CN102058904A - Biological functionalized nano titanium material and preparation method thereof - Google Patents
Biological functionalized nano titanium material and preparation method thereof Download PDFInfo
- Publication number
- CN102058904A CN102058904A CN2010106185709A CN201010618570A CN102058904A CN 102058904 A CN102058904 A CN 102058904A CN 2010106185709 A CN2010106185709 A CN 2010106185709A CN 201010618570 A CN201010618570 A CN 201010618570A CN 102058904 A CN102058904 A CN 102058904A
- Authority
- CN
- China
- Prior art keywords
- titanium material
- nano
- tio
- tube array
- dopamine
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Images
Abstract
The invention belongs to the field of biomedical materials, and relates to a biological functionalized nano titanium material. The surface of the titanium material is provided with a TiO2 nano tube array with tube diameter of 30-100nm, and the surface of the TiO2 nano tube array is also fixed with a bone morphogenetic protein 2(BMP2). A preparation method of the biological functionalized nano titanium material comprises the steps of: preparing the TiO2 nano tube array with the tube diameter of 30-100nm on the surface of the titanium material by adopting an anode oxidizing method, and fixing the BMP2 on the surface of the TiO2 nano tube array through polydopamine to obtain the biological functionalized nano titanium material. The biological functionalized nano titanium material has better biocompatibility; on one hand, the TiO2 nano tube array with proper tube diameter can provide a proper topological structure for the cell growth, on the other hand, the BMP2 is used as a growth factor capable of promoting the cell differentiation, and the proliferation and differentiation capacity of the cells on the surface of the titanium material can be improved through the synergistic effect of the topological structure and the chemical components, thus the osseointegration of the titanium material is improved.
Description
Technical field
The invention belongs to field of biomedical materials, relate to a kind of biological functional nanometer titanium material, also relate to the preparation method of this titanium material.
Background technology
Early 1940s, people such as Bothe implant titanium and find no any untoward reaction between itself and the bone in the femur of mouse, thereby titanium is incorporated into field of biomedical materials.Since the sixties in 20th century, titanium implant was widely used in the stomatology field, titanium had obtained continuous development as planting material, and along with deepening continuously of studying, the titanium material is generally believed it is up-and-coming medical science embedded material.
Because titanium or titanium alloy has biologically inert, the bone conformability is poor, can not combine with the human body surrounding tissue well, has shortcomings such as healing time length, primary stability difference, cause titanium implant to be implanted to power lower.For improving the biological activity of titanium or titanium alloy, further improve its bone conformability, the Surface Engineering research of titanium or titanium alloy has become a big focus of field of biomedical materials.
There are some researches show that material is relevant with the appearance structure of material surface in the intravital bone conformability of machine.Because the interface that cell is contacted in body is nanostructured, as the basement membrane of various tissues recessed, prominent, the hole and fibrous by 50 ~ 200nm size, therefore the surface topography at the bionical structure nanoscale of material surface may cell growth have positive impact.For example, make up nanostructured, be expected to improve the biological activity of titanium material, it can be responded under the physiological signal stimulation of organism microenvironment, reach the purpose of self-regeneration with cellular response on titanium material surface.Except the appearance structure of material surface can influence the cell behavior, the chemical constituent of material surface also can cell growth produce certain influence.Finishing is the important method that changes the material surface chemical constituent.For example, by chemistry or physical method, somatomedin, hormone or other chemical substance can be fixed on material surface.
Summary of the invention
In view of this, one of purpose of the present invention is to provide a kind of biological functional nanometer titanium material, by changing the topological structure and the chemical constituent of material surface, collaborative propagation and the differentiation capability that improves cell on titanium material surface, and then the bone conformability of raising titanium material.
For achieving the above object, the invention provides following technical scheme:
Biological functional nanometer titanium material, titanium material surface has the TiO that caliber is 30 ~ 100nm
2Nano-tube array, described TiO
2The nano-tube array surface also is fixed with bone morphogenetic protein 2(BMP2).
Further, described BMP2 is fixed on TiO by poly-dopamine
2The nano-tube array surface.
Two of purpose of the present invention is to provide a kind of preparation method of described biological functional nanometer titanium material, and is easy to operation, and the cycle is short, and cost is low.
