CN104152348A - Rotary coating device and coating rod - Google Patents
Rotary coating device and coating rod Download PDFInfo
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- CN104152348A CN104152348A CN201410339182.5A CN201410339182A CN104152348A CN 104152348 A CN104152348 A CN 104152348A CN 201410339182 A CN201410339182 A CN 201410339182A CN 104152348 A CN104152348 A CN 104152348A
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- coating
- inboardend
- spreading rod
- culture dish
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M33/00—Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus
- C12M33/02—Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus by impregnation, e.g. using swabs or loops
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Abstract
The invention relates to the technical field of microbial machinery and discloses a rotary coating device and a coating rod. The rotary coating device comprises a motor, a tray connected with an output shaft of the motor, a fixing mechanism and a coating rod, wherein the tray is used for fixing a culture dish and is horizontally arranged; the coating rod comprises a fixing end, a coating end, and a connecting handle connected with the fixing end and the coating end; the fixing end is fixed on the fixing mechanism; the coating end stretches into the culture dish. With the adoption of the technical scheme, the motor can drive the tray to horizontally rotate, the fixing end of the coating rod is fixed on the fixing mechanism, the coating end of the coating rod stretches into the culture dish, and when microbial coating is needed to be performed, a tester only needs to start the motor, the motor drives the tray to rotate, the coating rod coats bacteria solution on a culture medium in the culture dish, automatic microbial coating can be realized, and the coating efficiency is greatly improved.
Description
Technical field
The present invention relates to microorganism technique with the apparatus field, particularly relate to a kind of rotary coating device and a kind of spreading rod.
Background technology
Coating method inoculation is a kind of conventional microbial culture method, not only can, for microorganism count with separated, can also utilize its feature that forms bacterium colony in planar surface growth to observe microorganism.In traditional microorganism culturing technology, microorganism coating manipulation require first carries out a series of gradient dilution by bacterium liquid, the more dilution bacterium liquid of difference is applied to respectively to the surface of Solid agar culture, in coating operation, needs to use culture dish and spreading rod.As shown in Fig. 1 a and Fig. 1 b, the structure of existing two kinds of spreading rods, the structure that existing spreading rod is conventional is that handheld terminal (as Fig. 1 a and Fig. 1 b top) is linear structure, coating end (as Fig. 1 a and Fig. 1 b bottom) is equilateral triangle or Alignment Design.During concrete use, operator on the substratum of culture dish, is coated with appropriate bacterium drop back and forth bacterium liquid with the spreading rod of above-mentioned two kinds of structures and makes it be coated on media surface.
The defect of prior art is, whole coating is operating as manual operation, and human cost is higher, and efficiency is lower; And, after the manual operation time is long, easily cause trier's arm tingle, and then cause crawling even, finally cause test-results inaccurate.
Summary of the invention
The object of this invention is to provide a kind of rotary coating device and a kind of spreading rod, in order to improve microorganism coating efficiency, and improve coating homogeneity.
First the embodiment of the present invention provides a kind of rotary coating device, and described rotary coating device, for the coating operation of microorganism culturing, comprising:
Motor;
With the pallet that the output shaft of described motor is connected, described pallet is for fixing culture dish and be horizontally disposed with;
Fixed mechanism;
Spreading rod, described spreading rod comprises inboardend, coating end and the connection handle that connects described inboardend and described coating end, and wherein, described inboardend is fixed on described fixed mechanism, and described coating end stretches in described culture dish.
In technical solution of the present invention, the rotary coating device that a kind of microorganism culturing is used has been proposed, motor in this rotary coating device can horizontally rotate by driving tray, the inboardend of spreading rod is fixed on fixed mechanism, the coating end of spreading rod stretches in culture dish, when needs carry out microorganism coating, trier only needs to open motor, driven by motor pallet rotation, spreading rod is coated with the bacterium liquid on substratum in culture dish, can realize the automatic coating of microorganism, greatly improved coating efficiency, in addition, due to the rotation of culture dish automatic uniform speed, trier does not need the coating back and forth on substratum of hand-held spreading rod, therefore, also improved the homogeneity of coating.Spreading rod comprises inboardend, coating end and is connected and fixed to be held and is coated with the connection handle of holding, and spreading rod is similar to the spreading rod of I shape, has realized the application of spreading rod in rotary coating device.
