CN108968031A - The antitumor row liver poison Wild ganoderma product of anticancer and technology of preparing - Google Patents
The antitumor row liver poison Wild ganoderma product of anticancer and technology of preparing Download PDFInfo
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- CN108968031A CN108968031A CN201810884840.7A CN201810884840A CN108968031A CN 108968031 A CN108968031 A CN 108968031A CN 201810884840 A CN201810884840 A CN 201810884840A CN 108968031 A CN108968031 A CN 108968031A
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- 241000222336 Ganoderma Species 0.000 title claims abstract description 56
- 210000004185 liver Anatomy 0.000 title claims abstract description 53
- 230000001093 anti-cancer Effects 0.000 title claims abstract description 48
- 230000000259 anti-tumor effect Effects 0.000 title claims abstract description 48
- 239000002574 poison Substances 0.000 title claims abstract description 48
- 231100000614 poison Toxicity 0.000 title claims abstract description 48
- 238000005516 engineering process Methods 0.000 title claims abstract description 25
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- OQMZNAMGEHIHNN-UHFFFAOYSA-N 7-Dehydrostigmasterol Natural products C1C(O)CCC2(C)C(CCC3(C(C(C)C=CC(CC)C(C)C)CCC33)C)C3=CC=C21 OQMZNAMGEHIHNN-UHFFFAOYSA-N 0.000 abstract 1
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- CIWBSHSKHKDKBQ-DUZGATOHSA-N D-isoascorbic acid Chemical compound OC[C@@H](O)[C@H]1OC(=O)C(O)=C1O CIWBSHSKHKDKBQ-DUZGATOHSA-N 0.000 description 1
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Classifications
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L31/00—Edible extracts or preparations of fungi; Preparation or treatment thereof
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23B—PRESERVING, e.g. BY CANNING, MEAT, FISH, EGGS, FRUIT, VEGETABLES, EDIBLE SEEDS; CHEMICAL RIPENING OF FRUIT OR VEGETABLES; THE PRESERVED, RIPENED, OR CANNED PRODUCTS
- A23B7/00—Preservation or chemical ripening of fruit or vegetables
- A23B7/14—Preserving or ripening with chemicals not covered by groups A23B7/08 or A23B7/10
- A23B7/153—Preserving or ripening with chemicals not covered by groups A23B7/08 or A23B7/10 in the form of liquids or solids
- A23B7/154—Organic compounds; Microorganisms; Enzymes
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L21/00—Marmalades, jams, jellies or the like; Products from apiculture; Preparation or treatment thereof
- A23L21/20—Products from apiculture, e.g. royal jelly or pollen; Substitutes therefor
- A23L21/25—Honey; Honey substitutes
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2002/00—Food compositions, function of food ingredients or processes for food or foodstuffs
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- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Polymers & Plastics (AREA)
- Food Science & Technology (AREA)
- Nutrition Science (AREA)
- Health & Medical Sciences (AREA)
- Microbiology (AREA)
- Mycology (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- General Chemical & Material Sciences (AREA)
- Medicines Containing Plant Substances (AREA)
- Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
Abstract
The invention discloses a kind of antitumor row liver poison Wild ganoderma product of anticancer and technologies of preparing.The present invention is using Wild ganoderma as raw material, it is cleaned, sterilizing, it is dry, stripping and slicing and etc. the antitumor row liver poison Wild ganoderma product of anticancer finally prepared, with inhibition growth of cancer cells, treating acute and chronic hepatitis, early-phase hepatocirrhosis, the cancer of the esophagus, lung cancer, liver cancer, nasopharyngeal carcinoma, cancer of pancreas, neurasthenia, intestines wind rushes down blood, rheumatic pulmonary tuberculosis, nourishing and fit keeping function diabetes, it is hypoglycemic, gastrointestinal disease, neurasthenia, moistening lung kidney-nourishing, beautifying face and moistering lotion, enhance immunity of organisms and other effects, ergosterol rich in, ganoderic acid, ganoderma lucidum polysaccharide, amino acid, polypeptide, adenosine, triterpenes and various trace elements, wherein organic germanium content is up to 12380ppm, organic selenium content is also very high.It can be made into multicolored ganoderma tea, Ganoderma lucidum drink, Ganoderma lucidum oral liquid, multicolored Lucid Ganoderma wine, wait integration of drinking and medicinal herbs product, the present invention can also be made to the Chinese medicinal formulaes such as cream, pellet, ball.