For achieving the above object, the invention provides following technical scheme:
The preparation method of biological functional nanometer titanium material may further comprise the steps:
A, the titanium material is carried out pretreatment according to conventional method;
B, employing anodizing are the TiO of 30 ~ 100nm at the pretreated titanium material of step a surface preparation caliber
2Nano-tube array;
C, the TiO that makes at step b
2The nano-tube array surface deposition gathers dopamine;
D, make BMP2 be fixed on the TiO that surface deposition that step c makes has poly-dopamine by haptoreaction
2The nano-tube array surface promptly makes biological functional nanometer titanium material.
Further, described step a used acetone, dehydrated alcohol, deionized water ultrasonic cleaning 10 ~ 30 minutes successively with the titanium material, drying for standby.
Further, described step b is to be anode with the pretreated titanium material of step a, and platinum electrode is a negative electrode, put in the ammonium fluoride electrolyte, electrolysis under the constant voltage of 10 ~ 25V is with the titanium material deionized water wash after the oxidation, drying makes the surface and has the TiO that caliber is 30 ~ 100nm
2The titanium material of nano-tube array.
Further, described step b is to be that 50% dissolve with ethanol solution is made the solution that concentration is 0.27mol/L with the ammonium fluoride volume fraction, as electrolyte; Be anode with the pretreated titanium material of step a again, platinum electrode is a negative electrode, puts in the above-mentioned ammonium fluoride electrolyte, and electrolysis is 3 hours under the constant voltage of 10 ~ 25V, and with the titanium material deionized water wash after the oxidation, drying makes the surface and has the TiO that caliber is 30 ~ 100nm
2The titanium material of nano-pipe array thin film.
Further, described step c has TiO with the surface that step b makes
2The titanium material of nano-tube array is put in the alkaline dopamine solution and is reacted, and makes TiO
2The nano-tube array surface deposition has the titanium material of poly-dopamine.
Further, described step c is to be that the solution that concentration is 2mg/mL is made in the TRIS buffer dissolving of 10mmol/L with dopamine hydrochloride concentration, regulates pH to 8.5; The surface that step b is made has TiO
2The titanium material of nano-tube array immerses in the above-mentioned alkaline dopamine solution, and the lucifuge reaction is spent the night under the room temperature, the reuse deionized water wash, and drying makes TiO
2The nano-tube array surface deposition has the titanium material of poly-dopamine.
Further, described steps d is that BMP2 is made the solution that concentration is 80ng/mL with PBS dissolving, the TiO that step c is made
2The nano-tube array surface deposition has the titanium material of poly-dopamine to immerse in the above-mentioned BMP2 solution, and 4 ℃ of reactions are spent the night, reuse PBS washing, and drying promptly makes biological functional nanometer titanium material.
Beneficial effect of the present invention is: it is the TiO of 30 ~ 100nm that the present invention makes up caliber by anodizing on titanium material surface
2Nano-tube array is again by gathering dopamine at TiO
2The fixing BMP2 that goes up in nano-tube array surface has obtained a kind of biological functional nanometer titanium material; This titanium material has excellent biological compatibility, on the one hand, and the TiO of suitable caliber
2Nano-tube array can be the cell growth suitable topological structure is provided; On the other hand, BMP2 can promote cell differentiation as somatomedin; By the synergism of topological structure and chemical constituent, can improve propagation and the differentiation capability of cell, and then improve the bone conformability of titanium material on titanium material surface.In addition, the preparation method of this titanium material is simple and easy to do, and the cycle is short, and cost is low.Therefore, biological functional nanometer titanium material of the present invention has a good application prospect.
Description of drawings
Fig. 1 has the TiO that caliber is 30nm for the surface
2Field emission scanning electron microscope (FE-SEM) figure of the titanium material of nano-tube array.
Fig. 2 has the TiO that caliber is 60nm for the surface
2The FE-SEM figure of the titanium material of nano-tube array.
Fig. 3 has the TiO that caliber is 100nm for the surface
2The FE-SEM figure of the titanium material of nano-tube array.
Fig. 4 has TiO for the surface
2X-ray photoelectron power spectrum (XPS) figure on the titanium material surface of nano-tube array.