Preferably, described coating end is yi word pattern coating end, and described inboardend is yi word pattern inboardend, and described connection handle is to have the connection handle of setting angle, and the opening of described angle is towards described culture dish.
If connection handle is linear connection handle, the culture dish that easily collides with, adopts the connection handle of fold-line-shaped can prevent connection handle collision culture dish in pallet rotary course.
Preferably, the angle of described angle is 110 °~150 °.More excellent, described angle is 129 °.
Preferably, word two ends of described yi word pattern coating end are hemisphere face structure.
Because substratum quality is softer, easy applied rod scratches, and therefore, word two ends of yi word pattern coating end adopt hemisphere face structure design, can effectively prevent that spreading rod from damaging substratum.
Preferably, the length of described yi word pattern coating end is 95%~99% of described culture dish internal diameter.
Existing two kinds of spreading rods, the length of holding with respect to its coating of internal diameter of culture dish is shorter, therefore its space availability ratio is low, coating scope is little, need to repeat for more time coating could be coated on bacterium liquid on substratum, and can cause culture dish bacterium liquid crawling even, impact experiment accuracy, and the yi word pattern of spreading rod coating end length is slightly less than culture dish internal diameter (length of in-line coating end be culture dish internal diameter 95%~99%) in the present invention, during culture dish rotation, the coating end of spreading rod can be coated with whole media surface, coating scope is large, avoided causing the high problem of substratum edge section bacterial concentration due to the effect of centrifugal force, therefore, further improve the homogeneity of coating, and then the accuracy of raising test.
The structure of spreading rod of the present invention is not limit, as long as meet I-shaped structure, for example, described inboardend is trilateral or square; Described coating end is trilateral or square.
Preferably, in described pallet, offer at least the fixedly step trough of culture dish of one-level.
The culture dish of every grade of corresponding a kind of model of step trough, has multistage step trough in pallet, in the situation that not changing pallet, can realize the coating to Multiple Type culture dish, has increased the practicality of pallet.
For above-mentioned any rotary coating device, preferred, described fixed mechanism comprises:
Column;
Be fixed on the permanent seat of described column;
Anchor, comprises connection section and fixed part, and described connection section is articulated with on described permanent seat, and described fixed part is fixed described spreading rod;
Resilient snubber, is arranged between described permanent seat and described connection section, connection section and permanent seat described in the resilient support of described resilient snubber.
In preparation process, may there is the situation of variable thickness in substratum, to automatic coating, cause certain difficulty, in this preferred technical scheme, because this fixed mechanism has resilient snubber, resilient snubber can support connection section and permanent seat, can be under the elastic force effect of resilient snubber, the fixed part that is fixed with spreading rod can be finely tuned with respect to the height of permanent seat, therefore, adopt this device, can automatically adjust the height of spreading rod, overcome the automatic coating difficulty that substratum variable thickness causes.
The embodiment of the present invention also provides a kind of spreading rod, and described spreading rod is for the coating operation of microorganism culturing, and described spreading rod comprises inboardend, coating end and the connection handle that connects described inboardend and described coating end.
Spreading rod comprises inboardend, coating end and is connected and fixed end and the connection handle of coating end, it is the spreading rod that spreading rod is similar to I shape, realized the application of spreading rod in rotary coating device, and then make automatic operation replace manual operation, improved coating efficiency, in addition, due to rotary coating device uniform rotation, with respect to manual operation, improved the homogeneity of coating.
Preferably, described coating end is yi word pattern coating end, and described inboardend is yi word pattern inboardend, and described connection handle is to have the connection handle of setting angle.
If connection handle is linear connection handle, the culture dish that easily collides with, adopts the connection handle of fold-line-shaped can prevent connection handle collision culture dish in pallet rotary course.
