CN108939156A - 3D printing Ti-PDA-BMP-2 bone defect healing tissue engineering bracket and preparation method thereof - Google Patents
3D printing Ti-PDA-BMP-2 bone defect healing tissue engineering bracket and preparation method thereof Download PDFInfo
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- CN108939156A CN108939156A CN201810616827.3A CN201810616827A CN108939156A CN 108939156 A CN108939156 A CN 108939156A CN 201810616827 A CN201810616827 A CN 201810616827A CN 108939156 A CN108939156 A CN 108939156A
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L27/00—Materials for grafts or prostheses or for coating grafts or prostheses
- A61L27/28—Materials for coating prostheses
- A61L27/34—Macromolecular materials
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L27/00—Materials for grafts or prostheses or for coating grafts or prostheses
- A61L27/02—Inorganic materials
- A61L27/04—Metals or alloys
- A61L27/06—Titanium or titanium alloys
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L27/00—Materials for grafts or prostheses or for coating grafts or prostheses
- A61L27/50—Materials characterised by their function or physical properties, e.g. injectable or lubricating compositions, shape-memory materials, surface modified materials
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L27/00—Materials for grafts or prostheses or for coating grafts or prostheses
- A61L27/50—Materials characterised by their function or physical properties, e.g. injectable or lubricating compositions, shape-memory materials, surface modified materials
- A61L27/54—Biologically active materials, e.g. therapeutic substances
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L27/00—Materials for grafts or prostheses or for coating grafts or prostheses
- A61L27/50—Materials characterised by their function or physical properties, e.g. injectable or lubricating compositions, shape-memory materials, surface modified materials
- A61L27/56—Porous materials, e.g. foams or sponges
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B33—ADDITIVE MANUFACTURING TECHNOLOGY
- B33Y—ADDITIVE MANUFACTURING, i.e. MANUFACTURING OF THREE-DIMENSIONAL [3-D] OBJECTS BY ADDITIVE DEPOSITION, ADDITIVE AGGLOMERATION OR ADDITIVE LAYERING, e.g. BY 3-D PRINTING, STEREOLITHOGRAPHY OR SELECTIVE LASER SINTERING
- B33Y10/00—Processes of additive manufacturing
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B33—ADDITIVE MANUFACTURING TECHNOLOGY
- B33Y—ADDITIVE MANUFACTURING, i.e. MANUFACTURING OF THREE-DIMENSIONAL [3-D] OBJECTS BY ADDITIVE DEPOSITION, ADDITIVE AGGLOMERATION OR ADDITIVE LAYERING, e.g. BY 3-D PRINTING, STEREOLITHOGRAPHY OR SELECTIVE LASER SINTERING
- B33Y70/00—Materials specially adapted for additive manufacturing
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B33—ADDITIVE MANUFACTURING TECHNOLOGY
- B33Y—ADDITIVE MANUFACTURING, i.e. MANUFACTURING OF THREE-DIMENSIONAL [3-D] OBJECTS BY ADDITIVE DEPOSITION, ADDITIVE AGGLOMERATION OR ADDITIVE LAYERING, e.g. BY 3-D PRINTING, STEREOLITHOGRAPHY OR SELECTIVE LASER SINTERING
- B33Y80/00—Products made by additive manufacturing
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L2300/00—Biologically active materials used in bandages, wound dressings, absorbent pads or medical devices
- A61L2300/40—Biologically active materials used in bandages, wound dressings, absorbent pads or medical devices characterised by a specific therapeutic activity or mode of action
- A61L2300/412—Tissue-regenerating or healing or proliferative agents
- A61L2300/414—Growth factors
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L2300/00—Biologically active materials used in bandages, wound dressings, absorbent pads or medical devices
- A61L2300/60—Biologically active materials used in bandages, wound dressings, absorbent pads or medical devices characterised by a special physical form
- A61L2300/606—Coatings
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L2420/00—Materials or methods for coatings medical devices
- A61L2420/02—Methods for coating medical devices
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L2430/00—Materials or treatment for tissue regeneration
- A61L2430/02—Materials or treatment for tissue regeneration for reconstruction of bones; weight-bearing implants
Abstract
The invention discloses 3D printing Ti-PDA-BMP-2 bone defect healing tissue engineering brackets and preparation method thereof.3D printing Ti-PDA-BMP-2 bone defect healing tissue engineering bracket of the present invention is prepared by 3D printing technique with controllable shape, porosity micro-structure and the controllable bracket of physicochemical property by Ti6Al4V powder, have the biotic factor BMP-2 of Bone Defect Repari ability by the poly-dopamine adherency of fiber surface, can be used for the repairing and treating of bone defect.The present invention has simple and reliable for structure, and shape is controllable with micro-structure, and the modified simple and effective in surface, mechanical property is reliable, bioactivity and highly-safe, and implantation facilitates, the advantage that wound is small, at low cost.