Fig. 5 is TiO
2The nano-tube array surface deposition has the XPS spectrum figure on the titanium material surface of poly-dopamine.
Fig. 6 has TiO for the surface
2The XPS spectrum figure on the titanium material surface that nano-tube array and BMP2 modify.
Fig. 7 is the ability of biological functional nanometer titanium material spatial induction rat bone marrow mesenchymal stem cells of the present invention (MSCs) to osteoblast differentiation.
The specific embodiment
In order to make the purpose, technical solutions and advantages of the present invention clearer, the preferred embodiments of the present invention are described in detail below in conjunction with accompanying drawing.
Embodiment 1, biological functional nanometer titanium material (TiO
2
The nanotube caliber is 30nm) preparation
May further comprise the steps:
A, the titanium material was used acetone, dehydrated alcohol, deionized water ultrasonic cleaning 20 minutes successively, 37 ℃ of oven dry, standby;
B, be that 50% dissolve with ethanol solution is made the solution that concentration is 0.27mol/L with the ammonium fluoride volume fraction, as electrolyte; Be anode with the pretreated titanium material of step a again, platinum electrode is a negative electrode, puts in the above-mentioned ammonium fluoride electrolyte, electrolysis is 3 hours under the unidirectional current effect of 10V, with deionized water washing by soaking 3 times of the titanium material after the oxidation, 37 ℃ of oven dry make the surface and have the TiO that caliber is 30nm
2The titanium material of nano-tube array, its FE-SEM figure is as shown in Figure 1;
C, be that the solution that concentration is 2mg/mL is made in the Tris buffer dissolving of 10mmol/L, regulate pH to 8.5 dopamine hydrochloride concentration; The surface that step b is made has TiO
2The titanium material of nano-tube array immerses in the above-mentioned alkaline dopamine solution, and the lucifuge reaction is spent the night under the room temperature, reuse deionized water wash 3 times, and natural drying makes TiO
2The nano-tube array surface deposition has the titanium material of poly-dopamine;
D, BMP2 is made the solution that concentration is 80ng/mL with PBS dissolving, the TiO that step c is made
2The nano-tube array surface deposition has the titanium material of poly-dopamine to immerse in the above-mentioned BMP2 solution, and 4 ℃ of reactions are spent the night, reuse PBS washing 3 times, and drying makes the surface and has TiO
2The titanium material that nano-tube array and BMP2 modify, biological functional nanometer titanium material (TiO promptly of the present invention
2The nanotube caliber is 30nm).
Get the titanium material that step b, c, d make respectively, its surface chemistry is formed carried out XPS and characterize, the result is shown in Fig. 4 ~ 6 and table 1, and visible N constituent content improves gradually, illustrates at TiO
2BMP2 has successfully been introduced on the nano-tube array surface.
The chemical composition of table 1, sample surfaces (by the quality percentage composition)
May further comprise the steps:
A, the titanium material was used acetone, dehydrated alcohol, deionized water ultrasonic cleaning 20 minutes successively, 37 ℃ of oven dry, standby;
B, be that 50% dissolve with ethanol solution is made the solution that concentration is 0.27mol/L with the ammonium fluoride volume fraction, as electrolyte; Be anode with the pretreated titanium material of step a again, platinum electrode is a negative electrode, puts in the above-mentioned ammonium fluoride electrolyte, electrolysis is 3 hours under the unidirectional current effect of 20V, with deionized water washing by soaking 3 times of the titanium material after the oxidation, 37 ℃ of oven dry make the surface and have the TiO that caliber is 60nm
2The titanium material of nano-tube array, its FE-SEM figure is as shown in Figure 2;
C, be that the solution that concentration is 2mg/mL is made in the Tris buffer dissolving of 10mmol/L, regulate pH to 8.5 dopamine hydrochloride concentration; The surface that step b is made has TiO
2The titanium material of nano-tube array immerses in the above-mentioned alkaline dopamine solution, and the lucifuge reaction is spent the night under the room temperature, reuse deionized water wash 3 times, and natural drying makes TiO
2The nano-tube array surface deposition has the titanium material of poly-dopamine;
D, BMP2 is made the solution that concentration is 80ng/mL with PBS dissolving, the TiO that step c is made
2The nano-tube array surface deposition has the titanium material of poly-dopamine to immerse in the above-mentioned BMP2 solution, and 4 ℃ of reactions are spent the night, reuse PBS washing 3 times, and drying makes the surface and has TiO
2The titanium material that nano-tube array and BMP2 modify, biological functional nanometer titanium material (TiO promptly of the present invention
2The nanotube caliber is 60nm).