Accompanying drawing explanation
Fig. 1 a is the structural representation of existing a kind of spreading rod;
Fig. 1 b is the structural representation of existing another kind of spreading rod;
The structural representation of the rotary coating instrument that Fig. 2 provides for one embodiment of the invention;
Fig. 3 is the perspective view of the spreading rod of the rotary coating device shown in Fig. 2;
Fig. 4 is the main TV structure schematic diagram of the spreading rod shown in Fig. 3;
The structural representation of the fixed mechanism of the rotary coating device that Fig. 5 provides for one embodiment of the invention;
Fig. 6 is the structural representation that comprises the rotary coating device of the fixed mechanism shown in Fig. 5.
Reference numeral:
1-motor 2-pallet 3-culture dish 4-fixed mechanism 5-spreading rod
11-output shaft 41-column 42-permanent seat 43-anchor 44-resilient snubber
45-locking cap 51-inboardend 52-coating end 53-connection handle 431-connection section
432-fixed part
Embodiment
In order to improve microorganism coating efficiency, the invention provides a kind of rotary coating device and a kind of spreading rod.In this technical scheme, motor can horizontally rotate by driving tray, the inboardend of spreading rod is fixed on fixed mechanism, the coating end of spreading rod stretches in culture dish, when needs carry out microorganism coating, trier only needs to open motor, driven by motor pallet rotation, spreading rod is coated with the bacterium liquid on substratum in culture dish, can realize the automatic coating of microorganism, greatly improved coating efficiency, in addition, due to the rotation of culture dish automatic uniform speed, trier does not need the coating back and forth on substratum of hand-held spreading rod, therefore, also improved the homogeneity of coating.For making the object, technical solutions and advantages of the present invention clearer, below lift specific embodiment the present invention is described in further detail.
First the embodiment of the present invention provides a kind of rotary coating device, as shown in Figure 2, the structural representation of the rotary coating instrument that Fig. 2 provides for one embodiment of the invention, described rotary coating device, for the coating operation of microorganism culturing, comprising:
Motor 1;
With the pallet 2 that the output shaft 11 of motor 1 is connected, pallet 2 is for fixing culture dish 3 and be horizontally disposed with;
Fixed mechanism 4;
Spreading rod 5, spreading rod 5 comprises inboardend 51, is coated with end 52 and is connected and fixed end 51 and the connection handle 53 of coating end 52, and wherein, inboardend 51 is fixed on fixed mechanism 4, is coated with end 52 and stretches in culture dish 3.
In technical solution of the present invention, the rotary coating device that a kind of microorganism culturing is used has been proposed, motor 1 in this rotary coating device can horizontally rotate by driving tray 2, the inboardend 51 of spreading rod 5 is fixed on fixed mechanism 4, the coating end 52 of spreading rod 5 stretches in culture dish 3, when needs carry out microorganism coating, trier only needs to open motor 1, motor 1 drives pallet 2 rotations, spreading rod 5 is coated with the bacterium liquid on substratum in culture dish, can realize the automatic coating of microorganism, greatly improved coating efficiency, in addition, due to the rotation of culture dish automatic uniform speed, trier does not need the coating back and forth on substratum of hand-held spreading rod, therefore, also improved the homogeneity of coating.Spreading rod comprises inboardend 51, coating end 52 and is connected and fixed end 51 and the connection handle 53 of coating end 52, it is the spreading rod that spreading rod 5 is similar to I shape, realized the application of spreading rod in rotary coating device, inboardend 51 can be directly fixed on fixed mechanism, without adjusting position.After being coated with, spreading rod 5 is removed to sterilization, in order to other coated test, use, spreading rod is had can reusing.
In technical solution of the present invention, the output shaft 11 of motor 1 can be connected with pallet 2 indirectly, as long as motor 1 can rotate by driving tray 2, for example, the output shaft 11 of motor 1 can pass through shaft coupling, then is connected with pallet 2 by a union lever.
In technical solution of the present invention, can also comprise shell, this shell is used for holding motor and electric wire etc., and the button of the rotating speed that regulates motor etc. also can be set on this shell.In addition, pallet 2 can be placed horizontally on this shell.