Description
Technical field
The present invention relates to field of health care food, and in particular to a kind of antitumor row liver poison Wild ganoderma product of anticancer and preparation
Technology.
Background technique
The people of modern society just by from all directions and come pollution, have quite a few Genus Homo " controlling not in Chinese medicine
" inferior health " scope of disease " or modern medicine, the self-healing ability and immune function dramatic decrease of body.Body is one extremely multiple
Miscellaneous system, especially using the vital organs of the human body as the main force, it is indispensable that Each performs its own functions, and the five internal organs liver storing blood wherein, catharsis are adjustable blood
Flow quantity and whole body mechanism of qi.And when physical activity, blood volume needed for body increases, and blood then can be transported to body by liver and respectively pass through
Arteries and veins, for needed for each histoorgan of whole body.Hepatic injury can directly cause vascular problem and disease at first, and then enable each vital organs of the human body
Body function decline enables body fall into vicious circle, reduces immunity of organisms.Scientific research proves that human body about generates 10,000 daily
A or so cancer cell can normally be tackled by human immune system, and common people will not obtain cancer, but every point of modern humans it is per second by
The ability for directly reducing human immune system and tackling cancer cell is attacked in pollution to cubic octahedral, along with suitable cancer cell becomes
Different acidic constitution will greatly improve and suffer from cancer risk.
Modern seldom contacts Wild ganoderma, and being known by traditional consciousness implantation idea is that Ganoderma is unreachable valuable
Product.In ancient times, it is rare in the depths of the forest to be grown in human footmarks for ganoderma lucidum, very rare, and is unable to artificial cultivation, makes the masses of the people to open country
The understanding of creature's sesame mystery effect is seldom, and can only hope and halt, and fails to have an opportunity really to understand ganoderma lucidum.Ganoderma lucidum has immune adjust
Section, resist oxygen lack, antifatigue health-care effect, application range are very extensive.Ganoderma lucidum enters the five internal organs, the gas of completion body, the heart, lung, liver,
Spleen, kidney weakness can take it.Ganoderma lucidum, which has, strengthens the body resistance to consolidate the constitution, and enhancing is immune, improves the effect of Abwehrkraft des Koepers.It is different from
General therapeutic effect of the drug to certain disease is also different from general nutritional health food only to the deficiency of nutrient in a certain respect
It is supplemented and is strengthened, but bidirectional modulation function of human body balances on the whole, transfers internal body vigor, and it is newly old to adjust human body
Metabolism improves autoimmune ability, promotes whole internal organ or organ function normalization.
Currently, ganoderma lucidum is sweet in flavor and bitter, directly soaked, effective component cannot be fully dissolved out, thus its medicinal efficacy and
Health-care efficacy is difficult to be not fully exerted.
Summary of the invention
In view of the above-mentioned deficiencies in the prior art, the present invention provides a kind of antitumor row liver poison Wild ganodermas of anticancer
Product and technology of preparing.
A kind of technology of preparing of the antitumor row liver poison Wild ganoderma product of anticancer, comprising the following steps:
I, ganoderma applanatum is cleaned, after sterilization processing, is placed at 40-50 DEG C and is dried, stripping and slicing obtains block stock；
II, at 35-60 DEG C to above-mentioned block stock baked to get.
Preferably, the technology of preparing of the antitumor row liver poison Wild ganoderma product of the anticancer, comprising the following steps:
I, ganoderma applanatum is cleaned, after sterilization processing, is placed at 40-50 DEG C and is dried, stripping and slicing obtains block stock；
II, bakes above-mentioned block stock at 35-60 DEG C, the block stock after being baked；
III, mixes honey, antioxidant fresh-keeping agent, extract, water, wherein the honey, antioxidant fresh-keeping agent, extract, water
Mass ratio be (0.1-0.5): (0.005-0.015): (0.1-0.5): (10-20) obtains mixed liquor after mixing；Then 1 is pressed:
Block stock after baking is added in mixed liquor the mass volume ratio of (10-20) (g/mL), is impregnated at 10-20 DEG C,
It drains away the water after taking-up, it is dry, obtain the antitumor row liver poison Wild ganoderma product of anticancer.