May further comprise the steps:
A, the titanium material was used acetone, dehydrated alcohol, deionized water ultrasonic cleaning 20 minutes successively, 37 ℃ of oven dry, standby;
B, be that 50% dissolve with ethanol solution is made the solution that concentration is 0.27mol/L with the ammonium fluoride volume fraction, as electrolyte; Be anode with the pretreated titanium material of step a again, platinum electrode is a negative electrode, puts in the above-mentioned ammonium fluoride electrolyte, electrolysis is 3 hours under the unidirectional current effect of 25V, with deionized water washing by soaking 3 times of the titanium material after the oxidation, 37 ℃ of oven dry make the surface and have the TiO that caliber is 100nm
2The titanium material of nano-tube array, its FE-SEM figure is as shown in Figure 3;
C, be that the solution that concentration is 2mg/mL is made in the Tris buffer dissolving of 10mmol/L, regulate pH to 8.5 dopamine hydrochloride concentration; The surface that step b is made has TiO
2The titanium material of nano-tube array immerses in the above-mentioned alkaline dopamine solution, and the lucifuge reaction is spent the night under the room temperature, reuse deionized water wash 3 times, and natural drying makes TiO
2The nano-tube array surface deposition has the titanium material of poly-dopamine;
D, BMP2 is made the solution that concentration is 80ng/mL with PBS dissolving, the TiO that step c is made
2The nano-tube array surface deposition has the titanium material of poly-dopamine to immerse in the above-mentioned BMP2 solution, and 4 ℃ of reactions are spent the night, reuse PBS washing 3 times, and drying makes the surface and has TiO
2The titanium material that nano-tube array and BMP2 modify, biological functional nanometer titanium material (TiO promptly of the present invention
2The nanotube caliber is 100nm).
Adopt density to combine and cultivate rat marrow MSCs with adherent screening method.With 3 * 10
4Individual MSCs is inoculated into the rounded material that diameter is 1.5mm, and (the titanium material, the surface that are respectively surperficial untreated titanium material, surperficial BMP2 modification have the TiO that caliber is 30 ~ 100nm
2The biological functional nanometer titanium material that the titanium material of nano-tube array and embodiment 1 ~ 3 make) surface, with containing the DMEM culture medium culturing that volume fraction is 10% new-born calf serum, changed fresh culture in per 3 days, after cultivating 7 days and 14 days, with mass fraction is the digestion of 0.25% pancreatin solution, and collecting cell is 1% Triton-X100 solution cell lysis with mass fraction, the centrifuging and taking supernatant, the expression of detection lysis supernatant neutral and alkali phosphatase.
The result as shown in Figure 7, the untreated titanium material of titanium material phase specific surface that surface BMP2 modifies, the alkali phosphatase expression of the rat marrow MSCs of its surface seeding when cultivating 7 days, 14 days obviously improves (P＜0.05), illustrates that being introduced separately into BMP2 on titanium material surface can promote bone marrow MSCs to osteoblast differentiation; The surface has the TiO that caliber is 30nm
2The untreated titanium material of titanium material phase specific surface of nano-tube array, the alkali phosphatase expression of the rat marrow MSCs of its surface seeding when cultivating 7 days has certain increase, alkali phosphatase expression when cultivating 14 days is close, and the surface has the TiO that caliber is 100nm
2The untreated titanium material of titanium material phase specific surface of nano-tube array, the alkali phosphatase expression of the rat marrow MSCs of its surface seeding when cultivating 7 days, 14 days all reduces, and the TiO that is introduced separately into suitable caliber on titanium material surface is described
2Nano-tube array also can promote bone marrow MSCs to osteoblast differentiation in the early stage; TiO
2The nanotube caliber is that biological functional nanometer titanium material and the surface of 30 ~ 100nm has corresponding caliber TiO
2Alkali phosphatase expression difference between the titanium material of nano-tube array, cultivated 7 days or 14 days no matter be, all, illustrate on titanium material surface and introduce TiO simultaneously apparently higher than the titanium material of surperficial BMP2 modification and the alkali phosphatase expression difference (P＜0.05) between the surperficial untreated titanium material
2Nano-tube array structure and BMP2 can work in coordination with promotion rat marrow MSCs to osteoblast differentiation, wherein are again the TiO of 30nm with the caliber
2Nano-tube array is best.
