CN108937849A - One kind indicating system for the imaging of tumour nano target fluorescence probe and surgical navigational - Google Patents
One kind indicating system for the imaging of tumour nano target fluorescence probe and surgical navigational Download PDFInfo
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- CN108937849A CN108937849A CN201710394518.1A CN201710394518A CN108937849A CN 108937849 A CN108937849 A CN 108937849A CN 201710394518 A CN201710394518 A CN 201710394518A CN 108937849 A CN108937849 A CN 108937849A
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61B—DIAGNOSIS; SURGERY; IDENTIFICATION
- A61B5/00—Measuring for diagnostic purposes; Identification of persons
- A61B5/0059—Measuring for diagnostic purposes; Identification of persons using light, e.g. diagnosis by transillumination, diascopy, fluorescence
- A61B5/0075—Measuring for diagnostic purposes; Identification of persons using light, e.g. diagnosis by transillumination, diascopy, fluorescence by spectroscopy, i.e. measuring spectra, e.g. Raman spectroscopy, infrared absorption spectroscopy
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61B—DIAGNOSIS; SURGERY; IDENTIFICATION
- A61B5/00—Measuring for diagnostic purposes; Identification of persons
- A61B5/0059—Measuring for diagnostic purposes; Identification of persons using light, e.g. diagnosis by transillumination, diascopy, fluorescence
- A61B5/0071—Measuring for diagnostic purposes; Identification of persons using light, e.g. diagnosis by transillumination, diascopy, fluorescence by measuring fluorescence emission
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61B—DIAGNOSIS; SURGERY; IDENTIFICATION
- A61B2503/00—Evaluating a particular growth phase or type of persons or animals
- A61B2503/40—Animals
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61B—DIAGNOSIS; SURGERY; IDENTIFICATION
- A61B2503/00—Evaluating a particular growth phase or type of persons or animals
- A61B2503/42—Evaluating a particular growth phase or type of persons or animals for laboratory research
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Abstract
One kind can help operative doctor to judge position existing for tumour cell for the imaging of tumour nano target fluorescence probe and operation guiding system.The optics indicate system by near infrared imaging object lens, CCD receiver, three D eyepieces and high power is micro- shows that device is constituted.By the near infrared light of 1325nm tumour specific region surface, tumour cell is imaged on CCD by near infrared imaging system, the information of collection is transferred to computer by CCD, it is 580nm micro-display that operation wavelength is transmitted to after computer enhancement information, the tumour cell image solid that final micro-display will be seen that by three D eyepieces is presented in the visual field of doctor, the tumor image and actual knub position with it is equal in magnitude.The systemic resolution is up to 1024 x768, and compared with existing tumour display system, not only Ju has that Jie Gou Jian Unit is compact, numerical aperture is big, high resolution and the features such as light and smallization.Furthermore, it is possible to observe the solid shape and specific location of cancer cell, position and boundary existing for tumour is accurately positioned in the information that doctor can provide according to instruction system.
Description
Technical field
The invention belongs to optical imagery instrument and diagnosis cancer field, be related to it is a kind of for tumour and nano target fluorescence probe at
Picture and surgical navigational indicate system.
Background technique
Many development of molecular imaging have made people in vivo special molecular action target spot and molecular action path
There is the understanding of more intuitive image.In recent years, the trend of diversified development was presented in the mode of high-resolution in-vivo imaging, main to wrap
It includes: radionuclide imaging, such as positron emission tomography (PET) and single photon emission tomography
(SPE CT), magnetic resonance imaging (MRI) and optical imagery (optical im aging).In its optical imaging field
Emerging near-infrared fluorescent (near-infrared f luorescence, N IR F) is imaged gradually by stakeholder
Attention.The new development of near-infrared fluorescence imaging mainly includes the exploitation of the good near infrared fluorescent dye of bio-compatibility and and each
The synthesis of class probe, and for the experimental research that various disease animal models are carried out.The original substantially of near-infrared fluorescence imaging
Reason is to irradiate fluorescent molecule (near infrared fluorescent probe) with the excitation light source of specific spectra range, and fluorescent molecule is inspired at this time
The photon signal of different spectral characteristics, this signal are shone after passing through optical filter by super quick CCD (charge-coupleddevice)
Camera acquisition, is then converted to image for photon signal by high-level data processing technique.In recent years the development master of NIRF imaging
To be the synthesis of various special target probes and clever probe, and be applied to the experimental research of various disease, and is main
It concentrates on the early diagnosis of tumour, inflammation and cardiovascular disease and treats the dynamic monitoring of curative effect, be disease from molecular level
Occur, develop, offer life-information is provided.