It is highly preferred that the technology of preparing of the antitumor row liver poison Wild ganoderma product of anticancer, comprising the following steps:
I, ganoderma applanatum is cleaned, after sterilization processing, is placed at 40-50 DEG C and is dried, stripping and slicing obtains block stock；
II, bakes above-mentioned block stock at 35-60 DEG C, the block stock after being baked；
III, puts into the block stock after baking in means of supercritical extraction equipment, under the carbon dioxide flow of 50-100L/h
Supercritical extract processing is carried out, the block stock of supercritical extract processing is obtained；
IV, mixes honey, antioxidant fresh-keeping agent, extract, water, wherein the honey, antioxidant fresh-keeping agent, extract, water
Mass ratio be (0.1-0.5): (0.005-0.015): (0.1-0.5): (10-20) obtains mixed liquor after mixing；Then 1 is pressed:
The block stock that the mass volume ratio of (10-20) (g/mL) handles supercritical extract is added in mixed liquor, at 10-20 DEG C into
Row impregnates, and drains away the water after taking-up, dry, obtains the antitumor row liver poison Wild ganoderma product of anticancer.
Preferably, the sterilization temperature of the sterilization processing is 100-120 DEG C, sterilizing time 5-10s.
The antioxidant fresh-keeping agent includes one of sodium isoascorbate, Sodium Ascorbyl Phosphate, chitosan or a variety of.
Preferably, the antioxidant fresh-keeping agent presses (1-6) by sodium isoascorbate, Sodium Ascorbyl Phosphate, chitosan:
(1-6): the mass ratio of (1-6) mixes.
The extract includes one of licorice, chrysanthemum extract, dried orange peel extracts or a variety of.
Preferably, the extract presses (1-6) by licorice, chrysanthemum extract, dried orange peel extracts: (1-6): (1-
6) mass ratio mixes.
Preferably, the supercritical extract processing is circulating using two points of an extraction: overcritical during supercritical extract
Carbon dioxide is successively gradually depressured through extraction kettle, knockout drum I, knockout drum II, can make to extract in carbon dioxide gas after decompression
Liposoluble constituent be precipitated from knockout drum, then carbon dioxide gas is boosted again as supercritical carbon dioxide, is made into extractor
It is recycled；Wherein the extracting pressure of extraction kettle is 30-50MPa, and temperature is 40-50 DEG C；The pressure of knockout drum I is 11-
15MPa, temperature are 30-35 DEG C；The pressure of knockout drum II is 5-6MPa, and temperature is 30-35 DEG C.
Garnoderma product prepared by the present invention can with the wild honeycomb bacterium of yellow, the wild ganoderma capense of black, the wild purple sesame of purple,
Multicolored ganoderma tea, Ganoderma lucidum drink, ganoderma lucidum mouthful is made in variegated wild rainbow conk, the wild Inonotus obliquus of yellow, red wild stockless red sesame
The integration of drinking and medicinal herbs products such as liquid, multicolored Lucid Ganoderma wine are taken, the present invention can also be made to the Chinese medicinal formulaes such as cream, pellet, ball.
The technology of preparing of the antitumor row liver poison Wild ganoderma product of anticancer of the present invention can be using Wild ganoderma as raw material, can also
To use non-semi-wild ganoderma lucidum (i.e. artificial field cultivating ganoderma lucidum) as raw material.
By adopting the above-described technical solution, compared with prior art, the present invention has the following advantages: institute of the present invention
The antioxidant fresh-keeping agent of use has good corrosion-resistanting fresh-keeping effect, can prevent the quality of product from occurring during drying and storage
Change；Extract used in the present invention complements each other, synergistic, enhances the antitumor row liver poison Wild ganoderma of anticancer significantly
For cancer, the anticancer function of product；Minerals and enzyme rich in honey, are handled ganoderma lucidum using honey, Ji Nenghuan
The bitter taste of the antitumor row liver poison Wild ganoderma product of anticancer is solved, and its health-care efficacy can be enhanced；In addition, using supercritical extract
Method handles raw material, can preferentially extract liposoluble constituent in raw material from raw material, raw material mix is more loose, expands
Dialysis membrane diameter increases the surface area contacted with water, improves such as dissolution of ganoderma lucidum polysaccharide water-soluble active ingredient in raw material
Rate.The present invention using Wild ganoderma as raw material, cleaned, sterilizing, stripping and slicing and etc. the antitumor row liver poison of anticancer prepared it is wild
Garnoderma product, have inhibit cell carcinoma tumor cell growth, treat B-mode treating acute and chronic hepatitis, early-phase hepatocirrhosis, the cancer of the esophagus,
Lung cancer, liver cancer, nasopharyngeal carcinoma, cancer of pancreas, neurasthenia, intestines wind rush down blood, rheumatic pulmonary tuberculosis, nourishing and fit keeping function diabetes, it is hypoglycemic,
Gastrointestinal disease, neurasthenia, moistening lung kidney-nourishing, beautifying face and moistering lotion, enhancing immunity of organisms and other effects.