Explanation is at last, above embodiment is only unrestricted in order to technical scheme of the present invention to be described, although by invention has been described with reference to the preferred embodiments of the present invention, but those of ordinary skill in the art is to be understood that, can make various changes to it in the form and details, and the spirit and scope of the present invention that do not depart from appended claims and limited.
Claims (9)
1. biological functional nanometer titanium material, it is characterized in that: titanium material surface has the TiO that caliber is 30 ~ 100nm
2Nano-tube array, described TiO
2It is BMP2 that the nano-tube array surface also is fixed with bone morphogenetic protein 2.
2. biological functional nanometer titanium material according to claim 1 is characterized in that: described BMP2 is fixed on TiO by poly-dopamine
2The nano-tube array surface.
3. the preparation method of claim 1 or 2 described biological functional nanometer titanium materials is characterized in that: may further comprise the steps:
A, the titanium material is carried out pretreatment according to conventional method;
B, employing anodizing are the TiO of 30 ~ 100nm at the pretreated titanium material of step a surface preparation caliber
2Nano-tube array;
C, the TiO that makes at step b
2The nano-tube array surface deposition gathers dopamine;
D, make BMP2 be fixed on the TiO that surface deposition that step c makes has poly-dopamine by haptoreaction
2The nano-tube array surface promptly makes biological functional nanometer titanium material.
4. the preparation method of biological functional nanometer titanium material according to claim 3 is characterized in that: described step a used acetone, dehydrated alcohol, deionized water ultrasonic cleaning 10 ~ 30 minutes successively with the titanium material, drying for standby.
5. the preparation method of biological functional nanometer titanium material according to claim 3, it is characterized in that: described step b is to be anode with the pretreated titanium material of step a, platinum electrode is a negative electrode, put in the ammonium fluoride electrolyte, electrolysis under the constant voltage of 10 ~ 25V, with the titanium material deionized water wash after the oxidation, drying makes the surface and has the TiO that caliber is 30 ~ 100nm
2The titanium material of nano-tube array.
6. the preparation method of biological functional nanometer titanium material according to claim 5 is characterized in that: described step b is to be that 50% dissolve with ethanol solution is made the solution that concentration is 0.27mol/L with the ammonium fluoride volume fraction, as electrolyte; Be anode with the pretreated titanium material of step a again, platinum electrode is a negative electrode, puts in the above-mentioned ammonium fluoride electrolyte, and electrolysis is 3 hours under the constant voltage of 10 ~ 25V, and with the titanium material deionized water wash after the oxidation, drying makes the surface and has the TiO that caliber is 30 ~ 100nm
2The titanium material of nano-pipe array thin film.
7. the preparation method of biological functional nanometer titanium material according to claim 3 is characterized in that: described step c has TiO with the surface that step b makes
2The titanium material of nano-tube array is put in the alkaline dopamine solution and is reacted, and makes TiO
2The nano-tube array surface deposition has the titanium material of poly-dopamine.
8. the preparation method of biological functional nanometer titanium material according to claim 7, it is characterized in that: described step c is to be that the solution that concentration is 2mg/mL is made in the TRIS buffer dissolving of 10mmol/L with dopamine hydrochloride concentration, regulates pH to 8.5; The surface that step b is made has TiO
2The titanium material of nano-tube array immerses in the above-mentioned alkaline dopamine solution, and the lucifuge reaction is spent the night under the room temperature, the reuse deionized water wash, and drying makes TiO
2The nano-tube array surface deposition has the titanium material of poly-dopamine.