Description
Technical field
The invention belongs to bone tissue reparation and field is rebuild, is related to 3D printing Ti-PDA-BMP-2 bone defect healing group weaver
Engineering support and preparation method thereof.
Background technique
The repair and reconstruction of bone defect are one of Orthopedic Clinical problems faceds.It is well known that autologous bone is considered as bone
The goldstandard of transplanting, but its limited source and downright bad, the postoperative chronic ache, allergic reaction and the sense that may cause donor site
The complication such as dye.And homogeneous allogenic bone and bone- xenograft are answered extensively due to from a wealth of sources and not needing additional surgical procedure
With, but shifting is especially reduction of there is also the complication such as slow integration and remodeling, immunological rejection and transmission simultaneously
The osteoinductive and osteoconductive of plant.
Timbering material has bioactivity calcium phosphate ceramics, calcium phosphate bone cement (CPC), hydroxyapatite (HA), coral at present
Coral, gypsum；Polylactic acid (PLA), polyglycolic acid (PGA)；Collagen, decalcified bone matrix (DBM), fibrin clot (FC) etc..And
Ideal extracellular matrix material should have following condition: (1) good biocompatibility, after implanting, no matter its
Itself or its catabolite all cope with body and have no toxic side effect, and will not generate inflammatory reaction, will not cause the graft rejection of host
Reaction.(2) degradation rate of material must match with the generating rate of new bone, can be complete after material completes bracket effect
It absorbs.(3) it is at least up to 90% or more three-dimensional structure and certain toughness with porosity, provides height for histocyte
The contact surface area of ratio and growing space.(4) material can not only maintain cellular morphology and phenotype, and cell can be promoted viscous
Attached, proliferation, inducing tissue regeneration.Although the porous ceramic film material studied at present, high molecular material and its their composite material
Etc. can be used as potential bone collection timbering material, but there are various drawbacks in them, be not able to satisfy load receiving part especially
The requirement of position bone tissue reparation.In recent years, the potential application of the metallic support of three-dimensional porous structure had attracted more and more
The attention of researcher, the metallic support of three-dimensional porous structure are considered a kind of very promising bone grafting material and are widely applied
In orthopaedics and dental field.Compared with ceramics, polymer material, its higher mechanical strength of because of metal and fracture toughness and be suitble to
The application of weight bearing area.In numerous metal materials, titanium and its alloy are because of its good biocompatibility, biological safety, good
The advantages that good mechanical property and corrosion resistance and be widely used.