Specific embodiment
Foregoing invention content of the invention is described in further detail With reference to embodiment, but should not be incited somebody to action
This range for being interpreted as the above-mentioned theme of the present invention is only limitted to following embodiments.
Each raw material and equipment introduction in embodiment:
Ganoderma applanatum is the fructification of Ganodermataceae Ganoderma ganoderma applanatum, latin name: Ganoderma applanatum
(pers) pat, place of production Hainan are purchased from Anhui Province's Jinzhai County shore Biotechnology Co., Ltd.
Honey, type Mel Jujubae are purchased from Mengyin County Yongxing bee raising Specialty Co-operative Organization
Sodium isoascorbate, No. CAS: 6381-77-7, food-grade is purchased from Henan Xi He Chemical Co., Ltd..
Sodium Ascorbyl Phosphate, No. CAS: 66170-10-3, food-grade pleases the limited public affairs of chemical products purchased from Henan China
Department.
Chitosan, No. CAS: 9012-76-4, food-grade is purchased from the bright and clear Biotechnology Co., Ltd in Xi'an.
Radix Glycyrrhizae, be pulse family Glycyrrhiza perennial herb root, latin name: Glycyrrhiza uralensis Fisch,
Place of production Gansu is purchased from Bozhou often rich medicine company source producer.
Dried orange peel, be rutaceae orange dry mature skin, latin name: Citrus reticulata Blanco,
Pharmaceutcal corporation, Ltd is held court trial purchased from Bozhou City source in place of production Anhui.
Chrysanthemum is the flower of the perennial herb plant of composite family Chrysanthemum, Classification system: Dendranthema
Morifolium (Ramat.) Tzvel., kind FLOS CHRYSANTHEMI ALBA from Haizhou of China are purchased from the source Haitai Ju Ye producer.
Licorice the preparation method comprises the following steps: by Radix Glycyrrhizae clean after naturally dry, be crushed to 100 mesh, obtain Radix Glycyrrhizae powder,
Then 60wt% aqueous acetone solution, the quality volume of the Radix Glycyrrhizae powder and 60wt% aqueous acetone solution are added into Radix Glycyrrhizae powder
Than for 1:8 (g/mL), oscillation under conditions of 50 DEG C, 200r/min extracts 2h, through 200 mesh filter-cloth filterings, obtain liquor C and
Radix Glycyrrhizae filter residue；Gained dried orange peel filter residue adds 60wt% ethanol water, the dried orange peel filter residue and 60wt% ethanol water
Mass volume ratio is 1:8 (g/mL), and oscillation extraction 2h under conditions of 50 DEG C, 200r/min is obtained through 200 mesh filter-cloth filterings
To filtrate D, merging filtrate C and filtrate D, gained mixed extract is at subzero 2 DEG C with the revolving speed refrigerated centrifuge of 15000r/m
28min, gained supernatant is placed in 55 DEG C, spin concentration in the rotary evaporation instrument of vacuum degree 0.05MPa, until residual volume is
Until the 4% of original volume, licorice is obtained.
The preparation method of dried orange peel extracts and the preparation method of licorice are identical, and Radix Glycyrrhizae is only replaced with dried orange peel.
Chrysanthemum extract dries 3h the preparation method comprises the following steps: chrysanthemum is placed at 50 DEG C, is crushed to 40 meshes and weighs by 1:4
(g/mL) ethanol water of 80wt% is added in solid-liquid ratio, extracts 3h at 85 DEG C, through 150 mesh filter-cloth filterings, gained filtrate is set
In the Rotary Evaporators that temperature is 55 DEG C, absolute pressure is 0.05MPa be concentrated into water content be 5% to get.
Means of supercritical extraction equipment is HA121-50-01-C type supercritical extraction unit (using two split flows of extraction), is purchased from
Jiangsu Province Nantong Huaan supercritical extract Co., Ltd.
Embodiment 1
A kind of technology of preparing of the antitumor row liver poison Wild ganoderma product of anticancer, comprising the following steps:
It after I, cleans ganoderma applanatum, is placed at 110 DEG C and sterilizes 6s, then in 45 DEG C of dry 3h, then be cut into long 2cm, width
The fritter of 1cm, thickness 0.5cm, obtain block stock；
II, by above-mentioned block stock be placed at 50 DEG C bake 1.5h to get.