As nir instrument is in biomedical development, applies and have become new trend in diagnosing tumor and therapy field.
Near-infrared fluorescence imaging technology becomes expression of gene protein and various cellular activities in Noninvasive observation living animal body
It may.Relative to high-field magnetic resonance, other Noninvasive Observations Means such as radionuclide imaging, the light of near-infrared fluorescence imaging
Son amount detection thresholding is low, therefore detection sensitivity is high；Each probe is to design for some specific molecular target, therefore specific
By force.Currently, near-infrared fluorescence imaging is still within cell in vitro research and animal experiment study stage, and by increasingly wider
It is applied to medicine and biological study field generally.With the continuous development of new technology and continuing to bring out for new equipment, near-infrared
The research range of fluorescence imaging will constantly expand, and provide kind of a brand-new method for diagnosing human disease and treatment.It is raw in the area NIR
The absorption and transmitting of object and its tissue are all very weak, thus are referred to as " Biological Window ", the low back in the region
Scape fluorescence also can increase the sensitivity of fluorescent technique.Since light scattering of the organism to near-infrared fluorescent is also very weak, also make
Near infrared light can penetrate deeper in vivo, and according to the literature up to 12cm, these features are but also NIR fluorescent technique
Application in bio-imaging field is very tempting.But traditional fluoroscopic imaging systems penetration depth is low, signal-to-noise ratio is not high, seriously
Constrain application of the Imaging-PAM in biomedicine.Since mostly concentrate on can for traditional fluorescent molecular probe luminous
Light-exposed range (400-750nm), and many organisms and its group be woven under the excitation of ultraviolet/visible light itself can emit it is glimmering
Light, thus the fluorescence detection and imaging of severe jamming biological sample.Using II area's light (1000~1350nm) of near-infrared in life
The feature high with imaging signal to noise ratio is penetrated in object tissue, using the system, there can be near-infrared fluorescent spy to label
Biomolecule, living cells, the organ of living small animal and tissue (including lymphatic system, blood vessel and tumour) of needle etc. carry out at
Picture, can observe gene in the intracorporal expression of living small animal in real time in this way, the generation of tumour, growth, transfer and drug
Therapeutic effect.
In recent years, the second near-infrared window (NIR-II, 1000-1350 nm) fluorescent material is concerned.Therefore, second is close
Infrared window fluorescent material compares conventional fluorescent material with greater advantage in terms of biological detection and living imaging.Theoretically
Its quantum dot fluorescence emission peak covers the second infrared window.With the second infrared window fluorescence quantum CdHgTe before,
PbS, PbSe and PbTe etc. are different, and Ag E quantum dot is without containing toxic heavy-metal elements such as cd, Pb or Hg, more suitable for work
Body imaging has been reported that: using sulfydryl octane as surface ligand, by regulating and controlling the ratio of Ag precursor and Te precursor, being prepared for second
Infrared window fluorescence Ag2The oil-soluble Ag of uniform particle diameter, good dispersion has been made in Te quantum dot2Te quantum dot, it is maximum
Fluorescence emission wavelengths are located at 1325nm, and fluorescence quantum yield is up to 4.2%.Moreover, also in further improvement, this is received
Rice technology, so that Ag2Te can bonding probes, can go directly to tumour cell, be on the molecular level for detecting tumour
Eucaryotic cell structure.Doctor can monitor the targeted nano fluorescence in tumour by monitoring net using this nanotechnology, to sentence
The degree of danger of disconnected tumour and distribution and boundary, health tissues can also be monitored at any time at the same time, can accurately determine hand
Art range.A kind of in-vivo monitoring system for monitoring cancer and diagnosing the illness is developed by means of infrared imaging and nanotechnology
System is combined using nanoparticle with molecule sensor to detect the effect and variation of intracorporal chemistry or biomolecule, to examine
Occurrence and development process that is disconnected and judging tumour.So this system is expected to the early diagnosis in disease, drug development and analysis life
It is widely applied in the fields such as mechanism.