But therefore titanium has carried out numerous bases as timbering material, the same problem lower with bioactivity at present
In the compound research of the modification of material itself and seed cell and ostosis and the relevant growth factor of absorption.Material itself changes
Property rely primarily on the processing of chemical factors, including soda acid processing, electromagnetism and redox mode introduce other elements to expect
The bioactivity of lifting bracket.These methods are i.e. complex in bracket preparation process, and homogeneity is difficult to be controlled.And
Seed cell is the critical issue of tissue-engineered bone.There are ideal seed cell materials to be easy, small to body injury；In vitro
Amplification is easy, and expression is stablized；Implant no immunological rejection, has the stronger ability of skeletonization, and without oncogenicity.It studies at present more
Have osteoblast, marrow stromal cell, mescenchymal stem cell, fat stem cell and embryonic stem cell etc..But for metal
For bracket, lower cell carrying rate is still the bottleneck for hindering it further to develop.As for growth factor, understanding is had now been found that
Ostosis relevant have bone morphogenetic protein (bone morphogenetic proteins, BMP), Peritoneal fibrosis
β (transforming growth factor- β, TGF-β), insulin-like growth factor (insulin-like growth
Factor, IGF), basic fibroblast growth factor (fibroblast growth factorFGF), it is platelet-derived because
Son (platelet-derived growth factor, PDGF), vascular endothelial growth factor
(vascu2larendothelial growth factor, VEGF) etc..But there are immunological rejections, cause of disease for bioactie agent
Body propagation, allergic reaction, potential cause tumour, teratogenesis shape possibility, biological safety cannot be guaranteed, and clinical grade
The biotic factors such as rhBMP-2, TGF-β generally require import, and expensive, and every milligram of purchasing price just reaches nearly thousand dollars.
In conclusion developing, one kind is simple and reliable for structure, and mechanical strength is suitable, and operation implantation is convenient, to human body wound
It is small, low cost, can induce the tissue-engineered bone of Bone Ingrowth with the repairing and treating of row bone defect to the Development of Health Service in China,
To construction, there is important realistic meaning in innovative country.
Summary of the invention
The purpose of the present invention is being directed to the above-mentioned deficiency of the prior art, a kind of 3D printing Ti-PDA-BMP-2 bone defect is provided
Repair tissue engineering rack.
It is a further object of the present invention to provide the systems of 3D printing Ti-PDA-BMP-2 bone defect healing tissue engineering bracket
Preparation Method.
The purpose of the present invention can be achieved through the following technical solutions:
A kind of 3D printing Ti-PDA-BMP-2 bone defect healing tissue engineering bracket, by Ti6Al4V through 3D printing technique system
The standby 3D printing Ti bracket with porous structure, PDA coating and PDA the coating surface absorption of the fiber surface of Ti bracket
BMP-2 composition.
The 3D printing Ti-PDA-BMP-2 bone defect healing tissue engineering bracket, preferably will by laser sintering technology
The powder sintered shaping fiber beam out of Ti6Al4V is configured standby 3D printing Ti by the different levels of fibre bundle and the joggle truss of angle
Bracket；Dopamine is then made to form PDA coating in the fiber surface auto polymerization of 3D printing Ti bracket under certain condition, thus
Prepare 3D printing Ti-PDA bracket；Finally, gained 3D printing Ti-PDA bracket is applied by the method for absorption biotic factor in PDA
Layer surface loads BMP-2, and 3D printing Ti-PDA-BMP-2 bone defect healing tissue engineering bracket is prepared.
Wherein, layer-by-layer accumulation of the fiber of 3D printing by different levels and angle and porous knot made of splicing framework
Structure, hole are that each layer fiber intersects in the range of 0-180 ° and accumulate and formed, ultimately form 0-90 ° of orthogonal row structure and
Regularity hole as 0-60-120 ° of triangular structure, or gradient distribution, stratification phase knot are formed as needed according to practical
Structure.
The preparation method of 3D printing Ti-PDA-BMP-2 bone defect healing tissue engineering bracket of the present invention, by swashing
Light sintering technology by Ti6Al4V it is powder sintered go out shaping fiber beam, pass through the different levels of fibre bundle and the splicing framework of angle
Prepare 3D printing Ti bracket；Then form dopamine in the fiber surface auto polymerization of 3D printing Ti bracket
PDA coating, to prepare 3D printing Ti-PDA bracket；Finally, gained 3D printing Ti-PDA bracket is passed through absorption biotic factor
Method PDA coating surface load BMP-2,3D printing Ti-PDA-BMP-2 bone defect healing organizational project branch is prepared
Frame.