Embodiment 2
A kind of technology of preparing of the antitumor row liver poison Wild ganoderma product of anticancer, comprising the following steps:
It after I, cleans ganoderma applanatum, is placed at 110 DEG C and sterilizes 6s, then in 45 DEG C of dry 3h, then be cut into long 2cm, width
The fritter of 1cm, thickness 0.5cm, obtain block stock；Above-mentioned block stock is placed at 50 DEG C and bakes 1.5h by II, after obtaining baking
Block stock；
III, mixes honey, sodium isoascorbate, Sodium Ascorbyl Phosphate, chitosan, extract, water, wherein described
Honey, sodium isoascorbate, Sodium Ascorbyl Phosphate, chitosan, extract, water mass ratio be 0.2:0.002:0.002:
0.002:0.3:18 stirs 1h at 20 DEG C with the revolving speed of 800r/min, obtains mixed liquor；Then the quality of 1:18 (g/mL) is pressed
Block stock after baking is added in mixed liquor volume ratio, and 10h is impregnated at 15 DEG C and is taken out, 15min is stood, after draining away the water
It is placed at 45 DEG C dry 6h again, obtains the antitumor row liver poison Wild ganoderma product of anticancer.
The extract is mixed by licorice, chrysanthemum extract, dried orange peel extracts by the mass ratio of 1:1:1.
Embodiment 3
A kind of technology of preparing of the antitumor row liver poison Wild ganoderma product of anticancer, comprising the following steps:
It after I, cleans ganoderma applanatum, is placed at 110 DEG C and sterilizes 6s, then in 45 DEG C of dry 3h, then be cut into long 2cm, width
The fritter of 1cm, thickness 0.5cm, obtain block stock；
Above-mentioned block stock is placed at 50 DEG C and bakes 1.5h by II, the block stock after being baked；
III, mixes honey, sodium isoascorbate, Sodium Ascorbyl Phosphate, extract, water, wherein the honey, different
Sodium ascorbate, Sodium Ascorbyl Phosphate, extract, water mass ratio be 0.2:0.003:0.003:0.3:18, at 20 DEG C
Under 1h stirred with the revolving speed of 800r/min, obtain mixed liquor；Then by the mass volume ratio of 1:18 (g/mL) by the block after baking
Shape raw material is added in mixed liquor, and 10h is impregnated at 15 DEG C and is taken out, 15min is stood, and is placed in again at 45 DEG C after draining away the water dry
6h obtains the antitumor row liver poison Wild ganoderma product of anticancer.
The extract is mixed by licorice, chrysanthemum extract, dried orange peel extracts by the mass ratio of 1:1:1.
Embodiment 4
A kind of technology of preparing of the antitumor row liver poison Wild ganoderma product of anticancer, comprising the following steps:
It after I, cleans ganoderma applanatum, is placed at 110 DEG C and sterilizes 6s, then in 45 DEG C of dry 3h, then be cut into long 2cm, width
The fritter of 1cm, thickness 0.5cm, obtain block stock；
Above-mentioned block stock is placed at 50 DEG C and bakes 1.5h by II, the block stock after being baked；
III, mixes honey, Sodium Ascorbyl Phosphate, chitosan, extract, water, wherein the honey, ascorbic acid
Sodium phosphate, chitosan, extract, water mass ratio be 0.2:0.003:0.003:0.3:18, with 800r/min at 20 DEG C
Revolving speed stir 1h, obtain mixed liquor；Then the block stock after baking is added by the mass volume ratio of 1:18 (g/mL) mixed
It closes in liquid, 10h is impregnated at 15 DEG C and is taken out, 15min is stood, be placed at 45 DEG C dry 6h after draining away the water again, it is anti-to obtain anticancer
Tumour arranges liver poison Wild ganoderma product.
The extract is mixed by licorice, chrysanthemum extract, dried orange peel extracts by the mass ratio of 1:1:1.