Summary of the invention
The purpose of the present invention is overcome current cancer diagnosis can only find 1mm or more cancer lesion detection means
(PET-CT, MRI) is insufficient, provides one kind for the imaging of tumour nano target fluorescence probe and surgical navigational instruction system, with standard
The position for really judging 1mm or less tumour cell, greatly improves the success rate of early diagnosis of cancer and operation.
The present invention provides one kind for the imaging of tumour nano target fluorescence probe and surgical navigational instruction system, comprising: raw
Object sensor, CCD receiver, near infrared imaging object lens, micro- indicator and the three-dimensional eyepiece operation real-time navigation instruction system of three D
System.Cancer cell is imaged in CCD receiver, CCD receiver and micro-display with the near infrared imaging object lens and computer connects
It connects, the tumour cell and boundary image that micro- indicator will be seen that by three D eyepieces are presented in the doctor visual field.
Described one kind is imaged for tumour nano target fluorescence probe and surgical navigational instruction system is to pass through 1325nm
Near infrared light specific region tumor surface, near infrared imaging object lens are by the area specific 64mm x48mm of 320mm object distance
Tumor imaging in domain is on CCD, and the information of collection is transferred to computer by data line by CCD, after computer enhancement information
Being transmitted to operation wavelength by data line is 580nm micro-display, and final micro-display will be seen that by the three-dimensional eyepiece of three D
Tumour cell image is looked up in angle stereoscopic projections to the visual field of doctor with 18 ° of maximum.The three-dimensional eyepiece of three D goes out
Pupil diameter is 12mm, and distance of exit pupil 60mm, maximum field of view angle is 18 °；The effective coverage of the detector of the CCD receiver is
(area is 8.38mm X6.89mm, and resolution ratio is EGA(-1024 X768, and pixel dimension is 8.25 μm 8.25 μm of X, image planes
Resolution ratio is determined by the nyquist frequency of CCD :=1000/2a, wherein for Nai Kuixin distinct frequence, i.e. image planes resolution ratio a
For one-dimensional pixel dimension ,=107 (1p/mm) are calculated；The near infrared imaging object lens hereby cut down lens by six chips
Composition, the radius of curvature of first lens is respectively 16.36mm and 60.36mm；The radius of curvature of second lens be respectively-
29.32mm with -36.36mm；The radius of curvature of third piece lens is respectively 22.35mm and -46,17mm；4th lens
Radius of curvature is respectively -10,45mm and 61.12mm, while the convex surface of first lens is secondary aspherical, two-term coefficient
It is -0. 56；The radius of curvature of 5th lens is respectively 12.76mm and 50.66mm；The radius of curvature of 6th the first lens
Respectively -16.36mm and -59.36mm.The effective coverage of the micro-display is 12.37mm X 9.48mm, and resolution ratio is
Extended Graphics Array-1024 X768, operation wavelength 580nm, nyquist frequency 1269p/mm.It is described
Penetration eyepiece by a piece of convex lens, a piece of double glued mirrors and semi-transparent semi-reflecting lens composition, the radius of curvature of convex lens are respectively
23.19mm and -92.35mm, the face that convex lens radius of curvature is 22.98mm are secondary aspherical, and two-term coefficient is -0.81, double
The radius of curvature of glued mirror is respectively 20.43mm and -281.36mm and 13.42mm, and semi-transparent semi-reflecting lens are located on emitting light path,
And with optical path at 45 ° of angles, reflecting surface is towards doctor's eyes.