As shown in Figure 3 and Figure 4, Fig. 3 is the perspective view of the spreading rod of the rotary coating device shown in Fig. 2, Fig. 4 is the main TV structure schematic diagram of the spreading rod shown in Fig. 3, preferably, coating end 52 is yi word pattern coating end, inboardend 51 is yi word pattern inboardend, and connection handle 53 is for having the connection handle of setting angle, and the opening of angle is towards culture dish.
If connection handle 53 is linear connection handle, the culture dish that easily collides with, adopts the connection handle 53 of fold-line-shaped can prevent connection handle 53 collision culture dish in pallet 2 rotary courses.Preferred, connection handle 53 lays respectively at the mid-way of yi word pattern coating end and yi word pattern inboardend, and symmetrical I-shaped structure, conveniently realizes location automatically.
Shown in Fig. 4, preferred, the angle of angle γ is 110 °~150 °, and preferably, the angle of angle γ is 120 °~130 °, more excellent, and angle γ is 129 °.The shaking degree that the angle of angle γ can be rotated according to the pallet of rotary coating device and the uneven degree of the height of substratum design, and the invention is not restricted to concrete numerical value.But in technical solution of the present invention, through test many times, show that angle γ is preferably at 110 °~150 °, can effectively prevent colliding with of itself and culture dish.
Shown in Fig. 3, preferred, word two ends of yi word pattern coating end are hemisphere face structure.
Because substratum quality is softer, easy applied rod scratches, and therefore, word two ends of yi word pattern coating end adopt hemisphere face structure design, can effectively prevent that spreading rod from damaging substratum.
Preferably, the length of yi word pattern coating end is 95%~99% of culture dish internal diameter, for example, be 95%, 96%, 97%, 98% or 99%.
Existing two kinds of spreading rods, the length of holding with respect to its coating of internal diameter of culture dish is shorter, therefore its space availability ratio is low, coating scope is little, need to repeat for more time coating could be coated on bacterium liquid on substratum, and can cause culture dish bacterium liquid crawling even, impact experiment accuracy, and the yi word pattern of spreading rod coating end length is slightly less than culture dish internal diameter (length of in-line coating end be culture dish internal diameter 95%~99%) in the present invention, during culture dish rotation, the coating end of spreading rod can be coated with whole media surface, coating scope is large, avoided causing the high problem of substratum edge section bacterial concentration due to the effect of centrifugal force, therefore, further improve the homogeneity of coating, and then the accuracy of raising test.
The structure of spreading rod of the present invention is not limit, as long as meet I-shaped structure, for example, described inboardend is trilateral or square; Described coating end is trilateral or square.Spreading rod does not adopt the shaft-like of yi word pattern, and adopt planar, preferred, leg-of-mutton center during with rotary coating the rotation center of culture dish consistent, square center is also preferably consistent with the rotation center of culture dish, can when rotary coating, improve the stationarity of spreading rod like this.
Shown in Fig. 2, preferred, in pallet 2, offer at least the fixedly step trough of culture dish 3 of one-level.
The culture dish of every grade of corresponding a kind of model of step trough, has multistage step trough in pallet, in the situation that not changing pallet, can realize the coating to Multiple Type culture dish, has increased the practicality of pallet.
For above-mentioned any rotary coating device, as shown in Figure 5, the structural representation of the fixed mechanism of the rotary coating device that Fig. 5 provides for one embodiment of the invention, preferred, described fixed mechanism comprises:
Column 41;
Be fixed on the permanent seat 42 of column 41;
Anchor 43, comprises connection section 431 and fixed part 432, and connection section 431 is articulated with on permanent seat 42, and fixed part 432 is spreading rod fixedly;
Resilient snubber 44, is arranged between permanent seat 42 and connection section 431 the resilient support connection section 431 of resilient snubber 44 and permanent seat 42.
In preparation process, may there is the situation of variable thickness in substratum, to automatic coating, cause certain difficulty, in this preferred technical scheme, because this fixed mechanism has resilient snubber 44, resilient snubber 44 can support connection section 431 and permanent seat 42, can be under the elastic force effect of resilient snubber 44, the fixed part 432 that is fixed with spreading rod can be finely tuned with respect to the height of permanent seat 42, therefore, adopt this device, can automatically adjust the height of spreading rod, overcome the automatic coating difficulty that substratum variable thickness causes.Resilient snubber 44 can be spring etc.