Preparation method of the present invention, preferably comprises following steps:
(1) 3D printing Ti bracket is prepared
(2) prepare 3D printing Ti-PDA bracket: preparation DA-Tris-HCL solution, wherein the concentration of dopamine is 2g/L-
3D printing Ti bracket is added in above-mentioned solution, is protected from light, stirs under blow-by state, to DA in Ti fiber surface autohemagglutination by 8g/L
Conjunction takes out bracket after forming PDA, obtains 3D printing Ti-PDA bracket；
(3) 3D printing Ti-PDA-BMP-2 bone defect healing tissue engineering bracket: the 3D printing Ti- that will have been prepared is prepared
PDA bracket is soaked in the BMP-2 aqueous solution containing 25 or 50 or 100 microgram μ g/mL, by 3D printing Ti-PDA rack surface
Suction-operated of the phenolic hydroxyl group, nitrogen-containing group contained in PDA to BMP-2 in solution, is fixed in matrix surface, impregnate 8,
16, it takes out bracket afterwards for 24 hours, realizes the Ti-PDA-BMP-2 bone defect healing tissue engineering bracket of various concentration.
Wherein, Ti6Al4V powder is further preferably passed through laser by the specific method of step (1) preparation 3D printing Ti bracket
Sintering forms shaping fiber beam, obtains 3D printing product by the different levels of fibre bundle and the splicing framework of angle；Its shape
Bone defect form according to different areas to be repaired carries out 3D printing individual character manufacturing, or is fabricated to outside fixed by SLM technology
The timbering material of shape.
The hole of the 3D printing Ti bracket is regularity hole as 0-90 ° of orthogonal row structure, or according to reality
Need to form special gradient distribution, stratification intersection.
The utility model has the advantages that
(1) micro-structure controllable characteristics: the present invention is to be tired out with laser sintered fiber by different levels and the layer-by-layer of angle
Product with multi-pore structure made of splicing framework, each layer fiber in hole can intersect in the range of 0-180 ° and accumulate and
It is formed, the distance between each fiber can also influence the size of hole, may finally thus form a variety of intersection constructions
Regular hole can also form special gradient distribution, stratification intersection according to actual needs；Can be thus achieved shape,
The precise controlling of internal pore structure；
(2) simplicity and biotic factor slow release characteristic of surface modification: 3D printing micro-structure of the invention itself includes bracket
Hole size, hole shape, hole orientation, porosity and connectivity and bracket rough surface characteristic etc. can regulate and control the cell of bracket
Adhesion property.And be surface modified using poly-dopamine, be not only simple and obviously improve metallic support hydrophily and
Biocompatibility.And the suction-operated of poly-dopamine can promote the carrying efficiency of growth factor, and can be realized it is biological because
Son sustained release, meanwhile, by change manufacturing process in the PDA reaction time, the concentration of biotic factor, biotic factor absorption when
Between, it can further regulate and control the load capacity of rack surface biotic factor, and then regulate and control the release characteristics of biotic factor.
(3) the controllable characteristic of mechanical property and good Bone Ingrowth characteristic and biological safety: porous micro- knot of the invention
Structure, Ti alloy material have preferable elasticity modulus and between human Cortex's bone and the elasticity modulus of cancellous bone, can be compared with
Precisely match the mechanical environment in area to be repaired, according to the Wolf law of bone uptake, be beneficial to new bone grow into and moulding；And
Ti itself there is good biocompatibility can be provided with conducive to stem cell adherency, amplification, Osteoblast Differentiation and bone matrix accumulation
Local microenvironment.Meanwhile the modification of poly-dopamine can further improve the hydrophily and biocompatibility of rack surface.It carries
Biotic factor can slow release, realize defective region efficient repair process for a long time, meanwhile, porous structure is conducive to seek
The exchange formed point, and then can promote growing into for new bone.The Ti metal material that the present invention uses early is widely used in orthopaedics
In the clinical use of glove, and poly-dopamine and BMP-2 are proved its safety, the entire biological safety for repairing system compared with
Other modification schemes are considerably higher, and osteogenic activity is obviously improved.