Advantages of the present invention and special-effect
1. finding that II area's light (1000~1350nm) of near-infrared is stronger to the penetration capacity of biological tissue, imaging signal to noise ratio is more
Height, imaging technique is clear in biological living tumor imaging, is higher by existing conventional method；
2. near infrared imaging object lens have the large-numerical aperture of F/2.6, it is conducive to collect weak near-infrared reflection light imaging；
3. light only once passes through half-transmitting and half-reflecting mirror in systems, energy loss is low；
4. doctor can observe clearly tumour cell image simultaneously, and obtain more true solid space experience.
Detailed description of the invention
Fig. 1 is display this patent System Working Principle schematic diagram.
Fig. 2 is near-infrared fluorescent detector system structural schematic diagram.
Fig. 3 " tissue window " near infrared ray penetrates the wave band of tissue.
Fig. 4 is Ag2Te nano-fluorescent grain antibody image.
Fig. 5 is after Rats With Hepatoma injects targeted nano fluorescence antibody, with the schematic diagram of infrared radiation.
Fig. 6 is the cancer cell that near-infrared (1325nm) and nano target fluorescence probe are imaged.
Fig. 7 is near-infrared object lens and goggle structure parameter list.
Specific embodiment
As shown in Figure 1, a kind of to indicate system for the imaging of tumour nano target fluorescence probe and surgical navigational: A, laser is sent out
Raw device B, magnetic suspension accelerator C, targeting target (tumor tissues) D, zoom eyepiece E, signal sensor F, magnification at high multiple device
G, signal picker H, central processing unit calculate center I, display.Semiconductor laser uses central wavelength for 1325nm's
Semiconductor laser, the laser that it is issued are projected to detection object by laser fiber group, since injection has fluorescent material, through exciting
After can generate fluorescence, fluorescence is identified by detector, the fluorescence issued and be converted to digital signal pass through signal transmission line pass
It transports in computer system and is handled, and finally realize imaging.
Fluorescence detection optical circuit path includes: the generation of light, from different perspectives observed tumor focus.
Detector includes: that objective with variable focal length (12), tomoscan (13), grating (18), zoom lens's () 19, dispersion are repaired
Positive device (20), varifocal reflecting mirror (23), fiber-optical probe (21), optical fiber probe (22), high power display (15), CCD(16),
Lens barrel tail end is additionally provided with laser fiber group and signal transmssion line way outlet, convenient in sets of lines.
Specific embodiment:
Embodiment 1
By synthesized water-soluble Ag2Te nanoparticle mixed with liver cancer antibody (Anti-MMP13 antibody (ab39012)) and from
The heart, precipitating are resuspended in PBS solution (37 DEG C of oscillation 30min), and the ultimate density that gained tumor marker injects solution is 3.6mg/
Ml, pH value are 7. 2.
Wistar mouse system, SD rat (6 week old, 350g or so) be used as experimental animal, make liver cancer model, determine at
After function, is anaesthetized than appropriate solution of receiving by 30mg/kg with 2% penta, cut off chesk hair and take dorsal position.With the extraction of 1ml syringe
0.5ml hepatic carcinoma marks injection softly to massage injection site after injection in hepatic vein inserting needle.5-10 minutes after injection,
It is irradiated, is acquired with Portable fluorescence detector glimmering in liver region with semiconductor laser (central wavelength 1325nm)
Optical signal.As a result: under 1325nm laser irradiation, liver detects hyperfluorescence signal, obvious with all state tissue contrasts, and can be clear
Clear imaging.
Embodiment 2
By synthesized water-soluble Ag2Te nanoparticle mixes liver cancer antibody and liver cancer antibody (Anti-MMP13 antibody
(ab39012)) it mixing and is centrifuged, precipitating is resuspended in PBS solution (37 DEG C of oscillation 30min), ultimate density 3.6mg/m1,
PH value is 7. 4.
Wistar mouse system, SD rat (6 week old, 350g or so) are used as experimental animal.Fluorescence nano is targeted in liver injection
The PBS solution 200u1 of particle is in hepatic vein blood vessel, with semiconductor laser (central wavelength 1325nm), in intravascular injection
Position is irradiated, and acquires fluorescence signal with portable up-conversion fluorescence detector.As a result: if using common exciting light
Be irradiated, can generate it is very strong reflection and white fluorescence, using upper 1325nm irradiation after carry out imaging can effectively avoid it is white
Fluorescence generates.