9. the preparation method of biological functional nanometer titanium material according to claim 3 is characterized in that: described steps d is that BMP2 is made the solution that concentration is 80ng/mL with PBS dissolving, the TiO that step c is made
2The nano-tube array surface deposition has the titanium material of poly-dopamine to immerse in the above-mentioned BMP2 solution, and 4 ℃ of reactions are spent the night, reuse PBS washing, and drying promptly makes biological functional nanometer titanium material.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2010106185709A CN102058904A (en) | 2010-12-31 | 2010-12-31 | Biological functionalized nano titanium material and preparation method thereof |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2010106185709A CN102058904A (en) | 2010-12-31 | 2010-12-31 | Biological functionalized nano titanium material and preparation method thereof |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN102058904A true CN102058904A (en) | 2011-05-18 |
Family
ID=43994524
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN2010106185709A Pending CN102058904A (en) | 2010-12-31 | 2010-12-31 | Biological functionalized nano titanium material and preparation method thereof |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN102058904A (en) |
Cited By (18)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102560598A (en) * | 2012-01-18 | 2012-07-11 | 重庆大学 | Method for preparing medical titanium material with high anti-cancer and antibacterial properties |
| CN102614783A (en) * | 2012-03-27 | 2012-08-01 | 大连理工大学 | Method for preparing high-flux composite membrane from dopamine-modified nanometer material |
| CN102813963A (en) * | 2012-09-10 | 2012-12-12 | 高长有 | Method for fixing functional molecules on biomedical material surface with dopamine serving as bridging |
| CN103357070A (en) * | 2013-07-26 | 2013-10-23 | 河北工业大学 | Medical beta-titanium alloy composite material with osteogenesis inducing activity and preparation method thereof |
| CN104005016A (en) * | 2014-06-06 | 2014-08-27 | 重庆大学 | Medical titanium alloy with antibacterial and osteocyte-facilitating functions and preparation method thereof |
| CN106521601A (en) * | 2016-11-22 | 2017-03-22 | 佳木斯大学 | Preparation method for micro-arc oxidation-dopamine coupling carried traditional Chinese medicine coating of pure titanium oral implant |
| CN106676607A (en) * | 2016-12-02 | 2017-05-17 | 江苏师范大学 | Titanium dioxide nanotube with good osteogenesis promoting function and preparation method thereof |
| CN106729964A (en) * | 2016-12-02 | 2017-05-31 | 江苏师范大学 | It is a kind of to have promoting bone growing concurrently and suppress titania nanotube of bone resorption and preparation method thereof |
| CN107029304A (en) * | 2016-10-24 | 2017-08-11 | 北京大学口腔医院 | Biological planting body and the method that active factors is prepared on matrix |
| CN107034508A (en) * | 2017-03-22 | 2017-08-11 | 浙江科惠医疗器械股份有限公司 | A kind of preparation method of UV auxiliary electrostatics atomization formation system and coating of titanium dioxide |
| CN107715172A (en) * | 2017-11-08 | 2018-02-23 | 江苏华友装饰工程有限公司 | A kind of preparation method of bone bionic composite material |
| CN108939156A (en) * | 2018-06-15 | 2018-12-07 | 南京冬尚生物科技有限公司 | 3D printing Ti-PDA-BMP-2 bone defect healing tissue engineering bracket and preparation method thereof |
| CN109381744A (en) * | 2018-09-14 | 2019-02-26 | 广州润虹医药科技股份有限公司 | A kind of calcium orthophosphate base bone repairing support and preparation method thereof |
| CN111759751A (en) * | 2017-08-03 | 2020-10-13 | 南昌大学 | Whitening toothpaste excited by blue light LED and preparation method thereof |
| CN112972759A (en) * | 2020-12-31 | 2021-06-18 | 华南理工大学 | Composite material for regulating and controlling surface bioactivity of material in vitro by using magnetic field and preparation method and application thereof |
| CN114346441A (en) * | 2022-01-21 | 2022-04-15 | 中国人民解放军总医院第一医学中心 | Surface treatment method for dental implant |
| CN114432500A (en) * | 2022-01-20 | 2022-05-06 | 南京医科大学附属口腔医院 | CeO (CeO)2Modified TiO2Bionic porous titanium stent of variable nanotube array and preparation method thereof |