Novel 3D printing Ti-PDA-BMP-2 bone defect healing tissue engineering bracket provided by the invention, it is existing having
The performance that 3D printing technique, timbering material itself have also been given full play to while bone renovating material advantage, so that the present invention has
Simple and reliable for structure, shape is controllable with micro-structure, and the modified simple and effective in surface, mechanical property is reliable, bioactivity and safety
Height, implantation is convenient, the advantage that wound is small, at low cost, and the reparation that can be used for bone defect after bone wound, bone tumour, infection of bone is controlled
It treats.
Detailed description of the invention
Fig. 1 is the skill that Bone Defect Repari is carried out using 3D printing Ti-PDA-BMP-2 bone defect healing tissue engineering bracket of the present invention
Art route map.
For bone defect patient, removing metallic foreign body etc. may influence to sweep outside the disturbing factor of CT scan image by CT
Acquisition anatomical data is retouched, and combines the coincident with severity degree of condition of bone defect, design has personalized shape and the 3D of physicochemical property is beaten
Print bracket is simultaneously implanted into bone defect region.For the patient of precise anatomical data can not be obtained, preprepared 3D printing is used
Ti-PDA-BMP-2 bone defect healing tissue engineering bracket determines the quantity of implantation bracket according to actual defect situation.Bracket is planted
Ru Hou freshman bone tissue then constantly grows into, the final target for realizing repairing bone defect reparation.
Fig. 2 is fibre lay-up arrangement of the invention, the schematic diagram for establishing three-dimensional porous structure.
Fig. 2A B is the two-dimensional representation that scaffold fibers are arranged under the overlay according to different levels, it can be seen that the pore morphology of bracket
It is determined by the stacking of scaffold fibers, including hole size, hole shape, hole orientation, porosity and connectivity etc..Fig. 2 C is to provide to adopt
The structural schematic diagram with the 3D printing Ti bracket of square is formed with 0-90 ° of stacked arrangement.
Fig. 3 provides the structural representation for being formed using 0-60-120 ° of stacked arrangement and having the 3D printing Ti bracket of triangle
Figure.It in actual operation, can also be according to the actual requirement of difference area's mechanical environment to be repaired, bone defect degree etc., by right
The regulation of scaffold fibers stacking, and then the bracket for preparing personalized designs, having specific pore micro-structure.
Fig. 4 is the technology path schematic diagram that the present invention prepares bracket using General design and 3D printing technique.
1 is Ti6Al4V powder；The 2 3D printing schematic diagrames prepared for bracket, pass through laser sintered preparation 3D printing for powder
Ti bracket；3 be to carry out PDA coating to the Ti bracket of acquisition to prepare 3D printing Ti-PDA bracket；4 be by 3D printing Ti-PDA branch
Frame is mixed with the solution containing BMP-2, prepares 3D printing Ti-PDA-BMP-2 bone defect healing tissue engineering bracket；5 be to obtain
The general 3D printing Ti-PDA-BMP-2 bone defect healing tissue engineering bracket taken.
Fig. 5 is to play the schematic diagram for promoting bone tissue to grow into biological action after the present invention is implanted into bone defect area.
1 is normal bone tissues；2 be stenter to implant bone defect position；Single branch after 3 display 3D printing Ti bracket partial enlargements
The schematic diagram of biotic factor on frame fiber；4 be the release of the various factors of Ti scaffold fibers；5 release for rack surface and interior factor
It puts, the recruitment of osteoblast and bone tissue are grown into；6 for bone tissue grow into and with neighboring area integration, bone defect area
It gradually repairs in domain.
Fig. 6 be A and B be respectively 0-90 ° of 3D printing Ti bracket pictorial diagram and its poly-dopamine modification after substantially according to.C,D
After the pictorial diagram and DOPA of respectively 0-60-120 ° structure stand are amine-modified substantially according to.
Fig. 7 is the result figure of present invention Bone Defect Repari experiment in vivo.
A is bone defect region；B is that stenter to implant bone defect region is shown that 3D printing bracket is completely embedded into bone defect area
Domain；C is grown into for bone tissue after stenter to implant January；D, E be respectively stenter to implant 2 months, the photo in March, show area of new bone bone
Tissue is grown into.