Claims (7)
1. one kind indicates system for the imaging of tumour nano target fluorescence probe and surgical navigational, it is characterised in that the optics
System includes: biosensor, CCD receiver, near infrared imaging object lens, micro- indicator and 3D stereo eye lens；It is described close red
Tumour cell is imaged in CCD receiver, CCD receiver and micro- indicator by biosensor and calculated by outer image-forming objective lens
Machine connection, micro- indicator will be seen that tumor image projects the visual field into operative doctor by three D stereo eye lens.
2. one kind according to claim 1 indicates system for the imaging of tumour nano target fluorescence probe and surgical navigational,
It is characterized in that the effective feeling of the detector of the CCD receiver is 8.38mm X6. 89mm, resolution ratio EGA by area
(Extended Graphics Array) -1024 X768, pixel dimension be 8.25 μm 8.25 μm of X, image planes resolution ratio by
The nyquist frequency of CCD determines :=1000/2 α, wherein that is, image planes resolution ratio, ɑ are one-dimensional picture for nyquist frequency
Elemental size calculates=128 (1p/mm).
3. one kind according to claim 1 indicates system for the imaging of tumour nano target fluorescence probe and surgical navigational,
It is formed it is characterized in that the near infrared imaging object lens hereby cut down lens by six chips, the radius of curvature difference of first lens
For 16.36mm and 60.36mm；The radius of curvature of second lens is respectively -29.32mm and -36.36mm；Third piece lens
Radius of curvature is respectively 22.35mm and -46.17mm；The radius of curvature of 4th lens is respectively -10.45mm and 61.12mm,
The convex surface of first lens is secondary aspherical simultaneously, and two-term coefficient is -0.56；The radius of curvature difference of 5th lens
For 12.76mm and 50.66mm；The radius of curvature of 6th the first lens is respectively -16.36mm and -59.36mm.
4. one kind according to claim 1 indicates system for the imaging of tumour nano target fluorescence probe and surgical navigational,
It is characterized in that the near infrared imaging object lens can to 380mm object distance the effective coverage 89mm X68mm imaging.
5. one kind according to claim 1 is for tumour and the imaging of nano target fluorescence probe and surgical navigational instruction system
System, it is characterised in that the effective coverage of the micro-display is 12.37mm X 9.48mm, resolution ratio EGA-1024
X768, operation wavelength 580nm, nyquist frequency 1269p/mm.
6. one kind according to claim 1 indicates system for the imaging of tumour nano target fluorescence probe and surgical navigational,
It is characterized in that the exit pupil diameter of the three-dimensional eyepiece of three D is 12mm, distance of exit pupil 60mm, maximum field of view angle is 18 °.
7. according to any one of claims 1 to 6 a kind of for the imaging of tumour nano target fluorescence probe and surgical navigational
Instruction system, it is characterised in that the optical system passes through the tumor surface of the near infrared light specific region of 1325nm,
Cancer cell in specific region is imaged on CCD by near infrared imaging object lens, and CCD transmits the information of collection by data line
To computer, computer is 580nm micro-display, final micro display by operation wavelength is transmitted to by data line after information enhancement
Device projects the lining of the doctor visual field at emergent pupil by the tumour cell image that the three-dimensional eyepiece of three D will be seen that.