| CN116920173A (en) * | 2023-06-27 | 2023-10-24 | 首都医科大学附属北京口腔医院 | PDA-D-amino-TNT composite coating for dental implant and preparation method thereof |
Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101411893A (en) * | 2008-11-20 | 2009-04-22 | 重庆大学 | Titanium or titanium alloy material with modified surface as well as preparation method and use thereof |
-
2010
- 2010-12-31 CN CN2010106185709A patent/CN102058904A/en active Pending
Patent Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101411893A (en) * | 2008-11-20 | 2009-04-22 | 重庆大学 | Titanium or titanium alloy material with modified surface as well as preparation method and use thereof |
Non-Patent Citations (2)
| Title |
|---|
| CHYE KHOON POH，ET AL: "The effect of VEGF functionalization of titanium on endothelial cells in vitro", 《BIOMATERIALS》, vol. 31, no. 7, 5 December 2009 (2009-12-05), pages 1578 - 1585, XP002664001, DOI: doi:10.1016/J.BIOMATERIALS.2009.11.042 * |
| GANESAN BALASUNDARAM ET A: "TiO2 nanotubes functionalized with regions of bone morphogenetic protein-2 increases osteoblast adhesion", 《JOURNAL OF BIOMEDICAL MATERIALS RESEARCH PART A》, vol. 84, no. 2, 6 July 2007 (2007-07-06), pages 447 - 453 * |
Cited By (22)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102560598A (en) * | 2012-01-18 | 2012-07-11 | 重庆大学 | Method for preparing medical titanium material with high anti-cancer and antibacterial properties |
| CN102614783A (en) * | 2012-03-27 | 2012-08-01 | 大连理工大学 | Method for preparing high-flux composite membrane from dopamine-modified nanometer material |
| CN102813963A (en) * | 2012-09-10 | 2012-12-12 | 高长有 | Method for fixing functional molecules on biomedical material surface with dopamine serving as bridging |
| CN103357070A (en) * | 2013-07-26 | 2013-10-23 | 河北工业大学 | Medical beta-titanium alloy composite material with osteogenesis inducing activity and preparation method thereof |
| CN104005016A (en) * | 2014-06-06 | 2014-08-27 | 重庆大学 | Medical titanium alloy with antibacterial and osteocyte-facilitating functions and preparation method thereof |
| CN107029304A (en) * | 2016-10-24 | 2017-08-11 | 北京大学口腔医院 | Biological planting body and the method that active factors is prepared on matrix |
| CN106521601A (en) * | 2016-11-22 | 2017-03-22 | 佳木斯大学 | Preparation method for micro-arc oxidation-dopamine coupling carried traditional Chinese medicine coating of pure titanium oral implant |
| CN106676607A (en) * | 2016-12-02 | 2017-05-17 | 江苏师范大学 | Titanium dioxide nanotube with good osteogenesis promoting function and preparation method thereof |
| CN106729964A (en) * | 2016-12-02 | 2017-05-31 | 江苏师范大学 | It is a kind of to have promoting bone growing concurrently and suppress titania nanotube of bone resorption and preparation method thereof |
| CN107034508B (en) * | 2017-03-22 | 2019-01-25 | 浙江科惠医疗器械股份有限公司 | A kind of UV auxiliary electrostatic is atomized the preparation method of formation system and coating of titanium dioxide |
| CN107034508A (en) * | 2017-03-22 | 2017-08-11 | 浙江科惠医疗器械股份有限公司 | A kind of preparation method of UV auxiliary electrostatics atomization formation system and coating of titanium dioxide |
| CN111759751A (en) * | 2017-08-03 | 2020-10-13 | 南昌大学 | Whitening toothpaste excited by blue light LED and preparation method thereof |
| CN107715172A (en) * | 2017-11-08 | 2018-02-23 | 江苏华友装饰工程有限公司 | A kind of preparation method of bone bionic composite material |
| CN108939156A (en) * | 2018-06-15 | 2018-12-07 | 南京冬尚生物科技有限公司 | 3D printing Ti-PDA-BMP-2 bone defect healing tissue engineering bracket and preparation method thereof |
| CN108939156B (en) * | 2018-06-15 | 2020-12-08 | 南京冬尚生物科技有限公司 | 3D printing Ti-PDA-BMP-2 bone defect repair tissue engineering scaffold and preparation method thereof |
| CN109381744A (en) * | 2018-09-14 | 2019-02-26 | 广州润虹医药科技股份有限公司 | A kind of calcium orthophosphate base bone repairing support and preparation method thereof |
| CN112972759A (en) * | 2020-12-31 | 2021-06-18 | 华南理工大学 | Composite material for regulating and controlling surface bioactivity of material in vitro by using magnetic field and preparation method and application thereof |