Embodiment 5
A kind of technology of preparing of the antitumor row liver poison Wild ganoderma product of anticancer, comprising the following steps:
It after I, cleans ganoderma applanatum, is placed at 110 DEG C and sterilizes 6s, then in 45 DEG C of dry 3h, then be cut into long 2cm, width
The fritter of 1cm, thickness 0.5cm, obtain block stock；
Above-mentioned block stock is placed at 50 DEG C and bakes 1.5h by II, the block stock after being baked；
III, mixes honey, sodium isoascorbate, chitosan, extract, water, wherein the honey, arabo-ascorbic acid
Sodium, chitosan, extract, water mass ratio be 0.2:0.003:0.003:0.3:18, with the revolving speed of 800r/min at 20 DEG C
1h is stirred, mixed liquor is obtained；Then the block stock after baking is added in mixed liquor by the mass volume ratio of 1:18 (g/mL),
10h is impregnated at 15 DEG C to take out, stands 15min, is placed at 45 DEG C dry 6h after draining away the water again, is obtained the antitumor row liver of anticancer
Malicious Wild ganoderma product.
The extract is mixed by licorice, chrysanthemum extract, dried orange peel extracts by the mass ratio of 1:1:1.
Comparative example 1
A kind of technology of preparing of the antitumor row liver poison Wild ganoderma product of anticancer, comprising the following steps:
It after I, cleans ganoderma applanatum, is placed at 110 DEG C and sterilizes 6s, then in 45 DEG C of dry 3h, then be cut into long 2cm, width
The fritter of 1cm, thickness 0.5cm, obtain block stock；
Above-mentioned block stock is placed at 50 DEG C and bakes 1.5h by II, the block stock after being baked；
III, mixes honey, extract, water, wherein the mass ratio of the honey, extract, water is 0.2:0.3:18,
1h is stirred with the revolving speed of 800r/min at 20 DEG C, obtains mixed liquor；Then after being baked by the mass volume ratio of 1:18 (g/mL)
Block stock be added mixed liquor in, at 15 DEG C impregnate 10h take out, stand 15min, be placed in again after draining away the water at 45 DEG C do
Dry 6h obtains the antitumor row liver poison Wild ganoderma product of anticancer.
The extract is mixed by licorice, chrysanthemum extract, dried orange peel extracts by the mass ratio of 1:1:1.
Embodiment 6
Substantially the same manner as Example 2, difference is only in that: the extract is by licorice, dried orange peel extracts by 1:1
Mass ratio mix.
Embodiment 7
Substantially the same manner as Example 2, difference is only in that: the extract is by dried orange peel extracts, chrysanthemum extract by 1:1
Mass ratio mix.
Embodiment 8
Substantially the same manner as Example 2, difference is only in that: the extract is by licorice, chrysanthemum extract by 1:1
Mass ratio mix.
Comparative example 2
A kind of technology of preparing of the antitumor row liver poison Wild ganoderma product of anticancer, comprising the following steps:
It after I, cleans ganoderma applanatum, is placed at 110 DEG C and sterilizes 6s, then in 45 DEG C of dry 3h, then be cut into long 2cm, width
The fritter of 1cm, thickness 0.5cm, obtain block stock；
Above-mentioned block stock is placed at 50 DEG C and bakes 1.5h by II, the block stock after being baked；
III, mixes honey, sodium isoascorbate, Sodium Ascorbyl Phosphate, chitosan, water, wherein the honey, different
Sodium ascorbate, Sodium Ascorbyl Phosphate, chitosan, water mass ratio be 0.2:0.002:0.002:0.002:18,20
1h is stirred with the revolving speed of 800r/min at DEG C, obtains mixed liquor；It then will be after baking by the mass volume ratio of 1:18 (g/mL)
Block stock is added in mixed liquor, and 10h is impregnated at 15 DEG C and is taken out, 15min is stood, and is placed in again at 45 DEG C after draining away the water dry
6h obtains the antitumor row liver poison Wild ganoderma product of anticancer.
Embodiment 9
A kind of technology of preparing of the antitumor row liver poison Wild ganoderma product of anticancer, comprising the following steps:
It after I, cleans ganoderma applanatum, is placed at 110 DEG C and sterilizes 6s, then in 45 DEG C of dry 3h, then be cut into long 2cm, width
The fritter of 1cm, thickness 0.5cm, obtain block stock；
Above-mentioned block stock is placed at 50 DEG C and bakes 1.5h by II, the block stock after being baked；
III, surpasses in the block stock investment means of supercritical extraction equipment after baking under the carbon dioxide flow of 50L/h and faces
Boundary extracts 3h, wherein the extracting pressure of the extraction kettle is 30MPa, temperature is 45 DEG C；The pressure of knockout drum I is 11MPa, temperature
It is 35 DEG C；The pressure of knockout drum II is 5MPa, and temperature is 30 DEG C, and the block stock of supercritical extract processing is obtained after pressure release；
IV, mixes honey, sodium isoascorbate, Sodium Ascorbyl Phosphate, chitosan, extract, water, wherein described
Honey, sodium isoascorbate, Sodium Ascorbyl Phosphate, chitosan, extract, water mass ratio be 0.2:0.002:0.002:
0.002:0.3:18 stirs 1h at 20 DEG C with the revolving speed of 800r/min, obtains mixed liquor；Then the quality of 1:18 (g/mL) is pressed
The block stock that volume ratio handles supercritical extract is added in mixed liquor, and 10h is impregnated at 15 DEG C and is taken out, and stands 15min, drip
Dry 6h at 45 DEG C is placed in after solid carbon dioxide point again, obtains the antitumor row liver poison Wild ganoderma product of anticancer.