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Citations (12)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102893137A (en) * | 2010-03-17 | 2013-01-23 | 曾海山 | Rapid multi-spectral imaging methods and apparatus and applications for cancer detection and localization |
| CN103385698A (en) * | 2012-08-09 | 2013-11-13 | 中国科学院苏州纳米技术与纳米仿生研究所 | Fluorescence imaging system and application thereof |
| US20140081133A1 (en) * | 2009-12-15 | 2014-03-20 | Emory University | System and methods for providing real-time anatomical guidance in a diagnostic or therapeutic procedure |
| US20140114181A1 (en) * | 2012-10-22 | 2014-04-24 | Research Foundation Of The City University Of New York | Time reversal optical tomography for locating targets in a highly scattering turbid medium |
| CN104007533A (en) * | 2014-05-21 | 2014-08-27 | 苏州莱能士光电科技有限公司 | Large-image-plane, high-resolution and low-distortion optical lens |
| CN104582622A (en) * | 2012-04-16 | 2015-04-29 | 儿童国家医疗中心 | Dual-mode stereo imaging system for tracking and control in surgical and interventional procedures |
| CN104586361A (en) * | 2015-01-08 | 2015-05-06 | 汇佳生物仪器（上海）有限公司 | Movable near-infrared imaging device |
| JP3208259U (en) * | 2015-10-29 | 2016-12-28 | アップル インコーポレイテッド | Near-infrared imaging lens |
| CN106483665A (en) * | 2016-12-28 | 2017-03-08 | 南开大学 | Eyepiece formula wears vein display optical system |
| CN106572792A (en) * | 2014-06-05 | 2017-04-19 | 海德堡大学 | Method and means for multispectral imaging |
| CN106596497A (en) * | 2017-01-16 | 2017-04-26 | 浙江大学 | Shortwave infrared fluorescence microimaging method |
| CN106610518A (en) * | 2015-10-23 | 2017-05-03 | 大立光电股份有限公司 | Image capture lens group, image taking device and electronic device |
-
2017
- 2017-05-29 CN CN201710394518.1A patent/CN108937849A/en active Pending
Patent Citations (12)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20140081133A1 (en) * | 2009-12-15 | 2014-03-20 | Emory University | System and methods for providing real-time anatomical guidance in a diagnostic or therapeutic procedure |
| CN102893137A (en) * | 2010-03-17 | 2013-01-23 | 曾海山 | Rapid multi-spectral imaging methods and apparatus and applications for cancer detection and localization |
| CN104582622A (en) * | 2012-04-16 | 2015-04-29 | 儿童国家医疗中心 | Dual-mode stereo imaging system for tracking and control in surgical and interventional procedures |
| CN103385698A (en) * | 2012-08-09 | 2013-11-13 | 中国科学院苏州纳米技术与纳米仿生研究所 | Fluorescence imaging system and application thereof |
| US20140114181A1 (en) * | 2012-10-22 | 2014-04-24 | Research Foundation Of The City University Of New York | Time reversal optical tomography for locating targets in a highly scattering turbid medium |
| CN104007533A (en) * | 2014-05-21 | 2014-08-27 | 苏州莱能士光电科技有限公司 | Large-image-plane, high-resolution and low-distortion optical lens |
| CN106572792A (en) * | 2014-06-05 | 2017-04-19 | 海德堡大学 | Method and means for multispectral imaging |
| CN104586361A (en) * | 2015-01-08 | 2015-05-06 | 汇佳生物仪器（上海）有限公司 | Movable near-infrared imaging device |
| CN106610518A (en) * | 2015-10-23 | 2017-05-03 | 大立光电股份有限公司 | Image capture lens group, image taking device and electronic device |
| JP3208259U (en) * | 2015-10-29 | 2016-12-28 | アップル インコーポレイテッド | Near-infrared imaging lens |
| CN106483665A (en) * | 2016-12-28 | 2017-03-08 | 南开大学 | Eyepiece formula wears vein display optical system |
| CN106596497A (en) * | 2017-01-16 | 2017-04-26 | 浙江大学 | Shortwave infrared fluorescence microimaging method |
Non-Patent Citations (2)
| Title |
|---|
| CHUNYAN LI等: "In vivo real-time visualization of tissue blood flow and angiogenesis using Ag2S quantum dots in the NIR-II window", 《BIOMATERIALS》 * |
| 蔡林: "《中级摄影教程 上》", 30 April 1997, 电子科技大学出版社 * |
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Effective date of registration: 20200623 Address after: Room 1202, Yipeng science and Innovation Park, No. 888, qingliuzhong Road, Yipeng street, Qiantang New District, Hangzhou City, Zhejiang Province Applicant after: Wilmington (Hangzhou) Medical Technology Co.,Ltd. Address before: 314000 Middle Ages Apartments in Hongkong City, Nanhu District, Jiaxing City, Zhejiang Province, 2 2806 Rooms Applicant before: Wang Hu |
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Application publication date: 20181207 |