As shown in Figure 6, Fig. 6 is the structural representation that comprises the rotary coating device of the fixed mechanism shown in Fig. 5, for a preferred embodiment of the present invention, the fixed part 432 of fixed mechanism is fixed in spreading rod 5 one end, preferably adopts the form of draw-in groove to spreading rod 5 engagings, the coating end of spreading rod 5 is positioned at culture dish 3, because fixed mechanism can be finely tuned up and down, therefore, when coating, the height of spreading rod 5 can be adjusted automatically, has overcome the automatic coating difficulty that substratum variable thickness causes.
Preferably, on described permanent seat 42, be provided with the through hole of sheathed column 41; Described fixed mechanism also comprises:
Locking cap 45, the seat 42 that is locked is in the desired location of column 41.
In this embodiment, adopt locking cap 45, can conveniently regulate the position of permanent seat 42 on column 41, the height that makes to be fixed on the spreading rod 5 on permanent seat 42 can regulate along the height of column 41.
Shown in Fig. 3, the embodiment of the present invention also provides a kind of spreading rod, and described spreading rod is for the coating operation of microorganism culturing, and described spreading rod comprises inboardend 51, coating end 52 and is connected and fixed end 51 and the connection handle 53 of coating end 52.
Spreading rod comprises inboardend 51, coating end 52 and is connected and fixed end 51 and the connection handle 53 of coating end 52, it is the spreading rod that spreading rod is similar to I shape, realized the application of spreading rod in rotary coating device, and then make automatic operation replace manual operation, improved coating efficiency, in addition, due to rotary coating device uniform rotation, with respect to manual operation, improved the homogeneity of coating.
Shown in Fig. 3, preferred, coating end 52 is yi word pattern coating end, and inboardend 51 is yi word pattern inboardend, and connection handle 53 is for having the connection handle of setting angle.
If connection handle is linear connection handle, the culture dish that easily collides with, adopts the connection handle of fold-line-shaped can prevent connection handle collision culture dish in pallet rotary course.
The invention provides a kind of rotary coating device and a kind of spreading rod, spreading rod adopts I shape design, can be applicable in rotary coating device, realizes location automatically, and surface covered is maximized, and then has improved coating efficiency.
Obviously, those skilled in the art can carry out various changes and modification and not depart from the spirit and scope of the present invention the present invention.Like this, if within of the present invention these are revised and modification belongs to the scope of the claims in the present invention and equivalent technologies thereof, the present invention is also intended to comprise these changes and modification interior.
Claims (10)
1. a rotary coating device, is characterized in that, described rotary coating device, for the coating operation of microorganism culturing, comprising:
Motor;
With the pallet that the output shaft of described motor is connected, described pallet is for fixing culture dish and be horizontally disposed with;
Fixed mechanism;
Spreading rod, described spreading rod comprises inboardend, coating end and the connection handle that connects described inboardend and described coating end, and wherein, described inboardend is fixed on described fixed mechanism, and described coating end stretches in described culture dish.
2. rotary coating device as claimed in claim 1, is characterized in that, described coating end is yi word pattern coating end, and described inboardend is yi word pattern inboardend, and described connection handle is to have the connection handle of setting angle, and the opening of described angle is towards described culture dish.
3. rotary coating device as claimed in claim 2, is characterized in that, the angle of described angle is 110 °~150 °.
4. rotary coating device as claimed in claim 2, is characterized in that, word two ends of described yi word pattern coating end are hemisphere face structure.
5. rotary coating device as claimed in claim 2, is characterized in that, the length of described yi word pattern coating end is 95%~99% of described culture dish internal diameter.
6. rotary coating device as claimed in claim 1, is characterized in that, described inboardend is trilateral or square; Described coating end is trilateral or square.
7. rotary coating device as claimed in claim 1, is characterized in that, offers at least the fixedly step trough of culture dish of one-level in described pallet.
