacterial culture inoculating device |
FR2876385A1 (en) * | 2004-10-12 | 2006-04-14 | Interscience Sarl | Inoculator for depositing biological solutions in a culture medium comprises distribution of the solution by using an inoculating arm with a tubular element/stylet that delivers the solution above the medium recipient |
CN104152348A (en) * | 2014-07-16 | 2014-11-19 | 北京赛智创业科技有限公司 | Rotary coating device and coating rod |
CN204052053U (en) * | 2014-07-16 | 2014-12-31 | 北京赛智创业科技有限公司 | A kind of rotary coating device and pallet |
CN205398628U (en) * | 2016-03-01 | 2016-07-27 | 新奥科技发展有限公司 | A vaccinate device automatically for declining algae solid culture |
CN105886383A (en) * | 2016-06-14 | 2016-08-24 | 重庆庞通医疗器械有限公司 | Microorganism streak inoculation instrument |
CN205687934U (en) * | 2016-06-14 | 2016-11-16 | 重庆庞通医疗器械有限公司 | Gear driven type microorganism streak inoculation instrument |
CN206266554U (en) * | 2016-12-06 | 2017-06-20 | 邯郸学院 | A kind of microbial inoculant device of knee-piece |
CN206591120U (en) * | 2017-03-13 | 2017-10-27 | 苏州金唯智生物科技有限公司 | A kind of apparatus for coating |
CN109694818A (en) * | 2017-10-23 | 2019-04-30 | 四川农业大学 | A kind of microculture plate apparatus for coating |
CN209049287U (en) * | 2018-10-19 | 2019-07-02 | 高鸿章 | A kind of medical microbial dilution mixing device |
CN209481670U (en) * | 2018-10-29 | 2019-10-11 | 西北农林科技大学 | The automatic bacterium solution spreader of use for laboratory |
CN214612430U (en) * | 2021-04-20 | 2021-11-05 | 西安文理学院 | A simple and easy board auxiliary device that scribbles for molecular cloning experiment |
CN216274105U (en) * | 2021-08-02 | 2022-04-12 | 西北农林科技大学 | Small semi-automatic bacteria liquid coating machine |
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Publication number | Priority date | Publication date | Assignee | Title |
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WO2024108395A1 (en) * | 2022-11-22 | 2024-05-30 | 深圳先进技术研究院 | Microbial liquid coating device and coating method therefor |
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CN108977351B (en) | 2024-05-03 |
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