The extract is mixed by licorice, chrysanthemum extract, dried orange peel extracts by the mass ratio of 1:1:1.
Test case 1
Microbiological indicator test: wild to the antitumor row liver poison of anticancer of the present invention referring to the regulation of GB/4789.3-2010
The microbiological indicator of Garnoderma product is measured, and concrete outcome is shown in Table 1, and wherein MNP represents most probable number MPN in table 1.
Table 1: microbiological indicator test result table
By test result it is found that the antitumor row liver poison Wild ganoderma product of 2 anticancer of embodiment use by sodium isoascorbate,
The antioxidant fresh-keeping agent that Sodium Ascorbyl Phosphate, chitosan mix is handled, and microbiological indicator is better than embodiment 3-5
The antitumor row liver poison Wild ganoderma product of anticancer (is mixed by sodium isoascorbate, Sodium Ascorbyl Phosphate, any two kinds of chitosan
Antioxidant fresh-keeping agent made of conjunction) and not using the comparative example 1 of antioxidant fresh-keeping agent processing.
Test case 2
The inhibiting rate of cancer cell multiplication: with reference to journal of writings " proliferation suppression of the Ah pa for Buddhist nun to hepatocellular carcinoma H22 of Chen Tao
Production use " in detection method, using the antitumor row liver poison Wild ganoderma product of MTT colorimetric determination anticancer of the present invention to people liver
The inhibited proliferation of cancer cell HepG2.The wherein cultural method of cancer cell are as follows: human hepatoma cell strain Hep G2 (is purchased from
The Chinese Academy of Sciences cell institute (Shanghai) cell bank) with containing 10% fetal calf serum and 100 μ g/ml Pen .- Streps
RPMI1640 culture medium (is purchased from U.S. HyClone company), is placed in 37 DEG C, 5%CO2, it is constant-temperature enclosed under the conditions of saturated humidity
It cultivates and passes in incubator (being purchased from U.S. NBS company), every 3-4d changes liquid passage.Experimental method: first by the antitumor row of anticancer
Liver poison Wild ganoderma product is crushed to 200 mesh, obtains the antitumor row liver poison Wild ganoderma product powder of anticancer；Then to being vaccinated with
The antitumor row liver poison Wild ganoderma product powder of anticancer is added in 96 porocyte culture plates of human liver cancer cell HepG2, wherein resisting
The intervention concentration of the antitumor row liver poison Wild ganoderma product of cancer measures absorbance after being 300 μ g/mL, 72h and calculates cancer cell increasing
The inhibiting rate grown, specific calculated result are shown in Table 2.
Table 2: the inhibiting rate of cancer cell multiplication
| Group | Inhibiting rate/% of cancer cell multiplication |
| Embodiment 1 | 51.05 |
| Embodiment 2 | 75.29 |
| Embodiment 3 | 70.77 |
| Embodiment 4 | 69.62 |
| Embodiment 5 | 70.18 |
| Embodiment 6 | 64.80 |
| Embodiment 7 | 63.95 |
| Embodiment 8 | 65.41 |
| Embodiment 9 | 88.15 |
| Comparative example 2 | 56.34 |
By test result it is found that the use of embodiment 2 is mixed by licorice, chrysanthemum extract, dried orange peel extracts
Extract handled, the inhibiting rate of cancer cell multiplication is higher than embodiment 6-8, and (extract is mentioned by licorice, chrysanthemum
Any two kinds of object, dried orange peel extracts are taken to mix) and using the comparative example 2 of extract-treated；And embodiment 9 is further adopted
Raw material is handled with the method for supercritical extract, liposoluble constituent in raw material is preferentially extracted from raw material, many skies are formed
Gap, structure is more loose, expands dialysis membrane diameter, increases the surface area contacted with water, improves in raw material such as ganoderma lucidum polysaccharide
The dissolution rate of equal water soluble ingredients further improves the inhibiting rate of cancer cell multiplication for embodiment 2.