8. the rotary coating device as described in claim 1~7 any one, is characterized in that, described fixed mechanism comprises:
Column;
Be fixed on the permanent seat of described column;
Anchor, comprises connection section and fixed part, and described connection section is articulated with on described permanent seat, and described fixed part is fixed described spreading rod;
Resilient snubber, is arranged between described permanent seat and described connection section, connection section and permanent seat described in the resilient support of described resilient snubber.
9. a spreading rod, is characterized in that, described spreading rod is for the coating operation of microorganism culturing, and described spreading rod comprises inboardend, coating end and the connection handle that connects described inboardend and described coating end.
10. spreading rod as claimed in claim 9, described coating end is yi word pattern coating end, and described inboardend is yi word pattern inboardend, and described connection handle is to have the connection handle of setting angle.
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CN201410339182.5A CN104152348A (en) | 2014-07-16 | 2014-07-16 | Rotary coating device and coating rod |
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CN201410339182.5A CN104152348A (en) | 2014-07-16 | 2014-07-16 | Rotary coating device and coating rod |
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CN201410339182.5A Pending CN104152348A (en) | 2014-07-16 | 2014-07-16 | Rotary coating device and coating rod |
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Cited By (7)
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CN104498349A (en) * | 2014-12-04 | 2015-04-08 | 济南朗迈生物科技有限公司 | Method and device for culture medium inoculation by using gravity to control strength |
CN106893673A (en) * | 2017-04-11 | 2017-06-27 | 尹康康 | A kind of soil bacteria separates counting experimental provision |
CN107216997A (en) * | 2017-07-24 | 2017-09-29 | 东北林业大学 | Bacterium solution apparatus for coating and coating method |
CN108977351A (en) * | 2018-10-29 | 2018-12-11 | 西北农林科技大学 | The automatic bacterium solution spreader of use for laboratory |
CN109251850A (en) * | 2018-11-13 | 2019-01-22 | 无锡市疾病预防控制中心 | A kind of automatic L stick of hand-held |
CN110747114A (en) * | 2019-10-25 | 2020-02-04 | 西北农林科技大学 | Dull and stereotyped coating unit of modularization |
CN113699029A (en) * | 2021-07-21 | 2021-11-26 | 四川若斌生物科技有限责任公司 | Automatic plate coating instrument and automatic plate coating method |
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CN203451528U (en) * | 2013-07-12 | 2014-02-26 | 赵冰 | Bacterium coating stick |
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CN202671538U (en) * | 2012-06-28 | 2013-01-16 | 庄菁 | Bacterium coating rod |
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Cited By (10)
Publication number | Priority date | Publication date | Assignee | Title |
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CN104498349A (en) * | 2014-12-04 | 2015-04-08 | 济南朗迈生物科技有限公司 | Method and device for culture medium inoculation by using gravity to control strength |
CN104498349B (en) * | 2014-12-04 | 2016-05-18 | 济南朗迈生物科技有限公司 | A kind of culture medium inoculated method and apparatus that utilizes GRAVITY CONTROL dynamics |
CN106893673A (en) * | 2017-04-11 | 2017-06-27 | 尹康康 | A kind of soil bacteria separates counting experimental provision |
CN107216997A (en) * | 2017-07-24 | 2017-09-29 | 东北林业大学 | Bacterium solution apparatus for coating and coating method |
CN108977351A (en) * | 2018-10-29 | 2018-12-11 | 西北农林科技大学 | The automatic bacterium solution spreader of use for laboratory |
CN108977351B (en) * | 2018-10-29 | 2024-05-03 | 西北农林科技大学 | Automatic bacterial liquid coater for laboratory |
CN109251850A (en) * | 2018-11-13 | 2019-01-22 | 无锡市疾病预防控制中心 | A kind of automatic L stick of hand-held |
CN110747114A (en) * | 2019-10-25 | 2020-02-04 | 西北农林科技大学 | Dull and stereotyped coating unit of modularization |
CN110747114B (en) * | 2019-10-25 | 2023-04-04 | 西北农林科技大学 | Dull and stereotyped coating unit of modularization |
CN113699029A (en) * | 2021-07-21 | 2021-11-26 | 四川若斌生物科技有限责任公司 | Automatic plate coating instrument and automatic plate coating method |
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Application publication date: 20141119 |