Specific embodiment
Embodiment 1
1. preparing 3D printing Ti bracket
Ti6Al4V powder is passed through into laser sintered formation shaping fiber beam, passes through 0-90 ° or 0-60-120 ° layer of fibre bundle
Stack arrangement splices the 3D printing Ti bracket that framework obtains, as shown in Figure 6.
2. preparing 3D printing Ti-PDA bracket
After the completion of supporting structure is constructed by 3D printing, it is surface modified using PDA, specific steps include:
1) compound concentration is hydrochloric acid (HCL) solution of 0.5mol/L；
2) it weighs 0.61gTris and is added in 500ml water and dissolve, added dropwise after stirring with the 0.5mol/L HCL solution prepared
Enter to be adjusted to pH=8.5, is configured to Tris-HCL solution for standby；
3) dopamine (Dopamine, DA) is dissolved in above-mentioned 150ml Tris-HCL solution, and stirring and dissolving is formulated as 2%
DA-Tris-HCL solution；
4) 3D printing Ti bracket is added in above-mentioned solution, is protected from light, is stirred under blow-by state, 800-1000r/ minutes,
Reaction time takes out bracket after Ti fiber surface auto polymerization forms PDA for 24 hours, after DA, obtains 3D printing Ti-PDA bracket.
3. preparing 3D printing Ti-PDA-BMP-2 bone defect healing tissue engineering bracket
The 3D printing Ti-PDA bracket prepared is soaked in the BMP-2 aqueous solution containing 25 or 50 or 100 μ g/mL
In, the suction-operated by the phenolic hydroxyl group, nitrogen-containing group contained in 3D printing Ti-PDA rack surface PDA to BMP-2 in solution,
Be fixed in matrix surface, impregnate 8,16, take out bracket afterwards for 24 hours, realize that the Ti-PDA-BMP-2 bone defect of various concentration is repaired
Overlying tissue engineering rack, as shown in Figure 6.
The bone of the 3D printing Ti-PDA-BMP-2 bone defect healing tissue engineering bracket implantation in rabbit of preparation is lacked by performing the operation
Region is damaged, as a result as shown in Figure 7.
Claims (7)
1. a kind of 3D printing Ti-PDA-BMP-2 bone defect healing tissue engineering bracket, it is characterised in that beaten by Ti6Al4V through 3D
The 3D printing Ti bracket with porous structure of print technology preparation, the PDA coating and PDA coating table of the fiber surface of Ti bracket
The BMP-2 composition of face absorption.
2. 3D printing Ti-PDA-BMP-2 bone defect healing tissue engineering bracket according to claim 1, it is characterised in that
By laser sintering technology by Ti6Al4V it is powder sintered go out shaping fiber beam, pass through the spelling of the different levels and angle of fibre bundle
Connect framework preparation 3D printing Ti bracket；Then make dopamine in the fiber surface autohemagglutination of 3D printing Ti bracket under certain condition
Conjunction forms PDA coating, to prepare 3D printing Ti-PDA bracket；Finally, gained 3D printing Ti-PDA bracket is passed through absorption life
The method of the object factor loads BMP-2 in PDA coating surface, and 3D printing Ti-PDA-BMP-2 bone defect healing group weaver is prepared
Engineering support.
3. 3D printing Ti-PDA-BMP-2 bone defect healing tissue engineering bracket according to claim 1, it is characterised in that
Layer-by-layer accumulation of the fiber of 3D printing by different levels and angle and multi-pore structure made of splicing framework, hole is each layer
Fiber intersects in the range of 0-180 ° and accumulates and formed, and ultimately forms 0-90 ° of orthogonal row structure or 0-60-120 ° of triangle
Regularity hole as structure, or gradient distribution, stratification intersection are formed as needed according to practical.