The above described is only a preferred embodiment of the present invention, limitation in any form not is done to the present invention, therefore
The equivalent or simple change that all principles described according to the invention patent design are done, is included in the scope of protection of the patent of the present invention
It is interior；Those skilled in the art can make various modifications or additions to the described embodiments
Or be substituted in a similar manner, without departing from structure of the invention or beyond the scope defined by this claim,
It is within the scope of protection of the invention.
Claims (8)
1. a kind of technology of preparing of the antitumor row liver poison Wild ganoderma product of anticancer, which comprises the following steps:
I, ganoderma applanatum is cleaned, after sterilization processing, is placed at 40-50 DEG C and is dried, stripping and slicing obtains block stock；
II, at 35-60 DEG C to above-mentioned block stock baked to get.
2. the technology of preparing of the antitumor row liver poison Wild ganoderma product of anticancer as described in claim 1, which is characterized in that including with
Lower step:
I, ganoderma applanatum is cleaned, after sterilization processing, is placed at 40-50 DEG C and is dried, stripping and slicing obtains block stock；
II, bakes above-mentioned block stock at 35-60 DEG C, the block stock after being baked；
III, mixes honey, antioxidant fresh-keeping agent, extract, water, wherein the matter of the honey, antioxidant fresh-keeping agent, extract, water
Amount is than being (0.1-0.5): (0.005-0.015): (0.1-0.5): (10-20) obtains mixed liquor after mixing；Then 1:(10- is pressed
20) block stock after baking is added in mixed liquor the mass volume ratio of (g/mL), is impregnated at 10-20 DEG C, takes out
After drain away the water, it is dry, obtain the antitumor row liver poison Wild ganoderma product of anticancer.
3. the technology of preparing of the antitumor row liver poison Wild ganoderma product of anticancer as described in claim 1, which is characterized in that including with
Lower step:
I, ganoderma applanatum is cleaned, after sterilization processing, is placed at 40-50 DEG C and is dried, stripping and slicing obtains block stock；
II, bakes above-mentioned block stock at 35-60 DEG C, the block stock after being baked；
III, is carried out in the block stock investment means of supercritical extraction equipment after baking under the carbon dioxide flow of 50-100L/h
Supercritical extract processing, obtains the block stock of supercritical extract processing；
IV, mixes honey, antioxidant fresh-keeping agent, extract, water, wherein the matter of the honey, antioxidant fresh-keeping agent, extract, water
Amount is than being (0.1-0.5): (0.005-0.015): (0.1-0.5): (10-20) obtains mixed liquor after mixing；Then 1:(10- is pressed
20) block stock that the mass volume ratio of (g/mL) handles supercritical extract is added in mixed liquor, is soaked at 10-20 DEG C
Bubble, drains away the water after taking-up, dry, obtains the antitumor row liver poison Wild ganoderma product of anticancer.
4. the technology of preparing of the antitumor row liver poison Wild ganoderma product of the anticancer as described in claim any one of 1-3, feature exist
In the sterilization temperature of the sterilization processing is 100-120 DEG C, sterilizing time 5-10s.
5. the technology of preparing of the antitumor row liver poison Wild ganoderma product of the anticancer as described in Claims 2 or 3, which is characterized in that institute
Stating antioxidant fresh-keeping agent includes one of sodium isoascorbate, Sodium Ascorbyl Phosphate, chitosan or a variety of.
6. the technology of preparing of the antitumor row liver poison Wild ganoderma product of the anticancer as described in Claims 2 or 3, which is characterized in that institute
Stating extract includes one of licorice, chrysanthemum extract, dried orange peel extracts or a variety of.
7. the technology of preparing of the antitumor row liver poison Wild ganoderma product of anticancer as claimed in claim 3, which is characterized in that described super
Critical extraction processing is circulating using two points of an extraction, and wherein the extracting pressure of extraction kettle is 30-50MPa, and temperature is 40-50 DEG C；
The pressure 11-15MPa of knockout drum I, temperature are 30-35 DEG C；The pressure of knockout drum II is 5-6MPa, and temperature is 30-35 DEG C.
8. a kind of antitumor row liver poison Wild ganoderma product of anticancer, which is characterized in that described anti-using any one of claim 1-7
The technology of preparing of the antitumor row liver poison Wild ganoderma product of cancer is prepared.
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Application publication date: 20181211 |