| CN114432500A (en) * | 2022-01-20 | 2022-05-06 | 南京医科大学附属口腔医院 | CeO (CeO)2Modified TiO2Bionic porous titanium stent of variable nanotube array and preparation method thereof |
| CN114432500B (en) * | 2022-01-20 | 2022-09-16 | 南京医科大学附属口腔医院 | CeO (CeO) 2 Modified TiO 2 2 Bionic porous titanium stent of variable nanotube array and preparation method thereof |
| CN114346441A (en) * | 2022-01-21 | 2022-04-15 | 中国人民解放军总医院第一医学中心 | Surface treatment method for dental implant |
| CN116920173A (en) * | 2023-06-27 | 2023-10-24 | 首都医科大学附属北京口腔医院 | PDA-D-amino-TNT composite coating for dental implant and preparation method thereof |
| CN116920173B (en) * | 2023-06-27 | 2024-03-08 | 首都医科大学附属北京口腔医院 | PDA-D-amino-TNT composite coating for dental implant and preparation method thereof |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN102058904A (en) | Biological functionalized nano titanium material and preparation method thereof | |
| CN102560598B (en) | Method for preparing medical titanium material with high anti-cancer and antibacterial properties | |
| CN107304472B (en) | Have both the medical titanium-based composite coat and preparation method thereof of Bone Defect Repari function and anti-microbial property | |
| CN102058901B (en) | Surface functional titanium material and preparation method thereof | |
| CN109267137B (en) | Preparation method of medical titanium surface gradient nano silver | |
| Shi et al. | An effective self-powered strategy to endow titanium implant surface with associated activity of anti-biofilm and osteogenesis | |
| CN106474546B (en) | A kind of electric polypyrrole/poly-dopamine nanofiber and the preparation method and application thereof | |
| CN102525827B (en) | Method for preparing medical titanium material with long-acting antibacterial property and good biocompatibility | |
| CN103736148B (en) | The method preparing titanium implant and the titanium implant obtained | |
| CN102743789B (en) | Artificial tooth root with micro-nano hierarchical topologic surface structure and preparation method of artificial tooth root | |
| CN106676607A (en) | Titanium dioxide nanotube with good osteogenesis promoting function and preparation method thereof | |
| CN107661544A (en) | Antibacterial facilitates porous orthopaedics implant of bone complex function and preparation method thereof | |
| CN107761148B (en) | A method of fibroin albumen hydroxyapatite coating layer is prepared in metal surface | |
| CN108815571B (en) | Preparation method of silver modified crystal form titanium dioxide nanotube layer | |
| CN106902384A (en) | A kind of method for preparing class bone structure film layer on titanium surface | |
| CN106729964A (en) | It is a kind of to have promoting bone growing concurrently and suppress titania nanotube of bone resorption and preparation method thereof | |
| CN106637310B (en) | A kind of Cu/PAA composite membrane and preparation method thereof and application | |
| CN108912375A (en) | The oriented growth nanogold bacteria cellulose/compound film method of carbon pipe | |
| CN105803428A (en) | Method for preparing zinc-doped nanometer structure with bacteriostatic action on surface of porous pure titanium | |
| CN105030353A (en) | Preparation method of dental implant of multistage nano morphologic structure | |
| CN110292654A (en) | A kind of titanium alloy surface collagenic coating and preparation method thereof loading antibacterial polypeptide | |
| CN105331946B (en) | A kind of method that zirconium-ion implantation improves medical polyetheretherketonematerials materials bioactivity | |
| CN107648674A (en) | With antibacterial and the metal implant for promoting Integrated implant function and preparation method thereof | |
| CN103536371B (en) | A kind of dentistry implant and preparation method thereof | |
| Guette-Marquet et al. | The electrochemical potential is a key parameter for cell adhesion and proliferation on carbon surface |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| C12 | Rejection of a patent application after its publication | ||
| RJ01 | Rejection of invention patent application after publication |
Application publication date: 20110518 |