4. the preparation method of 3D printing Ti-PDA-BMP-2 bone defect healing tissue engineering bracket described in claim 1, special
Sign be by laser sintering technology by Ti6Al4V it is powder sintered go out shaping fiber beam, the different levels by fibre bundle and angle
The joggle truss of degree is configured standby 3D printing Ti bracket；Then make dopamine in the fiber table of 3D printing Ti bracket under certain condition
Face auto polymerization forms PDA coating, to prepare 3D printing Ti-PDA bracket；Finally, gained 3D printing Ti-PDA bracket is passed through
The method for adsorbing biotic factor loads BMP-2 in PDA coating surface, and 3D printing Ti-PDA-BMP-2 bone defect healing is prepared
Tissue engineering bracket.
5. the preparation method according to claim 4, it is characterised in that comprise the steps of:
(1) 3D printing Ti bracket is prepared
(2) prepare 3D printing Ti-PDA bracket: preparation DA-Tris-HCL solution, wherein the concentration of dopamine is 2g/L-8g/L,
3D printing Ti bracket is added in above-mentioned solution, is protected from light, stirred under blow-by state, formed to DA in Ti fiber surface auto polymerization
Bracket is taken out after PDA, obtains 3D printing Ti-PDA bracket；
(3) the 3D printing Ti-PDA bracket prepared is soaked in the BMP-2 aqueous solution containing 25 or 50 or 100 μ g/mL,
Suction-operated by the phenolic hydroxyl group, nitrogen-containing group contained in 3D printing Ti-PDA rack surface PDA to BMP-2 in solution, will
It is fixed on matrix surface, impregnate 8,16, take out bracket afterwards for 24 hours, realize the Ti-PDA-BMP-2 bone defect healing of various concentration
Tissue engineering bracket.
6. the preparation method according to claim 4, it is characterised in that the specific method of step (1) preparation 3D printing Ti bracket
It is by Ti6Al4V powder by laser sintered formation shaping fiber beam, by the different levels of fibre bundle and the joggle truss of angle
Structure obtains 3D printing product；Its shape carries out 3D printing individual character manufacturing according to the bone defect form in different areas to be repaired, or
The timbering material of fixed profile is fabricated to by SLM technology.
7. preparation method according to claim 6, it is characterised in that the hole of the 3D printing Ti bracket be 0-90 ° just
Regularity hole as Bank of Communications's structure or 0-60-120 ° of triangular structure, or according to actual needs formed special gradient distribution,
Stratification intersection.
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Cited By (5)
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| CN109568674A (en) * | 2018-12-28 | 2019-04-05 | 上海纳米技术及应用国家工程研究中心有限公司 | Carry preparation method of the bionical bone repair porous scaffold of Types of Medicine and products thereof and application |
| CN110403736A (en) * | 2019-08-09 | 2019-11-05 | 常州市第二人民医院 | A kind of super porous titanium alloy of 3D printing facilitates bone surface method of modifying |
| CN111643725A (en) * | 2020-06-01 | 2020-09-11 | 四川大学 | Artificial bone material for repairing bone defect and preparation method of artificial bone particles |
| CN113476658A (en) * | 2021-07-16 | 2021-10-08 | 北京理工大学重庆创新中心 | Bessel beam-based bone joint implant surface treatment method |
| CN114515356A (en) * | 2022-03-11 | 2022-05-20 | 福州大学 | Preparation method of bionic bone tissue engineering scaffold capable of resisting inflammation and promoting bone defect repair |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN114099778A (en) * | 2021-09-30 | 2022-03-01 | 上海市浦东新区人民医院 | Composite scaffold for bone tissue engineering |
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| CN111643725A (en) * | 2020-06-01 | 2020-09-11 | 四川大学 | Artificial bone material for repairing bone defect and preparation method of artificial bone particles |
| CN113476658A (en) * | 2021-07-16 | 2021-10-08 | 北京理工大学重庆创新中心 | Bessel beam-based bone joint implant surface treatment method |
| CN114515356A (en) * | 2022-03-11 | 2022-05-20 | 福州大学 | Preparation method of bionic bone tissue engineering scaffold capable of resisting inflammation and promoting bone defect repair |
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| CN108939156B (en) | 2020-12-08 |
| CN112107726A (en) | 2020-12-22 |
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